Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,475,991	C→T	V29V (GTG→GTA)	unknown ←	tRNA cytosine(34) acetyltransferase (EC 2.3.1.193)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,475,991	0	C	T	100.0%	148.9 / NA	53	V29V (GTG→GTA)	unknown	tRNA cytosine(34) acetyltransferase (EC 2.3.1.193)
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/35);  total (18/35)

TGTCGCCGTCGGGTTGTGCCGCCAAATCCTGCCACTCATTGAATAACAGCAACAATGTGCCGCCTTCCGACAACGCACCGGCGGCAATAGCGAGGGCATCTAAATGAATGCCTTGGCGCCCATCATAAATGAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAATGAAGTTGAGCGGAAAAGTGCGGCAAAACACCGCCAATCCACACCGCACTTTTCAGGGCAGGAAAGTATTCCCATTGGCGCGCTAACGTGGCATCGTCGCCAACAAGGAGAATTAGCTGCCGCGCCATGCTTTACCAACCCCGCGAATACGGATCCATCCAGTCTACATTGATATTTTTATTGCTTTTATAGAGCGGTTCGCTTTCGCCGCGAATTTGGCGTTCAATAGATTCTCGTTGCGTGTGAAAACGGCGAAACGCGTCGTCTATCGTTTGTCGTTGCTT  >  1/1475768‑1476233                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                   tgtcaccatcaggttgcaccgccAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGaaaatgt                                                                                                                                                                                                                         <  1:309866/233‑5 (MQ=255)     cccatcaggttgcaccgccAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTccc                                                                                                                                                                                                                     <  1:253168/237‑1 (MQ=255)      ccatcaggttgcaccgccAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCa                                                                                                                                                                                                                    >  1:63206/14‑251 (MQ=255)            ggttgcaccgccAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAACAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCAc                                                                                                                                                                                                                                             >  1:893088/8‑220 (MQ=255)             gttgcaccgccAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTgg                                                                                                                                                                                                                <  1:2743/242‑1 (MQ=255)             gttgcaccgccAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTgg                                                                                                                                                                                                                <  1:756796/242‑1 (MQ=255)                     gccAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGt                                                                                                                                                                                                     >  1:29227/1‑251 (MQ=255)                      ccAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGt                                                                                                                                                                                                     <  1:630816/250‑1 (MQ=255)                      ccAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGt                                                                                                                                                                                                     <  1:59991/250‑1 (MQ=255)                                   atCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACcaa                                                                                                                                                                                       <  1:307769/250‑1 (MQ=255)                                    tCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACcaat                                                                                                                                                                                      >  1:867890/1‑250 (MQ=255)                                    tCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACcaa                                                                                                                                                                                       >  1:14211/1‑250 (MQ=255)                                         gAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACcaatgagaa                                                                                                                                                                                 >  1:104009/1‑245 (MQ=255)                                           aTAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACcaatgagaaga                                                                                                                                                                               <  1:726572/251‑9 (MQ=255)                                                  caacaaTATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTaact                                                                                                                                                                        <  1:470535/251‑4 (MQ=255)                                                  caacaaTATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTaact                                                                                                                                                                        <  1:870287/251‑4 (MQ=255)                                                  caacaaTATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTaa                                                                                                                                                                          >  1:56150/1‑248 (MQ=255)                                                    acaaTATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCAtcgtcacc                                                                                                                                                                                         <  1:411686/232‑4 (MQ=255)                                                    acaaTATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTaac                                                                                                                                                                         >  1:271834/1‑246 (MQ=255)                                                    acaaTATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTAACTg                                                                                                                                                                       >  1:300262/1‑250 (MQ=255)                                                    acaaTATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTAACTg                                                                                                                                                                       >  1:193059/1‑250 (MQ=255)                                                               ccTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCTGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTAACTGCCGCGCCAtag                                                                                                                                                            <  1:212219/250‑3 (MQ=255)                                                                 ttCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTAACTGCCGCGCCAtagttt                                                                                                                                                         >  1:248402/1‑246 (MQ=255)                                                                      acagcGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAggg                                                                                                                                                                                                                                    <  1:71496/167‑1 (MQ=255)                                                                                     cAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTAACTGCCGCGCCATAGTTTACCAACCCCGTGAATACGGa                                                                                                                                     <  1:179552/251‑1 (MQ=255)                                                                                       atggCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTAACTGCCGCGCCATAGTTTACCAACCCCGTGAATACGGatc                                                                                                                                   <  1:238826/248‑1 (MQ=255)                                                                                         ggCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTAACTGCCGCGCCAtagt                                                                                                                                                           <  1:592869/224‑4 (MQ=255)                                                                                              gagCATCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTAACTGCCGCGCCATAGTTTACCAACCCCGTGAATACGGatccgtcc                                                                                                                              <  1:28446/247‑1 (MQ=255)                                                                                                 cATCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTAACTGCCGCGCCATAGTTTACCAACCCCGTGAATACGGatc                                                                                                                                   <  1:301001/241‑1 (MQ=255)                                                                                                  aTCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGc                                                                                                                                                                                                                                   <  1:27031/144‑1 (MQ=255)                                                                                                  aTCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTAACTGCCGCGCCAtagttt                                                                                                                                                         <  1:492585/218‑6 (MQ=255)                                                                                                                 ctgacgcCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTAACTGCCGCGCCATAGTTTACCAACCCCGTGAATACGGATCCGTCCAATCTACAtt                                                                                                                    <  1:558484/236‑1 (MQ=255)                                                                                                                 ctgacgcCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTAACTGCCGCGCCATAGTTTACCAACCCCGTGAATACGGATCCGTCCAATCTACATTAATATTTTTAt                                                                                                         <  1:160657/247‑1 (MQ=255)                                                                                                                           tcatAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACcaatg                                                                                                                                                                                     <  1:23565/165‑3 (MQ=255)                                                                                                                           tcatAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTAACTGCCGCGCCATAGTTTACCAACCCCGTGAATACGGatc                                                                                                                                   >  1:373470/1‑215 (MQ=255)                                                                                                                           tcatAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACCTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTAACTGCCGCGCCATAGTTTACCAACCCCGTGAATACGGa                                                                                                                                     <  1:437524/213‑1 (MQ=255)                                                                                                                            catAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTAACTGCCGCGCCATAGTTTACCAACCCCGTGAATACGGATCCGTCCAATCTACATTAATATTTTTATTGCTTTTATag                                                                                              <  1:823761/251‑1 (MQ=255)                                                                                                                            catAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTAACTGCCGCGCCATAGTTTACCAACCCCGTGAATACGGATCCGTCCAATCTACATTAATATTTTTATTGCTTTTATag                                                                                              <  1:590757/251‑1 (MQ=255)                                                                                                                            catAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTAACTGCCGCGCCATAGTTTACCAACCCCGTGAATACGGATCCGTCCAATCTACATTAATATTTTTATTGCTTTTATag                                                                                              <  1:11930/251‑1 (MQ=255)                                                                                                                             ataaataaGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTAACTGCCGCGCCATAGTTTACCAACCCCGTGAATACGGATCCGTCCAATCTACATTAATATTTTTATTGCTTTTATag                                                                                              <  1:154465/250‑1 (MQ=255)                                                                                                                              tAAATAAGCGCGAGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTAACTGCCGCGCCATAGTTTACCAACCCCGTGAATACGGATCCGTCCAATCTACATTAATATTTTTATTGCTTTTATagag                                                                                            <  1:467338/251‑1 (MQ=255)                                                                                                                                          gggAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTAACTGCCGCGCCATAGTTTACCAACCCCGTGAATACGGATCCGTCCAATCTACATTAata                                                                                                                <  1:577465/219‑1 (MQ=255)                                                                                                                                            gAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTAACTGCCGCGCCATAGTTTACCAACCCCGTGAATACGGa                                                                                                                                     <  1:647836/196‑1 (MQ=255)                                                                                                                                                tCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCAc                                                                                                                                                                                                                                             <  1:575030/88‑1 (MQ=255)                                                                                                                                                              aTTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTAACTGCCGCGCCATAGTTTACCAACCCCGTGAATACGGATCCGTCCAATCTACATTAATATTTTTATTGCTTTTATAGAGCGGTTCACTTTCGCCGCGAATTTGGCGTTCaa                                                            >  1:108532/1‑251 (MQ=255)                                                                                                                                                                         tGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTAACTGCCGCGCCATAGTTTACCAACCCCGTGAATACGGATCCGTCCAATCTACATTAATATTTTTATTGCTTTTATAGAGCGGTTCACTTTCGCCGCGAATTTGGCGTTCAATAGATTCTCGt                                                 <  1:198051/251‑1 (MQ=255)                                                                                                                                                                           cTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTAACTGCCGCGCCATAGTTTACCAACCCCGTGAATACGGATCCGTCCAATCTACATTAATATTTTTATTGCTTTTATAGAGCGGTTCACTTTCGCCGCGAATTTGGCGTTCAATAGATTCTCGTTg                                               <  1:572754/251‑1 (MQ=255)                                                                                                                                                                                          ggctGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTAACTGCCGCGCCATAGTTTACCAACCCCGTGAATACGGATCCGTCCAATCTACATTAATATTTTTATTGCTTTTATAGAGCGGTTCACTTTCGCCGCGAATTTGGCGTTCAATAGATTCTCGTTGCGTGTGAAAACGGCg                                >  1:367699/4‑251 (MQ=255)                                                                                                                                                                                                        gtgcggtgaaaCGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTAACTGCCGCGCCATAGTTTACCAACCCCGTGAATACGGATCCGTCCAATCTACATTAATATTTTTATTGCTTTTATAGAGCGGTTCACTTTCGCCGCGAATTTGGCGTTCAATAGATTCTCGTTGCGT‑TGAAAAccaaatgc                             >  1:123204/9‑232 (MQ=255)                                                                                                                                                                                                             gtgaaaCGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTAACTGCCGCGCCATAGTTTACCAACCCCGTGAATACGGATCCGTCCAATCTACATTAATATTTTTATTGCTTTTATAGAGCGGTTCACTTTCGCCGCGAATTTGGCGTTCAATAGATTCTCGTTg                                               >  1:5892/4‑217 (MQ=255)                                                                                                                                                                                                             gtgaaaCGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTAACTGCCGCGCCATAGTTTACCAACCCCGTGAATACGGATCCGTCCAATCTACATTAATATTTTTATTGCTTTTATAGAGCGGTTCACTTTCGCCGCGAATTTGGCGTTCAATAGATTCTCGTTGCGTGTGAAAACGGCGAAACGCATCGTCTATCGt              <  1:889965/247‑1 (MQ=255)                                                                                                                                                                                                                     ccGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTAACTGCCGCGCCATAGTTTACCAACCCCGTGAATACGAATCCGTCCAATCTACATTAATATTTTTATTGCTTTTATAGAGCGGTTCACTTTCGCCGCGAATTTGGCGTTCAATAGATTCTCGTTGCGTGTGAAAACGGCGAAACGCATCGTCTATCGTTTGTCGTTg     >  1:214952/1‑251 (MQ=255)                                                                                                                                                                                                                           aTCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTAACTGCCGCGCCATAGTTTACCAACCCCGTGAATACGGATCCGTCCAATCTACATTAATATTTTTATTGCTTTTATAGAGCGGTTCACTTTCGCCGCGAATTTGGCGTTCAATAGATTCTCGTTGCGTGTGAAAACGGCGAAACGCATCGTCTATCGTTTGTCGTTGCtt  >  1:210647/1‑248 (MQ=255)                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                   TGTCGCCGTCGGGTTGTGCCGCCAAATCCTGCCACTCATTGAATAACAGCAACAATGTGCCGCCTTCCGACAACGCACCGGCGGCAATAGCGAGGGCATCTAAATGAATGCCTTGGCGCCCATCATAAATGAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAATGAAGTTGAGCGGAAAAGTGCGGCAAAACACCGCCAATCCACACCGCACTTTTCAGGGCAGGAAAGTATTCCCATTGGCGCGCTAACGTGGCATCGTCGCCAACAAGGAGAATTAGCTGCCGCGCCATGCTTTACCAACCCCGCGAATACGGATCCATCCAGTCTACATTGATATTTTTATTGCTTTTATAGAGCGGTTCGCTTTCGCCGCGAATTTGGCGTTCAATAGATTCTCGTTGCGTGTGAAAACGGCGAAACGCGTCGTCTATCGTTTGTCGTTGCTT  >  1/1475768‑1476233

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑