Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,474,449	A→G	R543R (CGT→CGC)	unknown ←	tRNA cytosine(34) acetyltransferase (EC 2.3.1.193)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,474,449	0	A	G	100.0%	194.1 / NA	68	R543R (CGT→CGC)	unknown	tRNA cytosine(34) acetyltransferase (EC 2.3.1.193)
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (35/33);  total (35/33)

TTTTTCAGCCACGTTTTTTTATGTTGAATGTCATCAGGGCGTTTGCAATAGGCTGTCAATAAAGGAAAATCCCGCTCCCCGCTTAATTTCATCAAGCGCCGAATAGCGGGCAAGGCAAACGCCAACGGCAGGAAGAAATCGGCAAAATCCGCCAAGCATTGCCAATCGGTTTCATTGAGCGACCAATCCGTTTCGGTTCCGACAAATTGCGTCGCCAGCGGGTGCAACGAAAGGGGCAGATTACGTTTAAATTGTTGCTCCGCCTGTTGCACCAACGCCCGTCCGCGCTCCGTCAGCGGGCACAATGCCACTACGGAATAACAGCCGCTGCCTGCATCTTTGCCTTCGCTGAAATGCACCAAAATAAAACCGCACTTTTGCCAAAACGCCAGCAGCTCGGGGGTGTAACCGAAACTGACGGAAAGGAAATCCACCGCACGTTGTTGTTTAACTC‑GTTCGATTAAACGTTGCCCCAGTCCCCG  >  1/1474207‑1474688                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                 tttgccaaccACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTaaa                                                                                                                                                                                                                                          <  1:739232/243‑1 (MQ=255)                tttttATTTTGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGcccg                                                                                                                                                                                                                           >  1:298862/1‑249 (MQ=255)                     attttGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCATTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCa                                                                                                                                                                                                                      >  1:442306/4‑251 (MQ=255)                       tttGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCAcc                                                                                                                                                                                                                    <  1:896113/250‑1 (MQ=255)                       tttGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCAcc                                                                                                                                                                                                                    <  1:216956/250‑1 (MQ=255)                         tGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCaa                                                                                                                                                                                                                  <  1:220851/251‑1 (MQ=255)                             tGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGc                                                                                                                                                                                                               >  1:806194/1‑250 (MQ=255)                               tcatcaGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCaa                                                                                                                                                                                                                  >  1:537666/1‑245 (MQ=255)                                catcaGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCg                                                                                                                                                                                                            >  1:750548/1‑250 (MQ=255)                                catcaGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCAGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCg                                                                                                                                                                                                            >  1:441831/1‑250 (MQ=255)                                 atcaGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCgcc                                                                                                                                                                                                          <  1:673041/251‑3 (MQ=255)                                     ggacgcttGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGcccgcccgtg                                                                                                                                                                                                      >  1:890464/7‑249 (MQ=255)                                       acgcttGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGcccgcccgtgc                                                                                                                                                                                                     <  1:46631/246‑4 (MQ=255)                                       acgcttGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGcccgcccgtgc                                                                                                                                                                                                     <  1:253026/246‑4 (MQ=255)                                        cgcttGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGccc                                                                                                                                                                                                                            <  1:463500/223‑2 (MQ=255)                                           ttGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGt                                                                                                                                                                                                >  1:475604/1‑251 (MQ=255)                                            tGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGtt                                                                                                                                                                                               >  1:551660/1‑250 (MQ=255)                                             gCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGtta                                                                                                                                                                                              >  1:664681/1‑249 (MQ=255)                                                    gCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCggaca                                                                                                                                                                                       >  1:410682/1‑248 (MQ=255)                                                             aaaGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCAc                                                                                                                                                                              <  1:332777/251‑1 (MQ=255)                                                               aGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCAcca                                                                                                                                                                            >  1:616559/1‑249 (MQ=255)                                                                 gAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCAccac                                                                                                                                                                           <  1:214156/250‑4 (MQ=255)                                                                 gAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCAccac                                                                                                                                                                           <  1:457608/250‑4 (MQ=255)                                                                    aatcctgttCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAGCGCCACCACGGaa                                                                                                                                                                       <  1:728512/243‑1 (MQ=255)                                                                          gttCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCACCACGGAATAACAg                                                                                                                                                                 >  1:205980/3‑251 (MQ=255)                                                                           ttCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCACCACGGAATAACAg                                                                                                                                                                 <  1:23021/249‑1 (MQ=255)                                                                           ttCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCACCACGGAATAACAg                                                                                                                                                                 <  1:858142/249‑1 (MQ=255)                                                                           ttCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCACCACGGAATAACAg                                                                                                                                                                 <  1:548670/249‑1 (MQ=255)                                                                              cccGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCACCACGGAATAACAGCCGc                                                                                                                                                             <  1:323976/251‑1 (MQ=255)                                                                                 gCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCAc                                                                                                                                                                              >  1:752862/1‑231 (MQ=255)                                                                                    tactttCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCAc                                                                                                                                                                                                                     >  1:788098/4‑189 (MQ=255)                                                                                    tactttCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCACCACGGAATAACAGCCGctgcccg                                                                                                                                                       >  1:458958/4‑249 (MQ=255)                                                                                      ctttCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCACCACGGAATAACAGCCGCTGCCCGCa                                                                                                                                                     >  1:88031/2‑251 (MQ=255)                                                                                                                        cGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCACCACGGAATAACAGCCGCTGCCCGCATCTTTGCTTTCGCTGAAATGCACCAAAATAAAAc                                                                                                                   <  1:139986/251‑1 (MQ=255)                                                                                                                        cGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCACCACGGAATAACAGCCGCTGCCCGCATCTTTGCTTTCGCTGAAATGCACCAAAATAAAAc                                                                                                                   <  1:550499/251‑1 (MQ=255)                                                                                                                              cggtaGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTGGCGGACACAACGCCACCACGGAATAACAGCCGCTGCCCGCATCTTTGCTTTCGCTGAAATGCACCAAAATAAAACCGCa                                                                                                               >  1:429372/5‑249 (MQ=255)                                                                                                                              cggtaGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCACCACGGAATAACAGCCGCTGCCCGCATCTTTGCTTTCGCTGAAATGCACCAAAATAAAACCGCa                                                                                                               >  1:639961/5‑249 (MQ=255)                                                                                                                              cggtaGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCACCACGGAATAACAGCCGCTGCCCGCATCTTTGCTTTCGCTGAAATGCACCAAAATAAAACCGCa                                                                                                               >  1:672984/5‑249 (MQ=255)                                                                                                                               ggtaGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAAttgttgttc                                                                                                                                                                                                                                 >  1:740332/4‑131 (MQ=255)                                                                                                                                gtaGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCACCACGGAATAACAGCCGCTGCCCGCATCTTTGCTTTCGCTGAAATGCACCAAAATAAAACCGCACttt                                                                                                           >  1:35151/3‑251 (MQ=255)                                                                                                                                    gaagaaATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCACCACGGAATAACAGCCGCTGCCCGCATCTTTGCTTTCGCTGAAATGCACCAAAATAAAACCGCACTTTTGc                                                                                                        >  1:383121/1‑250 (MQ=255)                                                                                                                                                      cgccaaaccttgccggtCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCACCACGGAATAACAGCCGCTGCCCGCATCTTTGCTTTCGCTGAAATGCACCAAAATAAAACCGCACTTTTGCCAAAACGCGAGCAGTTCgg                                                                                     >  1:41675/17‑251 (MQ=255)                                                                                                                                                        ccaaaccttgccggtCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCACCACGGAATAACAGCCGCTGCCCGCATCTTTGCTTTCGCTGAAATGCACCAAAATAAAACCGCACTTTTGCCAAAACGCGAGCAGTTCggg                                                                                    <  1:748746/236‑1 (MQ=255)                                                                                                                                                         caaaccttgccggtCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCACCACGGAATAACAGCCGCTGCCCGCATCTTTGCTTTCGCTGAAATGCACCAAAATAAAACCGCACTTTTGCCAAAACGCGAGCAGTTCggggg                                                                                  <  1:271732/238‑1 (MQ=255)                                                                                                                                                         caaaccttgccggtCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCACCACGGAATAACAGCCGCTGCCCGCATCTTTGCTTTCGCTGAAATGCACCAAAATAAAACCGCACTTTTGCCAAAACGCGAGCAGTTCggggg                                                                                  <  1:766329/238‑1 (MQ=255)                                                                                                                                                         caaaccttgccggtCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCACCACGGAATAACAGCCGCTGCCCGCATCTTTGCTTTCGCTGAAATGCACCAAAATAAAACCGCACTTTTGCCAAAACGCGAGCAGTTCggggg                                                                                  <  1:497213/238‑1 (MQ=255)                                                                                                                                                         caaaccttgccggtCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCACCACGGAATAACAGCCGCTGCCCGCATCTTTGCTTTCGCTGAAATGCACCAAAATAAAACCGCACTTTTGCCAAAACGCGAGCAGTTCggggg                                                                                  <  1:38965/238‑1 (MQ=255)                                                                                                                                                         caaacctggccggtCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCACCACGGAATAACAGCCGCTGCCCGCATCTTTGCTTTCGCTGAAATGCACCAAAATAAAACCGCACTTTTGCCAAAACGCGAGCAGTTCggggg                                                                                  <  1:855603/238‑1 (MQ=255)                                                                                                                                                                      tCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCACCACGGAATAACAGCCGCTGCCCGCATCTTTGCTTTCGCTGAAATGCACCAAAATAAAACCGCACTTTTGCCAAAACGCGAGCAGTTCGGGGGTGTAACCGAAAc                                                                      <  1:622352/250‑1 (MQ=255)                                                                                                                                                                         gcttCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCACCACGGAATAACAGCCGCTGCCCGCATCTTTGCTTTCGCTGAAATGCACCAAAATAAAACCGCACTTTTGCCAAAACGCGAGCAGTTCggggg                                                                                  >  1:571006/3‑235 (MQ=255)                                                                                                                                                                          cttCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCACCACGGAATAACAGCCGCTGCCCGCATCTTTGCTTTCGCTGAAATGCACCAAAATAAAACCGCACTTTTGCCAAAACGCGAGCAGTTCGGGGGTGTAACCGAAACTGACg                                                                 >  1:8584/2‑251 (MQ=255)                                                                                                                                                                            tCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCACCACGGAATAACAGCCGCTGCCCGCATCTTTGCTTTCGCTGAAATGCACCAAAATAAAACCGCACTTTTGCCAAAACGCGAGCAGTTCGGGGGTGTAACCGAAACTGACgga                                                               >  1:25636/1‑251 (MQ=255)                                                                                                                                                                                      aCCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCACCACGGAATAACAGCCGCTGCCCGCATCTTTGCTTTCGCTGAAATGCACCAAAATAAAACCGCACTTTTGCCAAAACGCGAGCAGTTCGGGGGTGTAACCGAAACTGACGGAAAGAAAATc                                                      >  1:28006/1‑250 (MQ=255)                                                                                                                                                                                                     ttCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCACCACGGAATAACAGCCGCTGCCCGCATCTTTGCTTTCGCTGAAATGCACCAAAATAAAACCGCACTTTTGCCAAAACGCGAGCAGTTCGGGGGTGTAACCGAAACTGACGGAAAGAAAATCCACCGCACgttgttgt                                      <  1:394151/251‑1 (MQ=255)                                                                                                                                                                                                       ccggcAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCACCACGGAATAACAGCCGCTGCCCGCATCTTTGCTTTCGCTGAAATGCACCAAAATAAAACCGCACTTTTGCCAAAACGCGAGCAGTTCggggg                                                                                  >  1:780018/5‑205 (MQ=255)                                                                                                                                                                                                         ggcAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCACCACGGAATAACAGCCGCTGCCCGCATCTTTGCTTTCGCTGAAATGCACCAAAATAAAACCGCACTTTTGCCAAAACGCGAGCAGTTCGGGGGTGTAACCGAAACTGACGGAAAGAAAATCCACCGCACGTTGTTGTTTaa                                  >  1:422809/3‑251 (MQ=255)                                                                                                                                                                                                           cAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCACCACGGAATAACAGCCGCTGCCCGCATCTTTGCTTTCGCTGAAATGCACCAAAATAAAACCGCACTTTTGCCAAAACGCGAGCAGTTCGGGGGTGTAACCGAAACTGACGGAAAGAAAATCCACCGCACGTTGTTGTTTaatc                                <  1:807808/251‑3 (MQ=255)                                                                                                                                                                                                           cAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCACCACGGAATAACAGCCGCTGCCCGCATCTTTGCTTTCGCTGAAATGCACCAAAATAAAACCGCACTTTTGCCAAAACGCGAGCAGTTCGGGGGTGTAACCGAAACTGACGGAAAGAAAATCCACCGCACGTTGTTGTTTaatc                                <  1:140263/251‑3 (MQ=255)                                                                                                                                                                                                           cAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCACCACGGAATAACAGCCGCTGCCCGCATCTTTGCTTTCGCTGAAATGCACCAAAATAAAACCGCACTTTTGCCAAAACGCGAGCAGTTCGGGGGTGTAACCGAAACTGACGGAAAGAAAATCCACCGCACGTTGTTGTTTaatc                                <  1:559844/251‑3 (MQ=255)                                                                                                                                                                                                                           cGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCACCACGGAATAACAGCCGCTGCCCGCATCTTTGCTTTCGCTGAAATGCACCAAAATAAAACCGCACTTTTGCCAAAACGCGAGCAGTTCGGGGGTGTAACCGAAACTGACGGAAAGAAAATCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGt                <  1:810302/250‑1 (MQ=255)                                                                                                                                                                                                                             ggtacaatgAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCACCACGGAATAACAGCCGCTGCCCGCATCTTTGCTTTCGCTGAAATGCACCAAAATAAAACCGCACTTTTGCCAAAACGCGAGCAGTTCGGGGGTGTAACCGAAACTGACGGAAAGAAAATCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTg              >  1:709743/9‑250 (MQ=255)                                                                                                                                                                                                                             ggTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCACCACGGAATAACAGCCGCTGCCCGCATCTTTGCTTTCGCTGAAATGCACCAAAATAAAACCGCACTTTTGCCAAAACGCGAGCAGTTCGGGGGTGTAACCGAAAc                                                                      <  1:497447/195‑1 (MQ=255)                                                                                                                                                                                                                               tGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCACCACGGAATAACAGCCGCTGCCCGCATCTTTGCTTTCGCTGAAATGCACCAAAATAAAACCGCACTTTTGCCAAAACGCGAGCAGTTCGGGGGTGTAACCGAAACTGACGGAAAGAAAATCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGccc           <  1:476885/251‑1 (MQ=255)                                                                                                                                                                                                                                gCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCACCACGGAATAACAGCCGCTGCCCGCATCTTTGCTTTCGCTGAAATGCACCAAAATAAAACCGCACTTTTGCCAAAACGCGAGCAGTTCGGGGGTGTAACCGAAACTGACGGAAAGAAAATCCACCGCACGTTGTTGTTTaa                                  <  1:77605/228‑1 (MQ=255)                                                                                                                                                                                                                                gCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCACCACGGAATAACAGCCGCTGCCCGCATCTTTGCTTTCGCTGAAATGCACCAAAATAAAACCGCACTTTTGCCAAAACGCGAGCAGTTCGGGGGTGTAACCGAAACTGACGGAAAGAAAATCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGccc           >  1:704049/1‑250 (MQ=255)                                                                                                                                                                                                                                 caatgAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCACCACGGAATAACAGCCGCTGCCCGCATCTTTGCTTTCGCTGAAATGCACCAAAATAAAACCGCACTTTTGCCAAAACGCGAGCAGTTCGGGGGTGTAACCGAAACTGACGGAAAGAAAATCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGt                >  1:881949/5‑244 (MQ=255)                                                                                                                                                                                                                                     gAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCACCACGGAATAACAGCCGCTGCCCGCATCTTTGCTTTCGCTGAAATGCACCAAAATAAAACCGCACTTTTGCCAAAACGCGAGCAGTTCGGGGGTGTAACCGAAACTGACGGAAAGAAAATCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAgcc      >  1:447361/1‑248 (MQ=255)                                                                                                                                                                                                                                         ggggCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCGGACACAACGCCACCACGGAATAACAGCCGCTGCCCGCATCTTTGCTTTCGCTGAAATGCACCAAAATAAAACCGCACTTTTGCCAAAACGCGAGCAGTTCGGGGGTGTAACCGAAACTGACGGAAAGAAAATCCACCGCACGTTGTTGTTTAA‑TCTGTTCGATTAAACGTTGCCCCAgccctcg  >  1:465959/1‑244 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                 TTTTTCAGCCACGTTTTTTTATGTTGAATGTCATCAGGGCGTTTGCAATAGGCTGTCAATAAAGGAAAATCCCGCTCCCCGCTTAATTTCATCAAGCGCCGAATAGCGGGCAAGGCAAACGCCAACGGCAGGAAGAAATCGGCAAAATCCGCCAAGCATTGCCAATCGGTTTCATTGAGCGACCAATCCGTTTCGGTTCCGACAAATTGCGTCGCCAGCGGGTGCAACGAAAGGGGCAGATTACGTTTAAATTGTTGCTCCGCCTGTTGCACCAACGCCCGTCCGCGCTCCGTCAGCGGGCACAATGCCACTACGGAATAACAGCCGCTGCCTGCATCTTTGCCTTCGCTGAAATGCACCAAAATAAAACCGCACTTTTGCCAAAACGCCAGCAGCTCGGGGGTGTAACCGAAACTGACGGAAAGGAAATCCACCGCACGTTGTTGTTTAACTC‑GTTCGATTAAACGTTGCCCCAGTCCCCG  >  1/1474207‑1474688

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑