Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,475,565	A→G	S171S (TCT→TCC)	unknown ←	tRNA cytosine(34) acetyltransferase (EC 2.3.1.193)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,475,565	0	A	G	100.0%	161.5 / NA	59	S171S (TCT→TCC)	unknown	tRNA cytosine(34) acetyltransferase (EC 2.3.1.193)
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (33/26);  total (33/26)

CCACAAATAACCACGAATCCGCAAATTGCTGTGGATTTTCACCAACCTTTTGGCGCAGTTCATCAGGTGGCATAAAGGGGATTTCGACACCGGAAAATTCATTGAAAATCTTGACCGCACTTTTGTTCGGCGCCGTCAATAGAACGGGCTTATTTGCCGCTAATTGCTGTTTGGCGAACAAGCCCGCCAAAGCGGATTTTCCCCGCCCGCGCTTTGCCGTAACGATTAACATCTCCGCGTCAGATGTCGCCATTTGCCGCAATAAATGGGCTTGTTCCAAGGTCGGTTGGTGTTGTTCTGAAAGCGACTTTTCAATCTTAGGGAAAACGAGCGCAGACGGCAAATGTTGATAAATGGGGAAGCTGTATTTCCGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACTGCTCCAACGCAGGCTGTCGCCGTCGGGTTGTGCCGCCAAATC  >  1/1475324‑1475795                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                       ccACAAATAACCATAAATCCGCAAATTGCTGCGGATTTTCACCAATCTTTCGACGCAGTTCATCAGGCGATATAAACGGGATTTCCGCAGCGGCAAATTCATTGAAAATATTGACCGCACTTTTGTTCAGCGCCGTCAATAGAACAGGCTGATTTGCTTCCAATTGCTGCTTGGCGAACAAGCCTGCCAAAGCGGATTTGCCCCGCCCACGCTTTGCCGTAACGATTAACATCTCTGCACCGGATGTCGcc                                                                                                                                                                                                                               <  1:68449/251‑1 (MQ=255)      aaTAACCATAAATCCGCAAATTGCTGCGGATTTTCACCAATCTTTCGACGCAGTTCATCAGGCGATATAAACGGGATTTCCGCAGCGGCAAATTCATTGAAAATATTGACCGCACTTTTGTTCAGCGCCGTCAATAGAACAGGCTGATTTGCTTCCAATTGCTGCTTGGCGAACAAGCCTGCCAAAGCGGATTTGCCCCGCCCACGCTTTGCCGTAACGATTAACATCTCTGCACCGGATGTCGCCATTTg                                                                                                                                                                                                                          >  1:834540/1‑251 (MQ=255)                    cGCAAATTGCTGCGGATTTTCACCAATCTTTCGACGCAGTTCATCAGGCGATATAAACGGGATTTCCGCAGCGGCAAATTCATTGAAAATATTGACCGCACTTTTGTTCAGCGCCGTCAATAGAACAGGCTGATTTGCTTCCAATTGCTGCTTGGCGAACAAGCCTGCCAAAGCGGATTTGCCCCGCCCACGCTTTGCCGTAACGATTAACATCTCTGCACCGGATGTCGCCATTTGCCGCAATAAATggg                                                                                                                                                                                                            <  1:897422/251‑1 (MQ=255)                    cGCAAATTGCTGCGGATTTTCACCAATCTTTCGACGCAGTTCATCAGGCGATATAAACGGGATTTCCGCAGCGGCAAATTCATTGAAAATATTGACCGCACTTTTGTTCAGCGCCGTCAATAGAACAGGCTGATTTGCTTCCAATTGCTGCTTGGCGAACAAGCCTGCCAAAGCGGATTTGCCCCGCCCACGCTTTGCCGTAACGATTAACATCTCTGCACCGGATGTCGCCATTTGCCGCAATAAATggg                                                                                                                                                                                                            <  1:272634/251‑1 (MQ=255)                          ttGCTGCGGATTTTCACCAATCTTTCGACGCAGTTCATCAGGCGATATAAACGGGATTTCCGCAGCGGCAAATTCATTGAAAATATTGACCGCACTTTTGTTCAGCGCCGTCAATAGAACAGGCTGATTTGCTTCCAATTGCTGCTTGGCGAACAAGCCTGCCAAAGCGGATTTGCCCCGCCCACGCTTTGCCGTAACGATTAACATCTCTGCACCGGATGTCGCCATTTGCCGCAATAAATGGGTTTGtt                                                                                                                                                                                                      >  1:14588/1‑251 (MQ=255)                                cggATTTTCACCAATCTTTCGACGCAGTTCATCAGGCGATATAAACGGGATTTCCGCAGCGGCAAATTCATTGAAAATATTGACCGCACTTTTGTTCAGCGCCGTCAATAGAACAGGCTGATTTGCTTCCAATTGCTGCTTGGCGAACAAGCCTGCCAAAGCGGATTTGCCCCGCCCACGCTTTGCCGTAACGATTAACATCTCTGCACCGGATGTCGCCATTTGCCGCAATAAATGGGTTTGTTCCAAgg                                                                                                                                                                                                <  1:450363/250‑1 (MQ=255)                                          ccaatctttcgacgcAGTTCATCAGGCGATATAAACGGGATTTCCGCAGCGGCAAATTCATTGAAAATATTGACCGCACTTTTGTTCAGCGCCGTCAATAGAACAGGCTGATTTGCTTCCAATTGCTGCTTGGCGAACAAGCCTGCCAAAGCGGATTTGCCCCGCCCACGCTTTGCCGTAACGATTAACATCTCTGCACCGGATGTCGCCATTTGCCGCAATAAATGGGTTTGTTCCAAGGTCGGTTgatg                                                                                                                                                                                      <  1:536541/239‑4 (MQ=255)                                           caatctttcgacgcAGTTCATCAGGCGATATAAACGGGATTTCCGCAGCGGCAAATTCATTGAAAATATTGACCGCACTTTTGTTCAGCGCCGTCAATAGAACAGGCTGATTTGCTTCCAATTGCTGCTTGGCGAACAAGCCTGCCAAAGCGGATTTGCCCCGCCCACGCTTTGCCGTAACGATTAACATCTCTGCACCGGATGTCGCCATTTGCCGCAATAAATGGGTTTGTTCCAAGGTCGGTTGatgt                                                                                                                                                                                     <  1:529655/240‑1 (MQ=255)                                                tttcgacgcAGTTCATCAGGCGATATAAACGGGATTTCCGCAGCGGCAAATTCATTGAAAATATTGACCGCACTTTTGTTCAGCGCCGTCAATAGAACAGGCTGATTTGCTTCCAATTGCTGCTTGGCGAACAAGCCTGCCAAAGCGGATTTGCCCCGCCCACGCTTTGCCGTAACGATTAACATCTCTGCACCGGATGTCGCCATTTGCCGCAATAAATGGGTTTGTTCCAAGGTCGGTTGATgtagtt                                                                                                                                                                                 >  1:536029/7‑250 (MQ=255)                                                     acgcAGTTCATCAGGCGATATAAACGGGATTTCCGCAGCGGCAAATTCATTGAAAATATTGACCGCACTTTTGTTCAGCGCCGTCAATAGAACAGGCTGATTTGCTTCCAATTGCTGCTTGGCGAACAAGCCTGCCAAAGCGGATTTGCCCCGCCCACGCTTTGCCGTAACGATTAACATCTCTGCACCGGATGTCGCCATTTGCCGCAATAAATGGGTTTGTTCCAAGGTCGGTTGATGTAGTTCTGaa                                                                                                                                                                            <  1:832531/249‑1 (MQ=255)                                                      cgcAGTTCATCAGGCGATATAAACGGGATTTCCGCAGCGGCAAATTCATTGAAAATATTGACCGCACTTTTGTTCAGCGCCGTCAATAGAACAGGCTGATTTGCTTCCAATTGCTGCTTGGCGAACAAGCCTGCCAAAGCGGATTTGCCCCGCCCACGCTTTGCCGTAACGATTAACATCTCTGCACCGGATGTCGCCATTTGCCGCAATAAATGGGTTTGTTCCAAGGTCGGTTGATGTAGTTCTGAAAg                                                                                                                                                                          <  1:579153/251‑1 (MQ=255)                                                          gTTCATCAGGCGATATAAACGGGATTTCCGCAGCGGCAAATTCATTGAAAATATTGACCGCACTTTTGTTCAGCGCCGTCAATAGAACAGGCTGATTTGCTTCCAATTGCTGCTTGGCGAACAAGCCTGCCAAAGCGGATTTGCCCCGCCCACGCTTTGCCGTAACGATTAACATCTCTGCACCGGATGTCGCCATTTGCCGCAATAAATGGGTTTGTTCCAAGGTCGGTTGATGTAGTTCTGAAAGCGa                                                                                                                                                                       >  1:177452/1‑250 (MQ=255)                                                          gTTCATCAGGCGATATAAACGGGATTTCCGCAGCGGCAAATTCATTGAAAATATTGACCGCACTTTTGTTCAGCGCCGTCAATAGAACAGGCTGATTTGCTTCCAATTGCTGCTTGGCGAACAAGCCTGCCAAAGCGGATTTGCCCCGCCCACGCTTTGCCGTAACGATTAACATCTCTGCACCGGATGTCGCCATTTGCCGCAATAAATGGGTTTGTTCCAAGGTCGGTTGATGTAGTTCTGAAAGCGa                                                                                                                                                                       >  1:860768/1‑250 (MQ=255)                                                           ttCATCAGGCGATATAAACGGGATTTCCGCAGCGGCAAATTCATTGAAAATATTGACCGCACTTTTGTTCAGCGCCGTCAATAGAACAGGCTGATTTGCTTCCAATTGCTGCTTGGCGAACAAGCCTGCCAAAGCGGATTTGCCCCGCCCACGCTTTGCCGTAACGATTAACATCTCTGCACCGGATGTCGCCATTTGCCGCAATAAATGGGTTTGTTCCAAGGTCGGTTGATGTAGTTCTGAAAGCGAc                                                                                                                                                                      <  1:77978/250‑1 (MQ=255)                                                                      ataTAAACGGGATTTCCGCAGCGGCAAATTCATTGAAAATATTGACCGCACTTTTGTTCAGCGCCGTCAATAGAACAGGCTGATTTGCTTCCAATTGCTGCTTGGCGAACAAGCCTGCCAAAGCGGATTTGCCCCGCCCACGCTTTGCCGTAACGATTAACATCTCTGCACCGGATGTCGCCATTTGCCGCAATAAATGGGTTTGTTc                                                                                                                                                                                                     <  1:418508/206‑1 (MQ=255)                                                                          aaacgggATTTCCGCAGCGGCAAATTCATTGAAAATATTGACCGCACTTTTGTTCAGCGCCGTCAATAGAACAGGCTGATTTGCTTCCAATTGCTGCTTGGCGAACAAGCCTGCCAAAGCGGATTTGCCCCGCCCACGCTTTGCCGTAACGATTAACATCTCTGCACCGGATGTCGCCATTTGCCGCAATAAATGGGTTTGTTCCAAGGTCGGTTGATGTAGTTCTGAAAGCGACGTTTCAATCCTAGGGa                                                                                                                                                      >  1:891812/5‑251 (MQ=255)                                                                               ggatttccgcagcggcaaaTTCATTGAAAATATTGACCGCACTTTTGTTCAGCGCCGTCAATAGAACAGGCTGATTTGCTTCCAATTGCTGCTTGGCGAACAAGCCTGCCAAAGCGGATTTGCCCCGCCCACGCTTTGCCGTAACGATTAACATCTCTGCACCGGATGTCGCCATTTGCCGCAATAAATGGGTTTGTTCCAAGGTCGGTTGATGTAGTTCTGAAAGCGACGTTTCAATcc                                                                                                                                                            >  1:837591/17‑239 (MQ=255)                                                                               ggatttccgcagcggcaaaTTCATTGAAAATATTGACCGCACTTTTGTTCAGCGCCGTCAATAGAACAGGCTGATTTGCTTCCAATTGCTGCTTGGCGAACAAGCCTGCCAAAGCGGATTTGCCCCGCCCACGCTTTGCCGTAACGATTAACATCTCTGCACCGGATGTCGCCATTTGCCGCAATAAATGGGTTTGTTCCAAGGTCGGTTGATGTAGTTCTGAAAGCGACGTTTCAATCCTAGGGaagac                                                                                                                                                  >  1:641658/17‑247 (MQ=255)                                                                                    tccgcagcggcaaaTTCATTGAAAATATTGACCGCACTTTTGTTCAGCGCCGTCAATAGAACAGGCTGATTTGCTTCCAATTGCTGCTTGGCGAACAAGCCTGCCAAAGCGGATTTGCCCCGCCCACGCTTTGCCGTAACGATTAACATCTCTGCACCGGATGTCGCCATTTGCCGCAATAAATGGGTTTGTTCCAAGGTCGGTTGATGTAGTTCTGAAAGCGACGTTTCAATCCTAGGGAAGACCAgcgc                                                                                                                                            <  1:853880/240‑1 (MQ=255)                                                                                              caaaTTCATTGAAAATATTGACCGCACTTTTGTTCAGCGCCGTCAATAGAACAGGCTGATTTGCTTCCAATTGCTGCTTGGCGAACAAGCCTGCCAAAGCGGATTTGCCCCGCCCACGCTTTGCCGTAACGATTAACATCTCTGCACCGGATGTCGCCATTTGCCGCAATAAATGGGTTTGTTCCAAGGTCGGTTGATGTAGTTCTGAAAGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCaga                                                                                                                                  <  1:791685/250‑3 (MQ=255)                                                                                                       tGAAAATATTGACCGCACTTTTGTTCAGCGCCGTCAATAGAACAGGCTGATTTGCTTCCAATTGCTGCTTGGCGAACAAGCCTGCCAAAGCGGATTTGCCCCGCCCACGCTTTGCCGTAACGATTAACATCTCTGCACCGGATGTCGCCATTTGCCGCAATAAATGGGTTTGTTCCAAGGTCGGTTGATGTAGTTCTGAAAGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATaa                                                                                                                         <  1:795066/251‑1 (MQ=255)                                                                                                        gAAAATATTGACCGCACTTTTGTTCAGCGCCGTCAATAGAACAGGCTGATTTGCTTCCAATTGCTGCTTGGCGAACAAGCCTGCCAAAGCGGATTTGCCCCGCCCACGCTTTGCCGTAACGATTAACATCTCTGCACCGGATGTCGCCATTTGCCGCAATAAATGGGTTTGTTCCAAGGTCGGTTGATGTAGTTCTGAAAGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATaaa                                                                                                                        >  1:116385/1‑251 (MQ=255)                                                                                                         aaaaTATTGACCGCACTTTTGTTCAGCGCCGTCAATAGAACAGGCTGATTTGCTTCCAATTGCTGCTTGGCGAACAAGCCTGCCAAAGCGGATTTGCCCCGCCCACGCTTTGCCGTAACGATTAACATCTCTGCACCGGATGTCGCCATTTGCCGCAATAAATGGGTTTGTTCCAAGGTCGGTTGATGTAGTTCTGAAAGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAAt                                                                                                                       >  1:128232/1‑251 (MQ=255)                                                                                                         aaaaTATTGACCGCACTTTTGTTCAGCGCCGTCAATAGAACAGGCTGATTTGCTTCCAATTGCTGCTTGGCGAACAAGCCTGCCAAAGCGGATTTGCCCCGCCCACGCTTTGCCGTAACGATTAACATCTCTGCACCGGATGTCGCCATTTGCCGCAATAAATGGGTTTGTTCCAAGGTCGGTTGATGTAGTTCTGAAAGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAAt                                                                                                                       >  1:246325/1‑251 (MQ=255)                                                                                                                        cTTTTGTTCAGCGCCGTCAATAGAACAGGCTGATTTGCTTCCAATTGCTGCTTGGCGAACAAGCCTGCCAAAGCGGATTTGCCCCGCCCACGCTTTGCCGTAACGATTAACATCTCTGCACCGGATGTCGCCATTTGCCGCAATAAATgggtt                                                                                                                                                                                                          >  1:724398/1‑151 (MQ=255)                                                                                                                                     ccGTCAATAGAACAGGCTGATTTGCTTCCAATTGCTGCTTGGCGAACAAGCCTGCCAAAGCGGATTTGCCCCGCCCACGCTTTGCCGTAACGATTAACATCTCTGCACCGGATGTCGCCATTTGCCGCAATAAATGGGTTTGTTCCAAGGTCGGTTGATGTAGTTCTGAAAGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGAt                                                                                                                           <  1:629920/219‑1 (MQ=255)                                                                                                                                       gTCAATAGAACAGGCTGATTTGCTTCCAATTGCTGCTTGGCGAACAAGCCTGCCAAAGCGGATTTGCCCCGCCCACGCTTTGCCGTAACGATTAACATCTCTGCACCGGATGTCGCCATTTGCCGCAATAAATGGGTTTGTTCCAAGGTCGGTTGATGTAGTTCTGAAAGCGACGTTTCAATcc                                                                                                                                                            >  1:613195/1‑183 (MQ=255)                                                                                                                                        tCAATAGAACAGGCTGATTTGCTTCCAATTGCTGCTTGGCGAACAAGCCTGCCAAAGCGGATTTGCCCCGCCCACGCTTTGCCGTAACGATTAACATCTCTGCACCGGATGTCGCCATTTGCCGCAATAAATGGGTTTGTTCCAAGGTCGGTTGATGTAGTTCTGAAAGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGc                                                                                        <  1:324372/251‑1 (MQ=255)                                                                                                                                         cAATAGAACAGGCTGATTTGCTTCCAATTGCTGCTTGGCGAACAAGCCTGCCAAAGCGGATTTGCCCCGCCCACGCTTTGTCGTAACGATTAACATCTCTGCACCGGATGTCGCCATTTGCCGCAATAAATGGGTTTGTTCCAAGGTCGGTTGATGTAGTTCTGAAAg                                                                                                                                                                          <  1:71132/168‑1 (MQ=255)                                                                                                                                         cAATAGAACAGGCTGATTTGCTTCCAATTGCTGCTTGGCGAACAAGCCTGCCAAAGCGGATTTGCCCCGCCCACGCTTTGCCGTAACGATTAACATCTCTGCACCGGATGTCGCCATTTGCCGCAATAAATGGGTTTGTTCCAAGGt                                                                                                                                                                                               <  1:645381/147‑1 (MQ=255)                                                                                                                                         cAATAGAACAGGCTGATTTGCTTCCAATTGCTGCTTGGCGAACAAGCCTGCCAAAGCGGATTTGCCCCGCCCACGCTTTGCCGTAACGATTAACATCTCTGCACCGGATGTCGCCATTTGCCGCAATAAATGGGTTTGTTCCAAGGTCGGTTGATGTAGTTCTGAAAGCGACGTTTCAATCCTAggg                                                                                                                                                       <  1:471769/187‑1 (MQ=255)                                                                                                                                         cAATAGAACAGGCTGATTTGCTTCCAATTGCTGCTTGGCGAACAAGCCTGCCAAAGCGGATTTGCCCCGCCCACGCTTTGCCGTAACGATTAACATCTCTGCACCGGATGTCGCCATTTGCCGCAATAAATGGGTTTGTTCCAAGGTCGGTTGATGTAGTTCTGAAAGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATggg                                                                                                                    <  1:782676/222‑1 (MQ=255)                                                                                                                                         cAATAGAACAGGCTGATTTGCTTCCAATTGCTGCTTGGCGAACAAGCCTGCCAAAGCGGATTTGCCCCGCCCACGCTTTGCCGTAACGATTAACATCTCTGCACCGGATGTCGCCATTTGCCGCAATAAATGGGTTTGTTCCAAGGTCGGTTGATGTAGTTCTGAAAGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCa                                                                                       >  1:578806/1‑251 (MQ=255)                                                                                                                                         cAATAGAACAGGCTGATTTGCTTCCAATTGCTGCTTGGCGAACAAGCCTGCCAAAGCGGATTTGCCCCGCCCACGCTTTGCCGTAACGATTAACATCTCTGCACCGGATGTCGCCATTTGCCGCAATAAATGGGTTTGTTCCAAGGTCGGTTGATGTAGTTCTGAAAGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCa                                                                                       >  1:471226/1‑251 (MQ=255)                                                                                                                                         cAATAGAACAGGCTGATTTGCTTCCAATTGCTGCTTGGCGAACAAGCCTGCCAAAGCGGATTTGCCCCGCCCACGCTTTGCCGTAACGATTAACATCTCTGCACCGGATGTCGCCATTTGCCGCAATAAATGGGTTTGTTCCAAGGTCGGTTGATGTAGTTCTGAAAGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCa                                                                                       >  1:396196/1‑251 (MQ=255)                                                                                                                                         cAATAGAACAGGCTGATTTGCTTCCAATTGCTGCTTGGCGAACAAGCCTGCCAAAGCGGATTTGCCCCGCCCACGCTTTGCCGTAACGATTAACATCTCTGCACCGGATGTCGCCATTTGCCGCAATAAATGGGTTTGTTCCAAGGTCGGTTGATGTAGTTCTGAAAGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCa                                                                                       >  1:65902/1‑251 (MQ=255)                                                                                                                                         cAATAGAACAGGCTGATTTGCTTCCAATTGCTGCTTGGCGAACAAGCCTGCCAAAGCGGATTTGCCCCGCCCACGCTTTGCCGTAACGATTAACATCTCTGCACCGGATGTCGCCATTTGCCGCAATAAATGGGTTTGTTCCAAGGTCGGTTGATGTAGTTCTGAAAGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCa                                                                                       >  1:660618/1‑251 (MQ=255)                                                                                                                                           aTAGAACAGGCTGATTTGCTTCCAATTGCTGCTTGGCGAACAAGCCTGCCAAAGCGGATTTGCCCCGCCCACGCTTTGCCGTAACGATTAACATCTCTGCACCGGATGTCGCCATTTGCCGCAATAAATGGGTTTGTTCCAAGGTCGGTTGATGTAGTTCTGAAAGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAAt                                                                                                                       >  1:89370/1‑217 (MQ=255)                                                                                                                                              gaacaggctgatttgcttccaaTTGCTGCTTGGCGAACAAGCCTGCCAAAGCGGATTTGCCCCGCCCACGCTTTGCCGTAACGATTAACATCTCTGCACCGGATGTCGCCATTTGCCGCAATAAATGGGTTTGTTCCAAGGTCGGTTGATGTAGTTCTGAAAGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAg                                                                                  >  1:204357/21‑251 (MQ=255)                                                                                                                                              gaacaggctgatttgcttccaaTTGCTGCTTGGCGAACAAGCCTGCCAAAGCGGATTTGCCCCGCCCACGCTTTGCCGTAACGATTAACATCTCTGCACCGGATGTCGCCATTTGCCGCAATAAATGGGTTTGTTCCAAGGTCGGTTGATGTAGTTCTGAAAGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAg                                                                                  >  1:515335/21‑251 (MQ=255)                                                                                                                                                   ggctgatttgcttccaaTTGCTGCTTGGCGAACAAGCCTGCCAAAGCGGATTTGCCCCGCCCACGCTTTGCCGTAACGATTAACATCTCTGCACCGGATGTCGCCATTTGCCGCAATAAATGGGTTTGTTCCAAGGTCGGTTGATGTAGTTCTGAAAGCGACGTTTCAATCCTAggg                                                                                                                                                       >  1:48300/16‑177 (MQ=255)                                                                                                                                                            gcttccaaTTGCTGCTTGGCGAACAAGCCTGCCAAAGCGGATTTGCCCCGCCCACGCTTTGCCGTAACGATTAACATCTCTGCACCGGATGTCGCCATTTGCCGCAATAAATGGGTTTGTTCCAAGGTCGGTTGATGTAGTTCTGAAAGCGACGTTTCAATcc                                                                                                                                                            >  1:335158/7‑162 (MQ=255)                                                                                                                                                                   aTTGCTGCTTGGCGAACAAGCCTGCCAAAGCGGATTTGCCCCGCCCACGCTTTGCCGTAACGATTAACATCTCTGCACCGGATGTCGCCATTTGCCGCAATAAATGGGTTTGTTCCAAGGTCGGTTGATGTAGTTCTGAAAGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGttt                                                             <  1:738563/251‑1 (MQ=255)                                                                                                                                                                      gctgctTGGCGAACAAGCCTGCCAAAGCGGATTTGCCCCGCCCACGCTTTGCCGTAACGATTAACATCTCTGCACCGGATGTCGCCATTTGCCGCAATAAATGGGTTTGTTCCAAGGTCGGTTGATGTAGTTCTGAAAGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTAt                                                           >  1:669075/1‑250 (MQ=255)                                                                                                                                                                       ctgcttGGCGAACAAGCCTGCCAAAGCGGATTTGCCCCGCCCACGCTTTGCCGTAACGATTAACATCTCTGCACCGGATGTCGCCATTTGCCGCAATAAATGGGTTTGTTCCAAGGTCGGTTGATGTAGTTCTAAAAGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATc                                                          <  1:463983/246‑1 (MQ=255)                                                                                                                                                                          cttGGCGAACAAGCCTGCCAAAGCGGATTTGCCCCGCCCACGCTTTGCCGTAACGATTAACATCTCTGCACCGGATGTCGCCATTTGCCGCAATAAATGGGTTTGTTCCAAGGTCGGTTGATGTAGTTCTGAAAGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCggg                                                       <  1:518565/249‑1 (MQ=255)                                                                                                                                                                                gAACAAGCCTGCCAAAGCGGATTTGCCCCGCCCACGCTTTGCCGTAACGATTAACATCTCTGCACCGGATGTCGCCATTTGCCGCAATAAATGGGTTTGTTCCAAGGTCGGTTGATGTAGTTCTGAAAGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGttttt                                                >  1:104312/1‑251 (MQ=255)                                                                                                                                                                                     aGCCTGCCAAAGCGGATTTGCCCCGCCCACGCTTTGCCGTAACGATTAACATCTCTGCACCGGATGTCGCCATTTGCCGCAATAAATGGGTTTGTTCCAAGGTCGGTTGATGTAGTTCTGAAAGCGACGTTTCACTCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCAccg                                           >  1:718452/1‑249 (MQ=255)                                                                                                                                                                                          gCCAAAGCGGATTTGCCCCGCCCACGCTTTGCCGTAACGATTAACATCTCTGCACCGGATGTCGCCATTTGCCGCAATAAATGGGTTTGTTCCAAGGTCGGTTGATGTAGTTCTGAAAGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGAGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCa                                      >  1:582660/1‑251 (MQ=255)                                                                                                                                                                                              aaGCGGATTTGCCCCGCCCACGCTTTGCCGTAACGATTAACATCTCTGCACCGGATGTCGCCATTTGCCGCAATAAATGGGTTTGTTCCAAGGTCGGTTGATGTAGTTCTGAAAGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACg                                   >  1:478547/1‑250 (MQ=255)                                                                                                                                                                                                   gATTTGCCCCGCCCACGCTTTGCCGTAACGATTAACATCTCTGCACCGGATGTCGCCATTTGCCGCAATAAATGGGTTTGTTCCAAGGTCGGTTGATGTAGTTCTGAAAGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCaagc                              <  1:639360/250‑4 (MQ=255)                                                                                                                                                                                                   gATTTGCCCCGCCCACGCTTTGCCGTAACGATTAACATCTCTGCACCGGATGTCGCCATTTGCCGCAATAAATGGGTTTGTTCCAAGGTCGGTTGATGTAGTTCTGAAAGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCaag                               >  1:296060/1‑247 (MQ=255)                                                                                                                                                                                                   gATTTGCCCCGCCCACGCTTTGCCGTAACGATTAACATCTCTGCACCGGATGTCGCCATTTGCCGCAATAAATGGGTTTGTTCCAAGGTCGGTTGATGTAGTTCTGAAAGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCaag                               >  1:241184/1‑247 (MQ=255)                                                                                                                                                                                                                        gCCGTAACGATTAACATCTCTGCACCGGATGTCGCCATTTGCCGCAATAAATGGGTTTGTTCCAAGGTCGGTTGATGTAGTTCTGAAAGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATgg                                                                                                                     >  1:31774/1‑142 (MQ=255)                                                                                                                                                                                                                              aCGATTAACATCTCTGCACCGGATGTCGCCATTTGCCGCAATAAATGGGTTTGTTCCAAGGTCGGTTGATGTAGTTCTGAAAGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATc  <  1:780503/251‑1 (MQ=255)                                                                                                                                                                                                                                gATTAACATCTCTGCACCGGATGTCGCCATTTGCCGCAATAAATGGGTTTGTTCCAAGGTCGGTTGATGTAGTTCTGAAAGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTCACCATCAGGTTGCACCGCCAAATc  >  1:707442/1‑249 (MQ=255)                                                                                                                                                                                                                                 aTTAACATCTCTGCACCGGATGTCGCCATTTGCCGCAATAAATGGGTTTGTTCCAAGGTCGGTTGATGTAGTTCTGAAAGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGAt                                                                                                                           <  1:295264/127‑1 (MQ=255)                                                                                                                                                                                                                                 aTTAACATCTCTGCACCGGATGTCGCCATTTGCCGCAATAAATGGGTTTGTTCCAAGGTCGGTTGATGTAGTTCTGAAAGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGc                                  >  1:171207/1‑216 (MQ=255)                                                                                                                                                                                                                                 aTTAACATCTCTGCACCGGATGTCGCCATTTGCCGCAATAAATGGGTTTGTTCCAAGGTCGGTTGATGTAGTTCTGAAAGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAATGGGAAAGCCGTATTTTTGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACCGCTCCAACGCAAGCTGTcaccatcaggt                <  1:539034/234‑11 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                       CCACAAATAACCACGAATCCGCAAATTGCTGTGGATTTTCACCAACCTTTTGGCGCAGTTCATCAGGTGGCATAAAGGGGATTTCGACACCGGAAAATTCATTGAAAATCTTGACCGCACTTTTGTTCGGCGCCGTCAATAGAACGGGCTTATTTGCCGCTAATTGCTGTTTGGCGAACAAGCCCGCCAAAGCGGATTTTCCCCGCCCGCGCTTTGCCGTAACGATTAACATCTCCGCGTCAGATGTCGCCATTTGCCGCAATAAATGGGCTTGTTCCAAGGTCGGTTGGTGTTGTTCTGAAAGCGACTTTTCAATCTTAGGGAAAACGAGCGCAGACGGCAAATGTTGATAAATGGGGAAGCTGTATTTCCGGATTTTTTCTTGCAGAAAGGCAATAAAGTGCGGTGTGTTTATCGGGTGTTTTTCACTGCTCCAACGCAGGCTGTCGCCGTCGGGTTGTGCCGCCAAATC  >  1/1475324‑1475795

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑