Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	203,736	T→G	C342G (TGC→GGC)	unknown →	Multidrug resistance transporter => Bicyclomycin resistance protein Bcr‑1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	203,736	0	T	G	100.0%	80.7 / NA	31	C342G (TGC→GGC)	unknown	Multidrug resistance transporter => Bicyclomycin resistance protein Bcr‑1
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (12/19);  total (12/19)

TGTTGCTGAATTTGGGCTTTTGGGCAATTGCAATTGGCATTGCGATTTTTGTGGGACAAAACTCGCTGATTTCTTCCAACGCCATGGCGTCCATTTTAGAAAAATTCCCGAATGCCGCCTGCTCGGCAAATTCTATGGTCGGTAGTGTACGTTTCGGCACCGGCGCCTGCGTGGGA‑CACTGGTTGCCCTTATGAATATGAGCAGCGCAACGCCAATGTTGCTGACCATGGCGGCGTGTTCGTTGATGGCGGTGGTTTGCTATTATTTTTTAACCGCGCGCAATTTATAATTTTAAAAAGCGCATTATTGTAATCTGCAACGGACAGATAAATTTGTTCTAAATCAAAAATATCTCAAAGTAC  >  1/203617‑203978                                                                                                                        |                                                                                                                                                                                                                                                    caaattgctcgctccgctacagggCAATTGCAATTGGCATTGCGATTTTTGTGGGACAAAACTCGCTGATTTCTTCCAACGTCATGGCGTCCATTTTAGAAAAATTCCCGAATGCCGCCGGCTCGGCAAATTCTATGGTCGGTAGTGTACGTTTCGGCACCGGCGCCTGCGTGGGATCACTGGTTGCCCTTATGAATATGAGCAGCACAACGCCAATGTTGCTGACCATGGCGGCGTGTTCGTTGATGGCg                                                                                                                  <  1:446493/230‑1 (MQ=255)          cgctccgctacagggCAATTGCAATTGGCATTGCGATTTTTGTGGGACAAAACTCGCTGATTTCTTCCAACGCCATGGCGTCCATTTTAGAAAAATTCCCGAATGCCGCCGGCTCGGCAAATTCTATGGTCGGTAGTGTACGTTTCGGCACCGGCGCCTGCGTGGGATCACTg                                                                                                                                                                                       <  1:193484/161‑1 (MQ=255)            ctccgctacagggCAATTGCAATTGTCATTGCGATTTTTGTGGGACAAAACTCGCTGATTTCTTCCAACGCCATGGCGTCCATTTTAGAAAAATTCCCGAATGCCGCCGGCTCGGCAAATTCTATGGTCGGTAGTGTACTTTTCGGCACCGGCGCCTGCGTGGGATCACTGGTTGCCCTTATGAATATGAGCAGCACAACGCCACTGTTGCTGACCATGGCTGCGTGTTCGTTGATGGCGGTGGTTTGCta                                                                                                       >  1:464602/11‑251 (MQ=255)            ctccgctacagggCAATTGCAATTGGCATTGCGATTTTTGTGGGACAAAACTCGCTGATTTCTTCCAACGCCATGGCGTCCATTTTAGAAAAATTCCCGAATGCCGCCGGCTCGGCAAATTCTATGGTCGGTAGTGTACGt                                                                                                                                                                                                                     >  1:279897/11‑141 (MQ=255)            ctccgctacagggCAATTGCAATTGGCATTGCGATTTTTGTGGGACAAAACTCGCTGATTTCTTCCAACGCCATGGCGTCCATTTTAGAAAAATTCCCGAATGCCGCCGGCTCGGCAAATTCTATGGTCGGTAGTGTACGTTTCGGCACCGGCGCCTGCGTGGGATCACTGGTTGCCCTTATGAATATGAGCAGCACAACGCCAATGTTGCTGACCATGGCGGCGTGTTCGTTGATGGCGGTGGTTTGCta                                                                                                       <  1:205039/241‑1 (MQ=255)            ctccgctacagggCAATTGCAATTGGCATTGCGATTTTTGTGGGACAAAACTCGCTGATTTCTTCCAACGCCATGGCGTCCATTTTAGAAAAATTCCCGAATGCCGCCGGCTCGGCAAATTCTATGGTCGGTAGTGTACGTTTCGGCACCGGCGCCTGCGTGGGATCACTGGTTGCCCTTATGAATATGAGCAGCACAACGCCAATGTTGCTGACCATGGCGGCGTGTTCGTTGATGGCGGTGGTTTGCta                                                                                                       >  1:764158/11‑251 (MQ=255)              ccgctacagggCAATTGCAATTGGCATTGCGATTTTTGTGGGACAAAACTCGCTGATTTCTTCCAACGCCATGGCGTCCATTTTAGAAAAATTCCCGAATGCCGCCGGCTCGGCAAATTCTATGGTCGGt                                                                                                                                                                                                                              >  1:382534/9‑130 (MQ=255)                 ctacagggCAATTGCAATTGGCATTGCGATTTTTGTGGGACAAAACTCGCTGATTTCTTCCAACGCCATGGCGTCCATTTTAGAAAAATTCCCGAATGCCGCCGGCTCGGCAAATTCTATGGTCGGTAGTGTAc                                                                                                                                                                                                                       <  1:256123/129‑1 (MQ=255)                 ctacagggCAATTGCAATTGGCATTGCGATTTTTGTGGGACAAAACTCGCTGATTTCTTCCAACGCCATGGCGTCCATTTTAGAAAAATTCCCGAATGCCGCCGGCTCGGCAAATTCTATGGTCGGTAGTGTACGTTTCGGCACCGGCGCCTGCGTGGGATCACTGGTTGCCCTTATGAATATGAGCAGCACAACGCCAATGTTGCTGACCATggc                                                                                                                                     <  1:315677/211‑1 (MQ=255)                 ctacagggCAATTGCAATTGGCATTGCGATTTTTGTGGGACAAAACTCGCTGATTTCTTCCAACGCCATGGCGTCCATTTTAGAAAAATTCCCGAATGCCGCCGGCTCGGCAAATTCTATGGTCGGTAGTGTACGTTTCGGCACCGGCGCCTGCGTGGGATCACTGGTTGCCCTTATGAATATGAGCAGCACAACGCCAATGTTGCTGACCATGGCGGCGTGTTCGtt                                                                                                                         <  1:861/223‑1 (MQ=255)                 ctacagggCAATTGCAATTGGCATTGCGATTTTTGTGGGACAAAACTCGCTGATTTCTTCCAACGCCATGGCGTCCATTTTAGAAAAATTCCCGAATGCCGCCGGCTCGGCAAATTCTATGGTCGGTAGTGTACGTTTCGGCACCGGCGCCTGCGTGGGATCACTGGTTGCCCTTATGAATATGAGCAGCACAACGCCAATGTTGCTGACCATGGCGGCGTGTTCGTTGATGGCGGTGGTTTGCTattatt                                                                                                  >  1:30640/6‑251 (MQ=255)                         caattgcaattgGCATTGCGATTTTTGTGGGACAAAACTCGCTGATTTCTTCCAACGCCATGGCGTCCATTTTAGAAAAATTCCCGAATGCCGCCGGCTCGGCAAATTCTATGGTCGGTAGTGTACGTTTCGGCACCGGCGCCTGCGTGGGATCACTGGTTGCCCTTATGAATATGAGCAGCACAACGCCAATGTTGCTGACCATGGCGGCGTGTTCGTTGATGGCGGTGGTTTGCTATTATTTTTTAAc                                                                                           <  1:692873/250‑1 (MQ=255)                              gcaattgGCATTGCGATTTTTGTGGGACAAAACTCGCTGATTTCTTCCAACGCCATGGCGTCCATTTTAGAAAAATTCCCGAATGCCGCCGGCTCGGCAAATTCTATGGTCGGTAGTGTACGTTTCGGCACCGGCGCCTGCGTGGGATCACTGGTTGCCCTTATGAatat                                                                                                                                                                      >  1:274086/1‑170 (MQ=255)                              gcaattgGCATTGCGATTTTTGTGGGACAAAACTCGCTGATTTCTTCCAACGCCATGGCGTCCATTTTAGAAAAATTCCCGAATGCCGCCGGCTCGGCAAATTCTATGGTCGGTAGTGTACGTTTCGGCACCGGCGCCTGCGTGGGATCACTGGTTGCCCTTATGAATATGagc                                                                                                                                                                  >  1:110978/1‑174 (MQ=255)                              gcaattgGCATTGCGATTTTTGTGGGACAAAACTCGCTGATTTCTTCCAACGCCATGGCGTCCATTTTAGAAAAATTCCCGAATGCCGCCGGCTCGGCAAATTCTATGGTCGGTAGTGTACGTTTCGGCACCGGCGCCTGCGTGGGATCACTGGTTGCCCTTATGAATATGAGCAGCACAACGCCAATGt                                                                                                                                                  <  1:509933/190‑1 (MQ=255)                                         tGCGATTTTTGTGGGACAAAACTCGCTGATTTCTTCCAACGCCATGGCGTCCATTTTAGAAAAATTCCCGAATGCCGCCGGCTCGGCAAATTCTATGGTCGGTAGTGTACGTTTCGGCACCGGCGCCTGCGTGGGATCACTGGTTGCCCTTATGAATATGAGCAGCACAACGCCAATGTTGCTGACCATGGCGGCGTGTTCGTTGATGGCGGTGGTTTGCTATTATTTTTTAACCGCGCGCAATTTATAAt                                                                          <  1:328278/251‑1 (MQ=255)                                         tGCGATTTTTGTGGGACAAAACTCGCTGATTTCTTCCAACGCCATGGCGTCCATTTTAGAAAAATTCCCGAATGCCGCCGGCTCGGCAAATTCTATGGTCGGTAGTGTACGTTTCGGCACCGGCGCCTGCGTGGGATCACTGGTTGCCCTTATGAATATGAGCAGCACAACGCCAATGTTGCTGACCATGGCGGCGTGTTCGTTGATGGCGGTGGTTTGCTATTATTTTTTAACCGCGCGCAATTTATAAt                                                                          <  1:507999/251‑1 (MQ=255)                                                          aaaaCTCGCTGATTTCTTCCAACGCCATGGCGTCCATTTTAGAAAAATTCCCGAATGCCGCCGGCTCGGCAAATTCTATGGTCGGTAGTGTACGTTTCGGCACCGGCGCCTGCGTGGGATCACTGGTTGCCCTTATGAATATGAGCAGCACAACGCCAATGTTGCTGACCATGGCGGCGTGTTCGTTGATGGCGGTGGTTTGCTATTATTTTTTAACCGCGCGCAATTTATAATTTTAAAAAGCGCattat                                                         <  1:58209/251‑1 (MQ=255)                                                                     aTTTCTTCCAACGCCATGGCGTCCATTTTAGAAAAATTCCCGAATGCCGCCGGCTCGGCAAATTCTATGGTCGGTAGTGTACGTTTCGGCACCGGCGCCTGCGTGGGATCACTGGTTGCCCTTATGAATATGAGCAGCACAACGCCAATGTTGCTGACCATggc                                                                                                                                     >  1:59172/1‑164 (MQ=255)                                                                      tttCTTCCAACGCCATGGCGTCCATTTTAGAAAAATTCCCGAATGCCGCCGGCTCGGCAAATTCTATGGTCGGTAGTGTACGTTTCGGCACCGGCGCCTGCGTGGGATCACTGGTTGCCCTTATGAATATGAGCAGCACAACGCCAATGTTGCTGACCATGGCGGCGTGTTCGTTGATGGCGGTGGTTTGCTATTATTTTTTAACCGCGCGCAATTTATAATTTTAAAAAGCGCATTATTGTAATCTGCaa                                             <  1:740735/251‑1 (MQ=255)                                                                         cttcCAACGCCATGGCGTCCATTTTAGAAAAATTCCCGAATGCCGCCGGCTCGGCAAATTCTATGGTCGGTAGTGTACGTTTCGGCACCGGCGCCTGCGTGGGATCACTGGTTGCCCTTATGAATATGAGCAGCACAACGCCAATGTTGCTGACCATGGCGGCGTGTTCGTTGATGGCGGTGGTTTGCTATTATTTTTTAACCGCGCGCAATTTATAAt                                                                          >  1:458119/1‑219 (MQ=255)                                                                         cttcCAACGCCATGGCGTCCATTTTAGAAAAATTCCCGAATGCCGCCGGCTCGGCAAATTCTATGGTCGGTAGTGTACGTTTCGGCACCGGCGCCTGCGTGGGATCACTGGTTGCCCTTATGAATATGAGCAGCACAACGCCAATGTTGCTGACCATGGCGGCGTGTTCGTTGATGGCGGTGGTTTGCTATTATTTTTTAACCGCGCGCAATTTATAATTTTAAAAAGCGCATTATTGTaa                                                    >  1:46054/1‑241 (MQ=255)                                                                         cttcCAACGCCATGGCGTCCATTTTAGAAAAATTCCCGAATGCCGCCGGCTCGGCAAATTCTATGGTCGGTAGTGTACGTTTCGGCACCGGCGCCTGCGTGGGATCACTGGTTGCCCTTATGAATATGAGCAGCACAACGCCAATGTTGCTGACCATGGCGGCGTGTTCGTTGATGGCGGTGGTTTGCTATTATTTTTTAACCGCGCGCAATTTATAATTTTAAAAAGCGCATTATTGTAATCTTCAACgg                                          >  1:582164/1‑251 (MQ=255)                                                                           tcCAACGCCATGGCGTCCATTTTAGAAAAATTCCCGAATGCCGCCGGCTCGGCAAATTCTATGGTCGGTAGTGTACGTTTCGGCACCGGCGCCTGCGTGGGATCACTGGTTGCCCTTATGAATATGAGCAGCACAACGCCAATGTTGCTGACCATGGCGGCGTGTTCGTTGATGGCGGTGGTTTGCTATTATTTTTTAACCGCGCGCAATTTATAATTTTAAAAAGCGCATTATTGTAATCTGCAACGGAc                                        <  1:708083/251‑1 (MQ=255)                                                                                    aTGGCGTCCATTTTAGAAAAATTCCCGAATGCCGCCGGCTCGGCAAATTCTATGGTCGGTAGTGTACGTTTCGGCACCGGCGCCTGCGTGGGATCACTGGTTGCCCTTATGAATATGAGCAGCACAACGCCAATGTTGCTGACCATGGCGGCGTGTTCGTTGATGGCGGTGGTTTGCTATTATTTTTTAACCGCGCGCAATTTATAATTTTAAAAAGCGCATTATTGTAATc                                                  >  1:612092/1‑232 (MQ=255)                                                                                    aTGGCGTCCACTTTAGAAGAATTCTCGAATGCTGCCGGCTCGGCAAGTTCTATGGTCGGTAGTGTACGTTTCGGCACCGGCGCCTGCGTGGGATCACTGGTTGCCCTTATGAATATGAGCAGCACATCTGCAATGTTGCTGACCATGGCGGCGTGTTCGTTGATGGCGGTGGTTTGCTATTATTTTTTAACCGCGCGCAATTTATAATTTTAAAAAGCGCATTATTGTAATCTGCAACGGACAGATAAAtt                               <  1:585574/251‑1 (MQ=255)                                                                                     tGGCGTCCATTTTAGAAAAATTCCCGAATGCCGCCGGCTCGGCAAATTCTATGGTCGGTAGTGTACGTTTCGGCACCGGCGCCTGCGTGGGATCACTGGTTGCCCTTATGAATATGAGCAGCACAACGCCAATGTTGCTGACCATGGCGGCGTGTTCGTTGATGGCGGTGGTTTGCTATTATTTTTTAACCGCGCGCAATTTATAATTTTAAAAAGCGCATTATTGTAATCTGCAACGGACAGATAAAttt                              <  1:705535/251‑1 (MQ=255)                                                                                                    aaaaaTTCCCGAATGCCGCCGGCTCGGCAAATTCTATGGTCGGTAGTGTACGTTTCGGCACCGGCGCCTGCGTGGGATCACTGGTTGCCCTTATGAATATGAGCAGCACAACGCCAATATTGCTGACCATGGCGGCGTGTTCGTTGATGGCGGTGGTTTGCTATTATTTTTTAACCGCGCGCAATTTATAATTTTAAAAAGCGCATTATTGTAATCTGCAACGGACAGATAAATTTGTTCTAAATCaaaaa               <  1:55149/251‑1 (MQ=255)                                                                                                           cccGAATGCCGCCGGCTCGGCAAATTCTATGGTCGGTAGTGTACGTTTCGGCACCGGCGCCTGCGTGGGATCACTGGTTGCCCTTATGAATATGAGCAGCACAACGCCAATGTTGCTGACCATGGCGGCGTGTTCGTTGATGGCGGTGGTTTGCTATTATTTTTTAACCGCGCGCAATTTATAATTTTaaaa                                                                   <  1:590240/192‑1 (MQ=255)                                                                                                               aaTGCCGCCGGCTCGGCAAATTCTATGGTCGGTAGTGTACGTTTCGGCACCGGCGCCTGCGTGGGATCACTGGTTGCCCTTATGAATATGAGCAGCACAACGCCAATGTTGCTGACCATGGCGGCGTGTTCGTTGATGGCGGTGGTTTGCTATTATTTTTTAACCGCGCGCAATTTATAATTTTAAAAAGCGCATTATTGTAATCTGCAACGGACAGATAAATTTGTTCTAAATCAAAAATATCTCAAAg     <  1:842539/250‑1 (MQ=255)                                                                                                                 tGCCGCCGGCTCGGCAAATTCTATGGTCGGTAGTGTACGTTTCGGCACCGGCGCCTGCGTGGGATCACTGGTTGCCCTTATGAATATGAGCAGCACAACGCCAATGTTGCTGACCATGGCGGCGTGTTCGTTGATGGCGGTGGTTTGCTATTATTTTTTAACCGCGCGCAATTTATAATTTTAAAAAGCGCATTATTGTAATCTGCAACGGACAGATAAATTTGTTCTAAATCAAAAATATCTCAAAGTAc  <  1:655250/251‑1 (MQ=255)                                                                                                                        |                                                                                                                                                                                                                                                    TGTTGCTGAATTTGGGCTTTTGGGCAATTGCAATTGGCATTGCGATTTTTGTGGGACAAAACTCGCTGATTTCTTCCAACGCCATGGCGTCCATTTTAGAAAAATTCCCGAATGCCGCCTGCTCGGCAAATTCTATGGTCGGTAGTGTACGTTTCGGCACCGGCGCCTGCGTGGGA‑CACTGGTTGCCCTTATGAATATGAGCAGCGCAACGCCAATGTTGCTGACCATGGCGGCGTGTTCGTTGATGGCGGTGGTTTGCTATTATTTTTTAACCGCGCGCAATTTATAATTTTAAAAAGCGCATTATTGTAATCTGCAACGGACAGATAAATTTGTTCTAAATCAAAAATATCTCAAAGTAC  >  1/203617‑203978

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑