Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,475,027	C→T	E351K (GAA→AAA)	unknown ←	tRNA cytosine(34) acetyltransferase (EC 2.3.1.193)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,475,027	0	C	T	100.0%	191.1 / NA	70	E351K (GAA→AAA)	unknown	tRNA cytosine(34) acetyltransferase (EC 2.3.1.193)
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (40/30);  total (40/30)

CCAAATTACCACGTGGACGACGTTGTCCGCGCCGAATTTTACGAATTAATGCGTCGTCACCAAATTTGCCCTCAGGCACCGCCCACACACAACCCAACAAGGCCTCCTGCGTCTGCGCAAGATAAAATTGTTGTCGCGGCGCATCCAATAATCGACGTAAATCCAACGGCGAGGTTCGGTAATGCGCCAAGGTCAGCAAGCCATAAACCGATTCGATTTGATAAGGCGGAATCTGTTCTTCGTAAGAAATCTGAATCTGTTTGGCAAGGTTGGCATCGTAACGCGGTTGCGTCAGACGATCTTCGCAATCACACAACAACAATTCATCAATAAAGCGCTCCAGTTTGTCATCCGCCTGCCAACGTAACGGGCTGAACAATTCAAAATGCCGCAAAGTGCGGTCGGTTTTCGTCATGAATTTTAACAAGAAGCCCCTGCCCGTGCCTTCGTAGCTGTGAATGGTGGTCGTCAGCACAATAC  >  1/1474787‑1475266                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                ccaaattgccgcgcaggcgacgTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTCGCGGTGCATCCAATAATCGACGTAAATCCAACGGCGAGGTGCGGTAATGCGCCAAAGTCAGCAAGCCATAAACCGATTCGATTTGATTAGGCGGAATCCGTTCTTTGTAAGAaat                                                                                                                                                                                                                                        >  1:28453/18‑250 (MQ=255) ccaaattgccgcgcaggcgacgTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTCGCGGTGCATCCAATAATCGACGTAAATCCAACGGCGAGGTGCGGTAATGCGCCAAAGTCAGCAAGCCATAAACCGATTCGATTTGATTAGGCGGAATCCGTTCTTTGTAAGAaat                                                                                                                                                                                                                                        >  1:130677/18‑250 (MQ=255)                                tcGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTCGCGGTGCATCCAATAATCGACGTAAATCCAACGGCGAGGTGCGGTAATGCGCCAAAGTCAGCAAGCCATAAACCGATTCGATTTGATTAGGCGGAATCCGTTCTTTGTAAGAAATCTGAATCTGTTCGGCAAGGttagcgtcataac                                                                                                                                                                                                        <  1:177839/250‑12 (MQ=255)                                   aaTTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTCGCGGTGCATCCAATAATCGACGTAAATCCAACGGCGAGGTGCGGTAATGCGCCAAAGTCAGCAAGCCATAAACCGATTCGATTTGATTAGGCGGAATCCGTTCTTTGTAAGAAATCTGAATCTGTTCGGCAAGGttagcgtcataacac                                                                                                                                                                                                      <  1:283929/250‑14 (MQ=255)                                   aaTTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTCGCGGTGCATCCAATAAGCGACGTAAATCCAACGGCGAGGTGCGGTAATGCGCCAAAGTCAGCAAGCCATAAACCGATTCGATTTGATTAGGCGGAATCCGTTCTTTGTAAGAAATCTGAATCTGTTCGGCAAGGttagcgtcataacac                                                                                                                                                                                                      <  1:890573/250‑14 (MQ=255)                                                        atcctcaaatcctccttCAGGCACCGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTCGCGGTGCATCCAATAATCGACGTAAATCCAACGGCGAGGTGCGGTAATGCGCCAAAGTCAGCAAGCCATAAACCGATTCGATTTGATTAGGCGGAATCCGTTCTTTGTAAGAAATCTGAATCTGTTCGGCAAGGTTAGCGTCATAACACGGTTGCGGCAGACGATCTTCg                                                                                                                                                                                 >  1:674652/17‑250 (MQ=255)                                                        atcctcaaatcctccttCAGGCACCGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTCGCGGTGCATCCAATAATCGACGTAAATCCAACGGCGAGGTGCGGTAATGCGCCAAAGTCAGCAAGCCATAAACCGATTCGATTTGATTAAGCGGAATCCGTTCTTTGTAAGAAATCTGAATCTGTTCGGCAAGGTTAGCGTCATAACACGGTTGCGGCAGACGATCTTCg                                                                                                                                                                                 >  1:443662/17‑250 (MQ=255)                                                             caaatcctccttCAGGCACCGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTCGCGGTGCATCCAATAATCGACGTAAATCCAACGGCGAGGTGCGGTAATGCGCCAAAGTCAGCAAGCCATAAACCGATTCGATTTGATTAGGCGGAATCCGTTCTTTGTAAGAAATCTGAATCTGTTCGGCAAGGttagcgt                                                                                                                                                                                                              >  1:695858/12‑211 (MQ=255)                                                                atcctccttCAGGCACCGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTCGCGGTGCATCCAATAATCGACGTAAATCCAACGGCGAGGTGCGGTAATGCGCCAAAGTCAGCAAGCCATAAACCGATTCGATTTGATTAGGCGGAATCCGTTCTTTGTAAGAAATCTGAATCTGTTCGGCAAGGTTAGCGTCATAACACGGTTGCGGCAGACGATCTTCGCAATcacac                                                                                                                                                                        <  1:406502/243‑1 (MQ=255)                                                                      cttCAGGCACCGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTCGCGGTGCATCCAATAATCGACGTAAATCCAACGGCGAGGTGCGGTAATGCGCCAAAGTCAGCAAGCCATAAACCGATTCGATTTGATTAGGCGGAATCCGTTCTTTGTAAGAAATCTGAATCTGTTCGGCAAGGTTAGCGTCATAACACGGTTGCGGCAGACGAt                                                                                                                                                                                      <  1:823891/229‑1 (MQ=255)                                                                      cttCAGGCACCGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTCGCGGTGCATCCAATAATCGACGTAAATCCAACGGCGAGGTGCGGTAATGCGCCAAAGTCAGCAAGCCATAAACCGATTCGATTTGATTAGGCGGAATCCGTTCTTTGTAAGAAATCTGAATCTGTTCGGCAAGGTTAGCGTCATAACACGGTTGCGGCAGACGATCTTCGCaa                                                                                                                                                                              <  1:342096/237‑1 (MQ=255)                                                                      cttCAGGCACCGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTCGCGGTGCATCCAATAATCGACGTAAATCCAACGGCGAGGTGCGGTAATGCGCCAAAGTCAGCAAGCCATAAACCGATTCGATTTGATTAGGCGGAATCCGTTCTTTGTAAGAAATCTGAATCTGTTCGGCAAGGTTAGCGTCATAACACGGTTGCGGCAGACGATCTTCGCAATCACacaacaa                                                                                                                                                                   >  1:619874/3‑250 (MQ=255)                                                                       ttCAGGCACCGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTCGCGGTGCATCCAATAATCGACGTAAATCCAACGGCGAGGTGCGGTAATGCGCCAAAGTCAGCAAGCCATAAACCGATTCGATTTGATTAGGCGGAATCCGTTCTTTGTAAGAAATCTGAATCTGTTCGGCAAGGttagcgtcataacac                                                                                                                                                                                                      >  1:252535/2‑201 (MQ=255)                                                                       ttCAGGCACCGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTCGCGGTGCATCCAATAATCGACGTAAATCCAACGGCGAGGTGCGGTAATGCGCCAAAGTCAGCAAGCCATAAACCGATTCGATTTGATTAGGCGGAATCCGTTCTTTGTAAGAAATCTGAATCTGTTCGGCAAGGTTAGCGTCATAACACGGTTGCGGCAGACGATCTTCGCAATCACacaacaaca                                                                                                                                                                 >  1:175357/2‑251 (MQ=255)                                                                               ccGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTCGCGGTGCATCCAATAATCGACGTAAATCCAACGGCGAGGTGCGGTAATGCGCCAAAGTCAGCAAGCCATAAACCGATTCGATTTGATTAGGCGGAATCCGTTCTTTGTAAGAAATCTGAATCTGTTCGGCAAGGTTAGCGTCATAACACGGTTGCGGCAGACGATCTTCGCAATCACACAACAACAATtcgtca                                                                                                                                                         >  1:607949/1‑251 (MQ=255)                                                                                  cccACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTCGCGGTGCATCCAATAATCGACGTAAATCCAACGGCGAGGTGCGGTAATGCGCCAAAGTCAGCAAGCCATAAACCGATTCGATTTGATTAGGCGGAATCCGTTCTTTGTAAGAAATCTGAATCTGTTCGGCAAGGTTAGCGTCATAACACGGTTGCGGCAGACGATCTTCGCAATCACACAACAACAATTCGTCAATa                                                                                                                                                      >  1:278758/1‑251 (MQ=255)                                                                                     acacacaGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTCGCGGTGCATCCAATAATCGACGTAAATCCAACGGCGAGGTGCGGTAATGCGCCAAAGTCAGCAAGCCATAAACCGATTCGATTTGATTAGGCGGAATCCGTTCTTTGTAAGAAATCTGAATCTGTTCGGCAAGGTTAGCGTCATAACACGGTTGCGGCAGACGATCTTCGCAATCACACAACAACAATTCGTCAATAAAg                                                                                                                                                   >  1:39237/1‑251 (MQ=255)                                                                                      cacacaGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTCGCGGTGCATCCAATAATCGACGTAAATCCAACGGCGAGGTGCGGTAATGCGCCAAAGTCAGCAAGCCATAAACCGATTCGATTTGATTAGGCGGAATCCGTTCTTTGTAAGAAATCTGAATCTGTTCGGCAAGGttagcgt                                                                                                                                                                                                              <  1:290264/191‑6 (MQ=255)                                                                                      cacacaGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTCGCGGTGCATCCAATAATCGACGTAAATCCAACGGCGAGGTGCGGTAATGCGCCAAAGTCAGCAAGCCATAAACCGATTCGATTTGATTAGGCGGAATCCGTTCTTTGTAAGAAATCTGAATCTGTTCGGCAAGGTTAGCGTCATAACACGGTTGCGGCAGACGATCTTCGCAATCACACAACAACAATTCGTCAATAAAg                                                                                                                                                   >  1:420895/1‑250 (MQ=255)                                                                                            gcccaacagggtttcccgcgtttgtgcAAAATAAAATTGTTGTCGCGGTGCATCCAATAATCGACGTAAATCCAACGGCGAGGTGCGGTAATGCGCCAAAGTCAGCAAGCCATAAACCGATTCGATTTGATTAGGCGGAATCCGTTCTTTGTAAGAAATCTGAATCTGTTCGGCAAGGTTAGCGTCATAACACGGTTGCGGCAGACGATCTTCGCAATCACACAACAACAATTCGTCAATAAAGCGCTCCa                                                                                                                                            >  1:527982/26‑251 (MQ=255)                                                                                            gcccaacagggtttcccgcgtttgtgcAAAATAAAATTGTTGTCGCGGTGCATCCAATAATCGACGTAAATCCAACGGCGAGGTGCGGTAATGCGCCAAAGTCAGCAAGCCATAAACCGATTCGATTTGATTAGGCGGAATCCGTTCTTTGTAAGAAATCTGAATCTGTTCGGCAAGGTTAGCGTCATAACACGGTTGCGGCAGACGATCTTCGCAATCACACAACAACAATTCGTCAATAAAGCGCTCCa                                                                                                                                            >  1:367894/26‑251 (MQ=255)                                                                                               caacagggtttcccgcgtttgtgcAAAATAAAATTGTTGTCGCGGTGCATCCAATAATCGACGTAAATCCAACGGCGAGGTGCGGTAATGCGCCAAAGTCAGCAAGCCATAAACCGATTCGATTTGATTAGGCGGAATCCGTTCTTTGTAAGAAATCTGAATCTGTTCGGCAAGGTTAGCGTCATAACACGGTTGCGGCAGACGATCTTCGCAATCACACAACAACAATTCGTCAATAAAGCGCTCCAGtt                                                                                                                                         >  1:604455/23‑251 (MQ=255)                                                                                                     ggtttcccgcgtttgtgcAAAATAAAATTGTTGTCGCGGTGCATCCAATAATCGACGTAAATCCAACGGCGAGGTGCGGTAATGCGCCAAAGTCAGCAAGCCATAAACCGATTCGATTTGATTAGGCGGAATCCGTTCTTTGTAAGAAATCTGAATCTGTTCGGCAAGGTTAGCGTCATAACACGGTTGCGGCAGACGATCTTCGCAATCACACAACAACAATTCGTCAATAAAGCGCTCCAGTTTGTCa                                                                                                                                    <  1:428935/234‑1 (MQ=255)                                                                                                                      caaaaTAAAATTGTTGTCGCGGTGCATCCAATAATCGACGTAAATCCAACGGCGAGGTGCGGTAATGCGCCAAAGTCAGCAAGCCATAAACCGATTCGATTTGATTAGGCGGAATCCGTTCTTTGTAAGAAATCTGAATCTGTTCGGCAAGGTTAGCGTCATAACACGGTTGCGGCAGACGATCTTCGCAATCACACAACAACAATTCGTCAATAAAGCGCTCCAGTTTGTCATCCGCCTGCCAACGTag                                                                                                                   <  1:11101/246‑2 (MQ=255)                                                                                                                          aTAAAATTGTTGTCGCGGTGCATCCAATAATCGACGTAAATCCAACGGCGAGGTGCGGTAATGCGCCAAAGTCAGCAAGCCATAAACCGATTCGATTTGATTAGGCGGAATCCGTTCTTTGTAAGAAATCTGAATCTGTTCGGCAAGGTTAGCGTCATAACACGGTTGCGGCAGACGATCTTCGCAATCACACAACAACAATTCGTCAATAAAGCGCTCCAGTTTGTCATCCGc                                                                                                                               <  1:284801/234‑1 (MQ=255)                                                                                                                          aTAAAATTGTTGTCGCGGTGCATCCAATAATCGACGTAAATCCAACGGCGAGGTGCGGTAATGCGCCAAAGTCAGCAAGCCATAAACCGATTCGATTTGATTAGGCGGAATCCGTTCTTTGTAAGAAATCTGAATCTGTTCGGCAAGGTTAGCGTCATAACACGGTTGCGGCAGACGATCTTCGCAATCACACAACAACAATTCGTCAATAAAGCGCTCCAGTTTGTCATCCGCCTGCCAACGTAGCGGGc                                                                                                              <  1:229495/251‑1 (MQ=255)                                                                                                                                       cgcgGTGCATCCAATAATCGACGTAAATCCAACGGCGAGGTGCGGTAATGCGCCAAAGTCAGCAAGCCATAAACCGATTCGATTTGATTAGGCGGAATCCGTTCTTTGTAAGAAATCTGAATCTGTTCGGCAAGGTTAGCGTCATAACACGGTTGCGGCAGACGATCTTCGCAATCACACAACAACAATTCGTCAATAAAGCGCTCCAGTTTGTCATCCGCCTGCCAACGTAGCGGGCTGAACAATTCaaa                                                                                                 <  1:437937/251‑1 (MQ=255)                                                                                                                                          ggtgcATCCAATAATCGACGTAAATCCAACGGCGAGGTGCGGTAATGCGCCAAAGTCAGCAAGCCATAAACCGATTCGATTTGATTAGGCGGAATCCGTTCTTTGTAAGAAATCTGAATCTGTTCGGCAAGGTTAGCGTCATAACACGGTTGCGGCAGACGATCTTCGCAATCACACAACAACAATTCGTCAATAAAGCGCTCCAGTTTGt                                                                                                                                      >  1:327108/4‑211 (MQ=255)                                                                                                                                               aTCCAATAATCGACGTAAATCCAACGGCGAGGTGCGGTAATGCGCCAAAGTCAGCAAGCCATAAACCGATTCGATTTGATTAGGCGGAATCCGTTCTTTGTAAGAAATCTGAATCTGTTCGGCAAGGTTAGCGTCATAACACGGTTGCGGCAGACGATCTTCGCAATCACACAACAACAATTCGTCAATAAAGCGCTCCAGTTTGTCATCCGCCTGCCAACGTAGCGGGCTGAAc                                                                                                         <  1:535960/235‑1 (MQ=255)                                                                                                                                               aTCCAATAATCGACGTAAATCCAACGGCGAGGTGCGGTAATGCGCCAAAGTCAGCAAGCCATAAACCGATTCGATTTGATTAGGCGGAATCCGTTCTTTGTAAGAAATCTGAATCTGTTCGGCAAGGTTAGCGTCATAACACGGTTGCGGCAGACGATCTTCGCAATCACACAACAACAATTCGTCAATAAAGCGCTCCAGTTTGTCATCCGCCTGCCAACGTAGCGGGCTGAACAATTCAAAATGCCgaa                                                                                         >  1:477740/1‑249 (MQ=255)                                                                                                                                                tCCAATAATCGACGTAAATCCAACGGCGAGGTGCGGTAATGCGCCAAAGTCAGCAAGCCATAAACCGATTCGATTTGATTAGGCGGAATCCGTTCTTTGTAAGAAATCTGAATCTGTTCGGCAAGGTTAGCGTCATAACACGGTTGCGGCAGACGATCTTCGCAATCACCCAACAACAATTCGTCAATAAAGCGCTCCAGTTTGTCATCCGCCTGCCAACGTAGCGGGCTGAACAATTCAAAATGCCgaaa                                                                                        >  1:445058/1‑248 (MQ=255)                                                                                                                                                  cAATAATCGACGTAAATCCAACGGCGAGGTGCGGTAATGCGCCAAAGTCAGCAAGCCATAAACCGATTCGATTTGATTAGGCGGAATCCGTTCTTTGTAAGAAATCTGAATCTGTTCGGCAAGGTTAGCGTCATAACACGGTTGCGGCAGACGATCTTCGCAATCACACAACAACAATTCGTCAATAAAGCGCTCCAGTTTGTCATCCGCCTGCCAACGTAGCGGGCTGAACAATTCAAAATGCCGAAAAg                                                                                      <  1:823289/251‑1 (MQ=255)                                                                                                                                                           aCGTAAATCCAACGGCGAGGTGCGGTAATGCGCCAAAGTCAGCAAGCCATAAACCGATTCGATTTGATTAGGCGGAATCCGTTCTTTGTAAGAAATCTGAATCTGTTCGGCAAGGTTAGCGTCATAACACGGTTGCGGCAGACGATCTTCGCAATCACACAACAACAATTCGTCAATAAAGCGCTCCAGTTTGTCATCCGCCTGCCAACGTAGCGGGCTGAACAATTCAAAATGCCGAAAAGTGcggtcgg                                                                             >  1:888508/1‑251 (MQ=255)                                                                                                                                                                 aTCCAACGGCGAGGTGCGGTAATGCGCCAAAGTCAGCAAGCCATAAACCGATTCGATTTGATTAGGCGGAATCCGTTCTTTGTAAGAAATCTGAATCTGTTCGGCAAGGTTAGCGTCATAACACGGTTGCGGCAGACGATCTTCGCAATCACACAACAACAATTCGTCAATAAAGCGCTCCAGTTTGTCATCCGCCTGCCAACGTAGCGGGCTGAACAATTCAAAATGCCGAAAAGTGCGGTCGGTGTTCg                                                                       >  1:12318/1‑251 (MQ=255)                                                                                                                                                                 aTCCAACGGCGAGGTGCGGTAATGCGCCAAAGTCAGCAAGCCATAAACCGATTCGATTTGATTAGGCGGAATCCGTTCTTTGTAAGAAATCTGAATCTGTTCGGCAAGGTTAGCGTCATAACACGGTTGCGGCAGACGATCTTCGCAATCACACAACAACAATTCGTCAATAAAGCGCTCCAGTTTGTCATCCGCCTGCCAACGTAGCGGGCTGAACAATTCAAAATGCCGAAAAGTGCGGTCGGTGTTCg                                                                       >  1:831458/1‑251 (MQ=255)                                                                                                                                                                   ccAACGGCGAGGTGCGGTAATGCGCCAAAGTCAGCAAGCCATAAACCGATTCGATTTGATTAGGCGGAATCCGTTCTTTGTAAGAAATCTGAATCTGTTCGGCAAGGTTAGCGTCATAACACGGTTGCGGCAGACGATCTTCGCAATCACACAACAACAATTCGTCAATAAAGCGCTCCAGTTTGTCATCCGCCTGCCAACGTAGCGGGCTGAACAATTCAAAATGCCGAAAAGTGCGGTCGGTGTTCgct                                                                     <  1:151873/251‑3 (MQ=255)                                                                                                                                                                   ccAACGGCGAGGTGCGGTAATGCGCCAAAGTCAGCAAGCCATAAACCGATTCGATTTGATTAGGCGGAATCCGTTCTTTGTAAGAAATCTGAATCTGTTCGGCAAGGTTAGCGTCATAACACGGTTGCGGCAGACGATCTTCGCAATCACACAACAACAATTCGTCAATAAAGCGCTCCAGTTTGTCATCCGCCTGCCAACGTAGCGGGCTGAACAATTCAAAATGCCGAAAAGTGCGGTCGGTGTTCgct                                                                     <  1:624709/251‑3 (MQ=255)                                                                                                                                                                              gtgcGGTAATGCGCCAAAGTCAGCAAGCCATAAACCGATTCGATTTGATTAGGCGGAATCCGTTCTTTGTAAGAAATCTGAATCTGTTCGGCAAGGTTAGCGTCATAACACGGTTGCGGCAGACGATCTTCGCAATCACACAACAACAATTCGTCAATAAAg                                                                                                                                                   >  1:189234/4‑162 (MQ=255)                                                                                                                                                                              gtgcGGTAATGCGCCAAAGTCAGCAAGCCATAAACCGATTCGATTTGATTAGGCGGAATCCGTTCTTTGTAAGAAATCTGAATCTGTTCGGCAAGGTTAGCGTCATAACACGGTTGCGGCAGACGATCTTCGCAATCACACAACAACAATTCGTCAATAAAGCGCTCCAGTTTGTCATCCGCCTGCCAACGTAGCGGGCTGAACAATTCAAAATGCCGAAAAg                                                                                      <  1:514904/220‑1 (MQ=255)                                                                                                                                                                              gtgcGGTAATGCGCCAAAGTCAGCAAGCCATAAACCGATTCGATTTGATTAGGCGGAATCCGTTCTTTGTAAGAAATCTGAATCTGTTCGGCAAGGTTAGCGTCATAACACGGTTGCGGCAGACGATCTTCGCAATCACACAACAACAATTCGTCAATAAAGCGCTCCAGTTTGTCATCCGCCTGCCAACGTAGCGGGCTGAACAATTCAAAATGCCGAAAAGTGCGGTCGGTGTTCGCTATGAATTTTaa                                                          >  1:75896/4‑251 (MQ=255)                                                                                                                                                                              gtgcGGTAATGCGCCAAAGTCAGCAAGCCATAAACCGATTCGATTTGATTAGGCGGAATCCGTTCTTTGTAAGAAATCTGAATCTGTTCGGCAAGGTTAGCGTCATAACACGGTTGCGGCAGACGATCTTCGCAATCACACAACAACAATTCGTCAATAAAGCGCTCCAGTTTGTCATCCGCCTGCCAACGTAGCGGGCTGAACAATTCAAAATGCCGAAAAGTGCGGTCGGTGTTCGCTATGAATTTTaa                                                          >  1:140583/4‑251 (MQ=255)                                                                                                                                                                               tgcGGTAATGCGCCAAAGTCAGCAAGCCATAAACCGATTCGATTTGATTAGGCGGAATCCGTTCTTTGTAAGAAATCTGAATCTGTTCGGCAAGGTTAGCGTCATAACACGGTTGCGGCAGACGATCTTCGCAATCACACAACAACAATTCGTCAATAAAGCGCTCCAGTTTGTCATCCGCCTGCCAACGTAGCGGGCTGAACAATTCAAAATGCCGAAAAGTGCGGTCGGTGTTCGCTATGAATTTTAAc                                                         <  1:326311/249‑1 (MQ=255)                                                                                                                                                                                gcGGTAATGCGCCAAAGTCAGCAAGCCATAAATCGATTCGATTTGATTAGGCGGAATCCGTTCTTTGTAAGAAATCTGAATCTGTTCGGCAAGGTTAGCGTCATAACACGGTTGCGGCAGACGATCTTCGCAATCACACAACAACAATTCGTCAATAAAGCGCTCCAGTTTGTCATCCGCCTGCCAAAGTAGCGGGCTGAACAATTCAAAATGCCGAAAAGTGCGGTCGGTGTTCGCTATGAATTTTAACa                                                        >  1:386945/2‑251 (MQ=255)                                                                                                                                                                                 cGGTAATGCGCCAAAGTCAGCAAGCCATAAACCGATTCGATTTGATTAGGCGGAATCCGTTCTTTGTAAGAAATCTGAATCTGTTCGGCAAGGTTAGCGTCATAACACGGTTGCGGCAGACGATCTTCGCAATCACACAACAACAATTCGTCAATAAAGCGCTCCAGTTTGTCATCCGCCTGCCAACGTAGCGGGCTGAACAATTCAAAATGCCGAAAAGTGCGGTCGGTGTTCGCTATGAATTTTAACa                                                        <  1:480086/250‑1 (MQ=255)                                                                                                                                                                                   gTAATGCGCCAAAGTCAGCAAGCCATAAACCGATTCGATTTGATTAGGCGGAATCCGTTCTTTGTAAGAAATCTGAATCTGTTCGGCAAGGTTAGCGTCATAACACGGt                                                                                                                                                                                                   >  1:754798/1‑109 (MQ=255)                                                                                                                                                                                          gcCAAAGTCAGCAAGCCATAAACCGATTCGATTTGATTAGGCGGAATCCGTTCTTTGTAAGAAATCTGAATCTGTTCGGCAAGGTTAGCGTCATAACACGGTTGCGGCAGACGATCTTCGCAATCACACAACAACAATTCGTCAATAAAGCGCTCCAGTTTGTCATCCGCCTGCCAACGTAGCGGGCTGAACAATTCAAAATGCCGAAAAGTGCGGTCGGTGTTCGCTATGAATTTTAACAAGAAGccc                                                >  1:810904/1‑249 (MQ=255)                                                                                                                                                                                                gTCAGCAAGCCATAAACCGATTCGATTTGATTAGGCGGAATCCGTTCTTTGTAAGAAATCTGAATCTGTTCGGCAAGGTTAGCGTCATAACACGGTTGCGGCAGACGATCTTCGCAATCACACAACAACAATTCGTCAATAAAGCGCTCCAGTTTGTCATCCGCCTGCCAACGTAGCGGGCTGAACAATTCAAAATGCCGAAAAGTGCGGTCGGTGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGt                                        >  1:291446/1‑251 (MQ=255)                                                                                                                                                                                                  cAGCAAGCCATAAACCGATTCGATTTGATTAGGCGGAATCCGTTCTTTGTAAGAAATCTGAATCTGTTCGGCAAGGTTAGCGTCATAACACGGTTGCGGCAGACGATCTTCGCAATCACACAACAACAATTCGTCAATAAAGCGCTCCAGTTTGTCATCCGCCTGCCAACGTAGCGGGCTGAACAATTCAAAATGCCGAAAAGTGCGGTCGGTGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGta                                       >  1:508527/1‑249 (MQ=255)                                                                                                                                                                                                     cAAGCCATAAACCGATTCGATTTGATTAGGCGGAATCCGTTCTTTGTAAGAAATCTGAATCTGTTCGGCAAGGTTAGCGTCATAACACGGTTGCGGCAGACGATCTTCGCAATCACACAACAACAATTCGTCAATAAAGCGCTCCAGTTTGTCATCCGCCTGCCAACGTAGCGGGCTGAACAATTCAAAATGCCGAAAAGTGCGGTCGGTGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGtacc                                     <  1:573906/249‑4 (MQ=255)                                                                                                                                                                                                     cAAGCCATAAACCGATTCGATTTGATTAGGCGGAATCCGTTCTTTGTAAGAAATCTGAATCTGTTCGGCAAGGTTAGCGCCATAACACGGTTGCGGCAGACGATCTTCGCAATCACACAACAACAATTCGTCAATAAAGCGCTCCAGTTTGTCATCCGCCTGCCAACGTAGCGGGCTGAACAATTCAAAATGCCGAAAAGTGCGGTCGGTGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGtacc                                     <  1:443813/249‑4 (MQ=255)                                                                                                                                                                                                        gCCATAAACCGATTCGATTTGATTAGGCGGAATCCGTTCTTTGTAAGAAATCTGAATCTGTTCGGCAAGGTTAGCGTCATAACACGGTTGCGGCAGACGATCTTCGCAATCACACAACAACAATTCGTCAATAAAGCGCTCCAGTTTGTCATCCGCCTGCCAACGTAGCGGGCTGAACAATTCAAAATGCCGAAAAg                                                                                      >  1:203440/1‑197 (MQ=255)                                                                                                                                                                                                        gCCATAAACCGATTCGATTTGATTAGGCGGAATCCGTTCTTTGTAAGAAATCTGAATCTGTTCGGCAAGGTTAGCGTCATAACACGGTTGCGGCAGACGATCTTCGCAATCACACAACAACAATTCGTCAATAAAGCGCTCCAGTTTGTCATCCGCCTGCCAACGTAGCGGGCTGAACAATTCAAAATGCCGAAAAg                                                                                      <  1:876514/197‑1 (MQ=255)                                                                                                                                                                                                        gCCATAAACCGATTCGATTTGATTAGGCGGAATCCGTTCTTTGTAAGAAATCTGAATCTGTTCGGCAAGGTTAGCGTCATAACACGGTTGCGGCAGACGATCTTCGCAATCACACAACAACAATTCGTCAATAAAGCGCTCCAGTTTGTCATCCGCCTGCCAACGTAGCGGGCTGAACAATTCAAAATGCCGAAAAGTGCGGTCGGTGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGt                                >  1:833517/1‑251 (MQ=255)                                                                                                                                                                                                         ccATAAACCGATTCGATTTGATTAGGCGGAATCCGTTCTTTGTAAGAAATCTGAATCTGTTCGGCAAGGTTAGCGTCATAACACGGTTGCGGCAGACGATCTTCGCAATCACACAACAACAATTCGTCAATAAAGCGCTCCAGTTTGTCATCCGCCTGCCAACGTAGCGGGCTGAACAATTCAAAAt                                                                                               >  1:881742/1‑187 (MQ=255)                                                                                                                                                                                                           aTAAACCGATTCGATTTGATTAGGCGGAATCCGTTCTTTGTAAGAAATCTGAATCTGTTCGGCAAGGTTAGCGTCATAACACGGTTGCGGCAGACGATCTTCGCAATCACACAACAACAATTCGTCAATAAAGCGCTCCAGTTTGTCATCCGCCTGCCAACGTAGCGGGCTGAACAATTCAAAATGCCGAAAAGTGCGGTCGGTGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTaac                             <  1:212776/251‑3 (MQ=255)                                                                                                                                                                                                             aaaCCGATTCGATTTGATTAGGCGGAATCCGTTCTTTGTAAGAAATCTGAATCTGTTCGGCAAGGTTAGCGTCATAACACGGTTGCGGCAGACGATCTTCGCAATCACACAACAACAATTCGTCAATAAAGCGCTCCAGTTTGTCATCCGCCTGCCAACGTAGCGGGCTGAACAATTCAAAATGCCGAAAAGTGCGGTCGGTGTTCgct                                                                     >  1:90505/1‑207 (MQ=255)                                                                                                                                                                                                             aaaCCGATTCGATTTGATTAGGCGGAATCCGTTCTTTGTAAGAAATCTGAATCTGTTCGGCAAGGTTAGCGTCATAACACGGTTGCGGCAGACGATCTTCGCAATCACACAACAACAATTCGTCAATAAAGCGCTCCAGTTTGTCATCCGCCTGCCAACGTAGCGGGCTGAACAATTCAAAATGCCGAAAAGTGCGGTCGGTGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACtg                           <  1:807094/251‑1 (MQ=255)                                                                                                                                                                                                                     tcgattTGATTAGGCGGAATCCGTTCTTTGTAAGAAATCTGAATCTGTTCGGCAAGGTTAGCGTCATAACACGGTTGCGGCAGACGATCTTCGCAATCACACAACAACAATTCGTCAATAAAGCGCTCCAGTTTGTCATCCGCCTGCCAACGTAGCGGGCTGAACAATTCAAAATGCCGAAAAGTGCGGTCGGTGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAAtggt                   <  1:103846/251‑1 (MQ=255)                                                                                                                                                                                                                            gattaGGCGGAATCCGTTCTTTGTAAGAAATCTGAATCTGTTCGGCAAGGTTAGCGTCATAACACGGTTGCGGCAGACGATCTTCGCAATCACACAACAACAATTCGTCAATAAAGCGCTCCAGTTTGTCATCCGCCTGCCAACGTAGCGGGCTGAACAATTCAAAATGCCGAAAAGTGCGGTCGGTGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGtac                                      >  1:358157/5‑223 (MQ=255)                                                                                                                                                                                                                            gattaGGCGGAATCCGTTCTTTGTAAGAAATCTGAATCTGTTCGGCAAGGTTAGCGTCATAACACGGTTGCGGCAGACGATCTTCGCAATCACACAACAACAATTCGTCAATAAAGCGCTCCAGTTTGTCATCCGCCTGCCAACGTAGCGGGCTGAACAATTCAAAATGCCGAAAAGTGCGGTCGGTGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAAtggtcgtggt             >  1:499629/5‑244 (MQ=255)                                                                                                                                                                                                                                  gCGGAATCCGTTCTTTGTAAGAAATCTGAATCTGTTCGGCAAGGTTAGCGTCATAACACGGTTGCGGCAGACGATCTTCGCAATCACACAACAACAATTCGTCAATAAAGCGCTCCAGTTTGTCATCCGCCTGCCAACGTAGCGGGCTGAACAATTCAAAATGCCGAAAAGTGCGGTCGGTGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAATGGTCGTGGTCAGcac       <  1:713065/250‑1 (MQ=255)                                                                                                                                                                                                                                  gCGGAATCCGTTCTTTGTAAGAAATCTGAATCTGTTCGGCAAGGTTAGCGTCATAACACGGTTGCGGCAGACGATCTTCGCAATCACACAACAACAATTCGTCAATAAAGCGCTCCAGTTTGTCATCCGCCTGCCAACGTAGCGGGCTGAACAATTCAAAATGCCGAAAAGTGCGGTCGGTGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAATGGTCGTGGTCAGcac       <  1:408326/250‑1 (MQ=255)                                                                                                                                                                                                                                  gCGGAATCCGTTCTTTGTAAGAAATCTGAATCTGTTCGGCAAGGTTAGCGTCATAACACGGTTGCGGCAGACGATCTTCGCAATCACACAACAACAATTCGTCAATAAAGCGCTCCAGTTTGTCATCCGCCTGCCAACGTAGCGGGCTGAACAATTCAAAATGCCGAAAAGTGCGGTCGGTGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAATGGTCGTGGTCAGcac       <  1:328430/250‑1 (MQ=255)                                                                                                                                                                                                                                  gCGGAATCCGTTCTTTGTAAGAAATCTGAATCTGTTCGGCAAGGTTAGCGTCATAACACGGTTGCGGCAGACGATCTTCGCAATCACACAACAACAATTCGTCAATAAAGCGCTCCAGTTTGTCATCCGCCTGCCAACGTAGCGGGCTGAACAATTCAAAATGCCGAAAAGTGCGGTCGGTGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAATGGTCGTGGTCAGcac       <  1:109686/250‑1 (MQ=255)                                                                                                                                                                                                                                     gAATCCGTTCTTTGTAAGAAATCTGAATCTGTTCGGCAAGGTTAGCGTCATAACACGGTTGCGGCAGACGATCTTCGCAATCACacaacaac                                                                                                                                                                  >  1:3000/1‑92 (MQ=255)                                                                                                                                                                                                                                     gAATCCGTTCTTTGTAAGAAATCTGAATCTGTTCGGCAAGGTTAGCGTCATAACACGGTTGCGGCAGACGATCTTCGCAATCACACAACAACAATtcgtc                                                                                                                                                          >  1:827134/1‑97 (MQ=255)                                                                                                                                                                                                                                     gAATCCGTTCTTTGTAAGAAATCTGAATCTGTTCGGCAAGGTTAGCGTCATAACACGGTTGCGGCAGACGATCTTCGCAATCACACAACAACAATTCGTCAATAAAGCGCTCCAGTTTGTCATCCGc                                                                                                                               >  1:874650/1‑127 (MQ=255)                                                                                                                                                                                                                                     gAATCCGTTCTTTGTAAGAAATCTGAATCTGTTCGGCAAGGTTAGCGTCATAACACGGTTGCGGCAGACGATCTTCGCAATCACACAACAACAATTCGTCAATAAAGCGCTCCAGTTTGTCATCCGCCTGCCAACGTAGCGGGCTGAACAATTCAAAATGCCGAAAAGTGCGGTCGGTGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAATGGTCGTGGTCAGCACaa     >  1:535326/1‑249 (MQ=255)                                                                                                                                                                                                                                     gAATCCGTTCTTTGTAAGAAATCTGAATCTGTTCGGCAAGGTTAGCGTCATAACACGGTTGCGGCAGACGATCTTCGCAATCACACAACAACAATTCGTCAATAAAGCGCTCCAGTTTGTCATCCGCCTGCCAACGTAGCGGGCTGAACAATTCAAAATGCCGAAAAGTACGGTCGGTGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAATGGTCGTGGTCAGCACaa     >  1:447481/1‑249 (MQ=255)                                                                                                                                                                                                                                      aaTCCGTTCTTTGTAAGAAATCTGAATCTGTTCGGCAAGGTTAGCGTCATAACACGGTTGCGGCAGACGATCTTCGCAATCACACAACAACAATTCGTCAATAAAGCGCTCCAGTTTGTCATCCGCCTGCCAACGTAGCGGGCTGAACAATTCAAAATGCCGAAAAGTGCGGTCGGTGTTCGCTATGAATTTTAACAAGAAGCCCCTGCCCGTACCCTCGTAACTGTGAATGGTCGTGGTCAGCACAAtgc  <  1:429962/251‑3 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                CCAAATTACCACGTGGACGACGTTGTCCGCGCCGAATTTTACGAATTAATGCGTCGTCACCAAATTTGCCCTCAGGCACCGCCCACACACAACCCAACAAGGCCTCCTGCGTCTGCGCAAGATAAAATTGTTGTCGCGGCGCATCCAATAATCGACGTAAATCCAACGGCGAGGTTCGGTAATGCGCCAAGGTCAGCAAGCCATAAACCGATTCGATTTGATAAGGCGGAATCTGTTCTTCGTAAGAAATCTGAATCTGTTTGGCAAGGTTGGCATCGTAACGCGGTTGCGTCAGACGATCTTCGCAATCACACAACAACAATTCATCAATAAAGCGCTCCAGTTTGTCATCCGCCTGCCAACGTAACGGGCTGAACAATTCAAAATGCCGCAAAGTGCGGTCGGTTTTCGTCATGAATTTTAACAAGAAGCCCCTGCCCGTGCCTTCGTAGCTGTGAATGGTGGTCGTCAGCACAATAC  >  1/1474787‑1475266

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑