| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | 1 | 1,474,214 | G→A | L622L (CTG→TTG) | unknown ← | tRNA cytosine(34) acetyltransferase (EC 2.3.1.193) |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | 1 | 1,474,214 | 0 | G | A | 97.0% | 170.7 / NA | 66 | L622L (CTG→TTG) | unknown | tRNA cytosine(34) acetyltransferase (EC 2.3.1.193) |
| Reads supporting (aligned to +/- strand): ref base G (0/2); new base A (33/31); total (33/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.92e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTTCGGGATCTTCATCAAACTTCACCACCGGTACGCAGCCTCGGCAAGTGCCTTGATCCACGCCTGCTTCTTTGCAGAAATCGGAATACGCATCCCAAGCGCGTTTTAACCAACCTTTTTTGGCTTCTTCAGCCATAGTTGCCTCCTTAGTCG‑TGCCGTTTTATTCTGGGTTGTTATCATAAGAAGGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTTTCAGCCACGTTTTTTTATGTTGAATGTCATCAGGGCGTTTGCAATAGGCTGTCAATAAAGGAAAATCCCGCTCCCCGCTTAATTTCATCAAGCGCCGAATAGCGGGCAAGGCAAACGCCAACGGCAGGAAGAAATCGGCAAAATCCGCCAAGCATTGCCAATCGGTTTCATTGAGCGACCAATCCGTTTCGGTTCCGACAAATTGCGTCGCCAGCGGGTGCAACGAAAGGGGC > 1/1473979‑1474443 | gttcagGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTAt > 1:653584/1‑251 (MQ=255) ggATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGa > 1:830768/1‑251 (MQ=255) tcgaaCTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGtcatc < 1:792636/247‑1 (MQ=255) tcgaaCTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGtcatc < 1:589438/247‑1 (MQ=255) ccaccGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGc < 1:537030/251‑1 (MQ=255) ccGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAAt < 1:837236/251‑1 (MQ=255) ccGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAAt < 1:232882/251‑1 (MQ=255) gcaCGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAgg > 1:580848/3‑251 (MQ=255) gcaCGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAg < 1:765256/248‑1 (MQ=255) gcaCGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAg < 1:746828/248‑1 (MQ=255) caCGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAgg < 1:728463/249‑1 (MQ=255) gccccGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATg < 1:222198/218‑1 (MQ=255) ccTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGa < 1:651044/245‑1 (MQ=255) gATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAggac > 1:385174/1‑213 (MQ=255) gATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATcctgtt > 1:114500/1‑247 (MQ=255) acaccggCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCg > 1:697933/7‑251 (MQ=255) acaccggCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCg > 1:280371/7‑251 (MQ=255) cttcttTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTactt < 1:766491/251‑4 (MQ=255) tcttTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACttt > 1:674475/1‑250 (MQ=255) gCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAGGCTGCCAAt > 1:674247/1‑216 (MQ=255) tCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGa > 1:730850/1‑251 (MQ=255) ggAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAAt > 1:25708/1‑251 (MQ=255) gAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAg > 1:155664/1‑210 (MQ=255) aaTAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAg < 1:243985/251‑1 (MQ=255) aaTAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAg < 1:474711/251‑1 (MQ=255) taggCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACttt < 1:115330/229‑1 (MQ=255) ggctgcccAAGCGCGTTTTAGCCAACCTTTTTTAGCTTCTTCAGTCGTATTTGCCTCCTTAGTCGATGCCGTTTTATGTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTGCCAGCCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAGGc < 1:700849/189‑1 (MQ=255) tCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCCTCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGggcaag > 1:435084/1‑251 (MQ=255) tCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAgg < 1:6763/202‑1 (MQ=255) tCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGggcaag > 1:198214/1‑251 (MQ=255) tCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGggcaag > 1:394941/1‑251 (MQ=255) ccAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGggcaagac < 1:490986/251‑3 (MQ=255) cgcgTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACg < 1:716448/251‑1 (MQ=255) cgTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGc > 1:497927/1‑250 (MQ=255) cgTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTGTTTTATTTTGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATcctgttc < 1:516088/205‑6 (MQ=255) ttAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCgg > 1:543942/1‑233 (MQ=255) tAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACgg < 1:848823/251‑1 (MQ=255) accTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGgaa > 1:50446/1‑251 (MQ=255) accTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTACTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAAt > 1:118271/1‑221 (MQ=255) ccTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGgaag < 1:164548/251‑1 (MQ=255) tttttAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGaaa < 1:599641/251‑1 (MQ=255) cttcAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGgaaga > 1:809826/1‑239 (MQ=255) gtcagatttGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGc > 1:82166/8‑250 (MQ=255) gtcATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGc > 1:272769/3‑250 (MQ=255) atatttGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGc < 1:872792/173‑1 (MQ=255) atttGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAGGCTGCCATTAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCaaacc < 1:122563/249‑4 (MQ=255) atttGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCaaac > 1:261971/3‑248 (MQ=255) cctTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATcctgttc > 1:242297/1‑157 (MQ=255) cctTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGccggt > 1:26268/1‑248 (MQ=255) agtcgatGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGc > 1:660204/7‑203 (MQ=255) ccGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTg < 1:516656/250‑1 (MQ=255) gTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTAt < 1:801166/93‑1 (MQ=255) gTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAg > 1:152514/1‑250 (MQ=255) attttGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAAt < 1:87641/166‑1 (MQ=255) attttGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCa > 1:332150/5‑251 (MQ=255) gttATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACggt < 1:270628/185‑2 (MQ=255) tATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAGGc < 1:95856/107‑1 (MQ=255) cccTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCa > 1:785990/1‑251 (MQ=255) cccTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCa > 1:679235/1‑251 (MQ=255) tttCTTCACTTCCAACCGACAATTTGCCAGCCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCaatg < 1:701517/251‑3 (MQ=255) tttCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCaatg > 1:848411/1‑249 (MQ=255) cttcACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGc > 1:770914/1‑244 (MQ=255) cttcACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGaaa < 1:567562/251‑1 (MQ=255) aCTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAgg < 1:242533/249‑1 (MQ=255) cTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAgggg > 1:270092/1‑250 (MQ=255) cTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATCCTGTTCCCCGCTTACTTTCATCAAGCGCCGAATAGCGGGCAAGACAAACGCCAACGGTAGGAAGAAATCGGCAAAATCCGCCAAACCTTGCCGGTCGGCTTCATTGAGCGACCAATCCGTTTCGCTTCCGGCAAATTGTGTCGCCAGCGGGTGCAATGAAAGGGGc < 1:779751/251‑1 (MQ=255) | GTTCGGGATCTTCATCAAACTTCACCACCGGTACGCAGCCTCGGCAAGTGCCTTGATCCACGCCTGCTTCTTTGCAGAAATCGGAATACGCATCCCAAGCGCGTTTTAACCAACCTTTTTTGGCTTCTTCAGCCATAGTTGCCTCCTTAGTCG‑TGCCGTTTTATTCTGGGTTGTTATCATAAGAAGGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTTTCAGCCACGTTTTTTTATGTTGAATGTCATCAGGGCGTTTGCAATAGGCTGTCAATAAAGGAAAATCCCGCTCCCCGCTTAATTTCATCAAGCGCCGAATAGCGGGCAAGGCAAACGCCAACGGCAGGAAGAAATCGGCAAAATCCGCCAAGCATTGCCAATCGGTTTCATTGAGCGACCAATCCGTTTCGGTTCCGACAAATTGCGTCGCCAGCGGGTGCAACGAAAGGGGC > 1/1473979‑1474443 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |