Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,474,262	T→C	T606A (ACA→GCA)	unknown ←	tRNA cytosine(34) acetyltransferase (EC 2.3.1.193)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,474,262	0	T	C	100.0%	214.0 / NA	76	T606A (ACA→GCA)	unknown	tRNA cytosine(34) acetyltransferase (EC 2.3.1.193)
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (46/30);  total (46/30)

CCTTGATCCACGCCTGCTTCTTTGCAGAAATCGGAATACGCATCCCAAGCGCGTTTTAACCAACCTTTTTTGGCTTCTTCAGCCATAGTTGCCTCCTTAGTCG‑TGCCGTTTTATTCTGGGTTGTTATCATAAGAAGGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTTTCAGCCACGTTTTTTTATGTTGAATGTCATCAGGGCGTTTGCAATAGGCTGTCAATAAAGGAAAATCCCGCTCCCCGCTTAATTTCATCAAGCGCCGAATAGCGGGCAAGGCAAACGCCAACGGCAGGAAGAAATCGGCAAAATCCGCCAAGCATTGCCAATCGGTTTCATTGAGCGACCAATCCGTTTCGGTTCCGACAAATTGCGTCGCCAGCGGGTGCAACGAAAGGGGCAGATTACGTTTAAATTGTTGCTCCGCCTGTTGCACCAACGCCCGTCCGCGCTCCGTCAGCGGGCA  >  1/1474029‑1474508                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                       ccTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGa                                                                                                                                                                                                                                              <  1:651044/245‑1 (MQ=255)     gATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATcctgtt                                                                                                                                                                                                                                    >  1:114500/1‑247 (MQ=255)          acaccggCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCg                                                                                                                                                                                                                               >  1:280371/7‑251 (MQ=255)          acaccggCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCg                                                                                                                                                                                                                               >  1:697933/7‑251 (MQ=255)                 cttcttTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTactt                                                                                                                                                                                                                        <  1:766491/251‑4 (MQ=255)                   tcttTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACttt                                                                                                                                                                                                                       >  1:674475/1‑250 (MQ=255)                        gCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAGGCTGCCAAt                                                                                                                                                                                                                                                    >  1:674247/1‑216 (MQ=255)                               tCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGa                                                                                                                                                                                                          >  1:730850/1‑251 (MQ=255)                                 ggAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAAt                                                                                                                                                                                                        >  1:25708/1‑251 (MQ=255)                                  gAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAg                                                                                                                                                                                                                                                >  1:155664/1‑210 (MQ=255)                                   aaTAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAg                                                                                                                                                                                                      <  1:243985/251‑1 (MQ=255)                                   aaTAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAg                                                                                                                                                                                                      <  1:474711/251‑1 (MQ=255)                                     taggCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACttt                                                                                                                                                                                                                       <  1:115330/229‑1 (MQ=255)                                           tCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCCTCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGggcaag                                                                                                                                                                                              >  1:435084/1‑251 (MQ=255)                                           tCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAgg                                                                                                                                                                                                                                               <  1:6763/202‑1 (MQ=255)                                           tCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGggcaag                                                                                                                                                                                              >  1:198214/1‑251 (MQ=255)                                           tCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGggcaag                                                                                                                                                                                              >  1:394941/1‑251 (MQ=255)                                             ccAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGggcaagac                                                                                                                                                                                            <  1:490986/251‑3 (MQ=255)                                                  cgcgTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACg                                                                                                                                                                                       <  1:716448/251‑1 (MQ=255)                                                    cgTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGc                                                                                                                                                                                      >  1:497927/1‑250 (MQ=255)                                                    cgTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTGTTTTATTTTGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATcctgttc                                                                                                                                                                                                                                   <  1:516088/205‑6 (MQ=255)                                                        ttAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCgg                                                                                                                                                                                                   >  1:543942/1‑233 (MQ=255)                                                         tAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACgg                                                                                                                                                                                <  1:848823/251‑1 (MQ=255)                                                               accTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGgaa                                                                                                                                                                          >  1:50446/1‑251 (MQ=255)                                                               accTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTACTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAAt                                                                                                                                                                                                        >  1:118271/1‑221 (MQ=255)                                                                ccTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGgaag                                                                                                                                                                         <  1:164548/251‑1 (MQ=255)                                                                   tttttAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGaaa                                                                                                                                                                      <  1:599641/251‑1 (MQ=255)                                                                             cttcAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGgaaga                                                                                                                                                                        >  1:809826/1‑239 (MQ=255)                                                                                  gtcagatttGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGc                                                                                                                                                        >  1:82166/8‑250 (MQ=255)                                                                                  gtcATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGc                                                                                                                                                        >  1:272769/3‑250 (MQ=255)                                                                                     atatttGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGc                                                                                                                                                                                                                              <  1:872792/173‑1 (MQ=255)                                                                                       atttGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAGGCTGCCATTAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCaaacc                                                                                                                                                  <  1:122563/249‑4 (MQ=255)                                                                                       atttGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCaaac                                                                                                                                                   >  1:261971/3‑248 (MQ=255)                                                                                               cctTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATcctgttc                                                                                                                                                                                                                                   >  1:242297/1‑157 (MQ=255)                                                                                               cctTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGccggt                                                                                                                                          >  1:26268/1‑248 (MQ=255)                                                                                                   agtcgatGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGc                                                                                                                                                                                      >  1:660204/7‑203 (MQ=255)                                                                                                           ccGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTg                                                                                                                               <  1:516656/250‑1 (MQ=255)                                                                                                             gTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAg                                                                                                                             >  1:152514/1‑250 (MQ=255)                                                                                                                  attttGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAAt                                                                                                                                                                                                        <  1:87641/166‑1 (MQ=255)                                                                                                                  attttGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCa                                                                                                                       >  1:332150/5‑251 (MQ=255)                                                                                                                            gttATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACggt                                                                                                                                                                               <  1:270628/185‑2 (MQ=255)                                                                                                                                                           cccTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCa                                                                              >  1:679235/1‑251 (MQ=255)                                                                                                                                                           cccTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCa                                                                              >  1:785990/1‑251 (MQ=255)                                                                                                                                                              tttCTTCACTTCCAACCGACAATTTGCCAGCCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCaatg                                                                           <  1:701517/251‑3 (MQ=255)                                                                                                                                                              tttCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCaatg                                                                           >  1:848411/1‑249 (MQ=255)                                                                                                                                                                 cttcACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGc                                                                               >  1:770914/1‑244 (MQ=255)                                                                                                                                                                 cttcACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGaaa                                                                        <  1:567562/251‑1 (MQ=255)                                                                                                                                                                     aCTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAgg                                                                      <  1:242533/249‑1 (MQ=255)                                                                                                                                                                      cTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAgggg                                                                    >  1:270092/1‑250 (MQ=255)                                                                                                                                                                      cTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGc                                                                   <  1:779751/251‑1 (MQ=255)                                                                                                                                                                                    tttgccaaccACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTaaa                                                     <  1:739232/243‑1 (MQ=255)                                                                                                                                                                                           accACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCggg                                                                                  >  1:58294/2‑215 (MQ=255)                                                                                                                                                                                               cGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGccgg                                                                                                                                           >  1:515173/1‑152 (MQ=255)                                                                                                                                                                                               cGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGc                                                                               >  1:284703/1‑214 (MQ=255)                                                                                                                                                                                                   tttttATTTTGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCaatga                                                                          >  1:593913/1‑212 (MQ=255)                                                                                                                                                                                                   tttttATTTTGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGcccg                                      >  1:298862/1‑249 (MQ=255)                                                                                                                                                                                                        attttGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCATTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCa                                 >  1:442306/4‑251 (MQ=255)                                                                                                                                                                                                          tttGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCAcc                               <  1:216956/250‑1 (MQ=255)                                                                                                                                                                                                          tttGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCAcc                               <  1:896113/250‑1 (MQ=255)                                                                                                                                                                                                            tGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCaa                             <  1:220851/251‑1 (MQ=255)                                                                                                                                                                                                                tGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGc                          >  1:806194/1‑250 (MQ=255)                                                                                                                                                                                                                  tcatcaGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCaa                             >  1:537666/1‑245 (MQ=255)                                                                                                                                                                                                                   catcaGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTg                                                                                               <  1:268539/178‑1 (MQ=255)                                                                                                                                                                                                                   catcaGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCg                       >  1:750548/1‑250 (MQ=255)                                                                                                                                                                                                                   catcaGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCAGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCg                       >  1:441831/1‑250 (MQ=255)                                                                                                                                                                                                                    atcaGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCgcc                     <  1:673041/251‑3 (MQ=255)                                                                                                                                                                                                                        ggacgcttGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGcccgcccgtg                 >  1:890464/7‑249 (MQ=255)                                                                                                                                                                                                                          acgcttGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGcccgcccgtgc                <  1:253026/246‑4 (MQ=255)                                                                                                                                                                                                                          acgcttGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGcccgcccgtgc                <  1:46631/246‑4 (MQ=255)                                                                                                                                                                                                                           cgcttGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCaatg                                                                           >  1:745488/4‑188 (MQ=255)                                                                                                                                                                                                                           cgcttGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGccc                                       <  1:463500/223‑2 (MQ=255)                                                                                                                                                                                                                             cttGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAg                                                                                                                             >  1:629123/2‑138 (MQ=255)                                                                                                                                                                                                                              ttGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGt           >  1:475604/1‑251 (MQ=255)                                                                                                                                                                                                                               tGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGtt          >  1:551660/1‑250 (MQ=255)                                                                                                                                                                                                                                gCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGtta         >  1:664681/1‑249 (MQ=255)                                                                                                                                                                                                                                       gCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGCAAATTGCGTTTAAATTGTTGTTCCGCCCGTTGCACCAACGCCCGCCCGTGCTCCGTTAGCggaca  >  1:410682/1‑248 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                       CCTTGATCCACGCCTGCTTCTTTGCAGAAATCGGAATACGCATCCCAAGCGCGTTTTAACCAACCTTTTTTGGCTTCTTCAGCCATAGTTGCCTCCTTAGTCG‑TGCCGTTTTATTCTGGGTTGTTATCATAAGAAGGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTTTCAGCCACGTTTTTTTATGTTGAATGTCATCAGGGCGTTTGCAATAGGCTGTCAATAAAGGAAAATCCCGCTCCCCGCTTAATTTCATCAAGCGCCGAATAGCGGGCAAGGCAAACGCCAACGGCAGGAAGAAATCGGCAAAATCCGCCAAGCATTGCCAATCGGTTTCATTGAGCGACCAATCCGTTTCGGTTCCGACAAATTGCGTCGCCAGCGGGTGCAACGAAAGGGGCAGATTACGTTTAAATTGTTGCTCCGCCTGTTGCACCAACGCCCGTCCGCGCTCCGTCAGCGGGCA  >  1/1474029‑1474508

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑