Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,476,640	A→G	I48T (ATC→ACC)	unknown ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,476,640	0	A	G	100.0%	177.8 / NA	65	I48T (ATC→ACC)	unknown	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (19/46);  total (19/46)

AAGAAGACTGATCGTCAAAAATCGTACGCGCCGACGCTTTGCCCACAATCTTCGACAACTCGTCATTGATCATATCTTTTAATAGCCGTTTTTCAAGGGCGGTAACACGTTTATCCGATTGCTTGCCCAATTCCGGTAACAAACAATATTCCAGGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCCGTGGAAATTTTGTATCTTGGATCCTGATATTTCGGTTCATGCGTGCGGCGGATGCTGAAAACGGCATGTTCTTCATCGTCATTATGCTGCACGGAAAAGGA‑CGGATAACTGGGCTCAAATTGCGGATAAGGATAATAACCCGCGCAGGCGGTTAAAATAGAAATCGGTAAAACAAGTAAGTATTTTTTTAACATGATATTCCTT‑AGGTTGAAAATAGATGAATCATTGCCTCGCTGCAGCAACCTTAAAAGTCCGAATTACGTGTAACTATATA  >  1/1476398‑1476862                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                 aagaagACTGCTCGTCAAAAATCGTGCGCGCCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGaaa                                                                                                                                                                                                                          <  1:78169/251‑1 (MQ=255)   gaagaCTGCTCGTCAAAAATCGTGCGCGCCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGaaaa                                                                                                                                                                                                                         >  1:22906/1‑250 (MQ=255)    aagaCTGCTCGTCAAAAATCGTGCGCGCCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCTTGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGaaaaag                                                                                                                                                                                                                       >  1:186546/1‑249 (MQ=255)        ctgctCGTCAAAAATCGTGCGCGCCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCAt                                                                                                                                                                                                                   >  1:219933/5‑251 (MQ=255)             cGTCAAAAATCGTGCGCGCCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCAtttt                                                                                                                                                                                                                >  1:408953/1‑246 (MQ=255)                         tgcgcgCCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTa                                                                                                                                                                                                  <  1:344240/249‑1 (MQ=255)                             cgcCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTAtgc                                                                                                                                                                                               <  1:14263/250‑1 (MQ=255)                             cgcCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTAtgc                                                                                                                                                                                               <  1:359877/250‑1 (MQ=255)                             cgcCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTAtgc                                                                                                                                                                                               <  1:841660/250‑1 (MQ=255)                             cgcCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTAtgc                                                                                                                                                                                               <  1:590613/250‑1 (MQ=255)                             cgcCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTAtgc                                                                                                                                                                                               <  1:663833/250‑1 (MQ=255)                             cgcCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTAtgc                                                                                                                                                                                               <  1:485292/250‑1 (MQ=255)                             cgcCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTAtgc                                                                                                                                                                                               <  1:311624/250‑1 (MQ=255)                               ccgatggtttGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTAtgc                                                                                                                                                                                               <  1:877428/241‑1 (MQ=255)                                   tggtttGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACgg                                                                                                                                                                                        >  1:33720/4‑251 (MQ=255)                                     gtttGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGGaa                                                                                                                                                                                      >  1:689318/2‑251 (MQ=255)                                     gtttGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGGGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGGaa                                                                                                                                                                                      >  1:99062/2‑251 (MQ=255)                                      tttGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGGaag                                                                                                                                                                                     <  1:591671/251‑2 (MQ=255)                                      tttGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGGaag                                                                                                                                                                                     <  1:585668/251‑2 (MQ=255)                                        tGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGGaaggg                                                                                                                                                                                   <  1:803649/251‑4 (MQ=255)                                           ccACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGGaagggacc                                                                                                                                                                                <  1:735009/251‑7 (MQ=255)                                                aTCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTAtgc                                                                                                                                                                                               >  1:760806/1‑231 (MQ=255)                                                   ttCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGGaagggaccggataacc                                                                                                                                                                        <  1:13011/251‑15 (MQ=255)                                                    tcgccAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGGaagggaccggataacca                                                                                                                                                                       <  1:810757/247‑16 (MQ=255)                                                          aCTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTTCTGAAAACGGCCTTTTCTTCATCATTATTATGCTGCACGGaagggggcgga                                                                                                                                                                             >  1:677864/1‑230 (MQ=255)                                                             cGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGGaagggaccggataaccaggttcaaat                                                                                                                                                              <  1:895388/251‑25 (MQ=255)                                                                    gATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGGaagggaccggataacc                                                                                                                                                                        <  1:155253/234‑15 (MQ=255)                                                                    gATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGG‑AAGGGACCGGATAACCAGGGTCAAATTGCggat                                                                                                                                                      >  1:802080/1‑251 (MQ=255)                                                                          atCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGG‑AAGGGACCGGATAACCAGGTTCAAATTGCggataaggat                                                                                                                                                >  1:474733/1‑251 (MQ=255)                                                                           tCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGGaagggaccggataacca                                                                                                                                                                       <  1:841763/228‑16 (MQ=255)                                                                             ttttAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGG‑AAGGGACCGGATAACCAGGTTCAAATTGCGGATAAGGataat                                                                                                                                             <  1:136211/251‑1 (MQ=255)                                                                              tttAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGGGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGG‑AAGGGACCGGGTAACCAGGGTCAAATTGCGGATAAGGataata                                                                                                                                            >  1:585978/1‑251 (MQ=255)                                                                               ttaataagcGTTTTTCAAGGGCAATAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGG‑AAGGGACCGGATAACCAGGTTCAAATTGCGGATAAGGAtaataa                                                                                                                                           <  1:412543/243‑1 (MQ=255)                                                                                taataagcGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGG‑AAGGGACCGGATAACCAGGTTCAAATTGCGGATAAGGAtaataa                                                                                                                                           >  1:521803/8‑250 (MQ=255)                                                                                           tttCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGG‑AAGGGACCGGATAACCAGGTTCAAATTGCGGATAAGGATAATAACCGGCACAGGCg                                                                                                                               <  1:107284/251‑1 (MQ=255)                                                                                                    cagTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGGaagggaccggataaccag                                                                                                                                                                      >  1:100859/3‑188 (MQ=255)                                                                                                    cagTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGG‑AAGGGACCGGATAACCAGGTTCAAATTGCGGATAAGGATAATAACCGGCACAGGCGGTTAAAAt                                                                                                                       <  1:421049/248‑1 (MQ=255)                                                                                                    cagTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGG‑AAGGGACCGGATAACCAGGTTCAAATTGCGGATAAGGATAATAACCGGCACAGGCGGTTAAAAt                                                                                                                       <  1:335878/248‑1 (MQ=255)                                                                                                    cagTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGG‑AAGGGACCGGATAAACAGGTTCAAATTGCGGATAAGGATAATAACCGGCACAGGCg                                                                                                                               >  1:804429/3‑242 (MQ=255)                                                                                                     aatAACACGTTCGTCCCATTTCTTTCCCAATTCCCGCAACCAACAAAATTCCAAGTTTTTTTTTGCTTAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGG‑AAGGGACCGGATAACCAGGTTCAAATTGCGGATAAGGATAATAACCGGCACAGGCGGTTAAAATAg                                                                                                                     <  1:157874/249‑1 (MQ=255)                                                                                                      gTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATc                                                                                                                                                                                                         <  1:585590/167‑1 (MQ=255)                                                                                                      gTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCAc                                                                                                                                                                                          <  1:778236/182‑1 (MQ=255)                                                                                                      gTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGG‑AAGGGACCGGATAACCAGGTTCAAATTGCGGATAAGGataata                                                                                                                                            >  1:248727/1‑227 (MQ=255)                                                                                                      gTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGG‑AAGGGACCGGATAACCAGGCTCAAATTGCGGATAAGGATAATAACCGGCACAGGCGGTTtaaaaaaa                                                                                                                    >  1:783462/1‑247 (MQ=255)                                                                                                                  tCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTAtgc                                                                                                                                                                                               <  1:417308/165‑1 (MQ=255)                                                                                                                  tCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGG‑AAGGGACCGGATAACCAGGTTCAAATTGCGGATAAGGATAATAACCGGCACAGGCGGTTAAAATAGAAATCGGTaaaat                                                                                                        <  1:126569/251‑2 (MQ=255)                                                                                                                  tCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGG‑AAGGGACCGGATAACCAGGTTCAAATTGCGGATAAGGATAATAACCGGCACAGGCGGTTAAAATAGAAATCGGTaaaat                                                                                                        <  1:267454/251‑2 (MQ=255)                                                                                                                       ttgcttgcCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGG‑AAGGGACCGGATAACCAGGTTCAAATTGCGGATAAGGATAATAACCGGCACAGGCGGTTAAAATAGAAATCGGTAAAATaagta                                                                                                   <  1:664378/251‑1 (MQ=255)                                                                                                                        tgcttgcCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGG‑AAGGGACCGGATAACCAGGTTCAAATTGCGGATAAGGATAATAACCGGCACAGGCGGTTAAAATAg                                                                                                                     <  1:162855/232‑1 (MQ=255)                                                                                                                          cttgcCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGG‑AAGGGACCGGATAACCAGGTTCAAATTGCGGATAAGGATAATAACCGGCACAGGCGGTTAAAATAGAAATCGGTAAAAtaagtaag                                                                                                 <  1:586646/250‑1 (MQ=255)                                                                                                                             gcCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTAtgc                                                                                                                                                                                               <  1:409028/154‑1 (MQ=255)                                                                                                                             gcCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGG‑AAGGGACCGGATAACCAGGTTCAAATTGCggataagga                                                                                                                                                 >  1:312469/1‑199 (MQ=255)                                                                                                                             gcCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGG‑AAGGGACCGGATAACCAGGTTCAAATTGCGGATAAGGATAATAACCGGCACAGGCGGTTAAAATAg                                                                                                                     <  1:157371/227‑1 (MQ=255)                                                                                                                                               acaaTATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGG‑AAGGGACCGGATAACCAGGTTCAAATTGCGGATAAGGATAATAACCGGCACAGGCGGTTAAAATAGAAATCGGTAAAATAAGTAAGTATTTTTTTAACATGATATTcc                                                                           <  1:417865/251‑1 (MQ=255)                                                                                                                                                caaTATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGGaagggacc                                                                                                                                                                                <  1:524781/150‑7 (MQ=255)                                                                                                                                                caaTATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGG‑AAGGGACCGGATAACCAGGTTCAAATTGCGGATAAGGATAATAACCGGCACAGGCGGTTAAAATAGAAATCGGTAAAATaag                                                                                                     <  1:318695/224‑1 (MQ=255)                                                                                                                                                  atatTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGG‑AAGGGACCGGATAACCAGGTTCAAATTGCGGATAAGGATAATAACCGGCACAGGCGGTTAAAATAg                                                                                                                     <  1:161632/206‑1 (MQ=255)                                                                                                                                                                              aaTTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGG‑AAGGGACCGGATAACCAGGTTCAAATTGCGGATAAGGATAATAACCGGCACAGGCGGTTAAAATAGAAATCGGTAAAATAAGTAAGTATTTTTTTAACATGATATTCCTTCAGGTTGAAAATAGATTAATCATTgtcc                                             <  1:23965/250‑4 (MQ=255)                                                                                                                                                                                tttttCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGG‑AAGGGACCGGATAACCAGGTTCAAATTGCGGATAAGGATAATAACCGGCACAGGCGGTTAAAATAGAAATCGGTAAAATAAGTAAGTATTTTTTTAACATGATATTCCTTCAGGTTGAAAATAGATTAATCATTgtcccgc                                          <  1:772262/251‑7 (MQ=255)                                                                                                                                                                                                             aTCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGG‑AAGGGACCGGATAACCAGGTTCAAATTGCGGATAAGGATAATAACCGGCACAGGCGGTTAAAATAGAAATCGGTAAAATAAGTAAGTATTTTTTTAACATGATATTCCTTCAGGTTGAAAATAGATTAATCATTGTCCCGCTGCAGCAACCTTAAAAGCCTGAATTACgt             <  1:215132/251‑1 (MQ=255)                                                                                                                                                                                                                   gATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGG‑AAGGGACCGGATAACCAGGTTCAAATTGCGGATAAGGATAATAACCGGCACAGGCGGTTAAAATAGAAATCGGTAAAATAAGTAAGTATTTTTTTAACATGATATTCCTTCAGGTTGAAAATAGATTAATCATTGTCCCGCTGCAGCAACCTTAAAAGCCTGAATTACGTATAAc        <  1:357354/250‑1 (MQ=255)                                                                                                                                                                                                                       cTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGG‑AAGGGACCGGATAACCAGGTTCAAATTGCGGATAAGGATAATAACCGGCACAGGCGGTTAAAATAGAAATCGGTAAAATAAGTAAGTATTTTTTTAACATGATATTCCTTCAGGTTGAAAATAGATTAATCATTGTCCCGCTGCAGCAACCTTAAAAGCCTGAATTACGTATAACcatat   <  1:231242/251‑1 (MQ=255)                                                                                                                                                                                                                       cTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGG‑AAGGGACCGGATAACCAGGTTCAAATTGCGGATAAGGATAATAACCGGCACAGGCGGTTAAAATAGAAATCGGTAAAATAAGTAAGTATTTTTTTAACATGATATTCCTTCAGGTTGAAAATAGATTAATCATTGTCCCGCTGCAGCAACCTTAAAAGCCTGAATTACGTATAACcatat   <  1:693307/251‑1 (MQ=255)                                                                                                                                                                                                                        tGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGG‑AAGGGACCGGATAACCAGGTTCAAATTGCGGATAAGGATAATAACCGGCACAGGCGGTTAAAATAGAAATCGGTAAAATAAGTAAGTATTTTTTTAACATGATATTCCTTCAGGTTGAAAATAGATTAATCATTGTCCCGCTGCAGCAACCTTAAAAGCCTGAATTACGTATAACcatata  <  1:135765/251‑1 (MQ=255)                                                                                                                                                                                                                         gATATTTCGGTTCATGCGTGCGGCGGGCGCTGAAAACGGCATTTTCTTCATCATCATTATTCTGCACGG‑AAGGGACCGGATAACCAGGTTCAAATTGCggataagga                                                                                                                                                 >  1:185424/1‑107 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                 AAGAAGACTGATCGTCAAAAATCGTACGCGCCGACGCTTTGCCCACAATCTTCGACAACTCGTCATTGATCATATCTTTTAATAGCCGTTTTTCAAGGGCGGTAACACGTTTATCCGATTGCTTGCCCAATTCCGGTAACAAACAATATTCCAGGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCCGTGGAAATTTTGTATCTTGGATCCTGATATTTCGGTTCATGCGTGCGGCGGATGCTGAAAACGGCATGTTCTTCATCGTCATTATGCTGCACGGAAAAGGA‑CGGATAACTGGGCTCAAATTGCGGATAAGGATAATAACCCGCGCAGGCGGTTAAAATAGAAATCGGTAAAACAAGTAAGTATTTTTTTAACATGATATTCCTT‑AGGTTGAAAATAGATGAATCATTGCCTCGCTGCAGCAACCTTAAAAGTCCGAATTACGTGTAACTATATA  >  1/1476398‑1476862

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑