Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,474,500	C→T	L526L (CTG→CTA)	unknown ←	tRNA cytosine(34) acetyltransferase (EC 2.3.1.193)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,474,500	0	C	T	100.0%	162.0 / NA	57	L526L (CTG→CTA)	unknown	tRNA cytosine(34) acetyltransferase (EC 2.3.1.193)
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (23/34);  total (23/34)

GCTGTCAATAAAGGAAAATCCCGCTCCCCGCTTAATTTCATCAAGCGCCGAATAGCGGGCAAGGCAAACGCCAACGGCAGGAAGAAATCGGCAAAATCCGCCAAGCATTGCCAATCGGTTTCATTGAGCGACCAATCCGTTTCGGTTCCGACAAATTGCGTCGCCAGCGGGTGCAACGAAAGGGGCAGATTACGTTTAAATTGTTGCTCCGCCTGTTGCACCAACGCCCGTCCGCGCTCCGTCAGCGGGCACAATGCCACTACGGAATAACAGCCGCTGCCTGCATCTTTGCCTTCGCTGAAATGCACCAAAATAAAACCGCACTTTTGCCAAAACGCCAGCAGCTCGGGGGTGTAACCGAAACTGACGGAAAGGAAATCCACCGCACGTTGTTGTTTAACTC‑GTTCGATTAAACGTTGCCCCAGTCCCCGCTGCTGCCAGTTGGGTTGCACGGCGATACGGGAAATGCGCAGTGA  >  1/1474258‑1474733                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                           gCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCggaca                                                                                                                                                                                                                                    >  1:410682/1‑248 (MQ=255)          aaaGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCAc                                                                                                                                                                                                                           <  1:332777/251‑1 (MQ=255)            aGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCAcca                                                                                                                                                                                                                         >  1:616559/1‑249 (MQ=255)              gAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCAccac                                                                                                                                                                                                                        <  1:214156/250‑4 (MQ=255)              gAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCAccac                                                                                                                                                                                                                        <  1:457608/250‑4 (MQ=255)                 aatcctgttCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAGCGCCACCACGGaa                                                                                                                                                                                                                    <  1:728512/243‑1 (MQ=255)                       gttCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCACCACGGAATAACAg                                                                                                                                                                                                              >  1:205980/3‑251 (MQ=255)                        ttCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCACCACGGAATAACAg                                                                                                                                                                                                              <  1:23021/249‑1 (MQ=255)                        ttCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCACCACGGAATAACAg                                                                                                                                                                                                              <  1:548670/249‑1 (MQ=255)                        ttCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCACCACGGAATAACAg                                                                                                                                                                                                              <  1:858142/249‑1 (MQ=255)                           cccGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCACCACGGAATAACAGCCGc                                                                                                                                                                                                          <  1:323976/251‑1 (MQ=255)                              gCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCAc                                                                                                                                                                                                                           >  1:752862/1‑231 (MQ=255)                                 tactttCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCACCACGGAATAACAGCCGctgcccg                                                                                                                                                                                                    >  1:458958/4‑249 (MQ=255)                                   ctttCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCACCACGGAATAACAGCCGCTGCCCGCa                                                                                                                                                                                                  >  1:88031/2‑251 (MQ=255)                                                                     cGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCACCACGGAATAACAGCCGCTGCCCGCATCTTTGCTTTCGCTGAAATGCACCAAAATAAAAc                                                                                                                                                                <  1:139986/251‑1 (MQ=255)                                                                     cGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCACCACGGAATAACAGCCGCTGCCCGCATCTTTGCTTTCGCTGAAATGCACCAAAATAAAAc                                                                                                                                                                <  1:550499/251‑1 (MQ=255)                                                                           cggtaGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTGGCGGACACAACGCCACCACGGAATAACAGCCGCTGCCCGCATCTTTGCTTTCGCTGAAATGCACCAAAATAAAACCGCa                                                                                                                                                            >  1:429372/5‑249 (MQ=255)                                                                           cggtaGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCACCACGGAATAACAGCCGCTGCCCGCATCTTTGCTTTCGCTGAAATGCACCAAAATAAAACCGCa                                                                                                                                                            >  1:672984/5‑249 (MQ=255)                                                                           cggtaGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCACCACGGAATAACAGCCGCTGCCCGCATCTTTGCTTTCGCTGAAATGCACCAAAATAAAACCGCa                                                                                                                                                            >  1:639961/5‑249 (MQ=255)                                                                             gtaGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCACCACGGAATAACAGCCGCTGCCCGCATCTTTGCTTTCGCTGAAATGCACCAAAATAAAACCGCACttt                                                                                                                                                        >  1:35151/3‑251 (MQ=255)                                                                                 gaagaaATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCACCACGGAATAACAGCCGCTGCCCGCATCTTTGCTTTCGCTGAAATGCACCAAAATAAAACCGCACTTTTGc                                                                                                                                                     >  1:383121/1‑250 (MQ=255)                                                                                                   cgccaaaccttgccggtCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCACCACGGAATAACAGCCGCTGCCCGCATCTTTGCTTTCGCTGAAATGCACCAAAATAAAACCGCACTTTTGCCAAAACGCGAGCAGTTCgg                                                                                                                                  >  1:41675/17‑251 (MQ=255)                                                                                                     ccaaaccttgccggtCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCACCACGGAATAACAGCCGCTGCCCGCATCTTTGCTTTCGCTGAAATGCACCAAAATAAAACCGCACTTTTGCCAAAACGCGAGCAGTTCggg                                                                                                                                 <  1:748746/236‑1 (MQ=255)                                                                                                      caaaccttgccggtCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCACCACGGAATAACAGCCGCTGCCCGCATCTTTGCTTTCGCTGAAATGCACCAAAATAAAACCGCACTTTTGCCAAAACGCGAGCAGTTCggggg                                                                                                                               <  1:38965/238‑1 (MQ=255)                                                                                                      caaaccttgccggtCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCACCACGGAATAACAGCCGCTGCCCGCATCTTTGCTTTCGCTGAAATGCACCAAAATAAAACCGCACTTTTGCCAAAACGCGAGCAGTTCggggg                                                                                                                               <  1:497213/238‑1 (MQ=255)                                                                                                      caaaccttgccggtCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCACCACGGAATAACAGCCGCTGCCCGCATCTTTGCTTTCGCTGAAATGCACCAAAATAAAACCGCACTTTTGCCAAAACGCGAGCAGTTCggggg                                                                                                                               <  1:766329/238‑1 (MQ=255)                                                                                                      caaaccttgccggtCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCACCACGGAATAACAGCCGCTGCCCGCATCTTTGCTTTCGCTGAAATGCACCAAAATAAAACCGCACTTTTGCCAAAACGCGAGCAGTTCggggg                                                                                                                               <  1:271732/238‑1 (MQ=255)                                                                                                      caaacctggccggtCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCACCACGGAATAACAGCCGCTGCCCGCATCTTTGCTTTCGCTGAAATGCACCAAAATAAAACCGCACTTTTGCCAAAACGCGAGCAGTTCggggg                                                                                                                               <  1:855603/238‑1 (MQ=255)                                                                                                                   tCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCACCACGGAATAACAGCCGCTGCCCGCATCTTTGCTTTCGCTGAAATGCACCAAAATAAAACCGCACTTTTGCCAAAACGCGAGCAGTTCGGGGGTGTAACCGAAAc                                                                                                                   <  1:622352/250‑1 (MQ=255)                                                                                                                      gcttCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCACCACGGAATAACAGCCGCTGCCCGCATCTTTGCTTTCGCTGAAATGCACCAAAATAAAACCGCACTTTTGCCAAAACGCGAGCAGTTCggggg                                                                                                                               >  1:571006/3‑235 (MQ=255)                                                                                                                       cttCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCACCACGGAATAACAGCCGCTGCCCGCATCTTTGCTTTCGCTGAAATGCACCAAAATAAAACCGCACTTTTGCCAAAACGCGAGCAGTTCGGGGGTGTAACCGAAACTGACg                                                                                                              >  1:8584/2‑251 (MQ=255)                                                                                                                         tCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCACCACGGAATAACAGCCGCTGCCCGCATCTTTGCTTTCGCTGAAATGCACCAAAATAAAACCGCACTTTTGCCAAAACGCGAGCAGTTCGGGGGTGTAACCGAAACTGACgga                                                                                                            >  1:25636/1‑251 (MQ=255)                                                                                                                                   aCCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCACCACGGAATAACAGCCGCTGCCCGCATCTTTGCTTTCGCTGAAATGCACCAAAATAAAACCGCACTTTTGCCAAAACGCGAGCAGTTCGGGGGTGTAACCGAAACTGACGGAAAGAAAATc                                                                                                   >  1:28006/1‑250 (MQ=255)                                                                                                                                                  ttCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCACCACGGAATAACAGCCGCTGCCCGCATCTTTGCTTTCGCTGAAATGCACCAAAATAAAACCGCACTTTTGCCAAAACGCGAGCAGTTCGGGGGTGTAACCGAAACTGACGGAAAGAAAATCCACCGCACgttgttgt                                                                                   <  1:394151/251‑1 (MQ=255)                                                                                                                                                    ccggcAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCACCACGGAATAACAGCCGCTGCCCGCATCTTTGCTTTCGCTGAAATGCACCAAAATAAAACCGCACTTTTGCCAAAACGCGAGCAGTTCggggg                                                                                                                               >  1:780018/5‑205 (MQ=255)                                                                                                                                                      ggcAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCACCACGGAATAACAGCCGCTGCCCGCATCTTTGCTTTCGCTGAAATGCACCAAAATAAAACCGCACTTTTGCCAAAACGCGAGCAGTTCGGGGGTGTAACCGAAACTGACGGAAAGAAAATCCACCGCACGTTGTTGTTTaa                                                                               >  1:422809/3‑251 (MQ=255)                                                                                                                                                        cAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCACCACGGAATAACAGCCGCTGCCCGCATCTTTGCTTTCGCTGAAATGCACCAAAATAAAACCGCACTTTTGCCAAAACGCGAGCAGTTCGGGGGTGTAACCGAAACTGACGGAAAGAAAATCCACCGCACGTTGTTGTTTaatc                                                                             <  1:140263/251‑3 (MQ=255)                                                                                                                                                        cAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCACCACGGAATAACAGCCGCTGCCCGCATCTTTGCTTTCGCTGAAATGCACCAAAATAAAACCGCACTTTTGCCAAAACGCGAGCAGTTCGGGGGTGTAACCGAAACTGACGGAAAGAAAATCCACCGCACGTTGTTGTTTaatc                                                                             <  1:559844/251‑3 (MQ=255)                                                                                                                                                        cAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCACCACGGAATAACAGCCGCTGCCCGCATCTTTGCTTTCGCTGAAATGCACCAAAATAAAACCGCACTTTTGCCAAAACGCGAGCAGTTCGGGGGTGTAACCGAAACTGACGGAAAGAAAATCCACCGCACGTTGTTGTTTaatc                                                                             <  1:807808/251‑3 (MQ=255)                                                                                                                                                                        cGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCACCACGGAATAACAGCCGCTGCCCGCATCTTTGCTTTCGCTGAAATGCACCAAAATAAAACCGCACTTTTGCCAAAACGCGAGCAGTTCGGGGGTGTAACCGAAACTGACGGAAAGAAAATCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGt                                                             <  1:810302/250‑1 (MQ=255)                                                                                                                                                                          ggtacaatgAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCACCACGGAATAACAGCCGCTGCCCGCATCTTTGCTTTCGCTGAAATGCACCAAAATAAAACCGCACTTTTGCCAAAACGCGAGCAGTTCGGGGGTGTAACCGAAACTGACGGAAAGAAAATCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTg                                                           >  1:709743/9‑250 (MQ=255)                                                                                                                                                                          ggTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCACCACGGAATAACAGCCGCTGCCCGCATCTTTGCTTTCGCTGAAATGCACCAAAATAAAACCGCACTTTTGCCAAAACGCGAGCAGTTCGGGGGTGTAACCGAAAc                                                                                                                   <  1:497447/195‑1 (MQ=255)                                                                                                                                                                            tGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCACCACGGAATAACAGCCGCTGCCCGCATCTTTGCTTTCGCTGAAATGCACCAAAATAAAACCGCACTTTTGCCAAAACGCGAGCAGTTCGGGGGTGTAACCGAAACTGACGGAAAGAAAATCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGccc                                                        <  1:476885/251‑1 (MQ=255)                                                                                                                                                                             gCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCACCACGGAATAACAGCCGCTGCCCGCATCTTTGCTTTCGCTGAAATGCACCAAAATAAAACCGCACTTTTGCCAAAACGCGAGCAGTTCGGGGGTGTAACCGAAACTGACGGAAAGAAAATCCACCGCACGTTGTTGTTTaa                                                                               <  1:77605/228‑1 (MQ=255)                                                                                                                                                                             gCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCACCACGGAATAACAGCCGCTGCCCGCATCTTTGCTTTCGCTGAAATGCACCAAAATAAAACCGCACTTTTGCCAAAACGCGAGCAGTTCGGGGGTGTAACCGAAACTGACGGAAAGAAAATCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGccc                                                        >  1:704049/1‑250 (MQ=255)                                                                                                                                                                              caatgAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCACCACGGAATAACAGCCGCTGCCCGCATCTTTGCTTTCGCTGAAATGCACCAAAATAAAACCGCACTTTTGCCAAAACGCGAGCAGTTCGGGGGTGTAACCGAAACTGACGGAAAGAAAATCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGt                                                             >  1:881949/5‑244 (MQ=255)                                                                                                                                                                                  gAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCACCACGGAATAACAGCCGCTGCCCGCATCTTTGCTTTCGCTGAAATGCACCAAAATAAAACCGCACTTTTGCCAAAACGCGAGCAGTTCGGGGGTGTAACCGAAACTGACGGAAAGAAAATCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAgcc                                                   >  1:447361/1‑248 (MQ=255)                                                                                                                                                                                      ggggCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCACCACGGAATAACAGCCGCTGCCCGCATCTTTGCTTTCGCTGAAATGCACCAAAATAAAACCGCACTTTTGCCAAAACGCGAGCAGTTCGGGGGTGTAACCGAAACTGACGGAAAGAAAATCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAgccctcg                                               >  1:465959/1‑244 (MQ=255)                                                                                                                                                                                         gcaaattgcGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCACCACGGAATAACAGCCGCTGCCCGCATCTTTGCTTTCGCTGAAATGCACCAAAATAAAACCGCACTTTTGCCAAAACGCGAGCAGTTCGGGGGTGTAACCGAAACTGACGGAAAGAAAATCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCgctg                                            <  1:819576/242‑1 (MQ=255)                                                                                                                                                                                             attgcGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCACCACGGAATAACAGCCGCTGCCCGCATCTTTGCTTTCGCTGAAATGCACCAAAATAAAACCGCACTTTTGCCAAAACGCGAGCAGTTCGGGGGTGTAACCGAAACTGACGGAAAGAAAATCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGcc                                       <  1:168332/247‑1 (MQ=255)                                                                                                                                                                                                     tAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCACCACGGAATAACAGCCGCTGCCCGCATCTTTGCTTTCGCTGAAATGCACCAAAATAAAACCGCACTTTTGCCAAAACGCGAGCAGTTCGGGGGTGTAACCGAAACTGACGGAAAGAAAATCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTgggc                               <  1:635730/251‑2 (MQ=255)                                                                                                                                                                                                             tgttCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCACCACGGAATAACAGCCGCTGCCCGCATCTTTGCTTTCGCTGAAATGCACCAAAATAAAACCGCACTTTTGCCAAAACGCGAGCAGTTCGGGGGTGTAACCGAAACTGACGGAAAGAAAATCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCTAGTTGGGCTGCACGGc                       <  1:562036/248‑1 (MQ=255)                                                                                                                                                                                                             tgttCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCACCACGGAATAACAGCCGCTGCCCGCATCTTTGCTTTCGCTGAAATGCACCAAAATAAAACCGCACTTTTGCCAAAACGCGAGCAGTTCGGGGGTGTAACCGAAACTGACGGAAAGAAAATCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGc                       <  1:602337/248‑1 (MQ=255)                                                                                                                                                                                                                        ttGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCACCACGGAATAACAGCCGCTGCCCGCATCTTTGCTTTCGCTGAAATGCACCAAAATAAAACCGCACTTTTGCCAAAACGCGAGCAGTTCGGGGGTGTAACCGAAACTGACGGAAAGAAAATCCAc                                                                                                <  1:315291/168‑1 (MQ=255)                                                                                                                                                                                                                                 cgcccgccCGTGCTCCGTTAGCGGACACAACGCCACCACGGAATAACAGCCGCTGCCCGCATCTTTGCTTTCGCTGAAATGCACCAAAATAAAACCGCACTTTTGCCAAAACGCGAGCAGTTCGGGGGTGTAACCGAAACTGACGGAAAGAAAATCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCaaag   <  1:289964/251‑4 (MQ=255)                                                                                                                                                                                                                                   cccgcccgTGCTCCGTTAGCGGACACAACGCCACCACGGAATAACAGCCGCTGCCCGCATCTTTGCTTTCGCTGAAATGCACCAAAATAAAACCGCACTTTTGCCAAAACGCGAGCAGTTCGGGGGTGTAACCGAAACTGACGGAAAGAAAATCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAGCCCTCGCTGCTGCCAGTTGGGCTGCACGGCGATACGGGAAATGCGCaaaga  <  1:369461/250‑5 (MQ=255)                                                                                                                                                                                                                                       cccgtgcTCCGTTAGCGGACACAACGCCACCACGGAATAACAGCCGCTGCCCGCATCTTTGCTTTCGCTGAAATGCACCAAAATAAAACCGCACTTTTGCCAAAACGCGAGCAGTTCGGGGGTGTAACCGAAACTGACGGAAAGAAAATCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGt                                                             <  1:528975/182‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                           GCTGTCAATAAAGGAAAATCCCGCTCCCCGCTTAATTTCATCAAGCGCCGAATAGCGGGCAAGGCAAACGCCAACGGCAGGAAGAAATCGGCAAAATCCGCCAAGCATTGCCAATCGGTTTCATTGAGCGACCAATCCGTTTCGGTTCCGACAAATTGCGTCGCCAGCGGGTGCAACGAAAGGGGCAGATTACGTTTAAATTGTTGCTCCGCCTGTTGCACCAACGCCCGTCCGCGCTCCGTCAGCGGGCACAATGCCACTACGGAATAACAGCCGCTGCCTGCATCTTTGCCTTCGCTGAAATGCACCAAAATAAAACCGCACTTTTGCCAAAACGCCAGCAGCTCGGGGGTGTAACCGAAACTGACGGAAAGGAAATCCACCGCACGTTGTTGTTTAACTC‑GTTCGATTAAACGTTGCCCCAGTCCCCGCTGCTGCCAGTTGGGTTGCACGGCGATACGGGAAATGCGCAGTGA  >  1/1474258‑1474733

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑