Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,475,802	C→A	E92D (GAG→GAT)	unknown ←	tRNA cytosine(34) acetyltransferase (EC 2.3.1.193)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,475,802	0	C	A	100.0%	198.1 / NA	72	E92D (GAG→GAT)	unknown	tRNA cytosine(34) acetyltransferase (EC 2.3.1.193)
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (25/47);  total (25/47)

CAGATGTCGCCATTTGCCGCAATAAATGGGCTTGTTCCAAGGTCGGTTGGTGTTGTTCTGAAAGCGACTTTTCAATCTTAGGGAAAACGAGCGCAGACGGCAAATGTTGATAAATGGGGAAGCTGTATTTCCGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACTGCTCCAACGCAGGCTGTCGCCGTCGGGTTGTGCCGCCAAATCCTGCCACTCATTGAATAACAGCAACAATGTGCCGCCTTCCGACAACGCACCGGCGGCAATAGCGAGGGCATCTAAATGAATGCCTTGGCGCCCATCATAAATGAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAATGAAGTTGAGCGGAAAAGTGCGGCAAAACACCGCCAATCCACACCGCACTTTTCAGGGCAGGAAAGTATTCCCATTGGCGCGCTAACGT  >  1/1475564‑1476038                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                             cggATGTCGCCATTTGCCGCAATAAATGGGTTTGTTCCAAGGTCGGTTGATGTAGTTCTGAAAGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAAc                                                                                                                                                                                                                                  <  1:775271/249‑1 (MQ=255)    aTGTCGCCATTTGCCGCAATAAATGGGTTTGTTCCAAGGTCGGTTGATGTAGTTCTGAAAGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAg                                                                                                                                                                                                                                <  1:661606/250‑1 (MQ=255)               tGCCGCAATAAATGGGTTTGTTCCAAGGTCGGTTGATGTAGTTCTGAAAGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATgcc                                                                                                                                                                                                                    <  1:195416/251‑1 (MQ=255)                    cAATAAATGGGTTTGTTCCAAGGTCGGTTGATGTAGTTCTGAAAGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCtt                                                                                                                                                                                                               >  1:581539/1‑251 (MQ=255)                      aTAAATGGGTTTGTTCCAAGGTCGGTTGATGTAGTTCTGAAAGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTc                                                                                                                                                                                                              >  1:168614/1‑250 (MQ=255)                        aaaTGGGTTTGTTCCAAGGTCGGTTGATGTAGTTCTGAAAGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGcaacaa                                                                                                                                                                                                                          >  1:181389/1‑236 (MQ=255)                        aaaTGGGTTTGTTCCAAGGTCGGTTGATGTAGTTCTGAAAGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATgc                                                                                                                                                                                                                     >  1:755147/1‑241 (MQ=255)                        aaaTGGGTTTGTTCCAAGGTCGGTTGATGTAGTTCTGAAAGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGa                                                                                                                                                                                                           >  1:58554/1‑251 (MQ=255)                         aaTGGGTTTGTTCCAAGGTCGGTTGATGTAGTTCTGAAAGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGAc                                                                                                                                                                                                          <  1:408960/251‑1 (MQ=255)                         aaTGGGTTTGTTCCAAGGTCGGTTGATGTAGTTCTGAAAGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGAc                                                                                                                                                                                                          <  1:380027/251‑1 (MQ=255)                             ggtttGTTCCAAGGTCGGTTGATGTAGTTCTGAAAGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACagcg                                                                                                                                                                                                      >  1:200952/4‑248 (MQ=255)                                  gTTCCAAGGTCGGTTGATGTAGTTCTGAAAGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAAc                                                                                                                                                                                                                                  <  1:49114/218‑1 (MQ=255)                                  gTTCCAAGGTCGGTTGATGTAGTTCTGAAAGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACcg                                                                                                                                                                                                 >  1:840272/1‑251 (MQ=255)                                           tCGGTTGATGTAGTTCTGAAAGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAAtg                                                                                                                                                                                        <  1:49567/251‑2 (MQ=255)                                           tCGGTTGATGTAGTTCTGAAAGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAAt                                                                                                                                                                                         <  1:578240/250‑1 (MQ=255)                                            cGGTTGATGTAGTTCTGAAAGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTTTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAAtgg                                                                                                                                                                                       <  1:739493/251‑3 (MQ=255)                                              gTTGATGTAGTTCTGAAAGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGAc                                                                                                                                                                                                          <  1:120324/230‑1 (MQ=255)                                              gTTGATGTAGTTCTGAAAGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACcggcg                                                                                                                                                                                              <  1:182502/242‑1 (MQ=255)                                              gTTGATGTAGTTCTGAAAGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCatattg                                                                                                                                                                                      <  1:650412/250‑6 (MQ=255)                                                   tgtagttCTGAAAGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAgagc                                                                                                                                                                                <  1:320599/247‑4 (MQ=255)                                                           tGAAAGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAaat                                                                                                                                                                        <  1:7795/251‑1 (MQ=255)                                                             aaaGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAaatg                                                                                                                                                                       <  1:132686/250‑1 (MQ=255)                                                             aaaGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAaatg                                                                                                                                                                       <  1:726986/250‑1 (MQ=255)                                                             aaaGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAaatg                                                                                                                                                                       <  1:373382/250‑1 (MQ=255)                                                                   acgtttCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGccc                                                                                                                                                                <  1:722041/248‑2 (MQ=255)                                                                      tttCAATCCTAGGGAAGGCCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAaatg                                                                                                                                                                       <  1:774487/241‑1 (MQ=255)                                                                      tttCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAAc                                                                                                                                                                                                                                  <  1:263503/182‑1 (MQ=255)                                                                         cAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGccctgacgc                                                                                                                                                          <  1:7940/251‑8 (MQ=255)                                                                              ctAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCcat                                                                                                                                                      >  1:703132/2‑250 (MQ=255)                                                                                      gaccagcgcgggcGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCGACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAaatg                                                                                                                                                                       >  1:424225/13‑225 (MQ=255)                                                                                       accagcgcgggcGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAAta                                                                                                                                              <  1:734236/238‑2 (MQ=255)                                                                                       accagcgcgggcGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAAta                                                                                                                                              <  1:466534/238‑2 (MQ=255)                                                                                       accagcgcgggcGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAAta                                                                                                                                              <  1:467746/238‑2 (MQ=255)                                                                                       accagcgcgggcGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAAta                                                                                                                                              <  1:545766/238‑2 (MQ=255)                                                                                       accagcgcgggcGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAAta                                                                                                                                              <  1:51859/238‑2 (MQ=255)                                                                                       acaagcgcgggcGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGACAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAAta                                                                                                                                              <  1:582163/238‑2 (MQ=255)                                                                                            cgcgggcGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAATAAgcgc                                                                                                                                         <  1:591353/243‑1 (MQ=255)                                                                                              cgggcGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCgggg                                                                                                                                     <  1:802091/247‑1 (MQ=255)                                                                                                        atattGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCGGGGAATTCGc                                                                                                                              <  1:473493/245‑1 (MQ=255)                                                                                                              aTAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATgcc                                                                                                                                                                                                                    >  1:245815/1‑156 (MQ=255)                                                                                                                  aTGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGAtt                                                                                                                 >  1:36715/1‑251 (MQ=255)                                                                                                                                                        ggCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAaatg                                                                                                                                                                       >  1:116375/1‑159 (MQ=255)                                                                                                                                                        ggCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGaaa                                                                           >  1:163458/1‑251 (MQ=255)                                                                                                                                                         gCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGAc                                                                                                                                                                                                          >  1:83046/1‑123 (MQ=255)                                                                                                                                                         gCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAATAAgcgc                                                                                                                                         <  1:335624/188‑1 (MQ=255)                                                                                                                                                         gCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGaaaa                                                                          >  1:322993/1‑251 (MQ=255)                                                                                                                                                         gCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGaaaa                                                                          >  1:25093/1‑251 (MQ=255)                                                                                                                                                         gCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGaaa                                                                           <  1:552108/250‑1 (MQ=255)                                                                                                                                                         gCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGaaa                                                                           <  1:151475/250‑1 (MQ=255)                                                                                                                                                         gCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGaaa                                                                           <  1:239994/250‑1 (MQ=255)                                                                                                                                                         gCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGATCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGaaa                                                                           <  1:546230/250‑1 (MQ=255)                                                                                                                                                          cAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAg                                                                         <  1:436916/251‑1 (MQ=255)                                                                                                                                                            aTAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAaatg                                                                                                                                                                       >  1:782028/1‑155 (MQ=255)                                                                                                                                                            aTAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTg                                                                       >  1:37136/1‑251 (MQ=255)                                                                                                                                                                      gtgtgtTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCggtgaaacg                                                             >  1:409167/1‑244 (MQ=255)                                                                                                                                                                          gtTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCggtgaaacgccg                                                          >  1:719897/1‑240 (MQ=255)                                                                                                                                                                          gtTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCggtgaaacgc                                                            <  1:71257/248‑9 (MQ=255)                                                                                                                                                                              aTCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAAt                                                     >  1:642971/1‑251 (MQ=255)                                                                                                                                                                               tCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATc                                                    <  1:210987/251‑1 (MQ=255)                                                                                                                                                                                cGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATcc                                                   <  1:212676/251‑1 (MQ=255)                                                                                                                                                                                    tgtTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCcatac                                               <  1:235089/251‑4 (MQ=255)                                                                                                                                                                                               gCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCggtatggattggcggcgtttcac                                               <  1:335772/240‑22 (MQ=255)                                                                                                                                                                                                             tgtcaccatcaggttgcaccgccAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGaaaatgt                      <  1:309866/233‑5 (MQ=255)                                                                                                                                                                                                                 cccatcaggttgcaccgccAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTccc                  <  1:253168/237‑1 (MQ=255)                                                                                                                                                                                                                  ccatcaggttgcaccgccAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGAtttt                                                                                                               >  1:485204/14‑157 (MQ=255)                                                                                                                                                                                                                  ccatcaggttgcaccgccAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCa                 >  1:63206/14‑251 (MQ=255)                                                                                                                                                                                                                        ggttgcaccgccAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAACAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCAc                                          >  1:893088/8‑220 (MQ=255)                                                                                                                                                                                                                         gttgcaccgccAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTgg             <  1:756796/242‑1 (MQ=255)                                                                                                                                                                                                                         gttgcaccgccAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTgg             <  1:2743/242‑1 (MQ=255)                                                                                                                                                                                                                                 gccAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGt  >  1:29227/1‑251 (MQ=255)                                                                                                                                                                                                                                  ccAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGt  <  1:630816/250‑1 (MQ=255)                                                                                                                                                                                                                                  ccAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAGTGAGGCTGAGCAGAAAAGTGCGGTGAAACGCCGCCAATCCATACCGCACTTTTCAGGGCAGGAAAATGTTCCCATTGGTGCGCTAACGt  <  1:59991/250‑1 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                             CAGATGTCGCCATTTGCCGCAATAAATGGGCTTGTTCCAAGGTCGGTTGGTGTTGTTCTGAAAGCGACTTTTCAATCTTAGGGAAAACGAGCGCAGACGGCAAATGTTGATAAATGGGGAAGCTGTATTTCCGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACTGCTCCAACGCAGGCTGTCGCCGTCGGGTTGTGCCGCCAAATCCTGCCACTCATTGAATAACAGCAACAATGTGCCGCCTTCCGACAACGCACCGGCGGCAATAGCGAGGGCATCTAAATGAATGCCTTGGCGCCCATCATAAATGAGCGCGGGGAATTCGCTGCCGAGCAGATTTTTACTTTTGCTAAAAGGGAAATGAAGTTGAGCGGAAAAGTGCGGCAAAACACCGCCAATCCACACCGCACTTTTCAGGGCAGGAAAGTATTCCCATTGGCGCGCTAACGT  >  1/1475564‑1476038

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑