Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,475,238	G→A	S280S (AGC→AGT)	unknown ←	tRNA cytosine(34) acetyltransferase (EC 2.3.1.193)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,475,238	0	G	A	100.0%	177.6 / NA	66	S280S (AGC→AGT)	unknown	tRNA cytosine(34) acetyltransferase (EC 2.3.1.193)
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (31/35);  total (31/35)

AAACCGATTCGATTTGATAAGGCGGAATCTGTTCTTCGTAAGAAATCTGAATCTGTTTGGCAAGGTTGGCATCGTAACGCGGTTGCGTCAGACGATCTTCGCAATCACACAACAACAATTCATCAATAAAGCGCTCCAGTTTGTCATCCGCCTGCCAACGTAACGGGCTGAACAATTCAAAATGCCGCAAAGTGCGGTCGGTTTTCGTCATGAATTTTAACAAGAAGCCCCTGCCCGTGCCTTCGTAGCTGTGAATGGTGGTCGTCAGCACAATACGTTTAAAGGCAGTCGTTAAATCAAACAGCGTATCCAACGGAATCATCGCCGCTTCGTCCACAAATAACCACGAATCCGCAAATTGCTGTGGATTTTCACCAACCTTTTGGCGCAGTTCATCAGGTGGCATAAAGGGGATTTCGACACCGGAAAATTCATTGAAAATCTTGACCGCACTTTTGTTCG  >  1/1474991‑1475452                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                       aaaCCGATTCGATTTGATTAGGCGGAATCCGTTCTTTGTAAGAAATCTGAATCTGTTCGGCAAGGTTAGCGTCATAACACGGTTGCGGCAGACGATCTTCGCAATCACACAACAACAATTCGTCAATAAAGCGCTCCAGTTTGTCATCCGCCTGCCAACGTAGCGGGCTGAACAATTCAAAATGCCGAAAAGTGCGGTCGGTGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACtg                                                                                                                                                                                                                     <  1:807094/251‑1 (MQ=255)         tcgattTGATTAGGCGGAATCCGTTCTTTGTAAGAAATCTGAATCTGTTCGGCAAGGTTAGCGTCATAACACGGTTGCGGCAGACGATCTTCGCAATCACACAACAACAATTCGTCAATAAAGCGCTCCAGTTTGTCATCCGCCTGCCAACGTAGCGGGCTGAACAATTCAAAATGCCGAAAAGTGCGGTCGGTGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAAtggt                                                                                                                                                                                                             <  1:103846/251‑1 (MQ=255)                gattaGGCGGAATCCGTTCTTTGTAAGAAATCTGAATCTGTTCGGCAAGGTTAGCGTCATAACACGGTTGCGGCAGACGATCTTCGCAATCACACAACAACAATTCGTCAATAAAGCGCTCCAGTTTGTCATCCGCCTGCCAACGTAGCGGGCTGAACAATTCAAAATGCCGAAAAGTGCGGTCGGTGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAAtggtcgtggt                                                                                                                                                                                                       >  1:499629/5‑244 (MQ=255)                      gCGGAATCCGTTCTTTGTAAGAAATCTGAATCTGTTCGGCAAGGTTAGCGTCATAACACGGTTGCGGCAGACGATCTTCGCAATCACACAACAACAATTCGTCAATAAAGCGCTCCAGTTTGTCATCCGCCTGCCAACGTAGCGGGCTGAACAATTCAAAATGCCGAAAAGTGCGGTCGGTGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAATGGTCGTGGTCAGcac                                                                                                                                                                                                 <  1:109686/250‑1 (MQ=255)                      gCGGAATCCGTTCTTTGTAAGAAATCTGAATCTGTTCGGCAAGGTTAGCGTCATAACACGGTTGCGGCAGACGATCTTCGCAATCACACAACAACAATTCGTCAATAAAGCGCTCCAGTTTGTCATCCGCCTGCCAACGTAGCGGGCTGAACAATTCAAAATGCCGAAAAGTGCGGTCGGTGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAATGGTCGTGGTCAGcac                                                                                                                                                                                                 <  1:408326/250‑1 (MQ=255)                      gCGGAATCCGTTCTTTGTAAGAAATCTGAATCTGTTCGGCAAGGTTAGCGTCATAACACGGTTGCGGCAGACGATCTTCGCAATCACACAACAACAATTCGTCAATAAAGCGCTCCAGTTTGTCATCCGCCTGCCAACGTAGCGGGCTGAACAATTCAAAATGCCGAAAAGTGCGGTCGGTGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAATGGTCGTGGTCAGcac                                                                                                                                                                                                 <  1:328430/250‑1 (MQ=255)                      gCGGAATCCGTTCTTTGTAAGAAATCTGAATCTGTTCGGCAAGGTTAGCGTCATAACACGGTTGCGGCAGACGATCTTCGCAATCACACAACAACAATTCGTCAATAAAGCGCTCCAGTTTGTCATCCGCCTGCCAACGTAGCGGGCTGAACAATTCAAAATGCCGAAAAGTGCGGTCGGTGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAATGGTCGTGGTCAGcac                                                                                                                                                                                                 <  1:713065/250‑1 (MQ=255)                         gAATCCGTTCTTTGTAAGAAATCTGAATCTGTTCGGCAAGGTTAGCGTCATAACACGGTTGCGGCAGACGATCTTCGCAATCACACAACAACAATTCGTCAATAAAGCGCTCCAGTTTGTCATCCGCCTGCCAACGTAGCGGGCTGAACAATTCAAAATGCCGAAAAGTGCGGTCGGTGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAATGGTCGTGGTCAGCACaa                                                                                                                                                                                               >  1:535326/1‑249 (MQ=255)                         gAATCCGTTCTTTGTAAGAAATCTGAATCTGTTCGGCAAGGTTAGCGTCATAACACGGTTGCGGCAGACGATCTTCGCAATCACACAACAACAATTCGTCAATAAAGCGCTCCAGTTTGTCATCCGCCTGCCAACGTAGCGGGCTGAACAATTCAAAATGCCGAAAAGTACGGTCGGTGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAATGGTCGTGGTCAGCACaa                                                                                                                                                                                               >  1:447481/1‑249 (MQ=255)                          aaTCCGTTCTTTGTAAGAAATCTGAATCTGTTCGGCAAGGTTAGCGTCATAACACGGTTGCGGCAGACGATCTTCGCAATCACACAACAACAATTCGTCAATAAAGCGCTCCAGTTTGTCATCCGCCTGCCAACGTAGCGGGCTGAACAATTCAAAATGCCGAAAAGTGCGGTCGGTGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAATGGTCGTGGTCAGCACAAtgc                                                                                                                                                                                            <  1:429962/251‑3 (MQ=255)                                  ctttgTAAGAAATCTGAATCTGTTCGGCAAGGTTAGCGTCATAACACGGTTGCGGCAGACGATCTTCGCAATCACACAACAACAATTCGTCAATAAAGCGCTCCAGTTTGTCATCCGCCTGCCAACGTAGCGGGCTGAACAATTCAAAATGCCGAAAAGTGCGGTCGGTGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAATGGTCGTGGTCAGCACAATGCGTTTAAAg                                                                                                                                                                                    <  1:801365/247‑1 (MQ=255)                                                        ttcggCAAGGTTAGCGTCATAACACGGTTGCGGCAGACGATCTTCGCAATCACACAACAACAATTCGTCAATAAAGCGCTCCAGTTTGTCATCCGCCTGCCAACGTAGCGGGCTGAACAATTCAAAATGCCGAAAAGTGCGGTCGGTGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAATGGTCGTGGTCAGCACAATGCGTTTAAAGACGGCAGTTAAAGCAAACAGCg                                                                                                                                                              <  1:595812/248‑1 (MQ=255)                                                        ttcggCAAGGTTAGCGTCATAACACGGTTGCGGCAGACGATCTTCGCAATCACACAACAACAATTCGTCAATAAAGCGCTCCAGTTTGTCATCCGCCTGCCAACGTAGCGGGCTGAACAATTCAAAATGCCGAAAAGTGCGGTCGGTGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAATGGTCGTGGTCAGCACAATGCGTTTAAAGACGGCAGTTAAAGCAAACAGCg                                                                                                                                                              <  1:279059/248‑1 (MQ=255)                                                            gCAAGGTTAGCGTCATAACACGGTTGCGGCAGACGATCTTCGCAATCACACAACAACAATTCGTCAATAAAGCGCTCCAGTTTGTCATCCGCCTGCCAACGTAGCGGGCTGAACAATTCAAAATGCCGAAAAGTGCGGTCGGTGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAATGGTCGTGGTCAGCACAATGCGTTTAAAGACGGCAGTTAAAGCAAACAGCGTAt                                                                                                                                                           <  1:462804/250‑1 (MQ=255)                                                                ggttagcgtcatAACACGGTTGCGGCAGACGATCTTCGCAATCACACAACAACAATTCGTCAATAAAGCGCTCCAGTTTGTCATCCGCCTGCCAACGTAGCGGGCTGAACAATTCAAAATGCCGAAAAGTGCGGTCGGTGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAATGGTCGTGGTCAGCACAATGCGTTTAAAGACGGCAGTTAAAGCAAACAGCGTATCCaa                                                                                                                                                       >  1:386367/12‑250 (MQ=255)                                                                 gttagcgtcatAACACGGTTGCGGCAGACGATCTTCGCAATCACACAACAACAATTCGTCAATAAAGCGCTCCAGTTTGTCATCCGCCTGCCAACGTAGCGGGCTGAACAATTCAAAATGCCGAAAAGTGCGGTCGGTGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAATGGTCGTGGTCAGCACAATGCGTTTAAAGACGGCAGTTAAAGCAAACAGCGTATCCAAc                                                                                                                                                      <  1:574376/240‑1 (MQ=255)                                                                     gcgtcatAACACGGTTGCGGCAGACGATCTTCGCAATCACACAACAACAATTCGTCAATAAAGCGCTCCAGTTTGTCATCCGCCTGCCAACGTAGCGGGCTGAACAATTCAAAATGCCGAAAAGTGCGGTCGGTGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGa                                                                                                                                                                                                                  >  1:526238/7‑186 (MQ=255)                                                                      cgtcatAACACGGTTGCGGCAGACGATCTTCGCAATCACACAACAACAATTCGTCAATAAAGCGCTCCAGTTTGTCATCCGCCTGCCAACGTAGCGGGCTGAACAATTCAAAATGCCGAAAAGTGCGGTCGGTGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAATGGTCGTGGTCAGCACAATGCGTTTAAAGACGGCAGTTAAAGCAAACAGCGTATCCAACgggc                                                                                                                                                  <  1:538881/244‑3 (MQ=255)                                                                      cgtcatAACACGGTTGCGGCAGACGATCTTCGCAATCACACAACAACAATTCGTCAATAAAGCGCTCCAGTTTGTCATCCGCCTGCCAACGTAGCGGGCTGAACAATTCAAAATGCCGAAAAGTGCGGTCGGTGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAATGGTCGTGGTCAGCACAATGCGTTTAAAGACGGCAGTTAAAGCAAACAGCGTATCCAACgggat                                                                                                                                                 <  1:481754/245‑4 (MQ=255)                                                                        tcatAACACGGTTGCGGCAGACGATCTTCGCAATCACACAACAACAATTCGTCAATAAAGCGCTCCAGTTTGTCATCCGCCTGCCAACGTAGCGGGCTGAACAATTCAAAATGCCGAAAAGTGCGGTCGGTGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAATGGTCGTGGTCAGCACAATGCGTTTAAAGACGGCAGTTAAAGCAAACAGCGTATc                                                                                                                                                          >  1:17517/4‑239 (MQ=255)                                                                        tcatAACACGGTTGCGGCAGACGATCTTCGCAATCACACAACAACAATTCGTCAATAAAGCGCTCCAGTTTGTCATCCGCCTGCCAACGTAGCGGGCTGAACAATTCAAAATGCCGAAAAGTGCGGTCGGTGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAATGGTCGTGGTCAGCACAATGCGTTTAAAGACGGCAGTTAAAGCAAACAGCGTATCCAACgggat                                                                                                                                                 <  1:227623/245‑4 (MQ=255)                                                                        tcatAACACGGTTGCGGCAGACGATCTTCGCAATCACACAACAACAATTCGTCAATAAAGCGCTCCAGTTTGTCATCCGCCTGCCAACGTAGCGGGCTGAACAATTCAAAATGCCGAAAAGTGCGGTCGGTGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAATGGTCGTGGTCAGCACAATGCGTTTAAAGACGGCAGTTAAAGCAAACAGCGTATCCAACGGGatcat                                                                                                                                              >  1:144935/4‑251 (MQ=255)                                                                           tAACACGGTTGCGGCAGACGATCTTCGCAATCACACAACAACAATTCGTCAATAAAGCGCTCCAGTTTGTCATCCGCCTGCCAACGTAGCGGGCTGAACAATTCAAAATGCCGAAAAGTGCGGTCGGTGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAATGGTCGTGGTCAGcac                                                                                                                                                                                                 <  1:887053/197‑1 (MQ=255)                                                                           tAACACGGTTGCGGCAGACGATCTTCGCAATCACACAACAACAATTCGTCAATAAAGCGCTCCAGTTTGTCATCCGCCTGCCAACGTAGCGGGCTGAACAATTCAAAATGCCGAAAAGTGCGGTCGGTGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAATGGTCGTGGTCAGCACAATGCGTTTAAAgacggc                                                                                                                                                                               <  1:840581/215‑6 (MQ=255)                                                                                 ggTTGCGGCAGACGATCTTCGCAATCACACAACAACAATTCGTCAATAAAGCGCTCCAGTTTGTCATCCGCCTGCCAACGTAGCGGGCTGATCAATTCAAAATGCCGAAAAGTGCGGTCGGTGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAATGGTCGTGGTCAGCACAATGCGTTTAAAGACGGCAGTTAAAGCAAACAGCGTATCCAACGGGATCATCGCCGCtt                                                                                                                                      >  1:617817/1‑250 (MQ=255)                                                                                    tGCGGCAGACGATCTTCGCAATCACACAACAACAATTCGTCAATAAAGCGCTCCAGTTTGTCATCCGCCTGCCAACGTAGCGGGCTGAACAATTCAAAATGCCGAAAAGTGCGGTCGGTGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAATGGTCGTGGTCAGCACAATGCGTTTAAAGACGGCAGTTAAAGCAAACAGCGTATCCAACGGGATCATCGCCGCTTcatc                                                                                                                                  <  1:699861/251‑4 (MQ=255)                                                                                               aTCTTCGCAATCACACAACAACAATTCGTCAATAAAGCGCTCCAGTTTGTCATCCGCCTGCCAACGTAGCGGGCTGAACAATTCAAAATGCCGAAAAGTGCGGTCGGTGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAAtggtcgtggtc                                                                                                                                                                                                      >  1:673172/1‑165 (MQ=255)                                                                                               aTCTTCGCAATCACACAACAACAATTCGTCAATAAAGCGCTCCAGTTTGTCATCCGCCTGCCAACGTAGCGGGCTGAACAATTCAAAATGCCGAAAAGTGCGGTCGGTGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAATGGTCGTGGTCAGCACAATGCGTTTAAAGACGGCAGTTAAAGCAAACAGCGTATCCAACGGGATCATCGCCGCTTCATCCACAAATAAcc                                                                                                                       <  1:523360/251‑1 (MQ=255)                                                                                                            cacaACAACAATTCGTCAATAAAGCGCTCCAGTTTGTCATCCGCCTGCCAACGTAGCGGGCTGAACAATTCAAAATGCCGAAAAGTGCGGTCGGTGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAATGGTCGTGGTCAGCACAATGCGTTTAAAGACGGCAGTTAAAGCAAACAGCGTATCCAACGGGATCATcgc                                                                                                                                           >  1:218698/1‑218 (MQ=255)                                                                                                              caacaacaaTTCGTCAATAAAGCGCTCCAGTTTGTCATCCGCCTGCCAACGTAGCGGGCTGAACAATTCAAAATGCCGAAAAGTGCGGTCGGTGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAATGGTCGTGGTCAGCACAATGCGTTTAAAGACGGCAGTTAAAGCAAACAGCGTATCCAACggga                                                                                                                                                  <  1:778456/209‑3 (MQ=255)                                                                                                              caacaacaaTTCGTCAATAAAGCGCTCCAGTTTGTCATCCGCCTGCCAACGTAGCGGGCTGAACAATTCAAAATGCCGAAAAGTGCGGTCGGTGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAATGGTCGTGGTCAGCACAATGCGTTTAAAGACGGCAGTTAAAGCAAACAGCGTATCCAACGGGATCATCGCCGCTTCATCCACAAATAACCATAAATCCGCAAAtt                                                                                                        >  1:772507/1‑251 (MQ=255)                                                                                                                acaacaaTTCGTCAATAAAGCGCTCCAGTTTGTCATCCGCCTGCCAACGTAGCGGGCTGAACAATTCAAAATGCCGAAAAGTGCGGTCGGTGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAATGGTCGTGGTCAGCACAATGCGTTTAAAGACGGCAGTTAAAGCAAACAGCGTATCCAACgg                                                                                                                                                    <  1:207128/205‑1 (MQ=255)                                                                                                                      aTTCGTCAATAAAGCGCTCCAGTTTGTCATCCGCCTGCCAACGTAGCGGGCTGAACAATTCAAAATGCCGAAAAGTGCGGTCGGTGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAATGGTCGTGGTCAGCACAATGCGTTTAAAGACGGCAGTTAAAGCAAACAGCGTATCCAACGGGATCATCGCCGCTTCATCCACAAATAACCataaatc                                                                                                                >  1:6438/1‑229 (MQ=255)                                                                                                                          gtcaATAAAGCGCTCCAGTTTGTCATCCGCCTGCCAACGTAGCGGGCTGAACAATTCAAAATGCCGAAAAGTGCGGTCGGTGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAATGGTCGTGGTCAGCACAATGCGTTTAAAGACGGCAGTTAAAGCAAACAGCGTATccc                                                                                                                                                        >  1:592032/2‑190 (MQ=255)                                                                                                                          gtcaATAAAGCGCTCCAGTTTGTCATCCGCCTGCCAACGTAGCGGGCTGAACAATTCAAAATGCCGAAAAGTGCGGTCGGTGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAATGGTCGTGGTCAGCACAATGCGTTTAAAGACGGCAGTTAAAGCAAACAGCGTATCCAACGGGATCATCGCCGCTTCATCCACAAATAACCATAAATCCGCAAATTGCTGCGGAtttt                                                                                            >  1:86345/2‑251 (MQ=255)                                                                                                                           tcaATAAAGCGCTCCAGTTTGTCATCCGCCTGCCAACGTAGCGGGCTGAACAATTCAAAATGCCGAAAAGTGCGGTCGGTGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAATGGTCGTGGTCAGCACAATGCGTTTAAAGACGGCAGTTAAAGCAAACAGCGTATCCAACGGGATCATCGCCGCTTCATCCACAAATAACCATAAATCCGCAAATTGCTGCGGAtttt                                                                                            >  1:443041/1‑250 (MQ=255)                                                                                                                              aTAAAGCGCTCCAGTTTGTCATCCGCCTGCCAACGTAGCGGGCTGAACAATTCAAAATGCCGAAAAGTGCGGTCGGTGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAATGGTCGTGGTCAGCACAATGCGTTTAAAGACGGCAGTTAAAGCAAACAGCGTATCCAACGGGATCATCGCCGCTTCATCCACAAATAACCATAAATCCGCAAATTGCTGCGGATTTTCAc                                                                                         >  1:102530/1‑250 (MQ=255)                                                                                                                                    cgcTCCAGTTTGTCATCCGCCTGCCAACGTAGCGGGCTGAACAATTCAAAATGCCGAAAAGTGCGGTCGGTGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAATGGTCGTGGTCAGCACAATGCGTTTAAAGACGGCAGTTAAAGCAAACAGCGTATCCAACGGGATCATCGCCGCTTCATCCACAAATAACCATAAATCCGCAAATTGCTGCGGATTTTCACCaatc                                                                                    <  1:766206/249‑3 (MQ=255)                                                                                                                                      cTCCAGTTTGTCATCCGCCTGCCAACGTAGCGGGCTGAACAATTCAAAATGCCGAAAAGTGCGGTCGGTGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAAtggt                                                                                                                                                                                                             <  1:204601/126‑1 (MQ=255)                                                                                                                                           gTTTGTCATCCGCCTGCCAACGTAGCGGGCTGAACAATTCAAAATGCCGAAAAGTGCGGTCGGTGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAATGGTCGTGGTCAGCACAATGCGTTTAAAGACGGCAGTTAAAGCAAACAGCGTATCCAACGGGATCATCGCCGCTTCATCCACAAATAACCATAAATCCGCAAATTGCTGCGGATTTTCACCAATCtttcgacg                                                                            >  1:688801/1‑245 (MQ=255)                                                                                                                                            tttGTCATCCGCCTGCCAACGTAGCGGGCTGAACAATTCAAAATGCCGAAAAGTGCGGTCGGTGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAATGGTCGTGGTCAGCACAATGCGTTTAAAGACGGCAGTTAAAGCAAACAGCGTATCCAACGGGATCATCGCCGCTTCATCCACAAATAACCATAAATCCGCAAATTGCTGCGGATTTTCACCAATCtttcgacgc                                                                           <  1:230114/250‑7 (MQ=255)                                                                                                                                               gTCATCCGCCTGCCAACGTAGCGGGCTGAACAATTCAAAATGCCGAAAAGTGCGGTCGGTGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAATGGTCGTGGTCAGCACAATGCGTTTAAAGACGGCAGTTAAAGCAAACAGCGTATCCAACGGGATCATCGCCGCTTCATCCACAAATAACCATAAATCCGCAAATTGCTGCGGATTTTCACCAATCTTTCGACGCAGtt                                                                       >  1:210795/1‑251 (MQ=255)                                                                                                                                                         tGCCAACGTAGCGGGCTGAACAATTCAAAATGCCGAAAAGTGCGGTCGGTGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAATGGTCGTGGTCAGCACAATGCGTTTAAAGACGGCAGTTAAAGCAAACAGCGTATCCAACGGGatc                                                                                                                                                <  1:714248/168‑1 (MQ=255)                                                                                                                                                         tGCCAACGTAGCGGGCTGAACAATTCAAAATGCCGAAAAGTGCGGTCGGTGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAATGGTCGTGGTCAGCACAATGCGTTTAAAGACGGCAGTTAAAGCAAACAGCGTATCCAACGGGATCATCGCCGCTTCATCCACAAATAACCATAAATCCGCAAATTGCTGCGGATTTTCACCAATCTTTCGACGCAGTTCATCAgg                                                                >  1:260459/1‑248 (MQ=255)                                                                                                                                                             aaCGTAGCGGGCTGAACAATTCAAAATGCCGAAAAGTGCGGTCGGTGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAATGGTCGTGGTCAGCACAATGCGTGTAAAGACGGCAGTTAAAGCAAACAGCGTATCCAACGGGATCATCGCCGCTTCATCCACAAATAACCATAAATCCGCAAATTGCTGCGGATTTTCACCAATCTTTCGACGCAGTTCATCAggcgatata                                                         <  1:580939/251‑8 (MQ=255)                                                                                                                                                               cGTAGCGGGCTGAACAATTCAAAATGCCGAAAAGTGCGGTCGGTGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAATGGTCGTGGTCAGCACAATGCGTTTAAAGACGGCAGTTAAAGCAAACAGCGTATCCAACGGGATCATCGCCGCTTCATCCACAAATAACCATAAATCCGCAAATTGCTGCGGATTTTCACCAATCTTTCGACGCAGTTCATCAggcgatataaa                                                       >  1:552992/1‑242 (MQ=255)                                                                                                                                                               cGTAGCGGGCTGAACAATTCAAAATGCCGAAAAGTGCGGTCGGTGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAATGGTCGTGGTCAGCACAATGCGTTTAAAGACGGCAGTTAAAGCAAACAGCGTATCCAACGGGATCATCGCCGCTTCATCCACAAATAACCATAAATCCGCAAATTGCTGCGGATTTTCACCAATCTTTCGACGCAGTTCATCAggcgatataaa                                                       >  1:513996/1‑242 (MQ=255)                                                                                                                                                                     gggCTGAACAATTCAAAATGCCGAAAAGTGCGGTCGGTGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAATGGTCGTGGTCAGCACAATGCGTTTAAAGACGGCAGTTAAAGCAAACAGCGTATCCAACGGGATCATCGCCGCTTCATCCACAAATAAc                                                                                                                        <  1:266618/180‑1 (MQ=255)                                                                                                                                                                     gggCTGAACAATTCAAAATGCCGAAAAGTGCGGTCGGTGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAATGGTCGTGGTCAGCACAATGCGTTTAAAGACGGCAGTTAAAGCAAACAGCGTATCCAACGGGATCATCGCCGCTTCATCCACAAATAACCATAAATCCGCAAATTGCTGCGGATTTTCACCAATCTTTCGACGCAGTTCATCAggcgatataaacg                                                     >  1:192737/1‑236 (MQ=255)                                                                                                                                                                      ggCTGAACAATTCAAAATGCCGAAAAGTGCGGTCGGTGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAATGGTCGTGGTCAGCACAATGCGTTTAAAGACGGCAGTTAAAGCAAACAGCGTATCCAACGGGATCATCGCCGCTTCATCCACAAATAAcc                                                                                                                       >  1:655524/1‑180 (MQ=255)                                                                                                                                                                          ctattgacggcgctgaacaaaaGTGCGGTCGGTGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAATGGTCGTGGTCAGCACAATGCGTTTAAAGACGGCAGTTAAAGCAAACAGCGTATCCAACGGGATCATCGCCGCTTCATCCACAAATAACCATAAATCCGCAAATTGCTGCGGATTTTCACCAATCTTTCGACGCAGTTCATCAGGCGATATAAACGGGATTTccg                                            >  1:401924/20‑249 (MQ=255)                                                                                                                                                                               aTTCAAAATGCCGAAAAGTGCGGTCGGTGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAATGGTCGTGGTCAGCACAATGCGTTTAAAGACGGCAGTTAAAGCAAACAGCGTATCCAACGGGATCATCGCCGCTTCATCCACAAATAACCATAAATCCGCAAAtt                                                                                                        >  1:819218/1‑186 (MQ=255)                                                                                                                                                                               aTTCAAAATGCCGAAAAGTGCGGTCGGTGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAATGGTCGTGGTCAGCACAATGCGTTTAAAGACGGCAGTTAAAGCAAACAGCGTATCCAACGGGATCATCGCCGCTTCATCCACAAATAACCATAAATCCGCAAATTGCTGCGGATTTTCACCAATCTTTCGACGCAGt                                                                        >  1:267978/1‑218 (MQ=255)                                                                                                                                                                               aTTCAAAATGCCGAAAAGTGCGGTCGGTGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAATGGTCGTGGTCAGCACAATGCGTTTAAAGACGGCAGTTAAAGCAAACAGCGTATCCAACGGGATCATCGCCGCTTCATCCACAAATAACCATAAATCCGCAAATTGCTGCGGATTTTCACCAATCTTTCGACGCAGTTCATCAgg                                                                >  1:440071/1‑226 (MQ=255)                                                                                                                                                                                        gaacaaaaGTGCGGTCGGTGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAATGGTCGTGGTCAGCACAATGCGTTTAAAGACGGCAGTTAAAGCAAACAGCGTATCCAACGGGATCATCGCCGCTTCATCCACAAATAACCataaatc                                                                                                                >  1:180766/6‑163 (MQ=255)                                                                                                                                                                                                   cggtcggtGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAATGGTCGTGGTCAGCACAATGCGTTTAAAGACGGCAGTTAAAGCAAACAGCGTAGCCAACGGGATCATCGCCGCTTCATCCACAAATAACCATAAATCCGCAAATTGCTGCGGATTTTCACCAATCTTTCGACGCAGTTCATCAGGCGATATAACCGGGATTTCCGCAGCGGCAAATTCATTGAAAAtat                    <  1:879924/250‑3 (MQ=255)                                                                                                                                                                                                     gtcggtGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAATGGTCGTGGTCAGCACAATGCGTTTAAAGACGGCAGTTAAAGCAAACAGCGTATCCAACGGGATCATCGCCGCTTCATCCACAAATAACCATAAATCCGCAAATTGCTGCGGATTTTCACCAATCTTTCGACGCAGTTCATCAGGCGATATAAACGGGATTTCCGCAGCGGCAAATTCATTGAAAATATTg                  <  1:196735/250‑1 (MQ=255)                                                                                                                                                                                                     gtcggtGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAATGGTCGTGGTCAGCACAATGCGTTTAAAGACGGCAGTTAAAGCAAACAGCGTATCCAACGGGATCATCGCCGCTTCATCCACAAATAACCATAAATCCGCAAATTGCTGCGGATTTTCACCAATCTTTCGACGCAGTTCATCAGGCGATATAAACGGGATTTCCGCAGCGGCAAATTCATTGAAAATATTg                  <  1:777083/250‑1 (MQ=255)                                                                                                                                                                                                     gtcggtGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAATGGTCGTGGTCAGCACAATGCGTTTAAAGACGGCAGTTAAAGCAAACAGCGTATCCAACGGGATCATCGCCGCTTCATCCACAAATAACCATAAATCCGCAAATTGCTGCGGATTTTCACCAATCTTTCGACGCAGTTCATCAGGCGATATAAACGGGATTTCCGCAGCGGCAAATTCATTGAAAATATTg                  <  1:408353/250‑1 (MQ=255)                                                                                                                                                                                                     gtcggtGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTAACCTCGTAACTGTGAATGGTCGTGGTCAGCACAATGCGTTTAAAGACGGCAGTTAAAGCAAACAGCGTATCCAACGGGATCATCGCCGCTTCATCCACAAATAACCATAAATCCGCAAATTGCTGCGGATTTTCACCAATCTTTCGACGCAGTTCATCAGGCGATATAAACGGGATTTCCGCAGCGGCAAATTCATTGAAAATATTg                  <  1:872472/250‑1 (MQ=255)                                                                                                                                                                                                      tcggtgttcgctaTGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAATGGTCGTGGTCAGCACAATGCGTTTAAAGACGGCAGTTAAAGCAAACAGCGTATCCAACGGGATCATCGCCGCTTCATCCACAAATAACCATAAATCCGCAAATTGCTGCGGATTTTCACCAATCTTTCGACGCAGTTCATCAGGCGATATAAACGGGATTTCCGCAGCGGCAAATTCATTGAAAATATTGAc                <  1:265432/239‑1 (MQ=255)                                                                                                                                                                                                         gtgttcgctaTGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAATGGTCGTGGTCAGCACAATGCGTTTAAAGACGGCAGTTAAAGCAAACAGCGTATCCAACGGGATCATCGCCGCTTCATCCACAAATAACCat                                                                                                                     <  1:669801/138‑2 (MQ=255)                                                                                                                                                                                                         gtgttcgctaTGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAATGGTCGTGGTCAGCACAATGCGTTTAAAGACGGCAGTTAAAGCAAACAGCGTATCCAACGGGATCATCGCCGCTTCATCCACAAATAACCATAAATCCGCAAATTGCTGCGGATTTTCACCAATCTTTCGACGCAGTTCATCAGGCGATATAAACGGGATTTCCGCAGCGGCAAATTCATTGAAAATATTGACCg              >  1:803534/10‑250 (MQ=255)                                                                                                                                                                                                         gtgttcgctaTGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAATGGTCGTGGTCAGCACAATGCGTTTAAAGACGGCAGTTAAAGCAAACAGCGTATCCAACGGGATCATCGCCGCTTCATCCACAAATAACCATAAATCCGCAAATTGCTGCGGATTTTCACCAATCTTTCGACGCAGTTCATCAGGCGATATAAACGGGATTTCCGCAGCGGCAAATTCATTGAAAATATTGACCg              >  1:348820/10‑250 (MQ=255)                                                                                                                                                                                                                   tGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAATGGTCGTGGTCAGCACAATGCGTTTAAAGACGGCAGTTAAAGCAAACAGCGTATCCAACGGGATCATCGCCGCTTCATCCACAAATAACCATAAATCCGCAAATTGCTGCGGATTTTCACCAATCTTTCGACGCAGTTCATCAGGCGATATAAACGGGATTTCCGCAGCGGCAAATTCATTGAAAATATTGACCGCACTTTTGTTc   <  1:856567/251‑1 (MQ=255)                                                                                                                                                                                                                    gAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAATGGTCGTGGTCAGCACAATGCGTTTAAAGACGGCAGTTAAAGCAAACAGCGTATCCAACGGGATCATCGCCGCTTCATCCACAAATAACCATAAATCCGCAAATTGCTGCGGATTTTCACCAATCTTTCGACGCAGTTCATCAGGCGATATAAACGGGATTTCCGCAGCGGCAAATTCATTGAAAATATTGACCGCACTTTTGTTca  >  1:234747/1‑250 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                       AAACCGATTCGATTTGATAAGGCGGAATCTGTTCTTCGTAAGAAATCTGAATCTGTTTGGCAAGGTTGGCATCGTAACGCGGTTGCGTCAGACGATCTTCGCAATCACACAACAACAATTCATCAATAAAGCGCTCCAGTTTGTCATCCGCCTGCCAACGTAACGGGCTGAACAATTCAAAATGCCGCAAAGTGCGGTCGGTTTTCGTCATGAATTTTAACAAGAAGCCCCTGCCCGTGCCTTCGTAGCTGTGAATGGTGGTCGTCAGCACAATACGTTTAAAGGCAGTCGTTAAATCAAACAGCGTATCCAACGGAATCATCGCCGCTTCGTCCACAAATAACCACGAATCCGCAAATTGCTGTGGATTTTCACCAACCTTTTGGCGCAGTTCATCAGGTGGCATAAAGGGGATTTCGACACCGGAAAATTCATTGAAAATCTTGACCGCACTTTTGTTCG  >  1/1474991‑1475452

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑