Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,472,624	G→A	T288I (ACT→ATT)	unknown ←	Transcription‑repair coupling factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,472,624	0	G	A	100.0%	195.1 / NA	69	T288I (ACT→ATT)	unknown	Transcription‑repair coupling factor
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (30/39);  total (30/39)

ATTGAATGGTCAACGGTGGCAGCGACGCAATCACTAAATTTTTCTGCCGCGCAGACGACCGCACTTTTTCCGCTTTTAGCGCAATAGCGGGATACTGTTTCAAGTGACGATTCACTTCATCAATGCGCAACCAAAGTTTATCGGGCGGTAACAACGGGCGCATAGGATCCACGCGACGACTTTCAAAACGCTGTTGTGCATCGGTGTAAAAACGCTCGCCTTGGTCTTGATTTTGCTCGGCGGTAGTAAACAGCGTATTTACCGGAAAATAATTGAACAAGGTTGCCATTTGCTCAAAAAACAGCGGCTGCCAATATTCAATGCCCGCAGCCAGCGTGCCTTTGCTGACCTGTTGATAAATGTGTTCCGGATCCCGTCGAATTTCCCCGAATTCCGCCCGAAATTGGCTACGGAAAAATTCAATACCCGTGCTATCCGTCGGAAATTCATGTGCCGGCAATAAATTAATTTGGTCGATTTCCTGC  >  1/1472379‑1472863                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                aTTGAATGGTCAACGGTGGCAGCGACGCAATCACTAAATTTTTCTGCCGCGCAGACGACCGCACTTTTTCCGCTTTTAGCGCAATAGCGGGATACTGTTTCAAGTGACGATTCACTTCATCAATGCGCAACCAAAGTTTATCGGGCGGTAACAACGGGCGCATAGGATCCACGCGACGACTTTCAAAACGCTGTTGTGCATCGGTGTAAAAACGCTCGCCTTGGGCTTGATTTTGCTCGGCGGTAATaaa                                                                                                                                                                                                                                             >  1:137290/1‑250 (MQ=255) aTTGAATGGTCAACGGTGGCAGCGACGCAATCACTAAATTTTTCTGCCGCGCAGACGACCGCACTTTTTCCGCTTTTAGCGCAATAGCGGGATACTGTTTCAAGTGACGATTCACTTCATCAATGCGCAACCAAAGTTTATCGGGCGGTAACAACGGGCGCATAGGATCCACGCGACGACTTTCAAAACGCTGTTGTGCATCGGTGTAAAAACGCTCGCCTTGGGCTTGATTTTGCTCGGCGGTAATAAAc                                                                                                                                                                                                                                            <  1:545798/251‑1 (MQ=255) aTTGAATGGTCAACGGTGGCAGCGACGCAATCACTAAATTTTTCTGCCGCGCAGACGACCGCACTTTTTCCGCTTTTAGCGCAATAGCGGGATACTGTTTCAAGTGACGATTCACTTCATCAATGCGCAACCAAAGTTTATCGGGCGGTAACAACGGGCGCATAGGATCCACGCGACGACTTTCAAAACGCTGTTGTGCATCGGTGTAAAAACGCTCGCCTTGGGCTTGATTTTGCTCGGCGGTAATAAAc                                                                                                                                                                                                                                            <  1:830780/251‑1 (MQ=255) aTTGAATGGTCAACGGTGGCAGCGACGCAATCACTAAATTTTTCTGCCGCGCAGACGACCGCACTTTTTCCGCTTTTAGCGCAATAGCGGGATACTGTTTCAAGTGACGATTCACTTCATCAATGCGCAACCAAAGTTTATCGGGCGGTAACAACGGGCGCATAGGATCCACGCGACGACTTTCAAAACGCTGTTGTGCATCGGTGTAAAAACGCTCGCCTTGGGCTTGATTTTGCTCGGCGGTAATAAAc                                                                                                                                                                                                                                            <  1:8026/251‑1 (MQ=255) aTTGAATGGTCAACGGTGGCAGCGACGCAATCACTAAATTTTTCTGCCGCGCAGACGACCGCACTTTTTCCGCTTTTAGCGCAATAGCGGGATACTGTTTCAAGTGACGATTCACTTCATCAATGCGCAACCAAAGTTTATCGGGCGGTAACAACGGGCGCATAGGATCCACGCGACGACTTTCAAAACGCTGTTGTGCATCGGTGTAAAAACGCTCGCCTTGGGCTTGATTTTGCTCGGCGGTAATAAAc                                                                                                                                                                                                                                            <  1:586051/251‑1 (MQ=255) aTTGAATGGTCAACGGTGGCAGCGACGCAATCACTAAATTTTTCTGCCGCGCAGACGACCGCACTTTTTCCGCTTTTAGCGCAATAGCGGGATACTGTTTCAAGTGACGATTCACTTCATCAATGCGCAACCAAAGTTTATCGGGCGGTAACAACGGGCGCATAGGATCCACGCGACGACTTTCAAAACGCTGTTGTGCATCGGTGTAAAAACGCTCGCCTTGGGCTTGATTTTGCTCGGCGGTAATAAAc                                                                                                                                                                                                                                            <  1:765487/251‑1 (MQ=255)    gAATGGTCAACGGTGGCAGCGACGCAATCACTAAATTTTTCTGCCGCGCAGACGACCGCACTTTTTCCGCTTTTAGCGCAATAGCGGGATACTGTTTCAAGTGACGATTCACTTCATCAATGCGCAACCAAAGTTTATCGGGCGGTAACAACGGGCGCATAGGATCCACGCGACGACTTTCAAAACGCTGTTGTGCATCGGTGTAAAAACGCTCGCCTTGGGCTTGATTTTGCTCGGCGGTAATAAAc                                                                                                                                                                                                                                            <  1:272325/248‑1 (MQ=255)    gAATGGTCAACGGTGGCAGCGACGCAATCACTAAATTTTTCTGCCGCGCAGACGACCGCACTTTTTCCGCTTTTAGCGCAATAGCGGGATACTGTTTCAAGTGACGATTCACTTCATCAATGCGCAACCAAAGTTTATCGGGCGGTAACAACGGGCGCATAGGATCCACGCGACGACTTTCAAAACGCTGTTGTGCATCGGTGTAAAAACGCTCGCCTTGGGCTTGATTTTGCTCGGCGGTAATAAAc                                                                                                                                                                                                                                            >  1:358604/1‑248 (MQ=255)      aTGGTCAACGGTGGCAGCGACGCAATCACTAAATTTTTCTGCCGCGCAGACGACCGCACTTTTTCCGCTTTTAGCGCAATAGCGGGATACTGTTTCAAGTGACGATTCACTTCATCAATGCGCAACCAAAGTTTATCGGGCGGTAACAACGGGCGCATAGGATCCACGCGACGACTTTCAAAACGCTGTTGTGCATCGGTGTAAAAACGCTCGCCTTGGGCTTGATTTTGCTCGGCGGTAATAAACAGCGt                                                                                                                                                                                                                                       <  1:871373/251‑1 (MQ=255)         gTCAACGGTGGCAGCGACGCAATCACTAAATTTTTCTGCCGCGCAGACGACCGCACTTTTTCCGCTTTTAGCGCAATAGCGGGATACTGTTTCAAGTGACGATTCACTTCATCAATGCGCAACCAAAGTTTATCGGGCGGTAACAACGGGCGCATAGGATCCACGCGACGACTTTCAAAACGCTGTTGTGCATCGGTGTAAAAACGCTCGCCTTGGGCTTGATTTTGCTCGGCGGTAATAAACAGCGTAtt                                                                                                                                                                                                                                    >  1:198742/1‑251 (MQ=255)            aaCGGTGGCAGCGACGCAATCACTAAATTTTTCTGCCGCGCAGACGACCGCACTTTTTCCGCTTTTAGCGCAATAGCGGGATACTGTTTCAAGTGACGATTCACTTCATCAATGCGCAACCAAAGTTTATCGGGCGGTAACAACGGGCGCATAGGATCCACGCGACGACTTTCAAAACGCTGTTGTGCATCGGTGTAAAAACGCTCGCCTTGGGCTTGATTTTGCTCGGCGGTAATAAACAGCGTATTTa                                                                                                                                                                                                                                  >  1:436074/1‑250 (MQ=255)            aaCGGTGGCAGCGACGCAATCACTAAATTTTTCTGCCGCGCAGACGACCGCACTTTTTCCGCTTTTAGCGCAATAGCGGGATACTGTTTCAAGTGACGATTCACTTCATCAATGCGCAACCAAAGTTTATCGGGCGGTAACAACGGGCGCATAGGATCCACGCGACGACTTTCAAAACGCTGTTGTGCATCGGTGTAAAAACGCTCGCCTTGGGCTTGATTTTGCTCGGCGGTAATAAACAGCGTATTTAc                                                                                                                                                                                                                                 <  1:153542/251‑1 (MQ=255)                       cGACGCAATCACTAAATTTTTCTGCCGCGCAGACGACCGCACTTTTTCCGCTTTTAGCGCAATAGCGGGATACTGTTTCAAGTGACGATTCACTTCATCAATGCGCAACCAAAGTTTATCGGGCGGTAACAACGGGCGCATAGGATCCACGCGACGACTTTCAAAACGCTGTTGTGCATCGGTGTAAAAACGCTCGCCTTGGGCTTGATTTTGCTCGGCGGTAATAAACAGCGTATTTACCGGAAaataat                                                                                                                                                                                                                      <  1:183182/251‑1 (MQ=255)                                      aTTTTTCTGCCGCGCAGACGACCGCACTTTTTCCGCTTTTAGCGCAATAGCGGGATACTGTTTCAAGTGACGATTCACTTCATCAATGCGCAACCAAAGTTTATCGGGCGGTAACAACGGGCGCATAGGATCCACGCGACGACTTTCAAAACGCTGTTGTGCATCGGTGTAAAAACGCTCGCCTTGGGCTTGATTTTGCTCGGCGGTAATAAACAGCGTATTTACCGGAAAATAATTGAACAAGGTTGCCa                                                                                                                                                                                                       >  1:649680/1‑251 (MQ=255)                                                   gcAGACGACCGCACTTTTTCCGCTTTTAGCGCAATAGCGGGATACTGTTTCAAGTGACGATTCACTTCATCAATGCGCAACCAAAGTTTATCGGGCGGTAACAACGGGCGCATAGGATCCACGCGACGACTTTCAAAACGCTGTTGTGCATCGGTGTAAAAACGCTCGCCTTGGGCTTGATTTTGCTCGGCGGTAATAAACAGCGTATTTACCGGAAAATAATTGAACAAGGTTGCCATTTGCTCaaaaaa                                                                                                                                                                                          >  1:61613/1‑251 (MQ=255)                                                   gcAGACGACCGCACTTTTTCCGCTTTTAGCGCAATAGCGGGATACTGTTTCAAGTGACGATTCACTTCATCAATGCGCAACCAAAGTTTATCGGGCGGTAACAACGGGCGCATAGGATCCACGCGACGACTTTCAAAACGCTGTTGTGCATCGGTGTAAAAACGCTCGCCTTGGGCTTGATTTTGCTCGGCGGTAATAAACAGCGTATTTACCGGAAAATAATTGAACAAGGTTGCCATTTGCTCaaaaaa                                                                                                                                                                                          >  1:526903/1‑251 (MQ=255)                                                       acgacCGCACTTTTTCCGCTTTTAGCGCAATAGCGGGATACTGTTTCAAGTGACGATTCACTTCATCAATGCGCAACCAAAGTTTATCGGGCGGTAACAACGGGCGCATAGGATCCACGCGACGACTTTCAAAACGCTGTTGTGCATCGGTGTAAAAACGCTCGCCTTGGGCTTGATTTTGCTCGGCGGTAATAAACAGCGTATTTACCGGAAAATAATTGAACAAGGTTGCCATTTGCTCAAAAAAc                                                                                                                                                                                         <  1:505390/248‑1 (MQ=255)                                                        cgacCGCACTTTTTCCGCTTTTAGCGCAATAGCGGGATACTGTTTCAAGTGACGATTCACTTCATCAATGCGCAACCAAAGTTTATCGGGCGGTAACAACGGGCGCATAGGATCCACGCGACGACTTTCAAAACGCTGTTGTGCATCGGTGTAAAAACGCTCGCCTTGGGCTTGATTTTGCTCGGCGGTAATAAACAGCGTATTTACCGGAAAATAATTGAACAAGGTTGCCATTTGCTCAAAAAACAGCg                                                                                                                                                                                     >  1:522322/1‑251 (MQ=255)                                                          acCGCACTTTTTCCGCTTTTAGCGCAATAGCGGGATACTGTTTCAAGTGACGATTCACTTCATCAATGCGCAACCAAAGTTTATCGGGCGGTAACAACGGGCGCATAGGATCCACGCGACGACTTTCAAAACGCTGTTGTGCATCGGTGTAAAAACGCTCGCCTTGGGCTTGATTTTGCTCGGCGGTAATAAAc                                                                                                                                                                                                                                            <  1:285974/194‑1 (MQ=255)                                                                   tttCCGCTTTTAGCGCAATAGCGGGATACTGTTTCAAGTGACGATTCACTTCATCAATGCGCAACCAAAGTTTATCGGGCGGTAACAACGGGCGCATAGGATCCACGCGACGACTTTCAAAACGCTGTTGTGCATCGGTGTAAAAACGCTCGCCTTGGGCTTGATTTTGCTCGGCGGTAATAAACAGCGTATTTACCGGAAAATAATTGAACAAGGTTGCCATTTGCTCAAAAAACAGCGGCTGCCAatat                                                                                                                                                                          <  1:234191/251‑1 (MQ=255)                                                                     tCCGCTTTTAGCGCAATAGCGGGATACTGTTTCAAGTGACGATTCACTTCATCAATGCGCAACCAAAGTTTATCGGGCGGTAACAACGGGCGCATAGGATCCACGCGACGACTTTCAAAACGCTGTTGTGCATCGGTGTAAAAACGCTCGCCTTGGGCTTGATTTTGCTCGGCGGTAATAAACAGCGTATTTACCGGAAAATAATTGAACAAGGTTGCCATTTGCTCAAAAAACAGCGGCTGCCAATATTc                                                                                                                                                                        <  1:632586/251‑1 (MQ=255)                                                                         cTTTTAGCGCAATAGCGGGATACTGTTTCAAGTGACGATTCACTTCATCAATGCGCAACCAAAGTTTATCGGGCGGTAACAACGGGCGCATAGGATCCACGCGACGACTTTCAAAACGCTGTTGTGCATCGGTGTAAAAACGCTCGCCTTGGGCTTGATTTTGCTCGGCGGTAATAAACAGCGt                                                                                                                                                                                                                                       >  1:704890/1‑184 (MQ=255)                                                                          ttttAGCGCAATAGCGGGATACTGTTTCAAGTGACGATTCACTTCATCAATGCGCAACCAAAGTTTATCGGGCGGTAACAACGGGCGCATAGGATCCACGCGACGACTTTCAAAACGCTGTTGTGCATCGGTGTAAAAACGCTCGCCTTGGGCTTGATTTTGCTCGGCGGTAATAAACAGCGTATTTACCGGAAAATAATTGAACAAGGTTGCCATTTGCTCAAAAAACAGCGGCTGCCAATATTCAATGc                                                                                                                                                                   <  1:594994/251‑1 (MQ=255)                                                                                  cAATAGCGGGATACTGTTTCAAGTGACGATTCACTTCATCAATGCGCAACCAAAGTTTATCGGGCGGTAACAACGGGCGCATAGGATCCACGCGACGACTTTCAAAACGCTGTTGTGCATCGGTGTAAAAACGCTCGCCTTGGGCTTGATTTTGCTCGGCGGTAATAAACAGCGTATTTACCGGAAaataat                                                                                                                                                                                                                      >  1:104828/1‑192 (MQ=255)                                                                                  cAATAGCGGGATACTGTTTCAAGTGACGATTCACTTCATCAATGCGCAACCAAAGTTTATCGGGCGGTAACAACGGGCGCATAGGATCCACGCGACGACTTTCAAAACGCTGTTGTGCATCGGTGTAAAAACGCTCGCCTTGGGCTTGATTTTGCTCGGCGGTAATAAACAGCGTATTTACCGGAAAATAATTGAACAAGGTTGCCATTTGCTCAAAAAACAGCGGCTGCCAATATTCAATGCCCGcagcc                                                                                                                                                           <  1:725690/251‑1 (MQ=255)                                                                                  cAATAGCGGGATACTGTTTCAAGTGACGATTCACTTCATCAATGCGCAACCAAAGTTTATCGGGCGGTAACAACGGGCGCATAGGATCCACGCGACGACTTTCAAAACGCTGTTGTGCATCGGTGTAAAAACGCTCGCCTTGGGCTTGATTTTGCTCGGCGGTAATAAACAGCGTATTTACCGGAAAATAATTGAACAAGGTTGCCATTTGCTCAAAAAACAGCGGCTGCCAATATTCAATGCCCGcagc                                                                                                                                                            >  1:750674/1‑250 (MQ=255)                                                                                       gCGGGATACTGTTTCAAGTGACGATTCACTTCATCAATGCGCAACCAAAGTTTATCGGGCGGTAACAACGGGCGCATAGGATCCACGCGACGACTTTCAAAACGCTGTTGTGCATCGGTGTAAAAACGCTCGCCTTGGGCTTGATTTTGCTCGGCGGTAATAAACAGCGTATTTACCGGAAAATAATTGAACAAGGTTGCCAtt                                                                                                                                                                                                     <  1:8395/204‑1 (MQ=255)                                                                                          ggATACTGTTTCAAGTGACGATTCACTTCATCAATGCGCAACCAAAGTTTATCGGGCGGTAACAACGGGCGCATAGGATCCACGCGACGACTTTCAAAACGCTGTTGTGCATCGGTGTAAAAACGCTCGCCTTGGGCTTGATTTTGCTCGGCGGTAATAAACAGCGTATTTACCGGAAAATAATTGAACAAGGTTGCCATTTGCTCAAAAAACAGCGGCTGCCAATATTCAATGCCCGCAGCCAGCGTGc                                                                                                                                                    >  1:37576/1‑250 (MQ=255)                                                                                            aTACTGTTTCAAGTGACGATTCACTTCATCAATGCGCAACCAAAGTTTATCGGGCGGTAACAACGGGCGCATAGGATCCACGCGACGACTTTCAAAACGCTGTTGTGCATCGGTGTAAAAACGCTCGCCTTGGGCTTGATTTTGCTCGGCGGTAATAAACAGCGTATTTACCGGAAAATAATTGAACAAGGTTGCCATTTGCTCAAAAAACAGCGGCTGCCAATATTCAATGCCCGCAGCCAGCGTGCCtt                                                                                                                                                 >  1:497313/1‑251 (MQ=255)                                                                                              aCTGTTTCAAGTGACGATTCACTTCATCAATGCGCAACCAAAGTTTATCGGGCGGTAACAACGGGCGCATAGGATCCACGCGACGACTTTCAAAACGCTGTTGTGCATCGGTGTAAAAACGCTCGCCTTGGGCTTGATTTTGCTCGGCGGTAATAAACAGCGTATTTACCGGAAAATAATTGAACAAGGTTGCCATTTGCTCAAAAAACAGCGGCTGCCAATATTCAATGc                                                                                                                                                                   <  1:901495/231‑1 (MQ=255)                                                                                               cTGTTTCAAGTGACGATTCACTTCATCAATGCGCAACCAAAGTTTATCGGGCGGTAACAACGGGCGCATAGGATCCACGCGACGACTTTCAAAACGCTGTTGTGCATCGGTGTAAAAACGCTCGCCTTGGGCTTGATTTTGCTCGGCGGTAATAAACAGCGTATTTACCGGAAAATAATTGAACAAGGTTGCCATTTGCTCAAAAAACAGCGGCTGCCAATATTCAATGCCCGCAGCCAGCGTGCCTTTg                                                                                                                                               >  1:212377/1‑250 (MQ=255)                                                                                                 gTTTCAAGTGACGATTCACTTCATCAATGCGCAACCAAAGTTTATCGGGCGGTAACAACGGGCGCATAGGATCCACGCGACGACTTTCAAAACGCTGTTGTGCATCGGTGTAAAAACGCTCGCCTTGGGCTTGATTTTGCTCGGCGGTAATAAACAGCGTATTTACCGGAAaataat                                                                                                                                                                                                                      <  1:604405/177‑1 (MQ=255)                                                                                                 gTTTCAAGTGACGATTCACTTCATCAATGCGCAACCAAAGTTTATCGGGCGGTAACAACGGGCGCATAGGATCCACGCGACGACTTTCAAAACGCTGTTGTGCATCGGTGTAAAAACGCTCGCCTTGGGCTTGATTTTGCTCGGCGGTAATAAACAGCGTATTTACCGGAAAATAATTGAACAAGGTTGCCATTTGCTCAAAAAACAGCGGCTGCCAATATTCAATGCCCGCAGCCAGCGTGCCTTTg                                                                                                                                               >  1:719508/1‑248 (MQ=255)                                                                                                  tttCAAGTGACGATTCACTTCATCAATGCGCAACCAAAGTTTATCGGGCGGTAACAACGGGCGCATAGGATCCACGCGACGACTTTCAAAACGCTGTTGTGCATCGGTGTAAAAACGCTCGCCTTGGGCTTGATTTTGCTCGGCGGTAATAAACAGCGTATTTACCGGAAAATAATTGAACAAGGTTGCCATTTGCTCAAAAAACAGCGGCTGCCAATATTCAATGCCCGCAGCCAGCGTGCCTTTGCTg                                                                                                                                            <  1:714379/250‑1 (MQ=255)                                                                                                  tttCAAGTGACGATTCACTTCATCAATGCGCAACCAAAGTTTATCGGGCGGTAACAACGGGCGCATAGGATCCACGCGACGACTTTCAAAACGCTGTTGTGCATCGGTGTAAAAACGCTCGCCTTGGGCTTGATTTTGCTCGGCGGTAATAAACAGCGTATTTACCGGAAAATAATTGAACAAGGTTGCCATTTGCTCAAAAAACAGCGGCTGCCAATATTCAATGCCCGCAGCCAGCGTGCCTTTGCTg                                                                                                                                            <  1:236026/250‑1 (MQ=255)                                                                                                              aTTCACTTCATCAATGCGCAACCAAAGTTTATCGGGCGGTAACAACGGGCGCATAGGATCCACGCGACGACTTTCAAAACGCTGTTGTGCATCGGTGTAAAAACGCTCGCCTTGGGCTTGATTTTGCTCGGCGGTAATAAACAGCGTATTTACCGGAAAATAATTGAACAAGGTTGCCATTTGCTCAAAAAACAGCGGCTGCCAATATTCAATGCCCGCAGCCAGCGTGCCTTTGCTGACCTGTTGATaaa                                                                                                                               <  1:317913/251‑1 (MQ=255)                                                                                                              aTTCACTTCATCAATGCGCAACCAAAGTTTATCGGGCGGTAACAACGGGCGCATAGGATCCACGCGACGACTTTCAAAACGCTGTTGTGCATCGGTGTAAAAACGCTCGCCTTGGGCTTGATTTTGCTCGGCGGTAATAAACAGCGTATTTACCGGAAAATAATTGAACAAGGTTGCCATTTGCTCAAAAAACAGCGGCTGCCAATATTCAATGCCCGCAGCCAGCGTGCCTTTGCTGACCTGTTGATaaa                                                                                                                               <  1:546969/251‑1 (MQ=255)                                                                                                                tCACTTCATCAATGCGCAACCAAAGTTTATCGGGCGGTAACAACGGGCGCATAGGATCCACGCGACGACTTTCAAAACGCTGTTGTGCATCGGTGTAAAAACGCTCGCCTTGGGCTTGATTTTGCTCGGCGGTAATAAACAGCGTATTTACCGGAAAATAATTGAACAAGGTTGCCATTTGCTCAAAAAACAGCGGCTGCCAATATTCAATGCCCGCAGCCAGCGTGCCTTTGCTGACCTGTTGATAAAtg                                                                                                                             <  1:706786/251‑1 (MQ=255)                                                                                                                   cTTCATCAATGCGCAACCAAAGTTTATCGGGCGGTAACAACGGGCGCATAGGATCCACGCGACGACTTTCAAAACGCTGTTGTGCATCGGTGTAAAAACGCTCGCCTTGGGCTTGATTTTGCTCGGCGGTAATAAACAGCGTATTTAc                                                                                                                                                                                                                                 <  1:146177/148‑1 (MQ=255)                                                                                                                   cTTCATCAATGCGCAACCAAAGTTTATCGGGCGGTAACAACGGGCGCATAGGATCCACGCGACGACTTTCAAAACGCTGTTGTGCATCGGTGTAAAAACGCTCGCCTTGGGCTTGATTTTGCTCGGCGGTAATAAACAGCGTATTTACCGGAAAATAATTGAACAAGGTTGCCATTTGCTCAAAAAACAGCGGCTGcc                                                                                                                                                                               >  1:233758/1‑198 (MQ=255)                                                                                                                     tcatcaATGCGCAACCAAAGTTTATCGGGCGGTAACAACGGGCGCATAGGATCCACGCGACGACTTTCAAAACGCTGTTGTGCATCGGTGTAAAAACGCTCGCCTTGGGCTTGATTTTGCTCGGCGGTAATAAACAGCGTATTTACCGGAAAATAATTGAACAAGGTTGCCATTTGCTCAAAAAACAGCGGCTGCCAATATTCAATGCCCGcagc                                                                                                                                                            <  1:866171/215‑1 (MQ=255)                                                                                                                        tcaATGCGCAACCAAAGTTTATCGGGCGGTAACAACGGGCGCATAGGATCCACGCGACGACTTTCAAAACGCTGTTGTGCATCGGTGTAAAAACGCTCGCCTTGGGCTTGATTTTGCTCGGCGGTAATAAAc                                                                                                                                                                                                                                            <  1:185522/132‑1 (MQ=255)                                                                                                                          aaTGCGCAACCAAAGTTTATCGGGCGGTAACAACGGGCGCATAGGATCCACGCGACGACTTTCAAAACGCTGTTGTGCATCGGTGTAAAAACGCTCGCCTTGGGCTTGATTTTGCTCGGCGGTAATAAACAGCGTATTTACCGGAAAATAATTGAACAAGGTTGCCATTTGCTCAAAAAACAGCGGCTGCCAATATTCAATGCCCGCAGCCAGCGTGCCTTTGCTGACCTGTTGATAAATGTGTTCCGGAt                                                                                                                   <  1:370321/251‑1 (MQ=255)                                                                                                                            tGCGCAACCAAAGTTTATCGGGCGGTAACAACGGGCGCATAGGATCCACGCGACGACTTTCAAAACGCTGTTGTGCATCGGTGTAAAAACGCTCGCCTTGGGCTTGATTTTGCTCGGCGGTAATAAACAGCGTATTTACCGGAAAATAATTGAACAAGGTTGCCATTTGCTCAAAAAACAGCGGCTGCCAATATTCAATGCCCGCAGCCAGCGTGCCTTTGCTGACCTGTTGATAAATGTGTTCCGGATcc                                                                                                                 <  1:183042/251‑1 (MQ=255)                                                                                                                                      aaGTTTATCGGGCGGTAACAACGGGCGCATAGGATCCACGCGACGACTTTCAAAACGCTGTTGTGCATCGGTGTAAAAACGCTCGCCTTGGGCTTGATTTTGCTCGGCGGTAATAAACAGCGTATTTACCGGAAAATAATTGAACAAGGTTGCCATTTGCTCAAAAAACAGCGGCTGCCAATATTCAATGCCCGCAGCCAGCGTGCCTTTGCTGAcc                                                                                                                                         >  1:894355/1‑217 (MQ=255)                                                                                                                                                  cGGTAACAACGGGCGCATAGGATCCACGCGACGACTTTCAAAACGCTGTTGTGCATCGGTGTAAAAACGCTCGCCTTGGGCTTGATTTTGCTCGGCGGTAATAAACAGCGTATTTACCGGAAAATAATTGAACAAGGTTGCCATTTGCTCAAAAAAc                                                                                                                                                                                         <  1:684342/157‑1 (MQ=255)                                                                                                                                                      aacaacGGGCGCATAGGATCCACGCGACGACTTTCAAAACGCTGTTGTGCATCGGTGTAAAAACGCTCGCCTTGGGCTTGATTTTGCTCGGCGGTAATAAACAGCGTATTTACCGGAAAATAATTGAACAAGGTTGCCATTTGCTCAAAAAACAGCGGCTGCCAATATTCAATGCCCGCAGCCAGCGTGCCTTTGCTGACCTGTTGATaaa                                                                                                                               <  1:622465/211‑1 (MQ=255)                                                                                                                                                         aacGGGCGCATAGGATCCACGCGACGACTTTCAAAACGCTGTTGTGCATCGGTGTAAAAACGCTCGCCTTGGGCTTGATTTTGCTCGGCGGTAATAAACAGCGTATTTACCGGAAAATAATTGAACAAGGTTGCCATTTGCTCAAAAAACAGCGGCTGCCAATATTCAATGCCCGCAGCCAGCGTGCCTTTGCTGACCTGTTGATAAATGTGTTCCGGATCCCGTCGAATTTCCCCGAATTCCGCCCGaaa                                                                                    <  1:232758/251‑1 (MQ=255)                                                                                                                                                          acGGGCGCATAGGATCCACGCGACGACTTTCAAAACGCTGTTGTGCATCGGTGTAAAAACGCTCGCCTTGGGCTTGATTTTGCTCGGCGGTAATAAACAGCGTATTTACCGGAAAATAATTGAACAAGGTTGCCATTTGCTCAAAAAACAGCGGCTGCCAATATTCAATGCCCGCAGCCAGCGTGCCTTTGCTGACCTGTTGATAAATGTGTTCCGGATCCCGTCGAATTTCCCCGAATTCCGCCCGAAAt                                                                                   <  1:412985/251‑1 (MQ=255)                                                                                                                                                            gggCGCATAGGATCCACGCGACGACTTTCAAAACGCTGTTGTGCATCGGTGTAAAAACGCTCGCCTTGGGCTTGATTTTGCTCGGCGGTAATAAACAGCGTATTTACCGGAAAATAATTGAACAAGGTTGCCATTTGCTCAAAAAACAGCGGCTGCCAATATTCAATGcc                                                                                                                                                                  <  1:459443/170‑1 (MQ=255)                                                                                                                                                             ggCGCATAGGATCCACGCGACGACTTTCAAAACGCTGTTGTGCATCGGTGTAAAAACGCTCGCCTTGGGCTTGATTTTGCTCGGCGGTAATAAACAGCGTATTTACCGGAAAATAATTGAACAAGGTTGCCATTTGCTCAAAAAACAGCGGCTGCCAATATTCAATGCCCGCAGCCAGCGTGCCTTTGCTGACCTGTTGATAAATGTGTTCCGGATCCCGTCGAATTTCCCCGAATTCCGCCCGAAATTgg                                                                                >  1:267099/1‑251 (MQ=255)                                                                                                                                                                 cATAGGATCCACGCGACGACTTTCAAAACGCTGTTGTGCATCGGTGTAAAAACGCTCGCCTTGGGCTTGATTTTGCTCGGCGGTAATAAACAGCGTATTTACCGGAAAATAATTGAACAAGGTTGCCATTTGCTCAAAAAACAGCGGCTGCCAATATTCAATGCCCGCAGCCAGCGTGCCTTTGCTGACCTGTTGATAAATGTGTTCCGGATCCCGTCGAATTTCCCCGAATTCCGCCCGAAATTGGCt                                                                              >  1:26194/1‑249 (MQ=255)                                                                                                                                                                                     tttCAAAACGCTGTTGTGCATCGGTGTAAAAACGCTCGCCTTGGGCTTGATTTTGCTCGGCGGTAATAAACAGCGTATTTACCGGAAAATAATTGAACAAGGTTGCCATTTGCTCAAAAAACAGCGGCTGCCAATATTCAATGCCCGCAGCCAGCGTGCCTTTGCTGACCTGTTGATAAATGTGTTCCGGATCCCGTCGAATTTCCCCGAATTCCGCCCGAAATTGGCTACGGAAAAATTCAATACCCGTg                                                        >  1:780242/1‑251 (MQ=255)                                                                                                                                                                                               cTGTTGTGCATCGGTGTAAAAACGCTCGCCTTGGGCTTGATTTTGCTCGGCGGTAATAAACAGCGTATTTACCGGAAAATAATTGAACAAGGTTGCCATTTGCTCAAAAAACAGCGGCTGCCAATATTCAATGCCCGCAGCCAGCGTGCCTTTGCTGACCTGTTGATaaa                                                                                                                               >  1:240075/1‑170 (MQ=255)                                                                                                                                                                                               cTGTTGTGCATCGGTGTAAAAACGCTCGCCTTGGGCTTGATTTTGCTCGGCGGTAATAAACAGCGTATTTACCGGAAAATAATTGAACAAGGTTGCCATTTGCTCAAAAAACAGCGGCTGCCAATATTCAATGCCCGCAGCCAGCGTGCCTTTGCTGACCTGTTGATAAATGTGTTCCGGATCCCGTCGAATTTCCCCGAATTCCGCCCGAAATTGGCTACGGAAAAATTc                                                                  <  1:691001/231‑1 (MQ=255)                                                                                                                                                                                               cTGTTGTGCATCGGTGTAAAAACGCTCGCCTTGGGCTTGATTTTGCTCGGCGGTAATAAACAGCGTATTTACCGGAAAATAATTGAACAAGGTTGCCATTTGCTCAAAAAACAGCGGCTGCCAATATTCAATGCCCGCAGCCAGCGTGCCTTTGCTGACCTGTTGATAAATGTGTTCCGGATCCCGTCGAATTTCCCCGAATTCCGCCCGAAATTGGCTACGGAAAAATTc                                                                  >  1:213304/1‑231 (MQ=255)                                                                                                                                                                                                 gttgtGCATCGGTGTAAAAACGCTCGCCTTGGGCTTGATTTTGCTCGGCGGTAATAAACAGCGTATTTACCGGAAAATAATTGAACAAGGTTGCCATTTGCTCAAAAAACAGCGGCTGCCAATATTCAATGCCCGCAGCCAGCGTGCCTTTGCTGACCTGTTGATAAATGTGTTCCGGATCCCGTCGAATTTCCCCGAATTCCGCCCGAAATTGGCTACGGAAAAATTc                                                                  >  1:880400/1‑229 (MQ=255)                                                                                                                                                                                                 gttgtGCATCGGTGTAAAAACGCTCGCCTTGGGCTTGATTTTGCTCGGCGGTAATAAACAGCGTATTTACCGGAAAATAATTGAACAAGGTTGCCATTTGCTCAAAAAACAGCGGCTGCCAATATTCAATGCCCGCAGCCAGCGTGCCTTTGCTGACCTGTTGATAAATGTGTTCCGGATCCCGTCGAATTTCCCCGAATTCCGCCCGAAATTGGCTACGGAAAAATTCAATACCCGTGCTATCCGTCGGa                                            >  1:457468/1‑251 (MQ=255)                                                                                                                                                                                                           ggTGTAAAAACGCTCGCCTTGGGCTTGATTTTGCTCGGCGGTAATAAACAGCGTATTTACCGGAAAATAATTGAACAAGGTTGCCATTTGCTCAAAAAACAGCGGCTGCCAATATTCAATGCCCGCAGCCAGCGTGCCTTTGCTGACCTGTTGATAAATGTGTTCCGGATCCCGTCGAATTTCCCCGAATTCCGCCCGAAATTGGCTACGGAAAAATTCAATACCCGTGCTATCCGTCGGAAATTCAtgtg                                  >  1:566027/1‑251 (MQ=255)                                                                                                                                                                                                            gtgtAAAAACGCTCGCCTTGGGCTTGATTTTGCTCGGCGGTAATAAACAGCGTATTTACCGGAAAATAATTGAACAAGGTTGCCATTTGCTCAAAAAACAGCGGCTGCCAATATTCAATGCCCGCAGCCAGCGTGCCTTTGCTGACCTGTTGATAAATGTGTTCCGGATCCCGTCGAATTTCCCCGAATTCCGCCCGAAATTGGCTACGGAAAAATTCAATAcc                                                            >  1:179449/1‑224 (MQ=255)                                                                                                                                                                                                            gtgtAAAAACGCTCGCCTTGGGCTTGATTTTGCTCGGCGGTAATAAACAGCGTATTTACCGGAAAATAATTGAACAAGGTTGCCATTTGCTCAAAAAACAGCGGCTGCCAATATTCAATGCCCGCAGCCAGCGTGCCTTTGCTGACCTGTTGATAAATGTGTTCCGGATCCCGTCGAATTTCCCCGAATTCCGCCCGAAATTGGCTACGGAAAAATTCAATACCCGTGCTa                                                     <  1:791186/231‑1 (MQ=255)                                                                                                                                                                                                            gtgtAAAAACGCTCGCCTTGGGCTTGATTTTGCTCGGCGGTAATAAACAGCGTATTTACCGGAAAATAATTGAACAAGGTTGCCATTTGCTCAAAAAACAGCGGCTGCCAATATTCAATGCCCGCAGCCAGCGTGCCTTTGCTGACCTGTTGATAAATGTGTTCCGGATCCCGTCGAATTTCCCCGAATTCCGCCCGAAATTGGCTACGGAAAAATTCAATACCCGTGCTATCCGTCGGAAATTCAtgtg                                  >  1:391504/1‑250 (MQ=255)                                                                                                                                                                                                            gtgtAAAAACGCTCGCCTTGGGCTTGATTTTGCTCGGCGGTAATAAACAGCGTATTTACCGGAAAATAATTGAAC‑AGGTTGCCATTTGCTCAAAAAACAGCGGCTGCCAATATTc                                                                                                                                                                        >  1:459771/1‑115 (MQ=255)                                                                                                                                                                                                                       cTCGCCTTGGGCTTGATTTTGCTCGGCGGTAATAAACAGCGTATTTACCGGAAAATAATTGAACAAGGTTGCCATTTGCTCAAAAAACAGCGGCTGCCAATATTCAATGCCCGCAGCCAGCGTGCCTTTGCTGACCTGTTGATAAATGTGTTCCGGATCCCGTCGAATTTCCCCGAATTCCGCCCGAAATTGGCTACGGAAAAATTCAATACCCGTGCTATCCGTCGGAAATTCATGTGCCGGCAATAaat                      <  1:679804/251‑1 (MQ=255)                                                                                                                                                                                                                           ccTTGGGCTTGATTTTGCTCGGCGGTAATAAACAGCGTATTTACCGGAAAATAATTGAACAAGGTTGCCATTTGCTCAAAAAACAGCGGCTGCCAATATTCAATGCCCGCAGCCAGCGTGCCTTTGCTGACCTGTTGATAAATGTGTTCCGGATCCCGTCGAATTTCCCCGAATTCCGCCCGAAATTGGCTACGGAAAAATTCAATACCCGTGCTATCCGTCGGAAATTCATGTGCCGGCAATAaattgat                  >  1:245689/1‑248 (MQ=255)                                                                                                                                                                                                                            cTTGGGCTTGATTTTGCTCGGCGGTAATAAACAGCGTATTTACCGGAAAATAATTGAACAAGGTTGCCATTTGCTCAAAAAACAGCGGCTGCCAATATTCAATGCCCGCAGCCAGCGTGCCTTTGCTGACCTGTTGATAAATGTGTTCCGGATCCCGTCGAATTTCCCCGAATTCCGCCCGAAATTGGCTACGGAAAAATTCAATACCCGTGCTATCCGTCGGAAATTCATGTGCCGGCAATAaattgatt                 <  1:788486/251‑1 (MQ=255)                                                                                                                                                                                                                            cTTGGGCTTGATTTTGCTCGGCGGTAATAAACAGCGTATTTACCGGAAAATAATTGAACAAGGTTGCCATTTGCTCAAAAAACAGCGGCTGCCAATATTCAATGCCCGCAGCCAGCGTGCCTTTGCTGACCTGTTGATAAATGTGTTCCGGATCCCGTCGAATTTCCCCGAATTCCGCCCGAAATTGGCTACGGAAAAATTCAATACCCGTGCTATCCGTCGGAAATTCATGTGCCGGCAATAaattgatt                 <  1:554248/251‑1 (MQ=255)                                                                                                                                                                                                                               gggcTTGATTTTGCTCGGCGGTAATAAACAGCGTATTTACCGGAAAATAATTGAACAAGGTTGCCATTTGCTCAAAAAACAGCGGCTGCCAATATTCAATGCCCGCAGCCAGCGTGCCTTTGCTGACCTGTTGATAAATGTGTTCCGGATCCCGTCGAATTTCCCCGAATTCCGCCCGAAATTGGCTACGGAAAAATTc                                                                  >  1:496451/4‑199 (MQ=255)                                                                                                                                                                                                                                            cTCGGCGGTAATAAACAGCGTATTTACCGGAAAATAATTGAACAAGGTTGCCATTTGCTCAAAAAACAGCGGCTGCCAATATTCAATGCCCGCAGCCAGCGTGCCTTTGCTGACCTGTTGATAAATGTGTTCCGGATCCCGTCGAATTTCCCCGAATTCCGCCCGAAATTGGCTACGGAAAAATTCAATACCCGTGCTATCCGTCGGAAATTCATGTGCCGGCAATAAATTGATTTGGTCGATTTCCTGc  <  1:46354/250‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                ATTGAATGGTCAACGGTGGCAGCGACGCAATCACTAAATTTTTCTGCCGCGCAGACGACCGCACTTTTTCCGCTTTTAGCGCAATAGCGGGATACTGTTTCAAGTGACGATTCACTTCATCAATGCGCAACCAAAGTTTATCGGGCGGTAACAACGGGCGCATAGGATCCACGCGACGACTTTCAAAACGCTGTTGTGCATCGGTGTAAAAACGCTCGCCTTGGTCTTGATTTTGCTCGGCGGTAGTAAACAGCGTATTTACCGGAAAATAATTGAACAAGGTTGCCATTTGCTCAAAAAACAGCGGCTGCCAATATTCAATGCCCGCAGCCAGCGTGCCTTTGCTGACCTGTTGATAAATGTGTTCCGGATCCCGTCGAATTTCCCCGAATTCCGCCCGAAATTGGCTACGGAAAAATTCAATACCCGTGCTATCCGTCGGAAATTCATGTGCCGGCAATAAATTAATTTGGTCGATTTCCTGC  >  1/1472379‑1472863

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑