| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | 1 | 1,473,034 | T→C | E151E (GAA→GAG) | unknown ← | Transcription‑repair coupling factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | 1 | 1,473,034 | 0 | T | C | 98.7% | 212.1 / ‑5.1 | 76 | E151E (GAA→GAG) | unknown | Transcription‑repair coupling factor |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (35/40); total (36/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.74e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01 | |||||||||||
GAAAAATTCAATACCCGTGCTATCCGTCGGAAATTCATGTGCCGGCAATAAATTAATTTGGTCGATTTCCTGCAACGTACGCTGGGTGTCCACATCAAAAGTGCGAATGGAATCAATTTCATCATCAAAAAAATCTAAGCGAAAAGGCACCGCACTTCCCATC‑GGGAACAAATCCAACAACGCACCGCGCACCGCATATTCGCCATGTTCCAATACCTGCTCCACCGCACGATAACCCGCTTTTTCCAATTGTAGGCGAAGAGATTCGATGACAAAACGATCGCCTTTATTAATCAACAATACGTTATGCTGCAAATAAGACGGCGGGCAAACCCGTTGCATTAAGGTGGCGACAGGTAAAATGAAAATGCCTTGTTTGCGCTGTTGCAAATGAAACAAAGCGCTCAAGCGCGCGGAAATAATATCTTGATGAGGCGAAAAACTATCATAAGGCAGGGTTTCC > 1/1472791‑1473253 | gAAAAATTCAATACCCGTGCTATCCGTCGGAAATTCATGTGCCGGCAATAAATTGATTTGGTCGATTTCCTGCAACGTACGCTGGGTGTCCACATCAAAAGTGCGAATGGAATCAATTTCATCATCAAAAAAATCTAAGCGAAAAGGCACCGCACTTCCCATC‑GGGAACAAATCCAACAACGCACCGCGCACCGCATATTCGCCATGTTCCAATACCTGCTCCACCGCACGATAACCCGCTTTCTCCAAtt > 1:621709/1‑251 (MQ=255) aaaaaTTCAATACCCGTGCTATCCGTCGGAAATTCATGTGCCGGCAATAAATTGATTTGGTCGATTTCCTGCAACGTACGCTGGGTGTCCACATCAAAAGTGCGAATGGAATCAATTTCATCATCAAAAAAATCTAAGCGAAAAGGCACCGCACTTCCCATC‑GGGAACAAATCCAACAACGCGCCGCGCGCCGCATATTCGCCATGTTCCAATACCTGCTCCACCGCACGATAACCCGCTTTCTCCAATTg < 1:635903/251‑1 (MQ=255) aaaaaTTCAATACCCGTGCTATCCGTCGGAAATTCATGTGCCGGCAATAAATTGATTTGGTCGATTTCCTGCAACGTACGCTGGGTGTCCACATCAAAAGTGCGAATGGAATCAATTTCATCATCAAAAAAATCTAAGCGAAAAGGCACCGCACTTCCCATC‑GGGAACAAATCCAACAACGCACCGCGCACCGCATATTCGCCATGTTCCAATACCTGCTCCACCGCACGATAACCCGCTTTCTCCAATTg < 1:383390/251‑1 (MQ=255) aTTCAATACCCGTGCTATCCGTCGGAAATTCATGTGCCGGCAATAAATTGATTTGGTCGATTTCCTGCAACGTACGCTGGGTGTCCACATCAAAAGTGCGAATGGAATCAATTTCATCATCAAAAAAATCTAAGCGAAAAGGCACCGCACTTCCCATC‑GGGAACAAATCCAACAACGCACCGCGCACCGCATATTCGCCATGTTCCAATACCTGCTCCACCGCACGATAACCCGCTTTCTCCAATTGCAgg > 1:10788/1‑251 (MQ=255) tACCCGTGCTATCCGTCGGAAATTCATGTGCCGGCAATAAATTGATTTGGTCGATTTCCTGCAACGTACGCTGGGTGTCCACATCAAAAGTGCGAATGGAATCAATTTCATCATCAAAAAAATCTAAGCGAAAAGGCACCGCACTTCCCATC‑GGGAACAAATCCAACAACGCACCGCGCACCGCATATTCGCCATGTTCCAATACCTGCTCCACCGCACGATAACCCGCTTTCTCCAATTGCAGGCGAaga > 1:199070/1‑251 (MQ=255) aCCCGTGCTATCCGTCGGAAATTCATGTGCCGGCAATAAATTGATTTGGTCGATTTCCTGCAACGTACGCTGGGTGTCCACATCAAAAGTGCGAATGGAATCAATTTCATCATCAAAAAAATCTAAGCGAAAAGGCACCGCACTTCCCATC‑GGGAACAAATCCAACAACGCACCGCGCACCGCATATTCGCCATGTTCCAATACCTGCTCCACCGCACGATAACCCGCTTTCTCCAATTGCAGGCGAagag < 1:353081/251‑1 (MQ=255) gTGCTATCCGTCGGAAATTCATGTGCCGGCAATAAATTGATTTGGTCGATTTCCTGCAACGTACGCTGGGTGTCCACATCAAAAGTGCGAATGGAATCAATTTCATCATCAAAAAAATCTAAGCGAAAAGGCACCGCACTTCCCATC‑GGGAACAAATCCAACAACGCACCGCGCACCGCATATTCGCCATGTTCCAATACCTGCTCCACCGCACGATAACCCGCTTTCTCCAATTGCAGGCGAAGAGAtt > 1:510705/1‑250 (MQ=255) gTGCTATCCGTCGGAAATTCATGTGCCGGCAATAAATTAATTTGGTCGATTTCCTGCAACGTACGCTGGGTGTCCACATCAAAAGTGCGAATGGAATCAATTTCATCATCAAAAAAATCTAAGCGAAAAGGCACCGCACTTCCCATC‑GGGAACAAATCCAACAACGCACCGCGCACCGCATATTCGCCATGTTCCAATACCTGCTCCACCGCACGATAACCCGCTTTTTCCAATTGTAGGCGAAGAGAtt > 1:99935/1‑250 (MQ=255) aTCCGTCGGAAATTCATGTGCCGGCAATAAATTGATTTGGTCGATTTCCTGCAACGTACGCTGGGTGTCCACATCAAAAGTGCGAATGGAATCAATTTCATCATCAAAAAAATCTAAGCGAAAAGGCACCGCACTTCCCATC‑GGGAACAAATCCAACAACGCACCGCGCACCGCATATTCGCCATGTTCCAATACCTGCTCCACCGCACGATAACCCGCTTTCTCCAATTGCAGGCGAAGAGATTc > 1:56571/1‑246 (MQ=255) ccGGCAATAAATTGATTTGGTCGATTTCCTGCAACGTACGCTGGGTGTCCACATCAAAAGTGCGAATGGAATCAATTTCATCATCAAAAAAATCTAAGCGAAAAGGCACCGCACTTCCCATC‑GGGAACAAATCCAACAACGCACCGCGCACCGCATATTCGCCATGTTCCAATACCTGCTCCACCGCACGATAACCCGCTTTCTCCAATTGCAGGCGAAGAGATTCGATGACAAAACGATCGCCTttatta < 1:782436/251‑1 (MQ=255) tAAATTGATTTGGTCGATTTCCTGCAACGTACGCTGGGTGTCCACATCAAAAGTGCGAATGGAATCAATTTCATCATCAAAAAAATCTAAGCGAAAAGGCACCGCACTTCCCATC‑GGGAACAAATCCAACAACGCACCGCGCACCGCATATTCGCCATGTTCCAATACCTGCTCCACCGCACGATAACCCGCTTTCTCCAATTGCAGGCGAAGAGATTCGATGACAAAACGATCGCCTTTATTAATcaaca > 1:514247/1‑251 (MQ=255) ttgattTGGTCGATTTCCTGCAACGTACGCTGGGTGTCCACATCAAAAGTGCGAATGGAATCAATTTCATCATCAAAAAAATCTAAGCGAAAAGGCACCGCACTTCCCATC‑GGGAACAAATCCAACAACGCACCGCGCACCGCATATTCGCCATGTTCCAATACCTGCTCCACCGCACGATAACCCGCTTTCTCCAATTGCAGGCGAAGAGATTCGATGACAAAACGATCGCCTTTATTAATCAACAATAc < 1:775325/248‑1 (MQ=255) attTGGTCGATTTCCTGCAACGTACGCTGGGTGTCCACATCAAAAGTGCGAATGGAATCAATTTCATCATCAAAAAAATCTAAGCGAAAAGGCACCGCACTTCCCATC‑GGGAACAAATCCAACAACGCACCGCGCACCGCATATTCGCCATGTTCCAATACCTGCTCCACCGCACGATAACCCGCTTTCTCCAATTGCAGGCGAAGAGATTCGATGACAAAACGATCGCCTTTATTAATCAACAATACGt < 1:223066/250‑1 (MQ=255) attTGGTCGATTTCCTGCAACGTACGCTGGGTGTCCACATCAAAAGTGCGAATGGAATCAATTTCATCATCAAAAAAATCTAAGCGAAAAGGCACCGCACTTCCCATC‑GGGAACAAATCCAACAACGCACCGCGCACCGCATATTCGCCATGTTCCAATACCTGCTCCACCGCACGATAACCCGCTTTCTCCAATTGCAGGCGAAGAGATTCGATGACAAAACGATCGCCTTTATTAATCAACAATACGt < 1:690585/250‑1 (MQ=255) tttGGTCGATTTCCTGCAACGTACGCTGGGTGTCCACAACAAAAGTGCGAATGGAATCAATTTCATCATCAAAAAAATCTAAGCGAAAAGGCACCGCACTTCCCATCGGGGAACAAATCCAACAACGCACCGCGCACCGCATATTCGCCATGTTCCAATACCTGCTCCACCGCACGATAACCCGCTTTCTCCAATTGCAGGCGAAGAGATTCGATGACAAAACGATCGCCTTTATTAATCAACAATACGtt < 1:520072/251‑1 (MQ=255) aTTTCCTGCAACGTACGCTGGGTGTCCACATCAAAAGTGCGAATGGAATCAATTTCATCATCAAAAAAATCTAAGCGAAAAGGCACCGCACTTCCCATC‑GGGAACAAATCCAACAACGCACCGCGCACCGCATATTCGCCATGTTCCAATACCTGCTCCACCGCACGATAACCCGCTTTCTCCAATTGCAGGCGAAGAGATTCGATGACAAAACGATCGCCTTTATTAATCAACAATACGTTATGCTGCaa > 1:302861/1‑251 (MQ=255) ttCCTGCAACGTACGCTGGGTGTCCACATCAAAAGTGCGAATGGAATCAATTTCATCATCAAAAAAATCTAAGCGAAAAGGCACCGCACTTCCCATC‑GGGAACAAATCCAACAACGCACCGCGCACCGCATATTCGCCATGTTCCAATACCTGCTCCACCGCACGATAACCCGCTTTCTCCAATTGCAGGCGAAGAGATTCGATGACAAAACGATCGCCTTTATTAATCAACAATACGTTATGCTGCAAAt > 1:485102/1‑251 (MQ=255) ccTGCAACGTACGCTGGGTGTCCACATCAAAAGTGCGAATGGAATCAATTTCATCATCAAAAAAATCTAAGCGAAAAGGCACCGCACTTCCCATC‑GGGAACAAATCCAACAACGCACCGCGCACCGCATATTCGCCATGTTCCAATACCTGCTCCACCGCACGATAACCCGCTTTCTCCAATTGCAGGCGAAGAGATTCGATGACAAAACGATCGCCTTTATTAATCAACAATACGTTATGCTGCAAATaa > 1:544779/1‑251 (MQ=255) cAACGTACGCTGGGTGTCCACATCAAAAGTGCGAATGGAATCAATTTCATCATCAAAAAAATCTAAGCGAAAAGGCACCGCACTTCCCATC‑GGGAACAAATCCAACAACGCACCGCGCACCGCATATTCGCCATGTTCCAATACCTGCTCCACCGCACGATAACCCGCTTTCTCCAATTGCAGGCGAAGAGATTCGATGACAAAACGATCGCCTTTATTAATCAACAATACGTTATGCTGCAAATAAGAc < 1:890931/250‑1 (MQ=255) aaCGTACGCTGGGTGTCCACATCAAAAGTGCGAATGGAATCAATTTCATCATCAAAAAAATCTAAGCGAAAAGGCACCGCACTTCCCATC‑GGGAACAAATCCAACAACGCACCGCGCACCGCATATTCGCCATGTTCCAATACCTGCTCCACCGCACGATAACCCGCTTTCTCCAATTGCAGGCGAAGAGATTCGATGACAAAACGATCGCCTTTATTAATCAACAATACGt > 1:430448/1‑232 (MQ=255) aaCGTACGCTGGGTGTCCACATCAAAAGTGCGAATGGAATCAATTTCATCATCAAAAAAATCTAAGCGAAAAGGCACCGCACTTCCCATC‑GGGAACAAATCCAACAACGCACCGCGCACCGCATATTCGCCATGTTCCAATACCTGCTCCACCGCACGATAACCCGCTTTCTCCAATTGCAGGCGAAGAGATTCGATGACAAAACGATCGCCTTTATTAATCAACAATACGt < 1:627333/232‑1 (MQ=255) aaCGTACGCTGGGTGTCCACATCAAAAGTGCGAATGGAATCAATTTCATCATCAAAAAAATCTAAGCGAAAAGGCACCGCACTTCCCATC‑GGGAACAAATCCAACAACGCACCGCGCACCGCATATTCGCCATGTTCCAATACCTGCTCCACCGCACGATAACCCGCTTTCTCCAATTGCAGGCGAAGAGATTCGATGACAAAACGATCGCCTTTATTAATCAACAATACGTTATGCTGCAAATAAGAcgg > 1:60036/1‑251 (MQ=255) aCGTACGCTGGGTGTCCACATCAAAAGTGCGAATGGAATCAATTTCATCATCAAAAAAATCTAAGCGAAAAGGCACCGCACTTCCCATC‑GGGAACAAATCCAACAACGCACCGCGCACCGCATATTCGCCATGTTCCAATACCTGCTCCACCGCACGATAACCCGCTTTCTCCAATTGCAGGCGAAGAGATTCGATGACAAAACGATCGCCTTTATTAATCAACAATACGTTATGCTGCAAATAAGAcgg > 1:417880/1‑250 (MQ=255) gTACGCTGGGTGTCCACATCAAAAGTGCGAATGGAATCAATTTCATCATCAAAAAAATCTAAGCGAAAAGGCACCGCACTTCCCATC‑GGGAACAAATCCAACAACGCACCGCGCACCGCATATTCGCCATGTTCCAATACCTGCTCCACCGCACGATAACCCGCTTTCTCCAATTGCAGGCGAAGAGATTCGATGACAAAACGATCGCCTttatta > 1:119162/1‑216 (MQ=255) cGCTGGGTGTCCACATCAAAAGTGCGAATGGAATCAATTTCATCATCAAAAAAATCTAAGCGAAAAGGCACCGCACTTCCCATC‑GGGAACAAATCCAACAACGCACCGCGCACCGCATATTCGCCATGTTCCAATACCTGCTCCACCGCACGATAACCCGCTTTCTCCAATTGCAGGCGAAGAGATTCGATGACAAAACGATCGCCTTTATTAATCAACAATACGTTATGCTGCAAATaa < 1:198270/240‑1 (MQ=255) cGCTGGGTGTCCACATCAAAAGTGCGAATGGAATCAATTTCATCATCAAAAAAATCTAAGCGAAAAGGCACCGCACTTCCCATC‑GGGAACAAATCCAACAACGCACCGCGCACCGCATATTCGCCATGTTCCAATACCTGCTCCACCGCACGATAACCCGCTTTCTCCAATTGCAGGCGAAGAGATTCGATGACAAAACGATCGCCTTTATTAATCAACAATACGTTATGCTGCAAATAAGACGGCGGGCa > 1:780807/1‑251 (MQ=255) gggTGTCCACATCAAAAGTGCGAATGGAATCAATTTCATCATCAAAAAAATCTAAGCGAAAAGGCACCGCACTTCCCATC‑GGGAACAAATCCAACAACGCACCGCGCACCGCATATTCGCCATGTTCCAATACCTGCTCCACCGCACGATAACCCGCTTTCTCCAATTGCAGGCGAAGAGATTCGATGACAAAACGATCGCCTTTATTAATCAACAATACGTTATGCTGCAAATAAGACGGCGGGCAAAc > 1:876656/1‑250 (MQ=255) gtCCACATCAAAAGTGCGAATGGAATCAATTTCATCATCAAAAAAATCTAAGCGAAAAGGCACCGCACTTCCCATC‑GGGAACAAATCCAACAACGCACCGCGCACCGCATATTCGCCATGTTCCAATACCTGCTCCACCGCACGATAACCCGCTTTCTCCAATTGCAGGCGAAGAGATTCGATGACAAAACGATCGCCTTTATTAATCAACAATACGt < 1:502523/218‑1 (MQ=255) tCCACATCAAAAGTGCGAATGGAATCAATTTCATCATCAAAAAAATCTAAGCGAAAAGGCACCGCACTTCCCATC‑GGGAACAAATCCAACAACGCACCGCGCACCGCATATTCGCCATGTTCCAATACCTGCTCCACCGCACGATAACCCGCTTTCTCCAATTGCAGGCGAAGAGATTCGATGACAAAACGATCGCCTTTATTAATCAACAATACGTTATGCTGCAAATAAGACGGCGGGCAAACCCGTTg < 1:299986/251‑1 (MQ=255) acaTCAAAAGTGCGAATGGAATCAATTTCATCATCAAAAAAATCTAAGCGAAAAGGCACCGCACTTCCCATC‑GGGAACAAATCCAACAACGCACCGCGCACCGCATATTCGCCATGTTCCAATACCTGCTCCACCGCACGATAACCCGCTTTCTCCAATTGCAGGCGAAGAGATTCGATGACAAAACGATCGCCTTTATTAATCAACAATACGTTATGCTGCAAATAAGACGGCGGGCAAAcc < 1:155599/243‑1 (MQ=255) caTCAAAAGTGCGAATGGAATCAATTTCATCATCAAAAAAATCTAAGCGAAAAGGCACCGCACTTCCCATC‑GGGAACAAATCCAACAACGCACCGCGCACCGCATATTCGCCATGTTCCAATACCTGCTCCACCGCACGATAACCCGCTTTCTCCAATTGCAGGCGAAGAGATTCGATGACAAAACGATCGCCTTTATTAATCAACAATACGTTATGCTGCaa < 1:452504/223‑1 (MQ=255) caTCAAAAGTGCGAATGGAATCAATTTCATCATCAAAAAAATCTAAGCGAAAAGGCACCGCACTTCCCATC‑GGGAACAAATCCAACAACGCACCGCGCACCGCATATTCGCCATGTTCCAATACCTGCTCCACCGCACGATAACCCGCTTTCTCCAATTGCAGGCGAAGAGATTCGATGACAAAACGATCGCCTTTATTAATCAACAATACGTTATGCTGCAAATAAGAc < 1:399941/230‑1 (MQ=255) caTCAAAAGTGCGAATGGAATCAATTTCATCATCAAAAAAATCTAAGCGAAAAGGCACCGCACTTCCCATC‑GGGAACAAATCCAACAACGCACCGCGCACCGCATATTCGCCATGTTCCAATACCTGCTCCACCGCACGATAACCCGCTTTCTCCAATTGCAGGCGAAGAGATTCGATGACAAAACGATCGCCTTTATTAATCAACAATACGTTATGCTGCAAATAAGACGGCGGGCAAACCCGTTGCAtt > 1:743653/1‑251 (MQ=255) aaaaGTGCGAATGGAATCAATTTCATCATCAAAAAAATCTAAGCGAAAAGGCACCGCACTTCCCATC‑GGGAACAAATCCAACAACGCACCGCGCACCGCATATTCGCCATGTTCCAATACCTGCTCCACCGCACGATAACCCGCTTTCTCCAATTGCAGGCGAAGAGATTCGATGACAAAACGATCGCCTTTATTAATCAACAATACGTTATGCTGCAAATAAGACGGCGGGCAAACCCGTTGCATTaaag < 1:144622/251‑3 (MQ=255) aaTGGAATCAATTTCATCATCAAAAAAATCTAAGCGAAAAGGCACCGCACTTCCCATC‑GGGAACAAATCCAACAACGCACCGCGCACCGCATATTCGCCATGTTCCAATACCTGCTCCACCGCACGATAACCCGCTTTCTCCAATTGCAGGCGAAGAGATTCGATGACAAAACGATCGCCTTTATTAATCAACAATACGt > 1:55231/1‑200 (MQ=255) gAATCAATTTCATCATCAAAAAAATCTAAGCGAAATGGCACCGCACTTCCCATC‑GGGAACAAATCCAACAACGCACCGCGCACCGCATATTCGCCATGTTCCAATACCTGCTCCACCGCACGATAACCCGCTTTCTCCAATTGCAGGCGAAGAGATTCGATGACAAAACg > 1:893172/1‑170 (MQ=255) tcatcatcaAAAAAATCTAAGCGAAAAGGCACCGCACTTCCCATC‑GGGAACAAATCCAACAACGCACCGCGCACCGCATATTCGCCATGTTCCAATACCTGCTCCACCGCACGATAACCCGCTTTCTCCAATTGCAGGCGAAGAGATTCGATGACAAAACGATCGCCTTTATTAATCAACAATACGTTATGCTGCAAATAAGACGGCGGGCAAACCCGTTGCATTAAAGTGGCGACAGGTaaaatgaaaat < 1:93556/251‑1 (MQ=255) tcaAAAAAATCTAAGCGAAAAGGCACCGCACTTCCCATC‑GGGAACAAATCCAACAACGCACCGCGCACCGCATATTCGCCATGTTCCAATACCTGCTCCACCGCACGATAACCCGCTTTCTCCAATTGCAGGCGAAGAGATTCGATGACAAAACGATCGCCTTTATTAATCAACAATACGTTATGCTGCAAATAAGACGGCGGGCAAACCCGTTGCATTAAAGTGGCGACAGGTAAAATGAAAATGCCTTg > 1:125823/1‑251 (MQ=255) aaaaaaaTCTAAGCGAAAAGGCACCGCACTTCCCATC‑GGGAACAAATCCAACAACGCACCGCGCACCGCATATTCGCCATGTTCCAATACCTGCTCCACCGCACGATAACCCGCTTTCTCCAATTGCAGGCGAAGAGATTCGATGACAAAACGATCGCCTTTATTAATCAACAATACGTTATGCTGCAAATAAGACGGCGGGCAAACCCGTTGCATTAAAGTGGCGACAGGTAAAATGAAAATGCCTTGtt < 1:559771/251‑1 (MQ=255) gCGAAAAGGCACCGCACTTCCCATC‑GGGAACAAATCCAACAACGCACCGCGCACCGCATATTCGCCATGTTCCAATACCTGCTCCACCGCACGATAACCCGCTTTCTCCAATTGCAGGCGAAGAGATTCGATGACAAAACGATCGCCTTTATTAATCAACAATACGTTATGCTGCAAATAAGAc < 1:259301/184‑1 (MQ=255) gCGAAAAGGCACCGCACTTCCCATC‑GGGAACAAATCCAACAACGCACCGCGCACCGCATATTCGCCATGTTCCAATACCTGCTCCACCGCACGATAACCCGCTTTCTCCAATTGCAGGCGAAGAGATTCGATGACAAAACGATCGCCTTTATTAATCAACAATACGTTATGCTGCAAATAAGACGGCGGGCAAACCCGTTGCATTAAAGTGGCGACAGGTAAAATGAAAATGCCTTGTTTGCGCTGTTGCa > 1:765932/1‑251 (MQ=255) gCGAAAAGGCACCGCACTTCCCATC‑GGGAACAAATCCAACAACGCACCGCGCACCGCATATTCGCCATGTTCCAATACCTGCTCCACCGCACGATAACCCGCTTCCTCCAATTGCAGGCGAAGAGATTCGATGACAAAACGATCGCCTTTATTAATCAACAATACGTTATGCTGCAAATAAGACGGCGGGCAAACCCGTTGCATTAAAGTGGCGACAGGTAAAATGAAAATGCCTTGTTTGCGCTGTTGCa > 1:204782/1‑251 (MQ=255) cGAAAAGGCACCGCACTTCCCATC‑GGGAACAAATCCAACAACGCACCGCGCACCGCATATTCGCCATGTTCCAATACCTGCTCCACCGCACGATAACCCGCTTTCTCCAATTGCAGGCGAAGAGATTCGATGACAAAACGATCGCCTTTATTAATCAACAATACGTTATGCTGCAAATAAGACGGCGGGCAAACCCGTTGCATTAAAGTGGCGACAGGTAAAATGAAAATGCCTTGTTTGCGCTGTTGCaa < 1:285857/251‑1 (MQ=255) ggCACCGCACTTCCCATC‑GGGAACAAATCCAACAACGCACCGCGCACCGCATATTCGCCATGTTCCAATACCTGCTCCACCGCACGATAACCCGCTTTCTCCAATTGCAGGCGAAGAGATTCGATGACAAAACGATCGCCTTTATTAATCAACAATACGTTATGCTGCAAATAAGACGGCGGGCAAACCCGTTGCATTaa < 1:215621/200‑1 (MQ=255) gCACCGCACTTCCCATC‑GGGAACAAATCCAACAACGCACCGCGCACCGCATATTCGCCATGTTCCAATACCTGCTCCACCGCACGATAACCCGCTTTCTCCAATTGCAGGCGAAGAGATTCGATGACAAAACGAt > 1:592686/1‑135 (MQ=255) ccGCACTTCCCATC‑GGGAACAAATCCAACAACGCACCGCGCACCGCATATTCGCCATGTTCCAATACCTGCTCCACCGCACGATAACCCGCTTTCTCCAATTGCAGGCGAAGAGATTCGATGACAAAACGATCGCCTTTATTAATCAACAATACGTTATGCTGCAAATAAGACGGCGGGCAAACCCGTTGCATTAAAGTGGCGACAGGTAAAATGAAAATGCCTTGTTTGCGCTGTTGCAAATGAAACaaa > 1:577446/1‑251 (MQ=255) tCCCATC‑GGGAACAAATCCAACAACGCACCGCGCACCGCATATTCGCCATGTTCCAATACCTGCTCCACCGCACGATAACCCGCTTTCTCCAATTGCAGGCGAAGAGATTCGATGACAAAACGATCGCCTTTATTAATCAACAATACGTTATGCTGCAAATAAGACGGCGGGCAAACCCGTTGCATTAAAGTGGCGACAGGTAAAATGGAAATGCCTTGTTTGCGCTGTTGCAAATGAAACAAAGCGCTCa > 1:217955/1‑251 (MQ=255) ccATC‑GGGAACAAATCCAACAACGCACCGCGCACCGCATATTCGCCATGTTCCAATACCTGCTCCACCGCACGATAACCCGCTTTCTCCAATTGCAGGCGAAGAGATTCGATGACAAAACGATCGCCTTTATTAATCAACAATACGTTATGCTGCAAATAAGACGGCGGGCAAACCCGTTGCATTAAAGTGGCGACAGGTAAAATGAAAATGCCTTGTTTGCGCTGTTGCAAATGAAACAAAGCGCTCAAg < 1:732360/251‑1 (MQ=255) aTC‑GGGAACAAATCCAACAACGCACCGCGCACCGCATATTCGCCATGTTCCAATACCTGCTCCACCGCACGATAACCCGCTTTCTCCAATTGCAGGCGAAGAGATTCGATGACAAAACGATCGCCTTTATTAATCAACAATACGTTATGCTGCAAATAAGACGGCGGGCAAACCCGTTGCATTAAAGTGGCGACAGGTAAAATGAAAATGCCTTGTTTGCGCTGTTGCAAATGAAACAAAGCGCTCAAgcg < 1:865375/251‑1 (MQ=255) aTC‑GGGAACAAATCCAACAACGCACCGCGCACCGCATATTCGCCATGTTCCAATACCTGCTCCACCGCACGATAACCCGCTTTCTCCAATTGCAGGCGAAGAGATTCGATGACAAAACGATCGCCTTTATTAATCAACAATACGTTATGCTGCAAATAAGACGGCGGGCAAACCCGTTGCATTAAAGTGGCGACAGGTAAAATGAAAATGCCTTGTTTGCGCTGTTGCAAATGAAACAAAGCGCTCAAgcg < 1:824628/251‑1 (MQ=255) aaCAAATCCAACAACGCACCGCGCACCGCATATTCGCCATGTTCCAATACCTGCTCCACCGCACGATAACCCGCTTTCTCCAATTGCAGGCGAAGAGATTCGATGACAAAACGATCGCCTTTATTAATCAACAATACGTTATGCTGCAAATAAGACGGCGGGCAAACCCGTTGCATTAAAGTGGCGACAGGTAAAATGAAAATGCCTTGTTTGCGCTGTTGCAAATGAAACAAAGCGCTCAAGCGCGCgg < 1:25659/250‑1 (MQ=255) ccAACAACGCACCGCGCACCGCATATTCGCCATGTTCCAATACCTGCTCCACCGCACGATAACCCGCTTTCTCCAATTGCAGGCGAAGAGATTCGATGACAAAACGATCGCCTTTATTAATCAACAATACGTTATGCTGCAAATAAGACGGCGGGCAAACCCGTTGCATTAAGGTGGCGACAGGTAAAATGAAAATGCCTTGTTTGCGCTGTTGCAAATGAAACAAAGCGCTCAAGCGCGCGGAAataata < 1:869456/251‑1 (MQ=255) acaacGCACCGCGCACCGCATATTCGCCATGTTCCAATACCTGCTCCACCGCACGATAACCCGCTTTCTCCAATTg < 1:270148/76‑1 (MQ=255) acaacGCACCGCGCACCGCATATTCGCCATGTTCCAATACCTGCTCCACCGCACGATAACCCGCTTTCTCCAATTGCAGGCGAAGAGATTCGATGACAAAACGATCGCCTTTATTAATcaacaa < 1:608749/124‑1 (MQ=255) caccgcgcaccgcATATTCGCCATGTTCCAATACCTGCTCCACCGCACGATAACCCGCTTTCTCCAATTGCAGGCGAAGAGATTCGATGACAAAACGATCGCCTTTATTAATCAACAATACGTTATGCTGCAAATAAGACGGCGGGCAAACCCGTTGCATTAAAGTGGCGACAGGTAAAATGAAAATGCCTTGTTTGCGCTGTTGCAAATGAAACAAAGCGCTCAAGCGCGCGGAAATAatat > 1:218237/1‑243 (MQ=255) caccgcATATTCGCCATGTTCCAATACCTGCTCCACCGCACGATAACCCGCTTTCTCCAATTGCAGGCGAAGAGATTCGATGACAAAACGATCGCCTTTATTAATCAACAATACGTTATGCTGCAAATAAGACGGCGGGCAAACCCGTTGCATTAAAGTGGCGACAGGTAAAATGAAAATGCCTTGTTTGCGCTGTTGCAAATGAAACAAAGCGCTCAAGCGCGCGGAAATAATATCTtgatg > 1:827429/1‑243 (MQ=255) accgcATATTCGCCATGTTCCAATACCTGCTCCACCGCACGATAACCCGCTTTCTCCAATTGCAGGCGAAGAGATTCGATGACAAAACGATCGCCTTTATTAATCAACAATACGt > 1:149472/1‑115 (MQ=255) ccgcATATTCGCCATGTTCCAATACCTGCTCCACCGCACGATAACCCGCTTTCTCCAATTGCAGGCGAAGAGATTCGATGACAAAACGATCGCCTTTATTAATCAACAATACGTTATGCTGCAAATAAGACGGCGGGCAAACCCGTTGCATTaaag < 1:589601/156‑3 (MQ=255) cgcATATTCGCCATGTTCCAATACCTGCTCCACCGCACGATAACCCGCTTTCTCCAATTGCAGGCGAAGAGATTCGATGACAAAACGATCGCCTTTATTAATCAACAATACGTTATGCTGCAAATAAGACGGCGGGCAAACCCGTTGCATTAAAGTGGCGACAGGTAAAATGAAAATGCCTTGTTTGCGCTGTTGCAAATGAAACAAAGCGCTCAAGCGCGCGGAAATAATATCTTGATGAGGCGAAAAAc < 1:44288/251‑1 (MQ=255) ccATGTTCCAATACCTGCTCCACCGCACGATAACCCGCTTTCTCCAATTGCAGGCGAAGAGATTCGATGACAAAACGATCGCCTTTATTAATCAACAATACGTTAt < 1:897676/106‑1 (MQ=255) cATGTTCCAATACCTGCTCCACCGCACGATAACCCGCTTTCTCCAATTGCAGGCGAAGAGATTCGATGACAAAACGATCGCCTTTATTAATCAACAATACGTTATGCTGCAAATAAGACGGCGGGCAAACCCGTTGCATTAAAGTGGCGACAGGTAAAATGAAAATGCCTTGTTTGCGCTGTTGCAAATGAAACAAAGCGCTCAAGCGCGCGGAAATAATATCTTGATGAGGCGAAAAACTATCATAAGGc < 1:532448/251‑1 (MQ=255) gTTCCAATACCTGCTCCACCGCACGATAACCCGCTTTCTCCAATTGCAGGCGAAGAGATTCGATGACAAAACGATCGCCTTTATTAATCAACAATACGTTAtgctg > 1:865984/1‑106 (MQ=255) gTTCCAATACCTGCTCCACCGCACGATAACCCGCTTTCTCCAATTGCAGGCGAAGAGATTCGATGACAAAACGATCGCCTTTATTAATCAACAATACGTTAtgc < 1:856171/104‑1 (MQ=255) gTTCCAATACCTGCTCCACCGCACGATAACCCGCTTTCTCCAATTGCAGGCGAAGAGATTCGATGACAAAACGATCGCCTTTATTAATCAACAATACGTTATGCTGCAAATAAGACGGCGGGCAAACCCGTTGCATTAAAGTGGCGACAGGTAAAATGAAAATGCCTTGTTTGCGCTGTTGCAAATGAAACAAAGCGCTCAAGCGCGCGGAAataata < 1:680547/218‑1 (MQ=255) gTTCCAATACCTGCTCCACCGCACGATAACCCGCTTTCTCCAATTGCAGGCGAAGAGATTCGATGACAAAACGATCGCCTTTATTAATCAACAATACGTTATGCTGCAAATAAGACGGCGGGCAAACCCGTTGCATTAAAGTGGCGACAGGTAAAATGAAAATGCCTTGTTTGCGCTGTTGCAAATGAAACAAAGCGCTCAAGCGCGCGGAAATAATATCTTGATGAGGCGAAAAACTATCATAAGGCAgg > 1:871290/1‑251 (MQ=255) ttCCAATACCTGCTCCACCGCACGATAACCCGCTTTCTCCAATTGCAGGCGAAGAGATTCGATGACAAAACGATCGCCTTTATTAATCAACAAt < 1:360726/94‑1 (MQ=255) ttCCAATACCTGCTCCACCGCACGATAACCCGCTTTCTCCAATTGCAGGCGAAGAGATTCGATGACAAAACGATCGCCTTTATTAATCAACAATACGTTATGCTGCAAATAAGACGGC‑GGCAAACCCGTTGCATTAAAGTGGCGACAGGTAAAATGAAAATGCCTTGTTTGCGCTGTTGCAAATGAAACAAAGCGCTCAAGCGCGCGGAAATAATATCTTGATGAGGCGAAAAACTATCATAAGGCAGGGt < 1:452732/251‑1 (MQ=255) ttCCAACACCTGCTCCACCGCACGATAACCCGCTTTCTCCAATTGCAGGCGAAGAGATTCGATGACAAAACGATCGCCTTTATTAATCAACAATACGTTATGCTGCAAATAAGACGGCGGGCAAACCCGTTGCATTAAAGTGGCGACAGGTAAAATGAAAATGCCTTGTTTGCGCTGTTGCAAATGAAACAAAGCGCTCAAGCGCGCGGAAATAATATCTTGATGAGGCGAAAAACTATCATAAGGCAggg > 1:424178/1‑251 (MQ=255) tCCAATACCTGCTCCACCGCACGATAACCCGCTTTCTCCAATTGCAGGCGAAGAGATTCGATGACAAAAc > 1:831016/1‑70 (MQ=255) tCCAATACCTGCTCCACCGCACGATAACCCGCTTTCTCCAATTGCAGGCGAAGAGATTCGATGACAAAACGATCGCCTTTATTAATCAACAATACGTTATGCTGCAAATAAGACGGCGGGCAAACCCGTTGCATTAAAGTGGCGACAGGTAAAATGAAAATGCCTTGTTTGCGCTGTTGCAAATGAAACAAAGCGCTCAAGCGCGCGGAAATAATATCTTGATGAGGCGAAAAACTATCATAAGGCAGGGt > 1:234515/1‑251 (MQ=255) tCCAATACCTGCTCCACCGCACGATAACCCGCTTTCTCCAATTGCAGGCGAAGAGATTCGATGACAAAACGATCGCCTTTATTAATCAACAATACGTTATGCTGCAAATAAGACGGCGGGCAAACCCGTTGCATTAAAGTGGCGACAGGTAAAATGAAAATGCCTTGTTTGCGCTGTTGCAAATGAAACAAAGCGCTCAAGCGCGCGGAAATAATATCTTGATGAGGCGAAAAACTATCATAAGGCAGGGt < 1:34864/251‑1 (MQ=255) aTACCTGCTCCACCGCACGATAACCCGCTTTCTCCAATTGCAGGCGAAGAGATTCGATGACAAAACGATCGCCTTTATTAATCAACAATACGTTATGCTGCAAATAAGACGGCGGGCAAACCCGTTGCATTAAAGTGGCGACAGGTAAAATGAAAATGCCTTGTTTGCGCt < 1:322180/171‑1 (MQ=255) tACCTGCTCCACCGCACGATAACCCGCTTTCTCCAATTGCAGGCGAAGAGATTCGATGACAAAACGATCGCCTTTATTAATCAACAATACGTTATGCTGCAAATAAGACGGCGGGCAAACCCGTTGCATTAAAGTGGCGACAGGTAAAATGAAAATGCCTTGTTTGCGCTGTTGCAAATGAAACAAAGCGCTCAAGCGCGCGGAAATAATATCTTGATGAGGCGAAAAACTATCATAAGGCAGGGTTTcc > 1:665448/1‑250 (MQ=255) tGCTCCACCGCACGATAACCCGCTTTCTCCAATTGCAGGCGAAGAGATTCGATGACAAAACGATCGCCTTTATTAATCAACAATACGTTATGCTGCAAATAAGACGGCGGGCAAACCCGTTGCATTAAAGTGGCGACAGGTAAAATGAAAATGCCTTGTTTGCGCtaaactaact < 1:315661/175‑10 (MQ=255) aTAACCCGCTTTCTCCAATTGCAGGCGAAGAGATTCGATGACAAAACGATCGCCTttat > 1:675272/1‑59 (MQ=255) gCTTTCTCCAATTGCAGGCGAAGAGATTCGATGACAAAACGATCGCCTTTATTAATCAACAATACGTTATGCTGCAAATAAGACGGCGGGCAAACCCGTTGCATTAAAGTGGCGACAGGTAAAATGAAAATGCCTTGTTTGCGCTGTTGCAAATGAAACAAAGCGCTCAAGCGCGCGGAAataata > 1:550759/1‑186 (MQ=255) | GAAAAATTCAATACCCGTGCTATCCGTCGGAAATTCATGTGCCGGCAATAAATTAATTTGGTCGATTTCCTGCAACGTACGCTGGGTGTCCACATCAAAAGTGCGAATGGAATCAATTTCATCATCAAAAAAATCTAAGCGAAAAGGCACCGCACTTCCCATC‑GGGAACAAATCCAACAACGCACCGCGCACCGCATATTCGCCATGTTCCAATACCTGCTCCACCGCACGATAACCCGCTTTTTCCAATTGTAGGCGAAGAGATTCGATGACAAAACGATCGCCTTTATTAATCAACAATACGTTATGCTGCAAATAAGACGGCGGGCAAACCCGTTGCATTAAGGTGGCGACAGGTAAAATGAAAATGCCTTGTTTGCGCTGTTGCAAATGAAACAAAGCGCTCAAGCGCGCGGAAATAATATCTTGATGAGGCGAAAAACTATCATAAGGCAGGGTTTCC > 1/1472791‑1473253 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |