Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,476,106	A→G	M226T (ATG→ACG)	unknown ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,476,106	0	A	G	100.0%	106.1 / NA	39	M226T (ATG→ACG)	unknown	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/21);  total (18/21)

GGGCATCTAAATGAATGCCTTGGCGCCCATCATAAATGAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAATGAAGTTGAGCGGAAAAGTGCGGCAAAACACCGCCAATCCACACCGCACTTTTCAGGGCAGGAAAGTATTCCCATTGGCGCGCTAACGTGGCATCGTCGCCAACAAGGAGAATTAGCTGCCGCGCCATGCTTTACCAACCCCGCGAATACGGATCCATCCAGTCTACATTGATATTTTTATTGCTTTTATAGAGCGGTTCGCTTTCGCCGCGAATTTGGCGTTCAATAGATTCTCGTTGCGTGTGAAAACGGCGAAACGCGTCGTCTATCGTTTGTCGTTGCTTTTGCACTTGGTTTTCAACAGAACGACTGTCGCCCGCCAAGCCTTGCCGGCGTTTGCGTTCGTTGAGACGATCGCGAAAGTGCCGTTTCAAATCGCGGCATTCAAATTCACGAATGTTA  >  1/1475861‑1476351                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                      gagCATCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTAACTGCCGCGCCATAGTTTACCAACCCCGTGAATACGGatccgtcc                                                                                                                                                                                                                                                    <  1:28446/247‑1 (MQ=255)                    ctgacgcCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTAACTGCCGCGCCATAGTTTACCAACCCCGTGAATACGGATCCGTCCAATCTACAtt                                                                                                                                                                                                                                          <  1:558484/236‑1 (MQ=255)                    ctgacgcCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTAACTGCCGCGCCATAGTTTACCAACCCCGTGAATACGGATCCGTCCAATCTACATTAATATTTTTAt                                                                                                                                                                                                                               <  1:160657/247‑1 (MQ=255)                               catAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTAACTGCCGCGCCATAGTTTACCAACCCCGTGAATACGGATCCGTCCAATCTACATTAATATTTTTATTGCTTTTATag                                                                                                                                                                                                                    <  1:11930/251‑1 (MQ=255)                               catAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTAACTGCCGCGCCATAGTTTACCAACCCCGTGAATACGGATCCGTCCAATCTACATTAATATTTTTATTGCTTTTATag                                                                                                                                                                                                                    <  1:590757/251‑1 (MQ=255)                               catAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTAACTGCCGCGCCATAGTTTACCAACCCCGTGAATACGGATCCGTCCAATCTACATTAATATTTTTATTGCTTTTATag                                                                                                                                                                                                                    <  1:823761/251‑1 (MQ=255)                                ataaataaGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTAACTGCCGCGCCATAGTTTACCAACCCCGTGAATACGGATCCGTCCAATCTACATTAATATTTTTATTGCTTTTATag                                                                                                                                                                                                                    <  1:154465/250‑1 (MQ=255)                                 tAAATAAGCGCGAGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTAACTGCCGCGCCATAGTTTACCAACCCCGTGAATACGGATCCGTCCAATCTACATTAATATTTTTATTGCTTTTATagag                                                                                                                                                                                                                  <  1:467338/251‑1 (MQ=255)                                             gggAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTAACTGCCGCGCCATAGTTTACCAACCCCGTGAATACGGATCCGTCCAATCTACATTAata                                                                                                                                                                                                                                      <  1:577465/219‑1 (MQ=255)                                                                 aTTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTAACTGCCGCGCCATAGTTTACCAACCCCGTGAATACGGATCCGTCCAATCTACATTAATATTTTTATTGCTTTTATAGAGCGGTTCACTTTCGCCGCGAATTTGGCGTTCaa                                                                                                                                                                                  >  1:108532/1‑251 (MQ=255)                                                                            tGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTAACTGCCGCGCCATAGTTTACCAACCCCGTGAATACGGATCCGTCCAATCTACATTAATATTTTTATTGCTTTTATAGAGCGGTTCACTTTCGCCGCGAATTTGGCGTTCAATAGATTCTCGt                                                                                                                                                                       <  1:198051/251‑1 (MQ=255)                                                                              cTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTAACTGCCGCGCCATAGTTTACCAACCCCGTGAATACGGATCCGTCCAATCTACATTAATATTTTTATTGCTTTTATAGAGCGGTTCACTTTCGCCGCGAATTTGGCGTTCAATAGATTCTCGTTg                                                                                                                                                                     <  1:572754/251‑1 (MQ=255)                                                                                             ggctGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTAACTGCCGCGCCATAGTTTACCAACCCCGTGAATACGGATCCGTCCAATCTACATTAATATTTTTATTGCTTTTATAGAGCGGTTCACTTTCGCCGCGAATTTGGCGTTCAATAGATTCTCGTTGCGTGTGAAAACGGCg                                                                                                                                                      >  1:367699/4‑251 (MQ=255)                                                                                                           gtgcggtgaaaCGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTAACTGCCGCGCCATAGTTTACCAACCCCGTGAATACGGATCCGTCCAATCTACATTAATATTTTTATTGCTTTTATAGAGCGGTTCACTTTCGCCGCGAATTTGGCGTTCAATAGATTCTCGTTGCGT‑TGAAAAccaaatgc                                                                                                                                                   >  1:123204/9‑232 (MQ=255)                                                                                                                gtgaaaCGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTAACTGCCGCGCCATAGTTTACCAACCCCGTGAATACGGATCCGTCCAATCTACATTAATATTTTTATTGCTTTTATAGAGCGGTTCACTTTCGCCGCGAATTTGGCGTTCAATAGATTCTCGTTg                                                                                                                                                                     >  1:5892/4‑217 (MQ=255)                                                                                                                gtgaaaCGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTAACTGCCGCGCCATAGTTTACCAACCCCGTGAATACGGATCCGTCCAATCTACATTAATATTTTTATTGCTTTTATAGAGCGGTTCACTTTCGCCGCGAATTTGGCGTTCAATAGATTCTCGTTGCGTGTGAAAACGGCGAAACGCATCGTCTATCGt                                                                                                                                    <  1:889965/247‑1 (MQ=255)                                                                                                                        ccGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTAACTGCCGCGCCATAGTTTACCAACCCCGTGAATACGAATCCGTCCAATCTACATTAATATTTTTATTGCTTTTATAGAGCGGTTCACTTTCGCCGCGAATTTGGCGTTCAATAGATTCTCGTTGCGTGTGAAAACGGCGAAACGCATCGTCTATCGTTTGTCGTTg                                                                                                                           >  1:214952/1‑251 (MQ=255)                                                                                                                              aTCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTAACTGCCGCGCCATAGTTTACCAACCCCGTGAATACGGATCCGTCCAATCTACATTAATATTTTTATTGCTTTTATAGAGCGGTTCACTTTCGCCGCGAATTTGGCGTTCAATAGATTCTCGTTGCGTGTGAAAACGGCGAAACGCATCGTCTATCGTTTGTCGTTGCtt                                                                                                                        >  1:210647/1‑248 (MQ=255)                                                                                                                                  atacCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTAACTGCCGCGCCATAGTTTACCAACCCCGTGAATACGGATCCGTCCAATCTACATTAATATTTTTATTGCTTTTATAGAGCGGTTCACTTTCGCCGCGAATTTGGCGTTCAATAGATTCTCGTTGCGTGTGAAAACGGCGAAACGCATCGTCTATCGTTTGTCGTTGCTTCTGCatt                                                                                                                 >  1:53669/3‑249 (MQ=255)                                                                                                                                      cGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTAACTGCCGCGCCATAGTTTACCAACCCCGTGAATACGGATCCGTCCAATCTACATTAATATTTTTATTGCTTTTATAGAGCGGTTCACTTTCGCCGCGAATTTGGCGTTCAATAGATTCTCGTTGCGTGTGAAAACGGCGAAACGCATCGTCTATCGTTTGTCGTTGCTTCTGCATTTGGt                                                                                                             <  1:573032/251‑1 (MQ=255)                                                                                                                                        cACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTAACTGCCGCGCCATAGTTTACCAACCCCGTGAATACGGATCCGTCCAATCTACATTAATATTTTTATTGCTTTTATAGAGCGGTTCACTTTCGCCGCGAATTTGGCGTTCAATAGATTCTCGTTGCGTGTGAAAACGGCGAAACGCATCGTCTATCGTTTGTCGTTGCTTCTGCATTTGGtt                                                                                                            <  1:246518/250‑1 (MQ=255)                                                                                                                                                   gCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTAACTGCCGCGCCATAGTTTACCAACCCCGTGAATACGGATCCGTCCAATCTACATTAATATTTTTATTGCTTTTATAGAGCGGTTCACTTTCGCCGCGAATTTGGCGTTCAATAGATTCTCGTTGCGTGTGAAAACGGCGAAACGCATCGTCTATCGTTTGTCGTTGCTTCTGCATTTGGTTTTCAACGGAAc                                                                                                 <  1:486308/250‑1 (MQ=255)                                                                                                                                                        aaaatgttCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTAACTGCCGCGCCATAGTTTACCAACCCCGTGAATACGGATCCGTCCAATCTACATTAATATTTTTATTGCTTTTATAGAGCGGTTCACTTTCGCCGCGAATTTGGCGTTCAATAGATTCTCGTTGCGTGTGAAAACGGCGAAACGCATCGTCTATCGTTTGTCGTTGCTTCTGCATTTGGTTTTCAACGGAACGa                                                                                               >  1:444568/7‑247 (MQ=255)                                                                                                                                                                          cgcTAACGTGGCATCGTCACCAATGAGAAGAATTAACTGCCGCGCCATAGTTTACCAACCCCGTGAATACGGATCCGTCCAATCTACATTAATATTTTTATTGCTTTTATAGAGCGGTTCACTTTCGCCGCGAATTTGGCGTTCAATAGATTCTCGTTGCGTGTGAAAACGGCGAAACGCATCGTCTATCGTTTGTCGTTGCTTCTGCATTTGGTTTTCAACGGAACGACTGTCACCCGCCAAGCCTTgac                                                                         <  1:709852/251‑3 (MQ=255)                                                                                                                                                                              aaCGTGGCATCGTCACCAATGAGAAGAATTAACTGCCGCGCCATAGTTTACCAACCCCGTGAATACGGATCCGTCCAATCTACATTAATATTTTTATTGCTTTTATAGAGCGGTTCACTTTCGCCGCGAATTTGGCGTTCAATAGATTCTCGTTGCGTGTGAAAACGGCGAAACGCATCGTCTATCGTTTGTCGTTGCTTCTGCATTTGGTTTTCAACGGAACGACTGTCACCCGCCAAGCCTTGACGGCg                                                                     >  1:42654/1‑251 (MQ=255)                                                                                                                                                                                      atcgtcaccaatgagaagaATTAACTGCCGCGCCATAGTTTACCAACCCCGTGAATACGGATCCGTCCAATCTACATTAATATTTTTATTGCTTTTATAGAGCGGTTCACTTTCGCCGCGAATTTGGCGTTCAATAGATTCTCGTTGCGTGTGAAAACGGCGAAACGCATCGTCTATCGTTTGTCGTTGCTTCTGCATTTGGTTTTCAACGGAACGACTGTCACCCGCCAAGCCTTGACGGCGTTTAcgtt                                                             <  1:259635/235‑1 (MQ=255)                                                                                                                                                                                          tcaccaatgagaagaATTAACTGCCGCGCCATAGTTTACCAACCCCGTGAATACGGATCCGTCCAATCTACATTAATATTTTTATTGCTTTTATAGAGCGGTTCACTTTCGCCGCGAATTTGGCGTTCAATAGAtt                                                                                                                                                                            <  1:164543/124‑1 (MQ=255)                                                                                                                                                                                          tcaccaatgagaagaATTAACTGCCGCGCCATAGTTTACCAACCCCGTGAATACGGATCCGTCCAATCTACATTAATATTTTTATTGCTTTTATAGAGCGGTTCACTTTCGCCGCGAATTTGGCGTTCAATAGATTCTCGTTGCGTGTGAAAACGGCGAAACGCATCGTCTATCGTTTGTCGTTGCTTCTGCATTTGGTTTTCAACGGAACGACTGTCACCCGCCAAGCCTTGACGGCGTTTAcgttcgtt                                                         >  1:585950/13‑251 (MQ=255)                                                                                                                                                                                                    gaagaATTAACTGCCGCGCCATAGTTTACCAACCCCGTGAATACGGATCCGTCCAATCTACATTAATATTTTTATTGCTTTTATAGAGCGGTTCACTTTCGCCGCGAATTTGGCGTTCAATAGATTCTCGTTGCGTGTGAAAACGGCGAAACGCATCGTCTATCGTTTGTCGTTGCTTCTGCATTTGGTTTTCAACGGAACGACTGTcacc                                                                                       >  1:88790/3‑208 (MQ=255)                                                                                                                                                                                                                       catagtttACCACCCCCGTGAATACGGATCCGTCCAATCTACATTAATATTTTTATTGCTTTTATAGAGCGGTTCACTTTCGCCGCGAATTTGGCGTTCAATAGATTCTCGTTGCGTGTGAAAACGGCGAAACGCATCGTCTATCGTTTGTCGTTGCTTCTGCATTTGGTTTTCAACGGAACGACTGTCCCCCGCCAAGCCTTGACGGCGTTTACGTTCGTTGAGGCGATCGCGAAAATGCCGTTTCAAAt                            <  1:61574/246‑1 (MQ=255)                                                                                                                                                                                                                       catagtttACCAACCCCGTGAATACGGATCCGTCCAATCTACATTAATATTTTTATTGCTTTTATAGAGCGGTTCACTTTCGCCGCGAATTTGGCGTTCAATAGATTCTCGTTGCGTGTGAAAACGGCGAAACGCATCGTCTATCGTTTGTCGTTGCTTCTGCATTTGGTTTTCAACGGAACGACTGTCACCCGCCAAGCCTTGACGGCGTTTACGTTCGTTGAGGCGATCGCGAAAATGCCGTTTCAAAt                            <  1:783882/246‑1 (MQ=255)                                                                                                                                                                                                                        atagtttACCAACCCCGTGAATACGGATCCGTCCAATCTACATTAATATTTTTATTGCTTTTATAGAGCGGTTCACTTTCGCCGCGAATTTGGCGTTCAATAGATTCTCGTTGCGTGTGAAAACGGCGAAACGCATCGTCTATCGTTTGTCGTTGCTTCTGCATTTGGTTTTCAACGGAACGACTGTCACCCGCCAAGCCTTGACGGCGTTTACGTTCGTTGAGGCGATCGCGAAAATGCCg                                    >  1:285007/5‑242 (MQ=255)                                                                                                                                                                                                                         tagtttACCAACCCCGTGAATACGGATCCGTCCAATCTACATTAATATTTTTATTGCTTTTATAGAGCGGTTCACTTTCGCCGCGAATTTGGCGTTCAATAGATTCTCGTTGCGTGTGAAAACGGCGAAACGCATCGTCTATCGTTTGTCGTTGCTTCTGCATTTGGTTTTCAACGGAACGACTGTCACCCGCCAAGCCTTGACGGCGtttacg                                                               >  1:42119/4‑211 (MQ=255)                                                                                                                                                                                                                           gtttACCAACCCCGTGAATACGGATCCGTCCAATCTACATTAATATTTTTATTGCTTTTATAGAGCGGTTCACTTTCGCCGCGAATTTGGCGTTCAATAGATTCTCGTTGCGTGTGAAAACGGCGAAACGCATCGTCTATCGTTTGTCGTTGCTTCTGCATTTGGTTTTCAACGGAACGACTGTCACCCGCCAAGCCTTGACGGCGTTTACGTTCGTTGAGGCGATCGCGAAAATGCCGTTTCAAATcgcg                        >  1:795635/2‑251 (MQ=255)                                                                                                                                                                                                                               aCCAACCCCGTGAATACGGATCCGTCCAATCTACATTAATATTTTTATTGCTTTTATAGAGCGGTTCACTTTCGCCGCGAATTTGGCGTTCAATAGATTCTCGTTGCGTGTGAAAACGGCGAAACGCATCGTCTATCGTTTGTCGTTGCTTCTGCATTTGGTTTTCAACGGAACGACTGTCACCCGCCAAGCCTTGACGGCGTTTACGTTCGTTGAGGCGATCGCGAAAATGCCGTTTCAAATCGCGGCAt                    >  1:898787/1‑251 (MQ=255)                                                                                                                                                                                                                                       cgtgAATACGGATCCGTCCAATCTACATTAATATTTTTATTGCTTTTATAGAGCGGTTCACTTTCGCCGCGAATTTGGCGTTCAATAGATTCTCGTTGCGTGTGAAAACGGCGAAACGCATCGTCTATCGTTTGTCGTTGCTTCTGCATTTGGTTTTCAACGGAACGACTGTCACCCGCCAAGCCTTGACGGCGTTTACGTTCGTTGAGGCGATCGCGAAAATGCCGTTTCAAATCGCGGCATTCAAAtt             >  1:106947/4‑250 (MQ=255)                                                                                                                                                                                                                                        gtgAATACGGATCCGTCCAATCTACATTAATATTTTTATTGCTTTTATAGAGCGGTTCACTTTCGCCGCGAATTTGGCGTTCAATAGATTCTCGTTGCGTGTGAAAACGGCGAAACGCATCGTCTATCGTTTGTCGTTGCTTCTGCATTTGGTTTTCAACGGAACGACTGTCACCCGCCAAGCCTTGACGGCGTTTACGTTCGTTGAGGCGATCGCGAAAATGCCGTTTCAAATCGCGGCATTCAAATTCa           >  1:508193/3‑251 (MQ=255)                                                                                                                                                                                                                                          gAATACGGATCCGTCCAATCTGCATTAATATTTTTATTGCTTTTATAGAGCGGTTCACTTTCGCCGCGAATTTGGCGTTCAATAGATTCTCGTTGCGTGTGAAAACGGCGAAACGCATCGTCTATCGTTTGTCGTTGCTTCTGCATTTGGTTTTCAACGGAACGACTGTCACCCGCCAAGCCTTGACGGCGTTTACGTTCGTTGAGGCGATCGCGAAAATGCCGTTTCAAAt                            <  1:894766/232‑1 (MQ=255)                                                                                                                                                                                                                                                 gATCCGTCCAATCTACATTAATATTTTTATTGCTTTTATAGAGCGGTTCACTTTCGCCGCGAATTTGGCGTTCAATAGATTCTCGTTGCGTGTGAAAACGGCGAAACGCATCGTCTATCGt                                                                                                                                    <  1:342901/121‑1 (MQ=255)                                                                                                                                                                                                                                                 gATCCGTCCAATCTACATTAATATTTTTATTGCTTTTATAGAGCGGTTCACTTTCGCCGCGAATTTGGCGTTCAATAGATTCTCGTTGCGTGTGAAAACGGCGAAACGCATCGTCTATCGTTTGTCGTTGCTTCTGCATTTGGTTTTCAACGGAACGACTGTCACCCGCCAAGCCTTGACGGCGTTTACGTTCGTTGAGGCGATCGCGAAAATGCCGTTTCAAATCGCGGCATTCAAATTCACGAATGTTa  >  1:863042/1‑251 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                      GGGCATCTAAATGAATGCCTTGGCGCCCATCATAAATGAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAATGAAGTTGAGCGGAAAAGTGCGGCAAAACACCGCCAATCCACACCGCACTTTTCAGGGCAGGAAAGTATTCCCATTGGCGCGCTAACGTGGCATCGTCGCCAACAAGGAGAATTAGCTGCCGCGCCATGCTTTACCAACCCCGCGAATACGGATCCATCCAGTCTACATTGATATTTTTATTGCTTTTATAGAGCGGTTCGCTTTCGCCGCGAATTTGGCGTTCAATAGATTCTCGTTGCGTGTGAAAACGGCGAAACGCGTCGTCTATCGTTTGTCGTTGCTTTTGCACTTGGTTTTCAACAGAACGACTGTCGCCCGCCAAGCCTTGCCGGCGTTTGCGTTCGTTGAGACGATCGCGAAAGTGCCGTTTCAAATCGCGGCATTCAAATTCACGAATGTTA  >  1/1475861‑1476351

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑