Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,476,534	T→C	L83L (TTA→TTG)	unknown ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,476,534	0	T	C	100.0%	197.1 / NA	70	L83L (TTA→TTG)	unknown	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (26/44);  total (26/44)

TTCGTTGAGACGATCGCGAAAGTGCCGTTTCAAATCGCGGCATTCAAATTCACGAATGTTATTCACACCGTGATTGAGTTGTTGATACTTAAATTGAAAATAATCGTAAGAAGACTGATCGTCAAAAATCGTACGCGCCGACGCTTTGCCCACAATCTTCGACAACTCGTCATTGATCATATCTTTTAATAGCCGTTTTTCAAGGGCGGTAACACGTTTATCCGATTGCTTGCCCAATTCCGGTAACAAACAATATTCCAGGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCCGTGGAAATTTTGTATCTTGGATCCTGATATTTCGGTTCATGCGTGCGGCGGATGCTGAAAACGGCATGTTCTTCATCGTCATTATGCTGCACGGAAAAGGA‑CGGATAACTGGGCTCAAATTGCGGATAAGGATAATAACCCGCGCAGGCGGTTAAAATAGAAATCGGTAAAACAAGTAAG  >  1/1476291‑1476768                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                            ttcgttGAGGCGATCGCGAAAATGCCGTTTCAAATCGCGGCATTCAAATTCACGAATGTTATTCACACCGTGATTGAGTTGTTGATACTTAAATTGAAAATAATCGTAAGAAGACTGCTCGTCAAAAATCGTGCGCGCCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCaacaaac                                                                                                                                                                                                                                      <  1:347133/251‑1 (MQ=255)                 cgAAAATGCCGTTTCAAATCGCGGCATTCAAATTCACGAATGTTATTCACACCGTGATTGAGTTGTTGATACTTAAATTGAAAATAATCGTAAGAAGACTGCTCGTCAAAAATCGTGCGCGCCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTAtt                                                                                                                                                                                                                      >  1:254921/1‑251 (MQ=255)                   aaaatGCCGTTTCAAATCGCGGCATTCAAATTCACGAATGTTATTCACACCGTGATTGAGTTGTTGATACTTAAATTGAAAATAATCGTAAGAAGACTGCTCGTCAAAAATCGTGCGCGCCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTAttt                                                                                                                                                                                                                     <  1:156926/246‑1 (MQ=255)                          cGTTTCAAATCGCGGCATTCAAATTCACGAATGTTATTCACACCGTGATTGAGTTGTTGATACTTAAATTGAAAATAATCGTAAGAAGACTGCTCGTCAAAAATCGTGCGCGCCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTaa                                                                                                                                                                                                             >  1:49425/1‑251 (MQ=255)                                                         tGTTATTCACACCGTGATTGAGTTGTTGATACTTAAATTGAAAATAATCGTAAGAAGACTGCTCGTCAAAAATCGTGCGCGCCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAAtt                                                                                                                                                                              >  1:306521/1‑251 (MQ=255)                                                           ttATTCACACCGTGATTGAGTTGTTGATACTTAAATTGAAAATAATCGTAAGAAGACTGCTCGTCAAAAATCGTGCGCGCCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAAtttt                                                                                                                                                                            >  1:823312/1‑251 (MQ=255)                                                                      gTGATTGAGTTGTTGATACTTAAATTGAAAATAATCGTAAGAAGACTGCTCGTCAAAAATCGTGCGCGCCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGAt                                                                                                                                                                 >  1:676342/1‑251 (MQ=255)                                                                               ttgttgATACTTAAATTGAAAATAATCGTAAGAAGACTGCTCGTCAAAAATCGTGCGCGCCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGatat                                                                                                                                                         <  1:610534/250‑1 (MQ=255)                                                                                           aaaTTGAAAATAATCGTAAGAAGACTGCTCGTCAAAAATCGTGCGCGCCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCAtgcg                                                                                                                                            <  1:869396/251‑1 (MQ=255)                                                                                           aaaTTGAAAATAATCGTAAGAAGACTGCTCGTCAAAAATCGTGCGCGCCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCAtgcg                                                                                                                                            <  1:195905/251‑1 (MQ=255)                                                                                                         cGTAAGAAGACTGCTCGTCAAAAATCGTGCGCGCCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTcc                                                                                                                                                                                                                              >  1:597034/1‑155 (MQ=255)                                                                                                           tAAGAAGACTGCTCGTCAAAAATCGTGCGCGCCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAAtt                                                                                                                                                                              >  1:623237/1‑201 (MQ=255)                                                                                                           tAAGAAGACTGCTCGTCAAAAATCGTGCGCGCCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCAtgcg                                                                                                                                            <  1:536522/235‑1 (MQ=255)                                                                                                           tAAGAAGACTGCTCGTCAAAAATCGTGCGCGCCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGcggcgggtg                                                                                                                                 <  1:816875/246‑4 (MQ=255)                                                                                                            aagaagACTGCTCGTCAAAAATCGTGCGCGCCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGaaa                                                                                                                           <  1:78169/251‑1 (MQ=255)                                                                                                              gaagaCTGCTCGTCAAAAATCGTGCGCGCCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGaaaa                                                                                                                          >  1:22906/1‑250 (MQ=255)                                                                                                               aagaCTGCTCGTCAAAAATCGTGCGCGCCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCTTGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGaaaaag                                                                                                                        >  1:186546/1‑249 (MQ=255)                                                                                                                   ctgctCGTCAAAAATCGTGCGCGCCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCAt                                                                                                                    >  1:219933/5‑251 (MQ=255)                                                                                                                        cGTCAAAAATCGTGCGCGCCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCAtttt                                                                                                                 >  1:408953/1‑246 (MQ=255)                                                                                                                                    tgcgcgCCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTa                                                                                                   <  1:344240/249‑1 (MQ=255)                                                                                                                                        cgcCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTAtgc                                                                                                <  1:841660/250‑1 (MQ=255)                                                                                                                                        cgcCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTAtgc                                                                                                <  1:311624/250‑1 (MQ=255)                                                                                                                                        cgcCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTAtgc                                                                                                <  1:590613/250‑1 (MQ=255)                                                                                                                                        cgcCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTAtgc                                                                                                <  1:14263/250‑1 (MQ=255)                                                                                                                                        cgcCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTAtgc                                                                                                <  1:663833/250‑1 (MQ=255)                                                                                                                                        cgcCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTAtgc                                                                                                <  1:359877/250‑1 (MQ=255)                                                                                                                                        cgcCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTAtgc                                                                                                <  1:485292/250‑1 (MQ=255)                                                                                                                                          ccgatggtttGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTAtgc                                                                                                <  1:877428/241‑1 (MQ=255)                                                                                                                                              tggtttGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACgg                                                                                         >  1:33720/4‑251 (MQ=255)                                                                                                                                                gtttGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGGaa                                                                                       >  1:689318/2‑251 (MQ=255)                                                                                                                                                gtttGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGGGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGGaa                                                                                       >  1:99062/2‑251 (MQ=255)                                                                                                                                                 tttGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGGaag                                                                                      <  1:585668/251‑2 (MQ=255)                                                                                                                                                 tttGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGGaag                                                                                      <  1:591671/251‑2 (MQ=255)                                                                                                                                                   tGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGGaaggg                                                                                    <  1:803649/251‑4 (MQ=255)                                                                                                                                                      ccACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGGaagggacc                                                                                 <  1:735009/251‑7 (MQ=255)                                                                                                                                                           aTCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTAtgc                                                                                                >  1:760806/1‑231 (MQ=255)                                                                                                                                                              ttCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGGaagggaccggataacc                                                                         <  1:13011/251‑15 (MQ=255)                                                                                                                                                               tcgccAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGGaagggaccggataacca                                                                        <  1:810757/247‑16 (MQ=255)                                                                                                                                                                 gccAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTcc                                                                                                                                                                                                                              <  1:816754/97‑1 (MQ=255)                                                                                                                                                                     aCTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTTCTGAAAACGGCCTTTTCTTCATCATTATTATGCTGCACGGaagggggcgga                                                                              >  1:677864/1‑230 (MQ=255)                                                                                                                                                                        cGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGGaagggaccggataaccaggttcaaat                                                               <  1:895388/251‑25 (MQ=255)                                                                                                                                                                               gATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGat                                                                                                                                                           <  1:694235/152‑1 (MQ=255)                                                                                                                                                                               gATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGGaagggaccggataacc                                                                         <  1:155253/234‑15 (MQ=255)                                                                                                                                                                               gATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGG‑AAGGGACCGGATAACCAGGGTCAAATTGCggat                                                       >  1:802080/1‑251 (MQ=255)                                                                                                                                                                                     atCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGG‑AAGGGACCGGATAACCAGGTTCAAATTGCggataaggat                                                 >  1:474733/1‑251 (MQ=255)                                                                                                                                                                                      tCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGGaagggaccggataacca                                                                        <  1:841763/228‑16 (MQ=255)                                                                                                                                                                                        ttttAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGG‑AAGGGACCGGATAACCAGGTTCAAATTGCGGATAAGGataat                                              <  1:136211/251‑1 (MQ=255)                                                                                                                                                                                         tttAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGGGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGG‑AAGGGACCGGGTAACCAGGGTCAAATTGCGGATAAGGataata                                             >  1:585978/1‑251 (MQ=255)                                                                                                                                                                                          ttaataagcGTTTTTCAAGGGCAATAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGG‑AAGGGACCGGATAACCAGGTTCAAATTGCGGATAAGGAtaataa                                            <  1:412543/243‑1 (MQ=255)                                                                                                                                                                                           taataagcGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGG‑AAGGGACCGGATAACCAGGTTCAAATTGCGGATAAGGAtaataa                                            >  1:521803/8‑250 (MQ=255)                                                                                                                                                                                                      tttCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTGTCTCGGATCCTGatat                                                                                                                                                         >  1:302209/1‑131 (MQ=255)                                                                                                                                                                                                      tttCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGG‑AAGGGACCGGATAACCAGGTTCAAATTGCGGATAAGGATAATAACCGGCACAGGCg                                <  1:107284/251‑1 (MQ=255)                                                                                                                                                                                                               cagTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGGaagggaccggataaccag                                                                       >  1:100859/3‑188 (MQ=255)                                                                                                                                                                                                               cagTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGG‑AAGGGACCGGATAACCAGGTTCAAATTGCGGATAAGGATAATAACCGGCACAGGCGGTTAAAAt                        <  1:335878/248‑1 (MQ=255)                                                                                                                                                                                                               cagTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGG‑AAGGGACCGGATAACCAGGTTCAAATTGCGGATAAGGATAATAACCGGCACAGGCGGTTAAAAt                        <  1:421049/248‑1 (MQ=255)                                                                                                                                                                                                               cagTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGG‑AAGGGACCGGATAAACAGGTTCAAATTGCGGATAAGGATAATAACCGGCACAGGCg                                >  1:804429/3‑242 (MQ=255)                                                                                                                                                                                                                aatAACACGTTCGTCCCATTTCTTTCCCAATTCCCGCAACCAACAAAATTCCAAGTTTTTTTTTGCTTAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGG‑AAGGGACCGGATAACCAGGTTCAAATTGCGGATAAGGATAATAACCGGCACAGGCGGTTAAAATAg                      <  1:157874/249‑1 (MQ=255)                                                                                                                                                                                                                 gTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATc                                                                                                          <  1:585590/167‑1 (MQ=255)                                                                                                                                                                                                                 gTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCAc                                                                                           <  1:778236/182‑1 (MQ=255)                                                                                                                                                                                                                 gTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGG‑AAGGGACCGGATAACCAGGTTCAAATTGCGGATAAGGataata                                             >  1:248727/1‑227 (MQ=255)                                                                                                                                                                                                                 gTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGG‑AAGGGACCGGATAACCAGGCTCAAATTGCGGATAAGGATAATAACCGGCACAGGCGGTTtaaaaaaa                     >  1:783462/1‑247 (MQ=255)                                                                                                                                                                                                                             tCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTAtgc                                                                                                <  1:417308/165‑1 (MQ=255)                                                                                                                                                                                                                             tCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGG‑AAGGGACCGGATAACCAGGTTCAAATTGCGGATAAGGATAATAACCGGCACAGGCGGTTAAAATAGAAATCGGTaaaat         <  1:267454/251‑2 (MQ=255)                                                                                                                                                                                                                             tCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGG‑AAGGGACCGGATAACCAGGTTCAAATTGCGGATAAGGATAATAACCGGCACAGGCGGTTAAAATAGAAATCGGTaaaat         <  1:126569/251‑2 (MQ=255)                                                                                                                                                                                                                                  ttgcttgcCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGG‑AAGGGACCGGATAACCAGGTTCAAATTGCGGATAAGGATAATAACCGGCACAGGCGGTTAAAATAGAAATCGGTAAAATaagta    <  1:664378/251‑1 (MQ=255)                                                                                                                                                                                                                                   tgcttgcCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGG‑AAGGGACCGGATAACCAGGTTCAAATTGCGGATAAGGATAATAACCGGCACAGGCGGTTAAAATAg                      <  1:162855/232‑1 (MQ=255)                                                                                                                                                                                                                                     cttgcCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGG‑AAGGGACCGGATAACCAGGTTCAAATTGCGGATAAGGATAATAACCGGCACAGGCGGTTAAAATAGAAATCGGTAAAAtaagtaag  <  1:586646/250‑1 (MQ=255)                                                                                                                                                                                                                                        gcCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTAtgc                                                                                                <  1:409028/154‑1 (MQ=255)                                                                                                                                                                                                                                        gcCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGG‑AAGGGACCGGATAACCAGGTTCAAATTGCggataagga                                                  >  1:312469/1‑199 (MQ=255)                                                                                                                                                                                                                                        gcCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGG‑AAGGGACCGGATAACCAGGTTCAAATTGCGGATAAGGATAATAACCGGCACAGGCGGTTAAAATAg                      <  1:157371/227‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                            TTCGTTGAGACGATCGCGAAAGTGCCGTTTCAAATCGCGGCATTCAAATTCACGAATGTTATTCACACCGTGATTGAGTTGTTGATACTTAAATTGAAAATAATCGTAAGAAGACTGATCGTCAAAAATCGTACGCGCCGACGCTTTGCCCACAATCTTCGACAACTCGTCATTGATCATATCTTTTAATAGCCGTTTTTCAAGGGCGGTAACACGTTTATCCGATTGCTTGCCCAATTCCGGTAACAAACAATATTCCAGGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCCGTGGAAATTTTGTATCTTGGATCCTGATATTTCGGTTCATGCGTGCGGCGGATGCTGAAAACGGCATGTTCTTCATCGTCATTATGCTGCACGGAAAAGGA‑CGGATAACTGGGCTCAAATTGCGGATAAGGATAATAACCCGCGCAGGCGGTTAAAATAGAAATCGGTAAAACAAGTAAG  >  1/1476291‑1476768

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑