Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,473,995	A→G	F40F (TTT→TTC)	unknown ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,473,995	0	A	G	100.0%	167.9 / NA	62	F40F (TTT→TTC)	unknown	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (42/20);  total (42/20)

ATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTGAGATTTCCCCCCCTCAGGGCTTCGCCCAGCTCCCCCGTGAACGAGGGGAACCAAGTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGTTGTGCCCCGGGTATCCCCGCTTAGTGCGTTTCTTTCTCCTCCGCCGTTTTTTCCTTCGGTTCGGGATCTTCATCAAACTTCACCACCGGTACGCAGCCTCGGCAAGTGCCTTGATCCACGCCTGCTTCTTTGCAGAAATCGGAATACGCATCCCAAGCGCGTTTTAACCAACCTTTTTTGGCTTCTTCAGCCATAGTTGCCTCCTTAGTCG‑TGCCGTTTTATTCTGGGTTGTTATCATAAGAAGGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTTTCAGCCACGTTTTTTTATGTTGA  >  1/1473757‑1474233                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                aTAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAGGGCTTCGCCCAGCTCCCCCGTGAACGGGGGGAGCCAAGTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCaccacc                                                                                                                                                                                                                                     <  1:837115/251‑1 (MQ=18)    aCGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAGGGCTTCGCCCAGCTCCCCCGTGAACGGGGGGAGCCAAGTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGTTGTGCCCCAGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCgg                                                                                                                                                                                                                                   >  1:452456/1‑250 (MQ=18)     cGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAGGGCTTCGAACAGCTCCCCCGTGAACGGGGGGAGCCAAGTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTGTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCggca                                                                                                                                                                                                                                 <  1:424490/251‑3 (MQ=18)         cccGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAGGGCTTCGCCCAGCTCCCCCGTGAACGGGGGGAGCCAAGTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGc                                                                                                                                                                                                                              <  1:270103/250‑1 (MQ=21)             aaGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAGGGCTTCGCCCAGCTCCCCCGTGAACGGGGGGAGCCAAGTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGcc                                                                                                                                                                                                                          >  1:454825/1‑250 (MQ=21)               gggCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAGGGCTTCGCCCAGCTCCCCCGTGAACGGGGGGAGCCAAGTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGccccg                                                                                                                                                                                                                       >  1:526883/1‑248 (MQ=21)                ggCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAGGGCTTCGCCCAGCTCCCCCGTGAACGGGGGGAGCCAAGTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCgg                                                                                                                                                                                                                      >  1:233468/1‑251 (MQ=21)                  cGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAGGGCTTCGCCCAGCTCCCCCGTGAACGGGGGGAGCCAAGTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCa                                                                                                                                                                                                                    <  1:704721/251‑1 (MQ=21)                     aaGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAGGGCTTCGCCCAGCTCCCCCGTGAACGGGGGGAGCCAAGTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAg                                                                                                                                                                                                                  <  1:737887/250‑1 (MQ=255)                                 aTCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAGGGCTTCGCCCAGCTCCCCCGTGAACGGGGGGAGCCAAGTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCa                                                                                                                                                                                                      >  1:570497/1‑250 (MQ=255)                                   cTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAGGGCTTCGCCCAGCTCCCCCGTGAACGGGGGGAGCCAAGTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCAcac                                                                                                                                                                                                   <  1:365343/251‑3 (MQ=255)                                                         gCTTAAAAATTTAGATTTTGCCCACTCAGGGCTTCGCCCAGCTCCCCCGTGAACGGGGGGAGCCAAGTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGa                                                                                                                                                                             >  1:14479/1‑251 (MQ=255)                                                                           tgcccacTCAGGGCTTCGCCCAGCTCCCCCGTGAACGGGGGGAGCCAAGTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCAcac                                                                                                                                                                                                   >  1:605292/7‑209 (MQ=255)                                                                                acTCAGGGCTTCGCCCAGCTCCCCCGTGAACGGGGGGAGCCAAGTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCGCCGGCACGCAGCCCCGGCAAGTGCCTTGATc                                                                                                                                                                                                        >  1:441259/2‑201 (MQ=255)                                                                                acTCAGGGCTTCGCCCAGCTCCCCCGTGAACGGGGGGAGCCAAGTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCa                                                                                                                                                                      <  1:329222/234‑1 (MQ=255)                                                                                acTCAGGGCTTCGCCCAGCTCCCCCGTGAACGGGGGGAGCCAAGTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTa                                                                                                                                                      >  1:494128/2‑251 (MQ=255)                                                                                acTCAGGGCTTCGCCCAGCTCCCCCGTGAACGGGGGGAGCCAAGTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTa                                                                                                                                                      >  1:532025/2‑251 (MQ=255)                                                                                acTCAGGGCTTCGCCCAGCTCCCCCGTGAACGGGGGGAGCCAAGTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTa                                                                                                                                                      >  1:590924/2‑251 (MQ=255)                                                                                acTCAGGGCTTCGCCCAGCTCCCCCGTGAACGGGGGGAGCCAAGTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTa                                                                                                                                                      >  1:692890/2‑251 (MQ=255)                                                                                 cTCAGGGCTTCGCCCAGCTCCCCCGTGAACGGGGGGAGCCAAGTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGc                                                                                                                                                                                                                              <  1:370258/178‑1 (MQ=255)                                                                                            gCCCAGCTCCCCCGTGAACGGGGGGAGCCAAGTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATccc                                                                                                                                                                  >  1:36691/1‑227 (MQ=255)                                                                                            gCCCAGCTCCCCCGTGAACGGGGGGAGCCAAGTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCttttt                                                                                                                                          >  1:212320/1‑251 (MQ=255)                                                                                                  cTCCCCCGTGAACGGGGGGAGCCAAGTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGct                                                                                                                                     <  1:764416/250‑1 (MQ=255)                                                                                                                  gggAGCCAAGTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTg                                                                                                                     <  1:122096/250‑1 (MQ=255)                                                                                                                      gccAAGTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGcctc                                                                                                                 >  1:151698/2‑250 (MQ=255)                                                                                                                       ccAAGTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGcctcc                                                                                                                <  1:497088/250‑1 (MQ=255)                                                                                                                          aGTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAg                                                                                                            >  1:243630/1‑251 (MQ=255)                                                                                                                             tttttCTTTTTATATAAATAGTTACAATGTTTCGAACAGTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCg                                                                                                         <  1:557630/251‑1 (MQ=255)                                                                                                                                        atataAATAGTTACAATGTTTCGAACAGTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCAc                                                                                                                                                                                                     <  1:69407/148‑1 (MQ=255)                                                                                                                                         tataAATAGTTACAATGTTTCGAACAGTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTAt                                                                                             >  1:48770/1‑251 (MQ=255)                                                                                                                                             aaTAGTTACAATGTTTCGAACAGTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTaac                                                                                                                                                    >  1:741655/1‑192 (MQ=255)                                                                                                                                             aaTAGTTACAATGTTTCGAACAGTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTAttttg                                                                                         >  1:237814/1‑248 (MQ=255)                                                                                                                                               tAGTTACAATGTTTCGAACAGTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTg                                                                                                                                                                                                           >  1:323358/1‑135 (MQ=255)                                                                                                                                               tAGTTACAATGTTTCGAACAGTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATccc                                                                                                                                                                  >  1:473958/1‑176 (MQ=255)                                                                                                                                                 gTTACAATGTTTCGAACAGTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTaac                                                                                                                                                    <  1:169392/188‑1 (MQ=255)                                                                                                                                                 gTTACAATGTTTCGAACAGTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGgtt                                                                                     >  1:338368/1‑251 (MQ=255)                                                                                                                                                  ttACAATGTTTCGAACAGTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATc                                                                                                                                                                                                        >  1:625627/1‑135 (MQ=255)                                                                                                                                                  ttACAATGTTTCGAACAGTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCAcac                                                                                                                                                                                                   >  1:680107/1‑138 (MQ=255)                                                                                                                                                       aTGTTTCGAACAGTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATc                                                                               <  1:644077/251‑1 (MQ=255)                                                                                                                                                         gTTTCGAACAGTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCAt                                                                             >  1:124046/1‑251 (MQ=255)                                                                                                                                                           ttCGAACAGTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGct                                                                                                                                     >  1:20633/1‑193 (MQ=255)                                                                                                                                                                   gTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGcc                                                                                                                   <  1:75581/203‑1 (MQ=255)                                                                                                                                                                   gTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATc                                                                               <  1:323701/239‑1 (MQ=255)                                                                                                                                                                   gTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGa                                                                   >  1:486776/1‑251 (MQ=255)                                                                                                                                                                   gTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGa                                                                   >  1:361764/1‑251 (MQ=255)                                                                                                                                                                   gTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGa                                                                   >  1:519951/1‑251 (MQ=255)                                                                                                                                                                      gtgCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAAt                                                                >  1:74588/1‑251 (MQ=255)                                                                                                                                                                                gatctccGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGa                                                                   >  1:456691/6‑238 (MQ=255)                                                                                                                                                                                gatctccGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCaa                                                      >  1:603870/6‑251 (MQ=255)                                                                                                                                                                                gatctccGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCaa                                                      >  1:283630/6‑251 (MQ=255)                                                                                                                                                                                  tctccGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATaagaa                                                                        >  1:674734/4‑231 (MQ=255)                                                                                                                                                                                   ctccGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATattt                                                                                                                      >  1:520510/3‑181 (MQ=255)                                                                                                                                                                                            gTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATa                                                                            >  1:517075/1‑217 (MQ=255)                                                                                                                                                                                               cGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTc                                       <  1:678144/251‑1 (MQ=255)                                                                                                                                                                                                gTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTc                                       >  1:301853/1‑250 (MQ=255)                                                                                                                                                                                                   tctttcttCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAgtca                                                                                                                           >  1:27244/1‑160 (MQ=255)                                                                                                                                                                                                   tctttcttCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTAttt                                                                                           >  1:67888/1‑194 (MQ=255)                                                                                                                                                                                                   tctttcttCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGgt                                                                                      <  1:465414/200‑1 (MQ=255)                                                                                                                                                                                                         ttctccGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACTAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACaa                             <  1:689557/249‑1 (MQ=255)                                                                                                                                                                                                                ccgTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAgtc                                                                                                                            >  1:755964/1‑147 (MQ=255)                                                                                                                                                                                                                               gttcagGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTAt       >  1:653584/1‑251 (MQ=255)                                                                                                                                                                                                                                    ggATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGAATAGGCATCCCAAGCGCGTTTTAACCAACCTTTTTTAGCTTCTTCAGTCATATTTGCCTCCTTAGTCGATGCCGTTTTATTTTGGGTTGTTATCATAAGAAAGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTGCCAACCACGTTTTTTTATTTTGa  >  1:830768/1‑251 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                ATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTGAGATTTCCCCCCCTCAGGGCTTCGCCCAGCTCCCCCGTGAACGAGGGGAACCAAGTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGTTGTGCCCCGGGTATCCCCGCTTAGTGCGTTTCTTTCTCCTCCGCCGTTTTTTCCTTCGGTTCGGGATCTTCATCAAACTTCACCACCGGTACGCAGCCTCGGCAAGTGCCTTGATCCACGCCTGCTTCTTTGCAGAAATCGGAATACGCATCCCAAGCGCGTTTTAACCAACCTTTTTTGGCTTCTTCAGCCATAGTTGCCTCCTTAGTCG‑TGCCGTTTTATTCTGGGTTGTTATCATAAGAAGGCGAAATTTTTTTGCAACCCTTTCTTCACTTCCAACCGACAATTTTTCAGCCACGTTTTTTTATGTTGA  >  1/1473757‑1474233

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑