Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,474,010	T→C	V35V (GTA→GTG)	unknown ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,474,010	0	T	C	100.0%	175.3 / NA	64	V35V (GTA→GTG)	unknown	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (41/23);  total (41/23)

CCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTGAGATTTCCCCCCCTCAGGGCTTCGCCCAGCTCCCCCGTGAACGAGGGGAACCAAGTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGTTGTGCCCCGGGTATCCCCGCTTAGTGCGTTTCTTTCTCCTCCGCCGTTTTTTCCTTCGGTTCGGGATCTTCATCAAACTTCACCACCGGTACGCAGCCTCGGCAAGTGCCTTGATCCACGCCTGCTTCTTTGCAGAAATCGGAATACGCATCCCAAGCGCGTTTTAACCAACCTTTTTTGGCTTCTTCAGCCATAGTTGCCTCCTTAGTCG‑TGCCGTTTTATTCTGGGTTGTTATCATAAGAAGGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTTTCAGCCACGTTTTTTTATGTTGAATGTCATCAGGGCGTTTGCAAT  >  1/1473765‑1474255                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                       cccGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAGGGCTTCGCCCAGCTCCCCCGTGAACGGGGGGAGCCAAGTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGc                                                                                                                                                                                                                                                    <  1:270103/250‑1 (MQ=21)     aaGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAGGGCTTCGCCCAGCTCCCCCGTGAACGGGGGGAGCCAAGTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGcc                                                                                                                                                                                                                                                >  1:454825/1‑250 (MQ=21)       gggCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAGGGCTTCGCCCAGCTCCCCCGTGAACGGGGGGAGCCAAGTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGccccg                                                                                                                                                                                                                                             >  1:526883/1‑248 (MQ=21)        ggCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAGGGCTTCGCCCAGCTCCCCCGTGAACGGGGGGAGCCAAGTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCgg                                                                                                                                                                                                                                            >  1:233468/1‑251 (MQ=21)          cGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAGGGCTTCGCCCAGCTCCCCCGTGAACGGGGGGAGCCAAGTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCa                                                                                                                                                                                                                                          <  1:704721/251‑1 (MQ=21)             aaGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAGGGCTTCGCCCAGCTCCCCCGTGAACGGGGGGAGCCAAGTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAg                                                                                                                                                                                                                                        <  1:737887/250‑1 (MQ=255)                         aTCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAGGGCTTCGCCCAGCTCCCCCGTGAACGGGGGGAGCCAAGTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCa                                                                                                                                                                                                                            >  1:570497/1‑250 (MQ=255)                           cTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAGGGCTTCGCCCAGCTCCCCCGTGAACGGGGGGAGCCAAGTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCAcac                                                                                                                                                                                                                         <  1:365343/251‑3 (MQ=255)                                                 gCTTAAAAATTTAGATTTTGCCCACTCAGGGCTTCGCCCAGCTCCCCCGTGAACGGGGGGAGCCAAGTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGa                                                                                                                                                                                                   >  1:14479/1‑251 (MQ=255)                                                                   tgcccacTCAGGGCTTCGCCCAGCTCCCCCGTGAACGGGGGGAGCCAAGTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCAcac                                                                                                                                                                                                                         >  1:605292/7‑209 (MQ=255)                                                                        acTCAGGGCTTCGCCCAGCTCCCCCGTGAACGGGGGGAGCCAAGTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCGCCGGCACGCAGCCCCGGCAAGTGCCTTGATc                                                                                                                                                                                                                              >  1:441259/2‑201 (MQ=255)                                                                        acTCAGGGCTTCGCCCAGCTCCCCCGTGAACGGGGGGAGCCAAGTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCa                                                                                                                                                                                            <  1:329222/234‑1 (MQ=255)                                                                        acTCAGGGCTTCGCCCAGCTCCCCCGTGAACGGGGGGAGCCAAGTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTa                                                                                                                                                                            >  1:532025/2‑251 (MQ=255)                                                                        acTCAGGGCTTCGCCCAGCTCCCCCGTGAACGGGGGGAGCCAAGTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTa                                                                                                                                                                            >  1:494128/2‑251 (MQ=255)                                                                        acTCAGGGCTTCGCCCAGCTCCCCCGTGAACGGGGGGAGCCAAGTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTa                                                                                                                                                                            >  1:590924/2‑251 (MQ=255)                                                                        acTCAGGGCTTCGCCCAGCTCCCCCGTGAACGGGGGGAGCCAAGTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTa                                                                                                                                                                            >  1:692890/2‑251 (MQ=255)                                                                         cTCAGGGCTTCGCCCAGCTCCCCCGTGAACGGGGGGAGCCAAGTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGc                                                                                                                                                                                                                                                    <  1:370258/178‑1 (MQ=255)                                                                                    gCCCAGCTCCCCCGTGAACGGGGGGAGCCAAGTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATccc                                                                                                                                                                                        >  1:36691/1‑227 (MQ=255)                                                                                    gCCCAGCTCCCCCGTGAACGGGGGGAGCCAAGTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCttttt                                                                                                                                                                >  1:212320/1‑251 (MQ=255)                                                                                          cTCCCCCGTGAACGGGGGGAGCCAAGTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGct                                                                                                                                                           <  1:764416/250‑1 (MQ=255)                                                                                                          gggAGCCAAGTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTg                                                                                                                                           <  1:122096/250‑1 (MQ=255)                                                                                                              gccAAGTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGcctc                                                                                                                                       >  1:151698/2‑250 (MQ=255)                                                                                                               ccAAGTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGcctcc                                                                                                                                      <  1:497088/250‑1 (MQ=255)                                                                                                                  aGTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAg                                                                                                                                  >  1:243630/1‑251 (MQ=255)                                                                                                                     tttttCTTTTTATATAAATAGTTACAATGTTTCGAACAGTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCg                                                                                                                               <  1:557630/251‑1 (MQ=255)                                                                                                                                atataAATAGTTACAATGTTTCGAACAGTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCAc                                                                                                                                                                                                                           <  1:69407/148‑1 (MQ=255)                                                                                                                                 tataAATAGTTACAATGTTTCGAACAGTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTAt                                                                                                                   >  1:48770/1‑251 (MQ=255)                                                                                                                                     aaTAGTTACAATGTTTCGAACAGTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTaac                                                                                                                                                                          >  1:741655/1‑192 (MQ=255)                                                                                                                                     aaTAGTTACAATGTTTCGAACAGTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTAttttg                                                                                                               >  1:237814/1‑248 (MQ=255)                                                                                                                                       tAGTTACAATGTTTCGAACAGTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTg                                                                                                                                                                                                                                 >  1:323358/1‑135 (MQ=255)                                                                                                                                       tAGTTACAATGTTTCGAACAGTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATccc                                                                                                                                                                                        >  1:473958/1‑176 (MQ=255)                                                                                                                                         gTTACAATGTTTCGAACAGTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTaac                                                                                                                                                                          <  1:169392/188‑1 (MQ=255)                                                                                                                                         gTTACAATGTTTCGAACAGTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGgtt                                                                                                           >  1:338368/1‑251 (MQ=255)                                                                                                                                          ttACAATGTTTCGAACAGTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATc                                                                                                                                                                                                                              >  1:625627/1‑135 (MQ=255)                                                                                                                                          ttACAATGTTTCGAACAGTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCAcac                                                                                                                                                                                                                         >  1:680107/1‑138 (MQ=255)                                                                                                                                               aTGTTTCGAACAGTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATc                                                                                                     <  1:644077/251‑1 (MQ=255)                                                                                                                                                 gTTTCGAACAGTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCAt                                                                                                   >  1:124046/1‑251 (MQ=255)                                                                                                                                                   ttCGAACAGTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGct                                                                                                                                                           >  1:20633/1‑193 (MQ=255)                                                                                                                                                           gTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGcc                                                                                                                                         <  1:75581/203‑1 (MQ=255)                                                                                                                                                           gTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATc                                                                                                     <  1:323701/239‑1 (MQ=255)                                                                                                                                                           gTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGa                                                                                         >  1:519951/1‑251 (MQ=255)                                                                                                                                                           gTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGa                                                                                         >  1:486776/1‑251 (MQ=255)                                                                                                                                                           gTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGa                                                                                         >  1:361764/1‑251 (MQ=255)                                                                                                                                                              gtgCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAAt                                                                                      >  1:74588/1‑251 (MQ=255)                                                                                                                                                                        gatctccGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGa                                                                                         >  1:456691/6‑238 (MQ=255)                                                                                                                                                                        gatctccGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCaa                                                                            >  1:603870/6‑251 (MQ=255)                                                                                                                                                                        gatctccGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCaa                                                                            >  1:283630/6‑251 (MQ=255)                                                                                                                                                                          tctccGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATaagaa                                                                                              >  1:674734/4‑231 (MQ=255)                                                                                                                                                                           ctccGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATattt                                                                                                                                            >  1:520510/3‑181 (MQ=255)                                                                                                                                                                                    gTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATa                                                                                                  >  1:517075/1‑217 (MQ=255)                                                                                                                                                                                       cGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTc                                                             <  1:678144/251‑1 (MQ=255)                                                                                                                                                                                        gTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTc                                                             >  1:301853/1‑250 (MQ=255)                                                                                                                                                                                           tctttcttCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAgtca                                                                                                                                                 >  1:27244/1‑160 (MQ=255)                                                                                                                                                                                           tctttcttCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTAttt                                                                                                                 >  1:67888/1‑194 (MQ=255)                                                                                                                                                                                           tctttcttCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGgt                                                                                                            <  1:465414/200‑1 (MQ=255)                                                                                                                                                                                                 ttctccGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACTAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACaa                                                   <  1:689557/249‑1 (MQ=255)                                                                                                                                                                                                        ccgTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAgtc                                                                                                                                                  >  1:755964/1‑147 (MQ=255)                                                                                                                                                                                                                       gttcagGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTAt                             >  1:653584/1‑251 (MQ=255)                                                                                                                                                                                                                            ggATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGa                        >  1:830768/1‑251 (MQ=255)                                                                                                                                                                                                                                     tcgaaCTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGtcatc                <  1:589438/247‑1 (MQ=255)                                                                                                                                                                                                                                     tcgaaCTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGtcatc                <  1:792636/247‑1 (MQ=255)                                                                                                                                                                                                                                               ccaccGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGc     <  1:537030/251‑1 (MQ=255)                                                                                                                                                                                                                                                  ccGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAAt  <  1:232882/251‑1 (MQ=255)                                                                                                                                                                                                                                                  ccGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGAATGTCATCAGGACGCTTGCAAt  <  1:837236/251‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                       CCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTGAGATTTCCCCCCCTCAGGGCTTCGCCCAGCTCCCCCGTGAACGAGGGGAACCAAGTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGTTGTGCCCCGGGTATCCCCGCTTAGTGCGTTTCTTTCTCCTCCGCCGTTTTTTCCTTCGGTTCGGGATCTTCATCAAACTTCACCACCGGTACGCAGCCTCGGCAAGTGCCTTGATCCACGCCTGCTTCTTTGCAGAAATCGGAATACGCATCCCAAGCGCGTTTTAACCAACCTTTTTTGGCTTCTTCAGCCATAGTTGCCTCCTTAGTCG‑TGCCGTTTTATTCTGGGTTGTTATCATAAGAAGGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTTTCAGCCACGTTTTTTTATGTTGAATGTCATCAGGGCGTTTGCAAT  >  1/1473765‑1474255

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑