Predicted mutation
evidence seq id position mutation annotation gene description
RA 1 1,435,561 C→T S230S (TCG→TCA)  unknown ← 7‑cyano‑7‑deazaguanine synthase (EC 6.3.4.20)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*11,435,5610CT100.0% 175.2 / NA 64S230S (TCG→TCA) unknown7‑cyano‑7‑deazaguanine synthase (EC 6.3.4.20)
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (35/29);  total (35/29)

TATGCTTTAACAACGTCGATTTACCGACCTGTCTCGCACCGGTGACAAGAACTGCCGGAAATTGCTTAAGTAGACGACTTACTAAAGGTTGGATTTGGCGGGAAAGGTACATATTCACTCTCAAAATTAGTTTAACTCAATACAGCATATTAAATTAAACTAATTTAAACAAGGGAAACTGTGAATTTTTAAGTTTAATTTCACATGACATATTGAATTGAACTAATTTTTAAATGTTACTCCCTCGAAAGTGCGGTTGTTTTCTCAGGCATTTTTTCCTCACGTTCCGGCAAATACGCCTTCAATCCACGTTCGCGCAAAACGCAACTCGGGCATTCGCCGCAACCGCCTTCGACACCGTTGTAGCAAGTGTGGGTGTGCCGGCGGATATAATCAAACGCGCCAAGTTGATCCGCCAGCGCCCAGGTTTCTTTTTTATCCAGCCACATTAACGGTGTGCGGATTTCAAAAGGGTAAGCCATGGCAAGGTT  >  1/1435316‑1435806
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                     
tatGCTTTAACAACGTCGATTTACCGACCTGTCTCGCACCGGTGACAAGAACTGCCGGAAATTGCTTAAGTAGACGACTTACTAAAGGTTGGATTTGGCGGGAAAGGTACATATTCACTCTCAAAATTAGTTTAACTCAATACAGCATATTAAATTAAACTAATTTAAACAAGGGAAACTGTGAATTTTTAAGTTTAATTTCACATGACATATTGAATTGAACTAATTTTTAAATGTTACTCCCTTGAAAg                                                                                                                                                                                                                                                  <  1:136827/251‑1 (MQ=255)
 atGCTTTAACAACGTCGATTTACCGACCTGTCTCGCACCGGTGACAAGAACTGCCGGAAATTGCTTAAGTAGACGACTTACTAAAGGTTGGATTTGGCGGGAAAGGTACATATTCACTCTCAAAATTAGTTTAACTCAATACAGCATATTAAATTAAACTAATTTAAACAAGGGAAACTGTGAATTTTTAAGTTTAATTTCACATGACATATTGAATTGAACTAATTTTTAAATGTTACTCCCTTGAAAGt                                                                                                                                                                                                                                                 >  1:697341/1‑251 (MQ=255)
                    ttACCGACCTGTCTCGCACCGGTGACAAGAACTGCCGGAAATTGCTTAAGTAGACGACTTACTAAAGGTTGGATTTGGCGGGAAAGGTACATATTCACTCTCAAAATTAGTTTAACTCAATACAGCATATTAAATTAAACTAATTTAAACAAGGGAAACTGTGAATTTTTAAGTTTAATTTCACATGACATATTGAATTGAACTAATTTTTAAATGTTACTCCCTTGAAAGTGCGGTTGTTTTCTCAGGCa                                                                                                                                                                                                                              >  1:502696/1‑251 (MQ=255)
                         gACCTGTCTCGCACCGGTGACAAGAACTGCCGGAAATTGCTTAAGTAGACGACTTACTAAAGGTTGGATTTGGCGGGAAAGGTACATATTCACTCTCAAAATTAGTTTAACTCAATACAGCATATTAAATTAAACTAATTTAAACAAGGGAAACTGTGAATTTTTAAGTTTAATTTCACATGACATATTGAATTGAACTAATTTTTAAATGTTACTCCCTTGAAAGTGCGGTTGTTTTCTCAGGCAttttt                                                                                                                                                                                                                         >  1:787711/1‑251 (MQ=255)
                                                gAACTGCCGGAAATTGCTTAAGTAGACGACTTACTAAAGGTTGGATTTGGCGGGAAAGGTACATATTCACTCTCAAAATTAGTTTAACTCAATACAGCATATTAAATTAAACTAATTTAAACAAGGGAAACTGTGAATTTTTAAGTTTAATTTCACATGACATATTGAATTGAACTAATTTTTAAATGTTACTCCCTTGAAAg                                                                                                                                                                                                                                                  <  1:726485/203‑1 (MQ=255)
                                                 aaCTGCCGGAAATTGCTTAAGTAGACGACTTACTAAAGGTTGGATTTGGCGGGAAAGGTACATATTCACTCTCAAAATTAGTTTAACTCAATACAGCATATTAAATTAAACTAATTTAAACAAGGGAAACTGTGAATTTTTAAGTTTAATTTCACATGACATATTGAATTGAACTAATTTTTAAATGTTACTCCCTTGAAAGTGCGGTTGTTTTCTCAGGCATTTTTTCCTCACGTTCCGGCAAATACGcc                                                                                                                                                                                                 <  1:701855/251‑1 (MQ=255)
                                                               gCTTAAGTAGACGACTTACTAAAGGTTGGATTTGGCGGGAAAGGTACATATTCACTCTCAAAATTAGTTTAACTCAATACAGCATATTAAATTAAACTAATTTAAACAAGGGAAACTGTGAATTTTTAAGTTTAATTTCACATGACATATTGAATTGAACTAATTTTTAAATGTTACTCCCTTGAAAGTGCGGTTGTTTTCTCAGGCATTTTTTCCTCACGTTCCGGCa                                                                                                                                                                                                         >  1:516676/1‑229 (MQ=255)
                                                                   aaGTAGACGACTTACTAAAGGTTGGATTTGGCGGGAAAGGTACATATTCACTCTCAAAATTAGTTTAACTCAATACAGCATATTAAATTAAACTAATTTAAACAAGGGAAACTGTGAATTTTTAAGTTTAATTTCACATGACATATTGAATTGAACTAATTTTTAAATGTTACTCCCTTGAAAGTGCGGTTGTTTTCTCAGGCATTTTTTCCTCACGTTCCGGCAAATACGCCTTCAATCCACGTTCgcgc                                                                                                                                                                               <  1:201156/251‑1 (MQ=255)
                                                                            acTTACTAAAGGTTGGATTTGGCGGGAAAGGTACATATTCACTCTCAAAATTAGTTTAACTCAATACAGCATATTAAATTAAACTAATTTAAACAAGGGAAACTGTGAATTTTTAAGTTTAATTTCACATGACATATTGAATTGAACTAATTTTTAAATGTTACTCCCTTGAAAGTGCGGTTGTTTTCTCAGGCATTTTTTCCTCACGTTCCGGCAAATACGCCTTCAATCCACGTTCGCGCAAAACGCaa                                                                                                                                                                      >  1:304829/1‑251 (MQ=255)
                                                                            acTTACTAAAGGTTGGATTTGGCGGGAAAGGTACATATTCACTCTCAAAATTAGTTTAACTCAATACAGCATATTAAATTAAACTAATTTAAACAAGGGAAACTGTGAATTTTTAAGTTTAATTTCACATGACATATTGAATTGAACTAATTTTTAAATGTTACTCCCTTGAAAGTGCGGTTGTTTTCTCAGGCATTTTTTCCTCACGTTCCGGCAAATACGCCTTCAATCCACGTTCGCGCAAAACGCaa                                                                                                                                                                      >  1:889504/1‑251 (MQ=255)
                                                                                                         ggTACATATTCACTCTCAGAATTAGTTTAACTCAATACAGCATATTAAATTAAACTAATTTAAACAAGGGAAACTGTGAATTTTTAAGTTTAATTTCACATGACATATTGAATTGAACTAATTTTTAAATGTTACTCCCTTGAAAGTGCGGTTGTTTTCTCAGGCATTTTTTCCTCACGTTCCGGCAAATACGCCTTCAATCCACGTTCGCGCAAAACGCAACTCGGGCATTCGCCGCAACCGCCTTCGa                                                                                                                                          >  1:573585/1‑250 (MQ=255)
                                                                                                         ggTACATATTCACTCTCAAAATTAGTTTAACTCAATACAGCATATTAAATTAAACTAATTTAAACAAGGGAAACTGTGAATTTTTAAGTTTAATTTCACATGACATATTGAATTGAACTAATTTTTAAATGTTACTCCCTTGAAAGTGCGGTTGTTTTCTCAGGCATTTTTTCCTCACGTTCCGGCAAATACGCCTTCAATCCACGtt                                                                                                                                                                                    >  1:526273/1‑208 (MQ=255)
                                                                                                          gTACATATTCACTCTCAAAATTAGTTTAACTCAATACAGCATATTAAATTAAACTAATTTAAACAAGGGAAACTGTGAATTTTTAAGTTTAATTTCACATGACATATTGAATTGAACTAATTTTTAAATGTTACTCCCTTGAAAGTGCGGTTGTTTTCTCAGGCATTTTTTCCTCACGTTCCGGCAAATACGCCTTCAATCCACGTTCGCGCAAAACGCAACTCGGGCATTCGCCGCAACCGCCTTCGaca                                                                                                                                        >  1:326177/1‑251 (MQ=255)
                                                                                                           tACATATTCACTCTCAAAATTAGTTTAACTCAATACAGCATATTAAATTAAACTAATTTAAACAAGGGAAACTGTGAATTTTTAAGTTTAATTTCACATGACATATTGAATTGAACTAATTTTTAAATGTTACTCCCTTGAAAGTGCGGTTGTTTTCTCAGGCATTTTTTCCTCACGTTCCGGCAAATACGCCTTCAATCCACGTTCGCGCAAAACGCAACTCGGGCATTCGCCGCAACCGCCTTCGacac                                                                                                                                       <  1:896977/251‑1 (MQ=255)
                                                                                                                         cAAAATTAGTTTAACTCAATACAGCATATTAAATTAAACTAATTTAAACAAGGGAAACTGTGAATTTTTAAGTTTAATTTCACATGACATATTGAATTGAACTAATTTTTAAATGTTACTCCCTTGAAAGTGCGGTTGTTTTCTCAGGCATTTTTTCCTCACGTTCCGGCAAATACGCCTTCAATCCACGTTCGCGCAAAACGCAACTCGGGCATTCGCCGCAACCGCCTTCGACACCGTTGTAGCAAgtg                                                                                                                         <  1:378085/251‑1 (MQ=255)
                                                                                                                                     aaCTCAATACAGCATATTAAATTAAACTAATTTAAACAAGGGAAACTGTGAATTTTTAAGTTTAATTTCACATGACATATTGAATTGAACTAATTTTTAAATGTTACTCCCTTGAAAGTGCGGTTGTTTTCTCAGGCATTTTTTCCTCACGTTCCGGCAAATACGCCTTCAATCCACGTTCGCGCAAAACGCAACTCGGGCATTCGCCGCAACCGCCTTCGACACCGTTGTAGCAAGTGTGGGTGTGCcgg                                                                                                             >  1:299490/1‑251 (MQ=255)
                                                                                                                                      aCTCAATACAGCATATTAAATTAAACTAATTTAAACAAGGGAAACTGTGAATTTTTAAGTTTAATTTCACATGACATATTGAATTGAACTAATTTTTAAATGTTACTCCCTTGAAAGTGCGGTTGTTTTCTCAGGCATTTTTTCCTCACGTTCCGGCAAATACGCCTTCGATCCACGTTCGCGCAAAACGCAACTCGGGCATTcgc                                                                                                                                                         >  1:452028/1‑206 (MQ=255)
                                                                                                                                      aCTCAATACAGCATATTAAATTAAACTAATTTAAACAAGGGAAACTGTGAATTTTTAAGTTTAATTTCACATGACATATTGAATTGAACTAATTTTTAAATGTTACTCCCTTGAAAGTGCGGTTGTTTTCTCAGGCATTTTTTCCTCACGTTCCGGCAAATACGCCTTCAATCCACGTTCGCGCAAAACGCAACTCGGGCATTCGCCGCAACCGCCTTCGACACCGTTGTAGCAAGTGTGGGTGTGCcgg                                                                                                             >  1:79510/1‑250 (MQ=255)
                                                                                                                                      aCTCAATACAGCATATTAAATTAAACTAATTTAAACAAGGGAAACTGTGAATTTTTAAGTTTAATTTCACATGACATATTGAATTGAACTAATTTTTAAATGTTACTCCCTTGAAAGTGCGGTTGTTTTCTCAGGCATTTTTTCCTCACGTTCCGGCAAATACGCCTTCAATCCACGTTCGCGCAAAACGCAACTCGGGCATTCGCCGCAACCGCCTTCGACACCGTTGTAGCAAGTGTGGGTGTGCcgg                                                                                                             >  1:545733/1‑250 (MQ=255)
                                                                                                                                         cAATACAGCATATTAAATTAAACTAATTTAAACAAGGGAAACTGTGAATTTTTAAGTTTAATTTCACATGACATATTGAATTGAACTAATTTTTAAATGTTACTCCCTTGAAAGTGCGGTTGTTTTCTCAGGCATTTTTTCCTCACGTTCCGGCAAATACGCCTTCAATCCACGTTCGCGCAAAACGCAACTCGGGCATTCGCCGCAAc                                                                                                                                                   >  1:545243/1‑209 (MQ=255)
                                                                                                                                         cAATACAGCATATTAAATTAAACTAATTTAAACAAGGGAAACTGTGAATTTTTAAGTTTAATTTCACATGACATATTGAATTGAACTAATTTTTAAATGTTACTCCCTTGAAAGTGCGGTTGTTTTCTCAGGCATTTTTTCCTCACGTTCCGGCAAATACGCCTTCAATCCACGTTCGCGCAAAACGCAACTCGGGCATTCGCCGCAACCGCCTTCGacac                                                                                                                                       <  1:106673/221‑1 (MQ=255)
                                                                                                                                         cAATACAGCATATTAAATTAAACTAATTTAAACAAGGGAAACTGTGAATTTTTAAGTTTAATTTCACATGACATATTGAATTGAACTAATTTTTAAATGTTACTCCCTTGAAAGTGCGGTTGTTTTCTCAGGCATTTTTTCCTCACGTTCCGGCAAATACGCCTTCAATCCACGTTCGCGCAAAACGCAACTCGGGCATTCGCCGCAACCGCCTTCGACACCGTTGTAGCAAGTGTGGGTGTGCCGGCGGa                                                                                                         >  1:132182/1‑251 (MQ=255)
                                                                                                                                         cAATACAGCATATTAAATTAAACTAATTTAAACAAGGGAAACTGTGAATTTTTAAGTTTAATTTCACATGACATATTGAATTGAACTAATTTTTAAATGTTACTCCCTTGAAAGTGCGGTTGTTTTCTCAGGCATTTTTTCCTCACGTTCCGGCAAATACGCCTTCAATCCACGTTCGCGCAAAACGCAACTCGGGCATTCGCCGCAACCGCCTTCGACACCGTTGTAGCAAGTGTGGGTGTGCCGGCGGa                                                                                                         >  1:108031/1‑251 (MQ=255)
                                                                                                                                           aTACAGCATATTAAATTAAACTAATTTAAACAAGGGAAACTGTGAATTTTTAAGTTTAATTTCACATGACATATTGAATTGAACTAATTTTTAAATGTTACTCCCTTGAAAGTGCGGTTGTTTTCTCAGGCATTTTTTCCTCACGTTCCGGCAAATACGCCTTCAATCCACg                                                                                                                                                                                      >  1:869290/1‑172 (MQ=255)
                                                                                                                                           aTACAGCATATTAAATTAAACTAATTTAAACAAGGGAAACTGTGAATTTTTAAGTTTAATTTCACATGACATATTGAATTGAACTAATTTTTAAATGTTACTCCCTTGAAAGTGCGGTTGTTTTCTCAGGCATTTTTTCCTCACGTTCCGGCAAATACGCCTTCAATCCACGTTCGCGCAAAACGCAACTCGGGCATTCGCCGCAACCGCCTTCGACACCGTTGTAGCa                                                                                                                             >  1:877326/1‑229 (MQ=255)
                                                                                                                                                gCATATTAAATTAAACTAATTTAAACAAGGGAAACTGTGAATTTTTAAGTTTAATTTCACATGACATATTGAATTGAACTAATTTTTAAATGTTACTCCCTTGAAAGTGCGGTTGTTTTCTCAGGCATTTTTTCCTCACGTTCCGGCAAATACGCCTTCAATCCACGTTCGCGCAAAACGCAACTCGGGCATTCGCCGCAACCGCCTTCGACACCGTTGTAGCa                                                                                                                             <  1:865188/224‑1 (MQ=255)
                                                                                                                                                gCATATTAAATTAAACTAATTTAAACAAGGGAAACTGTGAATTTTTAAGTTTAATTTCACATGACATATTGAATTGAACTAATTTTTAAATGTTACTCCCTTGAAAGTGCGGTTGTTTTCTCAGGCATTTTTTCCTCACGTTCCGGCAAATACGCCTTCAATCCACGTTCGCGCAAAACGCAACTCGGGCATTCGCCGCAACCGCCTTCGACACCGTTGTAGCAAGTGTGGGTGTGCCGGCGGATATAAt                                                                                                   >  1:88163/1‑250 (MQ=255)
                                                                                                                                                 cATATTAAATTAAACTAATTTAAACAAGGGAAACTGTGAATTTTTAAGTTTAATTTCACATGACATATTGAATTGAACTAATTTTTAAATGTTACTCCCTTGAAAGTGCGGTTGTTTTCTCAGGCATTTTTTCCTCACGTTCCGGCAAATACGCCTTCAATCCACGTTCGCGCAAAACGCAACTCGGGCATTCGCCGCAACCGCCTTCGACACCGTTGTAGCAAGTGTGGGTGTGCCGGCGGATATAATc                                                                                                  <  1:812988/250‑1 (MQ=255)
                                                                                                                                                       aaattaaaCTAATTTAAACAAGGGAAACTGTGAATTTTTAAGTTTAATTTCACATGACATATTGAATTGAACTAATTTTTAAATGTTACTCCCTTGAAAGTGCGGTTGTTTTCTCAGGCATTTTTTCCTCACGTTCCGGCAAATACGCCTTCAATCCACGTTCGCGCAAAACGCAACTCGGGCATTCGCCGCAACCGCCTTCGACACCGTTGTAGCAAGTGTGGGTGTGCCGGCGGATATAATCAAAcgcg                                                                                           >  1:100095/1‑251 (MQ=255)
                                                                                                                                                              aCTAATTTAAACAAGGGAAACTGTGAATTTTTAAGTTTAATTTCACATGACATATTGAATTGAACTAATTTTTAAATGTTACTCCCTTGAAAGTGCGGTTGTTTTCTCAGGCATTTTTTCCTCACGTTCCGGCAAATACGCCTTCAATCCACGTTCGCGCAAAACGCAACTCGGGCATTCGCCGCAACCGCCTTCGACACCGTTGTAGCAAGTGTGGGTGTGCCGGCGGATATAATCAAACGCGCCAAGtt                                                                                    <  1:352803/251‑1 (MQ=255)
                                                                                                                                                                 aaTTTAAACAAGGGAAACTGTGAATTTTTAAGTTTAATTTCACATGACATATTGAATTGAACTAATTTTTAAATGTTACTCCCTTGAAAGTGCGGTTGTTTTCTCAGGCATTTTTTCCTCACGTTCCGGCAAATACGCCTTCAATCCACGTTCGCGCAAAACGCAACTCGGGCATTCGCCGCAACCGCCTTCGACACCGTTGTAGCAAGTGTGGGTGTGCCGGCGGATATAATCAAACGCGCCAAGTTGAt                                                                                 >  1:837003/1‑251 (MQ=255)
                                                                                                                                                                  aTTTAAACAAGGGAAACTGTGAATTTTTAAGTTTAATTTCACATGACATATTGAATTGAACTAATTTTTAAATGTTACTCCCTTGAAAGTGCGGTTGTTTTCTCAGGCATTTTTTCCTCACGTTCCGGCAAATACGCCTTCAATCCACGTTCGCGCAAAACGCAACTCGGGCATTCGCCGCAACCGCCTTCGACACCGTTGTAGCAAGTGTGGGTGTGCCGGCGGATATAATCAAACGCGCCAAGTTGATc                                                                                <  1:369427/251‑1 (MQ=255)
                                                                                                                                                                      aaaCAAGGGAAACTGTGAATTTTTAAGTTTAATTTCACATGACATATTGAATTGAACTAATTTTTAAATGTTACTCCCTTGAAAGTGCGGTTGTTTTCTCAGGCATTTTTTCCTCACGTTCCGGCAAATACGCCTTCAATCCACGTTCGCGCAAAACGCAACTCGGGCATTCGCCGCAACCGCCTTCGACACCGTTGTAGCAAGTGTGGGTGTGCCGGCGGATATAATCAAACGCGCCAAGTTGATCCGc                                                                             >  1:375132/1‑250 (MQ=255)
                                                                                                                                                                        aCAAGGGAAACTGTGAATTTTTAAGTTTAATTTCACATGACATATTGAATTGAACTAATTTTTAAATGTTACTCCCTTGAAAGTGCGGTTGTTTTCTCAGGCATTTTTTCCTCACGTTCCGGCAAATACGCCTTCAATCCACGTTCGCGCAAAACGCAACTCGGGCATTCGCCGCAACCGCCTTCGACACCGTTGTAGCAAGTGTGGGTGTGCCGGCGGatat                                                                                                      >  1:446533/1‑223 (MQ=255)
                                                                                                                                                                         cAAGGGAAACTGTGAATTTTTAAGTTTAATTTCACATGACATATTGAATTGAACTAATTTTTAAATGTTACTCCCTTGAAAGTGCGGTTGTTTTCTCAGGCATTTTTTCCTCACGTTCCGGCAAATACGCCTTCAATCCACGTTCGCGCAAAACGCAACTCGGGCATTCGCCGCAACCGCCTTCGACACCGTTGTAGCAAGTGTGGGTGTGCCGGCGGATATAATCAAACGCGCCAAGTTGATCCGCCAg                                                                          >  1:532460/1‑250 (MQ=255)
                                                                                                                                                                                                     aTTTCACATGACATATTGAATTGAACTAATTTTTAAATGTTACTCCCTTGAAAGTGCGGTTGTTTTCTCAGGCATTTTTTCCTCACGTTCCGGCAAATACGCCTTCAATCCACGTTCGCGCAAAACGCAACTCGGGCATTCGCCGCAACCGCCTTCGACACCGTTGTAGCa                                                                                                                             <  1:714255/171‑1 (MQ=255)
                                                                                                                                                                                                       ttCACATGACATATTGAATTGAACTAATTTTTAAATGTTACTCCCTTGAAAGTGCGGTTGTTTTCTCAGGCATTTTTTCCTCACGTTCCGGCAAATACGCCTTCAATCCACGTTCGCGCAAAACGCAACTCGGGCATTCGCCGCAACCGCCTTCGACACCGTTGTAGCAAGTGTGGGTGTGCCGGCGGATATAATCAAACGCGCCAAGTTGATCCGCCAGCGCCCAGGTTTCTTTTTTATCCAGCCACAtt                                           <  1:259947/251‑1 (MQ=255)
                                                                                                                                                                                                        tCACATGACATATTGAATTGAACTAATTTTTAAATGTTACTCCCTTGAAAGTGCGGTTGTTTTCTCAGGCATTTTTTCCTCACGTTCCGGCAAATACGCCTTCAATCCACGTTCGCGCAAAACGCAACTCGGGCATTCGCCGCAACCGCCTTCGACACCGTTGTAGCAAGTGTGGGTGTGCCGGCGGATATAATCAAACGCGCCAAGTTGATCCGcc                                                                            <  1:345701/217‑1 (MQ=255)
                                                                                                                                                                                                         cacaTGACATATTGAATTGAACTAATTTTTAAATGTTACTCCCTTGAAAGTGCGGTTGTTTTCTCAGGCATTTTTTCCTCACGTTCCGGCAAATACGCCTTCAATCCACGTTCGCGCAAAACGCAACTCGGGCATTCGCCGCAACCGCCTTCGACACCGTTGTAGCAAGTGTGGGTGTGCCGGCGGATATAATCAAACGCGCCAAGTTGATCCGCCAgc                                                                         <  1:802732/219‑1 (MQ=255)
                                                                                                                                                                                                         cacaTGACATATTGAATTGAACTAATTTTTAAATGTTACTCCCTTGAAAGTGCGGTTGTTTTCTCAGGCATTTTTTCCTCACGTTCCGGCAAATACGCCTTCAATCCACGTTCGCGCAAAACGCAACTCGGGCATTCGCCGCAACCGCCTTCGACACCGTTGTAGCAAGTGTGGGTGTGCCGGCGGATATAATCAAACGCGCCAAGTTGATCCGCCAGCGCCCAGGTTTCTTTTTTATCCAGCCACATTaa                                         >  1:173692/1‑251 (MQ=255)
                                                                                                                                                                                                            aTGACATATTGAATTGAACTAATTTTTAAATGTTACTCCCTTGAAAGTGCGGTTGTTTTCTCAGGCATTTTTTCCTCACGTTCCGGCAAATACGCCTTCAATCCACGTTCGCGCAAAACGCAACTCGGGCATTCGCCGCAACCGCCTTCGACACCGTTGTAGCAAGTGTGGGTGTGCCGGCGGATATAATCAAACGCGCCAAGTTGATCCGCCAGCGCCCAGGTTTCTTTTTTATCCAGCCACATTAACgg                                      <  1:270884/251‑1 (MQ=255)
                                                                                                                                                                                                            aTGACATATTGAATTGAACTAATTTTTAAATGTTACTCCCTTGAAAGTGCGGTTGTTTTCTCAGGCATTTTTTCCTCACGTTCCGGCAAATACGCCTTCAATCCACGTTCGCGCAAAACGCAACTCGGGCATTCGCCGCAACCGCCTTCGACACCGTTGTAGCAAGTGTGGGTGTGCCGGCGGATATAATCAAACGCGCCAAGTTGATCCGCCAGCGCCCAGGTTTCTTTTTTATCCAGCCACATTAACgg                                      <  1:654745/251‑1 (MQ=255)
                                                                                                                                                                                                            aTGACATATTGAATTGAACTAATTTTTAAATGTTACTCCCTTGAAAGTGCGGTTGTTTTCTCAGGCATTTTTTCCTCACGTTCCGGCAAATACGCCTTCAATCCACGTTCGCGCAAAACGCAACTCGGGCATTCGCCGCAACCGCCTTCGACACCGTTGTAGCAAGTGTGGGTGTGCCGGCGGATATAATCAAACGCGCCAAGTTGATCCGCCAGCGCCCAGGTTTCTTTTTTATCCAGCCACATTAACgg                                      <  1:16452/251‑1 (MQ=255)
                                                                                                                                                                                                            aTGACATATTGAATTGAACTAATTTTTAAATGTTACTCCCTTGAAAGTGCGGTTGTTTTCTCAGGCATTTTTTCCTCACGTTCCGGCAAATACGCCTTCAATCCACGTTCGCGCAAAACGCAACTCGGGCATTCGCCGCAACCGCCTTCGACACCGTTGTAGCAAGTGTGGGTGTGCCGGCGGATATAATCAAACGCGCCAAGTTGATCCGCCAGCGCCCAGGTTTCTTTTTTATCCAGCCACATTAACgg                                      <  1:760219/251‑1 (MQ=255)
                                                                                                                                                                                                               aCATATTGAATTGAACTAATTTTTAAATGTTACTCCCTTGAAAGTGCGGTTGTTTTCTCAGGCATTTTTTCCTCACGTTCCGGCAAATACGCCTTCAATCCACGTTCGCGCAAAACGCAACTCGGGCATTCGCCGCAACCGCCTTCGACACCGTTGTAGCAAGTGTGGGTGTGCCGGCGGATATAATCAAACGCGCCAAGTTGATCCGCCAGCGCCCAGGTTTCTTTTTTATCCAGCCACATTAACggagt                                   >  1:638129/1‑248 (MQ=255)
                                                                                                                                                                                                               aCATATTGAATTGAACTAATTTTTAAATGTTACTCCCTTGAAAGTGCGGTTGTTTTCTCAGGCATTTTTTCCTCACGTTCCGGCAAATACGCCTTCAATCCACGTTCGCGCAAAACGCAACTCGGGCATTCGCCGCAACCGCCTTCGACACCGTTGTAGCAAGTGTGGGTGTGCCGGCGGATATAATCAAACGCGCCAAGTTGATCCGCCAGCGCCCAGGTTTCTTTTTTATCCAGCCACATTAACGgtgt                                   >  1:880123/1‑251 (MQ=255)
                                                                                                                                                                                                                 atatTGAATTGAACTAATTTTTAAATGTTACTCCCTTGAAAGTGCGGTTGTTTTCTCAGGCATTTTTTCCTCACGTTCCGGCAAATACGCCTTCAATCCACg                                                                                                                                                                                      <  1:872546/102‑1 (MQ=255)
                                                                                                                                                                                                                 atatTGAATTGAACTAATTTTTAAATGTTACTCCCTTGAAAGTGCGGTTGTTTTCTCAGGCATTTTTTCCTCACGTTCCGGCAAATACGCCTTCAATCCACGTTCGCGCAAAACGCAACTCGGGCATTCGCCGCAACCGCCTTCGACACCGTTGTAGCAAGTGTGGGTGTGCCGGCGGATATAATCAAACGCGCCAAGTTGATCCGCCAGCGCCCAGGTTTCTTTTTTATCCAGCCACATTAACgg                                      <  1:93893/246‑1 (MQ=255)
                                                                                                                                                                                                                 atatTGAATTGAACTAATTTTTAAATGTTACTCCCTTGAAAGTGCGGTTGTTTTCTCAGGCATTTTTTCCTCACGTTCCGGCAAATACGCCTTCAATCCACGTTCGCGCAAAACGCAACTCGGGCATTCGCCGCAACCGCCTTCGACACCGTTGTAGCAAGTGTGGGTGTGCCGGCGGATATAATCAAACGCGCCAAGTTGATCCGCCAGCGCCCAGGTTTCTTTTTTATCCAGCCACATTAACgg                                      <  1:38932/246‑1 (MQ=255)
                                                                                                                                                                                                                   attgaattgaACTAATTTTTAAATGTTACTCCCTTGAAAGTGCGGTTGTTTTCTCAGGCATTTTTTCCTCACGTTCCGGCAAATACGCCTTCAATCCACGTTCGCGCAAAACGCAACTCGGGCATTCGCCGCAACCGCCTTCGACACCGTTGTAGCAAGTGTGGGTGTGCCGGCGGATATAATCAAACGCGCCAAGTTGATCCGCCAGCGCCCAGGTTTCTTTTTTATCCAGCCACATTAACgg                                      <  1:771130/244‑1 (MQ=255)
                                                                                                                                                                                                                      gaattgaaCTAATTTTTAAATGTTACTCCCTTGAAAGTGCGGTTGTTTTCTCAGGCATTTTTTCCTCACGTTCCGGCAAATACGCCTTCAATCCACGTTCGCGCAAAACGCAACTCGGGCATTCGCCGCAACCGCCTTCGACACCGTTGTAGCa                                                                                                                             >  1:298014/1‑154 (MQ=255)
                                                                                                                                                                                                                      gaattgaaCTAATTTTTAAATGTTACTCCCTTGAAAGTGCGGTTGTTTTCTCAGGCATTTTTTCCTCACGTTCCGGCAAATACGCCTTCAATCCACGTTCGCGCAAAACGCAACTCGGGCATTCGCCGCAACCGCCTTCGACACCGTTGTAGCAAGTGTGGGTGTGCCGGCGGATATAATCAAACGCGCCAAGTTGATCCGCCAGCGCCCAGGTTTCTTTTTTATCCAGCCACATTAACgg                                      >  1:477633/1‑241 (MQ=255)
                                                                                                                                                                                                                            aaCTAATTTTTAAATGTTACTCCCTTGAAAGTGCGGTTGTTTTCTCAGGCATTTTTTCCTCACGTTCCGGCAAATACGCCTTCAATCCACGTTCGCGCAAAACGCAACTCGGGCATTCGCCGCAACCGCCTTCGACACCGTTGTAGCAAGTGTGGGTGTGCCGGCGGATATAATCAAACGCGCCAAGTTGATCCGCCAGCGCCCAGGTTTCTTTTTTATCCAGCCACATTAACGGTGTGCGGATTTCaaa                       <  1:502004/250‑1 (MQ=255)
                                                                                                                                                                                                                                  tttttAAATGTTACTCCCTTGAAAGTGCGGTTGTTTTCTCAGGCATTTTTTCCTCACGTTCCGGCAAATACGCCTTCAATCCACGTTCGCGCAAAACGCAACTCGGGCATTCGCCGCAACCGCCTTCGACACCGTTGTAGCa                                                                                                                             <  1:204096/142‑1 (MQ=255)
                                                                                                                                                                                                                                  tttttAAATGTTACTCCCTTGAAAGTGCGGTTGTTTTCTCAGGCATTTTTTCCTCACGTTCCGGCAAATACGCCTTCAATCCACGTTCGCGCAAAACGCAACTCGGGCATTCGCCGCAACCGCCTTCGACACCGTTGTAGCAAGTGTGGGTGTGCCGGCGGATATAATCAAACGCGCCAAGTTGATCCGCCAGCGCCCAGGTTTCTTTTTTATCCAGCCACATTAACgg                                      >  1:145501/1‑229 (MQ=255)
                                                                                                                                                                                                                                   ttttAAATGTTACTCCCTTGAAAGTGCGGTTGTTTTCTCAGGCATTTTTTCCTCACGTTCCGGCAAATACGCCTTCAATCCACGTTCGCGCAAAACGCAACTCGGGCATTCGCCGCAACCGCCTTCGACACCGTTGTAGCAAGTGTggg                                                                                                                     >  1:239969/1‑149 (MQ=255)
                                                                                                                                                                                                                                         aTGTTACTCCCTTGAAAGTGCGGTTGTTTTCTCAGGCATTTTTTCCTCACGTTCCGGCAAATACGCCTTCAATCCACGTTCGCGCAAAACGCAACTCGGGCATTCGCCGCAACCGCCTTCGACACCGTTGTAGCAAGTGTGGGTGTGCCGGCGGATATAATCAAACGCGCCAAGTTGATCCGCCAGCGCCCAGGTTTCTTTTTTATCCAGCCACATTAACgg                                      <  1:320178/222‑1 (MQ=255)
                                                                                                                                                                                                                                         aTGTTACTCCCTTGAAAGTGCGGTTGTTTTCTCAGGCATTTTTTCCTCACGTTCCGGCAAATACGCCTTCAATCCACGTTCGCGCAAAACGCAACTCGGGCATTCGCCGCAACCGCCTTCGACACCGTTGTAGCAAGTGTGGGTGTGCCGGCGGATATAATCAAACGCGCCAAGTTGATCCGCCAGCGCCCAGGTTTCTTTTTTATCCAGCCACATTAACgg                                      >  1:295952/1‑222 (MQ=255)
                                                                                                                                                                                                                                         aTGTTACTCCCTTGAAAGTGCGGTTGTTTTCTCAGGCATTTTTTCCTCACGTTCCGGCAAATACGCCTTCAATCCACGTTCGCGCAAAACGCAACTCGGGCATTCGCCGCAACCGCCTTCGACACCGTTGTAGCAAGTGTGGGTGTGCCGGCGGATATAATCAAACGCGCCAAGTTGATCCGCCAGCGCCCAGGTTTCTTTTTTATCCAGCCACATTAACGGTGTGCGGATTTCAAAAGGGTAAGCCATgg         >  1:88001/1‑251 (MQ=255)
                                                                                                                                                                                                                                          tGTTACTCCCTTGAAAGTGCGGTTGTTTTCTCAGGCATTTTTTCCTCACGTTCCGGCAAATACGCCTTCAATCCACGTTCGCGCAAAACGCAACTCGGGCATTCGCCGCAACCGCCTTCGACACCGTTGTAGCAAGTGTGGGTGTGCCGGCGGATATAATCAAACGCGCCAAGTTGATCCGCCAGCGCCCAGGTTTCTTTTTTATCCAGCCACATTAACGGTGTGCGGATTTCAAAAGGGTAAGcc             <  1:293831/246‑1 (MQ=255)
                                                                                                                                                                                                                                                tCCCTTGAAAGTGCGGTTGTTTTCTCAGGCATTTTTTCCTCACGTTCCGGCAAATACGCCTTCAATCCACGTTCGCGCAAAACGCAACTCGGGCATTCGCCGCAACCGCCTTCGACACCGTTGTAGCAAGTGTGGGTGTGCCGGCGGATATAATCAAACGCGCCAAGTTGATCCGCCAGCGCCCAGGTTTCTTTTTTATCCAGCCACATTAACGGTGTGCGGATTTCAAAAGGGTAAGCCATGGCAAGGtt  <  1:677499/251‑1 (MQ=255)
                                                                                                                                                                                                                                                 cccTTGAAAGTGCGGTTGTTTTCTCAGGCATTTTTTCCTCACGTTCCGGCAAATACGCCTTCAATCCACGTTCGCGCAAAACGCAACTCGGGCATTCGCCGCAAc                                                                                                                                                   <  1:478468/105‑1 (MQ=255)
                                                                                                                                                                                                                                                 cccTTGAAAGTGCGGTTGTTTTCTCAGGCATTTTTTCCTCACGTTCCGGCAAATACGCCTTCAATCCACGTTCGCGCAAAACGCAACTCGGGCATTCGCCGCAACCGCCTTCGACACCGTTGTAGCAAGTGTGGGTGTGCCGGCGGATATAATc                                                                                                  >  1:243966/1‑154 (MQ=255)
                                                                                                                                                                                                                                                 cccTTGAAAGTGCGGTTGTTTTCTCAGGCATTTTTTCCTCACGTTCCGGCAAATACGCCTTCAATCCACGTTCGCGCAAAACGCAACTCGGGCATTCGCCGCAACCGCCTTCGACACCGTTGTAGCAAGTGTGGGTGTGCCGGCGGATATAATCAAACGCGCCAAGTTGATCCGCCAGCGCCCAGGTTTCTTTTTTATCCAGCCACATTAACGGTGTGCGGATTTCAAAAGGGTAAGcc             >  1:208117/1‑239 (MQ=255)
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                     
TATGCTTTAACAACGTCGATTTACCGACCTGTCTCGCACCGGTGACAAGAACTGCCGGAAATTGCTTAAGTAGACGACTTACTAAAGGTTGGATTTGGCGGGAAAGGTACATATTCACTCTCAAAATTAGTTTAACTCAATACAGCATATTAAATTAAACTAATTTAAACAAGGGAAACTGTGAATTTTTAAGTTTAATTTCACATGACATATTGAATTGAACTAATTTTTAAATGTTACTCCCTCGAAAGTGCGGTTGTTTTCTCAGGCATTTTTTCCTCACGTTCCGGCAAATACGCCTTCAATCCACGTTCGCGCAAAACGCAACTCGGGCATTCGCCGCAACCGCCTTCGACACCGTTGTAGCAAGTGTGGGTGTGCCGGCGGATATAATCAAACGCGCCAAGTTGATCCGCCAGCGCCCAGGTTTCTTTTTTATCCAGCCACATTAACGGTGTGCGGATTTCAAAAGGGTAAGCCATGGCAAGGTT  >  1/1435316‑1435806

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑