Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,476,423	A→G	R120R (CGT→CGC)	unknown ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,476,423	0	A	G	100.0%	147.5 / NA	54	R120R (CGT→CGC)	unknown	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (29/25);  total (29/25)

TTCTCGTTGCGTGTGAAAACGGCGAAACGCGTCGTCTATCGTTTGTCGTTGCTTTTGCACTTGGTTTTCAACAGAACGACTGTCGCCCGCCAAGCCTTGCCGGCGTTTGCGTTCGTTGAGACGATCGCGAAAGTGCCGTTTCAAATCGCGGCATTCAAATTCACGAATGTTATTCACACCGTGATTGAGTTGTTGATACTTAAATTGAAAATAATCGTAAGAAGACTGATCGTCAAAAATCGTACGCGCCGACGCTTTGCCCACAATCTTCGACAACTCGTCATTGATCATATCTTTTAATAGCCGTTTTTCAAGGGCGGTAACACGTTTATCCGATTGCTTGCCCAATTCCGGTAACAAACAATATTCCAGGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCCGTGGAAATTTTGTATCTTGGATCCTGATATTTCGGTTCATGCGTGCGGCGGATGCTGAAAACGGCATGTT  >  1/1476180‑1476658                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                            ttCTCGTTGCGTGTGAAAACGGCGAAACGCATCGTCTATCGTTTGTCGTTGCTTCTGCATTTGGTTTTCAACGGAACGACTGTCACCCGCCAAGCCTTGACGGCGTTTACGTTCGTTGAGGCGATCGCGAAAATGCCGTTTCAAATCGCGGCATTCAAATTCACGAATGTTATTCACACCGTGATTGAGTTGTTGATACTTAAATTGAAAATAATCGTAAGAAGACTGCTCGTCAAAAATCGTGCGCGCCg                                                                                                                                                                                                                                      >  1:93155/1‑251 (MQ=255)   ctcGTTGCGTGTGAAAACGGCGAAACGCATCGTCTATCGTTTGTCGTTGCTTCTGCATTTGGTTTTCAACGGAACGACTGTCACCCGCCAAGCCTTGACGGCGTTTACGTTCGTTGAGGCGATCGCGAAAATGCCGTTTCAAATCGCGGCATTCAAATTCACGAATGTTATTCACACCGTGATTGAGTTGTTGATACTTAAATTGAAAATAATCGTAAGAAGACTGCTCGTCAAAAATCGTGCGCGCCGat                                                                                                                                                                                                                                    >  1:169170/1‑250 (MQ=255)                  aaCGGCGAAACGCATCGTCTATCGTTTGTCGTTGCTTCTGCATTTGGTTTTCAACGGAACGACTGTCACCCGCCAAGCCTTGACGGCGTTTACGTTCGTTGAGGCGATCGCGAAAATGCCGTTTCAAATCGCGGCATTCAAATTCACGAATGTTATTCACACCGTGATTGAGTTGTTGATACTTAAATTGAAAATAATCGTAAGAAGACTGCTCGTCAAAAATCGTGCGCGCCGATGGTTTGCCCACAATc                                                                                                                                                                                                                     <  1:691709/251‑1 (MQ=255)                           aCGCATCGTCTATCGTTTGTCGTTGCTTCTGCATTTGGTTTTCAACGGAACGACTGTCACCCGCCAAGCCTTGACGGCGTTTACGTTCGTTGAGGCGATCGCGAAAATGCCGTTTCAAATCGCGGCATTCAAATTCACGAATGTTATTCACACCGTGATTGAGTTGTTGATACTTAAATTGAAAATAATCGTAAGAAGACTGCTCGTCAAAAATCGTGCGCGCCGATGGTTTGCCCACAATCTTCGCCaa                                                                                                                                                                                                             >  1:379285/1‑250 (MQ=255)                                 cgtcTATCGTTTGTCGTTGCTTCTGCATTTGGTTTTCAACGGAACGACTGTCACCCGCCAAGCCTTGACGGCGTTTACGTTCGTTGAGGCGATCGCGAAAATGCCGTTTCAAATCGCGGCATTCAAATTCACGAATGTTATTCACACCGTGATTGAGTTGTTGATACTTAAATTGAAAATAATCGTAAGAAGACTGCTCGTCAAAAATCGTGCGCGCCGATGGTTTGCCCACAATCTTCGCCAACTCGTCa                                                                                                                                                                                                      >  1:696428/1‑251 (MQ=255)                                  gtcTATCGTTTGTCGTTGCTTCTGCATTTGGTTTTCAACGGAACGACTGTCACCCGCCAAGCCTTGACGGCGTTTACGTTCGTTGAGGCGATCGCGAAAATGCCGTTTCAAATCGCGGCATTCAAATTCACGAATGTTATTCACACCGTGATTGAGTTGTTGATACTTAAATTGAAAATAATCGTAAGAAGACTGCTCGTCAAAAATCGTGCGCGCCGATGGTTTGCCCACAATc                                                                                                                                                                                                                     <  1:849452/235‑1 (MQ=255)                                  gtcTATCGTTTGTCGTTGCTTCTGCATTTGGTTTTCAACGGAACGACTGTCACCCGCCAAGCCTTGACGGCGTTTACGTTCGTTGAGGCGATCGCGAAAATGCCGTTTCAAATCGCGGCATTCAAATTCACGAATGTTATTCACACCGTGATTGAGTTGTTGATACTTAAATTGAAAATAATCGTAAGAAGACTGCTCGTCAAAAATCGTGCGCGCCGATGGTTTGCCCACAATCTTCGCCAACTCGTCAt                                                                                                                                                                                                     >  1:561636/1‑251 (MQ=255)                                  gtcTATCGTTTGTCGTTGCTTCTGCATTTGGTTTTCAACGGAACGACTGTCACCCGCCAAGCCTTGACGGCGTTTACGTTCGTTGAGGCGATCGCGAAAATGCCGTTTCAAATCGCGGCATTCAAATTCACGAATGTTATTCACACCGTGATTGAGTTGTTGATACTTAAATTGAAAATAATCGTAAGAAGACTGCTCGTCAAAAATCGTGCGCGCCGATGGTTTGCCCACAATCTTCGCCAACTCGTCAt                                                                                                                                                                                                     >  1:839783/1‑251 (MQ=255)                                     tATCGTTTGTCGTTGCTTCTGCATTTGGTTTTCAACGGAACGACTGTCACCCGCCAAGCCTTGACGGCGTTTACGTTCGTTGAGGCGATCGCGAAAATGCCGTTTCAAATCGCGGCATTCAAATTCACGAATGTTATTCACACCGTGATTGAGTTGTTGATACTTAAATTGAAAATAATCGTAAGAAGACTGCTCGTCAAAAATCGTGCGCGCCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGa                                                                                                                                                                                                  <  1:652731/251‑1 (MQ=255)                                      aTCGTTTGTCGTTGCTTCTGCATTTGGTTTTCAACGGAACGACTGTCACCCGCCAAGCCTTGACGGCGTTTACGTTCGTTGAGGCGATCGCGAAAATGCCGTTTCAAATCGCGGCATTCAAATTCACGAATGTTATTCACACCGTGATTGAGTTGTTGATACTTAAATTGAAAATAATCGTAAGAAGACTGCTCGTCAAAAATCGTGCGCGCCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGAt                                                                                                                                                                                                 <  1:796636/251‑1 (MQ=255)                                       tCGTTTGTCGTTGCTTCTGCATTTGGTTTTCAACGGAACGACTGTCACCCGCCAAGCCTTGACGGCGTTTACGTTCGTTGAGGCGATCGCGAAAATGCCGTTTCAAATCGCGGCATTCAAATTCACGAATGTTATTCACACCGTGATTGAGTTGTTGATACTTAAATTGAAAATAATCGTAAGAAGACTGCTCGTCAAAAATCGTGCGCGCCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATc                                                                                                                                                                                                <  1:839317/251‑1 (MQ=255)                                       tCGTTTGTCGTTGCTTCTGCATTTGGTTTTCAACGGAACGACTGTCACCCGCCAAGCCTTGACGGCGTTTACGTTCGTTGAGGCGATCGCGAAAATGCCGTTTCAAATCGCGGCATTCAAATTCACGAATGTTATTCACACCGTGATTGAGTTGTTGATACTTAAATTGAAAATAATCGTAAGAAGACTGCTCGTCAAAAATCGTGCGCGCCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATc                                                                                                                                                                                                <  1:797376/251‑1 (MQ=255)                                           ttGTCGTTGCTTCTGCATTTGGTTTTCAACGGAACGACTGTCACCCGCCAAGCCTTGACGGCGTTTACGTTCGTTGAGGCGATCGCGAAAATGCCGTTTCAAATCGCGGCATTCAAATTCACGAATGTTATTCACACCGTGATTGAGTTGTTGATACTTAAATTGAAAATAATCGTAAGAAGACTGCTCGTCAAAAATCGTGCGCGCCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCatat                                                                                                                                                                                            >  1:634074/1‑251 (MQ=255)                                            tGTCGTTGCTTCTGCATTTGGTTTTCAACGGAACGACTGTCACCCGCCAAGCCTTGACGGCGTTTACGTTCGTTGAGGCGATCGCGAAAATGCCGTTTCAAATCGCGGCATTCAAATTCACGAATGTTATTCACACCGTGATTGAGTTGTTGATACTTAAATTGAAAATAATCGTAAGAAGACTGCTCGTCAAAAATCGTGCGCGCCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATc                                                                                                                                                                                           <  1:14135/251‑1 (MQ=255)                                                              tGGTTTTCAACGGAACGACTGTCACCCGCCAAGCCTTGACGGCGTTTACGTTCGTTGAGGCGATCGCGAAAATGCCGTTTCAAATCGCGGCATTCAAATTCACGAATGTTATTCACACCGTGATTGAGTTGTTGATACTTAAATTGAAAATAATCGTAAGAAGACTGCTCGTCAAAAATCGTGCGCGCCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCatat                                                                                                                                                                                            >  1:90385/1‑232 (MQ=255)                                                                gTTTTCAACGGAACGACTGTCACCCGCCAAGCCTTGACGGCGTTTACGTTCGTTGAGGCGATCGCGAAAATGCCGTTTCAAATCGCGGCATTCAAATTCACGAATGTTATTCACACCGTGATTGAGTTGTTGATACTTAAATTGAAAATAATCGTAAGAAGACTGCTCGTCAAAAATCGTGCGCGCCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCaa                                                                                                                                                                       >  1:826907/1‑251 (MQ=255)                                                                    tCAACGGAACGACTGTCACCCGCCAAGCCTTGACGGCGTTTACGTTCGTTGAGGCGATCGCGAAAATGCCGTTTCAAATCGCGGCATTCAAATTCACGAATGTTATTCACACCGTGATTGAGTTGTTGATACTTAAATTGAAAATAATCGTAAGAAGACTGCTCGTCAAAAATCGTGCGCGCCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGc                                                                                                                                                                   <  1:350079/251‑1 (MQ=255)                                                                            aCGACTGTCACCCGCCAAGCCTTGACGGCGTTTACGTTCGTTGAGGCGATCGCGAAAATGCCGTTTCAAATCGCGGCATTCAAATTCACGAATGTTATTCACACCGTGATTGAGTTGTTGATACTTAAATTGAAAATAATCGTAAGAAGACTGCTCGTCAAAAATCGTGCGCGCCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAacac                                                                                                                                                           <  1:562384/251‑1 (MQ=255)                                                                               aCTGTCCCCCGCCAAGCCTTGACGGCGTTTACGTTCGTTGAGGCGATCGCGAAAATGCCGTTTCAAATCGCGGCATTCAAATTCACGAATGTTATTCACACCGTGATTGAGTTGTTGATACTTAAATTGAAAATAATCGTAAGAAGACTGCTCGTCAAAAATCGTGCGCGCCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGt                                                                                                                                                         <  1:558556/250‑1 (MQ=255)                                                                               aCTGTCACCCGCCAAGCCTTGACGGCGTTTACGTTCGTTGAGGCGATCGCGAAAATGCCGTTTCAAATCGCGGCATTCAAATTCACGAATGTTATTCACACCGTGATTGAGTTGTTGATACTTAAATTGAAAATAATCGTAAGAAGACTGCTCGTCAAAAATCGTGCGCGCCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGtt                                                                                                                                                        <  1:624029/251‑1 (MQ=255)                                                                               aCTGTCACCCGCCAAGCCTTGACGGCGTTTACGTTCGTTGAGGCGATCGCGAAAATGCCGTTTCAAATCGCGGCATTCAAATTCACGAATGTTATTCACACCGTGATTGAGTTGTTGATACTTAAATTGAAAATAATCGTAAGAAGACTGCTCGTCAAAAATCGTGCGCGCCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGt                                                                                                                                                         <  1:226126/250‑1 (MQ=255)                                                                                                       gCGTTTGCGTTCGTTGAGGCGATCGCGAAAATGCCGTTTCAAATCGCGGCATTCAAATTCACGAATGTTATTCATGCCGTGATTGAGTTGTTGATTCTTAAATTGAAAATGATCGTAAGAAGTCTGCACGTCAAAAATCATGCGCGCCGAAGTTTTGCCCACAATCTTCGCAAACTCGTCATTGATCATATCTTTTAATa                                                                                                                                                                                   <  1:27893/200‑1 (MQ=255)                                                                                                         gTTTACGTTCGTTGAGGCGATCGCGAAAATGCCGTTTCAAATCGCGGCATTCAAATTCACGAATGTTATTCACACCGTGATTGAGTTGTTGATACTTAAATTGAAAATAATCGTAAGAAGACTGCTCGTCAAAAATCGTGCGCGCCGATGGTTTGCCCACAATCTTCgcc                                                                                                                                                                                                               >  1:848133/1‑168 (MQ=255)                                                                                                                ttcgttGAGGCGATCGCGAAAATGCCGTTTCAAATCGCGGCATTCAAATTCACGAATGTTATTCACACCGTGATTGAGTTGTTGATACTTAAATTGAAAATAATCGTAAGAAGACTGCTCGTCAAAAATCGTGCGCGCCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCaacaaac                                                                                                                       <  1:347133/251‑1 (MQ=255)                                                                                                                 tcgttGAGGCGATCGCGAAAATGCCGTTTCAAATCGCGGCATTCAAATTCACGAATGTTATTCACACCGTGATTGAGTTGTTGATACTTAAATTGAAAATAATCGTAAGAAGACTGCTCGTCAAAAATCGTGCGCGCCGATGGTTTGccc                                                                                                                                                                                                                           <  1:863529/150‑1 (MQ=255)                                                                                                                      gaggcGATCGCGAAAATGCCGTTTCAAATCGCGGCATTCAAATTCACGAATGTTATTCACACCGTGATTGAGTTGTTGATACTTAAATTGAAAATAATCGTAAGAAGACTGCTCGTCAAAAATCGTGCGCGCCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGt                                                                                                                                                         >  1:707499/5‑211 (MQ=255)                                                                                                                        ggcGATCGCGAAAATGCCGTTTCAAATCGCGGCATTCAAATTCACGAATGTTATTCACACCGTGATTGAGTTGTTGATACTTAAATTGAAAATAATCGTAAGAAGACTGCTCGTCAAAAATCGTGCGCGCCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTc                                                                                                                                  >  1:827179/3‑232 (MQ=255)                                                                                                                            aTCGCGAAAATGCCGTTTCAAATCGCGGCATTCAAATTCACGAATGTTATTCACACCGTGATTGAGTTGTTGATACTTAAATTGAAAATAATCGTAAGAAGACTGCTCGTCAAAAATCGTGCGCGCCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGtt                                                                                                                                                        >  1:119193/1‑206 (MQ=255)                                                                                                                                cgAAAATGCCGTTTCAAATCGCGGCATTCAAATTCACGAATGTTATTCACACCGTGATTGAGTTGTTGATACTTAAATTGAAAATAATCGTAAGAAGACTGCTCGTCAAAAATCGTGCGCGCCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTAtt                                                                                                       >  1:254921/1‑251 (MQ=255)                                                                                                                                  aaaatGCCGTTTCAAATCGCGGCATTCAAATTCACGAATGTTATTCACACCGTGATTGAGTTGTTGATACTTAAATTGAAAATAATCGTAAGAAGACTGCTCGTCAAAAATCGTGCGCGCCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTAttt                                                                                                      <  1:156926/246‑1 (MQ=255)                                                                                                                                         cGTTTCAAATCGCGGCATTCAAATTCACGAATGTTATTCACACCGTGATTGAGTTGTTGATACTTAAATTGAAAATAATCGTAAGAAGACTGCTCGTCAAAAATCGTGCGCGCCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTaa                                                                                              >  1:49425/1‑251 (MQ=255)                                                                                                                                                       gCATTCAAATTCACGAATGTTATTCACACCGTGATTGAGTTGTTGATACTTAAATTGAAAATAATCGTAAGAAGACTGCTCGTCAAAAATCGTGCGCGCCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATa                                                                                                                                                                                   >  1:427334/1‑152 (MQ=255)                                                                                                                                                       gCATTCAAATTCACGAATGTTATTCACACCGTGATTGAGTTGTTGATACTTAAATTGAAAATAATCGTAAGAAGACTGCTCGTCAAAAATCGTGCGCGCCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGttcgtc                                                                                                                                                    <  1:239162/183‑5 (MQ=255)                                                                                                                                                       gCATTCAAATTCACGAATGTTATTCACACCGTGATTGAGTTGTTGATACTTAAATTGAAAATAATCGTAAGAAGACTGCTCGTCAAAAATCGTGCGCGCCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGt                                                                                                                                                         >  1:129612/1‑178 (MQ=255)                                                                                                                                                         aTTCAAATTCACGAATGTTATTCACACCGTGATTGAGTTGTTGATACTTAAATTGAAAATAATCGTAAGAAGACTGCTCGTCAAAAATCGTGCGCGCCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGt                                                                                                                                                         >  1:421098/1‑176 (MQ=255)                                                                                                                                                                       aTGTTATTCACACCGTGATTGAGTTGTTGATACTTAAATTGAAAATAATCGTAAGAAGACTGCTCGTCAAAAATCGTGCGCGCCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCat                                                                                                                                                                                              <  1:465626/125‑1 (MQ=255)                                                                                                                                                                        tGTTATTCACACCGTGATTGAGTTGTTGATACTTAAATTGAAAATAATCGTAAGAAGACTGCTCGTCAAAAATCGTGCGCGCCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAAtt                                                               >  1:306521/1‑251 (MQ=255)                                                                                                                                                                         gTTATTCACACCGTGATTGAGTTGTTGATACTTAAATTGAAAATAATCGTAAGAAGACTGCTCGTCAAAAATCGTGCGCGCCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAggg                                                                                                                                                                    >  1:63035/1‑149 (MQ=255)                                                                                                                                                                          ttATTCACACCGTGATTGAGTTGTTGATACTTAAATTGAAAATAATCGTAAGAAGACTGCTCGTCAAAAATCGTGCGCGCCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAAtttt                                                             >  1:823312/1‑251 (MQ=255)                                                                                                                                                                                     gTGATTGAGTTGTTGATACTTAAATTGAAAATAATCGTAAGAAGACTGCTCGTCAAAAATCGTGCGCGCCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGa                                                                                                                                                                                                  >  1:422230/1‑107 (MQ=255)                                                                                                                                                                                     gTGATTGAGTTGTTGATACTTAAATTGAAAATAATCGTAAGAAGACTGCTCGTCAAAAATCGTGCGCGCCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGAt                                                  >  1:676342/1‑251 (MQ=255)                                                                                                                                                                                              ttgttgATACTTAAATTGAAAATAATCGTAAGAAGACTGCTCGTCAAAAATCGTGCGCGCCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGatat                                          <  1:610534/250‑1 (MQ=255)                                                                                                                                                                                                          aaaTTGAAAATAATCGTAAGAAGACTGCTCGTCAAAAATCGTGCGCGCCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCAtgcg                             <  1:195905/251‑1 (MQ=255)                                                                                                                                                                                                          aaaTTGAAAATAATCGTAAGAAGACTGCTCGTCAAAAATCGTGCGCGCCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCAtgcg                             <  1:869396/251‑1 (MQ=255)                                                                                                                                                                                                                        cGTAAGAAGACTGCTCGTCAAAAATCGTGCGCGCCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTcc                                                                                                               >  1:597034/1‑155 (MQ=255)                                                                                                                                                                                                                          tAAGAAGACTGCTCGTCAAAAATCGTGCGCGCCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAAtt                                                               >  1:623237/1‑201 (MQ=255)                                                                                                                                                                                                                          tAAGAAGACTGCTCGTCAAAAATCGTGCGCGCCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCAtgcg                             <  1:536522/235‑1 (MQ=255)                                                                                                                                                                                                                          tAAGAAGACTGCTCGTCAAAAATCGTGCGCGCCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGcggcgggtg                  <  1:816875/246‑4 (MQ=255)                                                                                                                                                                                                                           aagaagACTGCTCGTCAAAAATCGTGCGCGCCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGaaa            <  1:78169/251‑1 (MQ=255)                                                                                                                                                                                                                             gaagaCTGCTCGTCAAAAATCGTGCGCGCCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGaaaa           >  1:22906/1‑250 (MQ=255)                                                                                                                                                                                                                              aagaCTGCTCGTCAAAAATCGTGCGCGCCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCTTGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGaaaaag         >  1:186546/1‑249 (MQ=255)                                                                                                                                                                                                                                  ctgctCGTCAAAAATCGTGCGCGCCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCAt     >  1:219933/5‑251 (MQ=255)                                                                                                                                                                                                                                       cGTCAAAAATCGTGCGCGCCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCGGCAACAAACAATATTCCAAGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCTGTGGAAATTTTGTATCTCGGATCCTGATATTTCGGTTCATGCGTGCGGCGGGTGCTGAAAACGGCAtttt  >  1:408953/1‑246 (MQ=255)                                                                                                                                                                                                                                            aaaaTCGTGCGCGCCGATGGTTTGCCCACAATCTTCGCCAACTCGTCATTGATCATATCTTTTAATAAGCGTTTTTCAAGGGCAGTAACACGTTCGTCCGATTGCTTGCCCAATTCCggca                                                                                                                             <  1:520153/121‑3 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                            TTCTCGTTGCGTGTGAAAACGGCGAAACGCGTCGTCTATCGTTTGTCGTTGCTTTTGCACTTGGTTTTCAACAGAACGACTGTCGCCCGCCAAGCCTTGCCGGCGTTTGCGTTCGTTGAGACGATCGCGAAAGTGCCGTTTCAAATCGCGGCATTCAAATTCACGAATGTTATTCACACCGTGATTGAGTTGTTGATACTTAAATTGAAAATAATCGTAAGAAGACTGATCGTCAAAAATCGTACGCGCCGACGCTTTGCCCACAATCTTCGACAACTCGTCATTGATCATATCTTTTAATAGCCGTTTTTCAAGGGCGGTAACACGTTTATCCGATTGCTTGCCCAATTCCGGTAACAAACAATATTCCAGGTTATTTTTGGCTAAAATGAATTTTTCCATGTCTTCCGTGGAAATTTTGTATCTTGGATCCTGATATTTCGGTTCATGCGTGCGGCGGATGCTGAAAACGGCATGTT  >  1/1476180‑1476658

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑