Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,474,704	T→C	Q458Q (CAA→CAG)	unknown ←	tRNA cytosine(34) acetyltransferase (EC 2.3.1.193)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,474,704	0	T	C	100.0%	176.7 / NA	63	Q458Q (CAA→CAG)	unknown	tRNA cytosine(34) acetyltransferase (EC 2.3.1.193)
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (28/35);  total (28/35)

TGCTCCGCCTGTTGCACCAACGCCCGTCCGCGCTCCGTCAGCGGGCACAATGCCACTACGGAATAACAGCCGCTGCCTGCATCTTTGCCTTCGCTGAAATGCACCAAAATAAAACCGCACTTTTGCCAAAACGCCAGCAGCTCGGGGGTGTAACCGAAACTGACGGAAAGGAAATCCACCGCACGTTGTTGTTTAACTC‑GTTCGATTAAACGTTGCCCCAGTCCCCGCTGCTGCCAGTTGGGTTGCACGGCGATACGGGAAATGCGCAGTGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTACCACGTGGACGACGTTGTCCGCGCCGAATTTTACGAATTAATGCGTCGTCACCAAATTTGCCCTCAGGCACCGCCCACACACAACCCAACAAGGCCTCCTGCGTCTGCGCAAGATAAAATTGTTGTCGCGGCGCATCCAATAAT  >  1/1474462‑1474938                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                           tgttCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCACCACGGAATAACAGCCGCTGCCCGCATCTTTGCTTTCGCTGAAATGCACCAAAATAAAACCGCACTTTTGCCAAAACGCGAGCAGTTCGGGGGTGTAACCGAAACTGACGGAAAGAAAATCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCTAGTTGGGCTGCACGGc                                                                                                                                                                                                                                    <  1:562036/248‑1 (MQ=255) tgttCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCACCACGGAATAACAGCCGCTGCCCGCATCTTTGCTTTCGCTGAAATGCACCAAAATAAAACCGCACTTTTGCCAAAACGCGAGCAGTTCGGGGGTGTAACCGAAACTGACGGAAAGAAAATCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGc                                                                                                                                                                                                                                    <  1:602337/248‑1 (MQ=255)                     cgcccgccCGTGCTCCGTTAGCGGACACAACGCCACCACGGAATAACAGCCGCTGCCCGCATCTTTGCTTTCGCTGAAATGCACCAAAATAAAACCGCACTTTTGCCAAAACGCGAGCAGTTCGGGGGTGTAACCGAAACTGACGGAAAGAAAATCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCaaag                                                                                                                                                                                                                <  1:289964/251‑4 (MQ=255)                       cccgcccgTGCTCCGTTAGCGGACACAACGCCACCACGGAATAACAGCCGCTGCCCGCATCTTTGCTTTCGCTGAAATGCACCAAAATAAAACCGCACTTTTGCCAAAACGCGAGCAGTTCGGGGGTGTAACCGAAACTGACGGAAAGAAAATCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCaaaga                                                                                                                                                                                                               <  1:369461/250‑5 (MQ=255)                                      ttaGCGGACACAACGCCACCACGGAATAACAGCCGCTGCCCGCATCTTTGCTTTCGCTGAAATGCACCAAAATAAAACCGCACTTTTGCCAAAACGCGAGCAGTTCGGGGGTGTAACCGAAACTGACGGAAAGAAAATCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGcc                                                                                                                                                                                               <  1:505795/249‑1 (MQ=255)                                        aGCGGACACAACGCCACCACGGAATAACAGCCGCTGCCCGCATCTTTGCTTTCGCTGAAATGCACCAAAATAAAACCGCACTTTTGCCAAAACGCGAGCAGTTCGGGGGTGTAACCGAAACTGACGGAAAGAAAATCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCaaag                                                                                                                                                                                                                <  1:256412/232‑4 (MQ=255)                                                caacgCCACCACGGAATAACAGCCGCTGCCCGCATCTTTGCTTTCGCTGAAATGCACCAAAATAAAACCGCACTTTTGCCAAAACGCGAGCAGTTCGGGGGTGTAACCGAAACTGACGGAAAGAAAATCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAAt                                                                                                                                                                                      >  1:687759/5‑250 (MQ=255)                                                caacgCCACCACGGAATAACAGCCGCTGCCCGCATCTTTGCTTTCGCTGAAATGCACCAAAATAAAACCGCACTTTTGCCAAAACGCGAGCAGTTCGGGGGTGTAACCGAAACTGACGGAAAGAAAATCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATc                                                                                                                                                                                     <  1:827358/247‑1 (MQ=255)                                                caacgCCACCACGGAATAACAGCCGCTGCCCGCATCTTTGCTTTCGCTGAAATGCACCAAAATAAAACCGCACTTTTGCCAAAACGCGAGCAGTTCGGGGGTGTAACCGAAACTGACGGAAAGAAAATCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATc                                                                                                                                                                                     <  1:85014/247‑1 (MQ=255)                                                           cGGAATAACAGCCGCTGCCCGCATCTTTGCTTTCGCTGAAATGCACCAAAATAAAACCGCACTTTTGCCAAAACGCGAGCAGTTCGGGGGTGTAACCGAAACTGACGGAAAGAAAATCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATcc                                                                                                                                                                                    <  1:808127/241‑1 (MQ=255)                                                                                cATCTTTGCTTTCGCTGAAATGCACCAAAATAAAACCGCACTTTTGCCAAAACGCGAGCAGTTCGGGGGTGTAACCGAAACTGACGGAAAGAAAATCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCaaa                                                                                                                                                     >  1:159577/1‑251 (MQ=255)                                                                                              cTGAAATGCACCAAAATAAAACCGCACTTTTGCCAAAACGCGAGCAGTTCGGGGGTGTAACCGAAACTGACGGAAAGAAAATCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAAttgccgcgcaggcg                                                                                                                                       <  1:27586/251‑13 (MQ=255)                                                                                                       cccAAAATAAAACCGCACTTTTGCCAAAACGCGAGCAGTTCGGGGGTGTAACCGAAACTGACGGAAAGAAAATCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTcc                                                                                                                              <  1:101494/250‑1 (MQ=255)                                                                                                           aaaTAAAACCGCACTTTTGCCAAAACGCGAGCAGTTCGGGGGTGTAACCGAAACTGACGGAAAGAAAATCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAac                                                                                                                                                                         >  1:127976/1‑204 (MQ=255)                                                                                                           aaaTAAAACCGCACTTTTGCCAAAACGCGAGCAGTTCGGGGGTGTAACCGAAACTGACGGAAAGAAAATCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCcgcgt                                                                                                                          >  1:135587/1‑250 (MQ=255)                                                                                                                aaaCCGCACTTTTGCCAAAACGCGAGCAGTTCGGGGGTGTAACCGAAACTGACGGAAAGAAAATCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATAc                                                                                                                                                                                                                               >  1:423762/1‑145 (MQ=255)                                                                                                                                          cagttCGGGGGTGTAACCGAAACTGACGGAAAGAAAATCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATc                                                                                                                                                                                     <  1:287907/157‑1 (MQ=255)                                                                                                                                          cagttCGGGGGTGTAACCGAAACTGACGGAAAGAAAATCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGcatcatcctcaa                                                                                           >  1:305880/5‑240 (MQ=255)                                                                                                                                            gttCGGGGGTGTAACCGAAACTGACGGAAAGAAAATCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCAc                                                                                                                                                                                                                                       >  1:704660/3‑109 (MQ=255)                                                                                                                                            gttCGGGGGTGTAACCGAAACTGACGGAAAGAAAATCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAACCACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGcatcatcctcaaat                                                                                         >  1:446276/3‑238 (MQ=255)                                                                                                                                            gttCGGGGGTGTAACCGAAACTGACGGAAAGAAAATCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACAt                                                                                                                                                                  <  1:340791/176‑1 (MQ=255)                                                                                                                                            gttCGGGGGTGTAACCGAAACTGACGGAAAGAAAATCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAAtttgac                                                                                                                >  1:143746/3‑225 (MQ=255)                                                                                                                                            gttCGGGGGTGTAACCGAAACTGACGGAAAGAAAATCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGcatcatcctcaaat                                                                                         >  1:578174/3‑238 (MQ=255)                                                                                                                                            gttCGGGGGTGTAACCGAAACTGACGGAAAGAAAATCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGcatcatcctcaaat                                                                                         >  1:543915/3‑238 (MQ=255)                                                                                                                                            gttCGGGGGTGTAACCGAAACTGACGGAAAGAAAATCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGcatcatcctcaaat                                                                                         >  1:836462/3‑238 (MQ=255)                                                                                                                                            gttCGGGGGTGTAACCGAAACTGACGGAAAGAAAATCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGcatcatcctcaaat                                                                                         >  1:149617/3‑238 (MQ=255)                                                                                                                                            gttCGGGGGTGTAACCGAAACTGACGGAAAGAAAATCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGcatcatcctcaaat                                                                                         >  1:217141/3‑238 (MQ=255)                                                                                                                                            gttCGGGGGTGTAACCGAAACTGACGGAAAGAAAATCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGcat                                                                                                    <  1:366912/238‑3 (MQ=255)                                                                                                                                             ttCGGGGGTGTAACCGAAACTGACGGAAAGAAAATCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAACCGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGcatcatcctcaaatc                                                                                        <  1:770770/250‑15 (MQ=255)                                                                                                                                                 ggggTGTAACCGAAACTGACGGAAAGAAAATCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGcat                                                                                                    >  1:848980/1‑233 (MQ=255)                                                                                                                                                     tgtAACCGAAACTGACGGAAAGAAAATCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGcatcatcctcaaatcctccttc                                                                                 <  1:338311/250‑22 (MQ=255)                                                                                                                                                                   aCGGAAAGAAAGTCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCGGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATGCGGGAAATGCGCAAAGATCGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCcaca                                                                   <  1:85052/250‑1 (MQ=255)                                                                                                                                                                   aCGGAAAGAAAATCCACCGCAGGTTGTTGTTTAA‑TCGGTTCGATTAAACGTGGCCCCAGCCCTCGCTGCGGCCAGTTGGGCTGCACGGCGATACGGAAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCcacac                                                                  <  1:16077/251‑1 (MQ=255)                                                                                                                                                                   aCGGAAAGAAAATCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCcacac                                                                  <  1:598662/251‑1 (MQ=255)                                                                                                                                                                    cGGAAAGAAAATCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGcatca                                                                                                  >  1:390200/1‑214 (MQ=255)                                                                                                                                                                      gaaagaaaaTCCCCCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCcacacac                                                                <  1:30271/250‑1 (MQ=255)                                                                                                                                                                      gaaagaaaaTCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCcacacac                                                                <  1:892622/250‑1 (MQ=255)                                                                                                                                                                             aaTCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCcaac                                                        <  1:386119/251‑1 (MQ=255)                                                                                                                                                                               cccACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCcaacag                                                      <  1:308059/250‑2 (MQ=255)                                                                                                                                                                               cccACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCcaacag                                                      <  1:596187/250‑2 (MQ=255)                                                                                                                                                                                     gCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAAttgc                                                                                                                                                 <  1:569874/154‑3 (MQ=255)                                                                                                                                                                                       aCGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCcacacac                                                                <  1:297852/233‑1 (MQ=255)                                                                                                                                                                                       aCGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCcaacagggtttcc                                               >  1:91252/1‑242 (MQ=255)                                                                                                                                                                                        cGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCcacac                                                                  <  1:323252/230‑1 (MQ=255)                                                                                                                                                                                          ttgttgttTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTcc                                                                                                                              <  1:489061/168‑1 (MQ=255)                                                                                                                                                                                          ttgttgttTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCcacacac                                                                >  1:471552/1‑230 (MQ=255)                                                                                                                                                                                          ttgttgttTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCcacac                                                                  >  1:437368/1‑228 (MQ=255)                                                                                                                                                                                          ttgttgttTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCcaacagggtttcccgcg                                           >  1:585054/1‑239 (MQ=255)                                                                                                                                                                                            gttgttTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCcaacagggtttcccgcgtt                                         >  1:144890/1‑237 (MQ=255)                                                                                                                                                                                               tgtttaatctgTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGcat                                                                                                    <  1:325522/180‑3 (MQ=255)                                                                                                                                                                                                gtttaatctgTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGcatcatcc                                                                                               <  1:487161/185‑8 (MQ=255)                                                                                                                                                                                                gtttaatctgTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCcacacac                                                                >  1:539036/10‑225 (MQ=255)                                                                                                                                                                                                            cGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCaaaataaaat                          >  1:445071/1‑251 (MQ=255)                                                                                                                                                                                                              aTTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTTCACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCcaacagggtttcccgcgtttgtgcaaaat                               >  1:9417/1‑220 (MQ=255)                                                                                                                                                                                                              aTTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCcaacagggtttcccgcg                                           <  1:790218/232‑13 (MQ=255)                                                                                                                                                                                                              aTTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAAttg                        >  1:143521/1‑251 (MQ=255)                                                                                                                                                                                                                         ccccAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTCgcggtg             >  1:759887/1‑249 (MQ=255)                                                                                                                                                                                                                              gccctcGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGcatca                                                                                                  >  1:227295/6‑157 (MQ=255)                                                                                                                                                                                                                                cctcGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTCGCGGTGCATCCaa      <  1:781539/248‑1 (MQ=255)                                                                                                                                                                                                                                 ctcGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTCGCGGTGCATCCaat     <  1:232337/249‑1 (MQ=255)                                                                                                                                                                                                                                 ctcGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTCGCGGTGCATCCaat     <  1:483325/249‑1 (MQ=255)                                                                                                                                                                                                                                    gctgctGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTCGCGGTGCATCCaataat  >  1:69320/1‑251 (MQ=255)                                                                                                                                                                                                                                     ctgctgCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCAAAGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTGCCGCGCAGGCGACGTTGTCCGCGTCGAATTTGACGAATTAATGCATCATCCTCAAATCCTCCTTCAGGCACCGCCCACACACAGCCCAACAGGGTTTCCCGCGTTTGTGCAAAATAAAATTGTTGTCGCGGTGCATCCaa      <  1:789812/246‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                           TGCTCCGCCTGTTGCACCAACGCCCGTCCGCGCTCCGTCAGCGGGCACAATGCCACTACGGAATAACAGCCGCTGCCTGCATCTTTGCCTTCGCTGAAATGCACCAAAATAAAACCGCACTTTTGCCAAAACGCCAGCAGCTCGGGGGTGTAACCGAAACTGACGGAAAGGAAATCCACCGCACGTTGTTGTTTAACTC‑GTTCGATTAAACGTTGCCCCAGTCCCCGCTGCTGCCAGTTGGGTTGCACGGCGATACGGGAAATGCGCAGTGAACGCAATTTACACGCCTGTTCCAATCCCGCCTGAAAACACAACATTTGCGCCACCAAATTACCACGTGGACGACGTTGTCCGCGCCGAATTTTACGAATTAATGCGTCGTCACCAAATTTGCCCTCAGGCACCGCCCACACACAACCCAACAAGGCCTCCTGCGTCTGCGCAAGATAAAATTGTTGTCGCGGCGCATCCAATAAT  >  1/1474462‑1474938

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑