Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	212,204	C→T	E4K (GAA→AAA)	unknown ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	212,204	0	C	T	100.0%	10.2 / NA	6	E4K (GAA→AAA)	unknown	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (3/3);  total (3/3)

AAGTAAATCTTCAAGATTTATGAGTAAGAAATTAACCGCACTTTTAGAGTAGAACATCAAATCAGAAAGCCGAATTCATTTCGATGAGTTCGGTTTTTTATTGCCCGTAATTAGGGTATTTCTTCTTCGATAAAATATTCAAATAAAAATAATTCTTTTTTGTAGCATAAAATGTAAATATACCTCTTTATAGTTATATATTCCTGCTTCATTTAATTTTCCCCTTTAATGATATTTTTATAATAATAAATAGGTAGTTGACACATTTTAGGAGAAATTTTCATGCTACAAAATTTCACTAAAATAGAAAATTAAATTGGAAAAATTGATCAAAAACAAGAAAATGTAAAATAACCATGGAATGAGCGCGGATATTTGGTTATGTTAAACTCGTTTAACCCCCTTTTATACCTTAT  >  1/212002‑212417                                                                                                                                                                                                           |                                                                                                                                                                                                                      aaGTAAATCTTCAAGATTTATGAGTAAGAAATTAACCGCACTTTTAGAGTAGAACATCAAATCAGAAAGCCGAATTCATTTCGATGAGTTCGGTTTTTTATTGCCCGTAATTAGGGTATTTCTTCTTCGATAAAATATTCAAATAAAAATAATTCTTTTTTGTAGCATAAAATGTAAATATACCTCTTTATAGTTATATATTTCTGCTTCATTTAATTTTCCCCTTTAATGATATTTTTATACTaataaat                                                                                                                                                                       <  1:195623/251‑1 (MQ=255) aaGTAAATCTTCAAGATTTATGAGTAAGAAATTAACCGCACTTTTAGAGTAGAACATCAAATCAGAAAGCCGAATTCATTTCGATGAGTTCGGTTTTTTATTGCCCGTAATTAGGGTATTTCTTCTTCGATAAAATATTCAAATAAAAATAATTCTTTTTTGTAGCATAAAATGTAAATATACCTCTTTATAGTTATATATTTCTGCTTCATTTAATTTTCCCCTTTAATGATATTTTTATACTaataaat                                                                                                                                                                       <  1:792396/251‑1 (MQ=255)                                                                                                            tAATTAGGGTATTTCTTCTTCGATAAAATATTCAAATAAAAATAATTCTTTTTTGTAGCATAAAATGTAAATATACCTCTTTATAGTTATATATTTCTGCTTCATTTAATTTTCCCCTTTAATGATATTTTTATACTAATAAATAGGTAGTTGACACATTTTAGGAGAAATTTTCATGCTACAAAATTTCACTAAAATAGAAAATTAAATTGGAAAAATTGATCAAAAACAAGAAAATGTAAAATAACCAt                                                            >  1:217793/1‑251 (MQ=255)                                                                                                                                                      taatTCTTTTTTGTAGCATAAAATGTAAATATACCTCTTTATAGTTATATATTTCTGCTTCATTTAATTTTCCCCTTTAATGATATTTTTATACTAATAAATAGGTAGTTGACACATTTTAGGAGAAATTTTCATGCTACAAAATTTCACTAAAATAGAAAATTAAATTGGAAAAATTGATCAAAAACAAGAAAATGTAAAATAACCATGGAATGAGCGCGGATATTTGGTTATGTTAAACTCGTTTAAcc                  <  1:813777/251‑1 (MQ=255)                                                                                                                                                                            aTGTAAATATACCTCTTTATAGTTATATATTTCTGCTTCATTTAATTTTCCCCTTTAATGATATTTTtatac                                                                                                                                                                               >  1:485576/1‑71 (MQ=255)                                                                                                                                                                                                 gTTATATATTTCTGCTTCATTTAATTTTCCCCTTTAATGATATTTTTATACTAATAAATAGGTAGTTGACACATTTTAGGAGAAATTTTCATGCTACAAAATTTCACTAAAATAGAAAATTAAATTGGAAAAATTGATCAAAAACAAGAAAATGTAAAATAACCATGGAATGAGCGCGGATATTTGGTTATGTTAAACTCGTTTAACCCCCTTTTATACCTtat  >  1:273020/1‑224 (MQ=255)                                                                                                                                                                                                           |                                                                                                                                                                                                                      AAGTAAATCTTCAAGATTTATGAGTAAGAAATTAACCGCACTTTTAGAGTAGAACATCAAATCAGAAAGCCGAATTCATTTCGATGAGTTCGGTTTTTTATTGCCCGTAATTAGGGTATTTCTTCTTCGATAAAATATTCAAATAAAAATAATTCTTTTTTGTAGCATAAAATGTAAATATACCTCTTTATAGTTATATATTCCTGCTTCATTTAATTTTCCCCTTTAATGATATTTTTATAATAATAAATAGGTAGTTGACACATTTTAGGAGAAATTTTCATGCTACAAAATTTCACTAAAATAGAAAATTAAATTGGAAAAATTGATCAAAAACAAGAAAATGTAAAATAACCATGGAATGAGCGCGGATATTTGGTTATGTTAAACTCGTTTAACCCCCTTTTATACCTTAT  >  1/212002‑212417

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑