Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,474,907	G→A	L391F (CTT→TTT)	unknown ←	tRNA cytosine(34) acetyltransferase (EC 2.3.1.193)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,474,907	0	G	A	100.0%	139.4 / NA	53	L391F (CTT→TTT)	unknown	tRNA cytosine(34) acetyltransferase (EC 2.3.1.193)
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (31/22);  total (31/22)

CGATTAAACGTTGCCCCAGTCCCCGCTGCTGCCAGTTGGGTTGCACGGCGATACGGGAAATGCGCAGTGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTACCACGTGGACGACGTTGTCCGCGCCGAATTTTACGAATTAATGCGTCGTCACCAAATTTGCCCTCAGGCACCGCCCACACACAACCCAACAAGGCCTCCTGCGTCTGCGCAAGATAAAATTGTTGTCGCGGCGCATCCAATAATCGACGTAAATCCAACGGCGAGGTTCGGTAATGCGCCAAGGTCAGCAAGCCATAAACCGATTCGATTTGATAAGGCGGAATCTGTTCTTCGTAAGAAATCTGAATCTGTTTGGCAAGGTTGGCATCGTAACGCGGTTGCGTCAGACGATCTTCGCAATCACACAACAACAATTCATCAATAAAGCGCTCCAGTTTGTCA  >  1/1474664‑1475136                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                      cGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCaaaataaaat                                                                                                                                                                                                                                >  1:445071/1‑251 (MQ=255)   aTTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAAttg                                                                                                                                                                                                                              >  1:143521/1‑251 (MQ=255)              ccccAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTCgcggtg                                                                                                                                                                                                                   >  1:759887/1‑249 (MQ=255)                     cctcGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTCGCGGTGCATCCaa                                                                                                                                                                                                            <  1:781539/248‑1 (MQ=255)                      ctcGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTCGCGGTGCATCCaat                                                                                                                                                                                                           <  1:483325/249‑1 (MQ=255)                      ctcGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTCGCGGTGCATCCaat                                                                                                                                                                                                           <  1:232337/249‑1 (MQ=255)                         gctgctGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTCGCGGTGCATCCaataat                                                                                                                                                                                                        >  1:69320/1‑251 (MQ=255)                          ctgctgCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTCGCGGTGCATCCaa                                                                                                                                                                                                            <  1:789812/246‑1 (MQ=255)                                      gggctGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTCGCGGTGCATCCAATAATCGACGTAAATCCa                                                                                                                                                                                           >  1:158901/5‑251 (MQ=255)                                           gCACGGCGATCCGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGACACCGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCaaaataaaat                                                                                                                                                                                                                                <  1:615681/209‑1 (MQ=255)                                                        ggAAATGCGCAAAGGACCCAATTTCCACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCTCCGCCCACACTCAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTCTCGGTGCATCCAATAATCGTCGTAAATCCAACGGCGAGGtgctgtaa                                                                                                                                                                          <  1:210964/250‑8 (MQ=255)                                                        ggAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTCGCGGTGCATCCAATAATCGACGTAAATCCAACGGCGAGGTGCGGTaa                                                                                                                                                                          <  1:209876/250‑1 (MQ=255)                                                        ggAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTCGCGGTGCATCCAATAATCGACGTAAATCCAACGGCGAGGTGCGGTaa                                                                                                                                                                          <  1:51847/250‑1 (MQ=255)                                                        ggAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTCGCGGTGCATCCAATAATCGACGTAAATCCAACGGCGAGGTGCGGTaa                                                                                                                                                                          >  1:582402/1‑250 (MQ=255)                                                        ggAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTCGCGGTGCATCCAATAATCGACGTAAATCCAACGGCGAGGTGCGGTaa                                                                                                                                                                          <  1:698893/250‑1 (MQ=255)                                                                              ttACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTcgc                                                                                                                                                                                                                       >  1:283615/1‑183 (MQ=255)                                                                              ttACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTCGCGGTGCATCCAATAATCGACGTAAATCCAACGGCGAGGTGCGGt                                                                                                                                                                            <  1:632915/226‑1 (MQ=255)                                                                              ttACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTCGCGGTGCATCCAATAATCGACGTAAATCCAACGGCGAGGTGCGGTAATGCGCCAAAGTCAGCAAGCCATa                                                                                                                                                   >  1:733341/1‑251 (MQ=255)                                                                                   cGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGCCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTCGCGGTGCATCCAATAATCGACGTAAATCCAACGGCGAGGTGAGGTAATGCGCCAAAGTCAGCAAGCCATAAACcg                                                                                                                                              >  1:506287/1‑251 (MQ=255)                                                                                        gTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTCGCGGTGCATCCAATAATCGACGTAAATCCAACGGCGAGGTGCGGTaa                                                                                                                                                                          <  1:728742/218‑1 (MQ=255)                                                                                        gTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTCGCGGTGCATCCAATAATCGACGTAAATCCAACGGCGAGGTGCGGTAATGCGCCAAAGTCAGCAAGCCATAAAc                                                                                                                                                >  1:882176/1‑244 (MQ=255)                                                                                                            cacaACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTCGCGGTGCATCCAATAATCGACGTAAATCCAACGGCGAGGTGCGGTaa                                                                                                                                                                          >  1:701921/1‑198 (MQ=255)                                                                                                            cacaACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTCGCGGTGCATCCAATAATCGACGTAAATCCAACGGCGAGGTGCGGTAATGCGCCAAAGTCAGCAAGCCATAAACCGATTCGATTTGATTAGGCGGAATccg                                                                                                                     >  1:390128/1‑249 (MQ=255)                                                                                                              caacaTTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTCGCGGTGCATCCAATAATCGACGTAAATCCAACGGCGAGGTGCGGTAATGCGCCAAAGTCAGCAAGCCATAAACCGATTCGATTTGATTAGGCGGAATCCGtt                                                                                                                   >  1:809807/1‑251 (MQ=255)                                                                                                                 caTTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTCGCGGTGCATCCAATAATCGACGTAAATCCAACGGCGAGGTGCGGTAATGCGCCAAAGTCAGc                                                                                                                                                           <  1:390954/208‑1 (MQ=255)                                                                                                                 caTTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTCGCGGTGCATCCAATAATCGACGTAAATCCAACGGCGAGGTGCGGTAATGCGCCAAAGTCAGCAAGCCATAAACCGATTCGATTTGATTAGGCGGAATCCGttctt                                                                                                                >  1:798858/1‑251 (MQ=255)                                                                                                                            ccaaattgccgcgcaggcgacgTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTCGCGGTGCATCCAATAATCGACGTAAATCCAACGGCGAGGTGCGGTAATGCGCCAAAGTCAGCAAGCCATAAACCGATTCGATTTGATTAGGCGGAATCCGTTCTTTGTAAGAaat                                                                                                      >  1:130677/18‑250 (MQ=255)                                                                                                                            ccaaattgccgcgcaggcgacgTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTCGCGGTGCATCCAATAATCGACGTAAATCCAACGGCGAGGTGCGGTAATGCGCCAAAGTCAGCAAGCCATAAACCGATTCGATTTGATTAGGCGGAATCCGTTCTTTGTAAGAaat                                                                                                      >  1:28453/18‑250 (MQ=255)                                                                                                                                         caggcgacgTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTCGCGGTGCATCCAATAATCGACGTAAATCCAACGGCGAGGTGCGGTAATGCGCCAAAGTCAGCAAGCCATAAACcgatt                                                                                                                                           <  1:114989/196‑1 (MQ=255)                                                                                                                                              gacgTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTCGCGGTGCATCCAATAATCGACGTAAATCCAACGGCGAGGTGCGGTAATGCGCCAAAGTCAGCAAGCCATAAAcc                                                                                                                                               <  1:739850/191‑1 (MQ=255)                                                                                                                                                    gTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTCGCGGTGCATCCAATAATCGACGTAAATCCAACGGCGAGGTGCGGTaa                                                                                                                                                                          >  1:97440/1‑158 (MQ=255)                                                                                                                                                           tcGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTCGCGGTGCATCCAATAATCGACGTAAATCCAACGGCGAGGTGCGGTAATGCGCCAAAGTCAGCAAGCCATAAACCGATTCGATTTGATTAGGCGGAATCCGTTCTTTGTAAGAAATCTGAATCTGTTCGGCAAGGttagcgtcataac                                                                      <  1:177839/250‑12 (MQ=255)                                                                                                                                                             gAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTCGCGGTGCATCCAATAATCGACGTAAATCCAACGGCGAGGTGCGGTAATGCGCCAAAGTCAGCAAGCCATAAACCGATTCGATTTGATTAGGCGGAATCCGttctttg                                                                                                              <  1:803115/209‑3 (MQ=255)                                                                                                                                                              aaTTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTCGCGGTGCATCCAATAATCGACGTAAATCCAACGGCGAGGTGCGGTAATGCGCCAAAGTCAGCAAGCCATAAACCGATTCGATTTGATTAGGCGGAATCCGTTCTTTGTAAGAAATCTGAATCTGTTCGGCAAGGttagcgtcataacac                                                                    <  1:283929/250‑14 (MQ=255)                                                                                                                                                              aaTTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTCGCGGTGCATCCAATAAGCGACGTAAATCCAACGGCGAGGTGCGGTAATGCGCCAAAGTCAGCAAGCCATAAACCGATTCGATTTGATTAGGCGGAATCCGTTCTTTGTAAGAAATCTGAATCTGTTCGGCAAGGttagcgtcataacac                                                                    <  1:890573/250‑14 (MQ=255)                                                                                                                                                                                   atcctcaaatcctccttCAGGCACCGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTCGCGGTGCATCCAATAATCGACGTAAATCCAACGGCGAGGTGCGGTAATGCGCCAAAGTCAGCAAGCCATAAACCGATTCGATTTGATTAGGCGGAATCCGTTCTTTGTAAGAAATCTGAATCTGTTCGGCAAGGTTAGCGTCATAACACGGTTGCGGCAGACGATCTTCg                                               >  1:674652/17‑250 (MQ=255)                                                                                                                                                                                   atcctcaaatcctccttCAGGCACCGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTCGCGGTGCATCCAATAATCGACGTAAATCCAACGGCGAGGTGCGGTAATGCGCCAAAGTCAGCAAGCCATAAACCGATTCGATTTGATTAAGCGGAATCCGTTCTTTGTAAGAAATCTGAATCTGTTCGGCAAGGTTAGCGTCATAACACGGTTGCGGCAGACGATCTTCg                                               >  1:443662/17‑250 (MQ=255)                                                                                                                                                                                        caaatcctccttCAGGCACCGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTCGCGGTGCATCCAATAATCGACGTAAATCCAACGGCGAGGTGCGGTAATGCGCCAAAGTCAGCAAGCCATAAACCGATTCGATTTGATTAGGCGGAATCCGTTCTTTGTAAGAAATCTGAATCTGTTCGGCAAGGttagcgt                                                                            >  1:695858/12‑211 (MQ=255)                                                                                                                                                                                           atcctccttCAGGCACCGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTCGCGGTGCATCCAATAATCGACGTAAATCCAACGGCGAGGTGCGGTAATGCGCCAAAGTCAGCAAGCCATAAACCGATTCGATTTGATTAGGCGGAATCCGTTCTTTGTAAGAAATCTGAATCTGTTCGGCAAGGTTAGCGTCATAACACGGTTGCGGCAGACGATCTTCGCAATcacac                                      <  1:406502/243‑1 (MQ=255)                                                                                                                                                                                                ccttCAGGCACCGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTCGCGGTGCATCCAATAATCGACGTAAATCCAACGGCGAGGTGCGGTAATGCGCCAAAGTCAGCAAGCCATAAAc                                                                                                                                                >  1:229874/4‑140 (MQ=255)                                                                                                                                                                                                 cttCAGGCACCGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTCGCGGTGCATCCAATAATCGACGTAAATCCAACGGCGAGGTGCGGTAATGCGCCAAAGTCAGCAAGCCATAAACCGATTCGATTTGATTAGGCGGAATCCGTTCTTTGTAAGAAATCTGAATCTGTTCGGCAAGGTTAGCGTCATAACACGGTTGCGGCAGACGAt                                                    <  1:823891/229‑1 (MQ=255)                                                                                                                                                                                                 cttCAGGCACCGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTCGCGGTGCATCCAATAATCGACGTAAATCCAACGGCGAGGTGCGGTAATGCGCCAAAGTCAGCAAGCCATAAACCGATTCGATTTGATTAGGCGGAATCCGTTCTTTGTAAGAAATCTGAATCTGTTCGGCAAGGTTAGCGTCATAACACGGTTGCGGCAGACGATCTTCGCaa                                            <  1:342096/237‑1 (MQ=255)                                                                                                                                                                                                 cttCAGGCACCGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTCGCGGTGCATCCAATAATCGACGTAAATCCAACGGCGAGGTGCGGTAATGCGCCAAAGTCAGCAAGCCATAAACCGATTCGATTTGATTAGGCGGAATCCGTTCTTTGTAAGAAATCTGAATCTGTTCGGCAAGGTTAGCGTCATAACACGGTTGCGGCAGACGATCTTCGCAATCACacaacaa                                 >  1:619874/3‑250 (MQ=255)                                                                                                                                                                                                  ttCAGGCACCGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTCGCGGTGCATCCAATAATCGACGTAAATCCAACGGCGAGGTGCGGTAATGCGCCAAAGTCAGCAAGCCATAAACCGATTCGATTTGATTAGGCGGAATCCGTTCTTTGTAAGAAATCTGAATCTGTTCGGCAAGGttagcgtcataacac                                                                    >  1:252535/2‑201 (MQ=255)                                                                                                                                                                                                  ttCAGGCACCGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTCGCGGTGCATCCAATAATCGACGTAAATCCAACGGCGAGGTGCGGTAATGCGCCAAAGTCAGCAAGCCATAAACCGATTCGATTTGATTAGGCGGAATCCGTTCTTTGTAAGAAATCTGAATCTGTTCGGCAAGGTTAGCGTCATAACACGGTTGCGGCAGACGATCTTCGCAATCACacaacaaca                               >  1:175357/2‑251 (MQ=255)                                                                                                                                                                                                          ccGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTCGCGGTGCATCCAATAATCGACGTAAATCCAACGGCGAGGTGCGGTAATGCGCCAAAGTCAGCaa                                                                                                                                                         >  1:685081/1‑121 (MQ=255)                                                                                                                                                                                                          ccGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTCGCGGTGCATCCAATAATCGACGTAAATCCAACGGCGAGGTGCGGTAATGCGCCAAAGTCAGCAAGCCATAAACCGATTCGATTTGATTAGGCGGAATCCGTTCTTTGTAAGAAATCTGAATCTGTTCGGCAAGGTTAGCGTCATAACACGGTTGCGGCAGACGATCTTCGCAATCACACAACAACAATtcgtca                       >  1:607949/1‑251 (MQ=255)                                                                                                                                                                                                             cccACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTCGCGGTGCATCCAATAATCGACGTAAATCCAACGGCGAGGTGCGGTAATGCGCCAAAGTCAGCAAGCCATAAACCGATTCGATTTGATTAGGCGGAATCCGTTCTTTGTAAGAAATCTGAATCTGTTCGGCAAGGTTAGCGTCATAACACGGTTGCGGCAGACGATCTTCGCAATCACACAACAACAATTCGTCAATa                    >  1:278758/1‑251 (MQ=255)                                                                                                                                                                                                                acacacaGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTCGCGGTGCATCCAATAATCGACGTAAATCCAACGGCGAGGTGCGGTAATGCGCCAAAGTCAGCAAGCCATAAACCGATTCGATTTGATTAGGCGGAATCCGTTCTTTGTAAGAAATCTGAATCTGTTCGGCAAGGTTAGCGTCATAACACGGTTGCGGCAGACGATCTTCGCAATCACACAACAACAATTCGTCAATAAAg                 >  1:39237/1‑251 (MQ=255)                                                                                                                                                                                                                 cacacaGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTCGCGGTGCATCCAATAATCGACGTAAATCCAACGGCGAGGTGCGGTAATGCGCCAAAGTCAGCAAGCCATAAACCGATTCGATTTGATTAGGCGGAATCCGTTCTTTGTAAGAAATCTGAATCTGTTCGGCAAGGttagcgt                                                                            <  1:290264/191‑6 (MQ=255)                                                                                                                                                                                                                 cacacaGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTCGCGGTGCATCCAATAATCGACGTAAATCCAACGGCGAGGTGCGGTAATGCGCCAAAGTCAGCAAGCCATAAACCGATTCGATTTGATTAGGCGGAATCCGTTCTTTGTAAGAAATCTGAATCTGTTCGGCAAGGTTAGCGTCATAACACGGTTGCGGCAGACGATCTTCGCAATCACACAACAACAATTCGTCAATAAAg                 >  1:420895/1‑250 (MQ=255)                                                                                                                                                                                                                       gcccaacagggtttcccgcgtttgtgcAAAATAAAATTGTTGTCGCGGTGCATCCAATAATCGACGTAAATCCAACGGCGAGGTGCGGTAATGCGCCAAAGTCAGCAAGCCATAAACCGATTCGATTTGATTAGGCGGAATCCGTTCTTTGTAAGAAATCTGAATCTGTTCGGCAAGGTTAGCGTCATAACACGGTTGCGGCAGACGATCTTCGCAATCACACAACAACAATTCGTCAATAAAGCGCTCCa          >  1:367894/26‑251 (MQ=255)                                                                                                                                                                                                                       gcccaacagggtttcccgcgtttgtgcAAAATAAAATTGTTGTCGCGGTGCATCCAATAATCGACGTAAATCCAACGGCGAGGTGCGGTAATGCGCCAAAGTCAGCAAGCCATAAACCGATTCGATTTGATTAGGCGGAATCCGTTCTTTGTAAGAAATCTGAATCTGTTCGGCAAGGTTAGCGTCATAACACGGTTGCGGCAGACGATCTTCGCAATCACACAACAACAATTCGTCAATAAAGCGCTCCa          >  1:527982/26‑251 (MQ=255)                                                                                                                                                                                                                          caacagggtttcccgcgtttgtgcAAAATAAAATTGTTGTCGCGGTGCATCCAATAATCGACGTAAATCCAACGGCGAGGTGCGGTAATGCGCCAAAGTCAGCAAGCCATAAACCGATTCGATTTGATTAGGCGGAATCCGTTCTTTGTAAGAAATCTGAATCTGTTCGGCAAGGTTAGCGTCATAACACGGTTGCGGCAGACGATCTTCGCAATCACACAACAACAATTCGTCAATAAAGCGCTCCAGtt       >  1:604455/23‑251 (MQ=255)                                                                                                                                                                                                                                ggtttcccgcgtttgtgcAAAATAAAATTGTTGTCGCGGTGCATCCAATAATCGACGTAAATCCAACGGCGAGGTGCGGTAATGCGCCAAAGTCAGCAAGCCATAAACCGATTCGATTTGATTAGGCGGAATCCGTTCTTTGTAAGAAATCTGAATCTGTTCGGCAAGGTTAGCGTCATAACACGGTTGCGGCAGACGATCTTCGCAATCACACAACAACAATTCGTCAATAAAGCGCTCCAGTTTGTCa  <  1:428935/234‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                      CGATTAAACGTTGCCCCAGTCCCCGCTGCTGCCAGTTGGGTTGCACGGCGATACGGGAAATGCGCAGTGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTACCACGTGGACGACGTTGTCCGCGCCGAATTTTACGAATTAATGCGTCGTCACCAAATTTGCCCTCAGGCACCGCCCACACACAACCCAACAAGGCCTCCTGCGTCTGCGCAAGATAAAATTGTTGTCGCGGCGCATCCAATAATCGACGTAAATCCAACGGCGAGGTTCGGTAATGCGCCAAGGTCAGCAAGCCATAAACCGATTCGATTTGATAAGGCGGAATCTGTTCTTCGTAAGAAATCTGAATCTGTTTGGCAAGGTTGGCATCGTAACGCGGTTGCGTCAGACGATCTTCGCAATCACACAACAACAATTCATCAATAAAGCGCTCCAGTTTGTCA  >  1/1474664‑1475136

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑