| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | 1 | 1,456,669 | T→G | A249A (GCA→GCC) | unknown ← | Phospho‑N‑acetylmuramoyl‑pentapeptide‑ transferase (EC 2.7.8.13) |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | 1 | 1,456,669 | 0 | T | G | 98.8% | 226.1 / NA | 84 | A249A (GCA→GCC) | unknown | Phospho‑N‑acetylmuramoyl‑pentapeptide‑ transferase (EC 2.7.8.13) |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base G (54/29); minor base A (0/1); total (54/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.57e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
CGAAATGGTGATGAATCGGCGCCATCAGGAAAATACGTTGTTTATTACGCAGTTTGTAGGAGCCCACTTGTAAAATGACGGACAGGGTTTCCATGACAAACACGCCGCCCATAATTAATAATAAAAATTCCTGACGCACTAACACGGCAATCACGCCCAGCGCACCGCCTAATGCTAATGAGCCTACATCGCCCATAAACACCTGCGCAGGATAAGTATTGAACCAAAGAAAACCCAAACCTGCACCCACAATGGCGGTACAGAACACCACTAATTCCGCACTGAATTTAATATACGGAATGTGTAAATATTCGGCGAAATTAATGTTCCCCGTCGCCCATGCCACCAAGGCAAATGCACCGGCAACAAGCACCGTCGGCATAATTGCCAAGCCGTCCAAGCCGTCGGTGAAATTCACCGCGTTACTGGAGCCGACGATAACGAAATAAGACAGCACGATATAAAAGACACCGAGTTGTGGCA > 1/1456428‑1456910 | cGAAATGGTGATGAATCGGCGCCATCAGGAAAATACGTTGTTTATTACGCAGTTTGTAGGAGCCCACTTGTAAAATGACGGACAGGGTTTCCATGACAAACACGCCGCCCATAATTAATAATAAAAATTCCTGACGCACTAACACGGCAATCACGCCCAGCGCACCGCCTAATGCTAATGAGCATACATCGCCCATAAACACCTGCGCAGGATAAGTATTGAACCAAAGAAAACCCAAACCGGCACCcaca < 1:623603/251‑1 (MQ=255) aTGGTGATGAATCGGCGCCATCAGGAAAATACGTTGTTTATTACGCAGTTTGTAGGAGCCCACTTGTAAAATGACGGACAGGGTTTCCATGACAAACACGCCGCCCATAATTAATAATAAAAATTCCTGACGCACTAACACGGCAATCACGCCCAGCGCACCGCCTAATGCTAATGAGCCTACATCGCCCATAAACACCTGCGCAGGATAAGTATTGAACCAAAGAAAACCCAAACCGGCACCCACAATgg < 1:440610/251‑1 (MQ=255) gTGATGAATCGGCGCCATCAGGAAAATACGTTGTTTATTACGCAGTTTGTAGGAGCCCACTTGTAAAATGACGGACAGGGTTTCCATGACAAACACGCCGCCCATAATTAATAATAAAAATTCCTGACGCACTAACACGGCAATCACGCCCAGCGCACCGCCTAATGCTAATGAGCCTACATCGCCCATAAACACCTGCGCAGGATAAGTATTGAACCAAAGAAAACCCAAACCGGCACCCACAATGGCgg > 1:384405/1‑251 (MQ=255) gTGATGAATCGGCGCCATCAGGAAAATACGTTGTTTATTACGCAGTTTGTAGGAGCCCACTTGTAAAATGACGGACAGGGTTTCCATGACAAACACGCCGCCCATAATTAATAATAAAAATTCCTGACGCACTAACACGGCAATCACGCCCAGCGCACCGCCTAATGCTAATGAGCCTACATCGCCCATAAACACCTGCGCAGGATAAGTATTGAACCAAAGAAAACCCAAACCGGCACCCACAATGGCgg > 1:700097/1‑251 (MQ=255) tCGGCGCCATCAGGAAAATACGTTGTTTATTACGCAGTTTGTAGGAGCCCACTTGTAAAATGACGGACAGGGTTTCCATGACAAACACGCCGCCCATAATTAATAATAAAAATTCCTGACGCACTAACACGGCAATCACGCCCAGCGCACCGCCTAATGCTAATGAGCCTACATCGCCCATAAACACCTGCGCAGGATAAGTATTGAACCAAAGAAAACCCAAACCGGCACCCACAATGGCGGTACAGAac < 1:31323/251‑1 (MQ=255) cAGGAAAATACGTTGTTTATTACGCAGTTTGTAGGAGCCCACTTGTAAAATGACGGACAGGGTTTCCATGACAAACACGCCGCCCATAATTAATAATAAAAATTCCTGACGCACTAACACGGCAATCACGCCCAGCGCACCGCCTAATGCTAATGAGCCTACATCGCCCATAAACACCTGCGCAGGATAAGTATTGAACCAAAGAAAACCCAAACCGGCACCCACAATGGCGGTAc < 1:323107/236‑1 (MQ=255) cAGGAAAATACGTTGTTTATTACGCAGTTTGTAGGAGCCCACTTGTAAAATGACGGACAGGGTTTCCATGACAAACACGCCGCCCATAATTAATAATAAAAATTCCTGACGCACTAACACGGCAATCACGCCCAGCGCACCGCCTAATGCTAATGAGCCTACATCGCCCATAAACACCTGCGCAGGATAAGTATTGAACCAAAGAAAACCCAAACCGGCACCCACAATGGCGGTACAGAACACCACTAAtt > 1:568977/1‑251 (MQ=255) gAAAATACGTTGTTTATTACGCAGTTTGTAGGAGCCCACTTGTAAAATGACGGACAGGGTTTCCATGACAAACACGCCGTCCATAATTAATAATAAAAATTCCTGACGCACTAACACGGCAATCACGCCCAGCGCACCGCCTAATGCTAATGAGCCTACATCGCCCATAAACACCTGCGCAGGATAAGTATTGAACCAAAGAAAACCCAAACCGGCACCCACAATGGCGGTACAGAACACCACTAATTc < 1:430429/249‑1 (MQ=255) gAAAATACGTTGTTTATTACGCAGTTTGTAGGAGCCCACTTGTAAAATGACGGACAGGGTTTCCATGACAAACACGCCGCCCATAATTAATAATAAAAATTCCTGACGCACTAACACGGCAATCACGCCCAGCGCACCGCCTAATGCTAATGAGCCTACATCGCCCATAAACACCTGCGCAGGATAAGTATTGAACCAAAGAAAACCCAAACCGGCACCCACAATGGCGGTACAGAACACCACTAAtt < 1:884770/248‑1 (MQ=255) gAAAATACGTTGTTTATTACGCAGTTTGTAGGAGCCCACTTGTAAAATGACGGACAGGGTTTCCATGACAAACACGCCGCCCATAATTAATAATAAAAATTCCTGACGCACTAACACGGCAATCACGCCCAGCGCACCGCCTAATGCTAATGAGCCTACATCGCCCATAAACACCTGCGCAGGATAAGTATTGAACCAAAGAAAACCCAAACCGGCACCCACAATGGCGGTACAGAACACCACTAATTc < 1:457108/249‑1 (MQ=255) gAAAATACGTTGTTTATTACGCAGTTTGTAGGAGCCCACTTGTAAAATGACGGACAGGGTTTCCATGACAAACACGCCGCCCATAATTAATAATAAAAATTCCTGACGCACTAACACGGCAATCACGCCCAGCGCACCGCCTAATGCTAATGAGCCTACATCGCCCATAAACACCTGCGCAGGATAAGTATTGAACCAAAGAAAACCCAAACCGGCACCCACAATGGCGGTACAGAACACCACTAATTc < 1:340562/249‑1 (MQ=255) gttTATTACGCAGTTTGTAGGAGCCCACTTGTAAAATGACGGACAGGGTTTCCATGACAAACACGCCGCCCATAATTAATAATAAAAATTCCTGACGCACTAACACGGCAATCACGCCCAGCGCACCGCCTAATGCTAATGAGCCTACATCGCCCATAAACACCTGCGCAGGATAAGTATTGAACCAAAGAAAACCCAAACCGGCACCCACAATGGCGGTACAGAAcaccac > 1:567213/1‑232 (MQ=255) gttTATTACGCAGTTTGTAGGAGCCCACTTGTAAAATGACGGACAGGGTTTCCATGACAAACACGCCGCCCATAATTAATAATAAAAATTCCTGACGCACTAACACGGCAATCACGCCCAGCGCACCGCCTAATGCTAATGAGCCTACATCGCCCATAAACACCTGCGCAGGATAAGTATTGAACCAAAGAAAACCCAAACCGGCACCCACAATGGCGGTACAGAACACCACTAATTCCGCACTGAATTTa > 1:38788/1‑251 (MQ=255) ttattaCGCAGTTTGTAGGAGCCCACTTGTAAAATGACGGACAGGGTTTCCATGACAAACACGCCGCCCATAATTAATAATAAAAATTCCTGACGCACTAACACGGCAATCACGCCCAGCGCACCGCCTAATGCTAATGAGCCTACATCGCCCATAAACACCTGCGCAGGATAAGTATTGAACCAAAGAAAACCCAAACCGGCACCCACAATGGCGGTACAGAac > 1:482744/1‑225 (MQ=255) ttattaCGCAGTTTGTAGGAGCCCACTTGTAAAATGACGGACAGGGTTTCCATGACAAACACGCCGCCCATAATTAATAATAAAAATTCCTGACGCACTAACACGGCAATCACGCCCAGCGCACCGCCTAATGCTAATGAGCCTACATCGCCCATAAACACCTGCGCAGGATAAGTATTGAACCAAAGAAAACCCAAACCGGCACCCACAATGGCGGTACAGAACACCACTAATTCCGCACTGAATTTAat > 1:609647/1‑251 (MQ=255) ttattaCGCAGTTTGTAGGAGCCCACTTGTAAAATGACGGACAGGGTTTCCATGACAAACACGCCGCCCATAATTAATAATAAAAATTCCTGACGCACTAACACGGCAATCACGCCCAGCGCACCGCCTAATGCTAATGAGCCTACATCGCCCATAAACACCTGCGCAGGATAAGTATTGAACCAAAGAAAACCCAAACCGGCACCCACAATGGCGGTACAGAACACCACTAATTCCGCACTGAATTTAat > 1:829737/1‑251 (MQ=255) attaCGCAGTTTGTAGGAGCCCACTTGTAAAATGACGGACAGGGTTTCCATGACAAACACGCCGCCCATAATTAATAATAAAAATTCCTGACGCACTAACACGGCAATCACGCCCAGCGCACCGCCTAATGCTAATGAGCCTACATCGCCCATAAACACCTGCGCAGGATAAGTATTGAACCAAAGAAAACCCAAACCGGCACCCACAATGGCGGTACAGAACACCACTAATTCCGCACTGAATTTAatat > 1:305440/1‑251 (MQ=255) aCGCAGTTTGTAGGAG‑CCACTTGTAAAATGACGGACAGGGTTTCCATGACAAACACGCCGCCCATAATTAATAATAAAAATTCCTGACGCACTAACACGGCAATCACGCCCAGCGCACCGCCTAATGCTAATGAGCCTACATCGCCCATAAACACCTGCGCAGGATAAGTATTGAACCAAAGAAAACCCAAACCGGCACCCACAATGGCGGTACAGAACACCACTAATTCCGCACTGAATTTAATATACgg < 1:606312/251‑1 (MQ=255) gTTTGTAGGAGCCCACTTGTAAAATGACGGACAGGGTTTCCATGACAAACACGCCGCCCATAATTAATAATAAAAATTCCTGACGCACTAACACGGCAATCACGCCCAGCGCACCGCCTAATGCTAATGAGCCTACATCGCCCATAAACACCTGCGCAGGATAAGTATTGAACCAAAGAAAACCCAAACCGGCACCCACAATGGCGGTAc < 1:161122/210‑1 (MQ=255) gTTTGTAGGAGCCCACTTGTAAAATGACGGACAGGGTTTCCATGACAAACACGCCGCCCATAATTAATAATAAAAATTCCTGACGCACTAACACGGCAATCACGCCCAGCGCACCGCCTAATGCTAATGAGCCTACATCGCCCATAAACACCTGCGCAGGATAAGTATTGAACCAAAGAAAACCCAAACCGGCACCCACAATGGCGGTACAGAac < 1:259781/215‑1 (MQ=255) gTTTGTAGGAGCCCACTTGTAAAATGACGGACAGGGTTTCCATGACAAACACGCCGCCCATAATTAATAATAAAAATTCCTGACGCACTAACACGGCAATCACGCCCAGCGCACCGCCTAATGCTAATGAGCCTACATCGCCCATAAACACCTGCGCAGGATAAGTATTGAACCAAAGAAAACCCAAACCGGCACCCACAATGGCGGTACAGAACACCACTAATTCCGCACTGAATTTAATATACGGAAtg > 1:736725/1‑251 (MQ=255) gTAGGAGCCCACTTGTAAAATGACGGACAGGGTTTCCATGACAAACACGCCGCCCATAATTAATAATAAAAATTCCTGACGCACTAACACGGCAATCACGCCCAGCGCACCGCCTAATGCTAATGAGCCTACATCGCCCATAAACACCTGCGCAGGATAAGTATTGAACCAAAGAAAACCCAAACCGGCACCCACAATGGCGGTACAg > 1:629837/1‑208 (MQ=255) gTAGGAGCCCACTTGTAAAATGACGGACAGGGTTTCCATGACAAACACGCCGCCCATAATTAATAATAAAAATTCCTGACGCACTAACACGGCAATCACGCCCAGCGCACCGCCTAATGCTAATGAGCCTACATCGCCCATAAACACCTGCGCAGGATAAGTATTGAACCAAAGAAAACCCAAACCGGCACCCACAATGGCGGTACAGAACACCACTAATTCCGCACTGAATTTAATATACGGAATGTGTa > 1:835313/1‑251 (MQ=255) aGCCCACTTGTAAAATGACGGACAGGGTTTCCATGACAAACACGCCGCCCATAATTAATAATAAAAATTCCTGACGCACTAACACGGCAATCACGCCCAGCGCACCGCCTAATGCTAATGAGCCTACATCGCCCATAAACACCTGCGCAGGATAAGTATTGAACCAAAGAAAACCCAAACCGGCACCCACAATGGCGGTACAGAACACCACTAATTCCGCACTGAATTTAATATACGGAATGTGTAAata < 1:99960/250‑1 (MQ=255) gCCCACTTGTAAAATGACGGACAGGGTTTCCATGACAAACACGCCGCCCATAATTAATAATAAAAATTCCTGACGCACTAACACGGCAATCACGCCCAGCGCACCGCCTAATGCTAATGAGCCTACATCGCCCATAAACACCTGCGCAGGATAAGTATTGAACCAAAGAAAACCCAAACCGGCACCCACAATGGCGGTACAGAACACCACTAATTCCGCACTGAATTTAATATACGGAATGTGTAAata > 1:669054/1‑249 (MQ=255) cccACTTGTAAAATGACGGACAGGGTTTCCATGACAAACACGCCGCCCATAATTAATAATAAAAATTCCTGACGCACTAACACGGCAATCACGCCCAGCGCACCGCCTAATGCTAATGAGCCTACATCGCCCATAAACACCTGCGCAGGATAAGTATTGAACCAAAGAAAACCCAAACCGGCACCCACAATGGCGGTACAGAACACCACTAATTCCGCACTGAATTTAATATACGGAATGTGTAAatat < 1:504173/249‑1 (MQ=255) cccACTTGTAAAATGACGGACAGGGTTTCCATGACAAACACGCCGCCCATAATTAATAATAAAAATTCCTGACGCACTAACACGGCAATCACGCCCAGCGCACCGCCTAATGCTAATGAGCCTACATCGCCCATAAACACCTGCGCAGGATAAGTATTGAACCAAAGAAAACCCAAACCGGCACCCACAATGGCGGTACAGAACACCACTAATTCCGCACTGAATTTAATATACGGAATGTGTAAatat < 1:270621/249‑1 (MQ=255) cTTGTAAAATGACGGACAGGGTTTCCATGACAAACACGCCGCCCATAATTAATAATAAAAATTCCTGACGCACTAACACGGCAATCACGCCCAGCGCACCGCCTAATGCTAATGAGCCTACATCGCCCATAAACACCTGCGCAGGATAAGTATTGAACCAAAGAAAACCCAAACCGGCACCCACAATGGCGGTACAGAACACCACTAATTCCGCACTGAATTTAATATACGGAATGTGTAAATATTCGGCg > 1:87890/1‑251 (MQ=255) cTTGTAAAATGACGGACAGGGTTTCCATGACAAACACGCCGCCCATAATTAATAATAAAAATTCCTGACGCACTAACACGGCAATCACGCCCAGCGCACCGCCTAATGCTAATGAGCCTACATCGCCCATAAACACCTGCGCAGGATAAGCATTGAACCAAAGAAAACCCAAACCGGCACCCACAATGGCGGTACAGAACACCACTAATTCCGCACTGAATTTAATATACGGAATGTGTAAATATTCGGCg > 1:813770/1‑251 (MQ=255) aTGACGGACAGGGTTTCCATGACAAACACGCCGCCCATAATTAATAATAAAAATTCCTGACGCACTAACACGGCAATCACGCCCAGCGCACCGCCTAATGCTAATGAGCCTACATCGCCCATAAACACCTGCGCAGGATAAGTATTGAACCAAAGAAAACCCAAACCGGCACCCACAATGGCGGTACAGAac < 1:690201/192‑1 (MQ=255) gggTTTCCATGACAAACACGCCGCCCATAATTAATAATAAAAATTCCTGACGCACTAACACGGCAATCACGCCCAGCGCACCGCCTAATGCTAATGAGCCTACATCGCCCATAAACACCTGCGCAGGATAAGTATTGAACCAAAGAAAACCCAAACCGGCACCCACAATGGCGGTACAGAACACCACTAATTCCGCACTGAATTTAATATACGGAATGTGTAAata < 1:236682/226‑1 (MQ=255) gTTTCCATGACAAACACGCCGCCCATAATTAATAATAAAAATTCCTGACGCACTAACACGGCAATCACGCCCAGCGCACCGCCTAATGCTAATGAGCCTACATCGCCCATAAACACCTGCGCAGGATAAGTATTGAACCAAAGAAAACCCAAACCGGCACCCACAATGGCGGTACAGAACACCACTAATTCCGCACTGAATTTAATATACGGAATGTGTAAATATTCGGc > 1:8369/1‑230 (MQ=255) tGACAAACACGCCGCCCATAATTAATAATAAAAATTCCTGACGCACTAACACGGCAATCACGCCCAGCGCACCGCCTAATGCTAATGAGCCTACATCGCCCATAAACACCTGCGCAGGATAAGTATTGAACCAAAGAAAACCCAAACCGGCACCCACAATGGCGGTACAGAACACCACTAATTCCGCACTGAATTTAATATACGGAATGTGTAAATATTCGGCGAAATTAATGTTCCCCGTCGCCCATGc > 1:519659/1‑250 (MQ=255) cAAACACGCCGCCCATAATTAATAATAAAAATTCCTGACGCACTAACACGGCAATCACGCCCAGCGCACCGCCTAATGCTAATGAGCCTACATCGCCCATAAACACCTGCGCAGGATAAGTATTGAACCAAAGAAAACCCAAACCGGCACCCACAATGGCGGTACAGAACACCACTAATTCCGCACTGAATTTAATATACGGAATGTGTAAATATTCGGCGAAATTAATGTTCCCCGTCGCCCATGccacc < 1:177978/251‑1 (MQ=255) cacGCCGCCCATAATTAATAATAAAAATTCCTGACGCACTAACACGGCAATCACGCCCAGCGCACCGCCTAATGCTAATGAGCCTACATCGCCCATAAACACCTGCGCAGGATAAGCATTGAACCAAAGAAAACCCAAACCGGCACCCACAATTGCGGTACAGAACACCACTAATTCCGCAATGAATTTAATATACGGAATGTGTAAATATTCGGCGAAATTAATGTTCCCCGTCCCCCCTCCCACCAAgg > 1:347933/1‑251 (MQ=255) gccCATAATTAATAATAAAAATTCCTGACGCACTAACACGGCAATCACGCCCAGCGCACCGCCTAATGCTAATGAGCCTACATCGCCCATAAACACCTGCGCAGGATAAGTATTGAACCAAAGAAAACCCAAACCGGCACCCACAATGGCGGTACAGa > 1:67508/1‑158 (MQ=255) gccCATAATTAATAATAAAAATTCCTGACGCACTAACACGGCAATCACGCCCAGCGCACCGCCTAATGCTAATGAGCCTACATCGCCCATAAACACCTGCGCAGGATAAGTATTGAACCAAAGAAAACCCAAACCGGCACCCACAATGGCGGTACAGAACACCACTAATTCCGCACTGAATTTAATATACGGAATGTGTAAATATTCGGCGAAATTAATGTTCCCCGTCGCCCATGCCACCAAGGCAAATg > 1:38364/1‑251 (MQ=255) ccATAATTAATAATAAAAATTCCTGACGCACTAACACGGCAATCACGCCCAGCGCACCGCCTAATGCTAATGAGCCTACATCGCCCATAAACACCTGCGCAGGATAAGTATTGAACCAAAGAAAACCCAAACCGGCACCCACAATGGCGGTACAGAACACCACTAAtt > 1:70802/1‑168 (MQ=255) aataaAAATTCCTGACGCACTAACACGGCAATCACGCCCAGCGCACCGCCTAATGCTAATGAGCCTACATCGCCCATAAACACCTGCGCAGGATAAGTATTGAACCAAAGAAAACCCAAACCGGCACCCACAATGGCGGTACAGAACACCACTAATTCCGCACTGAATTTAATATACGGAATGTGTAAatat > 1:61798/1‑192 (MQ=255) taataaTTCCTGACTCACTAACACGGCAATCTCGCCCAGCGCACCGCCTAATGCTAATGAGGCTACATCGCCCATAAGCACCTGCGCAGGATAAGTATTGAACCAAAGAAAACCCAAACCAGCACCCACAATGGCGGGATAGAGCACCACTAATTCCGCACTGAATTTAATATACGGAATGTGTAAata < 1:164768/185‑1 (MQ=255) cGCACTAACACGGCAATCACGCCCAGCGCACCGCCTAATGCTAATGAGCCTACATCGCCCATAAACACCTGCGCAGGATAAGTATTGAACCAAAGAAAACCCAAACCGGCACCCACAATGGCGGTACAGAACACCACTAATTCCGCTCTGAATTTAATATACGGAATGTGTAAATATTCGGCGAAATTAATGTTCCCCGTCGCCCATGCCACCAAGGCAAATGCACCGGCAACAAGCACCGTCGGCATAAt > 1:596964/1‑251 (MQ=255) cACTAACACGGCAATCACGCCCAGCGCACCGCCTAATGCTAATGAGCCTACATCGCCCATAAACACCTGCGCAGGATAAGTATTGAACCAAAGAAAACCCAAACCGGCACCCACAATGGCGGTACAGAACACCACTAATTCCGCACTGAATTTAATATACGGAATGTGTaaa > 1:603616/1‑172 (MQ=255) cACTAACACGGCAATCACGCCCAGCGCACCGCCTAATGCTAATGAGCCTACATCGCCCATAAACACCTGCGCAGGATAAGTATTGAACCAAAGAAAACCCAAACCGGCACCCACAATGGCGGTACAGAACACCACTAATTCCGCACTGAATTTAATATACGGAATGTGTAAATATTCGGCGAAATTAATGTTCCCCGTCGCCCATGCCACCAAGGCAAATGCACCGGCAACAAGCACCGTCGGCATAATTg < 1:162943/251‑1 (MQ=255) cACTAACACGGCAATCACGCCCAGCGCACCGCCTAATGCTAATGAGCCTACATCGCCCATAAACACCTGCGCAGGATAAGTATTGAACCAAAGAAAACCCAAACCGGCACCCACAATGGCGGTACAGAACACCACTAATTCCGCACTGAATTTAATATACGGAATGTGTAAATATTCGGCGAAATTAATGTTCCCCGTCGCCCATGCCACCAAGGCAAATGCACCGGCAACAAGCACCGTCGGCATAATTg > 1:160582/1‑251 (MQ=255) cTAACACGGCAATCACGCCCAGCGCACCGCCTAATGCTAATGAGCCTACATCGCCCATAAACACCTGCGCAGGATAAGTATTGAACCAAAGAAAACCCAAACCGGCACCCACAATGGCGGTACAGAACACCACTAATTCCGCACTGAATTTAATATACGGAATGTGTAAATATTCGGCGAAATTAATGTTCCCCGTCGCCCATGCCACCAAGGCAAATGCACCGGCAACAAGCACCGTCGGCATAATTGc > 1:696143/1‑250 (MQ=255) aaCACGGCAATCACGCCCAGCGCACCGCCTAATGCTAATGAGCCTACATCGCCCATAAACACCTGCGCAGGATAAGTATTGAACCAAAGAAAACCCAAACCGGCACCCACAATGGCGGTACAGAACACCACTAATTCCGCACTGAATTTAATATACGGAATGTGTAAATATTCGGCGAAATTAATGTTCCCCGTCGCCCATGCCACCAAGGCAAATGCACCGGCAACAAGCACCGTCGGCATAATTGccaa > 1:508385/1‑251 (MQ=255) aaTCACGCCCAGCGCACCGCCTAATGCTAATGAGCCTACATCGCCCATAAACACCTGCGCAGGATAAGTATTGAACCAAAGAAAACCCAAACCGGCACCCACAATGGCGGTACAGAACACCACTAATTCCGCACTGAATTTAATATACGGAATGTGTAAATATTCGGCGAAATTAATGTTCCCCGTCGCCCATGCCACCAAGGCAAATGCACCGGCAACAAGCACCGTCGGCATAATTGccaagccgtcca > 1:206455/1‑251 (MQ=255) aTCACGCCCAGCGCACCGCCTAATGCTAATGAGCCTACATCGCCCATAAACACCTGCGCAGGATAAGTATTGAACCAAAGAAAACCCAAACCGGCACCCACAATGGCGGTACAGAACACCACTAATTCCGCACTGAATTTAATATACGGAATGTGTAAATATTCGGCGAAATTAATGTTCCCCGTCGCCCATGCCACCAAGGCAAATGCACCGGCAACAAGCACCGTCGGCATAATTGccaagccgtccaa > 1:793756/1‑251 (MQ=255) aCGCCCAGCGCACCGCCTAATGCTAATGAGCCTACATCGCCCATAAACACCTGCGCAGGATAAGTATTGAACCAAAGAAAACCCAAACCGGCACCCACAATGGCGGTACAGAACACCACTAATTCCGCACTGAATTTAATATACGGAATGTGTAAATATTCGGCGAAATTAATGTTCCCCGTCGCCCATGCCACCAAGGCAAATGCACCGGCAACAAGCACCGTCGGCATAATTGccaagccgtccaagcc < 1:695065/251‑1 (MQ=255) cccAGCGCACCGCCTAATGCTAATGAGCCTACATCGCCCATAAACACCTGCGCAGGATAAGTATTGAACCAAAGAAAACCCAAACCGGCACCCACAATGGCGGTACAGAACACCACt > 1:17661/1‑117 (MQ=255) cccAGCGCACCGCCTAATGCTAATGAGCCTACATCGCCCATAAACACCTGCGCAGGATAAGTATTGAACCAAAGAAAACCCAAACCGGCACCCACAATGGCGGTACAGAACACCACTAATTCCGCACTGAATTTAATATACGGAATGTGTAAATATTCGGCGAAATTAATGTTCCCCGTCGCCCATGCCACCAAGGCAAATg < 1:253615/202‑1 (MQ=255) gCCTAATGCTAATGAGCCTACATCGCCCATAAACACCTGCGCAGGATAAGTATTGAACCAAAGAAAACCCAAACCGGCACCCACAATGGCGGTACAGAACACCACTAATTCCGCACTGAATTTAATATACGGAATGTGTAAATATTCGGCGAAATTAATGTTCCCCGTCGCCCATGCCACCAAGGCAAATGCACCGGCAACAAGCACCGTCGGCATAATTGCCAAGCCGTCCAAGCCGTCGGTGAAATTCa > 1:684076/1‑251 (MQ=255) atgctaatgAGCCTACATCGCCCATAAACACCTGCGCAGGATAAGTATTGAACCAAAGAAAACCCAAACCGGCACCCACAATGGCGGTACAGAACACCACTAATTCCGCACTGAATTTAATATACGGAATGTGTAAATATTCGGCGAAATTAATGTTCCCCGTCGCCCATGCCACCAAGGCAAATGCACCGGCAACAAGCACCGTCGGCATAATTGccaagccgtccaagccg > 1:238619/1‑233 (MQ=255) tgctaatgAGCCTACATCGCCCATAAACACCTGCGCAGGATAAGTATTGAACCAAAGAAAACCCAAACCGGCACCCACAATGGCGGTACAGAACACCACTAATTCCGCACTGAATTTAATATACGGAATGTGTAAATATTCGGCGAAATTAATGTTCCCCGTCGCCCATGCCACCAAGGCAAATGCACCGGCAACAAGCACCGTCGGCATAATTGCCAAGCCGTCCAAGCCGTCGGTGAAATTCACCGCGt > 1:25602/1‑251 (MQ=255) tgAGCCTACATCGCCCATAAACACCTGCGCAGGATAAGTATTGAACCAAAGAAAACCCAAACCGGCACCCACAATGGCGGTACAGAACACCACTAATTCCGCACTGAATTTAATATACGGAATGTGTAAATATTCGGCGAAATTAATGTTCCCCGTCGCCCATGCCACCAAGGCAAATGCACCGGCAACAAGCACCGTCGGCATAATTGCCAAGCCGTCCAAGCCGTCGGTGAAATTCACCGCGTTACTgg < 1:454914/251‑1 (MQ=255) aGCCTACATCGCCCATAAACACCTGCGCAGGATAAGTATTGAACCAAAGAAAACCCAAACCGGCACCCACAATGGCGGTACAGAACACCACTAATTCCGCACTGAATTTAATATACGGAATGTGTAAATATTCGGCGAAATTAATGTTCCCCGTCGCCCATGCCACCAAGGCAAATGCACCGGCAACAAGCACCGTCGGCATAATTGCCAAGCCGTCCAAGCCGTCGGTGAAATTCACCGCGTTACTGGAg > 1:166928/1‑251 (MQ=255) aGCCTACATCGCCCATAAACACCTGCGCAGGATAAGTATTGAACCAAAGAAAACCCAAACCGGCACCCACAATGGCGGTACAGAACACCACTAATTCCGCACTGAATTTAATATACGGAATGTGTAAATATTCGGCGAAATTAATGTTCCCCGTCGCCCATGCCACCAAGGCAAATGCACCGGCAACAAGCACCGTCGGCATAATTGCCAAGCCGTCCAAGCCGTCGGTGAAATTCACCGCGTTACTGGAg > 1:464709/1‑251 (MQ=255) aGCCTACATCGCCCATAAACACCTGCGCAGGATAAGTATTGAACCAAAGAAAACCCAAACCGGCACCCACAATGGCGGTACAGAACACCACTAATTCCGCACTGAATTTAATATACGGAATGAGTAAATATTCGGCGAAATTAATGTTCCCCGTCGCCCATGCCACCAAGGCAAATGCACCGGCAACAAGCACCGTCGGCATAATTGCCAAGCCGTCCAAGCCGTCGGTGAAATTCACCGCGTTACTGGAg > 1:845385/1‑251 (MQ=255) ccTACATCGCCCATAAACACCTGCGCAGGATAAGTATTGAACCAAAGAAAACCCAAACCGGCACCCACAATGGCGGTACAGAACACCACTAATTCCGCACTGAATTTAATATACGGAATGTGTAAATATTCGGCGAAATTAATGTTCCCCGTCGCCCATGCCACCAAGGCAAATGCACCGGCAACAAGCACCGTCGGCATAATTGCCAAGCCGTCCAAGCCGTCGGTGAAATTCACCGCGTTACTGGAg < 1:607458/249‑1 (MQ=255) ccTACATCGCCCATAAACACCTGCGCAGGATAAGTATTGAACCAAAGAAAACCCAAACCGGCACCCACAATGGCGGTACAGAACACCACTAATTCCGCACTGAATTTAATATACGGAATGTGTAAATATTCGGCGAAATTAATGTTCCCCGTCGCCCATGCCACCAAGGCAAATGCACCGGCAACAAGCACCGTCGGCATAATTGCCAAGCCGTCCAAGCCGTCGGTGAAATTCACCGCGTTACTGGAGc > 1:381342/1‑250 (MQ=255) cccATAAACACCTGCGCAGGATAAGTATTGAACCAAAGAAAACCCAAACCGGCACCCACAATGGCGGTACAGAACACCACTAATTCCGCACTGAATTTAATATACGGaa > 1:179440/1‑109 (MQ=255) cccATAAACACCTGCGCAGGATAAGTATTGAACCAAAGAAAACCCAAACCGGCACCCACAATGGCGGTACAGAACACCACTAATTCCGCACTGAATTTAATATACGGAATGTGTAAATATTCGGCGAAATTAATGTTCCCCGTCGCCCATGCCACCAAGGCAAATGCACCGGCAACAAGCACCGTCGGCATAATTGCCAAGCCGTCCAAGCCGTCGGTGAAATTCACCGCGTTACTGGAGCCGACGATaa > 1:679418/1‑250 (MQ=255) cccATAAACACCTGCGCAGGATAAGTATTGAACCAAAGAAAACCCAAACCGGCACCCACAATGGCGGTACAGAACACCACTAATTCCGCACTGAATTTAATATACGGAATGTGTAAATATTCGGCGAAATTAATGTTCCCCGTCGCCCATGCCACCAAGGCAAATGCACCGGCAACAAGCACCGTCGGCATAATTGCCAAGCCGTCCAAGCCGTCGGTGAAATTCACCGCGTTACTGGAGCCGACGATaa > 1:716011/1‑250 (MQ=255) aTAAACACCTGCGCAGGATAAGTATTGAACCAAAGAAAACCCAAACCGGCACCCACAATGGCGGTACAGAACATCACTAATTCCGCACTGAATTTAATATACGGAATGTGTAAATATTCGGCGAAATTAATGTTCCCCGTCGCCCATGCCACCAAGGCAAATGCACCGGCAACAAGCACCGTCGGCATAATTGCCAAGCCGTCCAAGCCGTCGGTGAAATTCACCGCGTTACTGGAGCcgacg < 1:432189/243‑1 (MQ=255) aTAAACACCTGCGCAGGATAAGTATTGAACCAAAGAAAACCCAAACCGGCACCCACAATGGCGGTACAGAACACCACTAATTCCGCACTGAATTTAATATACGGAATGTGTAAATATTCGGCGAAATTAATGTTCCCCGTCGCCCATGCCACCAAGGCAAATGCACCGGCAACAAGCACCGTCGGCATAATTGCCAAGCCGTCCAAGCCGTCGGTGAAATTCACCGCGTTACTGGAGCCGACGATAACGaa > 1:124815/1‑251 (MQ=255) aTAAACACCTGCGCAGGATAAGTATTGAACCAAAGAAAACCCAAACCGGCACCCACAATGGCGGTACAGAACACCACTAATTCCGCACTGAATTTAATATACGGAATGTGTAAATATTCGGCGAAATTAATGTTCCCCGTCGCCCATGCCACCAAGGCAAATGCACCGGCAACAAGCACCGTCGGCATAATTGCCAAGCCGTCCAAGCCGTCGGTGAAATTCACCGCGTTACTGGAGCCGACGATAACGaa > 1:550017/1‑251 (MQ=255) ccTGCGCAGGATAAGTATTGAACCAAAGAAAACCCAAACCGGCACCCACAATGGCGGTACAGAACACCACTAATTCCGCACTGAATTTAATATACGGAATGTGTAAATATTCGGCGAAATTAATGTTCCCCGTCGCCCATGCCACCAAGGCAAATGCACCGGCAACAAGCACCGTCGGCATAATTGCCAAGCCGTCCAAGCCGTCGGTGAAATTCACCGCGTTACTGGAGCCGACGATAACGAAATAAGAc < 1:341991/251‑1 (MQ=255) gcgcAGGATAAGTATTGAACCAAAGAAAACCCAAACCGGCACCCACAATGGCGGTACAg > 1:263800/1‑59 (MQ=255) gcgcAGGATAAGTATTGAACCAAAGAAAACCCAAACCGGCACCCACAATGGCGGTACAGAACACCACTAATTCCGCACTGAATTTAATATACGGAATGTGTAAATATTCGGCGAAATTAATGTTCCCCGTCGCCCATGCCACCAAGGCAAATGCACCGGCAACAAGCACCGTCGGCATAATTGCCAAGCCGTCCAAGCCGTCGGTGAAATTAACCGCGTTACTGGAGCCGACGATAACGAAATAAGACAg > 1:62994/1‑250 (MQ=255) cAGGATAAGTATTGAACCAAAGAAAACCCAAACCGGCACCCACAATGGCGGTACAGAACACCACTAATTCCGCACTGAATTTAATATACGGAATGTGTAAATATTCGGCGAAATTAATGTTCCCCGTCGCCCATGCCACCAAGGCAAATGCACCGGCAACAAGCACCGTCGGCATAATTGCCAAGCCGTCCAAGCCGTCGGTGAAATTCACCGCGTTACTGGAGCCGACGATAACGAAATAAGACAGCAc < 1:339285/250‑1 (MQ=255) cAGGATAAGTATTGAACCAAAGAAAACCCAAACCGGCACCCACAATGGCGGTACAGAACACCACTAATTCCGCACTGAATTTAATATACGGAATGTGTAAATATTCGGCGAAATTAATGTTCCCCGTCGCCCATGCCACCAAGGCAAATGCACCGGCAACAAGCACCGTCGGCATAATTGCCAAGCCGTCCAAGCCGTCGGTGAAATTCACCGCGTTACTGGAGCCGACGATAACGAAATAAGACAGCACg > 1:458990/1‑251 (MQ=255) cAGGATAAGTATTGAACCAAAGAAAACCCAAACCGGCACCCACAATGGCGGTACAGAACACCACTAATTCCGCACTGAATTTAATATACGGAATGTGTAAATATTCGGCGAAATTAATGTTCCCCGTCGCCCATGCCACCAAGGCAAATGCACCGGCAACAAGCACCGTCGGCATAATTGCCAAGCCGTCCAAGCCGTCGGTGAAATTCACCGCGTTACTGGAGCCGACGATAACGAAATAAGACAGCACg > 1:293887/1‑251 (MQ=255) ggATAAGTATTGAACCAAAGAAAACCCAAACCGGCACCCACAATGGCGGTACAGAACACCACTAATTCCGCACTGAATTTAATATACGGAATGTGTAAATATTCGGCGAAATTAATGTTCCCCGTCGCCCATGCCACCAAGGCAAATGCACCGGCAACAAGCACCGTCGGCATAAt > 1:868010/1‑176 (MQ=255) ggATAAGTATTGAACCAAAGAAAACCCAAACCGGCACCCACAATGGCGGTACAGAACACCACTAATTCCGCACTGAATTTAATATACGGAATGTGTAAATATTCGGCGAAATTAATGTTCCCCGTCGCCCATGCCACCAAGGCAAATGCACCGGCAACAAGCACCGTCGGCATAATTGCCAAGCCGTCCAAGCCGTCGGTGAAATTCACCGCGTTACTGGAGCCGACGATAACGAAATAAGACAGCACGat > 1:638998/1‑251 (MQ=255) ggATAAGTATTGAACCAAAGAAAACCCAAACCGGCACCCACAATGGCGGTACAGAACACCACTAATTCCGCACTGAATTTAATATACGGAATGTGTAAATATTCGGCGAAATTAATGTTCCCCGTCGCCCATGCCACCAAGGCAAATGCACCGGCAACAAGCACCGTCGACATAATTGCCAAGCCGTCCAAGCCGTCGGTGAAATTCACCGCGTTACTGGAGCCGACGATAACGAAATAAGACAGCACGat > 1:430828/1‑251 (MQ=255) aTAAGTATTGAACCAAAGAAAACCCAAACCGGCACCCACAATGGCGGTACAGAACACCACTAATTCCGCACTGAATTTAATATACGGAATGTGTAAATATTCGGCGAAATTAATGTTCCCCGTCGCCCATGCCACCAAGGCAAATGCACCGGCAACAAGCACCGTCGGCATAATTGCCAAGCCGTCCAAGCCGTCGGTGAAATTCACCGCGTTACTGGAGCCGACGATAACGAAATAAGACAGCACGatat < 1:21921/251‑1 (MQ=255) gTATTGAACCAAAGAAAACCCAAACCGGCACCCACAATGGCGGTACAGAACACCACTAATTCCGCACTGAATTTAATATACGGAATGTGTAAATATTCGGCGAAATTAATGTTCCCCGTCGCCCATGCCACCAAGGCAAATGCACCGGCAACAAGCACCGTCGGCATAATTGCCAAGCCGTCCAAGCCGTCGGTGAGATTCACCGCGTTACTGGAGCCGACGATAACGAAATAAGACAGCACGATATaaaa > 1:679852/1‑251 (MQ=255) gTATTGAACCAAAGAAAACCCAAACCGGCACCCACAATGGCGGTACAGAACACCACTAATTCCGCACTGAATTTAATATACGGAATGTGTAAATATTCGGCGAAATTAATGTTCCCCGTCGCCCATGCCACCAAGGCAAATGCACCGGCAACAAGCACCGTCGGCATAATTGCCAAGCCGTCCAAGCCGTCGGTGAAATTCACCGCGTTACTGGAGCCGACGATAACGAAATAAGACAGCACGATATaaaa > 1:360304/1‑251 (MQ=255) tATTGAACCAAAGAAAACCCAAACCGGCACCCACAATGGCGGTACAGAACACCACTAATTCCGCACTGAATTTAATATACGGAATGTGTAAATATTCGGCGAAATTAATGTTCCCCGTCGCCCATGCCACCAAGGCAAATGCACCGGCAACAAGCACCGTCGGCATAATTGCCAAGCCGTCCAAGCCGTCGGTGAAATTCACCGCGTTACTGGAGCCGACGATAACGAAATAAGACAGCACGATATAAAAg < 1:808596/251‑1 (MQ=255) tATTGAACCAAAGAAAACCCAAACCGGCACCCACAATGGCGGTACAGAACACCACTAATTCCGCACTGAATTTAATATACGGAATGTGTAAATATTCGGCGAAATTAATGTTCCCCGTCGCCCATGCCACCAAGGCAAATGCACCGGCAACAAGCACCGTCGGCATAATTGCCAAGCCGTCCAAGCCGTCGGTGAAATTCACCGCGTTACTGGAGCCGACGATAACGAAATAAGACAGCACGATATAAAAg < 1:576987/251‑1 (MQ=255) aaaGAAAACCCAAACCGGCACCCACAATGGCGGTACAGAACACCACTAATTCCGCACTGAATTTAATATACGGAATGTGTAAATATTCGGCGAAATTAATGTTCCCCGTCGCCCATGCCACCAAGGCAAATGCACCGGCAACAAGCACCGTCGGCATAATTGCCAAGCCGTCCAAGCCGTCGGTGAAATTCACCGCGTTACTGGAGCCGACGATAACGAAATAAGACAGCACGATATAAAAGACACCGAGt > 1:267947/1‑251 (MQ=255) aaGAAAACCCAAACCGGCACCCACAATGGCGGTACAGAACACCACTAATTCCGCACTGAATTTAATATACGGAATGTGTAAATATTCGGCGAAATTAATGTTCCCCGTCGCCCATGCCACCAAGGCAAATGCACCGGCAACAAGCACCGTCGGCATAATTGCCAAGCCGTCCAAGCCGTCGGTGAAATTCACCGCGTTACTGGAGCCGACGATAACGAAATAAGACAGCACGATATAAAAGACACCGAGtt > 1:788481/1‑251 (MQ=255) aGAAAACCCAAACCGGCACCCACAATGGCGGTACAGAACACCACTAATTCCGCACTGAATTTAATATACGGAATGTGTa > 1:302386/1‑79 (MQ=255) aCCCAAACCGGCACCCACAATGGCGGTACAGAACACCACTAATTCCGCACTGAATTTAATATACGGAATGTGTAAATATTCGGCGAAATTAATGTTCCCCGTCGCCCATGCCACCAAGGCAAATGCACCGGCAACAAGCACCGTCGGCATAATTGCCAAGCCGTCCAAGCCGTCGGTGAAATTCACCGCGTTACTGGAGCCGACGATAACGAAATAAGACAGCACGATATAAAAGACACCGAGTTGTGGCa < 1:453488/251‑1 (MQ=255) | CGAAATGGTGATGAATCGGCGCCATCAGGAAAATACGTTGTTTATTACGCAGTTTGTAGGAGCCCACTTGTAAAATGACGGACAGGGTTTCCATGACAAACACGCCGCCCATAATTAATAATAAAAATTCCTGACGCACTAACACGGCAATCACGCCCAGCGCACCGCCTAATGCTAATGAGCCTACATCGCCCATAAACACCTGCGCAGGATAAGTATTGAACCAAAGAAAACCCAAACCTGCACCCACAATGGCGGTACAGAACACCACTAATTCCGCACTGAATTTAATATACGGAATGTGTAAATATTCGGCGAAATTAATGTTCCCCGTCGCCCATGCCACCAAGGCAAATGCACCGGCAACAAGCACCGTCGGCATAATTGCCAAGCCGTCCAAGCCGTCGGTGAAATTCACCGCGTTACTGGAGCCGACGATAACGAAATAAGACAGCACGATATAAAAGACACCGAGTTGTGGCA > 1/1456428‑1456910 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |