Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,475,452	G→A	P209L (CCG→CTG)	unknown ←	tRNA cytosine(34) acetyltransferase (EC 2.3.1.193)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,475,452	0	G	A	100.0%	72.2 / NA	28	P209L (CCG→CTG)	unknown	tRNA cytosine(34) acetyltransferase (EC 2.3.1.193)
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/15);  total (13/15)

ATCATCGCCGCTTCGTCCACAAATAACCACGAATCCGCAAATTGCTGTGGATTTTCACCAACCTTTTGGCGCAGTTCATCAGGTGGCATAAAGGGGATTTCGACACCGGAAAATTCATTGAAAATCTTGACCGCACTTTTGTTCGGCGCCGTCAATAGAACGGGCTTATTTGCCGCTAATTGCTGTTTGGCGAACAAGCCCGCCAAAGCGGATTTTCCCCGCCCGCGCTTTGCCGTAACGATTAACATCTCCGCGTCAGATGTCGCCATTTGCCGCAATAAATGGGCTTGTTCCAAGGTCGGTTGGTGTTGTTCTGAAAGCGACTTTTCAATCTTAGGGAAAACGAGCGCAGACGGCAAATGTTGATAAAT  >  1/1475308‑1475678                                                                                                                                                 |                                                                                                                                                                                                                                   atcatcGCCGCTTCATCCACAAATAACCATAAATCCGCAAATTGCTGCGGATTTTCACCAATCTTTCGACGCAGTTCATCAGGCGATATAAACGGGATTTCCGCAGCGGCAAATTCATTGAAAATATTGACCGCACTTTTGTTCAGCGCCGTCAATAGAACAGGCTGATTTGCTTCCAATTGCTGCTTGGCGAACAAGCCTGCCAAAGCGGATTTGCCCCGCCCACGCTTTGCCGTAACGATTAACAtctc                                                                                                                          <  1:694667/251‑1 (MQ=255)         cgcTTCATCCACAAATAACCATAAATCCGCAAATTGCTGCGGATTTTCACCAATCTTTCGACGCAGTTCATCAGGCGATATAAACGGGATTTCCGCAGCGGCAAATTCATTGAAAATATTGACCGCACTTTTGTTCAGCGCCGTCAATAGAACAGGCTGATTTGCTTCCAATTGCTGCTTGGCGAACAAGCCTGCCAAAGCGGATTTGCCCCGCCCACGCTTTGCCGTAACGATTAACAtctctgcaccgg                                                                                                                  <  1:443875/251‑9 (MQ=255)          gcTTCATCCACAAATAACCATAAATCCGCAAATTGCTGCGGATTTTCACCAATCTTTCGACGCAGTTCATCAGGCGATATAAACGGGATTTCCGCAGCGGCAAATTCATTGAAAATATTGACCGCACTTTTGTTCAGCGCCGTCAATAGAACAGGCTGATTTGCTTCCAATTGCTGCTTGGCGAACAAGCCTGCCAAAGCGGATTTGCCCCGCCCACGCTTTGCCGTAACGATTAACAtctctgcaccgga                                                                                                                 >  1:278547/1‑242 (MQ=255)                 ccACAAATAACCATAAATCCGCAAATTGCTGCGGATTTTCACCAATCTTTCGACGCAGTTCATCAGGCGATATAAACGGGATTTCCGCAGCGGCAAATTCATTGAAAATATTGACCGCACTTTTGTTCAGCGCCGTCAATAGAACAGGCTGATTTGCTTCCAATTGCTGCTTGGCGAACAAGCCTGCCAAAGCGGATTTGCCCCGCCCACGCTTTGCCGTAACGATTAACATCTCTGCACCGGATGTCGcc                                                                                                          <  1:68449/251‑1 (MQ=255)                      aaTAACCATAAATCCGCAAATTGCTGCGGATTTTCACCAATCTTTCGACGCAGTTCATCAGGCGATATAAACGGGATTTCCGCAGCGGCAAATTCATTGAAAATATTGACCGCACTTTTGTTCAGCGCCGTCAATAGAACAGGCTGATTTGCTTCCAATTGCTGCTTGGCGAACAAGCCTGCCAAAGCGGATTTGCCCCGCCCACGCTTTGCCGTAACGATTAACATCTCTGCACCGGATGTCGCCATTTg                                                                                                     >  1:834540/1‑251 (MQ=255)                                    cGCAAATTGCTGCGGATTTTCACCAATCTTTCGACGCAGTTCATCAGGCGATATAAACGGGATTTCCGCAGCGGCAAATTCATTGAAAATATTGACCGCACTTTTGTTCAGCGCCGTCAATAGAACAGGCTGATTTGCTTCCAATTGCTGCTTGGCGAACAAGCCTGCCAAAGCGGATTTGCCCCGCCCACGCTTTGCCGTAACGATTAACATCTCTGCACCGGATGTCGCCATTTGCCGCAATAAATggg                                                                                       <  1:897422/251‑1 (MQ=255)                                    cGCAAATTGCTGCGGATTTTCACCAATCTTTCGACGCAGTTCATCAGGCGATATAAACGGGATTTCCGCAGCGGCAAATTCATTGAAAATATTGACCGCACTTTTGTTCAGCGCCGTCAATAGAACAGGCTGATTTGCTTCCAATTGCTGCTTGGCGAACAAGCCTGCCAAAGCGGATTTGCCCCGCCCACGCTTTGCCGTAACGATTAACATCTCTGCACCGGATGTCGCCATTTGCCGCAATAAATggg                                                                                       <  1:272634/251‑1 (MQ=255)                                          ttGCTGCGGATTTTCACCAATCTTTCGACGCAGTTCATCAGGCGATATAAACGGGATTTCCGCAGCGGCAAATTCATTGAAAATATTGACCGCACTTTTGTTCAGCGCCGTCAATAGAACAGGCTGATTTGCTTCCAATTGCTGCTTGGCGAACAAGCCTGCCAAAGCGGATTTGCCCCGCCCACGCTTTGCCGTAACGATTAACATCTCTGCACCGGATGTCGCCATTTGCCGCAATAAATGGGTTTGtt                                                                                 >  1:14588/1‑251 (MQ=255)                                                cggATTTTCACCAATCTTTCGACGCAGTTCATCAGGCGATATAAACGGGATTTCCGCAGCGGCAAATTCATTGAAAATATTGACCGCACTTTTGTTCAGCGCCGTCAATAGAACAGGCTGATTTGCTTCCAATTGCTGCTTGGCGAACAAGCCTGCCAAAGCGGATTTGCCCCGCCCACGCTTTGCCGTAACGATTAACATCTCTGCACCGGATGTCGCCATTTGCCGCAATAAATGGGTTTGTTCCAAgg                                                                           <  1:450363/250‑1 (MQ=255)                                                          ccaatctttcgacgcAGTTCATCAGGCGATATAAACGGGATTTCCGCAGCGGCAAATTCATTGAAAATATTGACCGCACTTTTGTTCAGCGCCGTCAATAGAACAGGCTGATTTGCTTCCAATTGCTGCTTGGCGAACAAGCCTGCCAAAGCGGATTTGCCCCGCCCACGCTTTGCCGTAACGATTAACATCTCTGCACCGGATGTCGCCATTTGCCGCAATAAATGGGTTTGTTCCAAGGTCGGTTgatg                                                                 <  1:536541/239‑4 (MQ=255)                                                           caatctttcgacgcAGTTCATCAGGCGATATAAACGGGATTTCCGCAGCGGCAAATTCATTGAAAATATTGACCGCACTTTTGTTCAGCGCCGTCAATAGAACAGGCTGATTTGCTTCCAATTGCTGCTTGGCGAACAAGCCTGCCAAAGCGGATTTGCCCCGCCCACGCTTTGCCGTAACGATTAACATCTCTGCACCGGATGTCGCCATTTGCCGCAATAAATGGGTTTGTTCCAAGGTCGGTTGatgt                                                                <  1:529655/240‑1 (MQ=255)                                                                tttcgacgcAGTTCATCAGGCGATATAAACGGGATTTCCGCAGCGGCAAATTCATTGAAAATATTGACCGCACTTTTGTTCAGCGCCGTCAATAGAACAGGCTGATTTGCTTCCAATTGCTGCTTGGCGAACAAGCCTGCCAAAGCGGATTTGCCCCGCCCACGCTTTGCCGTAACGATTAACATCTCTGCACCGGATGTCGCCATTTGCCGCAATAAATGGGTTTGTTCCAAGGTCGGTTGATgtagtt                                                            >  1:536029/7‑250 (MQ=255)                                                                     acgcAGTTCATCAGGCGATATAAACGGGATTTCCGCAGCGGCAAATTCATTGAAAATATTGACCGCACTTTTGTTCAGCGCCGTCAATAGAACAGGCTGATTTGCTTCCAATTGCTGCTTGGCGAACAAGCCTGCCAAAGCGGATTTGCCCCGCCCACGCTTTGCCGTAACGATTAACATCTCTGCACCGGATGTCGCCATTTGCCGCAATAAATGGGTTTGTTCCAAGGTCGGTTGATGTAGTTCTGaa                                                       <  1:832531/249‑1 (MQ=255)                                                                      cgcAGTTCATCAGGCGATATAAACGGGATTTCCGCAGCGGCAAATTCATTGAAAATATTGACCGCACTTTTGTTCAGCGCCGTCAATAGAACAGGCTGATTTGCTTCCAATTGCTGCTTGGCGAACAAGCCTGCCAAAGCGGATTTGCCCCGCCCACGCTTTGCCGTAACGATTAACATCTCTGCACCGGATGTCGCCATTTGCCGCAATAAATGGGTTTGTTCCAAGGTCGGTTGATGTAGTTCTGAAAg                                                     <  1:579153/251‑1 (MQ=255)                                                                          gTTCATCAGGCGATATAAACGGGATTTCCGCAGCGGCAAATTCATTGAAAATATTGACCGCACTTTTGTTCAGCGCCGTCAATAGAACAGGCTGATTTGCTTCCAATTGCTGCTTGGCGAACAAGCCTGCCAAAGCGGATTTGCCCCGCCCACGCTTTGCCGTAACGATTAACATCTCTGCACCGGATGTCGCCATTTGCCGCAATAAATGGGTTTGTTCCAAGGTCGGTTGATGTAGTTCTGAAAGCGa                                                  >  1:860768/1‑250 (MQ=255)                                                                          gTTCATCAGGCGATATAAACGGGATTTCCGCAGCGGCAAATTCATTGAAAATATTGACCGCACTTTTGTTCAGCGCCGTCAATAGAACAGGCTGATTTGCTTCCAATTGCTGCTTGGCGAACAAGCCTGCCAAAGCGGATTTGCCCCGCCCACGCTTTGCCGTAACGATTAACATCTCTGCACCGGATGTCGCCATTTGCCGCAATAAATGGGTTTGTTCCAAGGTCGGTTGATGTAGTTCTGAAAGCGa                                                  >  1:177452/1‑250 (MQ=255)                                                                           ttCATCAGGCGATATAAACGGGATTTCCGCAGCGGCAAATTCATTGAAAATATTGACCGCACTTTTGTTCAGCGCCGTCAATAGAACAGGCTGATTTGCTTCCAATTGCTGCTTGGCGAACAAGCCTGCCAAAGCGGATTTGCCCCGCCCACGCTTTGCCGTAACGATTAACATCTCTGCACCGGATGTCGCCATTTGCCGCAATAAATGGGTTTGTTCCAAGGTCGGTTGATGTAGTTCTGAAAGCGAc                                                 <  1:77978/250‑1 (MQ=255)                                                                                      ataTAAACGGGATTTCCGCAGCGGCAAATTCATTGAAAATATTGACCGCACTTTTGTTCAGCGCCGTCAATAGAACAGGCTGATTTGCTTCCAATTGCTGCTTGGCGAACAAGCCTGCCAAAGCGGATTTGCCCCGCCCACGCTTTGCCGTAACGATTAACATCTCTGCACCGGATGTCGCCATTTGCCGCAATAAATGGGTTTGTTc                                                                                <  1:418508/206‑1 (MQ=255)                                                                                          aaacgggATTTCCGCAGCGGCAAATTCATTGAAAATATTGACCGCACTTTTGTTCAGCGCCGTCAATAGAACAGGCTGATTTGCTTCCAATTGCTGCTTGGCGAACAAGCCTGCCAAAGCGGATTTGCCCCGCCCACGCTTTGCCGTAACGATTAACATCTCTGCACCGGATGTCGCCATTTGCCGCAATAAATGGGTTTGTTCCAAGGTCGGTTGATGTAGTTCTGAAAGCGACGTTTCAATCCTAGGGa                                 >  1:891812/5‑251 (MQ=255)                                                                                               ggatttccgcagcggcaaaTTCATTGAAAATATTGACCGCACTTTTGTTCAGCGCCGTCAATAGAACAGGCTGATTTGCTTCCAATTGCTGCTTGGCGAACAAGCCTGCCAAAGCGGATTTGCCCCGCCCACGCTTTGCCGTAACGATTAACATCTCTGCACCGGATGTCGCCATTTGCCGCAATAAATGGGTTTGTTCCAAGGTCGGTTGATGTAGTTCTGAAAGCGACGTTTCAATcc                                       >  1:837591/17‑239 (MQ=255)                                                                                               ggatttccgcagcggcaaaTTCATTGAAAATATTGACCGCACTTTTGTTCAGCGCCGTCAATAGAACAGGCTGATTTGCTTCCAATTGCTGCTTGGCGAACAAGCCTGCCAAAGCGGATTTGCCCCGCCCACGCTTTGCCGTAACGATTAACATCTCTGCACCGGATGTCGCCATTTGCCGCAATAAATGGGTTTGTTCCAAGGTCGGTTGATGTAGTTCTGAAAGCGACGTTTCAATCCTAGGGaagac                             >  1:641658/17‑247 (MQ=255)                                                                                                    tccgcagcggcaaaTTCATTGAAAATATTGACCGCACTTTTGTTCAGCGCCGTCAATAGAACAGGCTGATTTGCTTCCAATTGCTGCTTGGCGAACAAGCCTGCCAAAGCGGATTTGCCCCGCCCACGCTTTGCCGTAACGATTAACATCTCTGCACCGGATGTCGCCATTTGCCGCAATAAATGGGTTTGTTCCAAGGTCGGTTGATGTAGTTCTGAAAGCGACGTTTCAATCCTAGGGAAGACCAgcgc                       <  1:853880/240‑1 (MQ=255)                                                                                                              caaaTTCATTGAAAATATTGACCGCACTTTTGTTCAGCGCCGTCAATAGAACAGGCTGATTTGCTTCCAATTGCTGCTTGGCGAACAAGCCTGCCAAAGCGGATTTGCCCCGCCCACGCTTTGCCGTAACGATTAACATCTCTGCACCGGATGTCGCCATTTGCCGCAATAAATGGGTTTGTTCCAAGGTCGGTTGATGTAGTTCTGAAAGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCaga             <  1:791685/250‑3 (MQ=255)                                                                                                                       tGAAAATATTGACCGCACTTTTGTTCAGCGCCGTCAATAGAACAGGCTGATTTGCTTCCAATTGCTGCTTGGCGAACAAGCCTGCCAAAGCGGATTTGCCCCGCCCACGCTTTGCCGTAACGATTAACATCTCTGCACCGGATGTCGCCATTTGCCGCAATAAATGGGTTTGTTCCAAGGTCGGTTGATGTAGTTCTGAAAGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATaa    <  1:795066/251‑1 (MQ=255)                                                                                                                        gAAAATATTGACCGCACTTTTGTTCAGCGCCGTCAATAGAACAGGCTGATTTGCTTCCAATTGCTGCTTGGCGAACAAGCCTGCCAAAGCGGATTTGCCCCGCCCACGCTTTGCCGTAACGATTAACATCTCTGCACCGGATGTCGCCATTTGCCGCAATAAATGGGTTTGTTCCAAGGTCGGTTGATGTAGTTCTGAAAGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATaaa   >  1:116385/1‑251 (MQ=255)                                                                                                                         aaaaTATTGACCGCACTTTTGTTCAGCGCCGTCAATAGAACAGGCTGATTTGCTTCCAATTGCTGCTTGGCGAACAAGCCTGCCAAAGCGGATTTGCCCCGCCCACGCTTTGCCGTAACGATTAACATCTCTGCACCGGATGTCGCCATTTGCCGCAATAAATGGGTTTGTTCCAAGGTCGGTTGATGTAGTTCTGAAAGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAAt  >  1:246325/1‑251 (MQ=255)                                                                                                                         aaaaTATTGACCGCACTTTTGTTCAGCGCCGTCAATAGAACAGGCTGATTTGCTTCCAATTGCTGCTTGGCGAACAAGCCTGCCAAAGCGGATTTGCCCCGCCCACGCTTTGCCGTAACGATTAACATCTCTGCACCGGATGTCGCCATTTGCCGCAATAAATGGGTTTGTTCCAAGGTCGGTTGATGTAGTTCTGAAAGCGACGTTTCAATCCTAGGGAAGACCAGCGCGGGCGGCAGATATTGATAAAt  >  1:128232/1‑251 (MQ=255)                                                                                                                                        cTTTTGTTCAGCGCCGTCAATAGAACAGGCTGATTTGCTTCCAATTGCTGCTTGGCGAACAAGCCTGCCAAAGCGGATTTGCCCCGCCCACGCTTTGCCGTAACGATTAACATCTCTGCACCGGATGTCGCCATTTGCCGCAATAAATgggtt                                                                                     >  1:724398/1‑151 (MQ=255)                                                                                                                                                 |                                                                                                                                                                                                                                   ATCATCGCCGCTTCGTCCACAAATAACCACGAATCCGCAAATTGCTGTGGATTTTCACCAACCTTTTGGCGCAGTTCATCAGGTGGCATAAAGGGGATTTCGACACCGGAAAATTCATTGAAAATCTTGACCGCACTTTTGTTCGGCGCCGTCAATAGAACGGGCTTATTTGCCGCTAATTGCTGTTTGGCGAACAAGCCCGCCAAAGCGGATTTTCCCCGCCCGCGCTTTGCCGTAACGATTAACATCTCCGCGTCAGATGTCGCCATTTGCCGCAATAAATGGGCTTGTTCCAAGGTCGGTTGGTGTTGTTCTGAAAGCGACTTTTCAATCTTAGGGAAAACGAGCGCAGACGGCAAATGTTGATAAAT  >  1/1475308‑1475678

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑