Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,474,803	A→G	R425R (CGT→CGC)	unknown ←	tRNA cytosine(34) acetyltransferase (EC 2.3.1.193)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,474,803	0	A	G	100.0%	183.3 / NA	68	R425R (CGT→CGC)	unknown	tRNA cytosine(34) acetyltransferase (EC 2.3.1.193)
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (35/33);  total (35/33)

ACCAAAATAAAACCGCACTTTTGCCAAAACGCCAGCAGCTCGGGGGTGTAACCGAAACTGACGGAAAGGAAATCCACCGCACGTTGTTGTTTAACTC‑GTTCGATTAAACGTTGCCCCAGTCCCCGCTGCTGCCAGTTGGGTTGCACGGCGATACGGGAAATGCGCAGTGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTACCACGTGGACGACGTTGTCCGCGCCGAATTTTACGAATTAATGCGTCGTCACCAAATTTGCCCTCAGGCACCGCCCACACACAACCCAACAAGGCCTCCTGCGTCTGCGCAAGATAAAATTGTTGTCGCGGCGCATCCAATAATCGACGTAAATCCAACGGCGAGGTTCGGTAATGCGCCAAGGTCAGCAAGCCATAAACCGATTCGATTTGATAAGGCGGAATCTGTTCTT  >  1/1474564‑1475026                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                cccAAAATAAAACCGCACTTTTGCCAAAACGCGAGCAGTTCGGGGGTGTAACCGAAACTGACGGAAAGAAAATCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTcc                                                                                                                                                                                                                      <  1:101494/250‑1 (MQ=255)     aaaTAAAACCGCACTTTTGCCAAAACGCGAGCAGTTCGGGGGTGTAACCGAAACTGACGGAAAGAAAATCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCcgcgt                                                                                                                                                                                                                  >  1:135587/1‑250 (MQ=255)                                    cagttCGGGGGTGTAACCGAAACTGACGGAAAGAAAATCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGcatcatcctcaa                                                                                                                                                                                   >  1:305880/5‑240 (MQ=255)                                      gttCGGGGGTGTAACCGAAACTGACGGAAAGAAAATCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAACCACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGcatcatcctcaaat                                                                                                                                                                                 >  1:446276/3‑238 (MQ=255)                                      gttCGGGGGTGTAACCGAAACTGACGGAAAGAAAATCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAAtttgac                                                                                                                                                                                                        >  1:143746/3‑225 (MQ=255)                                      gttCGGGGGTGTAACCGAAACTGACGGAAAGAAAATCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGcatcatcctcaaat                                                                                                                                                                                 >  1:149617/3‑238 (MQ=255)                                      gttCGGGGGTGTAACCGAAACTGACGGAAAGAAAATCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGcatcatcctcaaat                                                                                                                                                                                 >  1:836462/3‑238 (MQ=255)                                      gttCGGGGGTGTAACCGAAACTGACGGAAAGAAAATCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGcatcatcctcaaat                                                                                                                                                                                 >  1:217141/3‑238 (MQ=255)                                      gttCGGGGGTGTAACCGAAACTGACGGAAAGAAAATCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGcatcatcctcaaat                                                                                                                                                                                 >  1:543915/3‑238 (MQ=255)                                      gttCGGGGGTGTAACCGAAACTGACGGAAAGAAAATCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGcatcatcctcaaat                                                                                                                                                                                 >  1:578174/3‑238 (MQ=255)                                      gttCGGGGGTGTAACCGAAACTGACGGAAAGAAAATCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGcat                                                                                                                                                                                            <  1:366912/238‑3 (MQ=255)                                       ttCGGGGGTGTAACCGAAACTGACGGAAAGAAAATCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAACCGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGcatcatcctcaaatc                                                                                                                                                                                <  1:770770/250‑15 (MQ=255)                                           ggggTGTAACCGAAACTGACGGAAAGAAAATCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGcat                                                                                                                                                                                            >  1:848980/1‑233 (MQ=255)                                               tgtAACCGAAACTGACGGAAAGAAAATCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGcatcatcctcaaatcctccttc                                                                                                                                                                         <  1:338311/250‑22 (MQ=255)                                                             aCGGAAAGAAAGTCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCGGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATGCGGGAAATGCGCAAAGATCGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCcaca                                                                                                                                                           <  1:85052/250‑1 (MQ=255)                                                             aCGGAAAGAAAATCCACCGCAGGTTGTTGTTTAA‑TCGGTTCGATTAAACGTGGCCCCAGCCCTCGCTGCGGCCAGTTGGGCTGCACGGCGATACGGAAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCcacac                                                                                                                                                          <  1:16077/251‑1 (MQ=255)                                                             aCGGAAAGAAAATCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCcacac                                                                                                                                                          <  1:598662/251‑1 (MQ=255)                                                              cGGAAAGAAAATCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGcatca                                                                                                                                                                                          >  1:390200/1‑214 (MQ=255)                                                                gaaagaaaaTCCCCCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCcacacac                                                                                                                                                        <  1:30271/250‑1 (MQ=255)                                                                gaaagaaaaTCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCcacacac                                                                                                                                                        <  1:892622/250‑1 (MQ=255)                                                                       aaTCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCcaac                                                                                                                                                <  1:386119/251‑1 (MQ=255)                                                                         cccACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCcaacag                                                                                                                                              <  1:596187/250‑2 (MQ=255)                                                                         cccACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCcaacag                                                                                                                                              <  1:308059/250‑2 (MQ=255)                                                                                 aCGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCcacacac                                                                                                                                                        <  1:297852/233‑1 (MQ=255)                                                                                 aCGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCcaacagggtttcc                                                                                                                                       >  1:91252/1‑242 (MQ=255)                                                                                  cGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCcacac                                                                                                                                                          <  1:323252/230‑1 (MQ=255)                                                                                    ttgttgttTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTcc                                                                                                                                                                                                                      <  1:489061/168‑1 (MQ=255)                                                                                    ttgttgttTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCcacacac                                                                                                                                                        >  1:471552/1‑230 (MQ=255)                                                                                    ttgttgttTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCcacac                                                                                                                                                          >  1:437368/1‑228 (MQ=255)                                                                                    ttgttgttTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCcaacagggtttcccgcg                                                                                                                                   >  1:585054/1‑239 (MQ=255)                                                                                      gttgttTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCcaacagggtttcccgcgtt                                                                                                                                 >  1:144890/1‑237 (MQ=255)                                                                                         tgtttaatctgTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGcat                                                                                                                                                                                            <  1:325522/180‑3 (MQ=255)                                                                                          gtttaatctgTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGcatcatcc                                                                                                                                                                                       <  1:487161/185‑8 (MQ=255)                                                                                          gtttaatctgTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCcacacac                                                                                                                                                        >  1:539036/10‑225 (MQ=255)                                                                                                      cGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCaaaataaaat                                                                                                                  >  1:445071/1‑251 (MQ=255)                                                                                                        aTTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTTCACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCcaacagggtttcccgcgtttgtgcaaaat                                                                                                                       >  1:9417/1‑220 (MQ=255)                                                                                                        aTTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCcaacagggtttcccgcg                                                                                                                                   <  1:790218/232‑13 (MQ=255)                                                                                                        aTTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAAttg                                                                                                                >  1:143521/1‑251 (MQ=255)                                                                                                                   ccccAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTCgcggtg                                                                                                     >  1:759887/1‑249 (MQ=255)                                                                                                                        gccctcGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGcatca                                                                                                                                                                                          >  1:227295/6‑157 (MQ=255)                                                                                                                          cctcGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTCGCGGTGCATCCaa                                                                                              <  1:781539/248‑1 (MQ=255)                                                                                                                           ctcGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTCGCGGTGCATCCaat                                                                                             <  1:483325/249‑1 (MQ=255)                                                                                                                           ctcGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTCGCGGTGCATCCaat                                                                                             <  1:232337/249‑1 (MQ=255)                                                                                                                              gctgctGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTCGCGGTGCATCCaataat                                                                                          >  1:69320/1‑251 (MQ=255)                                                                                                                               ctgctgCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTCGCGGTGCATCCaa                                                                                              <  1:789812/246‑1 (MQ=255)                                                                                                                                           gggctGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTCGCGGTGCATCCAATAATCGACGTAAATCCa                                                                             >  1:158901/5‑251 (MQ=255)                                                                                                                                                gCACGGCGATCCGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGACACCGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCaaaataaaat                                                                                                                  <  1:615681/209‑1 (MQ=255)                                                                                                                                                gCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGcatcatcctcaaat                                                                                                                                                                                 >  1:147719/1‑133 (MQ=255)                                                                                                                                                     gCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCcac                                                                                                                                                            <  1:830742/162‑1 (MQ=255)                                                                                                                                                         tACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCcacacac                                                                                                                                                        <  1:833883/162‑1 (MQ=255)                                                                                                                                                             ggAAATGCGCAAAGGACCCAATTTCCACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCTCCGCCCACACTCAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTCTCGGTGCATCCAATAATCGTCGTAAATCCAACGGCGAGGtgctgtaa                                                            <  1:210964/250‑8 (MQ=255)                                                                                                                                                             ggAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTCGCGGTGCATCCAATAATCGACGTAAATCCAACGGCGAGGTGCGGTaa                                                            <  1:51847/250‑1 (MQ=255)                                                                                                                                                             ggAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTCGCGGTGCATCCAATAATCGACGTAAATCCAACGGCGAGGTGCGGTaa                                                            <  1:209876/250‑1 (MQ=255)                                                                                                                                                             ggAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTCGCGGTGCATCCAATAATCGACGTAAATCCAACGGCGAGGTGCGGTaa                                                            <  1:698893/250‑1 (MQ=255)                                                                                                                                                             ggAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTCGCGGTGCATCCAATAATCGACGTAAATCCAACGGCGAGGTGCGGTaa                                                            >  1:582402/1‑250 (MQ=255)                                                                                                                                                                                 aTTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGcat                                                                                                                                                                                            >  1:473155/1‑100 (MQ=255)                                                                                                                                                                                   ttACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTcgc                                                                                                         >  1:283615/1‑183 (MQ=255)                                                                                                                                                                                   ttACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTCGCGGTGCATCCAATAATCGACGTAAATCCAACGGCGAGGTGCGGt                                                              <  1:632915/226‑1 (MQ=255)                                                                                                                                                                                   ttACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTCGCGGTGCATCCAATAATCGACGTAAATCCAACGGCGAGGTGCGGTAATGCGCCAAAGTCAGCAAGCCATa                                     >  1:733341/1‑251 (MQ=255)                                                                                                                                                                                        cGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGCCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTCGCGGTGCATCCAATAATCGACGTAAATCCAACGGCGAGGTGAGGTAATGCGCCAAAGTCAGCAAGCCATAAACcg                                >  1:506287/1‑251 (MQ=255)                                                                                                                                                                                             gTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCcaacag                                                                                                                                              <  1:701442/136‑2 (MQ=255)                                                                                                                                                                                             gTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTCGCGGTGCATCCAATAATCGACGTAAATCCAACGGCGAGGTGCGGTaa                                                            <  1:728742/218‑1 (MQ=255)                                                                                                                                                                                             gTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTCGCGGTGCATCCAATAATCGACGTAAATCCAACGGCGAGGTGCGGTAATGCGCCAAAGTCAGCAAGCCATAAAc                                  >  1:882176/1‑244 (MQ=255)                                                                                                                                                                                                                 cacaACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTCGCGGTGCATCCAATAATCGACGTAAATCCAACGGCGAGGTGCGGTaa                                                            >  1:701921/1‑198 (MQ=255)                                                                                                                                                                                                                 cacaACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTCGCGGTGCATCCAATAATCGACGTAAATCCAACGGCGAGGTGCGGTAATGCGCCAAAGTCAGCAAGCCATAAACCGATTCGATTTGATTAGGCGGAATccg       >  1:390128/1‑249 (MQ=255)                                                                                                                                                                                                                   caacaTTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTCGCGGTGCATCCAATAATCGACGTAAATCCAACGGCGAGGTGCGGTAATGCGCCAAAGTCAGCAAGCCATAAACCGATTCGATTTGATTAGGCGGAATCCGtt     >  1:809807/1‑251 (MQ=255)                                                                                                                                                                                                                      caTTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTCGCGGTGCATCCAATAATCGACGTAAATCCAACGGCGAGGTGCGGTAATGCGCCAAAGTCAGc                                             <  1:390954/208‑1 (MQ=255)                                                                                                                                                                                                                      caTTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTCGCGGTGCATCCAATAATCGACGTAAATCCAACGGCGAGGTGCGGTAATGCGCCAAAGTCAGCAAGCCATAAACCGATTCGATTTGATTAGGCGGAATCCGttctt  >  1:798858/1‑251 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                ACCAAAATAAAACCGCACTTTTGCCAAAACGCCAGCAGCTCGGGGGTGTAACCGAAACTGACGGAAAGGAAATCCACCGCACGTTGTTGTTTAACTC‑GTTCGATTAAACGTTGCCCCAGTCCCCGCTGCTGCCAGTTGGGTTGCACGGCGATACGGGAAATGCGCAGTGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTACCACGTGGACGACGTTGTCCGCGCCGAATTTTACGAATTAATGCGTCGTCACCAAATTTGCCCTCAGGCACCGCCCACACACAACCCAACAAGGCCTCCTGCGTCTGCGCAAGATAAAATTGTTGTCGCGGCGCATCCAATAATCGACGTAAATCCAACGGCGAGGTTCGGTAATGCGCCAAGGTCAGCAAGCCATAAACCGATTCGATTTGATAAGGCGGAATCTGTTCTT  >  1/1474564‑1475026

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑