Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,473,600	T→C	intergenic (‑114/+341)	unknown ← / ← unknown	Transcription‑repair coupling factor/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,473,600	0	T	C	100.0%	139.5 / NA	55	intergenic (‑114/+341)	unknown/unknown	Transcription‑repair coupling factor/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (26/29);  total (26/29)

CACGGTTAGACCGTCAAACTGCGCGGCAATTTCGCTAATGGCGAGGCTATCCGCACCGGGCAGGATATTGGCTAAAATTTTATGATCATTCGCTTGGGTAGGAATTTCGAGATGAAAAAATTGAGTTTGCATAAAACGACAAAAGAGTAAGAAAAAAATTGCCGTTAGTTTAGCGCAAACGAGGGGGATTTCCAATAAAGATCTGTATAAAAAGACAGTAATTCTACCTAGGGCAAGGCATTGCCTCGGGTATTACAGTTGTTCGAAACATTATAATTCATTGATATTTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCGTTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTGAGATTTCCCCCCCTCAGGGC  >  1/1473355‑1473844                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                     cacGGTTAAACCATCAAACTGCGCGGCAATTTCGCTAATGGCGAGGCTATCCGCGCCGGGCAGGATATTGGCTAAAATTTTATGATCATTCACTTGGGTAGGAATTTCGAGATGAAAAAATTGAGTTTGCATAAAACGACAAAAGAGTAAGAAAAAAGTTGCAGTTAGTTTAGCGCAAACAAGAGGGATTTCCAATAAAGATCTGTATAAAAAGACAGTAATTCTACCTAGGGCAAAGCATTGCCCCGGGt                                                                                                                                                                                                                                                 <  1:121391/251‑1 (MQ=255)   cGGTTAAACCATCAAACTGCGCGGCAATTTCGCTAATGGCGAGGCTATCCGCGCCGGGCAGGATATTGGCTAAAATTTTATGATCATTCACTTGGGTAGGAATTTCGAGATGAAAAAATTGAGTTTGCATAAAACGACAAAAGAGTAAGAAAAAAGTTGCAGTTAGTTTAGCGCAAACAAGAGGGATTTCCAATAAAGATCTGTATAAAAAGACAGTAATTCTACCTAGGGCAAAGCATTGCCCCGGGTAt                                                                                                                                                                                                                                               >  1:628819/1‑251 (MQ=255)      ttaaaccatCAAACTGCGCGGCAATTTCGCTAATGGCGAGGCTATCCGCGCCGGGCAGGATATTGGCTAAAATTTTATGATCATTCACTTGGGTAGGAATTTCGAGATGAAAAAATTGAGTTTGCATAAAACGACAAAAGAGTAAGAAAAAAGTTGCAGTTAGTTTAGCGCAAACAAGAGGGATTTCCAATAAAGATCTGTATAAAAAGACAGTAATTCTACCTAGGGCAAAGCATTGCCCCGGGTATTAc                                                                                                                                                                                                                                            <  1:500241/243‑1 (MQ=255)      ttaaaccatCAAACTGCGCGGCAATTTCGCTAATGGCGAGGCTATCCGCGCCGGGCAGGATATTGGCTAAAATTTTATGATCATTCACTTGGGTAGGAATTTCGAGATGAAAAAATTGAGTTTGCATAAAACGACAAAAGAGTAAGAAAAAAGTTGCAGTTAGTTTAGCGCAAACAAGAGGGATTTCCAATAAAGATCTGTATAAAAAGACAGTAATTCTACCTAGGGCAAAGCATTGCCCCGGGTATTAc                                                                                                                                                                                                                                            <  1:14436/243‑1 (MQ=255)        aaaccatCAAACTGCGCGGCAATTTCGCTAATGGCGAGGCTATCCGCGCCGGGCAGGATATTGGCTAAAATTTTATGATCATTCACTTGGGTAGGAATTTCGAGATGAAAAAATTGAGTTTGCATAAAACGACAAAAGAGTAAGAAAAAAGTTGCAGTTAGTTTAGCGCAAACAAGAGGGATTTCCAATAAAGATCTGTATAAAAAGACAGTAATTCTACCTAGGGCAAAGCATTGCCCCGGGTATTACAg                                                                                                                                                                                                                                          <  1:317448/245‑1 (MQ=255)        aaaccatCAAACTGCGCGGCAATTTCGCTAATGGCGAGGCTATCCGCGCCGGGCAGGATATTGGCTAAAATTTTATGATCATTCACTTGGGTAGGAATTTCGAGATGAAAAAATTGAGTTTGCATAAAACGACAAAAGAGTAAGAAAAAAGTTGCAGTTAGTTTAGCGCAAACAAGAGGGATTTCCAATAAAGATCTGTATAAAAAGACAGTAATTCTACCTAGGGCAAAGCATTGCCCCGGGTATTACAg                                                                                                                                                                                                                                          <  1:364516/245‑1 (MQ=255)           ccatCAAACTGCGCGGCAATTTCGCTAATGGCGAGGCTATTCGCGCCGGGCAGGATATTGGCTAAAATTTTATGATCATTCACTTGGGTAAGAATTTCGAGATGAAAAAATTGAGGTTGCATAAAACGACAAAAGAGTAAGAAAAAAGTTGCAGTTAGTTTAGCGCAAACAAGAGGGATTTCCAATAAAGATCTGTATAAAAAGACAGTAATTCTACCTAGGGCAAAGCATTGCCCCGGGTATTACAgttg                                                                                                                                                                                                                                       <  1:435030/248‑1 (MQ=255)                                 cGCTAATGGCGAGGCTATCCGCGCCGGGCAGGATATTGGCTAAAATTTTATGATCATTCACTTGGGTAGGAATTTCGAGATGAAAAAATTGAGTTTGCATAAAACGACAAAAGAGTAAGAAAAAAGTTGCAGTTAGTTTAGCGCAAACAAGAGGGATTTCCAATAAAGATCTGTATAAAAAGACAGTAATTCTACCTAGGGCAAAGCATTGCCCCGGGTATTACAGTTGTTCGAAACATTATAAt                                                                                                                                                                                                                       >  1:753180/1‑245 (MQ=255)                                 cGCTAATGGCGAGGCTATCCGCGCCGGGCAGGATATTGGCTAAAATTTTATGATCATTCACTTGGGTAGGAATTTCGAGATGAAAAAATTGAGTTTGCATAAAACGACAAAAGAGTAAGAAAAAAGTTGCAGTTAGTTTAGCGCAAACAAGAGGGATTTCCAATAAAGATCTGTATAAAAAGACAGTAATTCTACCTAGGGCAAAGCATTGCCCCGGGTATTACAGTTGTTCGAAACATTATAATTCATTg                                                                                                                                                                                                                 >  1:292915/1‑251 (MQ=255)                                   cTAATGGCGAGGCTATCCGCGCCGGGCAGGATATTGGCTAAAATTTTATGATCATTCACTTGGGTAGGAATTTCGAGATGAAAAAATTGAGTTTGCATAAAACGACAAAAGAGTAAGAAAAAAGTTGCAGTTAGTTTAGCGCAAACAAGAGGGATTTCCAATAAAGATCTGTATAAAAAGACAGTAATTCTACCTAGGGCAAAGCATTGCCCCGGGTATTACAGTTGTTCGAAACATTATAATTCATTGat                                                                                                                                                                                                               <  1:108388/251‑1 (MQ=255)                                       tGGCGAGGCTATCCGCGCCGGGCAGGATATTGGCTAAAATTTTATGATCATTCACTTGGGTAGGAATTTCGAGATGAAAAAATTGAGTTTGCATAAAACGACAAAAGAGTAAGAAAAAAGTTGCAGTTAGTTTAGCGCAAACAAGAGGGATTTCCAATAAAGATCTGTATAAAAAGACAGTAATTCTACCTAGGGCAAAGCATTGCCCCGGGTATTACAGTTGTTCGAAACATTATAAt                                                                                                                                                                                                                       >  1:241638/1‑239 (MQ=255)                                              gCTATCCGCGCCGGGCAGGATATTGGCTAAAATTTTATGATCATTCACTTGGGTAGGAATTTCGAGATGAAAAAATTGAGTTTGCATAAAACGACAAAAGAGTAAGAAAAAAGTTGCAGTTAGTTTAGCGCAAACAAGAGGGATTTCCAATAAAGATCTGTATAAAAAGACAGTAATTCTACCTAGGGCAAAGCATTGCCCCGGGTATTACAGTTGTTCGAAACATtat                                                                                                                                                                                                                          >  1:241559/1‑229 (MQ=255)                                              gCTATCCGCGCCGGGCAGGATATTGGCTAAAATTTTATGATCATTCACTTGGGTAGGAATTTCGAGATGAAAAAATTGAGTTTGCATAAAACGACAAAAGAGTAAGAAAAAAGTTGCAGTTAGTTTAGCGCAAACAAGAGGGATTTCCAATAAAGATCTGTATAAAAAGACAGTAATTCTACCTAGGGCAAAGCATTGCCCCGGGTATTACAGTTGTTCGAAACATTATAATTCATTGATAttttttaaat                                                                                                                                                                                                    >  1:361648/1‑247 (MQ=255)                                                 aTCCGCGCCGGGCAGGATATTGGCTAAAATTTTATGATCATTCACTTGGGTAGGAATTTCGAGATGAAAAAATTGAGTTTGCATAAAACGACAAAAGAGTAAGAAAAAAGTTGCAGTTAGTTTAGCGCAAACAAGAGGGATTTCCAATAAAGATCTGTATAAAAAGACAGTAATTCTACCTAGGGCAAAGCATTGCCCCGGGTATTAc                                                                                                                                                                                                                                            >  1:564625/1‑208 (MQ=255)                                                          gggCAGGATATTGGCTAAAATTTTATGATCATTCACTTGGGTAGGAATTTCGAGATGAAAAAATTGAGTTTGCATAAAACGACAAAAGAGTAAGAAAAAAGTTGCAGTTAGTTTAGCGCAAACAAGAGGGATTTCCAATAAAGATCTGTATAAAAAGACAGTAATTCTACCTAGGGCAAAGCATTGCCCCGGGTATTACAGTTGTTCGAAACATTATAAt                                                                                                                                                                                                                       <  1:749444/220‑1 (MQ=255)                                                               ggATATTGGCTAAAATTTTATGATCATTCACTTGGGTAGGAATTTCGAGATGAAAAAATTGAGTTTGCATAAAACGACAAAAGAGTAAGAAAAAAGTTGCAGTTAGTTTAGCGCAAACAAGAGGGATTTCCAATAAAGATCTGTATAAAAAGACAGTAATTCTACCTAGGGCAAAGCATTGCCCCGGGTATTACAGTTGTTCGAAACATTATAATTCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGt                                                                                                                                                                                  >  1:849332/1‑251 (MQ=255)                                                                gATATTGGCTAAAATTTTATGATCATTCACTTGGGTAGGAATTTCGAGATGAAAAAATTGAGTTTGCATAAAACGACAAAAGAGTAAGAAAAAAGTTGCAGTTAGTTTAGCGCAAACAAGAGGGATTTCCAATAAAGATCTGTATAAAAAGACAGTAATTCTACCTAGGGCAAAGCATTGCCCCGGGTATTAc                                                                                                                                                                                                                                            >  1:296127/1‑193 (MQ=255)                                                                       gCTAAAATTTTATGATCATTCACTTGGGTAGGAATTTCGAGATGAAAAAATTGAGTTTGCATAAAACGACAAAAGAGTAAGAAAAAAGTTGCAGTTAGTTTAGCGCAAACAAGAGGGATTTCCAATAAAGATCTGTATAAAAAGACAGTAATTCTACCTAGGGCAAAGCATTGCCCCGGGTATTACAGTTGTTCGAAACATTATAATTCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGTTTACggg                                                                                                                                                                           <  1:790940/250‑1 (MQ=255)                                                                            aaTTTTATGATCATTCACTTGGGTAGGAATTTCGAGATGAAAAAATTGAGTTTGCATAAAACGACAAAAGAGTAAGAAAAAAGTTGCAGTTAGTTTAGCGCAAACAAGAGGGATTTCCAATAAAGATCTGTATAAAAAGACAGTAATTCTACCTAGGGCAAAGCATTGCCCCGGGTATTACAGTTGTTCGAAACATTATAATTCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACa                                                                                                                                                                     >  1:793962/1‑251 (MQ=39)                                                                                     aTCATTCACTTGGGTAGGAATTTCGAGATGAAAAAATTGAGTTTGCATAAAACGACAAAAGAGTAAGAAAAAAGTTGCAGTTAGTTTAGCGCAAACAAGAGGGATTTCCAATAAAGATCTGTATAAAAAGACAGTAATTCTACCTAGGGCAAAGCATTGCCCCGGGTATTACAGTTGTTCGAAACATTATAATTCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGTTTACggg                                                                                                                                                                           <  1:616048/236‑1 (MQ=39)                                                                                           cacTTGGGTAGGAATTTCGAGATGAAAAAATTGAGTTTGCATAAAACGACAAAAGAGTAAGAAAAAAGTTGCAGTTAGTTTAGCGCAAACAAGAGGGATTTCCAATAAAGATCTGTATAAAAAGACAGTAATTCTACCTAGGGCAAAGCATTGCCCCGGGTATTACAGTTGTTCGAAACATTATAATTCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGttt                                                                                                                                                      >  1:94864/3‑251 (MQ=38)                                                                                                gggTAGGAATTTCGAGATGAAAAAATTGAGTTTGCATAAAACGACAAAAGAGTAAGAAAAAAGTTGCAGTTAGTTTAGCGCAAACAAGAGGGATTTCCAATAAAGATCTGTATAAAAAGACAGTAATTCTACCTAGGGCAAAGCATTGCCCCGGGTATTAc                                                                                                                                                                                                                                            <  1:822983/161‑1 (MQ=255)                                                                                                 ggTAGGAATTTCGAGATGAAAAAATTGAGTTTGCATAAAACGACAAAAGAGTAAGAAAAAAGTTGCAGTTAGTTTAGCGCAAACAAGAGGGATTTCCAATAAAGATCTGTATAAAAAGACAGTAATTCTACCTAGGGCAAAGCATTGCCCCGGGt                                                                                                                                                                                                                                                 <  1:749997/155‑1 (MQ=255)                                                                                                                     aaaaTTGAGTTTGCATAAAACGACAAAAGAGTAAGAAAAAAGTTGCAGTTAGTTTAGCGCAAACAAGAGGGATTTCCAATAAAGATCTGTATAAAAAGACAGTAATTCTACCTAGGGCAAAGCATTGCCCCGGGTATTAc                                                                                                                                                                                                                                            <  1:772607/140‑1 (MQ=255)                                                                                                                       aaTTGAGTTTGCATAAAACGACAAAAGAGTAAGAAAAAAGTTGCAGTTAGTTTAGCGCAAACAAGAGGGATTTCCAATAAAGATCTGTATAAAAAGACAGTAATTCTACCTAGGGCAAAGCATTGCCCCGGGTATTACAGTTGTTCGAAACATTATAATTc                                                                                                                                                                                                                     >  1:583595/1‑161 (MQ=255)                                                                                                                          tGAGTTTGCATAAAACGACAAAAGAGTAAGAAAAAAGTTGCAGTTAGTTTAGCGCAAACAAGAGGGATTTCCAATAAAGATCTGTATAAAAAGACAGTAATTCTACCTAGGGCAAAGCATTGCCCCGGGTATTACAGTTGTTCGAAACATTATAATTCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAgg                                                                                                                       <  1:598920/251‑1 (MQ=37)                                                                                                                                           aCAAAAGAGTAAGAAAAAAGTTGCAGTTAGTTTAGCGCAAACAAGAGGGATTTCCAATAAAGATCTGTATAAAAAGACAGTAATTCTACCTAGGGCAAAGCATTGCCCCGGGTATTACAGTTGTTCGAAACATTATAATTc                                                                                                                                                                                                                     >  1:707718/1‑141 (MQ=255)                                                                                                                                                  agTAAGAAAAAAGTTGCAGTTAGTTTAGCGCAAACAAGAGGGATTTCCAATAAAGATCTGTATAAAAAGACAGTAATTCTACCTAGGGCAAAGCATTGCCCCGGGTATTACAGTTGTTCGAAACATTATAATTCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGaa                                                                                                 <  1:123398/249‑1 (MQ=25)                                                                                                                                                  agTAAGAAAAAAGTTGCAGTTAGTTTAGCGCAAACAAGAGGGATTTCCAATAAAGATCTGTATAAAAAGACAGTAATTCTACCTAGGGCAAAGCATTGCCCCGGGTATTACAGTTGTTCGAAACATTATAATTCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAAc                                                                                                <  1:454584/250‑1 (MQ=25)                                                                                                                                                                  cagTTAGTTTAGCGCAAACAAGAGGGATTTCCAATAAAGATCTGTATAAAAAGACAGTAATTCTACCTAGGGCAAAGCATTGCCCCGGGTATTACAGTTGTTCGAAACATTATAATTCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAgggg                                                                                                                     <  1:440748/211‑1 (MQ=25)                                                                                                                                                                      tAGTTTAGCGCAAACAAGAGGGATTTCCAATAAAGATCTGTATAAAAAGACAGTAATTCTACCTAGGGCAAAGCATTGCCCCGGGTATTAc                                                                                                                                                                                                                                            >  1:526782/1‑91 (MQ=255)                                                                                                                                                                        gTTTAGCGCAAACAAGAGGGATTTCCAATAAAGATCTGTATAAAAAGACAGTAATTCTACCTAGGGCAAAGCATTGCCCCGGGTATTACAGTTGTTCGAAACATTATAATTCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATaa                                                                                      >  1:754674/1‑238 (MQ=21)                                                                                                                                                                                            aTTTCCAATAAAGATCTGTATAAAAAGACAGTAATTCTACCTAGGGCAAAGCATTGCCCCGGGTATTACAGTTGTTCGAAACATTATAATTCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCt                                                      <  1:357275/250‑1 (MQ=255)                                                                                                                                                                                                ccAATAAAGATCTGTATAAAAAGACAGTAATTCTACCTAGGGCAAAGCATTGCCCCGGGTATTACAGTTGTTCGAAACATTATAATTCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAgg                                                                                                                       <  1:285552/181‑1 (MQ=255)                                                                                                                                                                                                 cAATAAAGATCTGTATAAAAAGACAGTAATTCTACCTAGGGCAAAGCATTGCCCCGGGTATTACAGTTGTTCGAAACATTATAATTCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGt                                                >  1:807303/1‑251 (MQ=255)                                                                                                                                                                                                   aTAAAGATCTGTATAAAAAGACAGTAATTCTACCTAGGGCAAAGCATTGCCCCGGGTATTACAGTTGTTCGAAACATTATAATTCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGc                                                                                                                                           >  1:607509/1‑158 (MQ=25)                                                                                                                                                                                                          tCTGTATAAAAAGACAGTAATTCTACCTAGGGCAAAGCATTGCCCCGGGTATTACAGTTGTTCGAAACATTATAATTCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCAATGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAg                                       <  1:686507/251‑1 (MQ=255)                                                                                                                                                                                                             gTATAAAAAGACAGTAATTCTACCTAGGGCAAAGCATTGCCCCGGGTATTAc                                                                                                                                                                                                                                            >  1:1677/1‑52 (MQ=255)                                                                                                                                                                                                             gTATAAAAAGACAGTAATTCTACCTAGGGCAAAGCATTGCCCCGGGTATTACAGTTGTTCGAAACATTATAATTCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGct                                                                                                                                          <  1:590411/149‑1 (MQ=21)                                                                                                                                                                                                              tataAAAAGACAGTAATTCTACCTAGGGCAAAGCATTGCCCCGGGTATTACAGTTGTTCGAAACATTATAATTCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCt                                                      <  1:520546/232‑1 (MQ=255)                                                                                                                                                                                                                        cAGTAATTCTACCTAGGGCAAAGCATTGCCCCGGGTATTACAGTTGTTCGAAACATTATAAt                                                                                                                                                                                                                       >  1:206894/1‑62 (MQ=255)                                                                                                                                                                                                                           tAATTCTACCTAGGGCAAAGCATTGCCCCGGGTATTACAGTTGTTCGAAACATTATAATTCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAAttt                      >  1:621061/1‑250 (MQ=255)                                                                                                                                                                                                                             aTTCTACCTAGGGCAAAGCATTGCCCCGGGTATTACAGTTGTTCGAAACATTATAATTCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATg                                                 <  1:551652/222‑1 (MQ=255)                                                                                                                                                                                                                                  aCCTAGGGCAAAGCATTGCCCCGGGTATTACAGTTGTTCGAAACATtat                                                                                                                                                                                                                          <  1:538236/49‑1 (MQ=255)                                                                                                                                                                                                                                  aCCTAGGGCAAAGCATTGCCCCGGGTATTACAGTTGTTCGAAACATTATAATTCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAgg                                                                                                                       >  1:629812/1‑147 (MQ=18)                                                                                                                                                                                                                                  aCCTAGGGCAAAGCATTGCCCCGGGTATTACAGTTGTTCGAAACATTATAATTCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGAtttt               >  1:679621/1‑250 (MQ=255)                                                                                                                                                                                                                                       gggCAAAGCATTGCCCCGGGTATTACAGTTGTTCGAAACATTATAATTCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGc                                                                                                                                                               <  1:616685/102‑1 (MQ=18)                                                                                                                                                                                                                                       gggCAAAGCATTGCCCCGGGTATTACAGTTGTTCGAAACATTATAATTCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGc                                                                                                                                           <  1:16384/122‑1 (MQ=18)                                                                                                                                                                                                                                       gggCAAAGCATTGCCCCGGGTATTACAGTTGTTCGAAACATTATAATTCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAAt                                                                                        <  1:540947/173‑1 (MQ=255)                                                                                                                                                                                                                                       gggCAAAGCATTGCCCCGGGTATTACAGTTGTTCGAAACATTATAATTCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGAttttgccca          >  1:824935/1‑245 (MQ=14)                                                                                                                                                                                                                                              gCATTGCCCCGGGTATTACAGTTGTTCGAAACATTATAATTCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAggg   >  1:705025/1‑251 (MQ=14)                                                                                                                                                                                                                                              gCATTGCCCCGGGTATTACAGTTGTTCGAAACATTATAATTCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAggg   >  1:845409/1‑251 (MQ=14)                                                                                                                                                                                                                                              gCATTGCCCCGGGTATTACAGTTGTTCGAAACATTATAATTCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAggg   >  1:50152/1‑251 (MQ=14)                                                                                                                                                                                                                                               cATTGCCCCGGGTATTACAGTTGTTCGAAACATTATAATTCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAGGGc  <  1:368847/251‑1 (MQ=14)                                                                                                                                                                                                                                               cATTGCCCCGGGTATTACAGTTGTTCGAAACATTATAATTCATTGATA‑TTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCATTGGCTCTGCCAACGCCCCCAAAGGAGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTTAGATTTTGCCCACTCAggg   <  1:126165/250‑1 (MQ=14)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                     CACGGTTAGACCGTCAAACTGCGCGGCAATTTCGCTAATGGCGAGGCTATCCGCACCGGGCAGGATATTGGCTAAAATTTTATGATCATTCGCTTGGGTAGGAATTTCGAGATGAAAAAATTGAGTTTGCATAAAACGACAAAAGAGTAAGAAAAAAATTGCCGTTAGTTTAGCGCAAACGAGGGGGATTTCCAATAAAGATCTGTATAAAAAGACAGTAATTCTACCTAGGGCAAGGCATTGCCTCGGGTATTACAGTTGTTCGAAACATTATAATTCATTGATATTTTTTTAAATATCTTGCCTTCCTCCGTTTACGGGGGGACAAAACGCATTGCGTTTTGAGCGTTGGCTCTGCCAACGCCCCCAAAGGGGTGAGCAAGTAAGTTTACGAACTTGCGAATAACGTGCCCGAAGGGCGGAAGGGGGGATTTATCTTGATGTTTTCACAAGATCATGCTTAAAAATTGAGATTTCCCCCCCTCAGGGC  >  1/1473355‑1473844

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑