Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	203,822	G→A	intergenic (+12/‑255)	unknown → / → unknown	Multidrug resistance transporter => Bicyclomycin resistance protein Bcr‑1/ABC transporter involved in cytochrome c biogenesis, ATPase component CcmA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	203,822	0	G	A	100.0%	177.3 / NA	65	intergenic (+12/‑255)	unknown/unknown	Multidrug resistance transporter => Bicyclomycin resistance protein Bcr‑1/ABC transporter involved in cytochrome c biogenesis, ATPase component CcmA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (22/43);  total (22/43)

TGTTGCTGAATTTGGGCTTTTGGGCAATTGCAATTGGCATTGCGATTTTTGTGGGACAAAACTCGCTGATTTCTTCCAACGCCATGGCGTCCATTTTAGAAAAATTCCCGAATGCCGCCTGCTCGGCAAATTCTATGGTCGGTAGTGTACGTTTCGGCACCGGCGCCTGCGTGGGA‑CACTGGTTGCCCTTATGAATATGAGCAGCGCAACGCCAATGTTGCTGACCATGGCGGCGTGTTCGTTGATGGCGGTGGTTTGCTATTATTTTTTAACCGCGCGCAATTTATAATTTTAAAAAGCGCATTATTGTAATCTGCAACGGACAGATAAA‑TTTGTTCTAAATCAAAAATATCTCAAAGTACCGTGAAAGAGTTATATGATTTTGTACTTTCCCGCCTACGCCGTTCCTTCACCTTATGATAGAATGCCGCGCTAAAATCGGTT  >  1/203617‑204060                                                                                                                                                                                                               |                                                                                                                                                                                                                                                caaattgctcgctccgctacagggCAATTGCAATTGGCATTGCGATTTTTGTGGGACAAAACTCGCTGATTTCTTCCAACGTCATGGCGTCCATTTTAGAAAAATTCCCGAATGCCGCCGGCTCGGCAAATTCTATGGTCGGTAGTGTACGTTTCGGCACCGGCGCCTGCGTGGGATCACTGGTTGCCCTTATGAATATGAGCAGCACAACGCCAATGTTGCTGACCATGGCGGCGTGTTCGTTGATGGCg                                                                                                                                                                                                     <  1:446493/230‑1 (MQ=255)            ctccgctacagggCAATTGCAATTGTCATTGCGATTTTTGTGGGACAAAACTCGCTGATTTCTTCCAACGCCATGGCGTCCATTTTAGAAAAATTCCCGAATGCCGCCGGCTCGGCAAATTCTATGGTCGGTAGTGTACTTTTCGGCACCGGCGCCTGCGTGGGATCACTGGTTGCCCTTATGAATATGAGCAGCACAACGCCACTGTTGCTGACCATGGCTGCGTGTTCGTTGATGGCGGTGGTTTGCta                                                                                                                                                                                          >  1:464602/11‑251 (MQ=255)            ctccgctacagggCAATTGCAATTGGCATTGCGATTTTTGTGGGACAAAACTCGCTGATTTCTTCCAACGCCATGGCGTCCATTTTAGAAAAATTCCCGAATGCCGCCGGCTCGGCAAATTCTATGGTCGGTAGTGTACGTTTCGGCACCGGCGCCTGCGTGGGATCACTGGTTGCCCTTATGAATATGAGCAGCACAACGCCAATGTTGCTGACCATGGCGGCGTGTTCGTTGATGGCGGTGGTTTGCta                                                                                                                                                                                          >  1:764158/11‑251 (MQ=255)            ctccgctacagggCAATTGCAATTGGCATTGCGATTTTTGTGGGACAAAACTCGCTGATTTCTTCCAACGCCATGGCGTCCATTTTAGAAAAATTCCCGAATGCCGCCGGCTCGGCAAATTCTATGGTCGGTAGTGTACGTTTCGGCACCGGCGCCTGCGTGGGATCACTGGTTGCCCTTATGAATATGAGCAGCACAACGCCAATGTTGCTGACCATGGCGGCGTGTTCGTTGATGGCGGTGGTTTGCta                                                                                                                                                                                          <  1:205039/241‑1 (MQ=255)                 ctacagggCAATTGCAATTGGCATTGCGATTTTTGTGGGACAAAACTCGCTGATTTCTTCCAACGCCATGGCGTCCATTTTAGAAAAATTCCCGAATGCCGCCGGCTCGGCAAATTCTATGGTCGGTAGTGTACGTTTCGGCACCGGCGCCTGCGTGGGATCACTGGTTGCCCTTATGAATATGAGCAGCACAACGCCAATGTTGCTGACCATggc                                                                                                                                                                                                                        <  1:315677/211‑1 (MQ=255)                 ctacagggCAATTGCAATTGGCATTGCGATTTTTGTGGGACAAAACTCGCTGATTTCTTCCAACGCCATGGCGTCCATTTTAGAAAAATTCCCGAATGCCGCCGGCTCGGCAAATTCTATGGTCGGTAGTGTACGTTTCGGCACCGGCGCCTGCGTGGGATCACTGGTTGCCCTTATGAATATGAGCAGCACAACGCCAATGTTGCTGACCATGGCGGCGTGTTCGtt                                                                                                                                                                                                            <  1:861/223‑1 (MQ=255)                 ctacagggCAATTGCAATTGGCATTGCGATTTTTGTGGGACAAAACTCGCTGATTTCTTCCAACGCCATGGCGTCCATTTTAGAAAAATTCCCGAATGCCGCCGGCTCGGCAAATTCTATGGTCGGTAGTGTACGTTTCGGCACCGGCGCCTGCGTGGGATCACTGGTTGCCCTTATGAATATGAGCAGCACAACGCCAATGTTGCTGACCATGGCGGCGTGTTCGTTGATGGCGGTGGTTTGCTattatt                                                                                                                                                                                     >  1:30640/6‑251 (MQ=255)                         caattgcaattgGCATTGCGATTTTTGTGGGACAAAACTCGCTGATTTCTTCCAACGCCATGGCGTCCATTTTAGAAAAATTCCCGAATGCCGCCGGCTCGGCAAATTCTATGGTCGGTAGTGTACGTTTCGGCACCGGCGCCTGCGTGGGATCACTGGTTGCCCTTATGAATATGAGCAGCACAACGCCAATGTTGCTGACCATGGCGGCGTGTTCGTTGATGGCGGTGGTTTGCTATTATTTTTTAAc                                                                                                                                                                              <  1:692873/250‑1 (MQ=255)                              gcaattgGCATTGCGATTTTTGTGGGACAAAACTCGCTGATTTCTTCCAACGCCATGGCGTCCATTTTAGAAAAATTCCCGAATGCCGCCGGCTCGGCAAATTCTATGGTCGGTAGTGTACGTTTCGGCACCGGCGCCTGCGTGGGATCACTGGTTGCCCTTATGAATATGAGCAGCACAACGCCAATGt                                                                                                                                                                                                                                     <  1:509933/190‑1 (MQ=255)                                         tGCGATTTTTGTGGGACAAAACTCGCTGATTTCTTCCAACGCCATGGCGTCCATTTTAGAAAAATTCCCGAATGCCGCCGGCTCGGCAAATTCTATGGTCGGTAGTGTACGTTTCGGCACCGGCGCCTGCGTGGGATCACTGGTTGCCCTTATGAATATGAGCAGCACAACGCCAATGTTGCTGACCATGGCGGCGTGTTCGTTGATGGCGGTGGTTTGCTATTATTTTTTAACCGCGCGCAATTTATAAt                                                                                                                                                             <  1:507999/251‑1 (MQ=255)                                         tGCGATTTTTGTGGGACAAAACTCGCTGATTTCTTCCAACGCCATGGCGTCCATTTTAGAAAAATTCCCGAATGCCGCCGGCTCGGCAAATTCTATGGTCGGTAGTGTACGTTTCGGCACCGGCGCCTGCGTGGGATCACTGGTTGCCCTTATGAATATGAGCAGCACAACGCCAATGTTGCTGACCATGGCGGCGTGTTCGTTGATGGCGGTGGTTTGCTATTATTTTTTAACCGCGCGCAATTTATAAt                                                                                                                                                             <  1:328278/251‑1 (MQ=255)                                                          aaaaCTCGCTGATTTCTTCCAACGCCATGGCGTCCATTTTAGAAAAATTCCCGAATGCCGCCGGCTCGGCAAATTCTATGGTCGGTAGTGTACGTTTCGGCACCGGCGCCTGCGTGGGATCACTGGTTGCCCTTATGAATATGAGCAGCACAACGCCAATGTTGCTGACCATGGCGGCGTGTTCGTTGATGGCGGTGGTTTGCTATTATTTTTTAACCGCGCGCAATTTATAATTTTAAAAAGCGCattat                                                                                                                                            <  1:58209/251‑1 (MQ=255)                                                                     aTTTCTTCCAACGCCATGGCGTCCATTTTAGAAAAATTCCCGAATGCCGCCGGCTCGGCAAATTCTATGGTCGGTAGTGTACGTTTCGGCACCGGCGCCTGCGTGGGATCACTGGTTGCCCTTATGAATATGAGCAGCACAACGCCAATGTTGCTGACCATggc                                                                                                                                                                                                                        >  1:59172/1‑164 (MQ=255)                                                                      tttCTTCCAACGCCATGGCGTCCATTTTAGAAAAATTCCCGAATGCCGCCGGCTCGGCAAATTCTATGGTCGGTAGTGTACGTTTCGGCACCGGCGCCTGCGTGGGATCACTGGTTGCCCTTATGAATATGAGCAGCACAACGCCAATGTTGCTGACCATGGCGGCGTGTTCGTTGATGGCGGTGGTTTGCTATTATTTTTTAACCGCGCGCAATTTATAATTTTAAAAAGCGCATTATTGTAATCTGCaa                                                                                                                                <  1:740735/251‑1 (MQ=255)                                                                         cttcCAACGCCATGGCGTCCATTTTAGAAAAATTCCCGAATGCCGCCGGCTCGGCAAATTCTATGGTCGGTAGTGTACGTTTCGGCACCGGCGCCTGCGTGGGATCACTGGTTGCCCTTATGAATATGAGCAGCACAACGCCAATGTTGCTGACCATGGCGGCGTGTTCGTTGATGGCGGTGGTTTGCTATTATTTTTTAACCGCGCGCAATTTATAAt                                                                                                                                                             >  1:458119/1‑219 (MQ=255)                                                                         cttcCAACGCCATGGCGTCCATTTTAGAAAAATTCCCGAATGCCGCCGGCTCGGCAAATTCTATGGTCGGTAGTGTACGTTTCGGCACCGGCGCCTGCGTGGGATCACTGGTTGCCCTTATGAATATGAGCAGCACAACGCCAATGTTGCTGACCATGGCGGCGTGTTCGTTGATGGCGGTGGTTTGCTATTATTTTTTAACCGCGCGCAATTTATAATTTTAAAAAGCGCATTATTGTaa                                                                                                                                       >  1:46054/1‑241 (MQ=255)                                                                         cttcCAACGCCATGGCGTCCATTTTAGAAAAATTCCCGAATGCCGCCGGCTCGGCAAATTCTATGGTCGGTAGTGTACGTTTCGGCACCGGCGCCTGCGTGGGATCACTGGTTGCCCTTATGAATATGAGCAGCACAACGCCAATGTTGCTGACCATGGCGGCGTGTTCGTTGATGGCGGTGGTTTGCTATTATTTTTTAACCGCGCGCAATTTATAATTTTAAAAAGCGCATTATTGTAATCTTCAACgg                                                                                                                             >  1:582164/1‑251 (MQ=255)                                                                           tcCAACGCCATGGCGTCCATTTTAGAAAAATTCCCGAATGCCGCCGGCTCGGCAAATTCTATGGTCGGTAGTGTACGTTTCGGCACCGGCGCCTGCGTGGGATCACTGGTTGCCCTTATGAATATGAGCAGCACAACGCCAATGTTGCTGACCATGGCGGCGTGTTCGTTGATGGCGGTGGTTTGCTATTATTTTTTAACCGCGCGCAATTTATAATTTTAAAAAGCGCATTATTGTAATCTGCAACGGAc                                                                                                                           <  1:708083/251‑1 (MQ=255)                                                                                    aTGGCGTCCATTTTAGAAAAATTCCCGAATGCCGCCGGCTCGGCAAATTCTATGGTCGGTAGTGTACGTTTCGGCACCGGCGCCTGCGTGGGATCACTGGTTGCCCTTATGAATATGAGCAGCACAACGCCAATGTTGCTGACCATGGCGGCGTGTTCGTTGATGGCGGTGGTTTGCTATTATTTTTTAACCGCGCGCAATTTATAATTTTAAAAAGCGCATTATTGTAATc                                                                                                                                     >  1:612092/1‑232 (MQ=255)                                                                                    aTGGCGTCCACTTTAGAAGAATTCTCGAATGCTGCCGGCTCGGCAAGTTCTATGGTCGGTAGTGTACGTTTCGGCACCGGCGCCTGCGTGGGATCACTGGTTGCCCTTATGAATATGAGCAGCACATCTGCAATGTTGCTGACCATGGCGGCGTGTTCGTTGATGGCGGTGGTTTGCTATTATTTTTTAACCGCGCGCAATTTATAATTTTAAAAAGCGCATTATTGTAATCTGCAACGGACAGATAAA‑tt                                                                                                                 <  1:585574/251‑1 (MQ=255)                                                                                     tGGCGTCCATTTTAGAAAAATTCCCGAATGCCGCCGGCTCGGCAAATTCTATGGTCGGTAGTGTACGTTTCGGCACCGGCGCCTGCGTGGGATCACTGGTTGCCCTTATGAATATGAGCAGCACAACGCCAATGTTGCTGACCATGGCGGCGTGTTCGTTGATGGCGGTGGTTTGCTATTATTTTTTAACCGCGCGCAATTTATAATTTTAAAAAGCGCATTATTGTAATCTGCAACGGACAGATAAA‑ttt                                                                                                                <  1:705535/251‑1 (MQ=255)                                                                                                    aaaaaTTCCCGAATGCCGCCGGCTCGGCAAATTCTATGGTCGGTAGTGTACGTTTCGGCACCGGCGCCTGCGTGGGATCACTGGTTGCCCTTATGAATATGAGCAGCACAACGCCAATATTGCTGACCATGGCGGCGTGTTCGTTGATGGCGGTGGTTTGCTATTATTTTTTAACCGCGCGCAATTTATAATTTTAAAAAGCGCATTATTGTAATCTGCAACGGACAGATAAA‑TTTGTTCTAAATCaaaaa                                                                                                 <  1:55149/251‑1 (MQ=255)                                                                                                           cccGAATGCCGCCGGCTCGGCAAATTCTATGGTCGGTAGTGTACGTTTCGGCACCGGCGCCTGCGTGGGATCACTGGTTGCCCTTATGAATATGAGCAGCACAACGCCAATGTTGCTGACCATGGCGGCGTGTTCGTTGATGGCGGTGGTTTGCTATTATTTTTTAACCGCGCGCAATTTATAATTTTaaaa                                                                                                                                                      <  1:590240/192‑1 (MQ=255)                                                                                                               aaTGCCGCCGGCTCGGCAAATTCTATGGTCGGTAGTGTACGTTTCGGCACCGGCGCCTGCGTGGGATCACTGGTTGCCCTTATGAATATGAGCAGCACAACGCCAATGTTGCTGACCATGGCGGCGTGTTCGTTGATGGCGGTGGTTTGCTATTATTTTTTAACCGCGCGCAATTTATAATTTTAAAAAGCGCATTATTGTAATCTGCAACGGACAGATAAA‑TTTGTTCTAAATCAAAAATATCTCAAAg                                                                                       <  1:842539/250‑1 (MQ=255)                                                                                                                 tGCCGCCGGCTCGGCAAATTCTATGGTCGGTAGTGTACGTTTCGGCACCGGCGCCTGCGTGGGATCACTGGTTGCCCTTATGAATATGAGCAGCACAACGCCAATGTTGCTGACCATGGCGGCGTGTTCGTTGATGGCGGTGGTTTGCTATTATTTTTTAACCGCGCGCAATTTATAATTTTAAAAAGCGCATTATTGTAATCTGCAACGGACAGATAAA‑TTTGTTCTAAATCAAAAATATCTCAAAGTAc                                                                                    <  1:655250/251‑1 (MQ=255)                                                                                                                            cGGCAAATTCTATGGTCGGTAGTGTACGTTTCGGCACTGGCGCCTGCGTGGGATCACTGGTTGCCCTTATGAATATGAGCAGCACAACGCCAATGTTGCTGACCATGGCGGCGTGTTCGTTGATGGCGGAGGTTTGCTATTATTTTTTAAc                                                                                                                                                                              >  1:673694/1‑151 (MQ=255)                                                                                                                                aaaTTCTATGGTCGGTAGTGTACGTTTCGGCACCGGCGCCTGCGTGGGATCACTGGTTGCCCTTATGAATATGAGCAGCACAATGCCAATGTTGCTGACCATGGCGGCGTGTTCGTTGATGGCGGTGGTTTGCTATTATTTTTTAACCGCGCGCAATTTATAATTTTAAAAAGCGCATTATTGTAATCTGCAACGGACAGATAAA‑TTTGTTCTAAATCAAAAATATCTCAAAGTACCGTGAAAGAGTtata                                                                     <  1:43259/251‑1 (MQ=255)                                                                                                                                  aTTCTATGGTCGGTAGTGTACGTTTCGGCACCGGCGCCTGCGTGGGATCACTGGTTGCCCTTATGAATATGAGCAGCACAACGCCAATGTTGCTGACCATGGCGGCGTGTTCGTTGATGGCGGTGGTTTGCTATTATTTTTTAACCGCGCGCAATTTATAATTTTAAAAAGCGCATTATTGTAATCTGCAACGGACAGATa                                                                                                                      >  1:8348/1‑201 (MQ=255)                                                                                                                                  aTTCTATGGTCGGTAGTGTACGTTTCGGCACCGGCGCCTGCGTGGGATCACTGGTTGCCCTTATGAATATGAGCAGCACAACGCCAATGTTGCTGACCATGGCGGCGTGTTCGTTGATGGCGGTGGTTTGCTATTATTTTTTAACCGCGCGCAATTTATAATTTTAAAAAGCGCATTATTGTAATCTGCAACGGACAGATAAA‑ttt                                                                                                                >  1:474187/1‑206 (MQ=255)                                                                                                                                   ttCTATGGTCGGTAGTGTACGTTTCGGCACCGGCGCCTGCGTGGGATCACTGGTTGCCCTTATGAATATGAGCAGCACAACGCCAATGTTGCTGACCATGGCGGCGTGTTCGTTGATGGCGGTGGTTTGCTATTATTTTTTAACCGCGCGCAATTTATAATTTTAAAAAGCGCATTATTGTAATCTGCAACGGACAGATAAA‑TTTGTTCTAAATCAAAAATATCTCAAAGt                                                                                      >  1:76078/1‑231 (MQ=255)                                                                                                                                         ggTCGGTAGTGTACGTTTCGGCACCGGCGCCTGCGTGGGATCACTGGTTGCCCTTATGAATATGAGCAGCACAACGCCAATGTTGCTGACCATGGCGGCGTGTTCGTTGATGGCGGTGGTTTGCta                                                                                                                                                                                          <  1:244868/126‑1 (MQ=255)                                                                                                                                         ggTCGGTAGTGTACGTTTCGGCACCGGCGCCTGCGTGGGATCACTGGTTGCCCTTATGAATATGAGCAGCACAACGCCAATGTTGCTGACCATGGCGGCGTGTTCGTTGATGGCGGTGGTTTGCTATTATTTTTTAACCGCGCGCAATTTATAATTTTAAAAAGCGCATTATTGTAATc                                                                                                                                     <  1:190305/179‑1 (MQ=255)                                                                                                                                           tCGGTAGTGTACGTTTCGGCACCGGCGCCTGCGTGGGATCACTGGTTGCCCTTATGAATATGAGCAGCACAACGCCAATGTTGCTGACCATGGCGGCGTGTTCGTTGATGGCGGTGGTTTGCTATTATTTTTTAACCGCGCGCAATTTATAATTTTAAAAAGCGCATTATTGTAATCTGCAACGGACAGATAAA‑TTTGTTCTAAATCAAAAATATCTCAAAGTACCGTGAAAGAGTTATATGATTTTGTAc                                                          <  1:50599/251‑1 (MQ=255)                                                                                                                                              gTAGTGTACGTTTCGGCACCGGCGCCTGCGTGGGATCACTGGTTGCCCTTATGAATATGAGCAGCACAACGCCAATGTTGCTGACCATGGCGGCGTGTTCGTTGATGGCGGTGGTTTGCTATTATTTTTTAAc                                                                                                                                                                              <  1:547594/133‑1 (MQ=255)                                                                                                                                              gTAGTGTACGTTTCGGCACCGGCGCCTGCGTGGGATCACTGGTTGCCCTTATGAATATGAGCAGCACAACGCCAATGTTGCTGACCATGGCGGCGTGTTCGTTGATGGCGGTGGTTTGCTATTATTTTTTAACCGCGCGCAATTTATAATTTTAAAAAg                                                                                                                                                    <  1:534670/159‑1 (MQ=255)                                                                                                                                              gTAGTGTACGTTTCGGCACCGGCGCCTGCGTGGGATCACTGGTTGCCCTTATGAATATGAGCAGCACAACGCCAATGTTGCTGACCATGGCGGCGTGTTCGTTGATGGCGGTGGTTTGCTATTATTTTTTAACCGCGCGCAATTTATAATTTTAAAAAGCGCATTATTGTAATCTGCAACGGACAGATa                                                                                                                      <  1:378585/189‑1 (MQ=255)                                                                                                                                                 gtgtACGTTTCGGCACCGGCGCCTGCGTGGGATCACTGGTTGCCCTTATGAATATGAGCAGCACAACGCCAATGTTGCTGACCATGGCGGCGTGTTCGTTGATGGCGGTGGTTTGCTATTATTTTTTAACCGCGCGCAATTTATAAt                                                                                                                                                             <  1:294532/147‑1 (MQ=255)                                                                                                                                                 gtgtACGTTTCGGCACCGGCGCCTGCGTGGGATCACTGGTTGCCCTTATGAATATGAGCAGCACAACGCCAATGTTGCTGACCATGGCGGCGTGTTCGTTGATGGCGGTGGTTTGCTATTATTTTTTAACCGCGCGCAATTTATAATTTTAAAAAGCGCATTATTGTAATCTGCAACGGACAGATAAA‑TTTGTTCTAAATCAAAAATATCTCAAAGt                                                                                      <  1:415463/217‑1 (MQ=255)                                                                                                                                                  tgtACGTTTCGGCACCGGCGCCTGCGTGGGATCACTGGTTGCCCTTATGAATATGAGCAGCACAACGCCAATGTTGCTGACCATGGCGGCGTGTTCGTTGATGGCGGTGGTTTGCTATTATTTTTTAACCGCGCGCAATTTATAATTTTAAAAAGCGCATTATTGTAATCTGCAACGGACAGATAAA‑TTTGTTCTAAATCAAAAATATCTCAAAGTACCGTGAAAGAGTTATATGATTTTGTACTTTCCCg                                                   <  1:201927/251‑1 (MQ=255)                                                                                                                                                   gtACGTTTCGGCACCGGCGCCTGCGTGGGATCACTGGTTGCCCTTATGAATATGAGCAGCACAACGCCAATGTTGCTGACCATGGCGGCGTGTTCGTTGATGGCGGTGGTTTGCTATTATTTTTTAACCGCGCGCAATTTATAATTTTAAAAAGCGCATTATTGTAATCTGCAACGGACAGATAAA‑TTTGTTCTAAATCAAAAATATCTCAAAGTACCGTGAAAGAGTTATATg                                                                   <  1:336228/234‑1 (MQ=255)                                                                                                                                                        tttCGGCACCGGCGCCTGCGTGGGATCACTGGTTGCCCTTATGAATATGAGCAGCACAACGCCAATGTTGCTGACCATGGCGGCGTGTTCGTTGATGGCGGTGGTTTGCTATTATTTTTTAAc                                                                                                                                                                              <  1:368503/123‑1 (MQ=255)                                                                                                                                                               cccGGCGCCTGCGTGGGATCACTGGTTGCCCTTATGAATATGAGCAGCACAACGCCAATGTTGCTGACCATGGCGGCGTGTTCGTTGATGGCGGTGGTTTGCTATTATTTTTTAACCGCGCGCAATTTATAATTTTAAAAAGCGCATTATTGTAATCTGCAACGGACAGATAAA‑TTTGTTCTAAATCAAAAATATCTCAAAGTACCGTGAAAGAGTTATATGATTTTGTACTTTCCCGCCTACGCCGTTcc                                      <  1:848675/250‑1 (MQ=255)                                                                                                                                                               aCCGGCGCCTGCGTGGGATCACTGGTTGCCCTTATGAATATGAGCAGCACAACGCCAATGTTGCTGACCATGGCGGCGTGTTCGTTGATGGCGGTGGTTTGCTATTATTTTTTAACCGCGCGCAATTTATAATTTTAAAAAGCGCATTATTGTAATCTGCAACGGACAGATAAA‑TTTGTTCTAAATCAAAAATATCTCAAAGTACCGTGAAAGAGTTATATGATTTTGTACTTTCCCGCCTACGCCGTTcc                                      <  1:662919/251‑1 (MQ=255)                                                                                                                                                                        tGCGTGGGATCACTGGTTGCCCTTATGAATATGAGCAGCACAACGCCAATGTTGCTGACCATGGCGGCGTGTTCGTTGATGGCGGTGGTTTGCTATTATTTTTTAACCGCGCGCAATTTATAATTTTAAAAAGCGCATTATTGTAATCTGCAACGGACAGATAAA‑TTTGTTCt                                                                                                           <  1:183838/173‑1 (MQ=255)                                                                                                                                                                         gCGTGGGATCACTGGTTGCCCTTATGAATATGAGCAGCACAACGCCAATGTTGCTGACCATGGCGGCGTGTTCGTTGATGGCGGTGGTTTGCTATTATTTTTTAACCGCGCGCAATTTATAATTTTAAAAAGCGCATTATTGTAATCTGCAACgg                                                                                                                             <  1:110603/155‑1 (MQ=255)                                                                                                                                                                         gCGTGGGATCACTGGTTGCCCTTATGAATATGAGCAGCACAACGCCAATGTTGCTGACCATGGCGGCGTGTTCGTTGATGGCGGTGGTTTGCTATTATTTTTTAACCGCGCGCAATTTATAATTTTAAAAAGCGCATTATTGTAATCTGCAACGGACAGATAAA‑TTTGTTCTAAATCAAAAATATCTCAAAGTACCGTGAAAGAGTTATATGATTTTGTACTTTCCCGcc                                                 <  1:721439/230‑1 (MQ=255)                                                                                                                                                                         gCGTGGGATCACTGGTTGCCCTTATGAATATGAGCAGCACAACGCCAATGTTGCTGACCATGGCGGCGTGTTCGTTGATGGCGGTGGTTTGCTATTATTTTTTAACCGCGCGCAATTTATAATTTTAAAAAGCGCATTATTGTAATCTGCAACGGACAGATAAA‑TTTGTTCTAAATCAAAAATATCTCAAAGTACCGTGAAAGAGTTATATGATTTTGTACTTTCCCGCCTACGCCGTTCCTTCACCTTAt                            >  1:360717/1‑251 (MQ=255)                                                                                                                                                                            tgggatcacTGGTTGCCCTTATGAATATGAGCAGCACAACGCCAATGTTGCTGACCATGGCGGCGTGTTCGTTGATGGCGGTGGTTTGCTATTATTTTTTAACCGCGCGCAATTTATAATTTTAAAAAGCGCATTATTGTAATCTGCAACGGACAGATAAA‑TTTGTTCTAAATCAAAAATATCTCAAAGTACCGTGAAAGAGTTATATGAttt                                                               >  1:276384/7‑213 (MQ=255)                                                                                                                                                                            tgggatcacTGGTTGCCCTTATGAATATGAGCAGCACAACGCCAATGTTGCTGACCATGGCGGCGTGTTCGTTGATGGCGGTGGTTTGCTATTATTTTTTAACCGCGCGCAATTTATAATTTTAAAAAGCGCATTATTGTAATCTGCAACGGACAGATAAA‑TTTGTTCTAAATCAAAAATATCTCAAAGTACCGTGAAAGAGTTATATGATTTTGTACTTTCCCGCCTACGCCGTTCCTTCACCTTATGAt                         <  1:480970/245‑1 (MQ=255)                                                                                                                                                                            tgggatcacTGGTTGCCCTTATGAATATGAGCAGCACAACGCCAATGTTGCTGACCATGGCGGCGTGTTCGTTGATGGCGGTGGTTTGCTATTATTTTTTAACCGCGCGCAATTTATAATTTTAAAAAGCGCATTATTGTAATCTGCAACGGACAGATAAA‑TTTGTTCTAAATCAAAAATATCTCAAAGTACCGTGAAAGAGTTATATGATTTTGTACTTTCCCGCCTACGCCGTTCCTTCACCTTATGAt                         <  1:725647/245‑1 (MQ=255)                                                                                                                                                                            tgggatcacTGGTTGCCCTTATGAATATGAGCAGCACAACGCCAATGTTGCTGACCATGGCGGCGTGTTCGTTGATGGCGGTGGTTTGCTATTATTTTTTAACCGCGCGCAATTTATAATTTTAAAAAGCGCATTATTGTAATCTGCAACGGACAGATAAA‑TTTGTTCTAAATCAAAAATATCTCAAAGTACCGTGAAAGAGTTATATGATTTTGTACTTTCCCGCCTACGCCGTTCCTTCACCTTATGAt                         <  1:125432/245‑1 (MQ=255)                                                                                                                                                                              ggatcacTGGTTGCCCTTATGAATATGAGCAGCACAACGCCAATGTTGCTGACCATGGCGGCGTGTTCGTTGATGGCGGTGGTTTGCTATTATTTTTTAACCGCGCGCAATTTATAATTTTAAAAAGCGCATTATTGTAATCTGCAACGGACAGATAAA‑TTTGTTCTAAATCAAAAATATCTCAAAGTACCGTGAAAGAGTTATATGATTTTGTACTTTCCCGCCTACGCCGTTCCTTCACCTTATGATAg                       >  1:472401/5‑251 (MQ=255)                                                                                                                                                                               gatcacTGGTTGCCCTTATGAATATGAGCAGCACAACGCCAATGTTGCTGACCATGGCGGCGTGTTCGTTGATGGCGGTGGTTTGCTATTATTTTTTAACCGCGCGCAATTTATAATTTTAAAAAGCGCATTATTGTAATCTGCAACGGACAGATAAA‑TTTGTTCTAAATCAAAAATATCTCAAAGTACCGTGAAAGAGTTATATGATTTTGTACTTTCCCGCCTACGCCGTTCCTTCACCTTATGATAGa                      >  1:436167/4‑251 (MQ=255)                                                                                                                                                                                    cTGGTTGCCCTTATGAATATGAGCAGCACAACGCCAATGTTGCTGACCATGGCGGCGTGTTCGTTGATGGCGGTGGTTTGCTATTATTTTTTAACCGCGCGCAATTTATAATTTTAAAAAGCGCATTATTGTAATCTGCAACGGACAGATAAATTTTGTTCTAAATCAAAAATATCTCAAAGTACCGTGAAAGAGTTATATGATTTTGTACTTTCCCGCCTACGCCGTTCCTTCACCTTATGATAGAATGc                  <  1:189767/251‑1 (MQ=255)                                                                                                                                                                                    cTGGTTGCCCTTATGAATATGAGCAGCACAACGCCAATGTTGCTGACCATGGCGGCGTGTTCGTTGATGGCGGTGGTTTGCTATTATTTTTTAACCGCGCGCAATTTATAATTTTAAAAAGCGCATTATTGTAATCTGCAACGGACAGATAAA‑TTTGTTCTAAATCAAAAATATCTCAAAGTACCGTGAAAGAGTTATATGATTTTGTACTTTCCCGCCTACGCCGTTCCTTCACCTTATGATAGAATGc                  >  1:660220/1‑250 (MQ=255)                                                                                                                                                                                    cTGGTTGCCCTTATGAATATGAGCAGCACAACGCCAATGTTGCTGACCATGGCGGCGTGTTCGTTGATGGCGGTGGTTTGCTATTATTTTTTAACCGCGCGCAATTTATAATTTTAAAAAGCGCATTATTGTAATCTGCAACGGACAGATAAA‑TTTGTTCTAAATCAAAAATATCTCAAAGTACCGTGAAAGAGTTATATGATTTTGTACTTTCCCGCCTACGCCGTTCCTTCACCTTATGATAGAATGc                  >  1:697625/1‑250 (MQ=255)                                                                                                                                                                                           cccTTATGAATATGAGCAGCACAACGCCAATGTTGCTGACCATGGCGGCGTGTTCGTTGATGGCGGTGGTTTGCTATTATTTTTTAACCGCGCGCAATTTATAATTTTAAAAAGCGCATTATTGTAATCTGCAACGGACAGATAAA‑TTTGTTCTAAATCAAAAATATCTCAAAGTACCGTGAAAGAGTTATATGATTTTGTACTTTCCCGCCTACGCCGTTCCTTCACCTTATGAt                         <  1:102774/236‑1 (MQ=255)                                                                                                                                                                                              ttATGAATATGAGCAGCACAACGCCAATGTTGCTGAcc                                                                                                                                                                                                                             <  1:396161/38‑1 (MQ=255)                                                                                                                                                                                               tATGAATATGAGCAGCACAACGCCAATGTTGCTGACCATGGCGGCGTGTTCGTTGATGGCGGTGGTTTGCTATTATTTTTTAACCGCGCGCAATTTATAATTTTAAAAAGCGCATTATTGTAATCTGCAACGGACAGATAAA‑TTTGTTCTAAATCAAAAATATCTCAAAGTACCGTGAAAGAGTTATATGATTTTGTACTTTCCCGCCTACGc                                            <  1:645459/213‑1 (MQ=255)                                                                                                                                                                                                aTGAATATGAGCAGCACAACGCCAATGTTGCTGACCATGGCGGCGTGTTCGTTGATGGCGGTGGTTTGCTATTATTTTTTAACCGCGCGCAATTTATAATTTTAAAAAGCGCATTATTGTAATCTGCAACGGACAGATAAA‑TTTGTTCTAAATCAAAAATATCTCAAAGTACCGTGAAAGAGTTATATGATTTTGTACTTTCCCGCCTACGCCGTTCCTTCACCTTATGATAGAATGCCGCGCTAAAATCg     >  1:23661/1‑251 (MQ=255)                                                                                                                                                                                                   aaTATGAGCAGCACAACGCCAATGTTGCTGACCATGGCGGCGTGTTCGTTGATGGCGGTGGTTTGCTATTATTTTTTAACCGCGCGCAATTTATAATTTTAAAAAGCGCATTATTGTAATCTGCAACGGACAGATAAA‑TTTGTTCTAAATCAAAAATATCTCAAAGt                                                                                      <  1:91683/167‑1 (MQ=255)                                                                                                                                                                                                   aaTATGAGCAGCACAACGCCAATGTTGCTGACCATGGCGGCGTGTTCGTTGATGGCGGTGGTTTGCTATTATTTTTTAACCGCGCGCAATTTATAATTTTAAAAAGCGCATTATTGTAATCTGCAACGGACAGATAAA‑TTTGTTCTAAATCAAAAATATCTCAAAGTACCGTGAAAGAGTTATATGATTTTGTACTTTCCCGCCTACGCCGTTCCTTCACCTTATGATAGAATGCCGCGCTAAAATCGGtt  >  1:158146/1‑251 (MQ=255)                                                                                                                                                                                                    atatGAGCAGCACAACGCCAATGTTGCTGACCATGGCGGCGTGTTCGTTGATGGCGGTGGTTTGCTATTATTTTTTAACCGCGCGCAATTTATAATTTTAAAAAGCGCATTATTGTAATCTGCAACGGACAGATAAA‑TTTGTTCTAAATCAAAAATATCTCAAAGTACCGTGAAAGAGTTATATGATTTTGTACTTTCCCGCCTACGCCGTTCCTTCACCTTATGATAGAATGc                  <  1:781773/234‑1 (MQ=255)                                                                                                                                                                                                        gAGCAGCACAACGCCAATGTTGCTGACCATGGCGGCGTGTTCGTTGATGGCGGTGGTTTGCTATTATTTTTTAACCGCGCGCAATTTATAATTTTAAAAAGCGCATTATTGTAATCTGCAACGGACAGATAAA‑TTTGTTCTAAATCAAAAATATCTCAAAGTACCGTGAAagag                                                                          >  1:508389/1‑174 (MQ=255)                                                                                                                                                                                                        gAGCAGCACAACGCCAATGTTGCTGACCATGGCGGCGTGTTCGTTGATGGCGGTGGTTTGCTATTATTTTTTAACCGCGCGCAATTTATAATTTTAAAAAGCGCATTATTGTAATCTGCAACGGACAGATAAA‑TTTGTTCTAAATCAAAAATATCTCAAAGTACCGTGAAAGAGTTATATGATTTTGTACTTTCCCGCCTACGCCGTTCCTTCACCTTATGAt                         >  1:548827/1‑223 (MQ=255)                                                                                                                                                                                                               |                                                                                                                                                                                                                                                TGTTGCTGAATTTGGGCTTTTGGGCAATTGCAATTGGCATTGCGATTTTTGTGGGACAAAACTCGCTGATTTCTTCCAACGCCATGGCGTCCATTTTAGAAAAATTCCCGAATGCCGCCTGCTCGGCAAATTCTATGGTCGGTAGTGTACGTTTCGGCACCGGCGCCTGCGTGGGA‑CACTGGTTGCCCTTATGAATATGAGCAGCGCAACGCCAATGTTGCTGACCATGGCGGCGTGTTCGTTGATGGCGGTGGTTTGCTATTATTTTTTAACCGCGCGCAATTTATAATTTTAAAAAGCGCATTATTGTAATCTGCAACGGACAGATAAA‑TTTGTTCTAAATCAAAAATATCTCAAAGTACCGTGAAAGAGTTATATGATTTTGTACTTTCCCGCCTACGCCGTTCCTTCACCTTATGATAGAATGCCGCGCTAAAATCGGTT  >  1/203617‑204060

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑