Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,476,057	G→A	L7L (CTC→CTT)	unknown ←	tRNA cytosine(34) acetyltransferase (EC 2.3.1.193)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,476,057	0	G	A	100.0%	107.1 / NA	41	L7L (CTC→CTT)	unknown	tRNA cytosine(34) acetyltransferase (EC 2.3.1.193)
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (15/26);  total (15/26)

CAACAATGTGCCGCCTTCCGACAACGCACCGGCGGCAATAGCGAGGGCATCTAAATGAATGCCTTGGCGCCCATCATAAATGAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAATGAAGTTGAGCGGAAAAGTGCGGCAAAACACCGCCAATCCACACCGCACTTTTCAGGGCAGGAAAGTATTCCCATTGGCGCGCTAACGTGGCATCGTCGCCAACAAGGAGAATTAGCTGCCGCGCCATGCTTTACCAACCCCGCGAATACGGATCCATCCAGTCTACATTGATATTTTTATTGCTTTTATAGAGCGGTTCGCTTTCGCCGCGAATTTGGCGTTCAATAGATTCTCGTTGCGTGTGAAAACGGCGAAACGCGTCGTCTATCGTTTGTCGTTGCTTTTGCACTTGGTTTTCAACAGAACGACTGTCGCCCGCCAAGCCTTGCCGGCG  >  1/1475817‑1476284                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                    caacaaTATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTaact                                                                                                                                                                                                                           <  1:870287/251‑4 (MQ=255) caacaaTATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTaact                                                                                                                                                                                                                           <  1:470535/251‑4 (MQ=255) caacaaTATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTaa                                                                                                                                                                                                                             >  1:56150/1‑248 (MQ=255)   acaaTATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTaac                                                                                                                                                                                                                            >  1:271834/1‑246 (MQ=255)   acaaTATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTAACTg                                                                                                                                                                                                                          >  1:193059/1‑250 (MQ=255)   acaaTATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTAACTg                                                                                                                                                                                                                          >  1:300262/1‑250 (MQ=255)              ccTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCTGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTAACTGCCGCGCCAtag                                                                                                                                                                                                               <  1:212219/250‑3 (MQ=255)                ttCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTAACTGCCGCGCCAtagttt                                                                                                                                                                                                            >  1:248402/1‑246 (MQ=255)                                    cAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTAACTGCCGCGCCATAGTTTACCAACCCCGTGAATACGGa                                                                                                                                                                                        <  1:179552/251‑1 (MQ=255)                                      atggCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTAACTGCCGCGCCATAGTTTACCAACCCCGTGAATACGGatc                                                                                                                                                                                      <  1:238826/248‑1 (MQ=255)                                        ggCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTAACTGCCGCGCCAtagt                                                                                                                                                                                                              <  1:592869/224‑4 (MQ=255)                                             gagCATCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTAACTGCCGCGCCATAGTTTACCAACCCCGTGAATACGGatccgtcc                                                                                                                                                                                 <  1:28446/247‑1 (MQ=255)                                                cATCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTAACTGCCGCGCCATAGTTTACCAACCCCGTGAATACGGatc                                                                                                                                                                                      <  1:301001/241‑1 (MQ=255)                                                 aTCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTAACTGCCGCGCCAtagttt                                                                                                                                                                                                            <  1:492585/218‑6 (MQ=255)                                                                ctgacgcCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTAACTGCCGCGCCATAGTTTACCAACCCCGTGAATACGGATCCGTCCAATCTACAtt                                                                                                                                                                       <  1:558484/236‑1 (MQ=255)                                                                ctgacgcCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTAACTGCCGCGCCATAGTTTACCAACCCCGTGAATACGGATCCGTCCAATCTACATTAATATTTTTAt                                                                                                                                                            <  1:160657/247‑1 (MQ=255)                                                                          tcatAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTAACTGCCGCGCCATAGTTTACCAACCCCGTGAATACGGatc                                                                                                                                                                                      >  1:373470/1‑215 (MQ=255)                                                                          tcatAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACCTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTAACTGCCGCGCCATAGTTTACCAACCCCGTGAATACGGa                                                                                                                                                                                        <  1:437524/213‑1 (MQ=255)                                                                           catAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTAACTGCCGCGCCATAGTTTACCAACCCCGTGAATACGGATCCGTCCAATCTACATTAATATTTTTATTGCTTTTATag                                                                                                                                                 <  1:11930/251‑1 (MQ=255)                                                                           catAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTAACTGCCGCGCCATAGTTTACCAACCCCGTGAATACGGATCCGTCCAATCTACATTAATATTTTTATTGCTTTTATag                                                                                                                                                 <  1:590757/251‑1 (MQ=255)                                                                           catAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTAACTGCCGCGCCATAGTTTACCAACCCCGTGAATACGGATCCGTCCAATCTACATTAATATTTTTATTGCTTTTATag                                                                                                                                                 <  1:823761/251‑1 (MQ=255)                                                                            ataaataaGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTAACTGCCGCGCCATAGTTTACCAACCCCGTGAATACGGATCCGTCCAATCTACATTAATATTTTTATTGCTTTTATag                                                                                                                                                 <  1:154465/250‑1 (MQ=255)                                                                             tAAATAAGCGCGAGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTAACTGCCGCGCCATAGTTTACCAACCCCGTGAATACGGATCCGTCCAATCTACATTAATATTTTTATTGCTTTTATagag                                                                                                                                               <  1:467338/251‑1 (MQ=255)                                                                                         gggAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTAACTGCCGCGCCATAGTTTACCAACCCCGTGAATACGGATCCGTCCAATCTACATTAata                                                                                                                                                                   <  1:577465/219‑1 (MQ=255)                                                                                           gAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTAACTGCCGCGCCATAGTTTACCAACCCCGTGAATACGGa                                                                                                                                                                                        <  1:647836/196‑1 (MQ=255)                                                                                                             aTTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTAACTGCCGCGCCATAGTTTACCAACCCCGTGAATACGGATCCGTCCAATCTACATTAATATTTTTATTGCTTTTATAGAGCGGTTCACTTTCGCCGCGAATTTGGCGTTCaa                                                                                                               >  1:108532/1‑251 (MQ=255)                                                                                                                        tGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTAACTGCCGCGCCATAGTTTACCAACCCCGTGAATACGGATCCGTCCAATCTACATTAATATTTTTATTGCTTTTATAGAGCGGTTCACTTTCGCCGCGAATTTGGCGTTCAATAGATTCTCGt                                                                                                    <  1:198051/251‑1 (MQ=255)                                                                                                                          cTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTAACTGCCGCGCCATAGTTTACCAACCCCGTGAATACGGATCCGTCCAATCTACATTAATATTTTTATTGCTTTTATAGAGCGGTTCACTTTCGCCGCGAATTTGGCGTTCAATAGATTCTCGTTg                                                                                                  <  1:572754/251‑1 (MQ=255)                                                                                                                                         ggctGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTAACTGCCGCGCCATAGTTTACCAACCCCGTGAATACGGATCCGTCCAATCTACATTAATATTTTTATTGCTTTTATAGAGCGGTTCACTTTCGCCGCGAATTTGGCGTTCAATAGATTCTCGTTGCGTGTGAAAACGGCg                                                                                   >  1:367699/4‑251 (MQ=255)                                                                                                                                                       gtgcggtgaaaCGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTAACTGCCGCGCCATAGTTTACCAACCCCGTGAATACGGATCCGTCCAATCTACATTAATATTTTTATTGCTTTTATAGAGCGGTTCACTTTCGCCGCGAATTTGGCGTTCAATAGATTCTCGTTGCGT‑TGAAAAccaaatgc                                                                                >  1:123204/9‑232 (MQ=255)                                                                                                                                                            gtgaaaCGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTAACTGCCGCGCCATAGTTTACCAACCCCGTGAATACGGATCCGTCCAATCTACATTAATATTTTTATTGCTTTTATAGAGCGGTTCACTTTCGCCGCGAATTTGGCGTTCAATAGATTCTCGTTg                                                                                                  >  1:5892/4‑217 (MQ=255)                                                                                                                                                            gtgaaaCGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTAACTGCCGCGCCATAGTTTACCAACCCCGTGAATACGGATCCGTCCAATCTACATTAATATTTTTATTGCTTTTATAGAGCGGTTCACTTTCGCCGCGAATTTGGCGTTCAATAGATTCTCGTTGCGTGTGAAAACGGCGAAACGCATCGTCTATCGt                                                                 <  1:889965/247‑1 (MQ=255)                                                                                                                                                                    ccGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTAACTGCCGCGCCATAGTTTACCAACCCCGTGAATACGAATCCGTCCAATCTACATTAATATTTTTATTGCTTTTATAGAGCGGTTCACTTTCGCCGCGAATTTGGCGTTCAATAGATTCTCGTTGCGTGTGAAAACGGCGAAACGCATCGTCTATCGTTTGTCGTTg                                                        >  1:214952/1‑251 (MQ=255)                                                                                                                                                                          aTCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTAACTGCCGCGCCATAGTTTACCAACCCCGTGAATACGGATCCGTCCAATCTACATTAATATTTTTATTGCTTTTATAGAGCGGTTCACTTTCGCCGCGAATTTGGCGTTCAATAGATTCTCGTTGCGTGTGAAAACGGCGAAACGCATCGTCTATCGTTTGTCGTTGCtt                                                     >  1:210647/1‑248 (MQ=255)                                                                                                                                                                              atacCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTAACTGCCGCGCCATAGTTTACCAACCCCGTGAATACGGATCCGTCCAATCTACATTAATATTTTTATTGCTTTTATAGAGCGGTTCACTTTCGCCGCGAATTTGGCGTTCAATAGATTCTCGTTGCGTGTGAAAACGGCGAAACGCATCGTCTATCGTTTGTCGTTGCTTCTGCatt                                              >  1:53669/3‑249 (MQ=255)                                                                                                                                                                                  cGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTAACTGCCGCGCCATAGTTTACCAACCCCGTGAATACGGATCCGTCCAATCTACATTAATATTTTTATTGCTTTTATAGAGCGGTTCACTTTCGCCGCGAATTTGGCGTTCAATAGATTCTCGTTGCGTGTGAAAACGGCGAAACGCATCGTCTATCGTTTGTCGTTGCTTCTGCATTTGGt                                          <  1:573032/251‑1 (MQ=255)                                                                                                                                                                                    cACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTAACTGCCGCGCCATAGTTTACCAACCCCGTGAATACGGATCCGTCCAATCTACATTAATATTTTTATTGCTTTTATAGAGCGGTTCACTTTCGCCGCGAATTTGGCGTTCAATAGATTCTCGTTGCGTGTGAAAACGGCGAAACGCATCGTCTATCGTTTGTCGTTGCTTCTGCATTTGGtt                                         <  1:246518/250‑1 (MQ=255)                                                                                                                                                                                               gCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTAACTGCCGCGCCATAGTTTACCAACCCCGTGAATACGGATCCGTCCAATCTACATTAATATTTTTATTGCTTTTATAGAGCGGTTCACTTTCGCCGCGAATTTGGCGTTCAATAGATTCTCGTTGCGTGTGAAAACGGCGAAACGCATCGTCTATCGTTTGTCGTTGCTTCTGCATTTGGTTTTCAACGGAAc                              <  1:486308/250‑1 (MQ=255)                                                                                                                                                                                                    aaaatgttCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTAACTGCCGCGCCATAGTTTACCAACCCCGTGAATACGGATCCGTCCAATCTACATTAATATTTTTATTGCTTTTATAGAGCGGTTCACTTTCGCCGCGAATTTGGCGTTCAATAGATTCTCGTTGCGTGTGAAAACGGCGAAACGCATCGTCTATCGTTTGTCGTTGCTTCTGCATTTGGTTTTCAACGGAACGa                            >  1:444568/7‑247 (MQ=255)                                                                                                                                                                                                                      cgcTAACGTGGCATCGTCACCAATGAGAAGAATTAACTGCCGCGCCATAGTTTACCAACCCCGTGAATACGGATCCGTCCAATCTACATTAATATTTTTATTGCTTTTATAGAGCGGTTCACTTTCGCCGCGAATTTGGCGTTCAATAGATTCTCGTTGCGTGTGAAAACGGCGAAACGCATCGTCTATCGTTTGTCGTTGCTTCTGCATTTGGTTTTCAACGGAACGACTGTCACCCGCCAAGCCTTgac      <  1:709852/251‑3 (MQ=255)                                                                                                                                                                                                                          aaCGTGGCATCGTCACCAATGAGAAGAATTAACTGCCGCGCCATAGTTTACCAACCCCGTGAATACGGATCCGTCCAATCTACATTAATATTTTTATTGCTTTTATAGAGCGGTTCACTTTCGCCGCGAATTTGGCGTTCAATAGATTCTCGTTGCGTGTGAAAACGGCGAAACGCATCGTCTATCGTTTGTCGTTGCTTCTGCATTTGGTTTTCAACGGAACGACTGTCACCCGCCAAGCCTTGACGGCg  >  1:42654/1‑251 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                    CAACAATGTGCCGCCTTCCGACAACGCACCGGCGGCAATAGCGAGGGCATCTAAATGAATGCCTTGGCGCCCATCATAAATGAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAATGAAGTTGAGCGGAAAAGTGCGGCAAAACACCGCCAATCCACACCGCACTTTTCAGGGCAGGAAAGTATTCCCATTGGCGCGCTAACGTGGCATCGTCGCCAACAAGGAGAATTAGCTGCCGCGCCATGCTTTACCAACCCCGCGAATACGGATCCATCCAGTCTACATTGATATTTTTATTGCTTTTATAGAGCGGTTCGCTTTCGCCGCGAATTTGGCGTTCAATAGATTCTCGTTGCGTGTGAAAACGGCGAAACGCGTCGTCTATCGTTTGTCGTTGCTTTTGCACTTGGTTTTCAACAGAACGACTGTCGCCCGCCAAGCCTTGCCGGCG  >  1/1475817‑1476284

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑