Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,475,253	C→G	T275T (ACG→ACC)	unknown ←	tRNA cytosine(34) acetyltransferase (EC 2.3.1.193)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,475,253	0	C	G	100.0%	166.3 / NA	60	T275T (ACG→ACC)	unknown	tRNA cytosine(34) acetyltransferase (EC 2.3.1.193)
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (28/32);  total (28/32)

GCGGAATCTGTTCTTCGTAAGAAATCTGAATCTGTTTGGCAAGGTTGGCATCGTAACGCGGTTGCGTCAGACGATCTTCGCAATCACACAACAACAATTCATCAATAAAGCGCTCCAGTTTGTCATCCGCCTGCCAACGTAACGGGCTGAACAATTCAAAATGCCGCAAAGTGCGGTCGGTTTTCGTCATGAATTTTAACAAGAAGCCCCTGCCCGTGCCTTCGTAGCTGTGAATGGTGGTCGTCAGCACAATACGTTTAAAGGCAGTCGTTAAATCAAACAGCGTATCCAACGGAATCATCGCCGCTTCGTCCACAAATAACCACGAATCCGCAAATTGCTGTGGATTTTCACCAACCTTTTGGCGCAGTTCATCAGGTGGCATAAAGGGGATTTCGACACCGGAAAATTCATTGAAAATCTTGACCGCACTTTTGTTCG  >  1/1475012‑1475452                                                                                                                                                                                                                                                  |                                                                                                                                                                                                        gCGGAATCCGTTCTTTGTAAGAAATCTGAATCTGTTCGGCAAGGTTAGCGTCATAACACGGTTGCGGCAGACGATCTTCGCAATCACACAACAACAATTCGTCAATAAAGCGCTCCAGTTTGTCATCCGCCTGCCAACGTAGCGGGCTGAACAATTCAAAATGCCGAAAAGTGCGGTCGGTGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAATGGTCGTGGTCAGcac                                                                                                                                                                                                 <  1:109686/250‑1 (MQ=255) gCGGAATCCGTTCTTTGTAAGAAATCTGAATCTGTTCGGCAAGGTTAGCGTCATAACACGGTTGCGGCAGACGATCTTCGCAATCACACAACAACAATTCGTCAATAAAGCGCTCCAGTTTGTCATCCGCCTGCCAACGTAGCGGGCTGAACAATTCAAAATGCCGAAAAGTGCGGTCGGTGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAATGGTCGTGGTCAGcac                                                                                                                                                                                                 <  1:328430/250‑1 (MQ=255) gCGGAATCCGTTCTTTGTAAGAAATCTGAATCTGTTCGGCAAGGTTAGCGTCATAACACGGTTGCGGCAGACGATCTTCGCAATCACACAACAACAATTCGTCAATAAAGCGCTCCAGTTTGTCATCCGCCTGCCAACGTAGCGGGCTGAACAATTCAAAATGCCGAAAAGTGCGGTCGGTGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAATGGTCGTGGTCAGcac                                                                                                                                                                                                 <  1:408326/250‑1 (MQ=255) gCGGAATCCGTTCTTTGTAAGAAATCTGAATCTGTTCGGCAAGGTTAGCGTCATAACACGGTTGCGGCAGACGATCTTCGCAATCACACAACAACAATTCGTCAATAAAGCGCTCCAGTTTGTCATCCGCCTGCCAACGTAGCGGGCTGAACAATTCAAAATGCCGAAAAGTGCGGTCGGTGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAATGGTCGTGGTCAGcac                                                                                                                                                                                                 <  1:713065/250‑1 (MQ=255)    gAATCCGTTCTTTGTAAGAAATCTGAATCTGTTCGGCAAGGTTAGCGTCATAACACGGTTGCGGCAGACGATCTTCGCAATCACACAACAACAATTCGTCAATAAAGCGCTCCAGTTTGTCATCCGCCTGCCAACGTAGCGGGCTGAACAATTCAAAATGCCGAAAAGTGCGGTCGGTGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAATGGTCGTGGTCAGCACaa                                                                                                                                                                                               >  1:535326/1‑249 (MQ=255)    gAATCCGTTCTTTGTAAGAAATCTGAATCTGTTCGGCAAGGTTAGCGTCATAACACGGTTGCGGCAGACGATCTTCGCAATCACACAACAACAATTCGTCAATAAAGCGCTCCAGTTTGTCATCCGCCTGCCAACGTAGCGGGCTGAACAATTCAAAATGCCGAAAAGTACGGTCGGTGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAATGGTCGTGGTCAGCACaa                                                                                                                                                                                               >  1:447481/1‑249 (MQ=255)     aaTCCGTTCTTTGTAAGAAATCTGAATCTGTTCGGCAAGGTTAGCGTCATAACACGGTTGCGGCAGACGATCTTCGCAATCACACAACAACAATTCGTCAATAAAGCGCTCCAGTTTGTCATCCGCCTGCCAACGTAGCGGGCTGAACAATTCAAAATGCCGAAAAGTGCGGTCGGTGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAATGGTCGTGGTCAGCACAAtgc                                                                                                                                                                                            <  1:429962/251‑3 (MQ=255)             ctttgTAAGAAATCTGAATCTGTTCGGCAAGGTTAGCGTCATAACACGGTTGCGGCAGACGATCTTCGCAATCACACAACAACAATTCGTCAATAAAGCGCTCCAGTTTGTCATCCGCCTGCCAACGTAGCGGGCTGAACAATTCAAAATGCCGAAAAGTGCGGTCGGTGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAATGGTCGTGGTCAGCACAATGCGTTTAAAg                                                                                                                                                                                    <  1:801365/247‑1 (MQ=255)                                   ttcggCAAGGTTAGCGTCATAACACGGTTGCGGCAGACGATCTTCGCAATCACACAACAACAATTCGTCAATAAAGCGCTCCAGTTTGTCATCCGCCTGCCAACGTAGCGGGCTGAACAATTCAAAATGCCGAAAAGTGCGGTCGGTGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAATGGTCGTGGTCAGCACAATGCGTTTAAAGACGGCAGTTAAAGCAAACAGCg                                                                                                                                                              <  1:595812/248‑1 (MQ=255)                                   ttcggCAAGGTTAGCGTCATAACACGGTTGCGGCAGACGATCTTCGCAATCACACAACAACAATTCGTCAATAAAGCGCTCCAGTTTGTCATCCGCCTGCCAACGTAGCGGGCTGAACAATTCAAAATGCCGAAAAGTGCGGTCGGTGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAATGGTCGTGGTCAGCACAATGCGTTTAAAGACGGCAGTTAAAGCAAACAGCg                                                                                                                                                              <  1:279059/248‑1 (MQ=255)                                       gCAAGGTTAGCGTCATAACACGGTTGCGGCAGACGATCTTCGCAATCACACAACAACAATTCGTCAATAAAGCGCTCCAGTTTGTCATCCGCCTGCCAACGTAGCGGGCTGAACAATTCAAAATGCCGAAAAGTGCGGTCGGTGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAATGGTCGTGGTCAGCACAATGCGTTTAAAGACGGCAGTTAAAGCAAACAGCGTAt                                                                                                                                                           <  1:462804/250‑1 (MQ=255)                                           ggttagcgtcatAACACGGTTGCGGCAGACGATCTTCGCAATCACACAACAACAATTCGTCAATAAAGCGCTCCAGTTTGTCATCCGCCTGCCAACGTAGCGGGCTGAACAATTCAAAATGCCGAAAAGTGCGGTCGGTGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAATGGTCGTGGTCAGCACAATGCGTTTAAAGACGGCAGTTAAAGCAAACAGCGTATCCaa                                                                                                                                                       >  1:386367/12‑250 (MQ=255)                                            gttagcgtcatAACACGGTTGCGGCAGACGATCTTCGCAATCACACAACAACAATTCGTCAATAAAGCGCTCCAGTTTGTCATCCGCCTGCCAACGTAGCGGGCTGAACAATTCAAAATGCCGAAAAGTGCGGTCGGTGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAATGGTCGTGGTCAGCACAATGCGTTTAAAGACGGCAGTTAAAGCAAACAGCGTATCCAAc                                                                                                                                                      <  1:574376/240‑1 (MQ=255)                                                 cgtcatAACACGGTTGCGGCAGACGATCTTCGCAATCACACAACAACAATTCGTCAATAAAGCGCTCCAGTTTGTCATCCGCCTGCCAACGTAGCGGGCTGAACAATTCAAAATGCCGAAAAGTGCGGTCGGTGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAATGGTCGTGGTCAGCACAATGCGTTTAAAGACGGCAGTTAAAGCAAACAGCGTATCCAACgggc                                                                                                                                                  <  1:538881/244‑3 (MQ=255)                                                 cgtcatAACACGGTTGCGGCAGACGATCTTCGCAATCACACAACAACAATTCGTCAATAAAGCGCTCCAGTTTGTCATCCGCCTGCCAACGTAGCGGGCTGAACAATTCAAAATGCCGAAAAGTGCGGTCGGTGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAATGGTCGTGGTCAGCACAATGCGTTTAAAGACGGCAGTTAAAGCAAACAGCGTATCCAACgggat                                                                                                                                                 <  1:481754/245‑4 (MQ=255)                                                   tcatAACACGGTTGCGGCAGACGATCTTCGCAATCACACAACAACAATTCGTCAATAAAGCGCTCCAGTTTGTCATCCGCCTGCCAACGTAGCGGGCTGAACAATTCAAAATGCCGAAAAGTGCGGTCGGTGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAATGGTCGTGGTCAGCACAATGCGTTTAAAGACGGCAGTTAAAGCAAACAGCGTATc                                                                                                                                                          >  1:17517/4‑239 (MQ=255)                                                   tcatAACACGGTTGCGGCAGACGATCTTCGCAATCACACAACAACAATTCGTCAATAAAGCGCTCCAGTTTGTCATCCGCCTGCCAACGTAGCGGGCTGAACAATTCAAAATGCCGAAAAGTGCGGTCGGTGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAATGGTCGTGGTCAGCACAATGCGTTTAAAGACGGCAGTTAAAGCAAACAGCGTATCCAACgggat                                                                                                                                                 <  1:227623/245‑4 (MQ=255)                                                   tcatAACACGGTTGCGGCAGACGATCTTCGCAATCACACAACAACAATTCGTCAATAAAGCGCTCCAGTTTGTCATCCGCCTGCCAACGTAGCGGGCTGAACAATTCAAAATGCCGAAAAGTGCGGTCGGTGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAATGGTCGTGGTCAGCACAATGCGTTTAAAGACGGCAGTTAAAGCAAACAGCGTATCCAACGGGatcat                                                                                                                                              >  1:144935/4‑251 (MQ=255)                                                      tAACACGGTTGCGGCAGACGATCTTCGCAATCACACAACAACAATTCGTCAATAAAGCGCTCCAGTTTGTCATCCGCCTGCCAACGTAGCGGGCTGAACAATTCAAAATGCCGAAAAGTGCGGTCGGTGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAATGGTCGTGGTCAGcac                                                                                                                                                                                                 <  1:887053/197‑1 (MQ=255)                                                      tAACACGGTTGCGGCAGACGATCTTCGCAATCACACAACAACAATTCGTCAATAAAGCGCTCCAGTTTGTCATCCGCCTGCCAACGTAGCGGGCTGAACAATTCAAAATGCCGAAAAGTGCGGTCGGTGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAATGGTCGTGGTCAGCACAATGCGTTTAAAgacggc                                                                                                                                                                               <  1:840581/215‑6 (MQ=255)                                                            ggTTGCGGCAGACGATCTTCGCAATCACACAACAACAATTCGTCAATAAAGCGCTCCAGTTTGTCATCCGCCTGCCAACGTAGCGGGCTGATCAATTCAAAATGCCGAAAAGTGCGGTCGGTGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAATGGTCGTGGTCAGCACAATGCGTTTAAAGACGGCAGTTAAAGCAAACAGCGTATCCAACGGGATCATCGCCGCtt                                                                                                                                      >  1:617817/1‑250 (MQ=255)                                                               tGCGGCAGACGATCTTCGCAATCACACAACAACAATTCGTCAATAAAGCGCTCCAGTTTGTCATCCGCCTGCCAACGTAGCGGGCTGAACAATTCAAAATGCCGAAAAGTGCGGTCGGTGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAATGGTCGTGGTCAGCACAATGCGTTTAAAGACGGCAGTTAAAGCAAACAGCGTATCCAACGGGATCATCGCCGCTTcatc                                                                                                                                  <  1:699861/251‑4 (MQ=255)                                                                          aTCTTCGCAATCACACAACAACAATTCGTCAATAAAGCGCTCCAGTTTGTCATCCGCCTGCCAACGTAGCGGGCTGAACAATTCAAAATGCCGAAAAGTGCGGTCGGTGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAATGGTCGTGGTCAGCACAATGCGTTTAAAGACGGCAGTTAAAGCAAACAGCGTATCCAACGGGATCATCGCCGCTTCATCCACAAATAAcc                                                                                                                       <  1:523360/251‑1 (MQ=255)                                                                                       cacaACAACAATTCGTCAATAAAGCGCTCCAGTTTGTCATCCGCCTGCCAACGTAGCGGGCTGAACAATTCAAAATGCCGAAAAGTGCGGTCGGTGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAATGGTCGTGGTCAGCACAATGCGTTTAAAGACGGCAGTTAAAGCAAACAGCGTATCCAACGGGATCATcgc                                                                                                                                           >  1:218698/1‑218 (MQ=255)                                                                                         caacaacaaTTCGTCAATAAAGCGCTCCAGTTTGTCATCCGCCTGCCAACGTAGCGGGCTGAACAATTCAAAATGCCGAAAAGTGCGGTCGGTGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAATGGTCGTGGTCAGCACAATGCGTTTAAAGACGGCAGTTAAAGCAAACAGCGTATCCAACggga                                                                                                                                                  <  1:778456/209‑3 (MQ=255)                                                                                         caacaacaaTTCGTCAATAAAGCGCTCCAGTTTGTCATCCGCCTGCCAACGTAGCGGGCTGAACAATTCAAAATGCCGAAAAGTGCGGTCGGTGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAATGGTCGTGGTCAGCACAATGCGTTTAAAGACGGCAGTTAAAGCAAACAGCGTATCCAACGGGATCATCGCCGCTTCATCCACAAATAACCATAAATCCGCAAAtt                                                                                                        >  1:772507/1‑251 (MQ=255)                                                                                           acaacaaTTCGTCAATAAAGCGCTCCAGTTTGTCATCCGCCTGCCAACGTAGCGGGCTGAACAATTCAAAATGCCGAAAAGTGCGGTCGGTGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAATGGTCGTGGTCAGCACAATGCGTTTAAAGACGGCAGTTAAAGCAAACAGCGTATCCAACgg                                                                                                                                                    <  1:207128/205‑1 (MQ=255)                                                                                                 aTTCGTCAATAAAGCGCTCCAGTTTGTCATCCGCCTGCCAACGTAGCGGGCTGAACAATTCAAAATGCCGAAAAGTGCGGTCGGTGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAATGGTCGTGGTCAGCACAATGCGTTTAAAGACGGCAGTTAAAGCAAACAGCGTATCCAACGGGATCATCGCCGCTTCATCCACAAATAACCataaatc                                                                                                                >  1:6438/1‑229 (MQ=255)                                                                                                     gtcaATAAAGCGCTCCAGTTTGTCATCCGCCTGCCAACGTAGCGGGCTGAACAATTCAAAATGCCGAAAAGTGCGGTCGGTGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAATGGTCGTGGTCAGCACAATGCGTTTAAAGACGGCAGTTAAAGCAAACAGCGTATccc                                                                                                                                                        >  1:592032/2‑190 (MQ=255)                                                                                                     gtcaATAAAGCGCTCCAGTTTGTCATCCGCCTGCCAACGTAGCGGGCTGAACAATTCAAAATGCCGAAAAGTGCGGTCGGTGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAATGGTCGTGGTCAGCACAATGCGTTTAAAGACGGCAGTTAAAGCAAACAGCGTATCCAACGGGATCATCGCCGCTTCATCCACAAATAACCATAAATCCGCAAATTGCTGCGGAtttt                                                                                            >  1:86345/2‑251 (MQ=255)                                                                                                      tcaATAAAGCGCTCCAGTTTGTCATCCGCCTGCCAACGTAGCGGGCTGAACAATTCAAAATGCCGAAAAGTGCGGTCGGTGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAATGGTCGTGGTCAGCACAATGCGTTTAAAGACGGCAGTTAAAGCAAACAGCGTATCCAACGGGATCATCGCCGCTTCATCCACAAATAACCATAAATCCGCAAATTGCTGCGGAtttt                                                                                            >  1:443041/1‑250 (MQ=255)                                                                                                         aTAAAGCGCTCCAGTTTGTCATCCGCCTGCCAACGTAGCGGGCTGAACAATTCAAAATGCCGAAAAGTGCGGTCGGTGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAATGGTCGTGGTCAGCACAATGCGTTTAAAGACGGCAGTTAAAGCAAACAGCGTATCCAACGGGATCATCGCCGCTTCATCCACAAATAACCATAAATCCGCAAATTGCTGCGGATTTTCAc                                                                                         >  1:102530/1‑250 (MQ=255)                                                                                                               cgcTCCAGTTTGTCATCCGCCTGCCAACGTAGCGGGCTGAACAATTCAAAATGCCGAAAAGTGCGGTCGGTGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAATGGTCGTGGTCAGCACAATGCGTTTAAAGACGGCAGTTAAAGCAAACAGCGTATCCAACGGGATCATCGCCGCTTCATCCACAAATAACCATAAATCCGCAAATTGCTGCGGATTTTCACCaatc                                                                                    <  1:766206/249‑3 (MQ=255)                                                                                                                      gTTTGTCATCCGCCTGCCAACGTAGCGGGCTGAACAATTCAAAATGCCGAAAAGTGCGGTCGGTGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAATGGTCGTGGTCAGCACAATGCGTTTAAAGACGGCAGTTAAAGCAAACAGCGTATCCAACGGGATCATCGCCGCTTCATCCACAAATAACCATAAATCCGCAAATTGCTGCGGATTTTCACCAATCtttcgacg                                                                            >  1:688801/1‑245 (MQ=255)                                                                                                                       tttGTCATCCGCCTGCCAACGTAGCGGGCTGAACAATTCAAAATGCCGAAAAGTGCGGTCGGTGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAATGGTCGTGGTCAGCACAATGCGTTTAAAGACGGCAGTTAAAGCAAACAGCGTATCCAACGGGATCATCGCCGCTTCATCCACAAATAACCATAAATCCGCAAATTGCTGCGGATTTTCACCAATCtttcgacgc                                                                           <  1:230114/250‑7 (MQ=255)                                                                                                                          gTCATCCGCCTGCCAACGTAGCGGGCTGAACAATTCAAAATGCCGAAAAGTGCGGTCGGTGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAATGGTCGTGGTCAGCACAATGCGTTTAAAGACGGCAGTTAAAGCAAACAGCGTATCCAACGGGATCATCGCCGCTTCATCCACAAATAACCATAAATCCGCAAATTGCTGCGGATTTTCACCAATCTTTCGACGCAGtt                                                                       >  1:210795/1‑251 (MQ=255)                                                                                                                                    tGCCAACGTAGCGGGCTGAACAATTCAAAATGCCGAAAAGTGCGGTCGGTGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAATGGTCGTGGTCAGCACAATGCGTTTAAAGACGGCAGTTAAAGCAAACAGCGTATCCAACGGGatc                                                                                                                                                <  1:714248/168‑1 (MQ=255)                                                                                                                                    tGCCAACGTAGCGGGCTGAACAATTCAAAATGCCGAAAAGTGCGGTCGGTGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAATGGTCGTGGTCAGCACAATGCGTTTAAAGACGGCAGTTAAAGCAAACAGCGTATCCAACGGGATCATCGCCGCTTCATCCACAAATAACCATAAATCCGCAAATTGCTGCGGATTTTCACCAATCTTTCGACGCAGTTCATCAgg                                                                >  1:260459/1‑248 (MQ=255)                                                                                                                                        aaCGTAGCGGGCTGAACAATTCAAAATGCCGAAAAGTGCGGTCGGTGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAATGGTCGTGGTCAGCACAATGCGTGTAAAGACGGCAGTTAAAGCAAACAGCGTATCCAACGGGATCATCGCCGCTTCATCCACAAATAACCATAAATCCGCAAATTGCTGCGGATTTTCACCAATCTTTCGACGCAGTTCATCAggcgatata                                                         <  1:580939/251‑8 (MQ=255)                                                                                                                                          cGTAGCGGGCTGAACAATTCAAAATGCCGAAAAGTGCGGTCGGTGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAATGGTCGTGGTCAGCACAATGCGTTTAAAGACGGCAGTTAAAGCAAACAGCGTATCCAACGGGATCATCGCCGCTTCATCCACAAATAACCATAAATCCGCAAATTGCTGCGGATTTTCACCAATCTTTCGACGCAGTTCATCAggcgatataaa                                                       >  1:513996/1‑242 (MQ=255)                                                                                                                                          cGTAGCGGGCTGAACAATTCAAAATGCCGAAAAGTGCGGTCGGTGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAATGGTCGTGGTCAGCACAATGCGTTTAAAGACGGCAGTTAAAGCAAACAGCGTATCCAACGGGATCATCGCCGCTTCATCCACAAATAACCATAAATCCGCAAATTGCTGCGGATTTTCACCAATCTTTCGACGCAGTTCATCAggcgatataaa                                                       >  1:552992/1‑242 (MQ=255)                                                                                                                                                gggCTGAACAATTCAAAATGCCGAAAAGTGCGGTCGGTGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAATGGTCGTGGTCAGCACAATGCGTTTAAAGACGGCAGTTAAAGCAAACAGCGTATCCAACGGGATCATCGCCGCTTCATCCACAAATAAc                                                                                                                        <  1:266618/180‑1 (MQ=255)                                                                                                                                                gggCTGAACAATTCAAAATGCCGAAAAGTGCGGTCGGTGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAATGGTCGTGGTCAGCACAATGCGTTTAAAGACGGCAGTTAAAGCAAACAGCGTATCCAACGGGATCATCGCCGCTTCATCCACAAATAACCATAAATCCGCAAATTGCTGCGGATTTTCACCAATCTTTCGACGCAGTTCATCAggcgatataaacg                                                     >  1:192737/1‑236 (MQ=255)                                                                                                                                                 ggCTGAACAATTCAAAATGCCGAAAAGTGCGGTCGGTGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAATGGTCGTGGTCAGCACAATGCGTTTAAAGACGGCAGTTAAAGCAAACAGCGTATCCAACGGGATCATCGCCGCTTCATCCACAAATAAcc                                                                                                                       >  1:655524/1‑180 (MQ=255)                                                                                                                                                     ctattgacggcgctgaacaaaaGTGCGGTCGGTGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAATGGTCGTGGTCAGCACAATGCGTTTAAAGACGGCAGTTAAAGCAAACAGCGTATCCAACGGGATCATCGCCGCTTCATCCACAAATAACCATAAATCCGCAAATTGCTGCGGATTTTCACCAATCTTTCGACGCAGTTCATCAGGCGATATAAACGGGATTTccg                                            >  1:401924/20‑249 (MQ=255)                                                                                                                                                          aTTCAAAATGCCGAAAAGTGCGGTCGGTGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAATGGTCGTGGTCAGCACAATGCGTTTAAAGACGGCAGTTAAAGCAAACAGCGTATCCAACGGGATCATCGCCGCTTCATCCACAAATAACCATAAATCCGCAAAtt                                                                                                        >  1:819218/1‑186 (MQ=255)                                                                                                                                                          aTTCAAAATGCCGAAAAGTGCGGTCGGTGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAATGGTCGTGGTCAGCACAATGCGTTTAAAGACGGCAGTTAAAGCAAACAGCGTATCCAACGGGATCATCGCCGCTTCATCCACAAATAACCATAAATCCGCAAATTGCTGCGGATTTTCACCAATCTTTCGACGCAGt                                                                        >  1:267978/1‑218 (MQ=255)                                                                                                                                                          aTTCAAAATGCCGAAAAGTGCGGTCGGTGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAATGGTCGTGGTCAGCACAATGCGTTTAAAGACGGCAGTTAAAGCAAACAGCGTATCCAACGGGATCATCGCCGCTTCATCCACAAATAACCATAAATCCGCAAATTGCTGCGGATTTTCACCAATCTTTCGACGCAGTTCATCAgg                                                                >  1:440071/1‑226 (MQ=255)                                                                                                                                                                   gaacaaaaGTGCGGTCGGTGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAATGGTCGTGGTCAGCACAATGCGTTTAAAGACGGCAGTTAAAGCAAACAGCGTATCCAACGGGATCATCGCCGCTTCATCCACAAATAACCataaatc                                                                                                                >  1:180766/6‑163 (MQ=255)                                                                                                                                                                              cggtcggtGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAATGGTCGTGGTCAGCACAATGCGTTTAAAGACGGCAGTTAAAGCAAACAGCGTAGCCAACGGGATCATCGCCGCTTCATCCACAAATAACCATAAATCCGCAAATTGCTGCGGATTTTCACCAATCTTTCGACGCAGTTCATCAGGCGATATAACCGGGATTTCCGCAGCGGCAAATTCATTGAAAAtat                    <  1:879924/250‑3 (MQ=255)                                                                                                                                                                                gtcggtGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAATGGTCGTGGTCAGCACAATGCGTTTAAAGACGGCAGTTAAAGCAAACAGCGTATCCAACGGGATCATCGCCGCTTCATCCACAAATAACCATAAATCCGCAAATTGCTGCGGATTTTCACCAATCTTTCGACGCAGTTCATCAGGCGATATAAACGGGATTTCCGCAGCGGCAAATTCATTGAAAATATTg                  <  1:196735/250‑1 (MQ=255)                                                                                                                                                                                gtcggtGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAATGGTCGTGGTCAGCACAATGCGTTTAAAGACGGCAGTTAAAGCAAACAGCGTATCCAACGGGATCATCGCCGCTTCATCCACAAATAACCATAAATCCGCAAATTGCTGCGGATTTTCACCAATCTTTCGACGCAGTTCATCAGGCGATATAAACGGGATTTCCGCAGCGGCAAATTCATTGAAAATATTg                  <  1:777083/250‑1 (MQ=255)                                                                                                                                                                                gtcggtGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAATGGTCGTGGTCAGCACAATGCGTTTAAAGACGGCAGTTAAAGCAAACAGCGTATCCAACGGGATCATCGCCGCTTCATCCACAAATAACCATAAATCCGCAAATTGCTGCGGATTTTCACCAATCTTTCGACGCAGTTCATCAGGCGATATAAACGGGATTTCCGCAGCGGCAAATTCATTGAAAATATTg                  <  1:408353/250‑1 (MQ=255)                                                                                                                                                                                gtcggtGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTAACCTCGTAACTGTGAATGGTCGTGGTCAGCACAATGCGTTTAAAGACGGCAGTTAAAGCAAACAGCGTATCCAACGGGATCATCGCCGCTTCATCCACAAATAACCATAAATCCGCAAATTGCTGCGGATTTTCACCAATCTTTCGACGCAGTTCATCAGGCGATATAAACGGGATTTCCGCAGCGGCAAATTCATTGAAAATATTg                  <  1:872472/250‑1 (MQ=255)                                                                                                                                                                                 tcggtgttcgctaTGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAATGGTCGTGGTCAGCACAATGCGTTTAAAGACGGCAGTTAAAGCAAACAGCGTATCCAACGGGATCATCGCCGCTTCATCCACAAATAACCATAAATCCGCAAATTGCTGCGGATTTTCACCAATCTTTCGACGCAGTTCATCAGGCGATATAAACGGGATTTCCGCAGCGGCAAATTCATTGAAAATATTGAc                <  1:265432/239‑1 (MQ=255)                                                                                                                                                                                    gtgttcgctaTGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAATGGTCGTGGTCAGCACAATGCGTTTAAAGACGGCAGTTAAAGCAAACAGCGTATCCAACGGGATCATCGCCGCTTCATCCACAAATAACCat                                                                                                                     <  1:669801/138‑2 (MQ=255)                                                                                                                                                                                    gtgttcgctaTGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAATGGTCGTGGTCAGCACAATGCGTTTAAAGACGGCAGTTAAAGCAAACAGCGTATCCAACGGGATCATCGCCGCTTCATCCACAAATAACCATAAATCCGCAAATTGCTGCGGATTTTCACCAATCTTTCGACGCAGTTCATCAGGCGATATAAACGGGATTTCCGCAGCGGCAAATTCATTGAAAATATTGACCg              >  1:803534/10‑250 (MQ=255)                                                                                                                                                                                    gtgttcgctaTGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAATGGTCGTGGTCAGCACAATGCGTTTAAAGACGGCAGTTAAAGCAAACAGCGTATCCAACGGGATCATCGCCGCTTCATCCACAAATAACCATAAATCCGCAAATTGCTGCGGATTTTCACCAATCTTTCGACGCAGTTCATCAGGCGATATAAACGGGATTTCCGCAGCGGCAAATTCATTGAAAATATTGACCg              >  1:348820/10‑250 (MQ=255)                                                                                                                                                                                              tGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAATGGTCGTGGTCAGCACAATGCGTTTAAAGACGGCAGTTAAAGCAAACAGCGTATCCAACGGGATCATCGCCGCTTCATCCACAAATAACCATAAATCCGCAAATTGCTGCGGATTTTCACCAATCTTTCGACGCAGTTCATCAGGCGATATAAACGGGATTTCCGCAGCGGCAAATTCATTGAAAATATTGACCGCACTTTTGTTc   <  1:856567/251‑1 (MQ=255)                                                                                                                                                                                               gAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAATGGTCGTGGTCAGCACAATGCGTTTAAAGACGGCAGTTAAAGCAAACAGCGTATCCAACGGGATCATCGCCGCTTCATCCACAAATAACCATAAATCCGCAAATTGCTGCGGATTTTCACCAATCTTTCGACGCAGTTCATCAGGCGATATAAACGGGATTTCCGCAGCGGCAAATTCATTGAAAATATTGACCGCACTTTTGTTca  >  1:234747/1‑250 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                        GCGGAATCTGTTCTTCGTAAGAAATCTGAATCTGTTTGGCAAGGTTGGCATCGTAACGCGGTTGCGTCAGACGATCTTCGCAATCACACAACAACAATTCATCAATAAAGCGCTCCAGTTTGTCATCCGCCTGCCAACGTAACGGGCTGAACAATTCAAAATGCCGCAAAGTGCGGTCGGTTTTCGTCATGAATTTTAACAAGAAGCCCCTGCCCGTGCCTTCGTAGCTGTGAATGGTGGTCGTCAGCACAATACGTTTAAAGGCAGTCGTTAAATCAAACAGCGTATCCAACGGAATCATCGCCGCTTCGTCCACAAATAACCACGAATCCGCAAATTGCTGTGGATTTTCACCAACCTTTTGGCGCAGTTCATCAGGTGGCATAAAGGGGATTTCGACACCGGAAAATTCATTGAAAATCTTGACCGCACTTTTGTTCG  >  1/1475012‑1475452

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑