Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,473,836	C→A	intergenic (‑350/+105)	unknown ← / ← unknown	Transcription‑repair coupling factor/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,473,836	0	C	A	100.0%	132.7 / NA	63	intergenic (‑350/+105)	unknown/unknown	Transcription‑repair coupling factor/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (35/28);  total (35/28)

GCATTGCCTCGGGTATTACAGTTGTTCGAAACATTATAATTCATTGATATTTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCGTTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTGAGATTTCCCCCCCTCAGGGCTTCGCCCAGCTCCCCCGTGAACGAGGGGAACCAAGTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGTTGTGCCCCGGGTATCCCCGCTTAGTGCGTTTCTTTCTCCTCCGCCGTTTTTTCCTTCGGTTCGGGATCTTCATCAAACTTCACCACCGGTACGCAGCCTCGGCAAGTGCCTTGATCCACGCCTGCTTCTTTGCAGAAATCGGA  >  1/1473592‑1474063                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                    gCATTGCCCCGGGTATTACAGTTGTTCGAAACATTATAATTCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAggg                                                                                                                                                                                                                              >  1:705025/1‑251 (MQ=14) gCATTGCCCCGGGTATTACAGTTGTTCGAAACATTATAATTCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAggg                                                                                                                                                                                                                              >  1:50152/1‑251 (MQ=14) gCATTGCCCCGGGTATTACAGTTGTTCGAAACATTATAATTCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAggg                                                                                                                                                                                                                              >  1:845409/1‑251 (MQ=14)  cATTGCCCCGGGTATTACAGTTGTTCGAAACATTATAATTCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAGGGc                                                                                                                                                                                                                             <  1:368847/251‑1 (MQ=14)  cATTGCCCCGGGTATTACAGTTGTTCGAAACATTATAATTCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGAGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAggg                                                                                                                                                                                                                              <  1:126165/250‑1 (MQ=14)      gccccGGGTATTACAGTTGTTCGAAACATTATAATTCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAGGGCTTCg                                                                                                                                                                                                                         >  1:493436/5‑251 (MQ=14)           gggTATTACAGTTGTTCGAAACATTATAATTCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAGGGCTTCGCCCAg                                                                                                                                                                                                                    >  1:392023/1‑251 (MQ=255)             gTATTACAGTTGTTCGAAACATTATAATTCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAGGGCTTCGCCCAg                                                                                                                                                                                                                    >  1:558716/1‑249 (MQ=14)                 tACAGTTGTTCGAAACATTATAATTCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAGGGCTTCGCCCAGCTcccc                                                                                                                                                                                                              <  1:740927/251‑1 (MQ=255)                      ttgttCGAAACATTATAATTCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAGGGCTTCGCCCAGCTCCCCCGTGa                                                                                                                                                                                                         <  1:668103/251‑1 (MQ=255)                          tCGAAACATTATAATTCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAGGGCTTCGCCCAGCTCCCCCGTGAACgg                                                                                                                                                                                                     <  1:6479/251‑2 (MQ=14)                                cATTATAATTCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAGGGCTTCGCCCAGCTCCCCCGTGAACGGGGGGag                                                                                                                                                                                               >  1:261645/1‑250 (MQ=14)                                  ttATAATTCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAGGGc                                                                                                                                                                                                                             >  1:818221/1‑219 (MQ=11)                                  ttATAATTCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAGGGCTTCGCCCAGCTCCCCCGTGAAc                                                                                                                                                                                                       >  1:389946/1‑241 (MQ=14)                                      aaTTCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAGGGCTTCGCCCAGCTCCCCCGTGAACGGGGGGAGCCAAGt                                                                                                                                                                                         <  1:879846/251‑1 (MQ=14)                                        ttCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAGGGCTTCGCCCAGCTCCCCCGTGAACGGGGGGAGCCAAGttt                                                                                                                                                                                       >  1:62052/1‑251 (MQ=14)                                                    tttttAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAGGGCTTCGCCCAGCTCCCCCGTg                                                                                                                                                                                                          >  1:598963/1‑221 (MQ=255)                                                      tttAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAGGGCt                                                                                                                                                                                                                            >  1:329334/1‑201 (MQ=255)                                                      tttAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAGGGCTTCGCCCAGCTCCCCCGTGAACGGGGGGAGCCAAGTTTTTTCTTTTtatat                                                                                                                                                                          >  1:252/1‑251 (MQ=14)                                                                ttGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAGGGCTTCGCCCAGCTCCCCCGTGAACGGGGGGAGCCAAGTTTTTTCTTTTTATATAAATAGTTAc                                                                                                                                                                <  1:850765/251‑1 (MQ=14)                                                                ttGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAGGGCTTCGCCCAGCTCCCCCGTGAACGGGGGGAGCCAAGTTTTTTCTTTTTATATAAATAGTTAc                                                                                                                                                                <  1:507934/251‑1 (MQ=14)                                                                  gCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAGGGCTTCGCCCAGCTCCCCCGTGAAc                                                                                                                                                                                                       <  1:159695/210‑1 (MQ=255)                                                                  gCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAGGGCTTCGCCCAGCTCCCCCGTGAACGGGGGGAGCCAAGTTTTTTCTTTTTATATAAATAGTTACaa                                                                                                                                                              >  1:159791/1‑251 (MQ=14)                                                                  gCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAGGGCTTCGCCCAGCTCCCCCGTGAACGGGGGGAGCCAAGTTTTTTCTTTTTATATAAATAGTTACaa                                                                                                                                                              >  1:254037/1‑251 (MQ=14)                                                                  gCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAGGGCTTCGCCCAGCTCCCCCGTGAACGGGGGGAGCCAAGTTTTTTCTTTTTATATAAATAGTTACaa                                                                                                                                                              >  1:742567/1‑251 (MQ=14)                                                                  gCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAGGGCTTCGCCCAGCTCCCCCGTGAACGGGGGGAGCCAAGTTTTTTCTTTTTATATAAATAGTTACaa                                                                                                                                                              >  1:485354/1‑251 (MQ=14)                                                                       cctccGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAGGGCTTCGCCCAGCTCCCCCGTGAACGGGGGGAGCCAAGTTTTTTCTTTTTATATAAATAGTTACAATGttt                                                                                                                                                         >  1:492951/1‑251 (MQ=14)                                                                            gTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAGGGCTTCGCCCAGCTCCCCCGTGAAc                                                                                                                                                                                                       >  1:212281/1‑200 (MQ=14)                                                                            gTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAGGGCTTCGCCCAGCTCCCCCGTGAACGGGGGGAGCCAAGTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGaa                                                                                                                                                     >  1:415945/1‑250 (MQ=14)                                                                             tttACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAGGGCTTCGCCCAGCTCCCCCGTGAACGGGGGGAGCCAAGTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAAc                                                                                                                                                    <  1:711883/250‑1 (MQ=14)                                                                             tttACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAGGGCTTCGCCCAGCTCCCCCGTGAACGGGGGGAGCCAAGTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAAc                                                                                                                                                    <  1:596625/250‑1 (MQ=14)                                                                                 cGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAGGGCTTCGCCCAGCTCCCCCGTGAACGGGGGGAGCCAAGTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGTtg                                                                                                                                               <  1:225292/251‑1 (MQ=14)                                                                                       gACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAGGGCTTCGCCCAGCTCCCCCGTGAACGGGGGGAGCCAAGTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGTTGTGccc                                                                                                                                          <  1:789554/250‑1 (MQ=14)                                                                                            aaCGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAGGGCTTCGCCCAGCTCCCCCGTGAACGGGGGGAGCCAAGTTTTTTCTTTTTATATAAATAGTTACaa                                                                                                                                                              <  1:565987/225‑1 (MQ=14)                                                                                             aCGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGATTAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAGGGCTTCGCCCAGCACCCCCGTGAACGGGGGGAGACAAGTTTTTTCTTTTTATATCAATAGTTACATTGTTTCGAACaactgtaatacc                                                                                                                                        >  1:804899/1‑235 (MQ=14)                                                                                                       ttttGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAGGGCTTCGCCCAGCTCCCCCGTGAACGGGGGGAGCCAAGTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGTTGTGCCCCGGGGATCTCCGCTTAg                                                                                                                         <  1:901487/251‑1 (MQ=17)                                                                                                                  ggCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAGGGCTTCGCCCAGCTCCCCCGTGAACGGGGGGAGCCAAGTTTTTTCTTTTTATATAAATAg                                                                                                                                                                    <  1:840140/197‑1 (MQ=14)                                                                                                                  ggCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAGGGCTTCGCCCAGCTCCCCCGTGAACGGGGGGAGCCAAGTTTTTTCTTTTTATATAAATAGTTACAATg                                                                                                                                                            >  1:554798/1‑205 (MQ=14)                                                                                                                  ggCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAGGGCTTCGCCCAGCTCCCCCGTGAACGGGGGGAGCCAAGTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGTTGTGCCCCGGGGATCTCCGCTTAGTGCGtttcttt                                                                                                              >  1:159978/1‑251 (MQ=17)                                                                                                                               cccccAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAGGGCTTCGCCCAGCTCCCCCGTGAACGGGGGGAGCCAAGTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGtt                                                                                                 >  1:65322/1‑251 (MQ=17)                                                                                                                                        gggTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAGGGCTTCGCCCAGCTCCCCCGTGAACGGGGGGAGCCAAGTTTTTTCTTTTTATATAAATAGTTACAATg                                                                                                                                                            >  1:9202/1‑183 (MQ=14)                                                                                                                                          gTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAGGGCTTCGCCCAGCTCCCCCGTGAACGGGGGGAGCCAAGTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTttcgg                                                                                      >  1:109165/1‑251 (MQ=18)                                                                                                                                                 agtaagtTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAGGGCTTCGCCCAGCTCCCCCGTGAACGGGGGGAGCCAAGTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGa                                                                               >  1:55448/1‑251 (MQ=18)                                                                                                                                                      gtTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAGGGCTTCGCCCAGCTCCCCCGTGAACGGGGGGAGCCAAGTTTTTTCTTTTTATATAAATAGTTACAATg                                                                                                                                                            >  1:97420/1‑169 (MQ=14)                                                                                                                                                                 tGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAGGGCTTCGCCCAGCTCCCCCGTGAACGGGGGGAGCCAAGTTTTTTCTTTTTATATAAATAGTTACAATg                                                                                                                                                            <  1:394366/158‑1 (MQ=14)                                                                                                                                                                      aTAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAGGGCTTCGCCCAGCTCCCCCGTGAACGGGGGGAGCCAAGTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCaccacc                                                          <  1:837115/251‑1 (MQ=18)                                                                                                                                                                         aCGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAGGGCTTCGCCCAGCTCCCCCGTGAACGGGGGGAGCCAAGTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGTTGTGCCCCAGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCgg                                                        >  1:452456/1‑250 (MQ=18)                                                                                                                                                                          cGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAGGGCTTCGCCCAGCTCCCCCGTGAAc                                                                                                                                                                                                       <  1:295584/106‑1 (MQ=11)                                                                                                                                                                          cGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAGGGCTTCGAACAGCTCCCCCGTGAACGGGGGGAGCCAAGTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTGTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCggca                                                      <  1:424490/251‑3 (MQ=18)                                                                                                                                                                              cccGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAGGGCTTCGCCCAGCTCCCCCGTGAACGGGGGGAGCCAAGTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGc                                                   <  1:270103/250‑1 (MQ=21)                                                                                                                                                                                  aaGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAGGGCTTCGCCCAGCTCCCCCGTGAACGGGGGGAGCCAAGTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGcc                                               >  1:454825/1‑250 (MQ=21)                                                                                                                                                                                    gggCTGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAGGGCTTCGCCCAGCTCCCCCGTGAACGGGGGGAGCCAAGTTTTTTCTTTTTATATAAATAGTTACAATGt                                                                                                                                                           >  1:895663/1‑140 (MQ=14)                                                                                                                                                                                    gggCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAGGGCTTCGCCCAGCTCCCCCGTGAACGGGGGGAGCCAAGTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGccccg                                            >  1:526883/1‑248 (MQ=21)                                                                                                                                                                                     ggCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAGGGCTTCGCCCAGCTCCCCCGTGAACGGGGGGAGCCAAGTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCgg                                           >  1:233468/1‑251 (MQ=21)                                                                                                                                                                                       cGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAGGGCTTCGCCCAGCTCCCCCGTGAACGGGGGGAGCCAAGTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCa                                         <  1:704721/251‑1 (MQ=21)                                                                                                                                                                                          aaGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAGGGCTTCGCCCAGCTCCCCCGTGAACGGGGGGAGCCAAGTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAg                                       <  1:737887/250‑1 (MQ=255)                                                                                                                                                                                               gggATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAGGGCTTCGCCCAGCTCCCCCGTGAACGGGGGGAGCCAAGTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGTTGTGCCCCgggg                                                                                                                                     <  1:105131/151‑2 (MQ=255)                                                                                                                                                                                                      aTCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAGGGCTTCGCCCAGCTCCCCCGTGAACGGGGGGAGCCAAGTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGTTGTGccc                                                                                                                                          <  1:209461/139‑1 (MQ=255)                                                                                                                                                                                                      aTCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAGGGCTTCGCCCAGCTCCCCCGTGAACGGGGGGAGCCAAGTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGa                                                                               <  1:843753/198‑1 (MQ=255)                                                                                                                                                                                                      aTCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAGGGCTTCGCCCAGCTCCCCCGTGAACGGGGGGAGCCAAGTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGAt                                                                              <  1:762346/199‑1 (MQ=21)                                                                                                                                                                                                      aTCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAGGGCTTCGCCCAGCTCCCCCGTGAACGGGGGGAGCCAAGTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCa                           >  1:570497/1‑250 (MQ=255)                                                                                                                                                                                                        cTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAGGGCTTCGCCCAGCTCCCCCGTGAACGGGGGGAGCCAAGTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCAcac                        <  1:365343/251‑3 (MQ=255)                                                                                                                                                                                                                              gCTTAAAAATTTAGATTTTGCCCACTCAGGGCTTCGCCCAGCTCCCCCGTGAACGGGGGGAGCCAAGTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGTTGTGCCCCGGGGATCTCCGCTTAGTGCGTTTCTTTCTTCTCCGCCGTTTTTTCTTTCGGTTCAGGATCTTCGTCGAACTTCACCACCGGCACGCAGCCCCGGCAAGTGCCTTGATCCACACCGGCTTCTTTGCAGAAATCGGa  >  1:14479/1‑251 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                    GCATTGCCTCGGGTATTACAGTTGTTCGAAACATTATAATTCATTGATATTTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCGTTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTGAGATTTCCCCCCCTCAGGGCTTCGCCCAGCTCCCCCGTGAACGAGGGGAACCAAGTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGTTGTGCCCCGGGTATCCCCGCTTAGTGCGTTTCTTTCTCCTCCGCCGTTTTTTCCTTCGGTTCGGGATCTTCATCAAACTTCACCACCGGTACGCAGCCTCGGCAAGTGCCTTGATCCACGCCTGCTTCTTTGCAGAAATCGGA  >  1/1473592‑1474063

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑