Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,475,840	A→G	L80L (TTG→CTG)	unknown ←	tRNA cytosine(34) acetyltransferase (EC 2.3.1.193)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,475,840	0	A	G	100.0%	199.0 / NA	72	L80L (TTG→CTG)	unknown	tRNA cytosine(34) acetyltransferase (EC 2.3.1.193)
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/47);  total (25/47)

GTTCCAAGGTCGGTTGGTGTTGTTCTGAAAGCGACTTTTCAATCTTAGGGAAAACGAGCGCAGACGGCAAATGTTGATAAATGGGGAAGCTGTATTTCCGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACTGCTCCAACGCAGGCTGTCGCCGTCGGGTTGTGCCGCCAAATCCTGCCACTCATTGAATAACAGCAACAATGTGCCGCCTTCCGACAACGCACCGGCGGCAATAGCGAGGGCATCTAAATGAATGCCTTGGCGCCCATCATAAATGAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAATGAAGTTGAGCGGAAAAGTGCGGCAAAACACCGCCAATCCACACCGCACTTTTCAGGGCAGGAAAGTATTCCCATTGGCGCGCTAACGTGGCATCGTCGCCAACAAGGAGAATTAGCTGCCGCGCCATGCTTT  >  1/1475597‑1476082                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                   gTTCCAAGGTCGGTTGATGTAGTTCTGAAAGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACcg                                                                                                                                                                                                                                             >  1:840272/1‑251 (MQ=255)          tCGGTTGATGTAGTTCTGAAAGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAAtg                                                                                                                                                                                                                                    <  1:49567/251‑2 (MQ=255)          tCGGTTGATGTAGTTCTGAAAGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAAt                                                                                                                                                                                                                                     <  1:578240/250‑1 (MQ=255)           cGGTTGATGTAGTTCTGAAAGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTTTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAAtgg                                                                                                                                                                                                                                   <  1:739493/251‑3 (MQ=255)             gTTGATGTAGTTCTGAAAGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACcggcg                                                                                                                                                                                                                                          <  1:182502/242‑1 (MQ=255)             gTTGATGTAGTTCTGAAAGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCatattg                                                                                                                                                                                                                                  <  1:650412/250‑6 (MQ=255)                  tgtagttCTGAAAGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAgagc                                                                                                                                                                                                                            <  1:320599/247‑4 (MQ=255)                          tGAAAGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAaat                                                                                                                                                                                                                    <  1:7795/251‑1 (MQ=255)                            aaaGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAaatg                                                                                                                                                                                                                   <  1:132686/250‑1 (MQ=255)                            aaaGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAaatg                                                                                                                                                                                                                   <  1:726986/250‑1 (MQ=255)                            aaaGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAaatg                                                                                                                                                                                                                   <  1:373382/250‑1 (MQ=255)                                  acgtttCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGccc                                                                                                                                                                                                            <  1:722041/248‑2 (MQ=255)                                     tttCAATCCTAGGGAAGGCCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAaatg                                                                                                                                                                                                                   <  1:774487/241‑1 (MQ=255)                                        cAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGccctgacgc                                                                                                                                                                                                      <  1:7940/251‑8 (MQ=255)                                             ctAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCcat                                                                                                                                                                                                  >  1:703132/2‑250 (MQ=255)                                                     gaccagcgcgggcGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCGACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAaatg                                                                                                                                                                                                                   >  1:424225/13‑225 (MQ=255)                                                      accagcgcgggcGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAAta                                                                                                                                                                                          <  1:734236/238‑2 (MQ=255)                                                      accagcgcgggcGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAAta                                                                                                                                                                                          <  1:545766/238‑2 (MQ=255)                                                      accagcgcgggcGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAAta                                                                                                                                                                                          <  1:51859/238‑2 (MQ=255)                                                      accagcgcgggcGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAAta                                                                                                                                                                                          <  1:467746/238‑2 (MQ=255)                                                      accagcgcgggcGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAAta                                                                                                                                                                                          <  1:466534/238‑2 (MQ=255)                                                      acaagcgcgggcGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGACAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAAta                                                                                                                                                                                          <  1:582163/238‑2 (MQ=255)                                                           cgcgggcGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAATAAgcgc                                                                                                                                                                                     <  1:591353/243‑1 (MQ=255)                                                             cgggcGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCgggg                                                                                                                                                                                 <  1:802091/247‑1 (MQ=255)                                                                       atattGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCGGGGAATTCGc                                                                                                                                                                          <  1:473493/245‑1 (MQ=255)                                                                                 aTGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGAtt                                                                                                                                                             >  1:36715/1‑251 (MQ=255)                                                                                                                       ggCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAaatg                                                                                                                                                                                                                   >  1:116375/1‑159 (MQ=255)                                                                                                                       ggCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGaaa                                                                                                                       >  1:163458/1‑251 (MQ=255)                                                                                                                        gCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAATAAgcgc                                                                                                                                                                                     <  1:335624/188‑1 (MQ=255)                                                                                                                        gCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGaaaa                                                                                                                      >  1:25093/1‑251 (MQ=255)                                                                                                                        gCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGaaaa                                                                                                                      >  1:322993/1‑251 (MQ=255)                                                                                                                        gCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGaaa                                                                                                                       <  1:239994/250‑1 (MQ=255)                                                                                                                        gCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGaaa                                                                                                                       <  1:552108/250‑1 (MQ=255)                                                                                                                        gCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGaaa                                                                                                                       <  1:151475/250‑1 (MQ=255)                                                                                                                        gCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGATCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGaaa                                                                                                                       <  1:546230/250‑1 (MQ=255)                                                                                                                         cAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAg                                                                                                                     <  1:436916/251‑1 (MQ=255)                                                                                                                           aTAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAaatg                                                                                                                                                                                                                   >  1:782028/1‑155 (MQ=255)                                                                                                                           aTAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTg                                                                                                                   >  1:37136/1‑251 (MQ=255)                                                                                                                                     gtgtgtTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCggtgaaacg                                                                                                         >  1:409167/1‑244 (MQ=255)                                                                                                                                         gtTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCggtgaaacgccg                                                                                                      >  1:719897/1‑240 (MQ=255)                                                                                                                                         gtTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCggtgaaacgc                                                                                                        <  1:71257/248‑9 (MQ=255)                                                                                                                                             aTCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAAt                                                                                                 >  1:642971/1‑251 (MQ=255)                                                                                                                                              tCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATc                                                                                                <  1:210987/251‑1 (MQ=255)                                                                                                                                               cGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATcc                                                                                               <  1:212676/251‑1 (MQ=255)                                                                                                                                                   tgtTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCcatac                                                                                           <  1:235089/251‑4 (MQ=255)                                                                                                                                                              gCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCggtatggattggcggcgtttcac                                                                                           <  1:335772/240‑22 (MQ=255)                                                                                                                                                                            tgtcaccatcaggttgcaccgccAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGaaaatgt                                                                  <  1:309866/233‑5 (MQ=255)                                                                                                                                                                                cccatcaggttgcaccgccAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTccc                                                              <  1:253168/237‑1 (MQ=255)                                                                                                                                                                                 ccatcaggttgcaccgccAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGAtttt                                                                                                                                                           >  1:485204/14‑157 (MQ=255)                                                                                                                                                                                 ccatcaggttgcaccgccAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCa                                                             >  1:63206/14‑251 (MQ=255)                                                                                                                                                                                       ggttgcaccgccAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAACAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCAc                                                                                      >  1:893088/8‑220 (MQ=255)                                                                                                                                                                                        gttgcaccgccAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTgg                                                         <  1:2743/242‑1 (MQ=255)                                                                                                                                                                                        gttgcaccgccAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTgg                                                         <  1:756796/242‑1 (MQ=255)                                                                                                                                                                                                gccAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGt                                              >  1:29227/1‑251 (MQ=255)                                                                                                                                                                                                 ccAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGt                                              <  1:59991/250‑1 (MQ=255)                                                                                                                                                                                                 ccAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGt                                              <  1:630816/250‑1 (MQ=255)                                                                                                                                                                                                              atCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACcaa                                <  1:307769/250‑1 (MQ=255)                                                                                                                                                                                                               tCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACcaat                               >  1:867890/1‑250 (MQ=255)                                                                                                                                                                                                               tCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACcaa                                >  1:14211/1‑250 (MQ=255)                                                                                                                                                                                                                  ttGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCggtga                                                                                                             <  1:382846/170‑4 (MQ=255)                                                                                                                                                                                                                    gAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACcaatgagaa                          >  1:104009/1‑245 (MQ=255)                                                                                                                                                                                                                      aTAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACcaatgagaaga                        <  1:726572/251‑9 (MQ=255)                                                                                                                                                                                                                             caacaaTATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTaact                 <  1:470535/251‑4 (MQ=255)                                                                                                                                                                                                                             caacaaTATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTaact                 <  1:870287/251‑4 (MQ=255)                                                                                                                                                                                                                             caacaaTATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTaa                   >  1:56150/1‑248 (MQ=255)                                                                                                                                                                                                                               acaaTATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCAtcgtcacc                                  <  1:411686/232‑4 (MQ=255)                                                                                                                                                                                                                               acaaTATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTaac                  >  1:271834/1‑246 (MQ=255)                                                                                                                                                                                                                               acaaTATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTAACTg                >  1:300262/1‑250 (MQ=255)                                                                                                                                                                                                                               acaaTATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTAACTg                >  1:193059/1‑250 (MQ=255)                                                                                                                                                                                                                                          ccTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCTGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTAACTGCCGCGCCAtag     <  1:212219/250‑3 (MQ=255)                                                                                                                                                                                                                                            ttCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGTGGCATCGTCACCAATGAGAAGAATTAACTGCCGCGCCAtagttt  >  1:248402/1‑246 (MQ=255)                                                                                                                                                                                                                                             tCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCcat                                                                                             <  1:287215/159‑2 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                   GTTCCAAGGTCGGTTGGTGTTGTTCTGAAAGCGACTTTTCAATCTTAGGGAAAACGAGCGCAGACGGCAAATGTTGATAAATGGGGAAGCTGTATTTCCGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACTGCTCCAACGCAGGCTGTCGCCGTCGGGTTGTGCCGCCAAATCCTGCCACTCATTGAATAACAGCAACAATGTGCCGCCTTCCGACAACGCACCGGCGGCAATAGCGAGGGCATCTAAATGAATGCCTTGGCGCCCATCATAAATGAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAATGAAGTTGAGCGGAAAAGTGCGGCAAAACACCGCCAATCCACACCGCACTTTTCAGGGCAGGAAAGTATTCCCATTGGCGCGCTAACGTGGCATCGTCGCCAACAAGGAGAATTAGCTGCCGCGCCATGCTTT  >  1/1475597‑1476082

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑