Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,473,702	G→A	intergenic (‑216/+239)	unknown ← / ← unknown	Transcription‑repair coupling factor/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,473,702	0	G	A	100.0%	135.3 / NA	60	intergenic (‑216/+239)	unknown/unknown	Transcription‑repair coupling factor/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (31/29);  total (31/29)

TGAGTTTGCATAAAACGACAAAAGAGTAAGAAAAAAATTGCCGTTAGTTTAGCGCAAACGAGGGGGATTTCCAATAAAGATCTGTATAAAAAGACAGTAATTCTACCTAGGGCAAGGCATTGCCTCGGGTATTACAGTTGTTCGAAACATTATAATTCATTGATATTTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCGTTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTGAGATTTCCCCCCCTCAGGGCTTCGCCCAGCTCCCCCGTGAACGAGGGGAACCAAGTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGTTGTGCCCCGGGTATCCCCGCTTAG  >  1/1473476‑1473944                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                   tGAGTTTGCATAAAACGACAAAAGAGTAAGAAAAAAGTTGCAGTTAGTTTAGCGCAAACAAGAGGGATTTCCAATAAAGATCTGTATAAAAAGACAGTAATTCTACCTAGGGCAAAGCATTGCCCCGGGTATTACAGTTGTTCGAAACATTATAATTCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAgg                                                                                                                                                                                                                           <  1:598920/251‑1 (MQ=37)                         agTAAGAAAAAAGTTGCAGTTAGTTTAGCGCAAACAAGAGGGATTTCCAATAAAGATCTGTATAAAAAGACAGTAATTCTACCTAGGGCAAAGCATTGCCCCGGGTATTACAGTTGTTCGAAACATTATAATTCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGaa                                                                                                                                                                                                     <  1:123398/249‑1 (MQ=25)                         agTAAGAAAAAAGTTGCAGTTAGTTTAGCGCAAACAAGAGGGATTTCCAATAAAGATCTGTATAAAAAGACAGTAATTCTACCTAGGGCAAAGCATTGCCCCGGGTATTACAGTTGTTCGAAACATTATAATTCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAAc                                                                                                                                                                                                    <  1:454584/250‑1 (MQ=25)                                         cagTTAGTTTAGCGCAAACAAGAGGGATTTCCAATAAAGATCTGTATAAAAAGACAGTAATTCTACCTAGGGCAAAGCATTGCCCCGGGTATTACAGTTGTTCGAAACATTATAATTCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAgggg                                                                                                                                                                                                                         <  1:440748/211‑1 (MQ=25)                                               gTTTAGCGCAAACAAGAGGGATTTCCAATAAAGATCTGTATAAAAAGACAGTAATTCTACCTAGGGCAAAGCATTGCCCCGGGTATTACAGTTGTTCGAAACATTATAATTCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATaa                                                                                                                                                                                          >  1:754674/1‑238 (MQ=21)                                                                   aTTTCCAATAAAGATCTGTATAAAAAGACAGTAATTCTACCTAGGGCAAAGCATTGCCCCGGGTATTACAGTTGTTCGAAACATTATAATTCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCt                                                                                                                                                          <  1:357275/250‑1 (MQ=255)                                                                       ccAATAAAGATCTGTATAAAAAGACAGTAATTCTACCTAGGGCAAAGCATTGCCCCGGGTATTACAGTTGTTCGAAACATTATAATTCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAgg                                                                                                                                                                                                                           <  1:285552/181‑1 (MQ=255)                                                                        cAATAAAGATCTGTATAAAAAGACAGTAATTCTACCTAGGGCAAAGCATTGCCCCGGGTATTACAGTTGTTCGAAACATTATAATTCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGt                                                                                                                                                    >  1:807303/1‑251 (MQ=255)                                                                          aTAAAGATCTGTATAAAAAGACAGTAATTCTACCTAGGGCAAAGCATTGCCCCGGGTATTACAGTTGTTCGAAACATTATAATTCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGc                                                                                                                                                                                                                                               >  1:607509/1‑158 (MQ=25)                                                                                 tCTGTATAAAAAGACAGTAATTCTACCTAGGGCAAAGCATTGCCCCGGGTATTACAGTTGTTCGAAACATTATAATTCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCAATGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAg                                                                                                                                           <  1:686507/251‑1 (MQ=255)                                                                                    gTATAAAAAGACAGTAATTCTACCTAGGGCAAAGCATTGCCCCGGGTATTACAGTTGTTCGAAACATTATAATTCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGct                                                                                                                                                                                                                                              <  1:590411/149‑1 (MQ=21)                                                                                     tataAAAAGACAGTAATTCTACCTAGGGCAAAGCATTGCCCCGGGTATTACAGTTGTTCGAAACATTATAATTCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCt                                                                                                                                                          <  1:520546/232‑1 (MQ=255)                                                                                                  tAATTCTACCTAGGGCAAAGCATTGCCCCGGGTATTACAGTTGTTCGAAACATTATAATTCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAAttt                                                                                                                          >  1:621061/1‑250 (MQ=255)                                                                                                    aTTCTACCTAGGGCAAAGCATTGCCCCGGGTATTACAGTTGTTCGAAACATTATAATTCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATg                                                                                                                                                     <  1:551652/222‑1 (MQ=255)                                                                                                         aCCTAGGGCAAAGCATTGCCCCGGGTATTACAGTTGTTCGAAACATTATAATTCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAgg                                                                                                                                                                                                                           >  1:629812/1‑147 (MQ=18)                                                                                                         aCCTAGGGCAAAGCATTGCCCCGGGTATTACAGTTGTTCGAAACATTATAATTCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGAtttt                                                                                                                   >  1:679621/1‑250 (MQ=255)                                                                                                              gggCAAAGCATTGCCCCGGGTATTACAGTTGTTCGAAACATTATAATTCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGc                                                                                                                                                                                                                                               <  1:16384/122‑1 (MQ=18)                                                                                                              gggCAAAGCATTGCCCCGGGTATTACAGTTGTTCGAAACATTATAATTCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAAt                                                                                                                                                                                            <  1:540947/173‑1 (MQ=255)                                                                                                              gggCAAAGCATTGCCCCGGGTATTACAGTTGTTCGAAACATTATAATTCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGAttttgccca                                                                                                              >  1:824935/1‑245 (MQ=14)                                                                                                                     gCATTGCCCCGGGTATTACAGTTGTTCGAAACATTATAATTCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAggg                                                                                                       >  1:845409/1‑251 (MQ=14)                                                                                                                     gCATTGCCCCGGGTATTACAGTTGTTCGAAACATTATAATTCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAggg                                                                                                       >  1:705025/1‑251 (MQ=14)                                                                                                                     gCATTGCCCCGGGTATTACAGTTGTTCGAAACATTATAATTCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAggg                                                                                                       >  1:50152/1‑251 (MQ=14)                                                                                                                      cATTGCCCCGGGTATTACAGTTGTTCGAAACATTATAATTCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAGGGc                                                                                                      <  1:368847/251‑1 (MQ=14)                                                                                                                      cATTGCCCCGGGTATTACAGTTGTTCGAAACATTATAATTCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGAGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAggg                                                                                                       <  1:126165/250‑1 (MQ=14)                                                                                                                          gccccGGGTATTACAGTTGTTCGAAACATTATAATTCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAGGGCTTCg                                                                                                  >  1:493436/5‑251 (MQ=14)                                                                                                                               gggTATTACAGTTGTTCGAAACATTATAATTCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAGGGCTTCGCCCAg                                                                                             >  1:392023/1‑251 (MQ=255)                                                                                                                                 gTATTACAGTTGTTCGAAACATTATAATTCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAGGGCTTCGCCCAg                                                                                             >  1:558716/1‑249 (MQ=14)                                                                                                                                     tACAGTTGTTCGAAACATTATAATTCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAGGGCTTCGCCCAGCTcccc                                                                                       <  1:740927/251‑1 (MQ=255)                                                                                                                                          ttgttCGAAACATTATAATTCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAGGGCTTCGCCCAGCTCCCCCGTGa                                                                                  <  1:668103/251‑1 (MQ=255)                                                                                                                                            gttCGAAACATTATAATTCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATg                                                                                                                                                     >  1:694365/1‑182 (MQ=255)                                                                                                                                              tCGAAACATTATAATTCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAGGGCTTCGCCCAGCTCCCCCGTGAACgg                                                                              <  1:6479/251‑2 (MQ=14)                                                                                                                                                 aaaCATTATAATTCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCtt                                                                                                                                  <  1:87613/196‑1 (MQ=255)                                                                                                                                                    cATTATAATTCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAGGGCTTCGCCCAGCTCCCCCGTGAACGGGGGGag                                                                        >  1:261645/1‑250 (MQ=14)                                                                                                                                                      ttATAATTCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAGGGc                                                                                                      >  1:818221/1‑219 (MQ=11)                                                                                                                                                      ttATAATTCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAGGGCTTCGCCCAGCTCCCCCGTGAAc                                                                                >  1:389946/1‑241 (MQ=14)                                                                                                                                                          aaTTCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAGGGCTTCGCCCAGCTCCCCCGTGAACGGGGGGAGCCAAGt                                                                  <  1:879846/251‑1 (MQ=14)                                                                                                                                                            ttCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAGGGCTTCGCCCAGCTCCCCCGTGAACGGGGGGAGCCAAGttt                                                                >  1:62052/1‑251 (MQ=14)                                                                                                                                                                        tttttAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAGGGCTTCGCCCAGCTCCCCCGTg                                                                                   >  1:598963/1‑221 (MQ=255)                                                                                                                                                                          tttAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAGGGCt                                                                                                     >  1:329334/1‑201 (MQ=255)                                                                                                                                                                          tttAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAGGGCTTCGCCCAGCTCCCCCGTGAACGGGGGGAGCCAAGTTTTTTCTTTTtatat                                                   >  1:252/1‑251 (MQ=14)                                                                                                                                                                                 atCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGAttttgcc                                                                                                                >  1:770942/1‑179 (MQ=255)                                                                                                                                                                                 atCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGAt                                                                                                                      <  1:276756/177‑1 (MQ=255)                                                                                                                                                                                    ttGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAGGGCTTCGCCCAGCTCCCCCGTGAACGGGGGGAGCCAAGTTTTTTCTTTTTATATAAATAGTTAc                                         <  1:850765/251‑1 (MQ=14)                                                                                                                                                                                    ttGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAGGGCTTCGCCCAGCTCCCCCGTGAACGGGGGGAGCCAAGTTTTTTCTTTTTATATAAATAGTTAc                                         <  1:507934/251‑1 (MQ=14)                                                                                                                                                                                      gCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAGGGCTTCGCCCAGCTCCCCCGTGAAc                                                                                <  1:159695/210‑1 (MQ=255)                                                                                                                                                                                      gCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAGGGCTTCGCCCAGCTCCCCCGTGAACGGGGGGAGCCAAGTTTTTTCTTTTTATATAAATAGTTACaa                                       >  1:159791/1‑251 (MQ=14)                                                                                                                                                                                      gCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAGGGCTTCGCCCAGCTCCCCCGTGAACGGGGGGAGCCAAGTTTTTTCTTTTTATATAAATAGTTACaa                                       >  1:254037/1‑251 (MQ=14)                                                                                                                                                                                      gCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAGGGCTTCGCCCAGCTCCCCCGTGAACGGGGGGAGCCAAGTTTTTTCTTTTTATATAAATAGTTACaa                                       >  1:485354/1‑251 (MQ=14)                                                                                                                                                                                      gCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAGGGCTTCGCCCAGCTCCCCCGTGAACGGGGGGAGCCAAGTTTTTTCTTTTTATATAAATAGTTACaa                                       >  1:742567/1‑251 (MQ=14)                                                                                                                                                                                           cctccGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAGGGCTTCGCCCAGCTCCCCCGTGAACGGGGGGAGCCAAGTTTTTTCTTTTTATATAAATAGTTACAATGttt                                  >  1:492951/1‑251 (MQ=14)                                                                                                                                                                                                gTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCtt                                                                                                                                  >  1:569635/1‑150 (MQ=255)                                                                                                                                                                                                gTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAGGGCTTCGCCCAGCTCCCCCGTGAAc                                                                                >  1:212281/1‑200 (MQ=14)                                                                                                                                                                                                gTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAGGGCTTCGCCCAGCTCCCCCGTGAACGGGGGGAGCCAAGTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGaa                              >  1:415945/1‑250 (MQ=14)                                                                                                                                                                                                 tttACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAGGGCTTCGCCCAGCTCCCCCGTGAACGGGGGGAGCCAAGTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAAc                             <  1:711883/250‑1 (MQ=14)                                                                                                                                                                                                 tttACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAGGGCTTCGCCCAGCTCCCCCGTGAACGGGGGGAGCCAAGTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAAc                             <  1:596625/250‑1 (MQ=14)                                                                                                                                                                                                     cGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAGGGCTTCGCCCAGCTCCCCCGTGAACGGGGGGAGCCAAGTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGTtg                        <  1:225292/251‑1 (MQ=14)                                                                                                                                                                                                           gACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAGGGCTTCGCCCAGCTCCCCCGTGAACGGGGGGAGCCAAGTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGTTGTGccc                   <  1:789554/250‑1 (MQ=14)                                                                                                                                                                                                                aaCGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAGGGCTTCGCCCAGCTCCCCCGTGAACGGGGGGAGCCAAGTTTTTTCTTTTTATATAAATAGTTACaa                                       <  1:565987/225‑1 (MQ=14)                                                                                                                                                                                                                 aCGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGATTAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAGGGCTTCGCCCAGCACCCCCGTGAACGGGGGGAGACAAGTTTTTTCTTTTTATATCAATAGTTACATTGTTTCGAACaactgtaatacc                 >  1:804899/1‑235 (MQ=14)                                                                                                                                                                                                                           ttttGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAGGGCTTCGCCCAGCTCCCCCGTGAACGGGGGGAGCCAAGTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGTTGTGCCCCGGGGATCTCCGCTTAg  <  1:901487/251‑1 (MQ=17)                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                   TGAGTTTGCATAAAACGACAAAAGAGTAAGAAAAAAATTGCCGTTAGTTTAGCGCAAACGAGGGGGATTTCCAATAAAGATCTGTATAAAAAGACAGTAATTCTACCTAGGGCAAGGCATTGCCTCGGGTATTACAGTTGTTCGAAACATTATAATTCATTGATATTTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCGTTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTGAGATTTCCCCCCCTCAGGGCTTCGCCCAGCTCCCCCGTGAACGAGGGGAACCAAGTTTTTTCTTTTTATATAAATAGTTACAATGTTTCGAACAGTTGTGCCCCGGGTATCCCCGCTTAG  >  1/1473476‑1473944

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑