Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,474,839	G→A	D413D (GAC→GAT)	unknown ←	tRNA cytosine(34) acetyltransferase (EC 2.3.1.193)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,474,839	0	G	A	100.0%	151.2 / NA	56	D413D (GAC→GAT)	unknown	tRNA cytosine(34) acetyltransferase (EC 2.3.1.193)
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (24/32);  total (24/32)

ACGGAAAGGAAATCCACCGCACGTTGTTGTTTAACTC‑GTTCGATTAAACGTTGCCCCAGTCCCCGCTGCTGCCAGTTGGGTTGCACGGCGATACGGGAAATGCGCAGTGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTACCACGTGGACGACGTTGTCCGCGCCGAATTTTACGAATTAATGCGTCGTCACCAAATTTGCCCTCAGGCACCGCCCACACACAACCCAACAAGGCCTCCTGCGTCTGCGCAAGATAAAATTGTTGTCGCGGCGCATCCAATAATCGACGTAAATCCAACGGCGAGGTTCGGTAATGCGCCAAGGTCAGCAAGCCATAAACCGATTCGATTTGATAAGGCGGAATCTGTTCTTCGTAAGAAATCTGAATCTGTTTGGCAAGGTTGGCATCGTAACGC  >  1/1474624‑1475070                                                                                                                                                                                                                         |                                                                                                                                                                                                                                        aCGGAAAGAAAGTCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCGGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATGCGGGAAATGCGCAAAGATCGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCcaca                                                                                                                                                                                                       <  1:85052/250‑1 (MQ=255) aCGGAAAGAAAATCCACCGCAGGTTGTTGTTTAA‑TCGGTTCGATTAAACGTGGCCCCAGCCCTCGCTGCGGCCAGTTGGGCTGCACGGCGATACGGAAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCcacac                                                                                                                                                                                                      <  1:16077/251‑1 (MQ=255) aCGGAAAGAAAATCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCcacac                                                                                                                                                                                                      <  1:598662/251‑1 (MQ=255)    gaaagaaaaTCCCCCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCcacacac                                                                                                                                                                                                    <  1:30271/250‑1 (MQ=255)    gaaagaaaaTCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCcacacac                                                                                                                                                                                                    <  1:892622/250‑1 (MQ=255)           aaTCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCcaac                                                                                                                                                                                            <  1:386119/251‑1 (MQ=255)             cccACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCcaacag                                                                                                                                                                                          <  1:596187/250‑2 (MQ=255)             cccACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCcaacag                                                                                                                                                                                          <  1:308059/250‑2 (MQ=255)                     aCGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCcacacac                                                                                                                                                                                                    <  1:297852/233‑1 (MQ=255)                     aCGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCcaacagggtttcc                                                                                                                                                                                   >  1:91252/1‑242 (MQ=255)                      cGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCcacac                                                                                                                                                                                                      <  1:323252/230‑1 (MQ=255)                        ttgttgttTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCcacacac                                                                                                                                                                                                    >  1:471552/1‑230 (MQ=255)                        ttgttgttTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCcacac                                                                                                                                                                                                      >  1:437368/1‑228 (MQ=255)                        ttgttgttTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCcaacagggtttcccgcg                                                                                                                                                                               >  1:585054/1‑239 (MQ=255)                          gttgttTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCcaacagggtttcccgcgtt                                                                                                                                                                             >  1:144890/1‑237 (MQ=255)                              gtttaatctgTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCcacacac                                                                                                                                                                                                    >  1:539036/10‑225 (MQ=255)                                          cGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCaaaataaaat                                                                                                                                                              >  1:445071/1‑251 (MQ=255)                                            aTTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTTCACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCcaacagggtttcccgcgtttgtgcaaaat                                                                                                                                                                   >  1:9417/1‑220 (MQ=255)                                            aTTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCcaacagggtttcccgcg                                                                                                                                                                               <  1:790218/232‑13 (MQ=255)                                            aTTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAAttg                                                                                                                                                            >  1:143521/1‑251 (MQ=255)                                                       ccccAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTCgcggtg                                                                                                                                                 >  1:759887/1‑249 (MQ=255)                                                              cctcGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTCGCGGTGCATCCaa                                                                                                                                          <  1:781539/248‑1 (MQ=255)                                                               ctcGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTCGCGGTGCATCCaat                                                                                                                                         <  1:232337/249‑1 (MQ=255)                                                               ctcGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTCGCGGTGCATCCaat                                                                                                                                         <  1:483325/249‑1 (MQ=255)                                                                  gctgctGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTCGCGGTGCATCCaataat                                                                                                                                      >  1:69320/1‑251 (MQ=255)                                                                   ctgctgCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTCGCGGTGCATCCaa                                                                                                                                          <  1:789812/246‑1 (MQ=255)                                                                               gggctGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTCGCGGTGCATCCAATAATCGACGTAAATCCa                                                                                                                         >  1:158901/5‑251 (MQ=255)                                                                                    gCACGGCGATCCGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGACACCGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCaaaataaaat                                                                                                                                                              <  1:615681/209‑1 (MQ=255)                                                                                         gCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCcac                                                                                                                                                                                                        <  1:830742/162‑1 (MQ=255)                                                                                             tACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCcacacac                                                                                                                                                                                                    <  1:833883/162‑1 (MQ=255)                                                                                                 ggAAATGCGCAAAGGACCCAATTTCCACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCTCCGCCCACACTCAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTCTCGGTGCATCCAATAATCGTCGTAAATCCAACGGCGAGGtgctgtaa                                                                                                        <  1:210964/250‑8 (MQ=255)                                                                                                 ggAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTCGCGGTGCATCCAATAATCGACGTAAATCCAACGGCGAGGTGCGGTaa                                                                                                        <  1:51847/250‑1 (MQ=255)                                                                                                 ggAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTCGCGGTGCATCCAATAATCGACGTAAATCCAACGGCGAGGTGCGGTaa                                                                                                        <  1:209876/250‑1 (MQ=255)                                                                                                 ggAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTCGCGGTGCATCCAATAATCGACGTAAATCCAACGGCGAGGTGCGGTaa                                                                                                        <  1:698893/250‑1 (MQ=255)                                                                                                 ggAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTCGCGGTGCATCCAATAATCGACGTAAATCCAACGGCGAGGTGCGGTaa                                                                                                        >  1:582402/1‑250 (MQ=255)                                                                                                                       ttACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTcgc                                                                                                                                                     >  1:283615/1‑183 (MQ=255)                                                                                                                       ttACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTCGCGGTGCATCCAATAATCGACGTAAATCCAACGGCGAGGTGCGGt                                                                                                          <  1:632915/226‑1 (MQ=255)                                                                                                                       ttACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTCGCGGTGCATCCAATAATCGACGTAAATCCAACGGCGAGGTGCGGTAATGCGCCAAAGTCAGCAAGCCATa                                                                                 >  1:733341/1‑251 (MQ=255)                                                                                                                            cGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGCCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTCGCGGTGCATCCAATAATCGACGTAAATCCAACGGCGAGGTGAGGTAATGCGCCAAAGTCAGCAAGCCATAAACcg                                                                            >  1:506287/1‑251 (MQ=255)                                                                                                                                 gTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCcaacag                                                                                                                                                                                          <  1:701442/136‑2 (MQ=255)                                                                                                                                 gTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTCGCGGTGCATCCAATAATCGACGTAAATCCAACGGCGAGGTGCGGTaa                                                                                                        <  1:728742/218‑1 (MQ=255)                                                                                                                                 gTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTCGCGGTGCATCCAATAATCGACGTAAATCCAACGGCGAGGTGCGGTAATGCGCCAAAGTCAGCAAGCCATAAAc                                                                              >  1:882176/1‑244 (MQ=255)                                                                                                                                                     cacaACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTCGCGGTGCATCCAATAATCGACGTAAATCCAACGGCGAGGTGCGGTaa                                                                                                        >  1:701921/1‑198 (MQ=255)                                                                                                                                                     cacaACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTCGCGGTGCATCCAATAATCGACGTAAATCCAACGGCGAGGTGCGGTAATGCGCCAAAGTCAGCAAGCCATAAACCGATTCGATTTGATTAGGCGGAATccg                                                   >  1:390128/1‑249 (MQ=255)                                                                                                                                                       caacaTTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTCGCGGTGCATCCAATAATCGACGTAAATCCAACGGCGAGGTGCGGTAATGCGCCAAAGTCAGCAAGCCATAAACCGATTCGATTTGATTAGGCGGAATCCGtt                                                 >  1:809807/1‑251 (MQ=255)                                                                                                                                                          caTTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTCGCGGTGCATCCAATAATCGACGTAAATCCAACGGCGAGGTGCGGTAATGCGCCAAAGTCAGc                                                                                         <  1:390954/208‑1 (MQ=255)                                                                                                                                                          caTTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTCGCGGTGCATCCAATAATCGACGTAAATCCAACGGCGAGGTGCGGTAATGCGCCAAAGTCAGCAAGCCATAAACCGATTCGATTTGATTAGGCGGAATCCGttctt                                              >  1:798858/1‑251 (MQ=255)                                                                                                                                                                     ccaaattgccgcgcaggcgacgTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTCGCGGTGCATCCAATAATCGACGTAAATCCAACGGCGAGGTGCGGTAATGCGCCAAAGTCAGCAAGCCATAAACCGATTCGATTTGATTAGGCGGAATCCGTTCTTTGTAAGAaat                                    >  1:130677/18‑250 (MQ=255)                                                                                                                                                                     ccaaattgccgcgcaggcgacgTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTCGCGGTGCATCCAATAATCGACGTAAATCCAACGGCGAGGTGCGGTAATGCGCCAAAGTCAGCAAGCCATAAACCGATTCGATTTGATTAGGCGGAATCCGTTCTTTGTAAGAaat                                    >  1:28453/18‑250 (MQ=255)                                                                                                                                                                                  caggcgacgTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTCGCGGTGCATCCAATAATCGACGTAAATCCAACGGCGAGGTGCGGTAATGCGCCAAAGTCAGCAAGCCATAAACcgatt                                                                         <  1:114989/196‑1 (MQ=255)                                                                                                                                                                                       gacgTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTCGCGGTGCATCCAATAATCGACGTAAATCCAACGGCGAGGTGCGGTAATGCGCCAAAGTCAGCAAGCCATAAAcc                                                                             <  1:739850/191‑1 (MQ=255)                                                                                                                                                                                             gTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTCGCGGTGCATCCAATAATCGACGTAAATCCAACGGCGAGGTGCGGTaa                                                                                                        >  1:97440/1‑158 (MQ=255)                                                                                                                                                                                                    tcGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTCGCGGTGCATCCAATAATCGACGTAAATCCAACGGCGAGGTGCGGTAATGCGCCAAAGTCAGCAAGCCATAAACCGATTCGATTTGATTAGGCGGAATCCGTTCTTTGTAAGAAATCTGAATCTGTTCGGCAAGGttagcgtcataac    <  1:177839/250‑12 (MQ=255)                                                                                                                                                                                                      gAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTCGCGGTGCATCCAATAATCGACGTAAATCCAACGGCGAGGTGCGGTAATGCGCCAAAGTCAGCAAGCCATAAACCGATTCGATTTGATTAGGCGGAATCCGttctttg                                            <  1:803115/209‑3 (MQ=255)                                                                                                                                                                                                       aaTTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTCGCGGTGCATCCAATAATCGACGTAAATCCAACGGCGAGGTGCGGTAATGCGCCAAAGTCAGCAAGCCATAAACCGATTCGATTTGATTAGGCGGAATCCGTTCTTTGTAAGAAATCTGAATCTGTTCGGCAAGGttagcgtcataacac  <  1:283929/250‑14 (MQ=255)                                                                                                                                                                                                       aaTTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTCGCGGTGCATCCAATAAGCGACGTAAATCCAACGGCGAGGTGCGGTAATGCGCCAAAGTCAGCAAGCCATAAACCGATTCGATTTGATTAGGCGGAATCCGTTCTTTGTAAGAAATCTGAATCTGTTCGGCAAGGttagcgtcataacac  <  1:890573/250‑14 (MQ=255)                                                                                                                                                                                                                         |                                                                                                                                                                                                                                        ACGGAAAGGAAATCCACCGCACGTTGTTGTTTAACTC‑GTTCGATTAAACGTTGCCCCAGTCCCCGCTGCTGCCAGTTGGGTTGCACGGCGATACGGGAAATGCGCAGTGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTACCACGTGGACGACGTTGTCCGCGCCGAATTTTACGAATTAATGCGTCGTCACCAAATTTGCCCTCAGGCACCGCCCACACACAACCCAACAAGGCCTCCTGCGTCTGCGCAAGATAAAATTGTTGTCGCGGCGCATCCAATAATCGACGTAAATCCAACGGCGAGGTTCGGTAATGCGCCAAGGTCAGCAAGCCATAAACCGATTCGATTTGATAAGGCGGAATCTGTTCTTCGTAAGAAATCTGAATCTGTTTGGCAAGGTTGGCATCGTAACGC  >  1/1474624‑1475070

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑