Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,413,541	G→T	A32S (GCG→TCG)	unknown →	Soluble lytic murein transglycosylase (EC 4.2.2.n1)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,413,541	0	G	T	100.0%	205.2 / NA	77	A32S (GCG→TCG)	unknown	Soluble lytic murein transglycosylase (EC 4.2.2.n1)
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (49/28);  total (49/28)

TCTCCCGACGCATGCTTATATCACGTAATAATATTAATAAATTAAAATACAGCCGTCGGCTTTAACAACGATATTTCAGCTTTCAGCCCATTTTCAAAGGCAGACTTACTATTCGCCTTTCCCATATTCACCACAGGAAAATAACTATGCGATATTTGAAACAAACAACAATTTCACTGTTAATTTTGACCGCACTTTCCTCCTCCTTTGCCAATCAGCACAAGGCGACAACGCATAAAGCGAATGTTGCCCATACGCACGCCAAACCGGAGCAACACCACGCAGAATTAAAACGGCTAAAACAGCGTGCAACTTTTCTGCAGTTAGAAAGCCTGCTGAAAAGTGCGGTCAAAAATAACGGCGTTTTTATCAACCAAACTGTATTCCTGAAACTGATTGAGGATTTGAAAGGCTATCCGTTGCAAACAGATGCCATAGCGGCTTATTTCGACGCCTGCATTAAAAGCGTAAATCACGACACA  >  1/1413302‑1413783                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                   tctcCCGACGCATGCTTATATCACGTAATAATATTAATAAATTAAAATACAGCCGTCGGCTTTAACAACGATATTTCAGCTTTCAGCCCATTTTCAAAGGCAGACTTACTATTCGCCTTTCCCATATTCACCACAGGAAAATAACTATGCGATATTTGAAACAAACAACAATTTCACTGTTAATTTTGACCGCACTTTCCTCCTCCTTTGCCAATCAGCACAAGGCGACAACGCATAAATCGAATGTTGcc                                                                                                                                                                                                                                         <  1:522805/251‑1 (MQ=255)    cccGACGCATGCTTATATCACGTAATAATATTAATAAATTAAAATACAGCCGTCGGCTTTAACAACGATATTTCAGCTTTCAGCCCATTTTCAAAGGCAGACTTACTATTCGCCTTTCCCATATTCACCACAGGAAAATAACTATGCGATATTTGAAACAAACAACAATTTCACTGTTAATTTTGACCGCACTTTCCTCCTCCTTTGCCAATCAGCACAAGGCGACAACGCATAAATCGAATGTTGCCCAt                                                                                                                                                                                                                                      >  1:812452/1‑251 (MQ=255)             tGCTTATATCACGTAATAATATTAATAAATTAAAATACAGCCGTCGGCTTTAACAACGATATTTCAGCTTTCAGCCCATTTTCAAAGGCAGACTTACTATTCGCCTTTCCCATATTCACCACAGGAAAATAACTATGCGATATTTGAAACAAACAACAATTTCACTGTTAATTTTGACCGCACTTTCCTCCTCCTTTGCCAATCAGCACAAGGCGACAACGCATAAATCGAATGTTGCCCATacgcacgc                                                                                                                                                                                                                              >  1:11141/1‑250 (MQ=255)             tGCTTATATCACGTAATAATATTAATAAATTAAAATACAGCCGTCGGCTTTAACAACGATATTTCAGCTTTCAGCCCATTTTCAAAGGCAGACTTACTATTCGCCTTTCCCATATTCACCACAGGAAAATAACTATGCGATATTTGAAACAAACAACAATTTCACTGTTAATTTTGACCGCACTTTCCTCCTCCTTTGCCAATCAGCACAAGGCGACAACGCATAAATCGAATGTTGCCCATacgcacgc                                                                                                                                                                                                                              >  1:52407/1‑250 (MQ=255)             tGCTTATATCACGTAATAATATTAATAAATTAAAATACAGCCGTCGGCTTTAACAACGATATTTCAGCTTTCAGCCCATTTTCAAAGGCAGACTTACTATTCGCCTTTCCCATATTCACCACAGGAAAATAACTATGCGATATTTGAAACAAACAACAATTTCACTGTTAATTTTGACCGCACTTTCCTCCTCCTTTGCCAATCAGCACAAGGCGACAACGCATAAATCGAATGTTGCCCATacgcacgc                                                                                                                                                                                                                              >  1:272406/1‑250 (MQ=255)                        cGTAATAATATTAATAAATTAAAATACAGCCGTCGGCTTTAACAACGATATTTCAGCTTTCAGCCCATTTTCAAAGGCAGACTTACTATTCGCCTTTCCCATATTCACCACAGGAAAATAACTATGCGATATTTGAAACAAACAACAATTTCACTGTTAATTTTGACCGCACTTTCCTCCTCCTTTGCCAATCAGCACAAGGCGACAACGCATAAATCGAATGTTGCCCATacg                                                                                                                                                                                                                                   <  1:560535/234‑1 (MQ=255)                          taataaTATTAATAAATTAAAATACAGCCGTCGGCTTTAACAACGATATTTCAGCTTTCAGCCCATTTTCAAAGGCAGACTTACTATTCGCCTTTCCCATATTCACCACAGGAAAATAACTATGCGATATTTGAAACAAACAACAATTTCACTGTTAATTTTGACCGCACTTTCCTCCTCCTTTGCCAATCAGCACAAGGCGACAACGCATAAATCGAATGTTGCCCATACGCACGCCAAACCGGAGCaa                                                                                                                                                                                                                 >  1:791197/1‑250 (MQ=255)                            ataataTTAATAAATTAAAATACAGCCGTCGGCTTTAACAACGATATTTCAGCTTTCAGCCCATTTTCAAAGGCAGACTTACTATTCGCCTTTCCCATATTCACCACAGGAAAATAACTATGCGATATTTGAAACAAACAACAATTTCACTGTTAATTTTGACCGCACTTTCCTCCTCCTTTGCCAATCAGCACAAGGCGACAACGCATAAATCGAATGTTGCCCATACGCACGCCAAACCGGAGCAacac                                                                                                                                                                                                              <  1:228596/251‑1 (MQ=255)                            ataataTTAATAAATTAAAATACAGCCGTCGGCTTTAACAACGATATTTCAGCTTTCAGCCCATTTTCAAAGGCAGACTTACTATTCGCCTTTCCCATATTCACCACAGGAAAATAACTATGCGATATTTGAAACAAACAACAATTTCACTGTTAATTTTGACCGCACTTTCCTCCTCCTTTGCCAATCAGCACAAGGCGACAACGCATAAATCGAATGTTGCCCATACGCACGCCAAACCGGAGCAaca                                                                                                                                                                                                               >  1:748113/1‑250 (MQ=255)                                 atTAATAAATTAAAATACAGCCGTCGGCTTTAACAACGATATTTCAGCTTTCAGCCCATTTTCAAAGGCAGACTTACTATTCGCCTTTCCCATATTCACCACAGGAAAATAACTATGCGATATTTGAAACAAACAACAATTTCACTGTTAATTTTGACCGCACTTTCCTCCTCCTTTGCCAATCAGCACAAGGCGACAACGCATAAATCGAATGTTGCCCATACGCACGCCAAACCGGAGCAACACCACGc                                                                                                                                                                                                         <  1:494936/251‑1 (MQ=255)                                        aaTTAAAATACAGCCGTCGGCTTTAACAACGATATTTCAGCTTTCAGCCCATTTTCAAAGGCAGACTTACTATTCGCCTTTCCCATATTCACCACAGGAAAATAACTATGCGATATTTGAAACAAACAACAATTTCACTGTTAATTTTGACCGCACTTTCCTCCTCCTTTGCCAATCAGCACAAGGCGACAACGCATAAATCGAATGTTGCCCATACGCACGCCAAACCGGAGCAACACCACGCAGAATTa                                                                                                                                                                                                  >  1:544407/1‑251 (MQ=255)                                         aTTAAAATACAGCCGTCGGCTTTAACAACGATATTTCAGCTTTCAGCCCATTTTCAAAGGCAGACTTACTATTCGCCTTTCCCATATTCACCACAGGAAAATAACTATGCGATATTTGAAACAAACAACAATTTCACTGTTAATTTTGACCGCACTTTCCTCCTCCTTTGCCAATCAGCACAAGGCGACAACGCATAAATCGAATGTTGCCCATACGCACGCCAAACCGGAGCAACACCACGCAGAATTag                                                                                                                                                                                                 >  1:279538/1‑250 (MQ=255)                                           tAAAATACAGCCGTCGGCTTTAACAACGATATTTCAGCTTTCAGCCCATTTTCAAAGGCAGACTTACTATTCGCCTTTCCCATATTCACCACAGGAAAATAACTATGCGATATTTGAAACAAACAACAATTTCACTGTTAATTTTGACCGCACTTTCCTCCTCCTTTGCCAATCAGCACAAGGCGACAACGCATAAATCGAATGTTGCCCATACGCACGCCAAACCGGAGCAACACCACGCAGAATTagaa                                                                                                                                                                                               <  1:878870/251‑4 (MQ=255)                                           tAAAATACAGCCGTCGGCTTTAACAACGATATTTCAGCTTTCAGCCCATTTTCAAAGGCAGACTTACTATTCGCCTTTCCCATATTCACCACAGGAAAATAACTATGCGATATTTGAAACAAACAACAATTTCACTGTTAATTTTGACCGCACTTTCCTCCTCCTTTGCCAATCAGCACAAGGCGACAACGCATAAATCGAATGTTGCCCATACGCACGCCAAACCGGAGCAACACCACGCAGAATTagaa                                                                                                                                                                                               <  1:367749/251‑4 (MQ=255)                                           tAAAATACAGCCGTCGGCTTTAACAACGATATTTCAGCTTTCAGCCCATTTTCAAAGGCAGACTTACTATTCGCCTTTCCCATATTCACCACAGGAAAATAACTATGCGATATTTGAAACAAACAACAATTTCACTGTTAATTTTGACCGCACTTTCCTCCTCCTTTGCCAATCAGCACAAGGCGACAACGCATAAATCGAATGTTGCCCATACGCACGCCAAACCGGAGCAACACCACGCAGAATTagaa                                                                                                                                                                                               <  1:315818/251‑4 (MQ=255)                                            aaaaTACAGCCGTCGGCTTTAACAACGATATTTCAGCTTTCAGCCCATTTTCAAAGGCAGACTTACTATTCGCCTTTCCCATATTCACCACAGGAAAATAACTATGCGATATTTGAAACAAACAACAATTTCACTGTTAATTTTGACCGCACTTTCCTCCTCCTTTGCCAATCAGCACAAGGCGACAACGCATAAATCGAATGTTGCCCATACGCACGCCAAACCGGAGCAACACCACGCAGAATTAGAAc                                                                                                                                                                                              <  1:336132/251‑1 (MQ=255)                                             aaaTACAGCCGTCGGCTTTAACAACGATATTTCAGCTTTCAGCCCATTTTCAAAGGCAGACTTACTATTCGCCTTTCCCATATTCACCACAGGAAAATAACTATGCGATATTTGAAACAAACAACAATTTCACTGTTAATTTTGACCGCACTTTCCTCCTCCTTTGCCAATCAGCACAAGGCGACAACGCATAAATCGAATGTTGCCCATACGCACGCCAAACCGGAGCAACACCACGCAGAATTagaa                                                                                                                                                                                               >  1:122254/1‑246 (MQ=255)                                             aaaTACAGCCGTCGGCTTTAACAACGATATTTCAGCTTTCAGCCCATTTTCAAAGGCAGACTTACTATTCGCCTTTCCCATATTCACCACAGGAAAATAACTATGCGATATTTGAAACAAACAACAATTTCACTGTTAATTTTGACCGCACTTTCCTCCTCCTTTGCCAATCAGCACAAGGCGACAACGCATAAATCGAATGTTGCCCATACGCACGCCAAACCGGAGCAACACCACGCAGAATTAGAACg                                                                                                                                                                                             >  1:781165/1‑251 (MQ=255)                                             aaaTACAGCCGTCGGCTTTAACAACGATATTTCAGCTTTCAGCCCATTTTCAAAGGCAGACTTACTATTCGCCTTTCCCATATTCACCACAGGAAAATAACTATGCGATATTTGAAACAAACAACAATTTCACTGTTAATTTTGACCGCACTTTCCTCCTCCTTTGCCAATCAGCACAAGGCGACAACGCATAAATCGAATGTTGCCCATACGCACGCCAAACCGGAGCAACACCACGCAGAATTAGAACg                                                                                                                                                                                             >  1:214189/1‑251 (MQ=255)                                                     ccGTCGGCTTTAACAACGATATTTCAGCTTTCAGCCCATTTTCAAAGGCAGACTTACTATTCGCCTTTCCCATATTCACCACAGGAAAATAACTATGCGATATTTGAAACAAACAACAATTTCACTGTTAATTTTGACCGCACTTTCCTCCTCCTTTGCCAATCAGCACAAGGCGACAACGCATAAATCGAATGTTGCCCATACGCACGCCAAACCGGAGCAACACCACGCAGAATTAGAACGGCTAAAAc                                                                                                                                                                                     <  1:683628/251‑1 (MQ=255)                                                        tctgCTTTAACAACGATATTTCAGCTTTCAGCCCATTTTCAAAGGCAGACTTACTATTCGCCTTTCCCATATTCACCACAGGAAAATAACTATGCGATATTTGAAACAAACAACAATTTCACTGTTAATTTTGACCGCACTTTCCTCCTCCTTTGCCAATCAGCACAAGGCGACAACGCATAAATCGAATGTTGCCCATACGCACGCCAAACCGGAGCAACACCACGCAGAATTAGAACGGCTAAAACAg                                                                                                                                                                                   <  1:26765/247‑1 (MQ=255)                                                           gCTTTAACAACGATATTTCAGCTTTCAGCCCATTTTCAAAGGCAGACTTACTATTCGCCTTTCCCATATTCACCACAGGAAAATAACTATGCGATATTTGAAACAAACAACAATTTCACTGTTAATTTTGACCGCACTTTCCTCCTCCTTTGCCAATCAGCACAAGGCGACAACGCATAAATCGAATGTTGCCCATACGCACGCCAAACCGGAGCAACACCACGCAGAATTAGAACGGCTAAAACAGCGTg                                                                                                                                                                               >  1:758984/1‑251 (MQ=255)                                                           gCTTTAACAACGATATTTCAGCTTTCAGCCCATTTTCAAAGGCAGACTTACTATTCGCCTTTCCCATATTCACCACAGGAAAATAACTATGCGATATTTGAAACAAACAACAATTTCACTGTTAATTTTGACCGCACTTTCCTCCTCCTTTGCCAATCAGCACAAGGCGACAACGCATAAATCGAATGTTGCCCATACGCACGCCAAACCGGAGCAACACCACGCAGAATTAGAACGGCTAAAACAGCGTg                                                                                                                                                                               >  1:742161/1‑251 (MQ=255)                                                           gCTTTAACAACGATATTTCAGCTTTCAGCCCATTTTCAAAGGCAGACTTACTATTCGCCTTTCCCATATTCACCACAGGAAAATAACTATGCGATATTTGAAACAAACAACAATTTCACTGTTAATTTTGACCGCACTTTCCTCCTCCTTTGCCAATCAGCACAAGGCGACAACGCATAAATCGAATGTTGCCCATACGCACGCCAAACCGGAGCAACACCACGCAGAATTAGAACGGCTAAAACAGCGTg                                                                                                                                                                               >  1:387010/1‑251 (MQ=255)                                                             tttAACAACGATATTTCAGCTTTCAGCCCATTTTCAAAGGCAGACTTACTATTCGCCTTTCCCATATTCACCACAGGAAAATAACTATGCGATATTTGAAACAAACAACAATTTCACTGTTAATTTTGACCGCACTTTCCTCCTCCTTTGCCAATCAGCACAAGGCGACAACGCATAAATCGAATGTTGCCCATACGCACGCCAAACCGGAGCAACACCACGCAGAATTAGAACGGCTAAAACAGCGTGCa                                                                                                                                                                             >  1:223149/1‑251 (MQ=255)                                                                    acGATATTTCAGCTTTCAGCCCATTTTCAAAGGCAGACTTACTATTCGCCTTTCCCATATTCACCACAGGAAAATAACTATGCGATATTTGAAACAAACAACAATTTCACTGTTAATTTTGACCGCACTTTCCTCCTCCTTTGCCAATCAGCACAAGGCGACAACGCATAAATCGAATGTTGCCCATACGCACGCCAAACCGGAGCAACACCACGCAGAATTAGAACGGCTAAAACAGCGTGCAACtttt                                                                                                                                                                       >  1:817872/1‑250 (MQ=255)                                                                    acGATATTTCAGCTTTCAGCCCATTTTCAAAGGCAGACTTACTATTCGCCTTTCCCATATTCACCACAGGAAAATAACTATGCGATATTTGAAACAAACAACAATTTCACTGTTAATTTTGACCGCACTTTCCTCCTCCTTTGCCAATCAGCACAAGGCGACAACGCATAAATCGAATGTTGCCCATACGCACGCCAAACCGGAGCAACACCACGCAGAATTAGAACGGCTAAAACAGCGTGCAACtttt                                                                                                                                                                       >  1:511659/1‑250 (MQ=255)                                                                         atTTCAGCTTTCAGCCCATTTTCAAAGGCAGACTTACTATTCGCCTTTCCCATATTCACCACAGGAAAATAACTATGCGATATTTGAAACAAACAACAATTTCACTGTTAATTTTGACCGCACTTTCCTCCTCCTTTGCCAATCAGCACAAGGCGACAACGCATAAATCGAATGTTGCCCATACGCACGCCAAACCGGAGCAACACCACGCAGAATTAGAACGGCTAAAACAGCGTGCAACTTTTCTGCAg                                                                                                                                                                 <  1:170593/251‑1 (MQ=255)                                                                                          aTTTTCAAAGGCAGACTTACTATTCGCCTTTCCCATATTCACCACAGGAAAATAACTATGCGATATTTGAAACAAACAACAATTTCACTGTTAATTTTGACCGCACTTTCCTCCTCCTTTGCCAATCAGCACAAGGCGACAACGCATAAATCGAATGTTGCCCATACGCACGCCAAACCGGAGCAACACCACGCAGAATTAGAACGGCTAAAACAg                                                                                                                                                                                   >  1:611448/1‑216 (MQ=255)                                                                                            tttCAAAGGCAGACTTACTATTCGCCTTTCCCATATTCACCACAGGAAAATAACTATGCGATATTTGAAACAAACAACAATTTCACTGTTAATTTTGACCGCACTTTCCTCCTCCTTTGCCAATCAGCACAAGGCGACAACGCATAAATCGAATGTTGCCCATACGCACGCCAAACCGGAGCAACACCACGCAGAATTAGAACGGCTAAAACAGCGTGCAACTTTTCTGCAGTTAGAAAGCCTGCTGaaaa                                                                                                                                              >  1:757756/1‑251 (MQ=255)                                                                                             ttCAAAGGCAGACTTACTATTCGCCTTTCCCATATTCACCACAGGAAAATAACTATGCGATATTTGAAACAAACAACAATTTCACTGTTAATTTTGACCGCACTTTCCTCCTCCTTTGCCAATCAGCACAAGGCGACAACGCATAAATCGAATGTTGCCCATACGCACGCCAAACCGGAGCAACACCACGCAGAATTAGAACGGCTAAAACAGCGTGCAACTTTTCTGCAGTTAGAAAGCCTGCTGAAAAg                                                                                                                                             <  1:523205/251‑1 (MQ=255)                                                                                                       gACTTACTATTCGCCTTTCCCATATTCACCACAGGAAAATAACTATGCGATATTTGAAACAAACAACAATTTCACTGTTAATTTTGACCGCACTTTCCTCCTCCTTTGCCAATCAGCACAAGGCGACAACGCATAAATCGAATGTTGCCCATACGCACGCCAAAcc                                                                                                                                                                                                                        >  1:685831/1‑166 (MQ=255)                                                                                                       gACTTACTATTCGCCTTTCCCATATTCACCACAGGAAAATAACTATGCGATATTTGAAACAAACAACAATTTCACTGTTAATTTTGACCGCACTTTCCTCCTCCTTTGCCAATCAGCACAAGGCGACAACGCATAAATCGAATGTTGCCCATACGCACGCCAAACCGGAGCAACACCACGCAGAATTagaa                                                                                                                                                                                               <  1:6379/191‑4 (MQ=255)                                                                                                       gACTTACTATTCGCCTTTCCCATATTCACCACAGGAAAATAACTATGCGATATTTGAAACAAACAACAATTTCACTGTTAATTTTGACCGCACTTTCCTCCTCCTTTGCCAATCAGCACAAGGCGACAACGCATAAATCGAATGTTGCCCATACGCACGCCAAACCGGAGCAACACCACGCAGAATTAGAACGGCTAAAACAGCGTGCAACTTTTCTGCAGTTAGAAAGCCTGCTGAAAAGTGCGGTCaaa                                                                                                                                   >  1:379127/1‑251 (MQ=255)                                                                                                       gACTTACTATTCGCCTTTCCCATATTCACCACAGGAAAATAACTATGCGATATTTGAAACAAACAACAATTTCACTGTTAATTTTGACCGCACTTTCCTCCTCCTTTGCCAATCAGCACAAGGCGACAACGCATAAATCGAATGTTGCCCATACGCACGCCAAACCGGAGCAACACCACGCAGAATTAGAACGGCTAAAACAGCGTGCAACTTTTCTGCAGTTAGAAAGCCTGCTGAAAAGTGCGGTCaaa                                                                                                                                   >  1:491609/1‑251 (MQ=255)                                                                                                        aCTTACTATTCGCCTTTCCCATATTCACCACAGGAAAATAACTATGCGATATTTGAAACAAACAACAATTTCACTGTTAATTTTGACCGCACTTTCCTCCTCCTTTGCCAATCAGCACAAGGCGACAACGCATAAATCGAATGTTGCCCATACGCACGCCAAACCGGAGCAACACCACGCAGAATTAGAACGGCTAAAACAGCGTGCAACTTTTCTGCAGTTAGAAAGCCTGCTGAAAAGTGCGGTCaaaa                                                                                                                                  >  1:334984/1‑251 (MQ=255)                                                                                                        aCTTACTATTCGCCTTTCCCATATTCACCACAGGAAAATAACTATGCGATATTTGAAACAAACAACAATTTCACTGTTAATTTTGACCGCACTTTCCTCCTCCTTTGCCAATCAGCACAAGGCGACAACGCATAAATCGAATGTTGCCCATACGCACGCCAAACCGGAGCAACACCACGCAGAATTAGAACGGCTAAAACAGCGTGCAACTTTTCTGCAGTTAGAAAGCCTGCTGAAAAGTGCGGTCa                                                                                                                                     <  1:102250/248‑1 (MQ=255)                                                                                                         cTTACTATTCGCCTTTCCCATATTCACCACAGGAAAATAACTATGCGATATTTGAAACAAACAACAATTTCACTGTTAATTTTGACCGCACTTTCCTCCTCCTTTGCCAATCAGCACAAGGCGACAACGCATAAATCGAATGTTGCCCATACGCACGCCAAACCGGAGCAACACCACGCAGAATTAGAACGGCTAAAACAGCGTGCAACTTTTCTGCAGTTAGAAAGCCTGCTGAAAAGTGCGGTCaaaaa                                                                                                                                 >  1:556201/1‑251 (MQ=255)                                                                                                                       ttCCCATATTCACCACAGGAAAATAACTATGCGATATTTGAAACAAACAACAATTTCACTGTTAATTTTGACCGCACTTTCCTCCTCCTTTGCCAATCAGCACAAGGCGACAACGCATAAATCGAATGt                                                                                                                                                                                                                                             <  1:334243/129‑1 (MQ=255)                                                                                                                       ttCCCATATTCACCACAGGAAAATAACTATGCGATATTTGAAACAAACAACAATTTCACTGTTAATTTTGACCGCACTTTCCTCCTCCTTTGCCAATCAGCACAAGGCGACAACGCATAAATCGAATGTTGCCCATACGCACGCCAAACCGGAGCAACACCACGCAGAATTAGAACGGCTAAAACAGCGTGCAACTTTTCTGc                                                                                                                                                                   >  1:308210/1‑203 (MQ=255)                                                                                                                        tCCCATATTCACCACAGGAAAATAACTATGCGATATTTGAAACAAACAACAATTTCACTGTTAATTTTGACCGCACTTTCCTCCTCCTTTGCCAATCAGCACAAGGCGACAACGCATAAATCGAATGTTGCCCATACGCACGCCAAACCGGAGCAACACCACGCAGAATTAGAACGGCTAAAACAGCGTGCAACTTTTCTGCAGTTAGAAAGCCTGCTGAAAAGTGCGGTCAAAAATAACGGCGTTTTTAt                                                                                                                  <  1:626853/251‑1 (MQ=255)                                                                                                                         cccATATTCACCACAGGAAAATAACTATGCGATATTTGAAACAAACAACAATTTCACTGTTAATTTTGACCGCACTTTCCTCCTCCTTTGCCAATCAGCACAAGGCGACAACGCATAAATCGAATGTTGCCCATACGCACGCCAAACCGGAGCAACACCACGc                                                                                                                                                                                                         >  1:51873/1‑163 (MQ=255)                                                                                                                                   ccacAGGAAAATAACTATGCGATATTTGAAACAAACAACAATTTCACTGTTAATTTTGACCGCACTTTCCTCCTCCTTTGCCAATCAGCACAAGGCGACAACGCATAAATCGAATGTTGCCCATacg                                                                                                                                                                                                                                   <  1:446202/127‑1 (MQ=255)                                                                                                                                   ccacAGGAAAATAACTATGCGATATTTGAAACAAACAACAATTTCACTGTTAATTTTGACCGCACTTTCCTCCTCCTTTGCCAATCAGCACAAGGCGACAACGCATAAATCGAATGTTGCCCATACGCACGCCAAACCGGAGCAACACCACGCAGAATTAGAACGGCTAAAACAGCGTGCAACTTTTCTGCAGTTAGAAAGCCTGCTGAAAAGTGCGGTCAAAAAt                                                                                                                                >  1:625528/1‑226 (MQ=255)                                                                                                                                      caGGAAAATAACTATGCGATATTTGAAACAAACAACAATTTCACTGTTAATTTTGACCGCACTTTCCTCCTCCTTTGCCAATCAGCACAAGGCGACAACGCATAAATCGAATGTTGCCCATACGCACGCCAAACCGGAGCAACACCACGCAGAAt                                                                                                                                                                                                    <  1:471416/155‑1 (MQ=255)                                                                                                                                        ggAAAATAACTATGCGATATTTGAAACAAACAACAATTTCACTGTTAATTTTGACCGCACTTTCCTCCTCCTTTGCCAATCAGCACAAGGCGACAACGCATAAATCGAATGTTGCCCATACGCACGCCAAACCGGAGCAACACCACGCAGAATTAGAACGGCTAAAACAGCGTGCAACTTTTCTGCAGTTAGaa                                                                                                                                                           <  1:651944/194‑1 (MQ=255)                                                                                                                                          aaaaTAACTATGCGATATTTGAAACAAACAACAATTTCACTGTTAATTTTGACCGCACTTTCCTCCTCCTTTGCCAATCAGCACAAGGCGACAACGCATAAATCGAATGTTGCCCATACGCACGCCAAACCGGAGCAACACCACGCAGAATTAGAACGGCTAAAACAGCGTGCAACTTTTCTGCAGTTAGAAAGCCTGCTGAAAAGTGCGGTCAAAAATAACGGCGTTTTTATCAACCAAACTGTATTcc                                                                                                 <  1:570122/250‑1 (MQ=255)                                                                                                                                                   aTGCGATATTTGAAACAAACAACAATTTCACTGTTAATTTTGACCGCACTTTCCTCCTCCTTTGCCAATCAGCACAAGGCGACAACGCATAAATCGAATGTTGCCCATACGCACGCCAAACCGGAGCAACACCACGCAGAATTAGAACGGCTAAAACAGCGTGCAACTTTTCTGCAGTTAGAAAGCCTGCTGAAAAGTGCGGTCAAAAATAACGGCGTTTTTATCAACCAAACTg                                                                                                       >  1:462756/1‑235 (MQ=255)                                                                                                                                                        atatTTGAAACAAACAACAATTTCACTGTTAATTTTGACCGCACTTTCCTCCTCCTTTGCCAATCAGCACAAGGCGACAACGCATAAATCGAATGTTGCCCATACGCACGCCAAACCGGAGCAACACCACGCAGAATTAGAACGGCTAAAACAGCGTGCAACTTTTCTGCAGTTAGAAAg                                                                                                                                                         >  1:753308/1‑180 (MQ=255)                                                                                                                                                        atatTTGAAACAAACAACAATTTCACTGTTAATTTTGACCGCACTTTCCTCCTCCTTTGCCAATCAGCACAAGGCGACAACGCATAAATCGAATGTTGCCCATACGCACGCCAAACCGGAGCAACACCACGCAGAATTAGAACGGCTAAAACAGCGTGCAACTTTTCTGCAGTTAGAAAGCCTGCTGAAAAGTGCGGTCAAAAATAACGGCGTTTTTATCAACCAAACTGTATTCCTGAAACTGATTGAgg                                                                                  >  1:227314/1‑251 (MQ=255)                                                                                                                                                         tatTTGAAACAAACAACAATTTCACTGTTAATTTTGACCGCACTTTCCTCCTCCTTTGCCAATCAGCACAAGGCGACAACGCATAAATCGAATGTTGCCCATACGCACGCCAAACCGGAGCAACACCACGCAGAATTAGAACGGCTAAAACAGCGTGCAACTTTTCTGCAGTTAGAAAGCCTGCTGAAAAGTGCGGTCAAAAATAACGGCGTTTTTATCAACCAAACTGTATTCCTGAAACTGATTGAGGa                                                                                 <  1:566636/251‑1 (MQ=255)                                                                                                                                                          atTTGAAACAAACAACAATTTCACTGTTAATTTTGACCGCACTTTCCTCCTCCTTTGCCAATCAGCACAAGGCGACAACGCATAAATCGAATGTTGCCCATACGCACGCCAAACCGGAGCAACACCACGCAGAATTAGAACGGCTAAAACAGCGTGCAACTTTTCTGCAGTTAGAAAGCCTGCTGAAAAGTGCGGTCa                                                                                                                                     >  1:332028/1‑198 (MQ=255)                                                                                                                                                          atTTGAAACAAACAACAATTTCACTGTTAATTTTGACCGCACTTTCCTCCTCCTTTGCCAATCAGCACAAGGCGACAACGCATAAATCGAATGTTGCCCATACGCACGCCAAACCGGAGCAACACCACGCAGAATTAGAACGGCTAAAACAGCGTGCAACTTTTCTGCAGTTAGAAAGCCTGCTGAAAAGTGCGGTCAAAAATAACgg                                                                                                                           >  1:177391/1‑208 (MQ=255)                                                                                                                                                                      caacaaTTTCACTGTTAATTTTGACCGCACTTTCCTCCTCCTTTGCCAATCAGCACAAGGCGACAACGCATAAATCGAATGTTGCCCATACGCACGCCAAACCGGAGCAACACCACGCAGAATTAGAACGGCTAAAACAGCGTGCAACTTTTCTGCAGTTAGAAAGCCTGCTGAAAAGTGCGGTCAAAAATAACGGCGTTTTTATCAACCAAACTGTATTCCTGAAACTGATTGAGGATTTGAAAGGCTAt                                                                    >  1:335130/1‑251 (MQ=255)                                                                                                                                                                         caaTTTCACTGTTAATTTTGACCGCACTTTCCTCCTCCTTTGCCAATCAGCACAAGGCGACAACGCATAAATCGAATGTTGCCCATACGCACGCCAAACCGGAGCAACACCACGCAGAATTAGAACGGCTAAAACAGCGTGCAACTTTTCTGCAGTTAGAAAGc                                                                                                                                                        >  1:607040/1‑164 (MQ=255)                                                                                                                                                                         caaTTTCACTGTTAATTTTGACCGCACTTTCCTCCTCCTTTGCCAATCAGCACAAGGCGACAACGCATAAATCGAATGTTGCCCATACGCACGCCAAACCGGAGCAACACCACGCAGAATTAGAACGGCTAAAACAGCGTGCAACTTTTCTGCAGTTAGAAAGCCTGCTAAAAAGTGCGGTCAAAAATAACGGCGTTTTTATCAACCAAACTGTATTCCTGAAACTGATTGAGGATTTGAAAGGCTATCCg                                                                 >  1:454018/1‑251 (MQ=255)                                                                                                                                                                                 cTGTTAATTTTGACCGCACTTTCCTCCTCCTTTGCCAATCAGCACAAGGCGACAACGCATAAATCGAATGTTGCCCATACGCACGCCAAACCGGAGCAACACCACGCAGAATTAGAACGGCTAAAACAGCGTGCAACTTTTCTGCAGTTAGAAAGCCTGCTGAAAAGTGCGGTCAAAAATAACGGCGTTTTTATCAACCAAACTGTATTCCTGAAACTGATTGAGGATTTGAAAGGCTATCCGTTGCaaa                                                          >  1:221052/1‑250 (MQ=255)                                                                                                                                                                                        ttttGACCGCACTTTCCTCCTCCTTTGCCAATCAGCACAAGGCGACAACGCATAAATCGAATGTTGCCCATACGCACGCCAAACCGGAGCAACACCACGCAGAATTAGAACGGCTAAAACAGCGTGCAACTTTTCTGCAGTTAGAAAGCCTGCTG‑AAAGTGCGGTCAAAAATAACGGCGTTTTTATCAACCAAACTGTATTCCTGAAACTGATTGAGGATTTGAAAGGCTATCCGTTGCAAACAGATGCCa                                                 <  1:482673/251‑1 (MQ=255)                                                                                                                                                                                           tGACCGCACTTTCCTCCTCCTTTGCCAATCAGCACAAGGCGACAACGCATAAATCGAATGTTGCCCATACGCACGCCAAACCGGAGCAACACCACGCAGAATTAGAACGGCTAAAACAGCGTGCAACTTTTCTGCAGTTAGAAAGCCTGCTGAAAAGTGCGGTCAAAAATAACGGCGTTTTTATCAACCAAACTGTATTCCTGAAACTGATTGAGGATTTGAAAGGCTATCCGTTGCAAACAGATGCCAtc                                               <  1:727600/251‑2 (MQ=255)                                                                                                                                                                                                gCACTTTCCTCCTCCTTTGCCAATCAGCACAAGGCGACAACGCATAAATCGAATGTTGCCCATACGCACGCCAAACCGGAGCAACACCACGCAGAATTAGAACGGCTAAAACAGCGTGCAACTTTTCTGCAGTTAGAAAGCCTGCTGAAAAGTGCGGTCAAAAATAACGGCGTTTTTATCAACCAAACTGTATTCCTGAAACTGATTGAGGATTTGAAAGGCTATCCGTTGCAAACAGATGCCATCGCgg                                           >  1:217560/1‑250 (MQ=255)                                                                                                                                                                                                 cACTTTCCTCCTCCTTTGCCAATCAGCACAAGGCGACAACGCATAAATCGAATGTTGCCCATACGCACGCCAAACCGGAGCAACACCACGCAGAATTAGAACGGCTAAAACAGCGTGCAACTTTTCTGCAGTTAGAAAGCCTGCTGAAAAGTGCGGTCAAAAATAACGGCGTTTTTATCAACCAAACTGTATTCCTGAAACTGATTGAGGATTTGAAAGGc                                                                       >  1:719872/1‑221 (MQ=255)                                                                                                                                                                                                     ttCCTCCTCCTTTGCCAATCAGCACAAGGCGACAACGCATAAATCGAATGTTGCCCATACGCACGCCAAACCGGAGCAACACCACGCAGAATTAGAACGGCTAAAACAGCGTGCAACTTTTCTGCAGTTAGAAAGCCTGCTGAAAAGTGCGGTCAAAAATAACGGCGTTTTTATCAACCAAACTGTATTCCTg                                                                                               >  1:274721/1‑193 (MQ=255)                                                                                                                                                                                                     ttCCTCCTCCTTTGCCAATCAGCACAAGGCGACAACGCATAAATCGAATGTTGCCCATACGCACGCCAAACCGGAGCAACACCACGCAGAATTAGAACGGCTAAAACAGCGTGCAACTTTTCTGCAGTTAGAAAGCCTGCTGAAAAGTGCGGTCAAAAATAACGGCGTTTTTATCAACCAAACTGTATTCCTGAAACTGATTGAGGATTTGAAAgg                                                                        <  1:771382/216‑1 (MQ=255)                                                                                                                                                                                                     ttCCTCCTCCTTTGCCAATCAGCACAAGGCGACAACGCATAAATCGAATGTTGCCCATACGCACGCCAAACCGGAGCAACACCACGCAGAATTAGAACGGCTAAAACAGCGTGCAACTTTTCTGCAGTTAGAAAGCCTGCTGAAAAGTGCGGTCAAAAATAACGGCGTTTTTATCAACCAAACTGTATTCCTGAAACTGATTGAGGATTTGAAAGGCTATcc                                                                  <  1:195621/222‑1 (MQ=255)                                                                                                                                                                                                     ttCCTCCTCCTTTGCCAATCAGCACAAGGCGACAACGCATAAATCGAATGTTGCCCATACGCACGCCAAACCGGAGCAACACCACGCAGAATTAGAACGGCTAAAACAGCGTGCAACTTTTCTGCAGTTAGAAAGCCTGCTGAAAAGTGCGGTCAAAAATAACGGCGTTTTTATCAACCAAACTGTATTCCTGAAACTGATTGAGGATTTGAAAGGCTATCCGTTGCAAACAGATGCCATCGCGGCTTAtt                                     >  1:144686/1‑251 (MQ=255)                                                                                                                                                                                                      tcctcctccTTTGCCAATCAGCACAAGGCGACAACGCATAAATCGAATGTTGCCCATACGCACGCCAAACCGGAGCAACACCACGCAGAATTAGAACGGCTAAAACAGCGTGCAACTTTTCTGCAGTTAGAAAGCCTGCTGAAAAGTGCGGTCAAAAATAACGGCGTTTTTATCAACCAAACTGTATTCCTGAAACTGATTGAGGATTTGAAAGGCTATCCGTTGCAAACAGATGCCATCGCGGCTTAttt                                    <  1:171647/251‑1 (MQ=255)                                                                                                                                                                                                          cctcctTTGCCAATCAGCACAAGGCGACAACGCATAAATCGAATGTTGCCCATACGCACGCCAAACC‑GAGCAACACCACGCAGAATTAGAACGGCTAAAACAGCGTGCAACTTTTCTGCAGTTAGAAAGCCTGCTGAAAAGTGCGGTCAAAAATAACGGCGTTTTTATCAACCAAACTGTATTCCTGAAACTGATTGAGGATTTGAAAGGCTATCCGTTGCAAACAGATGCCATCGCGGCTTATTTCGACg                               <  1:469457/251‑1 (MQ=255)                                                                                                                                                                                                                ttGCCAATCAGCACAAGGCGACAACGCATAAATCGAATGTTGCCCATACGCACGCCAAACCGGAGCAACACCACGCAGAATTAGAACGGCTAAAACAGCGTGCAACTTTTCTGCAGTTAGAAAGCCTGCTGAAAAGTGCGGTCAAAAATAACGGCGTTTTTATCAACCAAACTGTATTCCTGAAACTGATTGAGGATTTGAAAGGCTATCCGTTGCAAACAGATGCCATCGCGGCTTATTTCGACGCCTg                           >  1:372750/1‑250 (MQ=255)                                                                                                                                                                                                                ttGCCAATCAGCACAAGGCGACAACGCATAAATCGAATGTTGCCCATACGCACGCCAAACCGGAGCAACACCACGCAGAATTAGAACGGCTAAAACAGCGTGCAACTTTTCTGCAGTTAGAAAGCCTGCTGAAAAGTGCGGTCAAAAATAACGGCGTTTTTATCAACCAAACTGTATTCCTGAAACTGATTGAGGA‑TTGAAAGGCTATCCGTTGCAAACAGATGCCATCGCGGCTTATTTCGACGCCTGCa                         >  1:218723/1‑251 (MQ=255)                                                                                                                                                                                                                     aaTCAGCACAAGGCGACAACGCATAAATCGAATGTTGCCCATACGCACGCCAAACCGGAGCAACACCACGCAGAATTAGAACGGCTAAAACAGCGTGCAACTTTTCTGCAGTTAGAAAGCCTGCTGAAAAGTGCGGTCAAAAATAACGGCGTTTTTATCAACCAAACTGTATTCCTGAAACTGATTGAGGATTTGAAAGGCTATCCGTTGCAAACAGATGCCATCGCGGCTTATTTCGACGCCTGCATTaa                     >  1:152239/1‑251 (MQ=255)                                                                                                                                                                                                                     aaTCAGCACAAGGCGACAACGCATAAATCGAATGTTGCCCATACGCACGCCAAACCGGAGCAACACCACGCAGAATTAGAACGGCTAAAACAGCGTGCAACTTTTCTGCAGTTAGAAAGCCTGCTGAAAAGTGCGGTCAAAAATAACGGCGTTTTTATCAACCAAACTGTATTCCTGAAACTGATTGAGGATTTGAAAGGCTATCCGTTGCAAACAGATGCCATCGCGGCTTATTTCGACGCCTGCATTaa                     >  1:528166/1‑251 (MQ=255)                                                                                                                                                                                                                          gCACAAGGCGACAACGCATAAATCGAATGTTGCCCATACGCACGCCAAACCGGAGCAACACCACGCAGAATTAGAACGGCTAAAACAGCGTGCAACTTTTCTGCAGTTAGAAAGCCTGCTGAAAAGTGCGGTCAAAAATAACGGCGTTTTTATCAACCAAACTGTATTCCTGAAACTGATTGAGGATTTGAAAGGCTATCCGTTGCAAACAGATGCCATCGCGGCTTATTTCGACGCCTGCATTAAAAGCg                >  1:782783/1‑251 (MQ=255)                                                                                                                                                                                                                          gCACAAGGCGACAACGCATAAATCGAATGTTGCCCATACGCACGCCAAACCGGAGCAACACCACGCAGAATTAGAACGGCTAAAACAGCGTGCAACTTTTCTGCAGTTAGAAAGCCTGCTGAAAAGTGCGGTCAAAAATAACGGCGTTTTTATCAACCAAACTGTATTCCTGAAACTGATTGAGGATTTGAAAGGCTATCCGTTGCAAACAGATGCCATCGCGGCTTATTTCGACGCCTGCATTAAAAGCg                >  1:50823/1‑251 (MQ=255)                                                                                                                                                                                                                           cacaAGGCGACAACGCATAAATCGAATGTTGCCCATACGCACGCCAAACCGGAGCAACACCACGCAGAATTAGAACGGCTAAAACAGCGTGCAACTTTTCTGCAGTTAGAAAGCCTGCTGAAAAGTGCGGTCAAAAATAACGGCGTTTTTATCAACCAAACTGTATTCCTGAAACTGATTGAGGATTTGAAAGGCTATCCGTTGCAAACAGATGCCATCGCGGCTTATTTCGACGCCTGCATTAAAAg                  <  1:208537/248‑1 (MQ=255)                                                                                                                                                                                                                                        cGCATAAATCGAATGTTGCCCATACGCACGCCAAACCGGAGCAACACCACGCAGAATTAGAACGGCTAAAACAGCGTGCAACTTTTCTGCAGTTAGAAAGCCTGCTGAAAAGTGCGGTCAAAAATAACGGCGTTTTTATCAACCAAACTGTATTCCTGTAACTGATTGAGGGTTTGAAAGGc                                                                       >  1:369566/1‑182 (MQ=255)                                                                                                                                                                                                                                        cGCATAAATCGAATGTTGCCCATACGCACGCCAAACCGGAGCAACACCACGCAGAATTAGAACGGCTAAAACAGCGTGCAACTTTTCTGCAGTTAGAAAGCCTGCTGAAAAGTGCGGTCAAAAATAACGGCGTTTTTATCAACCAAACTGTATTCCTGAAACTGATTGAGGa                                                                                 >  1:686348/1‑172 (MQ=255)                                                                                                                                                                                                                                        cGCATAAATCGAATGTTGCCCATACGCACGCCAAACCGGAGCAACACCACGCAGAATTAGAACGGCTAAAACAGCGTGCAACTTTTCTGCAGTTAGAAAGCCTGCTGAAAAGTGCGGTCAAAAATAACGGCGTTTTTATCAACCAAACTGTATTCCTGAAACTGATTGAGGATTTGAAAGGCTATCCGTTGCAAACAGATGCCATCGCGGCTTATTTCGACGCCTGCATTAAAAGCGTAAATCACGAcaca  >  1:242513/1‑251 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                   TCTCCCGACGCATGCTTATATCACGTAATAATATTAATAAATTAAAATACAGCCGTCGGCTTTAACAACGATATTTCAGCTTTCAGCCCATTTTCAAAGGCAGACTTACTATTCGCCTTTCCCATATTCACCACAGGAAAATAACTATGCGATATTTGAAACAAACAACAATTTCACTGTTAATTTTGACCGCACTTTCCTCCTCCTTTGCCAATCAGCACAAGGCGACAACGCATAAAGCGAATGTTGCCCATACGCACGCCAAACCGGAGCAACACCACGCAGAATTAAAACGGCTAAAACAGCGTGCAACTTTTCTGCAGTTAGAAAGCCTGCTGAAAAGTGCGGTCAAAAATAACGGCGTTTTTATCAACCAAACTGTATTCCTGAAACTGATTGAGGATTTGAAAGGCTATCCGTTGCAAACAGATGCCATAGCGGCTTATTTCGACGCCTGCATTAAAAGCGTAAATCACGACACA  >  1/1413302‑1413783

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑