Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,475,687	T→C	S131G (AGC→GGC)	unknown ←	tRNA cytosine(34) acetyltransferase (EC 2.3.1.193)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,475,687	0	T	C	100.0%	217.2 / NA	76	S131G (AGC→GGC)	unknown	tRNA cytosine(34) acetyltransferase (EC 2.3.1.193)
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (30/46);  total (30/46)

TCAATAGAACGGGCTTATTTGCCGCTAATTGCTGTTTGGCGAACAAGCCCGCCAAAGCGGATTTTCCCCGCCCGCGCTTTGCCGTAACGATTAACATCTCCGCGTCAGATGTCGCCATTTGCCGCAATAAATGGGCTTGTTCCAAGGTCGGTTGGTGTTGTTCTGAAAGCGACTTTTCAATCTTAGGGAAAACGAGCGCAGACGGCAAATGTTGATAAATGGGGAAGCTGTATTTCCGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACTGCTCCAACGCAGGCTGTCGCCGTCGGGTTGTGCCGCCAAATCCTGCCACTCATTGAATAACAGCAACAATGTGCCGCCTTCCGACAACGCACCGGCGGCAATAGCGAGGGCATCTAAATGAATGCCTTGGCGCCCATCATAAATGAGCGCGGGGAATTCGCTGCCGAGCAGATT  >  1/1475459‑1475927                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                 tCAATAGAACAGGCTGATTTGCTTCCAATTGCTGCTTGGCGAACAAGCCTGCCAAAGCGGATTTGCCCCGCCCACGCTTTGCCGTAACGATTAACATCTCTGCACCGGATGTCGCCATTTGCCGCAATAAATGGGTTTGTTCCAAGGTCGGTTGATGTAGTTCTGAAAGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGc                                                                                                                                                                                                                            <  1:324372/251‑1 (MQ=255)  cAATAGAACAGGCTGATTTGCTTCCAATTGCTGCTTGGCGAACAAGCCTGCCAAAGCGGATTTGCCCCGCCCACGCTTTGCCGTAACGATTAACATCTCTGCACCGGATGTCGCCATTTGCCGCAATAAATGGGTTTGTTCCAAGGTCGGTTGATGTAGTTCTGAAAGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCa                                                                                                                                                                                                                           >  1:660618/1‑251 (MQ=255)  cAATAGAACAGGCTGATTTGCTTCCAATTGCTGCTTGGCGAACAAGCCTGCCAAAGCGGATTTGCCCCGCCCACGCTTTGCCGTAACGATTAACATCTCTGCACCGGATGTCGCCATTTGCCGCAATAAATGGGTTTGTTCCAAGGTCGGTTGATGTAGTTCTGAAAGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCa                                                                                                                                                                                                                           >  1:65902/1‑251 (MQ=255)  cAATAGAACAGGCTGATTTGCTTCCAATTGCTGCTTGGCGAACAAGCCTGCCAAAGCGGATTTGCCCCGCCCACGCTTTGCCGTAACGATTAACATCTCTGCACCGGATGTCGCCATTTGCCGCAATAAATGGGTTTGTTCCAAGGTCGGTTGATGTAGTTCTGAAAGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCa                                                                                                                                                                                                                           >  1:578806/1‑251 (MQ=255)  cAATAGAACAGGCTGATTTGCTTCCAATTGCTGCTTGGCGAACAAGCCTGCCAAAGCGGATTTGCCCCGCCCACGCTTTGCCGTAACGATTAACATCTCTGCACCGGATGTCGCCATTTGCCGCAATAAATGGGTTTGTTCCAAGGTCGGTTGATGTAGTTCTGAAAGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCa                                                                                                                                                                                                                           >  1:471226/1‑251 (MQ=255)  cAATAGAACAGGCTGATTTGCTTCCAATTGCTGCTTGGCGAACAAGCCTGCCAAAGCGGATTTGCCCCGCCCACGCTTTGCCGTAACGATTAACATCTCTGCACCGGATGTCGCCATTTGCCGCAATAAATGGGTTTGTTCCAAGGTCGGTTGATGTAGTTCTGAAAGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCa                                                                                                                                                                                                                           >  1:396196/1‑251 (MQ=255)       gaacaggctgatttgcttccaaTTGCTGCTTGGCGAACAAGCCTGCCAAAGCGGATTTGCCCCGCCCACGCTTTGCCGTAACGATTAACATCTCTGCACCGGATGTCGCCATTTGCCGCAATAAATGGGTTTGTTCCAAGGTCGGTTGATGTAGTTCTGAAAGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAg                                                                                                                                                                                                                      >  1:515335/21‑251 (MQ=255)       gaacaggctgatttgcttccaaTTGCTGCTTGGCGAACAAGCCTGCCAAAGCGGATTTGCCCCGCCCACGCTTTGCCGTAACGATTAACATCTCTGCACCGGATGTCGCCATTTGCCGCAATAAATGGGTTTGTTCCAAGGTCGGTTGATGTAGTTCTGAAAGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAg                                                                                                                                                                                                                      >  1:204357/21‑251 (MQ=255)                            aTTGCTGCTTGGCGAACAAGCCTGCCAAAGCGGATTTGCCCCGCCCACGCTTTGCCGTAACGATTAACATCTCTGCACCGGATGTCGCCATTTGCCGCAATAAATGGGTTTGTTCCAAGGTCGGTTGATGTAGTTCTGAAAGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGttt                                                                                                                                                                                                 <  1:738563/251‑1 (MQ=255)                               gctgctTGGCGAACAAGCCTGCCAAAGCGGATTTGCCCCGCCCACGCTTTGCCGTAACGATTAACATCTCTGCACCGGATGTCGCCATTTGCCGCAATAAATGGGTTTGTTCCAAGGTCGGTTGATGTAGTTCTGAAAGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTAt                                                                                                                                                                                               >  1:669075/1‑250 (MQ=255)                                ctgcttGGCGAACAAGCCTGCCAAAGCGGATTTGCCCCGCCCACGCTTTGCCGTAACGATTAACATCTCTGCACCGGATGTCGCCATTTGCCGCAATAAATGGGTTTGTTCCAAGGTCGGTTGATGTAGTTCTAAAAGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATc                                                                                                                                                                                              <  1:463983/246‑1 (MQ=255)                                   cttGGCGAACAAGCCTGCCAAAGCGGATTTGCCCCGCCCACGCTTTGCCGTAACGATTAACATCTCTGCACCGGATGTCGCCATTTGCCGCAATAAATGGGTTTGTTCCAAGGTCGGTTGATGTAGTTCTGAAAGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCggg                                                                                                                                                                                           <  1:518565/249‑1 (MQ=255)                                         gAACAAGCCTGCCAAAGCGGATTTGCCCCGCCCACGCTTTGCCGTAACGATTAACATCTCTGCACCGGATGTCGCCATTTGCCGCAATAAATGGGTTTGTTCCAAGGTCGGTTGATGTAGTTCTGAAAGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGttttt                                                                                                                                                                                    >  1:104312/1‑251 (MQ=255)                                              aGCCTGCCAAAGCGGATTTGCCCCGCCCACGCTTTGCCGTAACGATTAACATCTCTGCACCGGATGTCGCCATTTGCCGCAATAAATGGGTTTGTTCCAAGGTCGGTTGATGTAGTTCTGAAAGCGACGTTTCACTCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCAccg                                                                                                                                                                               >  1:718452/1‑249 (MQ=255)                                                   gCCAAAGCGGATTTGCCCCGCCCACGCTTTGCCGTAACGATTAACATCTCTGCACCGGATGTCGCCATTTGCCGCAATAAATGGGTTTGTTCCAAGGTCGGTTGATGTAGTTCTGAAAGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGAGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCa                                                                                                                                                                          >  1:582660/1‑251 (MQ=255)                                                       aaGCGGATTTGCCCCGCCCACGCTTTGCCGTAACGATTAACATCTCTGCACCGGATGTCGCCATTTGCCGCAATAAATGGGTTTGTTCCAAGGTCGGTTGATGTAGTTCTGAAAGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACg                                                                                                                                                                       >  1:478547/1‑250 (MQ=255)                                                            gATTTGCCCCGCCCACGCTTTGCCGTAACGATTAACATCTCTGCACCGGATGTCGCCATTTGCCGCAATAAATGGGTTTGTTCCAAGGTCGGTTGATGTAGTTCTGAAAGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCaagc                                                                                                                                                                  <  1:639360/250‑4 (MQ=255)                                                            gATTTGCCCCGCCCACGCTTTGCCGTAACGATTAACATCTCTGCACCGGATGTCGCCATTTGCCGCAATAAATGGGTTTGTTCCAAGGTCGGTTGATGTAGTTCTGAAAGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCaag                                                                                                                                                                   >  1:241184/1‑247 (MQ=255)                                                            gATTTGCCCCGCCCACGCTTTGCCGTAACGATTAACATCTCTGCACCGGATGTCGCCATTTGCCGCAATAAATGGGTTTGTTCCAAGGTCGGTTGATGTAGTTCTGAAAGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCaag                                                                                                                                                                   >  1:296060/1‑247 (MQ=255)                                                                                       aCGATTAACATCTCTGCACCGGATGTCGCCATTTGCCGCAATAAATGGGTTTGTTCCAAGGTCGGTTGATGTAGTTCTGAAAGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATc                                                                                                                                      <  1:780503/251‑1 (MQ=255)                                                                                         gATTAACATCTCTGCACCGGATGTCGCCATTTGCCGCAATAAATGGGTTTGTTCCAAGGTCGGTTGATGTAGTTCTGAAAGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATc                                                                                                                                      >  1:707442/1‑249 (MQ=255)                                                                                          aTTAACATCTCTGCACCGGATGTCGCCATTTGCCGCAATAAATGGGTTTGTTCCAAGGTCGGTTGATGTAGTTCTGAAAGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGc                                                                                                                                                                      >  1:171207/1‑216 (MQ=255)                                                                                          aTTAACATCTCTGCACCGGATGTCGCCATTTGCCGCAATAAATGGGTTTGTTCCAAGGTCGGTTGATGTAGTTCTGAAAGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTcaccatcaggt                                                                                                                                                    <  1:539034/234‑11 (MQ=255)                                                                                                 tctctgcaccggATGTCGCCATTTGCCGCAATAAATGGGTTTGTTCCAAGGTCGGTTGATGTAGTTCTGAAAGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGc                                                                                                                                                                      >  1:253815/12‑209 (MQ=255)                                                                                                          cggATGTCGCCATTTGCCGCAATAAATGGGTTTGTTCCAAGGTCGGTTGATGTAGTTCTGAAAGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAAc                                                                                                                   <  1:775271/249‑1 (MQ=255)                                                                                                             aTGTCGCCATTTGCCGCAATAAATGGGTTTGTTCCAAGGTCGGTTGATGTAGTTCTGAAAGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAg                                                                                                                 <  1:661606/250‑1 (MQ=255)                                                                                                                        tGCCGCAATAAATGGGTTTGTTCCAAGGTCGGTTGATGTAGTTCTGAAAGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATgcc                                                                                                     <  1:195416/251‑1 (MQ=255)                                                                                                                             cAATAAATGGGTTTGTTCCAAGGTCGGTTGATGTAGTTCTGAAAGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATc                                                                                                                                                                                              >  1:26160/1‑157 (MQ=255)                                                                                                                             cAATAAATGGGTTTGTTCCAAGGTCGGTTGATGTAGTTCTGAAAGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTc                                                                                                                                                                                   <  1:101027/168‑1 (MQ=255)                                                                                                                             cAATAAATGGGTTTGTTCCAAGGTCGGTTGATGTAGTTCTGAAAGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTcacc                                                                                                                                                           <  1:187747/192‑4 (MQ=255)                                                                                                                             cAATAAATGGGTTTGTTCCAAGGTCGGTTGATGTAGTTCTGAAAGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCtt                                                                                                >  1:581539/1‑251 (MQ=255)                                                                                                                               aTAAATGGGTTTGTTCCAAGGTCGGTTGATGTAGTTCTGAAAGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTc                                                                                               >  1:168614/1‑250 (MQ=255)                                                                                                                                 aaaTGGGTTTGTTCCAAGGTCGGTTGATGTAGTTCTGAAAGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGcaacaa                                                                                                           >  1:181389/1‑236 (MQ=255)                                                                                                                                 aaaTGGGTTTGTTCCAAGGTCGGTTGATGTAGTTCTGAAAGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATgc                                                                                                      >  1:755147/1‑241 (MQ=255)                                                                                                                                 aaaTGGGTTTGTTCCAAGGTCGGTTGATGTAGTTCTGAAAGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGa                                                                                            >  1:58554/1‑251 (MQ=255)                                                                                                                                  aaTGGGTTTGTTCCAAGGTCGGTTGATGTAGTTCTGAAAGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGAc                                                                                           <  1:408960/251‑1 (MQ=255)                                                                                                                                  aaTGGGTTTGTTCCAAGGTCGGTTGATGTAGTTCTGAAAGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGAc                                                                                           <  1:380027/251‑1 (MQ=255)                                                                                                                                      ggtttGTTCCAAGGTCGGTTGATGTAGTTCTGAAAGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTAt                                                                                                                                                                                               <  1:520638/144‑1 (MQ=255)                                                                                                                                      ggtttGTTCCAAGGTCGGTTGATGTAGTTCTGAAAGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACagcg                                                                                       >  1:200952/4‑248 (MQ=255)                                                                                                                                           gTTCCAAGGTCGGTTGATGTAGTTCTGAAAGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGc                                                                                                                                                                                                          <  1:693442/131‑1 (MQ=255)                                                                                                                                           gTTCCAAGGTCGGTTGATGTAGTTCTGAAAGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCAc                                                                                                                                                                                 <  1:830534/156‑1 (MQ=255)                                                                                                                                           gTTCCAAGGTCGGTTGATGTAGTTCTGAAAGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAAc                                                                                                                   <  1:49114/218‑1 (MQ=255)                                                                                                                                           gTTCCAAGGTCGGTTGATGTAGTTCTGAAAGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACcg                                                                                  >  1:840272/1‑251 (MQ=255)                                                                                                                                                    tCGGTTGATGTAGTTCTGAAAGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAAtg                                                                         <  1:49567/251‑2 (MQ=255)                                                                                                                                                    tCGGTTGATGTAGTTCTGAAAGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAAt                                                                          <  1:578240/250‑1 (MQ=255)                                                                                                                                                     cGGTTGATGTAGTTCTGAAAGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTTTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAAtgg                                                                        <  1:739493/251‑3 (MQ=255)                                                                                                                                                       gTTGATGTAGTTCTGAAAGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGAc                                                                                           <  1:120324/230‑1 (MQ=255)                                                                                                                                                       gTTGATGTAGTTCTGAAAGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACcggcg                                                                               <  1:182502/242‑1 (MQ=255)                                                                                                                                                       gTTGATGTAGTTCTGAAAGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCatattg                                                                       <  1:650412/250‑6 (MQ=255)                                                                                                                                                           atgtagttCTGAAAGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGgtgt                                                                                                                                                                                                     <  1:597872/115‑1 (MQ=255)                                                                                                                                                            tgtagttCTGAAAGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAgagc                                                                 <  1:320599/247‑4 (MQ=255)                                                                                                                                                             gtagttCTGAAAGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCAc                                                                                                                                                                                 <  1:224027/135‑1 (MQ=255)                                                                                                                                                                    tGAAAGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAaat                                                         <  1:7795/251‑1 (MQ=255)                                                                                                                                                                      aaaGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAaatg                                                        <  1:132686/250‑1 (MQ=255)                                                                                                                                                                      aaaGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAaatg                                                        <  1:726986/250‑1 (MQ=255)                                                                                                                                                                      aaaGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAaatg                                                        <  1:373382/250‑1 (MQ=255)                                                                                                                                                                            acgtttCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGccc                                                 <  1:722041/248‑2 (MQ=255)                                                                                                                                                                               tttCAATCCTAGGGAAGGCCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAaatg                                                        <  1:774487/241‑1 (MQ=255)                                                                                                                                                                               tttCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGGTAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTcaccatc                                                                                                                                                        <  1:379950/145‑7 (MQ=255)                                                                                                                                                                               tttCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAAc                                                                                                                   <  1:263503/182‑1 (MQ=255)                                                                                                                                                                                  cAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGccctgacgc                                           <  1:7940/251‑8 (MQ=255)                                                                                                                                                                                       ctAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCcat                                       >  1:703132/2‑250 (MQ=255)                                                                                                                                                                                               gaccagcgcgggcGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCGACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAaatg                                                        >  1:424225/13‑225 (MQ=255)                                                                                                                                                                                                accagcgcgggcGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAAta                               <  1:545766/238‑2 (MQ=255)                                                                                                                                                                                                accagcgcgggcGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAAta                               <  1:734236/238‑2 (MQ=255)                                                                                                                                                                                                accagcgcgggcGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAAta                               <  1:466534/238‑2 (MQ=255)                                                                                                                                                                                                accagcgcgggcGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAAta                               <  1:467746/238‑2 (MQ=255)                                                                                                                                                                                                accagcgcgggcGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAAta                               <  1:51859/238‑2 (MQ=255)                                                                                                                                                                                                acaagcgcgggcGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGACAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAAta                               <  1:582163/238‑2 (MQ=255)                                                                                                                                                                                                     cgcgggcGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAATAAgcgc                          <  1:591353/243‑1 (MQ=255)                                                                                                                                                                                                       cgggcGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCgggg                      <  1:802091/247‑1 (MQ=255)                                                                                                                                                                                                           cGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATc                                                                                                                                                                                              <  1:464978/79‑1 (MQ=255)                                                                                                                                                                                                                 atattGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCGGGGAATTCGc               <  1:473493/245‑1 (MQ=255)                                                                                                                                                                                                                   attGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATaa                                                                                                                                                                                                               >  1:749422/2‑54 (MQ=255)                                                                                                                                                                                                                       aTAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATgcc                                                                                                     >  1:245815/1‑156 (MQ=255)                                                                                                                                                                                                                           aTGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATCCTGCCAATCATTGAATAACAGCAACAATATGCCGCCTTCCGACAGCGCACCGGCGGCAATGGCGAGAGCATCTAAATGAATGCCCTGACGCCCATCATAAATAAGCGCGGGGAATTCGCTGCCGAGCAGAtt  >  1:36715/1‑251 (MQ=255)                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                 TCAATAGAACGGGCTTATTTGCCGCTAATTGCTGTTTGGCGAACAAGCCCGCCAAAGCGGATTTTCCCCGCCCGCGCTTTGCCGTAACGATTAACATCTCCGCGTCAGATGTCGCCATTTGCCGCAATAAATGGGCTTGTTCCAAGGTCGGTTGGTGTTGTTCTGAAAGCGACTTTTCAATCTTAGGGAAAACGAGCGCAGACGGCAAATGTTGATAAATGGGGAAGCTGTATTTCCGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACTGCTCCAACGCAGGCTGTCGCCGTCGGGTTGTGCCGCCAAATCCTGCCACTCATTGAATAACAGCAACAATGTGCCGCCTTCCGACAACGCACCGGCGGCAATAGCGAGGGCATCTAAATGAATGCCTTGGCGCCCATCATAAATGAGCGCGGGGAATTCGCTGCCGAGCAGATT  >  1/1475459‑1475927

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑