Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,474,040	G→A	G25G (GGC→GGT)	unknown ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,474,040	0	G	A	100.0%	156.4 / NA	59	G25G (GGC→GGT)	unknown	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (37/22);  total (37/22)

GCTTAAAAATTGAGATTTCCCCCCCTCAGGGCTTCGCCCAGCTCCCCCGTGAACGAGGGGAACCAAGTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGTTGTGCCCCGGGTATCCCCGCTTAGTGCGTTTCTTTCTCCTCCGCCGTTTTTTCCTTCGGTTCGGGATCTTCATCAAACTTCACCACCGGTACGCAGCCTCGGCAAGTGCCTTGATCCACGCCTGCTTCTTTGCAGAAATCGGAATACGCATCCCAAGCGCGTTTTAACCAACCTTTTTTGGCTTCTTCAGCCATAGTTGCCTCCTTAGTCG‑TGCCGTTTTATTCTGGGTTGTTATCATAAGAAGGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTTTCAGCCACGTTTTTTTATGTTGAATGTCATCAGGGCGTTTGCAATAGGCTGTCAATAAAGGAAAATCCCGCT  >  1/1473813‑1474282                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                    gCTTAAAAATTTAGATTTTGCCCACTCAGGGCTTCGCCCAGCTCCCCCGTGAACGGGGGGAGCCAAGTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGa                                                                                                                                                                                                                              >  1:14479/1‑251 (MQ=255)                        acTCAGGGCTTCGCCCAGCTCCCCCGTGAACGGGGGGAGCCAAGTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCa                                                                                                                                                                                                                       <  1:329222/234‑1 (MQ=255)                        acTCAGGGCTTCGCCCAGCTCCCCCGTGAACGGGGGGAGCCAAGTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTa                                                                                                                                                                                                       >  1:692890/2‑251 (MQ=255)                        acTCAGGGCTTCGCCCAGCTCCCCCGTGAACGGGGGGAGCCAAGTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTa                                                                                                                                                                                                       >  1:590924/2‑251 (MQ=255)                        acTCAGGGCTTCGCCCAGCTCCCCCGTGAACGGGGGGAGCCAAGTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTa                                                                                                                                                                                                       >  1:532025/2‑251 (MQ=255)                        acTCAGGGCTTCGCCCAGCTCCCCCGTGAACGGGGGGAGCCAAGTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTa                                                                                                                                                                                                       >  1:494128/2‑251 (MQ=255)                                    gCCCAGCTCCCCCGTGAACGGGGGGAGCCAAGTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATccc                                                                                                                                                                                                                   >  1:36691/1‑227 (MQ=255)                                    gCCCAGCTCCCCCGTGAACGGGGGGAGCCAAGTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCttttt                                                                                                                                                                                           >  1:212320/1‑251 (MQ=255)                                          cTCCCCCGTGAACGGGGGGAGCCAAGTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGct                                                                                                                                                                                      <  1:764416/250‑1 (MQ=255)                                                          gggAGCCAAGTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTg                                                                                                                                                                      <  1:122096/250‑1 (MQ=255)                                                              gccAAGTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGcctc                                                                                                                                                                  >  1:151698/2‑250 (MQ=255)                                                               ccAAGTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGcctcc                                                                                                                                                                 <  1:497088/250‑1 (MQ=255)                                                                  aGTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAg                                                                                                                                                             >  1:243630/1‑251 (MQ=255)                                                                     tttttCTTTTTATATAAATAGTTACAATGTTTCGAACAGTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCg                                                                                                                                                          <  1:557630/251‑1 (MQ=255)                                                                                 tataAATAGTTACAATGTTTCGAACAGTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTAt                                                                                                                                              >  1:48770/1‑251 (MQ=255)                                                                                     aaTAGTTACAATGTTTCGAACAGTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTaac                                                                                                                                                                                                     >  1:741655/1‑192 (MQ=255)                                                                                     aaTAGTTACAATGTTTCGAACAGTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTAttttg                                                                                                                                          >  1:237814/1‑248 (MQ=255)                                                                                       tAGTTACAATGTTTCGAACAGTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATccc                                                                                                                                                                                                                   >  1:473958/1‑176 (MQ=255)                                                                                         gTTACAATGTTTCGAACAGTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTaac                                                                                                                                                                                                     <  1:169392/188‑1 (MQ=255)                                                                                         gTTACAATGTTTCGAACAGTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGgtt                                                                                                                                      >  1:338368/1‑251 (MQ=255)                                                                                               aTGTTTCGAACAGTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATc                                                                                                                                <  1:644077/251‑1 (MQ=255)                                                                                                 gTTTCGAACAGTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCAt                                                                                                                              >  1:124046/1‑251 (MQ=255)                                                                                                   ttCGAACAGTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGct                                                                                                                                                                                      >  1:20633/1‑193 (MQ=255)                                                                                                           gTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGcc                                                                                                                                                                    <  1:75581/203‑1 (MQ=255)                                                                                                           gTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATc                                                                                                                                <  1:323701/239‑1 (MQ=255)                                                                                                           gTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGa                                                                                                                    >  1:361764/1‑251 (MQ=255)                                                                                                           gTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGa                                                                                                                    >  1:486776/1‑251 (MQ=255)                                                                                                           gTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGa                                                                                                                    >  1:519951/1‑251 (MQ=255)                                                                                                              gtgCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAAt                                                                                                                 >  1:74588/1‑251 (MQ=255)                                                                                                                        gatctccGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGa                                                                                                                    >  1:456691/6‑238 (MQ=255)                                                                                                                        gatctccGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCaa                                                                                                       >  1:603870/6‑251 (MQ=255)                                                                                                                        gatctccGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCaa                                                                                                       >  1:283630/6‑251 (MQ=255)                                                                                                                          tctccGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATaagaa                                                                                                                         >  1:674734/4‑231 (MQ=255)                                                                                                                           ctccGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATattt                                                                                                                                                                       >  1:520510/3‑181 (MQ=255)                                                                                                                                    gTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATa                                                                                                                             >  1:517075/1‑217 (MQ=255)                                                                                                                                       cGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTc                                                                                        <  1:678144/251‑1 (MQ=255)                                                                                                                                        gTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTc                                                                                        >  1:301853/1‑250 (MQ=255)                                                                                                                                           tctttcttCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAgtca                                                                                                                                                                            >  1:27244/1‑160 (MQ=255)                                                                                                                                           tctttcttCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTAttt                                                                                                                                            >  1:67888/1‑194 (MQ=255)                                                                                                                                           tctttcttCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGgt                                                                                                                                       <  1:465414/200‑1 (MQ=255)                                                                                                                                                 ttctccGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACTAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACaa                                                                              <  1:689557/249‑1 (MQ=255)                                                                                                                                                        ccgTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAgtc                                                                                                                                                                             >  1:755964/1‑147 (MQ=255)                                                                                                                                                                       gttcagGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTAt                                                        >  1:653584/1‑251 (MQ=255)                                                                                                                                                                            ggATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGa                                                   >  1:830768/1‑251 (MQ=255)                                                                                                                                                                                     tcgaaCTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGtcatc                                           <  1:589438/247‑1 (MQ=255)                                                                                                                                                                                     tcgaaCTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGtcatc                                           <  1:792636/247‑1 (MQ=255)                                                                                                                                                                                               ccaccGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGc                                <  1:537030/251‑1 (MQ=255)                                                                                                                                                                                                  ccGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAAt                             <  1:837236/251‑1 (MQ=255)                                                                                                                                                                                                  ccGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAAt                             <  1:232882/251‑1 (MQ=255)                                                                                                                                                                                                     gcaCGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAgg                          >  1:580848/3‑251 (MQ=255)                                                                                                                                                                                                     gcaCGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAg                           <  1:746828/248‑1 (MQ=255)                                                                                                                                                                                                     gcaCGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAg                           <  1:765256/248‑1 (MQ=255)                                                                                                                                                                                                      caCGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAgg                          <  1:728463/249‑1 (MQ=255)                                                                                                                                                                                                            gccccGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATg                                                <  1:222198/218‑1 (MQ=255)                                                                                                                                                                                                                cGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGct                                                                                                                                                                                      >  1:441357/1‑84 (MQ=255)                                                                                                                                                                                                                         ccTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGa            <  1:651044/245‑1 (MQ=255)                                                                                                                                                                                                                             gATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTttcttc                                                                                             >  1:28530/1‑160 (MQ=255)                                                                                                                                                                                                                             gATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAggac                                      >  1:385174/1‑213 (MQ=255)                                                                                                                                                                                                                             gATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAATAGGCTGCCAATAAAGGAAAATcctgtt  >  1:114500/1‑247 (MQ=255)                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                    GCTTAAAAATTGAGATTTCCCCCCCTCAGGGCTTCGCCCAGCTCCCCCGTGAACGAGGGGAACCAAGTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGTTGTGCCCCGGGTATCCCCGCTTAGTGCGTTTCTTTCTCCTCCGCCGTTTTTTCCTTCGGTTCGGGATCTTCATCAAACTTCACCACCGGTACGCAGCCTCGGCAAGTGCCTTGATCCACGCCTGCTTCTTTGCAGAAATCGGAATACGCATCCCAAGCGCGTTTTAACCAACCTTTTTTGGCTTCTTCAGCCATAGTTGCCTCCTTAGTCG‑TGCCGTTTTATTCTGGGTTGTTATCATAAGAAGGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTTTCAGCCACGTTTTTTTATGTTGAATGTCATCAGGGCGTTTGCAATAGGCTGTCAATAAAGGAAAATCCCGCT  >  1/1473813‑1474282

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑