Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,476,093	C→T	S230S (TCG→TCA)	unknown ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,476,093	0	C	T	100.0%	109.4 / NA	41	S230S (TCG→TCA)	unknown	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (16/25);  total (16/25)

CAATAGCGAGGGCATCTAAATGAATGCCTTGGCGCCCATCATAAATGAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAATGAAGTTGAGCGGAAAAGTGCGGCAAAACACCGCCAATCCACACCGCACTTTTCAGGGCAGGAAAGTATTCCCATTGGCGCGCTAACGTGGCATCGTCGCCAACAAGGAGAATTAGCTGCCGCGCCATGCTTTACCAACCCCGCGAATACGGATCCATCCAGTCTACATTGATATTTTTATTGCTTTTATAGAGCGGTTCGCTTTCGCCGCGAATTTGGCGTTCAATAGATTCTCGTTGCGTGTGAAAACGGCGAAACGCGTCGTCTATCGTTTGTCGTTGCTTTTGCACTTGGTTTTCAACAGAACGACTGTCGCCCGCCAAGCCTTGCCGGCGTTTGCGTTCGTTGAGACGATCGCGAAAGTGCCGTTTCAAATCGCGGCAT  >  1/1475852‑1476333                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                 cAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTAACTGCCGCGCCATAGTTTACCAACCCCGTGAATACGGa                                                                                                                                                                                                                                         <  1:179552/251‑1 (MQ=255)   atggCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTAACTGCCGCGCCATAGTTTACCAACCCCGTGAATACGGatc                                                                                                                                                                                                                                       <  1:238826/248‑1 (MQ=255)          gagCATCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTAACTGCCGCGCCATAGTTTACCAACCCCGTGAATACGGatccgtcc                                                                                                                                                                                                                                  <  1:28446/247‑1 (MQ=255)             cATCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTAACTGCCGCGCCATAGTTTACCAACCCCGTGAATACGGatc                                                                                                                                                                                                                                       <  1:301001/241‑1 (MQ=255)                             ctgacgcCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTAACTGCCGCGCCATAGTTTACCAACCCCGTGAATACGGATCCGTCCAATCTACAtt                                                                                                                                                                                                                        <  1:558484/236‑1 (MQ=255)                             ctgacgcCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTAACTGCCGCGCCATAGTTTACCAACCCCGTGAATACGGATCCGTCCAATCTACATTAATATTTTTAt                                                                                                                                                                                                             <  1:160657/247‑1 (MQ=255)                                       tcatAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTAACTGCCGCGCCATAGTTTACCAACCCCGTGAATACGGatc                                                                                                                                                                                                                                       >  1:373470/1‑215 (MQ=255)                                       tcatAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACCTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTAACTGCCGCGCCATAGTTTACCAACCCCGTGAATACGGa                                                                                                                                                                                                                                         <  1:437524/213‑1 (MQ=255)                                        catAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTAACTGCCGCGCCATAGTTTACCAACCCCGTGAATACGGATCCGTCCAATCTACATTAATATTTTTATTGCTTTTATag                                                                                                                                                                                                  <  1:11930/251‑1 (MQ=255)                                        catAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTAACTGCCGCGCCATAGTTTACCAACCCCGTGAATACGGATCCGTCCAATCTACATTAATATTTTTATTGCTTTTATag                                                                                                                                                                                                  <  1:823761/251‑1 (MQ=255)                                        catAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTAACTGCCGCGCCATAGTTTACCAACCCCGTGAATACGGATCCGTCCAATCTACATTAATATTTTTATTGCTTTTATag                                                                                                                                                                                                  <  1:590757/251‑1 (MQ=255)                                         ataaataaGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTAACTGCCGCGCCATAGTTTACCAACCCCGTGAATACGGATCCGTCCAATCTACATTAATATTTTTATTGCTTTTATag                                                                                                                                                                                                  <  1:154465/250‑1 (MQ=255)                                          tAAATAAGCGCGAGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTAACTGCCGCGCCATAGTTTACCAACCCCGTGAATACGGATCCGTCCAATCTACATTAATATTTTTATTGCTTTTATagag                                                                                                                                                                                                <  1:467338/251‑1 (MQ=255)                                                      gggAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTAACTGCCGCGCCATAGTTTACCAACCCCGTGAATACGGATCCGTCCAATCTACATTAata                                                                                                                                                                                                                    <  1:577465/219‑1 (MQ=255)                                                        gAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTAACTGCCGCGCCATAGTTTACCAACCCCGTGAATACGGa                                                                                                                                                                                                                                         <  1:647836/196‑1 (MQ=255)                                                                          aTTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTAACTGCCGCGCCATAGTTTACCAACCCCGTGAATACGGATCCGTCCAATCTACATTAATATTTTTATTGCTTTTATAGAGCGGTTCACTTTCGCCGCGAATTTGGCGTTCaa                                                                                                                                                                >  1:108532/1‑251 (MQ=255)                                                                                     tGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTAACTGCCGCGCCATAGTTTACCAACCCCGTGAATACGGATCCGTCCAATCTACATTAATATTTTTATTGCTTTTATAGAGCGGTTCACTTTCGCCGCGAATTTGGCGTTCAATAGATTCTCGt                                                                                                                                                     <  1:198051/251‑1 (MQ=255)                                                                                       cTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTAACTGCCGCGCCATAGTTTACCAACCCCGTGAATACGGATCCGTCCAATCTACATTAATATTTTTATTGCTTTTATAGAGCGGTTCACTTTCGCCGCGAATTTGGCGTTCAATAGATTCTCGTTg                                                                                                                                                   <  1:572754/251‑1 (MQ=255)                                                                                                      ggctGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTAACTGCCGCGCCATAGTTTACCAACCCCGTGAATACGGATCCGTCCAATCTACATTAATATTTTTATTGCTTTTATAGAGCGGTTCACTTTCGCCGCGAATTTGGCGTTCAATAGATTCTCGTTGCGTGTGAAAACGGCg                                                                                                                                    >  1:367699/4‑251 (MQ=255)                                                                                                                    gtgcggtgaaaCGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTAACTGCCGCGCCATAGTTTACCAACCCCGTGAATACGGATCCGTCCAATCTACATTAATATTTTTATTGCTTTTATAGAGCGGTTCACTTTCGCCGCGAATTTGGCGTTCAATAGATTCTCGTTGCGT‑TGAAAAccaaatgc                                                                                                                                 >  1:123204/9‑232 (MQ=255)                                                                                                                         gtgaaaCGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTAACTGCCGCGCCATAGTTTACCAACCCCGTGAATACGGATCCGTCCAATCTACATTAATATTTTTATTGCTTTTATAGAGCGGTTCACTTTCGCCGCGAATTTGGCGTTCAATAGATTCTCGTTg                                                                                                                                                   >  1:5892/4‑217 (MQ=255)                                                                                                                         gtgaaaCGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTAACTGCCGCGCCATAGTTTACCAACCCCGTGAATACGGATCCGTCCAATCTACATTAATATTTTTATTGCTTTTATAGAGCGGTTCACTTTCGCCGCGAATTTGGCGTTCAATAGATTCTCGTTGCGTGTGAAAACGGCGAAACGCATCGTCTATCGt                                                                                                                  <  1:889965/247‑1 (MQ=255)                                                                                                                                 ccGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTAACTGCCGCGCCATAGTTTACCAACCCCGTGAATACGAATCCGTCCAATCTACATTAATATTTTTATTGCTTTTATAGAGCGGTTCACTTTCGCCGCGAATTTGGCGTTCAATAGATTCTCGTTGCGTGTGAAAACGGCGAAACGCATCGTCTATCGTTTGTCGTTg                                                                                                         >  1:214952/1‑251 (MQ=255)                                                                                                                                       aTCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTAACTGCCGCGCCATAGTTTACCAACCCCGTGAATACGGATCCGTCCAATCTACATTAATATTTTTATTGCTTTTATAGAGCGGTTCACTTTCGCCGCGAATTTGGCGTTCAATAGATTCTCGTTGCGTGTGAAAACGGCGAAACGCATCGTCTATCGTTTGTCGTTGCtt                                                                                                      >  1:210647/1‑248 (MQ=255)                                                                                                                                           atacCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTAACTGCCGCGCCATAGTTTACCAACCCCGTGAATACGGATCCGTCCAATCTACATTAATATTTTTATTGCTTTTATAGAGCGGTTCACTTTCGCCGCGAATTTGGCGTTCAATAGATTCTCGTTGCGTGTGAAAACGGCGAAACGCATCGTCTATCGTTTGTCGTTGCTTCTGCatt                                                                                               >  1:53669/3‑249 (MQ=255)                                                                                                                                               cGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTAACTGCCGCGCCATAGTTTACCAACCCCGTGAATACGGATCCGTCCAATCTACATTAATATTTTTATTGCTTTTATAGAGCGGTTCACTTTCGCCGCGAATTTGGCGTTCAATAGATTCTCGTTGCGTGTGAAAACGGCGAAACGCATCGTCTATCGTTTGTCGTTGCTTCTGCATTTGGt                                                                                           <  1:573032/251‑1 (MQ=255)                                                                                                                                                 cACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTAACTGCCGCGCCATAGTTTACCAACCCCGTGAATACGGATCCGTCCAATCTACATTAATATTTTTATTGCTTTTATAGAGCGGTTCACTTTCGCCGCGAATTTGGCGTTCAATAGATTCTCGTTGCGTGTGAAAACGGCGAAACGCATCGTCTATCGTTTGTCGTTGCTTCTGCATTTGGtt                                                                                          <  1:246518/250‑1 (MQ=255)                                                                                                                                                            gCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTAACTGCCGCGCCATAGTTTACCAACCCCGTGAATACGGATCCGTCCAATCTACATTAATATTTTTATTGCTTTTATAGAGCGGTTCACTTTCGCCGCGAATTTGGCGTTCAATAGATTCTCGTTGCGTGTGAAAACGGCGAAACGCATCGTCTATCGTTTGTCGTTGCTTCTGCATTTGGTTTTCAACGGAAc                                                                               <  1:486308/250‑1 (MQ=255)                                                                                                                                                                 aaaatgttCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTAACTGCCGCGCCATAGTTTACCAACCCCGTGAATACGGATCCGTCCAATCTACATTAATATTTTTATTGCTTTTATAGAGCGGTTCACTTTCGCCGCGAATTTGGCGTTCAATAGATTCTCGTTGCGTGTGAAAACGGCGAAACGCATCGTCTATCGTTTGTCGTTGCTTCTGCATTTGGTTTTCAACGGAACGa                                                                             >  1:444568/7‑247 (MQ=255)                                                                                                                                                                                   cgcTAACGTGGCATCGTCACCAATGAGAAGAATTAACTGCCGCGCCATAGTTTACCAACCCCGTGAATACGGATCCGTCCAATCTACATTAATATTTTTATTGCTTTTATAGAGCGGTTCACTTTCGCCGCGAATTTGGCGTTCAATAGATTCTCGTTGCGTGTGAAAACGGCGAAACGCATCGTCTATCGTTTGTCGTTGCTTCTGCATTTGGTTTTCAACGGAACGACTGTCACCCGCCAAGCCTTgac                                                       <  1:709852/251‑3 (MQ=255)                                                                                                                                                                                       aaCGTGGCATCGTCACCAATGAGAAGAATTAACTGCCGCGCCATAGTTTACCAACCCCGTGAATACGGATCCGTCCAATCTACATTAATATTTTTATTGCTTTTATAGAGCGGTTCACTTTCGCCGCGAATTTGGCGTTCAATAGATTCTCGTTGCGTGTGAAAACGGCGAAACGCATCGTCTATCGTTTGTCGTTGCTTCTGCATTTGGTTTTCAACGGAACGACTGTCACCCGCCAAGCCTTGACGGCg                                                   >  1:42654/1‑251 (MQ=255)                                                                                                                                                                                               atcgtcaccaatgagaagaATTAACTGCCGCGCCATAGTTTACCAACCCCGTGAATACGGATCCGTCCAATCTACATTAATATTTTTATTGCTTTTATAGAGCGGTTCACTTTCGCCGCGAATTTGGCGTTCAATAGATTCTCGTTGCGTGTGAAAACGGCGAAACGCATCGTCTATCGTTTGTCGTTGCTTCTGCATTTGGTTTTCAACGGAACGACTGTCACCCGCCAAGCCTTGACGGCGTTTAcgtt                                           <  1:259635/235‑1 (MQ=255)                                                                                                                                                                                                   tcaccaatgagaagaATTAACTGCCGCGCCATAGTTTACCAACCCCGTGAATACGGATCCGTCCAATCTACATTAATATTTTTATTGCTTTTATAGAGCGGTTCACTTTCGCCGCGAATTTGGCGTTCAATAGAtt                                                                                                                                                          <  1:164543/124‑1 (MQ=255)                                                                                                                                                                                                   tcaccaatgagaagaATTAACTGCCGCGCCATAGTTTACCAACCCCGTGAATACGGATCCGTCCAATCTACATTAATATTTTTATTGCTTTTATAGAGCGGTTCACTTTCGCCGCGAATTTGGCGTTCAATAGATTCTCGTTGCGTGTGAAAACGGCGAAACGCATCGTCTATCGTTTGTCGTTGCTTCTGCATTTGGTTTTCAACGGAACGACTGTCACCCGCCAAGCCTTGACGGCGTTTAcgttcgtt                                       >  1:585950/13‑251 (MQ=255)                                                                                                                                                                                                             gaagaATTAACTGCCGCGCCATAGTTTACCAACCCCGTGAATACGGATCCGTCCAATCTACATTAATATTTTTATTGCTTTTATAGAGCGGTTCACTTTCGCCGCGAATTTGGCGTTCAATAGATTCTCGTTGCGTGTGAAAACGGCGAAACGCATCGTCTATCGTTTGTCGTTGCTTCTGCATTTGGTTTTCAACGGAACGACTGTcacc                                                                     >  1:88790/3‑208 (MQ=255)                                                                                                                                                                                                                                catagtttACCACCCCCGTGAATACGGATCCGTCCAATCTACATTAATATTTTTATTGCTTTTATAGAGCGGTTCACTTTCGCCGCGAATTTGGCGTTCAATAGATTCTCGTTGCGTGTGAAAACGGCGAAACGCATCGTCTATCGTTTGTCGTTGCTTCTGCATTTGGTTTTCAACGGAACGACTGTCCCCCGCCAAGCCTTGACGGCGTTTACGTTCGTTGAGGCGATCGCGAAAATGCCGTTTCAAAt          <  1:61574/246‑1 (MQ=255)                                                                                                                                                                                                                                catagtttACCAACCCCGTGAATACGGATCCGTCCAATCTACATTAATATTTTTATTGCTTTTATAGAGCGGTTCACTTTCGCCGCGAATTTGGCGTTCAATAGATTCTCGTTGCGTGTGAAAACGGCGAAACGCATCGTCTATCGTTTGTCGTTGCTTCTGCATTTGGTTTTCAACGGAACGACTGTCACCCGCCAAGCCTTGACGGCGTTTACGTTCGTTGAGGCGATCGCGAAAATGCCGTTTCAAAt          <  1:783882/246‑1 (MQ=255)                                                                                                                                                                                                                                 atagtttACCAACCCCGTGAATACGGATCCGTCCAATCTACATTAATATTTTTATTGCTTTTATAGAGCGGTTCACTTTCGCCGCGAATTTGGCGTTCAATAGATTCTCGTTGCGTGTGAAAACGGCGAAACGCATCGTCTATCGTTTGTCGTTGCTTCTGCATTTGGTTTTCAACGGAACGACTGTCACCCGCCAAGCCTTGACGGCGTTTACGTTCGTTGAGGCGATCGCGAAAATGCCg                  >  1:285007/5‑242 (MQ=255)                                                                                                                                                                                                                                  tagtttACCAACCCCGTGAATACGGATCCGTCCAATCTACATTAATATTTTTATTGCTTTTATAGAGCGGTTCACTTTCGCCGCGAATTTGGCGTTCAATAGATTCTCGTTGCGTGTGAAAACGGCGAAACGCATCGTCTATCGTTTGTCGTTGCTTCTGCATTTGGTTTTCAACGGAACGACTGTCACCCGCCAAGCCTTGACGGCGtttacg                                             >  1:42119/4‑211 (MQ=255)                                                                                                                                                                                                                                    gtttACCAACCCCGTGAATACGGATCCGTCCAATCTACATTAATATTTTTATTGCTTTTATAGAGCGGTTCACTTTCGCCGCGAATTTGGCGTTCAATAGATTCTCGTTGCGTGTGAAAACGGCGAAACGCATCGTCTATCGTTTGTCGTTGCTTCTGCATTTGGTTTTCAACGGAACGACTGTCACCCGCCAAGCCTTGACGGCGTTTACGTTCGTTGAGGCGATCGCGAAAATGCCGTTTCAAATcgcg      >  1:795635/2‑251 (MQ=255)                                                                                                                                                                                                                                        aCCAACCCCGTGAATACGGATCCGTCCAATCTACATTAATATTTTTATTGCTTTTATAGAGCGGTTCACTTTCGCCGCGAATTTGGCGTTCAATAGATTCTCGTTGCGTGTGAAAACGGCGAAACGCATCGTCTATCGTTTGTCGTTGCTTCTGCATTTGGTTTTCAACGGAACGACTGTCACCCGCCAAGCCTTGACGGCGTTTACGTTCGTTGAGGCGATCGCGAAAATGCCGTTTCAAATCGCGGCAt  >  1:898787/1‑251 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                 CAATAGCGAGGGCATCTAAATGAATGCCTTGGCGCCCATCATAAATGAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAATGAAGTTGAGCGGAAAAGTGCGGCAAAACACCGCCAATCCACACCGCACTTTTCAGGGCAGGAAAGTATTCCCATTGGCGCGCTAACGTGGCATCGTCGCCAACAAGGAGAATTAGCTGCCGCGCCATGCTTTACCAACCCCGCGAATACGGATCCATCCAGTCTACATTGATATTTTTATTGCTTTTATAGAGCGGTTCGCTTTCGCCGCGAATTTGGCGTTCAATAGATTCTCGTTGCGTGTGAAAACGGCGAAACGCGTCGTCTATCGTTTGTCGTTGCTTTTGCACTTGGTTTTCAACAGAACGACTGTCGCCCGCCAAGCCTTGCCGGCGTTTGCGTTCGTTGAGACGATCGCGAAAGTGCCGTTTCAAATCGCGGCAT  >  1/1475852‑1476333

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑