Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,420,483	C→T	L17L (TTG→TTA)	unknown ←	Recombination protein RecR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,420,483	0	C	T	100.0%	188.6 / NA	71	L17L (TTG→TTA)	unknown	Recombination protein RecR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (30/41);  total (30/41)

CTGTTTGCTCGATAGCAAGAATATCCGCCGGCGTTTCCACCACGCAAAGCAAGCCCGAATTTTGGCGGCGTGGATTACTGCAAATGTTGCACACGTCTTCTTCCGTAAAAGTGCGGCAATGTTCGCAATGCCCGATTTTCGACATCGCTTCCGTCAGCCCTTGCGCCAAGCGCATTCCGCCGCTGCGATTGCGTTGCAATAAATGATACGCCATACGTTGCGCGGATTTCGGACCGACGCCCGGCAATACGCGTAAACTTTCGATCAGATTTTCTAACAAGGGGCTGGTTTGCATAAGGTTCCCGATGGATTTGCTGAAAAAATAAACCGCACTTTGCCGTTAATAAAGAAAAAGTGCGGTCGTTTTTTAGGACGTTTTTTAAAACGGCATTTTGAAGCCCGGCGGTAAAGACATACCGGCGGTGACTGACGCCATTTTTTCTTTTTGTAACTCTTCGGCACGACGCACTGCATCGT  >  1/1420239‑1420715                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                         cTGTTTGCTCGATAGCAAGAATATCCGCCGGCATTTCCACCACGCAAAGCAAGCCCGAATTTTGGCGGCGTGGATTACTGCAAATGTTGCACACGTCTTCTTCCGTAAAAGTGCGGCAATGTTCGCAATGCCCGATTTTCGACATCGCTTCCGTCAGCCCTTGCGCCAAGCGCATTCCGCCGCTGCGATTGCGTTGCAATAAATGATACGCCATACGTTGCGCGGATTTCGGACCGACGCCCGGTAATAcg                                                                                                                                                                                                                                    <  1:856655/251‑1 (MQ=255) cTGTTTGCTCGATAGCAAGAATATCCGCCGGCATTTCCACCACGCAAAGCAAGCCCGAATTTTGGCGGCGTGGATTACTGCAAATGTTGCACACGTCTTCTTCCGTAAAAGAGCGGCAATGTTCGCAATGCCCGATTTTCGACATCGCTTCCGTCAGCCCTTGCGCCAAGCGCATTCCGCCGCTGCGATTGCGTTGCAATAAATGATACGCCATACGTTGCGCGGATTTCGGACCGACGCCCGGTAATAcg                                                                                                                                                                                                                                    <  1:191895/251‑1 (MQ=255)  tGTTTGCTCGATAGCAAGAATATCCGCCGGCATTTCCACCACGCAAAGCAAGCCCGAATTTTGGCGGCGTGGATTACTGCAAATGTTGCACACGTCTTCTTCCGTAAAAGTGCGGCAATGTTCGCAATGCCCGATTTTCGACATCGCTTCCGTCAGCCCTTGCGCCAAGCGCATTCCGCCGCTGCGATTGCGTTGCAATAAATGATACGCCATACGTTGCGCGGATTTCGGACCGACGCCCGGTAATAcgc                                                                                                                                                                                                                                   <  1:536647/251‑1 (MQ=255)    tttGCTCGATAGCAAGAATATCCGCCGGCATTTCCACCACGCAAAGCAAGCCCGAATTTTGGCGGCGTGGATTACTGCAAATGTTGCACACGTCTTCTTCCGTAAAAGTGCGGCAATGTTCGCAATGCCCGATTTTCGACATCGCTTCCGTCAGCCCTTGCGCCAAGCGCATTCCGCCGCTGCGATTGCGTTGCAATAAATGATACGCCATACGTTGCGCGGATTTCGGACCGACGCCCGGTAATAcgcg                                                                                                                                                                                                                                  <  1:756339/250‑1 (MQ=255)                          cgccgGCATTTCCACCACGCAAAGCAAGCCCGAATTTTGGCGGCGTGGATTACTGCAAATGTTGCACACGTCTTCTTCCGTAAAAGTGCGGCAATGTTCGCAATGCCCGATTTTCGACATCGCTTCCGTCAGCCCTTGCGCCAAGCGCATTCCGCCGCTGCGATTGCGTTGCAATAAATGATACGCCATACGTTGCGCGGATTTCGGACCGACGCCCGGTAATACGCGTAAACTTTCGATCAGATTTTCTa                                                                                                                                                                                                           <  1:402692/251‑1 (MQ=255)                          cgccgGCATTTCCACCACGCAAAGCAAGCCCGAATTTTGGCGGCGTGGATTACTGCAAATGTTGCACACGTCTTCTTCCGTAAAAGTGCGGCAATGTTCGCAATGCCCGATTTTCGACATCGCTTCCGTCAGCCCTTGCGCCAAGCGCATTCCGCCGCTGCGATTGCGTTGCAATAAATGATACGCCATACGTTGCGCGGATTTCGGACCGACGCCCGGTAATACGCGTAAACTTTCGATCAGATTTTCTa                                                                                                                                                                                                           <  1:247903/251‑1 (MQ=255)                               gcatttCCACCACGCAAAGCAAGCCCGAATTTTGGCGGCGTGGATTACTGCAAATGTTGCACACGTCTTCTTCCGTAAAAGTGCGGCAATGTTCGCAATGCCCGATTTTCGACATCGCTTCCGTCAGCCCTTGCGCCAAGCGCATTCCGCCGCTGCGATTGCGTTGCAATAAATGATACGCCATACGTTGCGCGGATTTCGGACCGACGCCCGGTAATACGCGTAAACTTTCGATCAGATTTTCTAACAAg                                                                                                                                                                                                      >  1:761598/4‑251 (MQ=255)                                        ccacGCAAAGCAAGCCCGAATTTTGGCGGCGTGGATTACTGCAAATGTTGCACACGTCTTCTTCCGTAAAAGTGCGGCAATGTTCGCAATGCCCGATTTTCGACATCGCTTCCGTCAGCCCTTGCGCCAAGCGCATTCCGCCGCTGCGATTGCGTTGCAATAAATGATACGCCATACGTTGCGCGGATTTCGGACCGACGCCCGGTAATACGCGTAAACTTTCGATCAGATTTTCTAACAAGGGGCTGGtt                                                                                                                                                                                             <  1:78615/251‑1 (MQ=255)                                          acGCAAAGCAAGCCCGAATTTTGGCGGCGTGGATTACTGCAAATGTTGCACACGTCTTCTTCCGTAAAAGTGCGGCAATGTTCGCAATGCCCGATTTTCGACATCGCTTCCGTCAGCCCTTGCGCCAAGCGCATTCCGCCGCTGCGATTGCGTTGCAATAAATGATACGCCATACGTTGCGCGGATTTCGGACCGACGCCCGGTAATACGCGTAAACTTTCGATCAGATTTTCTAACAAGGGGc                                                                                                                                                                                                  >  1:824091/1‑244 (MQ=255)                                          acGCAAAGCAAGCCCGAATTTTGGCGGCGTGGATTACTGCAAATGTTGCACACGTCTTCTTCCGTAAAAGTGCGGCAATGTTCGCAATGCCCGATTTTCGACATCGCTTCCGTCAGCCCTTGCGCCAAGCGCATTCCGCCGCTGCGATTGCGTTGCAATAAATGATACGCCATACGTTGCGCGGATTTCGGACCGACGCCCGGTAATACGCGTAAACTTTCGATCAGATTTTCTAACAAGGGGCTGGTTTg                                                                                                                                                                                           >  1:546119/1‑251 (MQ=255)                                          acGCAAAGCAAGCCCGAATTTTGGCGGCGTGGATTACTGCAAATGTTGCACACGTCTTCTTCCGTAAAAGTGCGGCAATGTTCGCAATGCCCGATTTTCGACATCGCTTCCGTCAGCCCTTGCGCCAAGCGCATTCCGCCGCTGCGATTGCGTTGCAATAAATGATACGCCATACGTTGCGCGGATTTCGGACCGACGCCCGGTAATACGCGTAAACTTTCGATCAGATTTTCTAACAAGGGGCTGGTTTg                                                                                                                                                                                           >  1:854626/1‑251 (MQ=255)                                           cGCAAAGCAAGCCCGAATTTTGGCGGCGTGGATTACTGCAAATGTTGCACACGTCTTCTTCCGTAAAAGTGCGGCAATGTTCGCAATGCCCGATTTTCGACATCGCTTCCGTCAGCCCTTGCGCCAAGCGCATTCCGCCGCTGCGATTGCGTTGCAATAAATGATACGCCATACGTTGCGCGGATTTCGGACCGACGCCCGGTAATACGCGTAAACTTTCGATc                                                                                                                                                                                                                     >  1:297360/1‑224 (MQ=255)                                             cAAAGCAAGCCCGAATTTTGGCGGCGTGGATTACTGCAAATGTTGCACACGTCTTCTTCCGTAAAAGTGCGGCAATGTTCGTAATGCCCGATTTTCGACATCGCTTCCGTCAGCCCTTGCGCCAAGCGCATTCCGCCGCTGCGATTGCGTTGCAATAAATGATACGCCATACGTTGCGCGGATTTCGGACCGACGCCCGGTAATACGCGTAAACTTTCGATCAGATTTTCTAACAAGGGGCTGGTTTGCAt                                                                                                                                                                                        <  1:450957/251‑1 (MQ=255)                                                  caagcCCGAATTTTGGCGGCGTGGATTACTGCAAATGTTGCACACGTCTTCTTCCGTAAAAGTGCGGCAATGTTCGCAATGCCCGATTTTCGACATCGCTTCCGTCAGCCCTTGCGCCAAGCGCATTCCGCCGCTGCGATTGCGTTGCAATAAATGATACGCCATACGTTGCGCGGATTTCGGACCGACGCCCGGTAATACGCGTAAACTTTCGATCAGATTTTCTAACAAGGGGCTGGTTTGCATAAgg                                                                                                                                                                                    <  1:66039/250‑1 (MQ=255)                                                  caagcCCGAATTTTGGCGGCGTGGATTACTGCAAATGTTGCACACGTCTTCTTCCGTAAAAGTGCGGCAATGTTCGCAATGCCCGATTTTCGACATCGCTTCCGTCAGCCCTTGCGCCAAGCGCATTCCGCCGCTGCGATTGCGTTGCAATAAATGATACGCCATACGTTGCGCGGATTTCGGACCGACGCCCGGTAATACGCGTAAACTTTCGATCAGATTTTCTAACAAGGGGCTGGTTTGCATAAGGt                                                                                                                                                                                   <  1:830667/251‑1 (MQ=255)                                                      cccGAATTTTGGCGGCGTGGATTACTGCAAATGTTGCACACGTCTTCTTCCGTAAAAGTGCGGCAATGTTCGCAATGCCCGATTTTCGACATCGCTTCCGTCAGCCCTTGCGCCAAGCGCATTCCGCCGCTGCGATTGCGTTGCAATAAATGATACGCCATACGTTGCGCGGATTTCGGACCGACGCCCGGTAATACGCGTAAACTTTCGATCAGATTTTCTAACAAGGGGCTGGTTTGCATAAGGTTcc                                                                                                                                                                                <  1:609891/250‑1 (MQ=255)                                                          aaTTTTGGCGGCGTGGATTACTGCAAATGTTGCACACGTCTTCTTCCGTAAAAGTGCGGCAATGTTCGCAATGCCCGATTTTCGACATCGCTTCCGTCAGCCCTTGCGCCAAGCGCATTCCGCCGCTGCGATTGCGTTGCAATAAATGATACGCCATACGTTGCGCGGATTTCGGACCGACGCCCGGTAATACGCGTAAACTTTCGATCAGATTTTCTAACAAGGGGCTGGtt                                                                                                                                                                                             >  1:602148/1‑233 (MQ=255)                                                          aaTTTTGGCGGCGTGGATTACTGCAAATGTTGCACACGTCTTCTTCCGTAAAAGTGCGGCAATGTTCGCAATGCCCGATTTTCGACATCGCTTCCGTCAGCCCTTGCGCCAAGCGCATTCCGCCGCTGCGATTGCGTTGCAATAAATGATACGCCATACGTTGCGCGGATTTCGGACCGACGCCCGGTAATACGCGTAAACTTTCGATCAGATTTTCTAACAAGGGGCTGGTTTGCATAAGGTTCCCg                                                                                                                                                                              <  1:17936/248‑1 (MQ=255)                                                                      gTGGATTACTGCAAATGTTGCACACGTCTTCTTCCGTAAAAGTGCGGCAATGTTCGCAATGCCCGATTTTCGACATCGCTTCCGTCAGCCCTTGCGCCAAGCGCATTCCGCCGCTGCGATTGCGTTGCAATAAATGATACGCTATACGTTGCGCGGATTTCGGACCGACGCCCGGTAATACGCGTAAACTTTCGATCAGATTTTCTAACAAGGGGCTGGTTTGCATAAGGTTCCCGATGGATTTGCTGaaa                                                                                                                                                               >  1:29269/1‑251 (MQ=255)                                                                      gTGGATTACTGCAAATGTTGCACACGTCTTCTTCCGTAAAAGTGCGGCAATGTTCGCAATGCCCGATTTTCGACATCGCTTCCGTCAGCCCTTGCGCCAAGCGCATTCCGCCGCTGCGATTGCGTTGCAATAAATGATACGCCATACGTTGCGCGGATTTCGGACCGACACCCGGTAATACGCGTAAACTTTCGATCAGATTTTCTAACAAGGGGCTGGTTTGCATAAGGTTCCCGATGGATTTGCTGaaa                                                                                                                                                               >  1:431030/1‑251 (MQ=255)                                                                         gATTACTGCAAATGTTGCACACGTCTTCTTCCGTAAAAGTGCGGCAATGTTCGCAATGCCCGATTTTCGACATCGCTTCCGTCAGCCCTTGCGCCAAGCGCATTCCGCCGCTGCGATTGCGTTGCAATAAATGATACGCCATACGTTGCGCGGATTTCGGACCGACGCCCGGTAATACGCGTAAACTTTCGATCAGATTTTCTAACAAGGGGCTGGTTTGCATAAGGTTCCCGATGGATTTGCTGaaaaaa                                                                                                                                                            >  1:361194/1‑251 (MQ=255)                                                                          aTTACTGCAAGTGTTGCACACGTCTTCTTCCGTAAAAGTGCGGCAATGTTCGCAATGCCCGATTTTCGACATCGCTTCCGTTAGCCCTTGCGCCAAGCGCATTCCGCCGCTGCGATTGCGTTGCAATAAATGATACGCCATACGTTGCGCGGATTTCGGACCGACGCCCGGTAATACGCGTAAACTTTCGATCAGATTTTCTAACAAGGGGCTGGTTTGCATAAGGTTCCCGAGGGATTTGCTGAAAAAAt                                                                                                                                                           <  1:425725/251‑1 (MQ=255)                                                                              cTGCAAATGTTGCACACGTCTTCTTCCGTAAAAGTGCGGCAATGTTCGCAATGCCCGATTTTCGACATCGCTTCCGTCAGCCCTTGCGCCAAGCGCATTCCGCCGCTGCGATTGCGTTGCAATAAATGATACGCCATACGTTGCGCGGATTTCGGACCGACGCCCGGTAATACGCGTAAAc                                                                                                                                                                                                                             <  1:384579/181‑1 (MQ=255)                                                                              cTGCAAATGTTGCACACGTCTTCTTCCGTAAAAGTGCGGCAATGTTCGCAATGCCCGATTTTCGACATCGCTTCCGTCAGCCCTTGCGCCAAGCGCATTCCGCCGCTGCGATTGCGTTGCAATAAATGATACGCCATACGTTGCGCGGATTTCGGACCGACGCCCGGTAATACGCGTAAACTTTCGATCAGATTTTCTaa                                                                                                                                                                                                          >  1:478944/1‑200 (MQ=255)                                                                               tGCAAATGTTGCACACGTCTTCTTCCGTAAAAGTGCGGCAATGTTCGCAATGCCCGATTTTCGACATCGCTTCCGTCAGCCCTTGCGCCAAGCGCATTCCGCCGCTGCGATTGCGTTGCAATAAATGATACGCCATACGTTGCGCGGATTTCGGACCGACGCCCGGTAATACGCGTAAACTTTCGATCAGATTTTCTAACAAGGGGCTGGTTTGCATAAGGTTCCCGATGGATTTGCTGAAAAAATAAAcc                                                                                                                                                      <  1:548301/251‑1 (MQ=255)                                                                                       ttGCACACGTCTTCTTCCGTAAAAGTGCGGCAATGTTCGCAATGCCCGATTTTCGACATCGCTTCCGTCAGCCCTTGCGCCAAGCGCATTCCGCCGCTGCGATTGCGTTGCAATAAATGATACGCCATACGTTGCGCGGATTTCGGACCGACGCCCGGTAATACGCGTAAACTTTCGATCAGATTTTCTAACAAGGGGCTGGTTTGCATAAGGTTCCCGATGGATTTGCTGAAAAAATAAACCGCACTTTg                                                                                                                                              >  1:77500/1‑251 (MQ=255)                                                                                        tGCACACGTCTTCTTCCGTAAAAGTGCGGCAATGTTCGCAATGCCCGGTTTTCGACATCGCTTCCGTCAGCCCTTGCGCCAAGCGCATTCCGCCGCTGCGATTGCGTTGCAATAAATGATACGCCATACGTTGCGCGGATTTCGGACCGACGCCCGGTAATACGCGTAAACTTTCGATCAGATTTTCTAACAAGGGGCTGGTTTGCATAAGGTTCCCGATGGATTTGCTGAAAAAATAAACCGCACTTTGc                                                                                                                                             <  1:404987/251‑1 (MQ=255)                                                                                          cacaCGTCTTCTTCCGTAAAAGTGCGGCAATGTACGCAATGCCCGATTTTCGACATCGCTTCCGTCAGCCCTTGCGCCAAGCGCATTCCGCCGCTGCGATTGCGTTGCAATAAATGATACGCCATACGTTGCGCGGATTTCGGACCGACGCCCGGTAATACGCGTAAACTTTCGATCAGATTTTCTAACAAGGGGCTGGTTTGCATAAGGTTCCCGATGGATTTGCTGAAAAAATAAACCGCACTTTGCCg                                                                                                                                           <  1:296997/251‑1 (MQ=255)                                                                                              cGTCTTCTTCCGTAAAAGTGCGGCAATGTTCGCAATGCCCGATTTTCGACATCGCTTCCGTCAGCCCTTGCGCCAAGCGCATTCCGCCGCTGCGATTGCGTTGCAATAAATGATACGCCATACGTTGCGCGGATTTCGGACCGACGCCCGGTAATACGCGTAAACTTTCGATc                                                                                                                                                                                                                     <  1:14431/173‑1 (MQ=255)                                                                                              cGTCTTCTTCCGTAAAAGTGCGGCAATGTTCGCAATGCCCGATTTTCGACATCGCTTCCGTCAGCCCTTGCGCCAAGCGCATTCCGCCGCTGCGATTGCGTTGCAATAAATGATACGCCATACGTTGCGCGGATTTCGGACCGACGCCCGGTAATACGCGTAAACTTTCGATCAGATTTTCTAACAAGGGGCTGGTTTGCATAAGGTTCCCGATGGATTTGCTGAAAAAATAAAc                                                                                                                                                       <  1:374048/235‑1 (MQ=255)                                                                                               gTCTTCTTCCGTAAAAGTGCGGCAATGTTCGCAATGCCCGATTTTCGACATCGCTTCCGTCAGCCCTTGCGCCAAGCGCATTCCGCCGCTGCGATTGCGTTGCAATAAATGATACGCCATACGTTGCGCGGATTTCGGACCGCCGCCCGGTAATACGCGTAAACTTTCGATCAGATTTTCTAACAAGGGGCTGGtt                                                                                                                                                                                             >  1:126429/1‑196 (MQ=255)                                                                                                tcttctTCCGTAAAAGTGCGGCAATGTTCGCAATGCCCGATTTTCGACATCGCTTCCGTCAGCCCTTGCGCCAAGCGCATTCCGCCGCTGCGATTGCGTTGCAATAAATGATACGCCATACGTTGCGCGGATTTCGGACCGACGCCCGGTAATACGCGTAAACTTTCGATCAGATTTTCTAACAAGGGGCTGGTTTGCATAAGGTTCCCGATGGATTTGCTGAAAAAATAAACCGCACTTTGCCGTtaat                                                                                                                                      <  1:265234/250‑1 (MQ=255)                                                                                                       ccGTAAAAGTGCGGCAATGTTCGCAATGCCCGATTTTCGACATCGCTTCCGTCAGCCCTTGCGCCAAGCGCATTCCGCCGCTGCGATTGCGTTGCAATAAATGATACGCCATACGTTGCGCGGATTTCGGACCGACGCCCGGTAATACGCGTAAACTTTCGATCAGATTTTCTAACAAGGGGCTGGTTTGCATAAGGTTCCCGATGGATTTGCTGAAAAAATAAACCGCACTTTGCCGTTAATGAAGaaaa                                                                                                                              >  1:708875/1‑251 (MQ=255)                                                                                                          tAAAAGTGCGGCAATGTTCGCAATGCCCGATTTTCGACATCGCTTCCGTCAGCCCTTGCGCCAAGCGCATTCCGCCGCTGCGATTGCGTTGCAATAAATGATACGCCATACGTTGCGCGGATTTCGGACCGACGCCCGGTAATACGCGTAAACTTTCGATCAGATTTTCTAACAAGGGGCTGGTTTGCATAAGGTTCCCGATGGATTTGCTGAAAAAATAAACCGCACTTTGCCGTTAATGAAGAAAAAg                                                                                                                            <  1:308542/250‑1 (MQ=255)                                                                                                          tAAAAGTGCGGCAATGTTCGCAATGCCCGATTTTCGACATCGCTTCCGTCAGCCCTTGCGCCAAGCGCATTCCGCCGCTGCGATTGCGTTGCAATAAATGATACGCCATACGTTGCGCGGATTTCGGACCGACGCCCGGTAATACGCGTAAACTTTCGATCAGATTTTCTAACAAGGGGCTGGTTTGCATAAGGTTCCCGATGGATTTGCTGAAAAAATAAACCGCACTTTGCCGTTAATGAAGAAAAAg                                                                                                                            <  1:776279/250‑1 (MQ=255)                                                                                                                   ggCAATGTTCGCAATGCCCGATTTTCGACATCGCTTCCGTCAGCCCTTGCGCCAAGCGCATTCCGCCGCTGCGATTGCGTTGCAATAAATGATACGCCATACGTTGCGCGGATTTCGGACCGACGCCCGGTAATACGCGTAAACTTTCGATCAGATTTTCTAACAAGGGGCTGGTTTGCATAAGGTTCCCGATGGATTTGCTGAAAAAATAAACCGCACTTTGCCGTTAATGAAGAAAAAGTGCGGTCGtt                                                                                                                  >  1:343409/1‑251 (MQ=255)                                                                                                                        tGTTCGCAATGCCCGATTTTCGACATCGCTTCCGTCAGCCCTTGCGCCAAGCGCATTCCGCCGCTGCGATTGCGTTGCAATAAATGATACGCCATACGTTGCGCGGATTTCGGACCGACGCCCGGTAATACGCGTAAACTTTCGATCAGATTTTCTAACAAGGGGCTGGTTTGCATAAGGt                                                                                                                                                                                   >  1:320784/1‑181 (MQ=255)                                                                                                                                       aTTTTCGACATCGCTTCCGTCAGCCCTTGCGCCAAGCGCATTCCGCCGCTGCGATTGCGTTGCAATAAATGATACGCCATACGTTGCGCGGATTTCGGACTGACGCCCGGTAATACGCGTAAACTTTCGATCAGATTTTCTAACAACGGGCTGGTTTGCTTAAGGCTCCCGATGGGTTTGCTGAAAAAATAAACCCCACTTTCCCGTTAATGAAGAAAAAGTGCGGTCGTTTTTTAGGACGTTTTTTaaaa                                                                                              >  1:218140/1‑251 (MQ=255)                                                                                                                                                 tCGCTTCCGTCAGCCCTTGCGCCAAGCGCATTCCGCCGCTGCGATTGCGTTGCAATAAATGATACGCCATACGTTGCGCGGATTTCGGACCGACGCCCGGTAATACGCGTAAACTTTCGATCAGATTTTCTAACAAGGGGCTGGTTTGCATAAGGt                                                                                                                                                                                   >  1:110575/1‑156 (MQ=255)                                                                                                                                                 tCGCTTCCGTCAGCCCTTGCGCCAAGCGCATTCCGCCGCTGCGATTGCGTTGCAATAAATGATACGCCATACGTTGCGCGGATTTCGGACCGACGCCCGGTAATACGCGTAAACTTTCGATCAGATTTTCTAACAAGGGGCTGGTTTGCATAAGGTTCCCGATGGATTTGCTGAAAAAATAAACCGCACTTTGCCGTTAATGAAGAAAAAGTGCGGTCGTTTTTTAGGACGTTTTTTAAAACGGCATTTTg                                                                                    <  1:104655/251‑1 (MQ=255)                                                                                                                                                  cGCTTCCGTCAGCCCTTGCGCCAAGCGCATTCCGCCGCTGCGATTGCGTTGCAATAAATGATACGCCATACGTTGCGCGGATTTCGGACCGACGCCCGGTAATACGCGTAAACTTTCGATCAGATTTTCTAACAAGGGGCTGGTTTGCATAAGGTTCCCGATGGATTTGCTGAAAAAATAAACCGCACTTTGCCGTTAATGAAGAAAAAGTGCGGTCGTTTTTTAGGACGTTTTTTAAAACGGCATTTTGa                                                                                   <  1:152332/251‑1 (MQ=255)                                                                                                                                                                   gcgcCAAGCGCATTCCGCCGCTGCGATTGCGTTGCAATAAATGATACGCCATACGTTGCGCGGATTTCGGACCGACGCCCGGTAATACGCGTAAACTTTCGATCAGATTTTCTAACAAGGGGCTGGTTTGCATAAGGTTCCCGATGGATTTGCTGAAAAAATAAACCGCACTTTGCCGTTAATGAAGAAAAAGTGCGGTCGTTTTTTAGGACGTTTTTTAAAACGGCATTTTGAAGCCCGGCGGTAAAGa                                                                   >  1:324016/1‑250 (MQ=255)                                                                                                                                                                    cgcCAAGCGCATTCCGCCGCTGCGATTGCGTTGCAATAAATGATACGCCATACGTTGCGCGGATTTCGGACCGACGCCCGGTAATACGCGTAAACTTTCGATCAGATTTTCTAACAAGGGGCTGGTTTGCATAAGGTTCCCGGTGGATTTGCTGAAAAAATAAACCGCACTTTGCCGTTAATGAAGAAAAAGTGCGGTCGTTTTTTAGGACGTTTTTTAAAACGGCATTTTGAAGCCCGGCGGTAAAGAc                                                                  <  1:572162/250‑1 (MQ=255)                                                                                                                                                                    cgcCAAGCGCATTCCGCCGCTGCGATTGCGTTGCAATAAATGATACGCCATACGTTGCGCGGATTTCGGACCGACGCCCGGTAATACGCGTAAACTTTCGATCAGATTTTCTAACAAGGGGCTGGTTTGCATAAGGTTCCCGATGGATTTGCTGAAAAAATAAACCGCACTTTGCCGTTAATGAAGAAAAAGTGCGGTCGTTTTTTAGGACGTTTTTTAAAACGGCATTTTGAAGCCCGGCGGTAAAGAc                                                                  <  1:750276/250‑1 (MQ=255)                                                                                                                                                                      ccAAGCGCATTACGCCGCTGCGATTGCGTTGCAATAAATGATACGCCATACGTTGCGCGGATTTCGGACCGACGCCCGGTAATACGCGTAAACTTTCGATCAGATTTTCTAACAAGGGGCTGGTTTGCATAAGGTTCCCGATGGATTTGCTGAAAAAATAAACCGCACTTTGCCGTTAATGAAGAAAAAGTGCGGTCGTTTTTTAGGACGTTTTTTAAAACGGCATTTTGAAGCCCGGCGGTAAAGACAt                                                                <  1:121912/250‑1 (MQ=255)                                                                                                                                                                          gcgcATTCCGCCGCTGCGATTGCGTTGCAATAAATGATACGCCATACGTTGCGCGGATTTCGGACCGACGCCCGGTAATACGCGTAAACTTTCGATCAGATTTTCTAACAAGGGGCTGGTTTGCATAAGGTTCCCGATGGATTTGCTGAAAAAATAAACCGCACTTTGCCGTTAATGAAGAAAAAGTGCGGTCGTTTTTTAGGACGTTTTTTAAAACGGCATTTTGAAGCCCGGCGGTAAAGACATACcgg                                                           >  1:284314/1‑251 (MQ=255)                                                                                                                                                                                 ccgccgCTGCGATTGCGTTGCAATAAATGATACGCCATACGTTGCGCGGATTTCGGACCGACGCCCGGTAATACGCGTAAACTTTCGATCAGATTTTCTAACAAGGGGCTGGTTTGCATAAGGTTCCCGATGGATTTGCTGAAAAAATAAACCGCACTTTGCCGTTAATGAAGAAAAAGTGCGGTCGTTTTTTAGGACGTTTTTTAAAACGGCATTTTGAAGCCCGGCGGTAAAGACATACCGGCGGtgac                                                    <  1:167537/251‑1 (MQ=255)                                                                                                                                                                                                    gCAATAAATGATACGCCATACGTTGCGCGGATTTCGGACCGACGCCCGGTAATACGCGTAAACTTTCGATCAGATTTTCTAACAAGGGGCTGGTTTGCATAAGGTTCCCGATGGATTTGCTGAAAAAATAAACCGCACTTTGCCGTTAATGAAGAAAAAGTGCGGTCGTTTTTTAGGACGTTTTTTAAAACGGCATTTTGAAGCCCGGCGGTAAAGAc                                                                  >  1:94641/1‑218 (MQ=255)                                                                                                                                                                                                    gCAATAAATGATACGCCATACGTTGCGCGGATTTCGGACCGACGCCCGGTAATACGCGTAAACTTTCGATCAGATTTTCTAACAAGGGGCTGGTTTGCATAAGGTTCCCGATGGATTTGCTGAAAAAATAAACCGCACTTTGCCGTTAATGAAGAAAAAGTGCGGTCGTTTTTTAGGACGTTTTTTAAAACGGCATTTTGAAGCCCGGCGGTAAAGACATAc                                                              >  1:343675/1‑222 (MQ=255)                                                                                                                                                                                                    gCAATAAATGATACGCCATACGTTGCGCGGATTTCGGACCGACGCCCGGTAATACGCGTAAACTTTCGATCAGATTTTCTAACAAGGGGCTGGTTTGCATAAGGTTCCCGATGGATTTGCTGAAAAAATAAACCGCACTTTGCCGTTAATGAAGAAAAAGTGCGGTCGTTTTTTAGGACGTTTTTTAAAACGGCATTTTGAAGCCCGGCGGTAAAGACATACCGGCGGTGACTGACGCCATTTTTTCtttt                                 >  1:211034/1‑251 (MQ=255)                                                                                                                                                                                                    gCAATAAATGATACGCCATACGTTGCGCGGATTTCGGACCGACGCCCGGTAATACGCGTAAACTTTCGATCAGATTTTCTAACAAGGGGCTGGTTTGCATAAGGTTCCCGATGGATTTGCTGAAAAAATAAACCGCACTTTGCCGTTAATGAAGAAAAAGTGCGGTCGTTTTTTAGGACGTTTTTTAAAACGGCATTTTGAAGCCCGGCGGTAAAGACATACCGGCGGTGACTGACGCCATTTTTTCtttt                                 >  1:585261/1‑251 (MQ=255)                                                                                                                                                                                                         aaaTGATACGCCATACGTTGCGCGGATTTCGGACCGACGCCCGGTAATACGCGTAAACTTTCGATCAGATTTTCTAACAAGGGGCTGGTTTGCATAAGGTTCCCGATGGATTTGCTGAAAAAATAAACCGCACTTTGCCGTTAATGAAGAAAAAGTGCGGTCGTTTTTTAGGACGTTTTTTAAAACGGCATTTTGAAGCCCGGCGGTAAAGACATACcggcgg                                                        <  1:32637/223‑1 (MQ=255)                                                                                                                                                                                                           aTGATACGCCATACGTTGCGCGGATTTCGGACCGACGCCCGGTAATACGCGTAAACTTTCGATCAGATTTTCTAACAAGGGGCTGGTTTGCATAAGGTTCCCGATGGATTTGCTGAAAAAATAAACCGCACTTTGCCGTTAATGAAGAAAAAGTGCGGTCGTTTTTTAGGACGTTTTTTAAAACGGCATTTTGAAGCCCGGCGGTAAAGACATACCGGCGGTGACTGACGCCATTTTTTCTTTTTGTaa                            >  1:226213/1‑249 (MQ=255)                                                                                                                                                                                                            tGATACGCCATACGTTGCGCGGATTTCGGACCGACGCCCGGTAATACGCGTAAACTTTCGATCAGATTTTCTAACAAGGGGCTGGTTTGCATAAGGTTCCCGATGGATTTGCTGAAAAAATAAACCGCACTTTGCCGTTAATGAAGAAAAAGTGCGGTCGTTTTTTAGGACGTTTTTTAAAACGGCATTTTGAAGCCCGGCGGTAAAGACATAcc                                                             <  1:227300/215‑1 (MQ=255)                                                                                                                                                                                                                aCGCCATACGTTGCGCGGATTTCGGACCGACGCCCGGTAATACGCGTAAAc                                                                                                                                                                                                                             >  1:755960/1‑51 (MQ=255)                                                                                                                                                                                                                aCGCCATACGTTGCGCGGATTTCGGACCGACGCCCGGTAATACGCGTAAACTTTCGATCAGATTTTCTAACAAGGGGCTGGTTTGCATAAGGTTCCCGATGGATTTGCTGAAAAAATAAACCGCACTTTGCCGTTAATGAAGAAAAAGTGCGGTCGTTTTTTAGGACGTTTTTTAAAAc                                                                                             <  1:453824/179‑1 (MQ=255)                                                                                                                                                                                                                aCGCCATACGTTGCGCGGATTTCGGACCGACGCCCGGTAATACGCGTAAACTTTCGATCAGATTTTCTAACAAGGGGCTGGTTTGCATAAGGTTCCCGATGGATTTGCTGAAAAAATAAACCGCACTTTGCCGTTAATGAAGAAAAAGTGCGGTCGTTTTTTAGGACGTTTTTTAAAACGGCATTTTGAAGCCCGGCGGTAAAGACATACCGGCGGTGACTGACGCCATTTTTTCTTTTTGTAAc                           <  1:305727/245‑1 (MQ=255)                                                                                                                                                                                                                  gCCATACGTTGCGCGGATTTCGGACCGACGCCCGGTAATACGCGTAAACTTTCGATCAGATTTTCTAACAAGGGGCTGGTTTGCATAAGGTTCCCGATGGATTTGCTGAAAAAATAAACCGCACTTTGCCGTTAATGAAGAAAAAGTGCGGTCGTTTTTTAGGACGTTTTTTAAAACGGCATTTTGAAGCCCGGCGGTAAAGACATACCGGCGGTGACTGACGCCCTTTTTTTTTTTTGTAACTCTTCgg                    >  1:641553/1‑250 (MQ=255)                                                                                                                                                                                                                  gCCATACGTTGCGCGGATTTCGGACCGACGCCCGGTAATACGCGTAAACTTTCGATCAGATTTTCTAACAAGGGGCTGGTTTGCATAAGGTTCCCGATGGATTTGCTGAAAAAATAAACCGCACTTTGCCGTTAATGAAGAAAAAGTGCGGTCGTTTTTTAGGACGTTTTTTAAAACGGCATTTTGAAGCCCGGCGGTAAAGACATACCGGCGGTGACTGACGCCATTTTTTCTTTTTGTAACTCTTCgg                    >  1:761934/1‑250 (MQ=255)                                                                                                                                                                                                                   ccATACGTTGCGCGGATTTCGGACCGACGCCCGGTAATACGCGTAAACTTTCGATCAGATTTTCTAACAAGGGGCTGGTTTGCATAAGGTTCCCGATGGATTTGCTGAAAAAATAAACCGCACTTTGCCGTTAATGAAGAAAAAGTGCGGTCGTTTTTTAGGACGTTTTTTAAAACGGCATTTTGAAGCCCGGCGGTAAAGACATACCGGCGGTGGCCGGCGCCCTTTTTTTTTTTTGTAACTCTTCggga                  >  1:57172/1‑249 (MQ=255)                                                                                                                                                                                                                       aCGTTGCGCGGATTTCGGACCGACGCCCGGTAATACGCGTAAACTTTCGATCAGATTTTCTAACAAGGGGCTGGtt                                                                                                                                                                                             >  1:416356/1‑76 (MQ=255)                                                                                                                                                                                                                           tGCGCGGATTTCGGACCGACGCCCGGTAATACGCGTAAACTTTCGATCAGATTTTCTAACAAGGGGCTGGTTTGCATAAGGTTCCCGATGGATTTGCTGAAAAAATAAACCGCACTTTGCCGTTAATGAAGAAAAAGTGCGGTCGTTTTTTAGGACGTTTTTTAAAACGGCATTTTGAAGCCCGGCGGTAAAGACATACCGGCGGTGACTGACGCCATTTTTTCTTTTTGTAACTCTTCGGCACGACGCAc          <  1:663593/251‑1 (MQ=255)                                                                                                                                                                                                                               cgGATTTCGGACCGACGCCCGGTAATACGCGTAAACTTTCGATCAGATTTTCTAACAAGGGGCTGGTTTGCATAAGGTTCCCGATGGATTTGCTGAAAAAATAAACCGCACTTTGCCGTTAATGAAGAAAAAGTGCGGTCGTTTTTTAGGACGTTTTTTAAAACGGCATTTTGAAGCCCGGCGGTAAAGACATACCGGCGGTGACTGACGCCATTTTTTCTTTTTGTAACTCTTCGGCACGACGCACTGc       <  1:660971/250‑1 (MQ=255)                                                                                                                                                                                                                                 gATTTCGGACCGACGCCCGGTAATACGCGTAAACTTTCGATCAGATTTTCTAACAAGGGGCTGGTTTGCATAAGGTTCCCGATGGATTTGCTGAAAAAATAAACCGCACTTTGCCGTTAATGAAGAAAAAGTGCGGTCGTTTTTTAGGACGTTTTTTAAAAc                                                                                             <  1:312934/162‑1 (MQ=255)                                                                                                                                                                                                                                 gATTTCGGACCGACGCCCGGTAATACGCGTAAACTTTCGATCAGATTTTCTAACAAGGGGCTGGTTTGCATAAGGTTCCCGATGGATTTGCTGAAAAAATAAACCGCACTTTGCCGTTAATGAAGAAAAAGTGCGGTCGTTTTTTAGGACGTTTTTTAAAACGGCATTTTGa                                                                                   <  1:46033/172‑1 (MQ=255)                                                                                                                                                                                                                                 gATTTCGGACCGACGCCCGGTAATACGCGTAAACTTTCGATCAGATTTTCTAACAAGGGGCTGGTTTGCATAAGGTTCCCGATGGATTTGCTGAAAAAATAAACCGCACTTTGCCGTTAATGAAGAAAAAGTGCGGTCGTTTTTTAGGACGTTTTTTAAAACGGCATTTTGAAGCCCGGCGGTAAAGACATACCGGCGGTGACTGACGCCATTTTTTCTTTTTTTAACTCTTCGGCACGACGCACTGCAt     >  1:260896/1‑250 (MQ=255)                                                                                                                                                                                                                                 gATTTCGGACCGACGCCCGGTAATACGCGTAAACTTTCGATCAGATTTTCTAACAAGGGGCTGGTTTGCATAAGGTTCCCGATGGATTTGCTGAAAAAATAAACCGCACTTTGCCGTTAATGAAGAAAAAGTGCGGTCGTTTTTTAGGACGTTTTTTAAAACGGCATTTTGAAGCCCGGCGGTAAAGACATACCGGCGGTGACTGACGCCATTTTTTCTTTTTGTAACTCTTCGGCACGACGCACTGCAt     <  1:823582/250‑1 (MQ=255)                                                                                                                                                                                                                                 gATTTCGGACCGACGCCCGGTAATACGCGTAAACTTTCGATCAGATTTTCTAACAAGGGGCTGGTTTGCATAAGGTTCCCGATGGATTTGCTGAAAAAATAAACCGCACTTTGCCGTTAATGAAGAAAAAGTGCGGTCGTTTTTTAGGACGTTTTTTAAAACGGCATTTTGAAGCCCGGCGGTAAAGACATACCGGCGGTGACTGACGCCATTTTTTCTTTTTGTAACTCTTCGGCACGACGCACTGCAt     <  1:564953/250‑1 (MQ=255)                                                                                                                                                                                                                                 gATTTCGGACCGACGCCCGGTAATACGCGTAAACTTTCGATCAGATTTTCTAACAAGGGGCTGGTTTGCATAAGGTTCCCGATGGATTTGCTGAAAAAATAAACCGCACTTTGCCGTTAATGAAGAAAAAGTGCGGTCGTTTTTTAGGACGTTTTTTAAAACGGCATTTTGAAGCCCGGCGGTAAAGACATACCGGCGGTGACTGACGCCATTTTTTCTTTTTGTAACTCTTCGGCACGACGCACTGCAt     <  1:869395/250‑1 (MQ=255)                                                                                                                                                                                                                                        gACCGACGCCCGGTAATACGCGTAAACTTTCGATCAGATTTTCTAACAAGGGGCTGGTTTGCATAAGGTTCCCGATGGATTTGCTGAAAAAATAAACCGCACTTTGCCGTTAATGAAGAAAAAGTGCGGTCGTTTTTTAGGACGTTTTTTAAAACGGCATTTTGAAGCCCGGCGGTAAAGACATACCGGCGGTGACTGACGc                                              <  1:578501/202‑1 (MQ=255)                                                                                                                                                                                                                                               gCCCGGTAATACGCGTAAAC‑TTCGATCAGATTTTCTAACAAGGGGCTGGTTTGCATAAGGTTCCCGATGGATTTGCTGAAAAAATAAACCGCACTTTGCCGTTAATGAAGAAAAAGTGCGGTCGTTTTTTAGGACGTTTTTTAAAACGGCATTTTGAAGCCCGGCGGTAAAGACATACCGGCGGTGACTGACGCCATTTTTTCTTTTTGTAACTCTTCGGCACGACGCACTGCATCGt  <  1:263286/238‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                         CTGTTTGCTCGATAGCAAGAATATCCGCCGGCGTTTCCACCACGCAAAGCAAGCCCGAATTTTGGCGGCGTGGATTACTGCAAATGTTGCACACGTCTTCTTCCGTAAAAGTGCGGCAATGTTCGCAATGCCCGATTTTCGACATCGCTTCCGTCAGCCCTTGCGCCAAGCGCATTCCGCCGCTGCGATTGCGTTGCAATAAATGATACGCCATACGTTGCGCGGATTTCGGACCGACGCCCGGCAATACGCGTAAACTTTCGATCAGATTTTCTAACAAGGGGCTGGTTTGCATAAGGTTCCCGATGGATTTGCTGAAAAAATAAACCGCACTTTGCCGTTAATAAAGAAAAAGTGCGGTCGTTTTTTAGGACGTTTTTTAAAACGGCATTTTGAAGCCCGGCGGTAAAGACATACCGGCGGTGACTGACGCCATTTTTTCTTTTTGTAACTCTTCGGCACGACGCACTGCATCGT  >  1/1420239‑1420715

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑