Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	190,963	G→T	intergenic (‑130/‑65)	unknown ← / → unknown	Transcriptional regulator for fatty acid degradation FadR, GntR family/Na+/H+ antiporter NhaB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	190,963	0	G	T	100.0%	126.4 / NA	45	intergenic (‑130/‑65)	unknown/unknown	Transcriptional regulator for fatty acid degradation FadR, GntR family/Na+/H+ antiporter NhaB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (14/31);  total (14/31)

GGTAAATCCGAACCGGGTGGGAAATGATTATTCCAAATGCTTTTTACAATGTATTCTTCTGCTAATGCCGCCGGGCTTTGTGCTTTCAGAAGGGGGGAATTGTTATTCATAGTAATTCGGTATTATCAGTAGTTATCAAATAAAAAACGGCTTTATTATCGTTGAAACGACTATACATTACAATTAGATAATTATTTTTTAAATTCGTTGACATCATCTGCTATTTTTGACACAGTTTAGCGCTGAACTACCTTGAACGGTATTAGGTTAATATATTGTTAATTAAGAAATTAAGGTCAACTTTATGGATAGTAATTATACACAGGCATTTTTTAAAAACTTTCTTGGTTCTAGCCCGGGCTGGTATAAAATAGCGATCGTCGCATTTCTTGTCATTAATCCCGTCTTATTTGCTATTTCACCTTTTTGGGCGGGGTGGTTATTAGTCATTGAATTCATTTTCACCCTAGCTATGGCGCTAAAAT  >  1/190724‑191208                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                      ggTAAATCCGAACCGGGTGGGAAATGATTATTCCAAATGCTTTTTACAATGTATTCTTCTGCTAATGCCGCCGGGCTTTGTGCTTTCAGAAGGGGGGAATTGTTATTCATAGTAATTCGGTATTATCAGTAGTTATCAAATAAAAAACGGCTTTATTATCGTTGAAACGACTATACATTACAATTAGATAATTATTTTTTAAATTCGTTGACATCATCTGCTATTTTTGACACAGTTTATCGCTGAACTa                                                                                                                                                                                                                                             >  1:153258/1‑250 (MQ=255)  gTAAATCCGAACCGGGTGGGAAATGATTATTCCAAATGCTTTTTACAATGTATTCTTCTGCTAATGCCGCCGGGCTTTGTGCTTTCAGAAGGGGGGAATTGTTATTCATAGTAATTCGGTATTATCAGTAGTTATCAAATAAAAAACGGCTTTATTATCGTTGAAACGACTATACATTACAATTAGATAATTATTTTTTAAATTCGTTGACATCATCTGCTATTTTTGACACAGTTTATCGCTGAACTAc                                                                                                                                                                                                                                            <  1:658215/250‑1 (MQ=255)        ccGAACCGGGTGGGAAATGATTATTCCAAATGCTTTTTACAATGTATTCTTCTGCTAATGCCGCCGGGCTTTGTGCTTTCAGAAGGGGGGAATTGTTATTCATAGTAATTCGGTATTATCAGTAGTTATCAAATAAAAAACGGCTTTATTATCGTTGAAACGACTATACATTACAATTAGATAATTATTTTTTAAATTCGTTGACATCATCTGCTATTTTTGACACAGTTTATCGCTGAACTACCTTGAAc                                                                                                                                                                                                                                     <  1:889408/251‑1 (MQ=255)                  tGGGAAATGATTATTCCAAATGCTTTTTACAATGTATTCTTCTGCTAATGCCGCCGGGCTTTGTGCTTTCAGAAGGGGGGAATTGTTATTCATAGTAATTCGGTATTATCAGTAGTTATCAAATAAAAAACGGCTTTATTATCGTTGAAACGACTATACATTACAATTAGATAATTATTTTTTAAATTCGTTGACATCATCTGCTATTTTTGACACAGTTTATCGCTGAACTACCTTGAACGGTATTAGGt                                                                                                                                                                                                                           <  1:211013/251‑1 (MQ=255)                  tGGGAAATGATTATTCCAAATGCTTTTTACAATGTATTCTTCTGCTAATGCCGCCGGGCTTTGTGCTTTCAGAAGGGGGGAATTGTTATTCATAGTAATTCGGTATTATCAGTAGTTATCAAATAAAAAACGGCTTTATTATCGTTGAAACGACTATACATTACAATTAGATAATTATTTTTTAAATTCGTTGACATCATCTGCTATTTTTGACACAGTTTATCGCTGAACTACCTTGAACGGTATTAGGt                                                                                                                                                                                                                           <  1:604670/251‑1 (MQ=255)                  tGGGAAATGATTATTCCAAATGCTTTTTACAATGTATTCTTCTGCTAATGCCGCCGGGCTTTGTGCTTTCAGAAGGGGGGAATTGTTATTCATAGTAATTCGGTATTATCAGTAGTTATCAAATAAAAAACGGCTTTATTATCGTTGAAACGACTATACATTACAATTAGATAATTATTTTTTAAATTCGTTGACATCATCTGCTATTTTTGACACAGTTTATCGCTGAACTACCTTGAACGGTATTAGGt                                                                                                                                                                                                                           <  1:642199/251‑1 (MQ=255)                  tGGGAAATGATTATTCCAAATGCTTTTTACAATGTATTCTTCTGCTAATGCCGCCGGGCTTTGTGCTTTCAGAAGGGGGGAATTGTTATTCATAGTAATTCGGTATTATCAGTAGTTATCAAATAAAAAACGGCTTTATTATCGTTGAAACGACTATACATTACAATTAGATAATTATTTTTTAAATTCGTTGACATCATCTGCTATTTTTGACACAGTTTATCGCTGAACTACCTTGAACGGTATTAGGt                                                                                                                                                                                                                           <  1:334352/251‑1 (MQ=255)                  tGGGAAATGATTATTCCAAATGCTTTTTACAATGTATTCTTCTGCTAATGCCGCCGGGCTTTGTGCTTTCAGAAGGGGGGAATTGTTATTCATAGTAATTCGGTATTATCAGTAGTTATCAAATAAAAAACGGCTTTATTATCGTTGAAACGACTATACATTACAATTAGATAATTATTTTTTAAATTCGTTGACATCATCTGCTATTTTTGACACAGTTTATCGCTGAACTACCTTGAACGGTATTAGGt                                                                                                                                                                                                                           <  1:732350/251‑1 (MQ=255)                  tGGGAAATGATTATTCCAAATGCTTTTTACAATGTATTCTTCTGCTAATGCCGCCGGGCTTTGTGCTTTCAGAAGGGGGGAATTGTTATTCATAGTAATTCGGTATTATCAGTAGTTATCAAATAAAAAACGGCTTTATTATCGTTGAAACGACTATACATTACAATTAGATAATTATTTTTTAAATTCGTTGACATCATCTGCTATTTTTGACACAGTTTATCGCTGAACTACCTTGAACGGTATTAGGt                                                                                                                                                                                                                           <  1:763985/251‑1 (MQ=255)                  tGGGAAATGATTATTCCAAATGCTTTTTACAATGTATTCTTCTGCTAATGCCGCCGGGCTTTGTGCTTTCAGAAGGGGGGAATTGTTATTCATAGTAATTCGGTATTATCAGTAGTTATCAAATAAAAAACGGCTTTATTATCGTTGAAACGACTATACATTACAATTAGATAATTATTTTTTAAATTCGTTGACATCATCTGCTATTTTTGACACAGTTTATCGCTGAACTACCTTGAACGGTATTAGGt                                                                                                                                                                                                                           <  1:15570/251‑1 (MQ=255)                  tGGGAAATGATTATTCCAAATGCTTTTTACAATGTATTCTTCTGCTAATGCCGCCGGGCTTTGTGCTTTCAGAAGGGGGGAATTGTTATTCATAGTAATTCGGTATTATCAGTAGTTATCAAATAAAAAACGGCTTTATTATCGTTGAAACGACTATACATTACAATTAGATAATTATTTTTTAAATTCGTTGACATCATCTGCTATTTTTGACACAGTTTATCGCTGAACTACCTTGAACGGTATTAGGt                                                                                                                                                                                                                           <  1:142892/251‑1 (MQ=255)                     gAAATGATTATTCCAAATGCTTTTTACAATGTATTCTTCTGCTAATGCCGCCGGGCTTTGTGCTTTCAGAAGGGGGGAATTGTTATTCATAGTAATTCGGTATTATCAGTAGTTATCAAATAAAAAACGGCTTTATTATCGTTGAAACGACTATACATTACAATTAGATAATTATTTTTTAAATTCGTTGACATCATCTGCTATTTTTGACACAGTTTATCGCTGAACTACCTTGAACGGTATTAGGt                                                                                                                                                                                                                           <  1:94295/248‑1 (MQ=255)                        aTGATTATTCCAAATGCTTTTTACAATGTATTCTTCTGCTAATGCCGCCGGGCTTTGTGCTTTCAGAAGGGGGGAATTGTTATTCATAGTAATTCGGTATTATCAGTAGTTATCAAATAAAAAACGGCTTTATTATCGTTGAAACGACTATACATTACAATTAGATAATTATTTTTTAAATTCGTTGACATCATCTGCTATTTTTGACACAGTTTATCGCTGAACTACCTTGAACGGTATTAGGTTAatat                                                                                                                                                                                                                     <  1:181997/251‑1 (MQ=255)                             tattCCAAATGCTTTTTACAATGTATTCTTCTGCTAATGCCGCCGGGCTTTGTGCTTTCAGAAGGGGGGAATTGTTATTCATAGTAATTCGGTATTATCAGTAGTTATCAAATAAAAAACGGCTTTATTATCGTTGAAACGACTATACATTACAATTAGATAATTATTTTTTAAATTCGTTGACATCATCTGCTATTTTTGACACAGTTTATCGCTGAACTACCTTGAACGGTATTAGGTTAATATATTGt                                                                                                                                                                                                                <  1:302384/251‑1 (MQ=255)                             tattCCAAATGCTTTTTACAATGTATTCTTCTGCTAATGCCGCCGGGCTTTGTGCTTTCAGAAGGGGGGAATTGTTATTCATAGTAATTCGGTATTATCAGTAGTTATCAAATAAAAAACGGCTTTATTATCGTTGAAACGACTATACATTACAATTAGATAATTATTTTTTAAATTCGTTGACATCATCTGCTATTTTTGACACAGTTTATCGCTGAACTACCTTGAACGGTATTAGGTTAATATATTGt                                                                                                                                                                                                                <  1:420529/251‑1 (MQ=255)                                      tGCTTTTTACAATGTATTCTTCTGCTAATGCCGCCGGGCTTTGTGCTTTCAGAAGGGGGGAATTGTTATTCATAGTAATTCGGTATTATCAGTAGTTATCAAATAAAAAACGGCTTTATTATCGTTGAAACGACTATACATTACAATTAGATAATTATTTTTTAAATTCGTTGACATCATCTGCTATTTTTGACACAGTTTATCGCTGAACTACCTTGAACGGTATTAGGTTAatat                                                                                                                                                                                                                     >  1:640041/1‑237 (MQ=255)                                       gCTTTTTACAATGTATTCTTCTGCTAATGCCGCCGGGCTTTGTGCTTTCAGAAGGGGGGAATTGTTATTCATAGTAATTCGGTATTATCAGTAGTTATCAAATAAAAAACGGCTTTATTATCGTTGAAACGACTATACATTACAATTAGATAATTATTTTTTAAATTCGTTGACATCATCTGCTATTTTTGACACAGTTTATCGCTGAACTACCTTGAAc                                                                                                                                                                                                                                     <  1:758497/220‑1 (MQ=255)                                            ttACAATGTATTCTTCTGCTAATGCCGCCGGGCTTTGTGCTTTCAGAAGGGGGGAATTGTTATTCATAGTAATTCGGTATTATCAGTAGTTATCAAATAAAAAACGGCTTTATTATCGTTGAAACGACTATACATTACAATTAGATAATTATTTTTTAAATTCGTTGACATCATCTGCTATTTTTGACACAGTTTATCGCTGAACTACCTTGAACGGTATTAGGt                                                                                                                                                                                                                           >  1:159419/1‑225 (MQ=255)                                                 aTGTATTCTTCTGCTAATGCCGCCGGGCTTTGTGCTTTCAGAAGGGGGGAATTGTTATTCATAGTAATTCGGTATTATCAGTAGTTATCAAATAAAAAACGGCTTTATTATCGTTGAAACGACTATACATTACAATTAGATAATTATTTTTTAAATTCGTTGACATCATCTGCTATTTTTGACACAGTTTATCGCTGAACTACCTTGAACGGTATTAGGt                                                                                                                                                                                                                           <  1:142677/220‑1 (MQ=255)                                                   gTATTCTTCTGCTAATGCCGCCGGGCTTTGTGCTTTCAGAAGGGGGGAATTGTTATTCATAGTAATTCGGTATTATCAGTAGTTATCAAATAAAAAACGGCTTTATTATCGTTGAAACGACTATACATTACAATTAGATAATTATTTTTTAAATTCGTTGACATCATCTGCTATTTTTGACACAGTTTATCGCTGAACTACCTTGAACGGTATTAGGt                                                                                                                                                                                                                           >  1:503771/1‑218 (MQ=255)                                                            tGCTAATGCCGCCGGGCTTTGTGCTTTCAGAAGGGGGGAATTGTTATTCATAGTAATTCGGTATTATCAGTAGTTATCAAATAAAAAACGGCTTTATTATCGTTGAAACGACTATACATTACAATTAGATAATTATTTTTTAAATTCGTTGACATCATCTGCTATTTTTGACACAGTTTATCGCTGAACTACCTTGAACGGTATTAGGt                                                                                                                                                                                                                           >  1:89296/1‑209 (MQ=255)                                                                                     ttCAGAAGGGGGGAATTGTTATTCATAGTAATTCGGTATTATCAGTAGTTATCAAATAAAAAACGGCTTTATTATCGTTGAAACGACTATACATTACAATTAGATAATTATTTTTTAAATTCGTTGACATCATCTGCTATTTTTGACACAGTTTATCGCTGAACTACCTTGAACGGTATTAGGTTAATATATTGTTAATTAAGAAATTAAGGTCAACTTTATGGATAGTAATTATacac                                                                                                                                                                    <  1:765238/239‑1 (MQ=255)                                                                                         gAAGGGGGGAATTGTTATTCATAGTAATTCGGTATTATCAGTAGTTATCAAATAAAAAACGGCTTTATTATCGTTGAAACGACTATACATTACAATTAGATAATTATTTTTTAAATTCGTTGACATCATCTGCTATTTTTGACACAGTTTATCGCTGAACTACCTTGAACGGTATTAGGt                                                                                                                                                                                                                           <  1:606129/180‑1 (MQ=255)                                                                                              ggggAATTGTTATTCATAGTAATTCGGTATTATCAGTAGTTATCAAATAAAAAACGGCTTTATTATCGTTGAAACGACTATACATTACAATTAGATAATTATTTTTTAAATTCGTTGACATCATCTGCTATTTTTGACACAGTTTATCGCTGAACTACCTTGAACGGTATTAGGt                                                                                                                                                                                                                           <  1:695390/175‑1 (MQ=255)                                                                                              ggggAATTGTTATTCATAGTAATTCGGTATTATCAGTAGTTATCAAATAAAAAACGGCTTTATTATCGTTGAAACGACTATACATTACAATTAGATAATTATTTTTTAAATTCGTTGACATCATCTGCTATTTTTGACACAGTTTATCGCTGAACTACCTTGAACGGTATTAGGTTAatat                                                                                                                                                                                                                     <  1:509894/181‑1 (MQ=255)                                                                                                 gAATTGTTATTCATAGTAATTCGGTATTATCAGTAGTTATCAAATAAAAAACGGCTTTATTATCGTTGAAACGACTATACATTACAATTAGATAATTATTTTTTAAATTCGTTGACATCATCTGCTATTTTTGACACAGTTTATCGCTGAACTACCTTGAACGGTATTAGGTTAatat                                                                                                                                                                                                                     <  1:502459/178‑1 (MQ=255)                                                                                                      gTTATTCATAGTAATTCGGTATTATCAGTAGTTATCAAATAAAAAACGGCTTTATTATCGTTGAAACGACTATACATTACAATTAGATAATTATTTTTTAAATTCGTTGACATCATCTGCTATTTTTGACACAGTTTATCGCTGAACTACCTTGAACGGTATTAGGTTAATATATTGTTAATTAAGAAATTAAGGTCAACTTTATGGATAGTAATTATACACAGGCATTTTTTAAAAACTTTCTTGGtt                                                                                                                                         >  1:49410/1‑249 (MQ=255)                                                                                                      gTTATTCATAGTAATTCGGTATTATCAGTAGTTATCAAATAAAAAACGGCTTTATTATCGTTGAAACGACTATACATTACAATTAGATAATTATTTTTTAAATTCGTTGACATCATCTGCTATTTTTGACACAGTTTATCGCTGAACTACCTTGAACGGTATTAGGTTAATATATTGTTAATTAAGAAATTAAGGTCAACTTTATGGATAGTAATTATACACAGGCATTTTTTAAAAACTTTCTTGGtt                                                                                                                                         >  1:329643/1‑249 (MQ=255)                                                                                                                        gTATTATCAGTAGTTATCAAATAAAAAACGGCTTTATTATCGTTGAAACGACTATACATTACAATTAGATAATTATTTTTTAAATTCGTTGACATCATCTGCTATTTTTGACACAGTTTATCGCTGAACTACCTTGAACGGTATTAGGt                                                                                                                                                                                                                           >  1:64120/1‑149 (MQ=255)                                                                                                                          attatCAGTAGTTATCAAATAAAAAACGGCTTTATTATCGTTGAAACGACTATACATTACAATTAGATAATTATTTTTTAAATTCGTTGACATCATCTGCTATTTTTGACACAGTTTATCGCTGAACTACCTTGAACGGTATTAGGTTAATATATTGTTAATTAAGAAATTAAGGTCAACTTTATGGATAGTAATTATACACAGGCATTTTTTAAAAACTTTCTTGGTTCTAGCCCGGGCTGGTATAAAAt                                                                                                                   <  1:621743/251‑1 (MQ=255)                                                                                                                                   agtTATCAAATAAAAAACGGCTTTATTATCGTTGAAACGACTATACATTACAATTAGATAATTATTTTTTAAATTCGTTGACATCATCTGCTATTTTTGACACAGTTTATCGCTGAACTACCTTGAACGGTATTAGGTTAATATATTGTTAATTAAGAAATTAAGGTCAACTTTATGGATAGTAATTATacac                                                                                                                                                                    <  1:488684/193‑1 (MQ=255)                                                                                                                                            atgaaaaaCGGCTTTATTATCGTTGAAACGACTATACATTACAATTAGATAATTATTTTTTAAATTCGTTGACATCATCTGCTATTTTTGACACAGTTTATCGCTGAACTACCTTGAACGGTATTAGGt                                                                                                                                                                                                                           <  1:344082/126‑1 (MQ=255)                                                                                                                                                      gCTTTATTATCGTTGAAACGACTATACATTACAATTAGATAATTATTTTTTAAATTCGTTGACATCATCTGCTATTTTTGACACAGTTTATCGCTGAACTACCTTGAACGGTATTAgg                                                                                                                                                                                                                            >  1:525151/1‑118 (MQ=255)                                                                                                                                                                      aaCGACTATACATTACAATTAGATAATTATTTTTTAAATTCGTTGACATCATCTGCTATTTTTGACACAGTTTATCGCTGAACTACCTTGAACGGTATTAGGTTAATATATTGTTAATTAAGAAATTAAGGTCAACTTTATGGATAGTAATTATacac                                                                                                                                                                    >  1:648248/1‑158 (MQ=255)                                                                                                                                                                        cGACTATACATTACAATTAGATAATTATTTTTTAAATTCGTTGACATCATCTGCTATTTTTGACACAGTTTATCGCTGAACTACCTTGAACGGTATTAGGt                                                                                                                                                                                                                           <  1:760800/101‑1 (MQ=255)                                                                                                                                                                        cGACTATACATTACAATTAGATAATTATTTTTTAAATTCGTTGACATCATCTGCTATTTTTGACACAGTTTATCGCTGAACTACCTTGAACGGTATTAGGTTAATATATTGTTAATTAAGAAATTAAGGTCAACTTTATGGATAGTAATTATac                                                                                                                                                                      <  1:10159/154‑1 (MQ=255)                                                                                                                                                                         gACTATACATTACAATTAGATAATTATTTTTTAAATTCGTTGACATCATCTGCTATTTTTGACACAGTTTAtcgc                                                                                                                                                                                                                                                    <  1:348561/75‑1 (MQ=255)                                                                                                                                                                             ataCATTACAATTAGATAATTATTTTTTAAATTCGTTGACATCATCTGCTATTTTTGACACAGTTTATCGCTGAACTACCTTGAACGGTATTAGGt                                                                                                                                                                                                                           <  1:175687/96‑1 (MQ=255)                                                                                                                                                                             ataCATTACAATTAGATAATTATTTTTTAAATTCGTTGACATCATCTGCTATTTTTGACACAGTTTATCGCTGAACTACCTTGAACGGTATTAGGt                                                                                                                                                                                                                           >  1:780656/1‑96 (MQ=255)                                                                                                                                                                               aCATTACAATTAGATAATTATTTTTTAAATTCGTTGACATCATCTGCTATTTTTGACACAGTTTATCGCTGAACTACCTTGAACGGTATTAGGTTAATATATTGTTAATTAAGAAATTAAGGTCAACTTTATGGATAGTAATTATACACAGGCATTTTTTAAAAACTTTCTTGGTTCTAGCCCGGGCTGGTATAAAATAGCGATCGTCGCATTTCTTGTCATTAATCCCGTCTTATTTGCTATTTCACCtt                                                              >  1:276056/1‑251 (MQ=255)                                                                                                                                                                                cATTACAATTAGATAATTATTTTTTAAATTCGTTGACATCATCTGCTATTTTTGACACAGTTTATCGCTGAACTACCTTGAACGGTATTAGGt                                                                                                                                                                                                                           <  1:251219/93‑1 (MQ=255)                                                                                                                                                                                                          aaTTCGTTGACATCATCTGCTATTTTTGACACAGTTTATCGCTGAACTACCTTGAACGGTATTAGGTTAATATATTGTTAATTAAGAAATTAAGGTCAACTTTATGGATAGTAATTATACACAGGCATTTTTTAAAAACTTTCTTGGTTCTAGCCCGGGCTGGTATAAAATAGCGATCGTCGCATTTCTTGTCATTAATCCCGTCTTATTTGCTATTTCACCTTTTTGGGCGGGGTGGTTATTAGTCATTg                                   <  1:73050/251‑1 (MQ=255)                                                                                                                                                                                                             tCGTTGACATCATCTGCTATTTTTGACACAGTTTATCGCTGAACTACCTTGAACGGTATTAGGTTAatat                                                                                                                                                                                                                     <  1:657525/70‑1 (MQ=255)                                                                                                                                                                                                             tCGTTGACATCATCTGCTATTTTTGACACAGTTTATCGCTGAACTACCTTGAACGGTATTAGGTTAATATATTGTTAATTAAGAAATTAAGGTCAACTTTATGGATAGTAATTATACACAGGCATTTTTTAAAAACTTTCTTGGTTCTAGCCCGGGCTGGTATAAAATAGCGATCGTCGCATTTCTTGTCATTAATCCCGTCTTATTTGCTATTTCACCTTTTTGGGCGGGGTGGTTATTAGTCATTGAAt                                >  1:169858/1‑251 (MQ=255)                                                                                                                                                                                                                       catcTGCTATTTTTGACACAGTTTATCGCTGAACTACCTTGAACGGTATTAGGTTAATATATTGTTAATTAAGAAATTAAGGTCAACTTTATGGATAGTAATTATACACAGGCATTTTTTAAAAACTTTCTTGGTTCTAGCCCGGGCTGGTATAAAATAGCGATCGTCGCATTTCTTGTCATTAATCCCGTCTTATTTGCTATTTCACCTTTTTGGGCGGGGTGGTTATTAGt                                        <  1:698573/233‑1 (MQ=255)                                                                                                                                                                                                                                           gTTTATCGCTGAACTACCTTGAACGGTATTAGGTTAATATATTGTTAATTAAGAAATTAAGGTCAACTTTATGGATAGTAATTATACACAGGCATTTTTTAAAAACTTTCTTGGTTCTAGCCCGGGCTGGTATAAAATAGCGATCGTCGCATTTCTTGTCATTAATCCCATCTTATTTGCTATTTCACCTTTTTGGGCGGGGTGGTTATTAGTCATTGAATTCATTTTCACCCTAGCTATGGCGCTAAAAt  >  1:434340/1‑251 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                      GGTAAATCCGAACCGGGTGGGAAATGATTATTCCAAATGCTTTTTACAATGTATTCTTCTGCTAATGCCGCCGGGCTTTGTGCTTTCAGAAGGGGGGAATTGTTATTCATAGTAATTCGGTATTATCAGTAGTTATCAAATAAAAAACGGCTTTATTATCGTTGAAACGACTATACATTACAATTAGATAATTATTTTTTAAATTCGTTGACATCATCTGCTATTTTTGACACAGTTTAGCGCTGAACTACCTTGAACGGTATTAGGTTAATATATTGTTAATTAAGAAATTAAGGTCAACTTTATGGATAGTAATTATACACAGGCATTTTTTAAAAACTTTCTTGGTTCTAGCCCGGGCTGGTATAAAATAGCGATCGTCGCATTTCTTGTCATTAATCCCGTCTTATTTGCTATTTCACCTTTTTGGGCGGGGTGGTTATTAGTCATTGAATTCATTTTCACCCTAGCTATGGCGCTAAAAT  >  1/190724‑191208

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑