Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,444,202	G→A	N54N (AAC→AAT)	unknown ←	Chaperone protein HtpG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,444,202	0	G	A	100.0%	178.4 / NA	64	N54N (AAC→AAT)	unknown	Chaperone protein HtpG
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (20/44);  total (20/44)

ATAAGCTGGCTATCTTTCGCCTGGTCGCTGCCGAGAGCCGCTAAAAATTCTTTCGTGCCGGATTTGGCGATCGTACCCAAATGATCGATAACCTGCTCGCGGGTCATACCGATACCATTATCGCTTACGGTTAAGGTGCCTTTCTCGCTGTCAAAGCTGATACACACGCGCAATTCGCCGTCGCCTGCATATAAATCCGGGTTGGAAAGAGCTTTAAAACGCAATTTATCCGCCGCATCGGAGGCGTTGGAAATCAGCTCGCGCAAGAAAATTTCTTTATTGGAATATAAAGAATGGATCATTAATTGTAACAGTTGTTTGACTTCCGACTGGAAGCCGCGGGTTTCTTGATTTGTACTCATCTTCTTCCTACTTATGTTGATAAATTAAAAACGTTATGGATATAAGTGCGGAAGGCAAAATTTCAAGAGCTGTAGTA  >  1/1444002‑1444440                                                                                                                                                                                                         |                                                                                                                                                                                                                                               aTAAGCTGGCTATCTTTCGCCTGGTCGCTGCCGAGAGCCGCTAAAAATTCTTTCGTGCCGGATTTGGCGATCGTACCCAAATGATCGATAACCTGCTCGCGGGTCATACCGATACCATTATCGCTTACGGTTAAGGTGCCTTTCTCGCTGTCAAAGCTGATACACACGCGCAATTCGCCGTCGCCTGCATATAAATCCGGATTGGAAAGAGCTTTAAAACGCAATTTATCCGCCGCATCGGAGGCGTTGGa                                                                                                                                                                                              <  1:60654/251‑1 (MQ=255)   aaGCTGGCTATCTTTCGCCTGGTCGCTGCCGAGAGCCGCTAAAAATTCTTTCGTGCCGGATTTGGCGATCGTACCCAAATGATCGATAACCTGCTCGCGGGTCATACCGATACCATTATCGCTTACGGTTAAGGTGCCTTTCTCGCTGTCAAAGCTGATACACACGCGCAATTCGCCGTCGCCTGCATATAAATCCGGATTGGAAAGAGCTTTAAAACGCAATTTATCCGCCGCATCGGAGGCGTTGGaaa                                                                                                                                                                                            <  1:731283/251‑1 (MQ=255)      cTGGCTATCTTTCGCCTGGTCGCTGCCGAGAGCCGCTAAAAATTCTTTCGTGCCGGATTTGGCGATCGTACCCAAATGATCGATAACCTGCTCGCGGGTCATACCGATACCATTATCGCTTACGGTTAAGGTGCCTTTCTCGCTGTCAAAGCTGATACACACGCGCAATTCGCCGTCGCCTGCATATAAATCCGGATTGGAAAGAGCTTTAAAACGCAATTTATCCGCCGCATCGGAGGCGTTGGAAATc                                                                                                                                                                                          <  1:293863/250‑1 (MQ=255)      cTGGCTATCTTTCGCCTGGTCGCTGCCGAGAGCCGCTAAAAATTCTTTCGTGCCGGATTTGGCGATCGTACCCAAATGATCGATAACCTGCTCGCGGGTCATACCGATACCATTATCGCTTACGGTTAAGGTGCCTTTCTCGCTGTCAAAGCTGATACACACGCGCAATTCGCCGTCGCCTGCATATAAATCCGGATTGGAAAGAGCTTTAAAACGCAATTTATCCGCCGCATCGGAGGCGTTGGAAATc                                                                                                                                                                                          <  1:180114/250‑1 (MQ=255)         gCTATCTTTCGCCTGGTCGCTGCCGAGAGCCGCTAAAAATTCTTTCGTGCCGGATTTGGCGATCGTACCCAAATGATCGATAACCTGCTCGCGGGTCATACCGATACCATTATCGCTTACGGTTAAGGTGCCTTTCTCGCTGTCAAAGCTGATACACACGCGCAATTCGCCGTCGCCTGCATATAAATCCGGATTGGAAAGAGCTTTAAAACGCAATTTATCCGCCGCATCGGAGGCGTTGGAAATCAGc                                                                                                                                                                                       <  1:191912/250‑1 (MQ=255)             tCTTTCGCCTGGTCGCTGCCGAGAGCCGCTAAAAATTCTTTCGTGCCGGATTTGGCGATCGTACCCAAATGATCGATAACCTGCTCGCGGGTCATACCGATACCATTATCGCTTACGGTTAAGGTGCCTTTCTCGCTGTCAAAGCTGATACACACGCGCAATTCGCCGTCGCCTGCATATAAATCCGGATTGGAAAGAGCTTTAAAACGCAATTTATCCGCCGCATCGGAgg                                                                                                                                                                                                     <  1:600189/232‑1 (MQ=255)                              gCCGAGAGCCGCTAAAAATTCTTTCGTGCCGGATTTGGCGATCGTACCCAAATGATCGATAACCTGCTCGCGGGTCATACCGATACCATTATCGCTTACGGTTAAGGTGCCTTTCTCGCTGTCAAAGCTGATACACACGCGCAATTCGCCGTCGCCTGCATATAAATCCGGATTGGAAAGAGCTTTAAAACGCAATTTATCCGCCGCATCGGAgg                                                                                                                                                                                                     <  1:282744/215‑1 (MQ=255)                              gCCGAGAGCCGCTAAAAATTCTTTCGTGCCGGATTTGGCGATCGTACCCAAATGATCGATAACCTGCTCGCGGGTCATACCGATACCATTATCGCTTACGGTTAAGGTGCCTTTCTCGCTGTCAAAGCTGATACACACGCGCAATTCGCCGTCGCCTGCATATAAATCCGGATTGGAAAGAGCTTTAAAACGCAATTTATCCGCCGCATCGGAGGCGTTGGAAATCAGCTCGCGCAAGAAAATTTCTTTAt                                                                                                                                                                 >  1:87597/1‑251 (MQ=255)                                             aaaTTCTTTCGTGCCGGATTTGACGATCGTACCCAAATGATCGATAACCTGCTCGCGGGTCATACCGATACCATTATCGCTTACGGTTAAGGTGCCTTTCTCGCTGTCAAAGCTGATACACACGCGCAATTCGCCGTCGCCTGCATATAAATCCGGATTGGAAAGAGCTTTAAAACGCAATTTATCCGCCGCATCGGAGGCGTTGGAAATCAGCTCGCGCAAGAAAATTTCTTTATTGGAATATAAAGAAt                                                                                                                                                  <  1:639889/251‑1 (MQ=255)                                                      cGTGCCGGATTTGGCGATCGTACCCAAATGATCGATAACCTGCTCGCGGGTCATACCGATACCATTATCGCTTACGGTTAAGGTGCCTTTCTCGCTGTCAAAGCTGATACACACGCGCAATTCGCCGTCGCCTGCATATAAATCCGGATTGGAAAGAGCTTTAAAACGCAATTTATCCGCCGCATCGGAGGCGTTGGAAATCAGCTCGCGCAAGAAAATTTCTTTATTGGAATATAAAGAATGGATCATTa                                                                                                                                         <  1:468487/251‑1 (MQ=255)                                                           cGGATTTGGCGATCGTACCCAAATGATCGATAACCTGCTCGCGGGTCATACCGATACCATTATCGCTTACGGTTAAGGTGCCTTTCTCGCTGTCAAAGCTGATACACACGCGCAATTCGCCGTCGCCTGCATATAAATCCGGATTGGAAAGAGCTTTAAAACGCAATTTATCCGCCGCATCGGAGGCGTTGGAAATCAGCTCGCGCAAGAAAATTTCTTTATTGGAATATAAAGAATGGATCATTAATTGt                                                                                                                                    <  1:398271/251‑1 (MQ=255)                                                             gATTTGGCGATCGTACCCAAATGATCGATAACCTGCTCGCGGGTCATACCGATACCATTATCGCTTACGGTTAAGGTGCCTTTCTCGCTGTCAAAGCTGATACACACGCGCAATTCGCCGTCGCCTGCATATAAATCCGGATTGGAAAGAGCTTTAAAACGCAATTTATCCGCCGCATCGGAGGCGTTGGAAATCAGCTCGCGCAAGAAAATTTCTTTATTGGAATATAAAg                                                                                                                                                     <  1:543511/232‑1 (MQ=255)                                                                ttGGCGATCGTACCCAAATGATCGATAACCTGCTCGCGGGTCATACCGATACCATTATCGCTTACGGTTAAGGTGCCTTTCTCGCTGTCAAAGCTGATACACACGCGCAATTCGCCGTCGCCTGCATATAAATCCGGATTGGAAAGAGCTTTAAAACGCAATTTATCCGCCGCATCGGAGGCGTTGGAAATCAGCTCGCGCAAGAAAATTTCTTTATTGGAATATAAAGAATGGATCATTAATTGTAACAg                                                                                                                               <  1:530813/251‑1 (MQ=255)                                                                ttGGCGATCGTACCCAAATGATCGATAACCTGCTCGCGGGTCATACCGATACCATTATCGCTTACGGTTAAGGTGCCTTTCTCGCTGTCAAAGCTGATACACACGCGCAATTCGCCGTCGCCTGCATATAAATCCGGATTGGAAAGAGCTTTAAAACGCAATTTATCCGCCGCATCGGAGGCGTTGGAAATCAGCTCGCGCAAGAAAATTTCTTTATTGGAATATAAAGAATGGATCATTAATTGTAACAg                                                                                                                               <  1:256436/251‑1 (MQ=255)                                                                         gTACCCAAATGATCGATAACCTGCTCGCGGGTCATACCGATACCATTATCGCTTACGGTTAAGGTGCCTTTCTCGCTGTCAAAGCTGATACACACGCGCAATTCGCCGTCGCCTGCATATAAATCCGGATTGGAAAGAGCTTTAAAACGCAATTTATCCGCCGCATCGGAGGCGTTGGAAATCAGCTCGCGCAAGAAAATTTCTTTATTGGAATATAAAGAAt                                                                                                                                                  <  1:167065/223‑1 (MQ=255)                                                                         gTACCCAAATGATCGATAACCTGCTCGCGGGTCATACCGATACCATTATCGCTTACGGTTAAGGTGCCTTTCTCGCTGTCAAAGCTGATACACACGCGCAATTCGCCGTCGCCTGCATATAAATCCGGATTGGAAAGAGCTTTAAAACGCAATTTATCCGCCGCATCGGAGGCGTTGGAAATCAGCTCGCGCAAGAAAATTTCTTTATTGGAATATAAAGAATGGATCATTAATTg                                                                                                                                     <  1:569660/236‑1 (MQ=255)                                                                         gTACCCAAATGATCGATAACCTGCTCGCGGGTCATACCGATACCATTATCGCTTACGGTTAAGGTGCCTTTCTCGCTGTCAAAGCTGATACACACGCGCAATTCGCCGTCGCCTGCATATAAATCCGGATTGGAAAGAGCTTTAAAACGCAATTTATCCGCCGCATCGGAGGCGTTGGAAATCAGCTCGCGCAAGAAAATTTCTTTATTGGAATATAAAGAATGGATCATTAATTGTAACAGTTGTTTGa                                                                                                                       >  1:630873/1‑250 (MQ=255)                                                                          tACCCAAATGATCGATAACCTGCTCGCGGGTCATACCGATACCATTATCGCTTACGGTTAAGGTGCCTTTCTCGCTGTCAAAGCTGATACACACGCGCAATTCGCCGTCGCCTGCATATAAATCCGGATTGGAAAGAGCTTTAAAACGCAATTTATCCGCCGCATCGGAGGCGTTGGAAATCAGCTCGCGCAAGAAAATTTCTTTATTGGAATATAAAGAATGGATCATTAATTGTAACAGTTGTTTGa                                                                                                                       >  1:255018/1‑249 (MQ=255)                                                                            cccAAATGATCGATAACCTGCTCGCGGGTCATACCGATACCATTATCGCTTACGGTTAAGGTGCCTTTCTCGCTGTCAAAGCTGATACACACGCGCAATTCGCCGTCGCCTGCATATAAATCCGGATTGGAAAGAGCTTTAAAACGCAATTTATCCGCCGCATCGGAGGCGTTGGaaa                                                                                                                                                                                            <  1:319758/178‑1 (MQ=255)                                                                                    aTCGATAACCTGCTCGCGGGTCATACCGATACCATTATCGCTTACGGTTAAGGTGCCTTTCTCGCTGTCAAAGCTGATACACACGCGCAATTCGCCGTCGCCTGCATATAAATCCGGATTGGAAAGAGCTTTAAAACGCAATTTATCCGCCGCATCGGAGGCGTTGGAAATCAGCTCGCGCAAGAAAATTTCTTTATTGGAATATAAAGAATGGATCATTAATTGTAACAGTTGTTTGACTTCCGACTGGa                                                                                                           >  1:354853/1‑251 (MQ=255)                                                                                     tCGATAACCTGCTCGCGGGTCATACCGATACCATTATCGCTTACGGTTAAGGTGCCTTTCTCGCTGTCAAAGCTGATACACACGCGCAATTCGCCGTCGCCTGCATATAAATCCGGATTGGAAAGAGCTTTAAAACGCAATTTATCCGCCGCATCGGAGGCGTTGGAAATCAGCTCGCGCAAGAAAATTTc                                                                                                                                                                      <  1:702446/191‑1 (MQ=255)                                                                                     tCGATAACCTGCTCGCGGGTCATACCGATACCATTATCGCTTACGGTTAAGGTGCCTTTCTCGCTGTCAAAGCTGATACACACGCGCAATTCGCCGTCGCCTGCATATAAATCCGGATTGGAAAGAGCTTTAAAACGCAATTTATCCGCCGCATCGGAGGCGTTGGAAATCAGCTCGCGCAAGAAAATTTCTTTATTGGAATATaa                                                                                                                                                       <  1:75810/206‑1 (MQ=255)                                                                                     tCGATAACCTGCTCGCGGGTCATACCGATACCATTATCGCTTACGGTTAAGGTGCCTTTCTCGCTGTCAAAGCTGATACACACGCGCAATTCGCCGTCGCCTGCATATAAATCCGGATTGGAAAGAGCTTTAAAACGCAATTTATCCGCCGCATCGGAGGCGTTGGAAATCAGCTCGCGCAAGAAAATTTCTTTATTGGAATATAAAGAATGGATCATTAATTGTAACAGTTGTTTGACTTCCGACTGGaa                                                                                                          >  1:582065/1‑251 (MQ=255)                                                                                               gCTCGCGGGTCATACCGATACCATTATCGCTTACGGTTAAGGTGCCTTTCTCGCTGTCAAAGCTGATACACACGCGCAATTCGCCGTCGCCTGCATATAAATCCGGATTGGAAAGAGCTTTAAAACGCAATTTATCCGCCGCATCGGAGGCGTTGGAAATCAGCTCGCGCAAGAAAATTTCTTTATTGGAATATAAAGAATGGATCATTAATTGTAACAGTTGTTTGACTTCCGACTGGAAGCCGCGGGtt                                                                                                >  1:171376/1‑251 (MQ=255)                                                                                                      ggTCATACCGATACCATTATCGCTTACGGTTAAGGTGCCTTTCTCGCTGTCAAAGCTGATACACACGCGCAATTCGCCGTCGCCTGCATATAAATCCGGATTGGAAAGAGCTTTAAAACGCAATTTATCCGCCGCATCGGAGGCGTTGGAAATc                                                                                                                                                                                          >  1:454383/1‑154 (MQ=255)                                                                                                      ggTCATACCGATACCATTATCGCTTACGGTTAAGGTGCCTTTCTCGCTGTCAAAGCTGATACACACGCGCAATTCGCCGTCGCCTGCATATAAATCCGGATTGGAAAGAGCTTTAAAACGCAATTTATCCGCCGCATCGGAGGCGTTGGAAATCAGCTCGCGCAAGAAAATTTCTTTATTGGAATATAAAGAATGGATc                                                                                                                                             >  1:746549/1‑199 (MQ=255)                                                                                                      ggTCATACCGATACCATTATCGCTTACGGTTAAGGTGCCTTTCTCGCTGTCAAAGCTGATACACACGCGCAATTCGCCGTCGCCTGCATATAAATCCGGATTGGAAAGAGCTTTAAAACGCAATTTATCCGCCGCATCGGAGGCGTTGGAAATCAGCTCGCGCAAGAAAATTTCTTTATTGGAATATAAAGAATGGATc                                                                                                                                             <  1:695483/199‑1 (MQ=255)                                                                                                      ggTCATACCGATACCATTATCGCTTACGGTTAAGGTGCCTTTCTCGCTGTCAAAGCTGATACACACGCGCAATTCGCCGTCGCCTGCATATAAATCCGGATTGGAAAGAGCTTTAAAACGCAATTTATCCGCCGCATCGGAGGCGTTGGAAATCAGCTCGCGCAAGAAAATTTCTTTATTGGAATATAAAGAATGGATCATTAATTGTAACAGTTGTTTGACTTCCGACTGGAAGCCGCGGGTTTCTTGAt                                                                                         >  1:361525/1‑251 (MQ=255)                                                                                                            aCCGATACCATTATCGCTTACGGTTAAGGTGCCTTTCTCGCTGTCAAAGCTGATACACACGCGCAATTCGCCGTCGCCTGCATATAAATCCGGATTGGAAAGAGCTTTAAAACGCAATTTATCCGCCGCATCGGAGGCGTTGGAAATCAGCTCGCGCAAGAAAATTTCTTTATTGGAATATAAAGAATGGATCATTAATTGTAACAGTTGTTTGACTTCCGACTGGAAGCCGCGGGTTTCTTGATTTGTa                                                                                    >  1:857553/1‑250 (MQ=255)                                                                                                            aCCGATACCATTATCGCTTACGGTTAAGGTGCCTTTCTCGCTGTCAAAGCTGATACACACGCGCAATTCGCCGTCGCCTGCATATAAATCCGGATTGGAAAGAGCTTTAAAACGCAATTTATCCGCCGCATCGGAGGCGTTGGAAATCAGCTCGCGCAAGAAAATTTCTTTATTGGAATATAAAGAATGGATCATTAATTGTAACAGTTGTTTGACTTCCGACTGGAAGCCGCGGGTTTCTTGATTTGTa                                                                                    >  1:278818/1‑250 (MQ=255)                                                                                                            aCCGATACCATTATCGCTTACGGTTAAGGTGCCTTTCTCGCTGTCAAAGCTGATACACACGCGCAATTCGCCGTCGCCTGCATATAAATCCGGATTGGAAAGAGCTTTAAAACGCAATTTATCCGCCGCATCGGAGGCGTTGGAAATCAGCTCGCGCAAGAAAATTTCTTTATTGGAATATAAAGAATGGATCATTAATTGTAACAGTTGTTTGACTTCCGACTGGAAGCCGCGGGTTTCTTGATTTGTAc                                                                                   <  1:147228/251‑1 (MQ=255)                                                                                                             ccGATACCATTATCGCTTACGGTTAAGGTGCCTTTCTCGCTGTCAAAGCTGATACACACGCGCAATTCGCCGTCGCCTGCATATAAATCCGGATTGGAAAGAGCTTTAAAACGCAATTTATCCGCCGCATCGGAGGCGTTGGAAATCAGCTCGCGCAAGAAAATTTCTTTATTGGAATATAAAGAATGGATCATTAATTGTAACAGTTGTTTGACTTCCGACTGGAAGCCGCGGGTTTCTTGATTTGTa                                                                                    >  1:641138/1‑249 (MQ=255)                                                                                                             ccGATACCATTATCGCTCACGGTTAAGGTGCCTTTCTCGCTGTCAAAGCTGATACACACGCGCAATTCGCCGTCGCCTGCATATAAATCCGGATTGGAAAGAGCTTTAAAACGCAATTTATCCGCCGCATCGGAGGCGTTGGAAATCAGCTCGCGCAAGAAAATTTCTTTATTGGAATATAAAGAAt                                                                                                                                                  <  1:454030/187‑1 (MQ=255)                                                                                                              cGATACCATTATCGCTTACGGTTAAGGTGCCTTTCTCGCTGTCAAAGCTGATACACACGCGCAATTCGCCGTCGCCTGCATATAAATCCGGATTGGAAAGAGCTTTAAAACGCAATTTATCCGCCGCATCGGAGGCGTTGGAAATCAGCTCGCGCAAGAAAATTTCTTTATTGGAATATaa                                                                                                                                                       >  1:720224/1‑181 (MQ=255)                                                                                                                 tACCATTATCGCTTACGGTTAAGGTGCCTTTCTCGCTGTCAAAGCTGATACACACGCGCAATTCGCCGTCGCCTGCATATAAATCCGGATTGGAAAGAGCTTTAAAACGCAATTTATCCGCCGCATCGGAGGCGTTGGAAATCAGCTCGCGCAAGAAAATTTCTTTATTGGAATATAAAGAATGGATCATTAATTGTAACAGTTGTTTGACTTCCGACTGGAAGCCGCGGGTTTCTTGATTTGTACTCAtc                                                                              <  1:75564/251‑1 (MQ=255)                                                                                                                 tACCATTATCGCTTACGGTTAAGGTGCCTTTCTCGCTGTCAAAGCTGATACACACGCGCAATTCGCCGTCGCCTGCATATAAATCCGGATTGGAAAAAACTTTAAAACGCAATTTATCCGCCGCATCGGAGGCGTTGGAAATCAGCTCGCGCAAGAAAATTTCTTTATTGGAATATAAAGAATGGATCATTAATTGTAACAGTTGTTTGACTTCCGACTGGAAGCCGCGGGTTTCTTGATTTGTACTCAtc                                                                              <  1:399680/251‑1 (MQ=255)                                                                                                                  aCCATTATCGCTTACGGTTAAGGTGCCTTTCTCGCTGTCAAAGCTGATACACACGCGCAATTCGCCGTCGCCTGCATATAAATCCGGATTGGAAAGAGCTTTAAAACGCAATTTATCCGCCGCATCGGAGGCGTTGGAAATCAGCTCGCGCAAGAAAATTTc                                                                                                                                                                      <  1:23625/162‑1 (MQ=255)                                                                                                                   ccATTATCGCTTACGGTTAAGGTGCCTTTCTCGCTGTCAAAGCTGATACACACGCGCAATTCGCCGTCGCCTGCATATAAATCCGGATTGGAAAGAGCTTTAAAACGCAATTTATCCGCCGCATCGGAGGCGTTGGAAATCAGCTCGCGCAAGAAAATTTCTTTATTGGAATATAAAGAATGGATCATTAATTg                                                                                                                                     <  1:464686/194‑1 (MQ=255)                                                                                                                   ccATTATCGCTTACGGTTAAGGTGCCTTTCTCGCTGTCAAAGCTGATACACACGCGCAATTCGCCGTCGCCTGCATATAAATCCGGATTGGAAAGAGCTTTAAAACGCAATTTATCCGCCGCATCGGAGGCGTTGGAAATCAGCTCGCGCAAGAAAATTTCTTTATTGGAATATAAAGAATGGATCATTAATTGTAACAGTTGTTTGACTTCCGACTGGAAGCCGCGGGTTTCTTGATTTGTACTCAtctt                                                                            >  1:87625/1‑251 (MQ=255)                                                                                                                    cATTATCGCTTACGGTTAAGGTGCCTTTCTCGCTGTCAAAGCTGATACACACGCGCAATTCGCCGTCGCCTGCATATAAATCCGGATTGGAAAGAGCTTTAAAACGCAATTTATCCGCCGCATCGGAGGCGTTGGAAATCAGCTCGCGCAAGAAAATTTCTTTATTGGAATATAAAGAATGGATCATTAATTGTAACAGTTGTTTGACTTCCGACTGGAAGCCGCGGGTTTCTTGATTTGTACTCAtcttc                                                                           <  1:180695/251‑1 (MQ=255)                                                                                                                        aTCGCTTACGGTTAAGGTGCCTTTCTCGCTGTCAAAGCTGATACACACGCGCAATTCGCCGTCGCCTGCATATAAATCCGGATTGGAAAGAGCTTTAAAACGCAATTTATCCGCCGCATCGGAGGCGTTGGAAATCAGCTCGCGCAAGAAAATTTCTTTATTGGAATATAAAGAATGGATCATTAATTGTAACAGTTGTTTGACTTCCGACTGGAAGCCGCggg                                                                                                  <  1:159498/224‑1 (MQ=255)                                                                                                                        aTCGCTTACGGTTAAGGTGCCTTTCTCGCTGTCAAAGCTGATACACACGCGCAATTCGCCGTCGCCTGCATATAAATCCGGATTGGAAAGAGCTTTAAAACGCAATTTATCCGCCGCATCGGAGGCGTTGGAAATCAGCTCGCGCAAGAAAATTTCTTTATTGGAATATAAAGAATGGATCATTAATTGTAACAGTTGTTTGACTTCCGACTGGAAGCCGCAGGTTTCTTGATTTGTACTCATCTTCTTcc                                                                       <  1:467357/251‑1 (MQ=255)                                                                                                                                cGGTTAAGGTGCCTTTCTCGCTGTCAAAGCTGATACACACGCGCAATTCGCCGTCGCCTGCATATAAATCCGGATTGGAAAGAGCTTTAAAACGCAATTTATCCGCCGCATCGGAGGCGTTGGAAATCAGCTCGCGCAAGAAAATTTc                                                                                                                                                                      >  1:494261/1‑148 (MQ=255)                                                                                                                                cGGTTAAGGTGCCTTTCTCGCTGTCAAAGCTGATACACACGCGCAATTCGCCGTCGCCTGCATATAAATCCGGATTGGAAAGAGCTTTAAAACGCAATTTATCCGCCGCATCGGAGGCGTTGGAAATCAGCTCGCGCAAGAAAATTTCTTTATTGGAATATAAAGAATGGATCATTAATTGTAACAGTTGTTTGACTTCCGACTGGAAGCCGCGGGTTTCTTGATTTGTACTCATCTTCTTCCTACTTATg                                                               >  1:269591/1‑251 (MQ=255)                                                                                                                                  gTTAAGGTGCCTTTCTCGCTGTCAAAGCTGATACACACGCGCAATTCGCCGTCGCCTGCATATAAATCCGGATTGGAAAGAGCTTTAAAACGCAATTTATCCGCCGCATCGGAGGCGTTGGAAATCAGCTCGCGCAAGAAAATTTCTTTATTGGAATATAAAGAATGGATCATTAATTGTAACAGTTGTTTGACTTCCGACTGGAAGCCGCGGGTTTCTTGATTTGTACTCATCTTCTTCCTACTTATGt                                                              <  1:277587/250‑1 (MQ=255)                                                                                                                                                      gTCAAAGCTGATACACACGCGCAATTCGCCGTCGCCTGCATATAAATCCGGATTGGAAAGAGCTTTAAAACGCAATTTATCCGCCGCATCGGAGGCGTTGGAAATCAGCTCGCGCAAGAAAATTTCTTTATTGGAATATAAAGAATGGATCATTAATTGTAACAGTTGTTTGACTTCCGACTGGAAGCCGCGGGTTTCTTGATTTGTACTCATCTTCTTCCTACTTATg                                                               <  1:780599/229‑1 (MQ=255)                                                                                                                                                             cTGATACACACGCGCAATTCGCCGTCGCCTGCATATAAATCCGGATTGGAAAGAGCTTTAAAACGCAATTTATCCGCCGCATCGGAGGCGTTGGAAATCAGCTCGCGCAAGAAAATTTCTTTATTGGAATATAAAGAATGGATCATTAATTGTAACAGTTGTTTGACTTCCGACTGGAAGCCGCGGGTTTCTTGATTTGTACTCATCTTCTTCCTACTTATGTTGATAAATTAAAAACGTTATGGATATaa                                  >  1:855859/1‑251 (MQ=255)                                                                                                                                                              tGATACACACGCGCAATTCGCCGTCGCCTGCATATAAATCCGGATTGGAAAGAGCTTTAAAACGCAATTTATCCGCCGCATTGGAGGCGTTGGAAATCAGCTCGCGCAAGAAAATTTCTTTATTGGAATATAAAGAATGGATCATTAATTGTAACAGTTGTTTGACTTCCGACTGGAAGCCGCGGGTTTCTTGATTTGTACTCATCTTCTTCCTACTTATGTTGATAAATTAAAAACGTTATGGATATAAg                                 <  1:448905/251‑1 (MQ=255)                                                                                                                                                              tGATACACACGCGCAATTCGCCGTCGCCTGCATATAAATCCGGATTGGAAAGAGCTTTAAAACGCAATTTATCCGCCGCATCGGAGGCGTTGGAAATCAGCTCGCGCAAGAAAATTTCTTTATTGGAATATAAAGAATGGATCATTAATTGTAACAGTTGTTTGACTTCCGACTGGAAGCCGCGGGTTTCTTGATTTGTACTCATCTTCTTCCTACTTATGTTGATAAATTAAAAACGTTATGGATATAAg                                 <  1:747469/251‑1 (MQ=255)                                                                                                                                                              tGATACACACGCGCAATTCGCCGTCGCCTGCATATAAATCCGGATTGGAAAGAGCTTTAAAACGCAATTTATCCGCCGCATCGGAGGCGTTGGAAATCAGCTCGCGCAAGAAAATTTCTTTATTGGAATATAAAGAATGGATCATTAATTGTAACAGTTGTTTGACTTCCGACTGGAAGCCGCGGGTTTCTTGATTTGTACTCATCTTCTTCCTACTTATGTTGATAAATTAAAAACGTTATGGATATAAg                                 <  1:324130/251‑1 (MQ=255)                                                                                                                                                              tGATACACACGCGCAATTCGCCGTCGCCTGCATATAAATCCGGATTGGAAAGAGCTTTAAAACGCAATTTATCCGCCGCATCGGAGGCGTTGGAAATCAGCTCGCGCAAGAAAATTTCTTTATTGGAATATAAAGAATGGATCATTAATTGTAACAGTTGTTTGACTTCCGACTGGAAGCCGCGGGTTTCTTGATTTGTACTCATCTTCTTCCTACTTATGTTGATAAATTAAAAACGTTATGGATATAAg                                 <  1:762786/251‑1 (MQ=255)                                                                                                                                                              tGATACACACGCGCAATTCGCCGTCGCCTGCATATAAATCCGGATTGGAAAGAGCTTTAAAACGCAATTTATCCGCCGCATCGGAGGCGTTGGAAATCAGCTCGCGCAAGAAAATTTCTTTATTGGAATATAAAGAATGGATCATTAATTGTAACAGTTGTTTGACTTCCGACTGGAAGCCGCGGGTTTCTTGATTTGTACTCATCTTCTTCCTACTTATGTTGATAAATTAAAAACGTTATGGATATAAg                                 <  1:533062/251‑1 (MQ=255)                                                                                                                                                               gATACACACGCGCAATTCGCCGTCGCCTGCATATAAATCCGGATTGGAAAGAGCTTTAAAACGCAATTTATCCGCCGCATCGGAGGCGTTGGAAATCAGCTCGCGCAAGAAAATTTc                                                                                                                                                                      <  1:111851/117‑1 (MQ=255)                                                                                                                                                                aTACACACGCGCAATTCGCCGTCGCCTGCATATAAATCCGGATTGGAAAGAGCTTTAAAACGCAATTTATCCGCCGCATCGGAGGCGTTGGAAATCAGCTCGCGCAAGAAAATTTCTTTATTGGAATATAAAGaa                                                                                                                                                   <  1:122885/135‑1 (MQ=255)                                                                                                                                                                      acGCGCAATTCGCCGTCGCCTGCATATAAATCCGGATTGGAAAGAGCTTTAAAACGCAATTTATCCGCCGCATCGGAGGCGTTGGAAATCAGCTCGCGCAAGAAAATTTCTTTATTGGAATATAAAGAATGGATCATTAATTGTAACAGTTGTTTGACTTCCGACTGGAAGCCGCGGGTTTCTTGATTTGTACTCATCTTCTTCCTACTTATGTTGATAAATTAAAAACGTTATGGATATAAGTGCGGAAg                         <  1:544397/251‑1 (MQ=255)                                                                                                                                                                      acGCGCAATTCGCCGTCGCCTGCATATAAATCCGGATTGGAAAGAGCTTTAAAACGCAATTTATCCGCCGCATCGGAGGCGTTGGAAATCAGCTCGCGCAAGAAAATTTCTTTATTGGAATATAAAGAATGGATCATTAATTGTAACAGTTGTTTGACTTCCGACTGGAAGCCGCGGGTTTCTTGATTTGTACTCATCTTCTTCCTACTTATGTTGATAAATTAAAAACGTTATGGATATAAGTGCGGAAg                         <  1:265153/251‑1 (MQ=255)                                                                                                                                                                                           gCATATAAATCCGGATTGGAAAGAGCTTTAAAACGCAATTTATCCGCCGCATCGGAGGCGTTGGAAATCAGCTCGCGCAAGAAAATTTCTTTATTGGAATATAAAGAATGGATCATTAATTGTAACAGTTGTTTGACTTCCGACTGGAAGCCGCGGGTTTCTTGATTTGTAc                                                                                   <  1:7544/172‑1 (MQ=255)                                                                                                                                                                                           gCATATAAATCCGGATTGGAAAGAGCTTTAAAACGCAATTTATCCGCCGCATCGGAGGCGTTGGAAATCAGCTCGCGCAAGAAAATTTCTTTATTGGAATATAAAGAATGGATCATTAATTGTAACAGTTGTTTGACTTCCGACTGGAAGCCGCGGGTTTCTTGATTTGTAc                                                                                   <  1:703046/172‑1 (MQ=255)                                                                                                                                                                                           gCATATAAATCCGGATTGGAAAGAGCTTTAAAACGCAATTTATCCGCCGCATCGGAGGCGTTGGAAATCAGCTCGCGCAAGAAAATTTCTTTATTGGAATATAAAGAATGGATCATTAATTGTAACAGTTGTTTGACTTCCGACTGGAAGCCGCGGGTTTCTTGATTTGTACTCATCTTCTTCCTACTTATGTTGATAAATTAAAAACGTTATGGATATAAg                                 >  1:152475/1‑222 (MQ=255)                                                                                                                                                                                            cATATAAATCCGGATTGGAAAGAGCTTTAAAACGCAATTTATCCGCCGCATCGGAGGCGTTGGAAATCAGCTCGCGCAAGAAAATTTCTTTATTGGAATATAAAGAATGGATCATTAATTGTAACAGTTGTTTGACTTCCGACTGGAAGCCGCGGGTTTCTTGATTTGTACTCATCTTCTTCCTACTTATGTTGATAAATTAAAAACGTTATGGATATAAGTGCGGAAGGCAAAATTTCAAGAGCTgtag    <  1:401317/250‑1 (MQ=255)                                                                                                                                                                                             atatAAATCCGGATTGGAAAGAGCTTTAAAACGCAATTTATCCGCCGCATCGGAGGCGTTGGAAATCAGCTCGCGCAAGAAAATTTCTTTATTGGAATATAAAg                                                                                                                                                     <  1:715334/104‑1 (MQ=255)                                                                                                                                                                                             atatAAATCCGGATTGGAAAGAGCTTTAAAACGCAATTTATCCGCCGCATCGGAGGCGTTGGAAATCAGCTCGCGCAAGAAAATTTCTTTATTGGAATATAAAGAATGGATCATTAATTGTAACAGTTGTTTGACTTCCGACTGGAAGCCGCGGGTTTCTTGATTTGTACTCATCTTCTTCCTACTTa                                                                 <  1:537270/188‑1 (MQ=255)                                                                                                                                                                                             atatAAATCCGGATTGGAAAGAGCTTTAAAACGCAATTTATCCGCCGCATCGGAGGCGTTGGAAATCAGCTCGCGCAAGAAAATTTCTTTATTGGAATATAAAGAATGGATCATTAATTGTAACAGTTGTTTGACTTCCGACTGGAAGCCGCGGGTTTCTTGATTTGTACTCATCTTCTTCCTACTTATGTTGATAAATTAAAAACGTTATGGATATAAGTGCGGAAGGCAAAATTTCAAGAGCTgtagta  >  1:471115/1‑251 (MQ=255)                                                                                                                                                                                              tataAATCCGGATTGGAAAGAGCTTTAAAACGCAATTTATCCGCCGCATCGGAGGCGTTGGAAATCAGCTCGCGCAAGAAAATTTCTTTATTGGAATATaaa                                                                                                                                                      >  1:550440/1‑102 (MQ=255)                                                                                                                                                                                                         |                                                                                                                                                                                                                                               ATAAGCTGGCTATCTTTCGCCTGGTCGCTGCCGAGAGCCGCTAAAAATTCTTTCGTGCCGGATTTGGCGATCGTACCCAAATGATCGATAACCTGCTCGCGGGTCATACCGATACCATTATCGCTTACGGTTAAGGTGCCTTTCTCGCTGTCAAAGCTGATACACACGCGCAATTCGCCGTCGCCTGCATATAAATCCGGGTTGGAAAGAGCTTTAAAACGCAATTTATCCGCCGCATCGGAGGCGTTGGAAATCAGCTCGCGCAAGAAAATTTCTTTATTGGAATATAAAGAATGGATCATTAATTGTAACAGTTGTTTGACTTCCGACTGGAAGCCGCGGGTTTCTTGATTTGTACTCATCTTCTTCCTACTTATGTTGATAAATTAAAAACGTTATGGATATAAGTGCGGAAGGCAAAATTTCAAGAGCTGTAGTA  >  1/1444002‑1444440

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑