Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,476,452	A→C	S111A (TCG→GCG)	unknown ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,476,452	0	A	C	100.0%	185.8 / NA	66	S111A (TCG→GCG)	unknown	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (30/36);  total (30/36)

ACGCGTCGTCTATCGTTTGTCGTTGCTTTTGCACTTGGTTTTCAACAGAACGACTGTCGCCCGCCAAGCCTTGCCGGCGTTTGCGTTCGTTGAGACGATCGCGAAAGTGCCGTTTCAAATCGCGGCATTCAAATTCACGAATGTTATTCACACCGTGATTGAGTTGTTGATACTTAAATTGAAAATAATCGTAAGAAGACTGATCGTCAAAAATCGTACGCGCCGACGCTTTGCCCACAATCTTCGACAACTCGTCATTGATCATATCTTTTAATAGCCGTTTTTCAAGGGCGGTAACACGTTTATCCGATTGCTTGCCCAATTCCGGTAACAAACAATATTCCAGGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCCGTGGAAATTTTGTATCTTGGATCCTGATATTTCGGTTCATGCGTGCGGCGGATGCTGAAAACGGCATGTTCTTCATCGTCATTATGCTGCACGGAAAAGGACGGATAACT  >  1/1476206‑1476698                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                       aCGCATCGTCTATCGTTTGTCGTTGCTTCTGCATTTGGTTTTCAACGGAACGACTGTCACCCGCCAAGCCTTGACGGCGTTTACGTTCGTTGAGGCGATCGCGAAAATGCCGTTTCAAATCGCGGCATTCAAATTCACGAATGTTATTCACACCGTGATTGAGTTGTTGATACTTAAATTGAAAATAATCGTAAGAAGACTGCTCGTCAAAAATCGTGCGCGCCGATGGTTTGCCCACAATCTTCGCCaa                                                                                                                                                                                                                                                     >  1:379285/1‑250 (MQ=255)       cgtcTATCGTTTGTCGTTGCTTCTGCATTTGGTTTTCAACGGAACGACTGTCACCCGCCAAGCCTTGACGGCGTTTACGTTCGTTGAGGCGATCGCGAAAATGCCGTTTCAAATCGCGGCATTCAAATTCACGAATGTTATTCACACCGTGATTGAGTTGTTGATACTTAAATTGAAAATAATCGTAAGAAGACTGCTCGTCAAAAATCGTGCGCGCCGATGGTTTGCCCACAATCTTCGCCAACTCGTCa                                                                                                                                                                                                                                              >  1:696428/1‑251 (MQ=255)        gtcTATCGTTTGTCGTTGCTTCTGCATTTGGTTTTCAACGGAACGACTGTCACCCGCCAAGCCTTGACGGCGTTTACGTTCGTTGAGGCGATCGCGAAAATGCCGTTTCAAATCGCGGCATTCAAATTCACGAATGTTATTCACACCGTGATTGAGTTGTTGATACTTAAATTGAAAATAATCGTAAGAAGACTGCTCGTCAAAAATCGTGCGCGCCGATGGTTTGCCCACAATCTTCGCCAACTCGTCAt                                                                                                                                                                                                                                             >  1:839783/1‑251 (MQ=255)        gtcTATCGTTTGTCGTTGCTTCTGCATTTGGTTTTCAACGGAACGACTGTCACCCGCCAAGCCTTGACGGCGTTTACGTTCGTTGAGGCGATCGCGAAAATGCCGTTTCAAATCGCGGCATTCAAATTCACGAATGTTATTCACACCGTGATTGAGTTGTTGATACTTAAATTGAAAATAATCGTAAGAAGACTGCTCGTCAAAAATCGTGCGCGCCGATGGTTTGCCCACAATCTTCGCCAACTCGTCAt                                                                                                                                                                                                                                             >  1:561636/1‑251 (MQ=255)           tATCGTTTGTCGTTGCTTCTGCATTTGGTTTTCAACGGAACGACTGTCACCCGCCAAGCCTTGACGGCGTTTACGTTCGTTGAGGCGATCGCGAAAATGCCGTTTCAAATCGCGGCATTCAAATTCACGAATGTTATTCACACCGTGATTGAGTTGTTGATACTTAAATTGAAAATAATCGTAAGAAGACTGCTCGTCAAAAATCGTGCGCGCCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGa                                                                                                                                                                                                                                          <  1:652731/251‑1 (MQ=255)            aTCGTTTGTCGTTGCTTCTGCATTTGGTTTTCAACGGAACGACTGTCACCCGCCAAGCCTTGACGGCGTTTACGTTCGTTGAGGCGATCGCGAAAATGCCGTTTCAAATCGCGGCATTCAAATTCACGAATGTTATTCACACCGTGATTGAGTTGTTGATACTTAAATTGAAAATAATCGTAAGAAGACTGCTCGTCAAAAATCGTGCGCGCCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGAt                                                                                                                                                                                                                                         <  1:796636/251‑1 (MQ=255)             tCGTTTGTCGTTGCTTCTGCATTTGGTTTTCAACGGAACGACTGTCACCCGCCAAGCCTTGACGGCGTTTACGTTCGTTGAGGCGATCGCGAAAATGCCGTTTCAAATCGCGGCATTCAAATTCACGAATGTTATTCACACCGTGATTGAGTTGTTGATACTTAAATTGAAAATAATCGTAAGAAGACTGCTCGTCAAAAATCGTGCGCGCCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATc                                                                                                                                                                                                                                        <  1:839317/251‑1 (MQ=255)             tCGTTTGTCGTTGCTTCTGCATTTGGTTTTCAACGGAACGACTGTCACCCGCCAAGCCTTGACGGCGTTTACGTTCGTTGAGGCGATCGCGAAAATGCCGTTTCAAATCGCGGCATTCAAATTCACGAATGTTATTCACACCGTGATTGAGTTGTTGATACTTAAATTGAAAATAATCGTAAGAAGACTGCTCGTCAAAAATCGTGCGCGCCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATc                                                                                                                                                                                                                                        <  1:797376/251‑1 (MQ=255)                 ttGTCGTTGCTTCTGCATTTGGTTTTCAACGGAACGACTGTCACCCGCCAAGCCTTGACGGCGTTTACGTTCGTTGAGGCGATCGCGAAAATGCCGTTTCAAATCGCGGCATTCAAATTCACGAATGTTATTCACACCGTGATTGAGTTGTTGATACTTAAATTGAAAATAATCGTAAGAAGACTGCTCGTCAAAAATCGTGCGCGCCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCatat                                                                                                                                                                                                                                    >  1:634074/1‑251 (MQ=255)                  tGTCGTTGCTTCTGCATTTGGTTTTCAACGGAACGACTGTCACCCGCCAAGCCTTGACGGCGTTTACGTTCGTTGAGGCGATCGCGAAAATGCCGTTTCAAATCGCGGCATTCAAATTCACGAATGTTATTCACACCGTGATTGAGTTGTTGATACTTAAATTGAAAATAATCGTAAGAAGACTGCTCGTCAAAAATCGTGCGCGCCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATc                                                                                                                                                                                                                                   <  1:14135/251‑1 (MQ=255)                                    tGGTTTTCAACGGAACGACTGTCACCCGCCAAGCCTTGACGGCGTTTACGTTCGTTGAGGCGATCGCGAAAATGCCGTTTCAAATCGCGGCATTCAAATTCACGAATGTTATTCACACCGTGATTGAGTTGTTGATACTTAAATTGAAAATAATCGTAAGAAGACTGCTCGTCAAAAATCGTGCGCGCCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCatat                                                                                                                                                                                                                                    >  1:90385/1‑232 (MQ=255)                                      gTTTTCAACGGAACGACTGTCACCCGCCAAGCCTTGACGGCGTTTACGTTCGTTGAGGCGATCGCGAAAATGCCGTTTCAAATCGCGGCATTCAAATTCACGAATGTTATTCACACCGTGATTGAGTTGTTGATACTTAAATTGAAAATAATCGTAAGAAGACTGCTCGTCAAAAATCGTGCGCGCCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCaa                                                                                                                                                                                                               >  1:826907/1‑251 (MQ=255)                                          tCAACGGAACGACTGTCACCCGCCAAGCCTTGACGGCGTTTACGTTCGTTGAGGCGATCGCGAAAATGCCGTTTCAAATCGCGGCATTCAAATTCACGAATGTTATTCACACCGTGATTGAGTTGTTGATACTTAAATTGAAAATAATCGTAAGAAGACTGCTCGTCAAAAATCGTGCGCGCCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGc                                                                                                                                                                                                           <  1:350079/251‑1 (MQ=255)                                                  aCGACTGTCACCCGCCAAGCCTTGACGGCGTTTACGTTCGTTGAGGCGATCGCGAAAATGCCGTTTCAAATCGCGGCATTCAAATTCACGAATGTTATTCACACCGTGATTGAGTTGTTGATACTTAAATTGAAAATAATCGTAAGAAGACTGCTCGTCAAAAATCGTGCGCGCCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAacac                                                                                                                                                                                                   <  1:562384/251‑1 (MQ=255)                                                     aCTGTCCCCCGCCAAGCCTTGACGGCGTTTACGTTCGTTGAGGCGATCGCGAAAATGCCGTTTCAAATCGCGGCATTCAAATTCACGAATGTTATTCACACCGTGATTGAGTTGTTGATACTTAAATTGAAAATAATCGTAAGAAGACTGCTCGTCAAAAATCGTGCGCGCCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGt                                                                                                                                                                                                 <  1:558556/250‑1 (MQ=255)                                                     aCTGTCACCCGCCAAGCCTTGACGGCGTTTACGTTCGTTGAGGCGATCGCGAAAATGCCGTTTCAAATCGCGGCATTCAAATTCACGAATGTTATTCACACCGTGATTGAGTTGTTGATACTTAAATTGAAAATAATCGTAAGAAGACTGCTCGTCAAAAATCGTGCGCGCCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGtt                                                                                                                                                                                                <  1:624029/251‑1 (MQ=255)                                                     aCTGTCACCCGCCAAGCCTTGACGGCGTTTACGTTCGTTGAGGCGATCGCGAAAATGCCGTTTCAAATCGCGGCATTCAAATTCACGAATGTTATTCACACCGTGATTGAGTTGTTGATACTTAAATTGAAAATAATCGTAAGAAGACTGCTCGTCAAAAATCGTGCGCGCCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGt                                                                                                                                                                                                 <  1:226126/250‑1 (MQ=255)                                                                             gCGTTTGCGTTCGTTGAGGCGATCGCGAAAATGCCGTTTCAAATCGCGGCATTCAAATTCACGAATGTTATTCATGCCGTGATTGAGTTGTTGATTCTTAAATTGAAAATGATCGTAAGAAGTCTGCACGTCAAAAATCATGCGCGCCGAAGTTTTGCCCACAATCTTCGCAAACTCGTCATTGATCATATCTTTTAATa                                                                                                                                                                                                                           <  1:27893/200‑1 (MQ=255)                                                                                      ttcgttGAGGCGATCGCGAAAATGCCGTTTCAAATCGCGGCATTCAAATTCACGAATGTTATTCACACCGTGATTGAGTTGTTGATACTTAAATTGAAAATAATCGTAAGAAGACTGCTCGTCAAAAATCGTGCGCGCCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCaacaaac                                                                                                                                                               <  1:347133/251‑1 (MQ=255)                                                                                            gaggcGATCGCGAAAATGCCGTTTCAAATCGCGGCATTCAAATTCACGAATGTTATTCACACCGTGATTGAGTTGTTGATACTTAAATTGAAAATAATCGTAAGAAGACTGCTCGTCAAAAATCGTGCGCGCCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGt                                                                                                                                                                                                 >  1:707499/5‑211 (MQ=255)                                                                                              ggcGATCGCGAAAATGCCGTTTCAAATCGCGGCATTCAAATTCACGAATGTTATTCACACCGTGATTGAGTTGTTGATACTTAAATTGAAAATAATCGTAAGAAGACTGCTCGTCAAAAATCGTGCGCGCCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTc                                                                                                                                                                          >  1:827179/3‑232 (MQ=255)                                                                                                  aTCGCGAAAATGCCGTTTCAAATCGCGGCATTCAAATTCACGAATGTTATTCACACCGTGATTGAGTTGTTGATACTTAAATTGAAAATAATCGTAAGAAGACTGCTCGTCAAAAATCGTGCGCGCCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGtt                                                                                                                                                                                                >  1:119193/1‑206 (MQ=255)                                                                                                      cgAAAATGCCGTTTCAAATCGCGGCATTCAAATTCACGAATGTTATTCACACCGTGATTGAGTTGTTGATACTTAAATTGAAAATAATCGTAAGAAGACTGCTCGTCAAAAATCGTGCGCGCCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTAtt                                                                                                                                               >  1:254921/1‑251 (MQ=255)                                                                                                        aaaatGCCGTTTCAAATCGCGGCATTCAAATTCACGAATGTTATTCACACCGTGATTGAGTTGTTGATACTTAAATTGAAAATAATCGTAAGAAGACTGCTCGTCAAAAATCGTGCGCGCCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTAttt                                                                                                                                              <  1:156926/246‑1 (MQ=255)                                                                                                               cGTTTCAAATCGCGGCATTCAAATTCACGAATGTTATTCACACCGTGATTGAGTTGTTGATACTTAAATTGAAAATAATCGTAAGAAGACTGCTCGTCAAAAATCGTGCGCGCCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTaa                                                                                                                                      >  1:49425/1‑251 (MQ=255)                                                                                                                             gCATTCAAATTCACGAATGTTATTCACACCGTGATTGAGTTGTTGATACTTAAATTGAAAATAATCGTAAGAAGACTGCTCGTCAAAAATCGTGCGCGCCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATa                                                                                                                                                                                                                           >  1:427334/1‑152 (MQ=255)                                                                                                                             gCATTCAAATTCACGAATGTTATTCACACCGTGATTGAGTTGTTGATACTTAAATTGAAAATAATCGTAAGAAGACTGCTCGTCAAAAATCGTGCGCGCCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGttcgtc                                                                                                                                                                                            <  1:239162/183‑5 (MQ=255)                                                                                                                             gCATTCAAATTCACGAATGTTATTCACACCGTGATTGAGTTGTTGATACTTAAATTGAAAATAATCGTAAGAAGACTGCTCGTCAAAAATCGTGCGCGCCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGt                                                                                                                                                                                                 >  1:129612/1‑178 (MQ=255)                                                                                                                               aTTCAAATTCACGAATGTTATTCACACCGTGATTGAGTTGTTGATACTTAAATTGAAAATAATCGTAAGAAGACTGCTCGTCAAAAATCGTGCGCGCCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGt                                                                                                                                                                                                 >  1:421098/1‑176 (MQ=255)                                                                                                                                             aTGTTATTCACACCGTGATTGAGTTGTTGATACTTAAATTGAAAATAATCGTAAGAAGACTGCTCGTCAAAAATCGTGCGCGCCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCat                                                                                                                                                                                                                                      <  1:465626/125‑1 (MQ=255)                                                                                                                                              tGTTATTCACACCGTGATTGAGTTGTTGATACTTAAATTGAAAATAATCGTAAGAAGACTGCTCGTCAAAAATCGTGCGCGCCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAAtt                                                                                                       >  1:306521/1‑251 (MQ=255)                                                                                                                                               gTTATTCACACCGTGATTGAGTTGTTGATACTTAAATTGAAAATAATCGTAAGAAGACTGCTCGTCAAAAATCGTGCGCGCCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAggg                                                                                                                                                                                                            >  1:63035/1‑149 (MQ=255)                                                                                                                                                ttATTCACACCGTGATTGAGTTGTTGATACTTAAATTGAAAATAATCGTAAGAAGACTGCTCGTCAAAAATCGTGCGCGCCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAAtttt                                                                                                     >  1:823312/1‑251 (MQ=255)                                                                                                                                                           gTGATTGAGTTGTTGATACTTAAATTGAAAATAATCGTAAGAAGACTGCTCGTCAAAAATCGTGCGCGCCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGa                                                                                                                                                                                                                                          >  1:422230/1‑107 (MQ=255)                                                                                                                                                           gTGATTGAGTTGTTGATACTTAAATTGAAAATAATCGTAAGAAGACTGCTCGTCAAAAATCGTGCGCGCCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGAt                                                                                          >  1:676342/1‑251 (MQ=255)                                                                                                                                                                    ttgttgATACTTAAATTGAAAATAATCGTAAGAAGACTGCTCGTCAAAAATCGTGCGCGCCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGatat                                                                                  <  1:610534/250‑1 (MQ=255)                                                                                                                                                                                aaaTTGAAAATAATCGTAAGAAGACTGCTCGTCAAAAATCGTGCGCGCCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCAtgcg                                                                     <  1:869396/251‑1 (MQ=255)                                                                                                                                                                                aaaTTGAAAATAATCGTAAGAAGACTGCTCGTCAAAAATCGTGCGCGCCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCAtgcg                                                                     <  1:195905/251‑1 (MQ=255)                                                                                                                                                                                              cGTAAGAAGACTGCTCGTCAAAAATCGTGCGCGCCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTcc                                                                                                                                                       >  1:597034/1‑155 (MQ=255)                                                                                                                                                                                                tAAGAAGACTGCTCGTCAAAAATCGTGCGCGCCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAAtt                                                                                                       >  1:623237/1‑201 (MQ=255)                                                                                                                                                                                                tAAGAAGACTGCTCGTCAAAAATCGTGCGCGCCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCAtgcg                                                                     <  1:536522/235‑1 (MQ=255)                                                                                                                                                                                                tAAGAAGACTGCTCGTCAAAAATCGTGCGCGCCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGcggcgggtg                                                          <  1:816875/246‑4 (MQ=255)                                                                                                                                                                                                 aagaagACTGCTCGTCAAAAATCGTGCGCGCCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGaaa                                                    <  1:78169/251‑1 (MQ=255)                                                                                                                                                                                                   gaagaCTGCTCGTCAAAAATCGTGCGCGCCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGaaaa                                                   >  1:22906/1‑250 (MQ=255)                                                                                                                                                                                                    aagaCTGCTCGTCAAAAATCGTGCGCGCCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCTTGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGaaaaag                                                 >  1:186546/1‑249 (MQ=255)                                                                                                                                                                                                        ctgctCGTCAAAAATCGTGCGCGCCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCAt                                             >  1:219933/5‑251 (MQ=255)                                                                                                                                                                                                             cGTCAAAAATCGTGCGCGCCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCAtttt                                          >  1:408953/1‑246 (MQ=255)                                                                                                                                                                                                                  aaaaTCGTGCGCGCCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCggca                                                                                                                                                                     <  1:520153/121‑3 (MQ=255)                                                                                                                                                                                                                         tgcgcgCCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTa                            <  1:344240/249‑1 (MQ=255)                                                                                                                                                                                                                             cgcCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTAtgc                         <  1:311624/250‑1 (MQ=255)                                                                                                                                                                                                                             cgcCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTAtgc                         <  1:14263/250‑1 (MQ=255)                                                                                                                                                                                                                             cgcCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTAtgc                         <  1:485292/250‑1 (MQ=255)                                                                                                                                                                                                                             cgcCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTAtgc                         <  1:841660/250‑1 (MQ=255)                                                                                                                                                                                                                             cgcCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTAtgc                         <  1:663833/250‑1 (MQ=255)                                                                                                                                                                                                                             cgcCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTAtgc                         <  1:359877/250‑1 (MQ=255)                                                                                                                                                                                                                             cgcCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTAtgc                         <  1:590613/250‑1 (MQ=255)                                                                                                                                                                                                                               ccgatggtttGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTAtgc                         <  1:877428/241‑1 (MQ=255)                                                                                                                                                                                                                                   tggtttGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACgg                  >  1:33720/4‑251 (MQ=255)                                                                                                                                                                                                                                     gtttGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGGaa                >  1:689318/2‑251 (MQ=255)                                                                                                                                                                                                                                     gtttGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGGGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGGaa                >  1:99062/2‑251 (MQ=255)                                                                                                                                                                                                                                      tttGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGGaag               <  1:591671/251‑2 (MQ=255)                                                                                                                                                                                                                                      tttGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGGaag               <  1:585668/251‑2 (MQ=255)                                                                                                                                                                                                                                        tGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGGaaggg             <  1:803649/251‑4 (MQ=255)                                                                                                                                                                                                                                           ccACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGGaagggacc          <  1:735009/251‑7 (MQ=255)                                                                                                                                                                                                                                                aTCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTAtgc                         >  1:760806/1‑231 (MQ=255)                                                                                                                                                                                                                                                   ttCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCATTTTCTTCATCATCATTATGCTGCACGGaagggaccggataacc  <  1:13011/251‑15 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                       ACGCGTCGTCTATCGTTTGTCGTTGCTTTTGCACTTGGTTTTCAACAGAACGACTGTCGCCCGCCAAGCCTTGCCGGCGTTTGCGTTCGTTGAGACGATCGCGAAAGTGCCGTTTCAAATCGCGGCATTCAAATTCACGAATGTTATTCACACCGTGATTGAGTTGTTGATACTTAAATTGAAAATAATCGTAAGAAGACTGATCGTCAAAAATCGTACGCGCCGACGCTTTGCCCACAATCTTCGACAACTCGTCATTGATCATATCTTTTAATAGCCGTTTTTCAAGGGCGGTAACACGTTTATCCGATTGCTTGCCCAATTCCGGTAACAAACAATATTCCAGGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCCGTGGAAATTTTGTATCTTGGATCCTGATATTTCGGTTCATGCGTGCGGCGGATGCTGAAAACGGCATGTTCTTCATCGTCATTATGCTGCACGGAAAAGGACGGATAACT  >  1/1476206‑1476698

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑