Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,410,952	G→A	L40L (TTG→TTA)	unknown →	Membrane protein involved in the export of O‑antigen and teichoic acid

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,410,952	0	G	A	100.0%	188.6 / NA	71	L40L (TTG→TTA)	unknown	Membrane protein involved in the export of O‑antigen and teichoic acid
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (41/30);  total (41/30)

ACATCATCCTGCGTGAGGAAAACATTGTGTTAAATCAAAATGGAGTTTTTTATGTTTTTATCCCAAAAGGACAGTATAATTAAGCACTTTATTTCACATCAAATTGAGTAGATGAAATGCCAATTAACTTTACCCAAATCATTAAAGATAGCTTCCATTTCATACAAAATGAAAAACAAAGTGTCATTATATTGTCGGCACTCTATTTCTTTGCCAACATCGCCATAGCATTGTTGCAATCGTCAATGTTGCCAAATGAAGTCATTATTTCCTTAACCGAAAAACAAATCTCTCCTGCGATGTCAGAGGGACAAGCTATAGATTTAGTAATCTTCTTTTTTCTGAAACAATTTATCTATATTTTTATCAGTGCATGGTGCTTGGTGAGTATTCACCAAATCAGTCTGCGTCACTTTAATACCTTCCAACACAGCTTCTCCATAACCTTAAAACGTATTTGGGGAGCAATTTTAATTAGC  >  1/1410717‑1411195                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                    aCATCATCCTGCGTGAGGAAAACATTGTGTTAAATCAAAATGGAGTTTTTTATGTTTTTATCTCAAAAGGACAGTATAATTAAGCACTTTATTTCACATCAAATTGAGTAGATGAAATGCCAATTAACTTTACCCAAATCATTAAAGATAGCTTCCATTTCATACAAAATGAAAAACAAAGTGTCATTATATTGTCGGCACTCTATTTCTTTGCCAACATCGCCATAGCATTGTTACAATCGTCAATGTTg                                                                                                                                                                                                                                      >  1:130349/1‑251 (MQ=255) aCATCATCCTGCGTGAGGAAAACATTGTGTTAAATCAAAATGGAGTTTTTTATGTTTTTATCTCAAAAGGACAGTATAATTAAGCACTTTATTTCACATCAAATTGAGTAGATGAAATGCCAATTAACTTTACCCAAATCATTAAAGATAGCTTCCATTTCATACAAAATGAAAAACAAAGTGTCATTATATTGTCGGCACTCTATTTCTTTGCCAACATCGCCATAGCATTGTTACAATCGTCAATGTTg                                                                                                                                                                                                                                      >  1:246653/1‑251 (MQ=255)      atcCTGCGTGAGGAAAACATTGTGTTAAATCAAAATGGAGTTTTTTATGTTTTTATCTCAAAAGGACAGTATAATTAAGCACTTTATTTCACATCAAATTGAGTAGATGAAATGCCAATTAACTTTACCCAAATCATTAAAGATAGCTTCCATTTCATACAAAATGAAAAACAAAGTGTCATTATATTGTCGGCACTCTATTTCTTTGCCAACATCGCCATAGCATTGTTACAATCGTCAATGTTGCCaaa                                                                                                                                                                                                                                 >  1:559307/1‑251 (MQ=255)                           gtgtTAAATCAAAATGGAGTTTTTTATGTTTTTATCTCAAAAGGACAGTATAATTAAGCACTTTATTTCACATCAAATTGAGTAGATGAAATGCCAATTAACTTTACCCAAATCATTAAAGATAGCTTCCATTTCATACAAAATGAAAAACAAAGTGTCATTATATTGTCGGCACTCTATTTCTTTGCCAACATCGCCATAGCATTGTTACAATCGTCAATGTTGCCAAATGAAGTCATTATTTCCTTaa                                                                                                                                                                                                             >  1:560458/1‑250 (MQ=255)                                 aaTCAAAATGGAGTTTTTTATGTTTTTATCTCAAAAGGACAGTATAATTAAGCACTTTATTTCACATCAAATTGAGTAGATGAAATGCCAATTAACTTTACCCAAATCATTAAAGATAGCTTCCATTTCATACAAAATGAAAAACAAAGTGTCATTATATTGTCGGCACTCTATTTCTTTGCCAACATCGCCATAGCATTGTTACAATCGTCAATGTTGCCAAATGAAGTCATTATTTCCTTAACCGaaaa                                                                                                                                                                                                      >  1:187544/1‑251 (MQ=255)                                     aaaaTGGAGTTTTTTATGTTTTTATCTCAAAAGGACAGTATAATTAAGCACTTTATTTCACATCAAATTGAGTAGATGAAATGCCAATTAACTTTACCCAAATCATTAAAGATAGCTTCCATTTCATACAAAATGAAAAACAAAGTGTCATTATATTGTCGGCACTCTATTTCTTTGCCAACATCGCCATAGCATTGTTACAATCGTCAATGTTGCCAAATGAAGTCATTATTTCCTTAACCGAAAAACaa                                                                                                                                                                                                  <  1:757393/251‑1 (MQ=255)                                      aaaTGGAGTTTTTTATGTTTTTATCTCAAAAGGACAGTATAATTAAGCACTTTATTTCACATCAAATTGAGTAGATGAAATGCCAATTAACTTTACCCAAATCATTAAAGATAGCTTCCATTTCATACAAAATGAAAAACAAAGTGTCATTATATTGTCGGCACTCTATTTCTTTGCCAACATCGCCATAGCATTGTTACAATCGTCAATGTTGCCAAATGAAGTCATTATTTCCTTAACCGAAAAACaaa                                                                                                                                                                                                 >  1:459793/1‑251 (MQ=255)                                            aGTTTTTTATGTTTTTATCTCAAAAGGACAGTATAATTAAGCACTTTATTTCACATCAAATTGAGTAGATGAAATGCCAATTAACTTTACCCAAATCATTAAAGATAGCTTCCATTTCATACAAAATGAAAAACAAAGTGTCATTATATTGTCGGCACTCTATTTCTTTGCCAACATCGCCATAGCATTGTTACAATCGTCAATGTTGCCAAATGAAGTCATTATTTCCTTAACCGAAAAACAAAtctct                                                                                                                                                                                            <  1:417093/250‑1 (MQ=255)                                                             tctcAAAAGGACAGTATAATTAAGCACTTTATTTCACATCAAATTGAGTAGATGAAATGCCAATTAACTTTACCCAAATCATTAAAGATAGCTTCCATTTCATACAAAATGAAAAACAAAGTGTCATTATATTGTCGGCACTCTATTTCTTTGCCAACATCGCCATAGCATTGTTACAAt                                                                                                                                                                                                                                                 >  1:272110/4‑180 (MQ=255)                                                                     ggACAGTATAATTAAGCACTTTATTTCACATCAAATTGAGTAGATGAAATGCCAATTAACTTTACCCAAATCATTAAAGATAGCTTCCATTTCATACAAAATGAAAAACAAAGTGTCATTATATTGTCGGCACTCTATTTCTTTGCCAACATCGCCATAGCATTGTTACAATCGTCAATGTTGCCAAATGAAGTCATTATTTCCTTAACCGAAAAACAAATCTCTCCTGCGATGTCAGAGGGACAAGCtat                                                                                                                                                                  >  1:387993/1‑251 (MQ=255)                                                                        cAGTATAATTAAGCACTTTATTTCACATCAAATTGAGTAGATGAAATGCCAATTAACTTTACCCAAATCATTAAAGATAGCTTCCATTTCATACAAAATGAAAAACAAAGTGTCATTATATTGTCGGCACTCTATTTCTTTGCCAACATCGCCATAGCATTGTTACAATCGTCAATGt                                                                                                                                                                                                                                        >  1:380949/1‑178 (MQ=255)                                                                          gTATAATTAAGCACTTTATTTCACATCAAATTGAGTAGATGAAATGCCAATTAACTTTACCCAAATCATTAAAGATAGCTTCCATTTCATACAAAATGAAAAACAAAGTGTCATTATATTGTTGGCACTCTATTTCTTTGCCAACATCGCCATAGCATTGTTACAATCg                                                                                                                                                                                                                                               >  1:530314/1‑169 (MQ=255)                                                                          gTATAATTAAGCACTTTATTTCACATCAAATTGAGTAGATGAAATGCCAATTAACTTTACCCAAATCATTAAAGATAGCTTCCATTTCATACAAAATGAAAAACAAAGTGTCATTATATTGTCGGCACTCTATTTCTTTGCCAACATCGCCATAGCATTGTTACAATCGTCAATGt                                                                                                                                                                                                                                        >  1:337683/1‑176 (MQ=255)                                                                          gTATAATTAAGCACTTTATTTCACATCAAATTGAGTAGATGAAATGCCAATTAACTTTACCCAAATCATTAAAGATAGCTTCCATTTCATACAAAATGAAAAACAAAGTGTCATTATATTGTCGGCACTCTATTTCTTTGCCAACATCGCCATAGCATTGTTACAATCGTCAATGTTGCCAAATGAAg                                                                                                                                                                                                                            <  1:537470/188‑1 (MQ=255)                                                                          gTATAATTAAGCACTTTATTTCACATCAAATTGAGTAGATGAAATGCCAATTAACTTTACCCAAATCATTAAAGATAGCTTCCATTTCATACAAAATGAAAAACAAAGTGTCATTATATTGTCGGCACTCTATTTCTTTGCCAACATCGCCATAGCATTGTTACAATCGTCAATGTTGCCAAATGAAGTCATTATTTCCTTAACCGAAAAACAAATCTCTCCTGCGATGTc                                                                                                                                                                                 >  1:811845/1‑231 (MQ=255)                                                                          gTATAATTAAGCACTTTATTTCACATCAAATTGAGTAGATGAAATGCCAATTAACTTTACCCAAATCATTAAAGATAGCTTCCATTTCATACAAAATGAAAAACAAAGTGTCATTATATTGTCGGCACTCTATTTCTTTGCCAACATCGCCATAGCATTGTTACAATCGTCAATGTTGCCAAATGAAGTCATTATTTCCTTAACCGAAAAACAAATCTCTCCTGCGATGTCAGAGGGACAAGCTATAGAtt                                                                                                                                                             >  1:64430/1‑251 (MQ=255)                                                                          gTATAATTAAGCACTTTATTTCACATCAAATTGAGTAGATGAAATGCCAATTAACTTTACCCAAATCATTAAAGATAGCTTCCATTTCATACAAAATGAAAAACAAAGTGTCATTATATTGTCGGCACTCTATTTCTTTGCCAACATCGCCATAGCATTGTTACAATCGTCAATGTTGCCAAATGAAGTCATTATTTCCTTAACCGAAAAACAAATCTCTCCTGCGATGTCAGAGGGACAAGCTATAGAtt                                                                                                                                                             >  1:33610/1‑251 (MQ=255)                                                                          gTATAATTAAGCACTTTATTTCACATCAAATTGAGTAGATGAAATGCCAATTAACTTTACCCAAATCATTAAAGATAGCTTCCATTTCATACAAAATGAAAAACAAAGTGTCATTATATTGTCGGCACTCTATTTCTTTGCCAACATCGCCATAGCATTGTTACAATCGTCAATGTTGCCAAATGAAGTCATTATTTCCTTAACCGAAAAACAAATCTCTCCTGCGATGTCAGAGGGACAAGCTATAGAt                                                                                                                                                              <  1:889881/250‑1 (MQ=255)                                                                          gTATAATTAAGCACTTTATTTCACATCAAATTGAGTAGATGAAATGCCAATTAACTTTACCCAAATCATTAAAGATAGCTTCCATTTCATACAAAATGAAAAACAAAGTGTCATTATATTGTCGGCACTCTATTTCTTTGCCAACATCGCCATAGCATTGTTACAATCGTCAATGTTGCCAAATGAAGTCATTATTTCCTTAACCGAAAAACAAATCTCTCCTGCGATGTCAGAGGGACAAGCTATAGAt                                                                                                                                                              >  1:654650/1‑250 (MQ=255)                                                                           tataATTAAGCACTTTATTTCACATCAAATTGAGTAGATGAAATGCCAATTAACTTTACCCAAATCATTAAAGATAGCTTCCATTTCATACAAAATGAAAAACAAAGTGTCATTATATTGTCGGCACTCTATTTCTTTGCCAACATCGCCATAGCATTGTTACAATCGTCAATGTTGCCAAATGAAGTCATTATTTCCTTAACCGAAAAACAAATCTCTCCTGCGATGTCAGAGGGACAAGCTATAGAt                                                                                                                                                              <  1:558156/249‑1 (MQ=255)                                                                           tataATTAAGCACTTTATTTCACATCAAATTGAGTAGATGAAATGCCAATTAACTTTACCCAAATCATTAAAGATAGCTTCCATTTCATACAAAATGAAAAACAAAGTGTCATTATATTGTCGGCACTCTATTTCTTTGCCAACATCGCCATAGCATTGTTACAATCGTCAATGTTGCCAAATGAAGTCATTATTTCCTTAACCGAAAAACAAATCTCTCCTGCGATGTCAGAGGGACAAGCTATAGAt                                                                                                                                                              <  1:389145/249‑1 (MQ=255)                                                                              aaTTAAGCACTTTATTTCACATCAAATTGAGTAGATGAAATGCCAATTAACTTTACCCAAATCATTAAAGATAGCTTCCATTTCATACAAAATGAAAAACAAAGTGTCATTATATTGTCGGCACTCTATTTCTTTGCCAACATCGCCATAGCATTGTTACAATCGTCAATGTTGCCAAATGAAGTCATTATTTCCTTAACCGAAAAACAAATCTCTCCTGCGATGTCAGAGGGACAAGCTATAGAt                                                                                                                                                              <  1:574795/246‑1 (MQ=255)                                                                               aTTAAGCACTTTATTTCACATCAAATTGAGTAGATGAAATGCCAATTAACTTTACCCAAATCATTAAAGATAGCTTCCATTTCATACAAAATGAAAAACAAAGTGTCATTATATTGTCGGCACTCTATTTCTTTGCCAACATCGCCATAGCATTGTTACAATCGTCAATGTTGCCAAATGAAGTCATTATTTCCTTAACCGAAAAACAAATCTCTCCTg                                                                                                                                                                                        <  1:490158/219‑1 (MQ=255)                                                                                ttAAGCACTTTATTTCACATCAAATTGAGTAGATGAAATGCCAATTAACTTTACCCAAATCATTAAAGATAGCTTCCATTTCATACAAAATGAAAAACAAAGTGTCATTATATTGTCGGCACTCTATTTCTTTGCCAACATCGCCATAGCATTGTTACAATCGTCAATGTTGCCAAATGAAGTCATTATTTCCTTAACCGAAAAACAAATCTCTCCTGCGATGTCAGAGGGATAAGCTATAGATTTAGTaa                                                                                                                                                       <  1:674138/251‑1 (MQ=255)                                                                                       cTTTATTTCACATCAAATTGAGTAGATGAAATGCCAATTAACTTTACCCAAATCATTAAAGATAGCTTCCATTTCATACAAAATGAAAAACAAAGTGTCATTATATTGTCGGCACTCTATTTCTTTGCCAACATCGCCATAGCATTGTTACAATCGTCAATGTTGCCAAATGAAGTCATTATTTCCTTAACCGAAAAACAAATCTCTCCTGCGATGTCAGAGGGACAAGCTATAGATTTAGTAATcttctt                                                                                                                                                >  1:25983/1‑251 (MQ=255)                                                                                                     aaaTTGAGTAGATGAAATGCCAATTAACTTTACCCAAATCATTAAAGATAGCTTCCATTTCATACAAAATGAAAAACAAAGTGTCATTATATTGTCGGCACTCTATTTCTTTGCCAACATCGCCATAGCATTGTTACAAt                                                                                                                                                                                                                                                 <  1:396727/140‑1 (MQ=255)                                                                                                     aaaTTGAGTAGATGAAATGCCAATTAACTTTACCCAAATCATTAAAGATAGCTTCCATTTCATACAAAATGAAAAACAAAGTGTCATTATATTGTCGGCACTCTATTTCTTTGCCAACATCGCCATAGCATTGTTACAATCGTCAATGTTGCCAAATGAAGTCATTATTTcc                                                                                                                                                                                                                 <  1:222385/172‑1 (MQ=255)                                                                                                     aaaTTGAGTAGATGAAATGCCAATTAACTTTACCCAAATCATTAAAGATAGCTTCCATTTCATACAAAATGAAAAACAAAGTGTCATTATATTGTCGGCACTCTATTTCTTTGCCAACATCGCCATAGCATTGTTACAATCGTCAATGTTGCCAAATGAAGTCATTATTTCCTTAACCGAAAAACAAATCTCTCCTGCGATGTCAGAGGGACaa                                                                                                                                                                       <  1:47525/214‑1 (MQ=255)                                                                                                          gAGTAGATGAAATGCCAATTAACTTTACCCAAATCATTAAAGATAGCTTCCATTTCATACAAAATGAAAAACAAAGTGTCATTATATTGTCGGCACTCTATTTCTTTGCCAACATCGCCATAGCATTGTTACAATCGTCAATGTTGCCAAATGAAGTCATTATTTCCTTAACCGAAAAACAAATCTCTCCTGCGATGTCAGAGGGACAAGCTATAGAt                                                                                                                                                              >  1:560461/1‑218 (MQ=255)                                                                                                           aGTAGATGAAATGCCAATTAACTTTACCCAAATCATTAAAGATAGCTTCCATTTCATACAAAATGAAAAACAAAGTGTCATTATATTGTCGGCACTCTATTTCTTTGCCAACATCGCCATAGCATTGTTACAATCGTCAATGTTGCCAAATGAAGTCATTATTTCCTTAACCGAAAAACAAATCTCTCCTGCGATGTCAGAGGGACAAGCTATAGATTTAGTAATCTTCTTTTTTCTGAAACAATTTATc                                                                                                                             <  1:827484/250‑1 (MQ=255)                                                                                                                aTGAAATGCCAATTAACTTTACCCAAATCATTAAAGATAGCTTCCATTTCATACAAAATGAAAAACAAAGTGTCATTATATTGTCGGCACTCTATTTCTTTGCCAACATCGCCATAGCATTGTTACAATCGTCAATGTTGCCAAATGAAGTCATTATTTc                                                                                                                                                                                                                  >  1:681086/1‑160 (MQ=255)                                                                                                                       gCCAATTAACTTTACCCAAATCATTAAAGATAGCTTCCATTTCATACAAAATGAAAAACAAAGTGTCATTATATTGTCGGCACTCTATTTCTTTGCCAACATCGCCATAGCATTGTTACAATCGTCAATGTTGCCAAATGAAGTCATTATTTCCTTAACCGAAAAACAAATCTCTCCTGCGATGTCAGAGGGACAAGCTATAGATTTAGTAATCTTCTTTTTTCTGAAACAATTTATCTATATTTTTATCa                                                                                                                >  1:209424/1‑251 (MQ=255)                                                                                                                               aCTTTACCCAAATCATTAAAGATAGCTTCCATTTCATACAAAATGAAAAACAAAGTGTCATTATATTGTCGGCACTCTATTTCTTTGCCAACATCGCCATAGCATTGTTACAATCGTCAATGTTGCCAAATGAAGTCATTATTTCCTTAACCGAAAAACAAATCTCTCCTGCGATGTCAGAGGGACAAGCTATAGATTTAGTAATCTTCTTTTTTCTGAAACAATTTATCTATATTTTTATc                                                                                                                 >  1:448882/1‑242 (MQ=255)                                                                                                                                  ttACCCAAATCATTAAAGATAGCTTCCATTTCATACAAAATGAAAAACAAAGTGTCATTATATTGTCGGCACTCTATTTCTTTGCCAACATCGCCATAGCATTGTTACAATCGTCAATGTTGCCAAATGAAGTCATTATTTCCTTAACCGAAAAACAAATCTCTCCTGCGATGTCAGAGGGACAAGCTATAGATTTAGTAATCTTCTTTTTTCTGAAACAATTTATCTATATTTTTATCAGTGCATGGTGc                                                                                                     <  1:852341/251‑1 (MQ=255)                                                                                                                                    aCCCAAATCATTAAAGATAGCTTCCATTTCATACAAAATGAAAAACAAAGTGTCATTATATTGTCGGCACTCTATTTCTTTGCCAACATCGCCATAGCATTGTTACAATCGTCAATGTTGCCAAATGAAGTCATTATTTCCTTAACCGAAAAACAAATCTCTCCTGCGATGTCAGAGGGACAAGCTATAGATTTAGTAATCTTCTTTTTTCTGAAACAATTTATCTATATTTTTATCAGTGCATGGTGCtt                                                                                                   >  1:695973/1‑251 (MQ=255)                                                                                                                                       cAAATCATTAAAGATAGCTTCCATTTCATACAAAATGAAAAACAAAGTGTCATTATATTGTCGGCACTCTATTTCTTTGCCAACATCGCCATAGCATTGTTACAATCGTCAATGTTGCCAAATGAAGTCATTATTTCCTTAACCGAAAAACAAATCTCTCCTGCGATGTCAGAGGGACAAGCTATAGATTTAGTAATCTTCTTTTTTCTGAAACAATTTATCTATATTTTTATCAGTGCATGGTGCTTGGt                                                                                                <  1:659071/251‑1 (MQ=255)                                                                                                                                       cAAATCATTAAAGATAGCTTCCATTTCATACAAAATGAAAAACAAAGTGTCATTATATTGTCGGCACTCTATTTCTTTGCCAACATCGCCATAGCATTGTTACAATCGTCAATGTTGCCAAATGAAGTCATTATTTCCTTAACCGAAAAACAAATCTCTCCTGCGATGTCAGAGGGACAAGCTATAGATTTAGTAATCTTCTTTTTTCTGAAACAATTTATCTATATTTTTATCAGTGCATGGTGCTTGGt                                                                                                <  1:321586/251‑1 (MQ=255)                                                                                                                                          aTCATTAAAGATAGCTTCCATTTCATACAAAATGAAAAACAAAGTGTCATTATATTGTCGGCACTCTATTTCTTTGCCAACATCGCCATAGCATTGTTACAATCGTCAATGTTGCCAAATGAAGTCATTATTTCCTTAACCGAAAAACAAATCTCTcc                                                                                                                                                                                          >  1:303111/1‑158 (MQ=255)                                                                                                                                           tCATTAAAGATAGCTTCCATTTCATACAAAATGAAAAACAAAGTGTCATTATATTGTCGGCACTCTATTTCTTTGCCAACATCGCCATAGCATTGTTACAATCGTCAATGTTGCCAAATGAAGTCATTATTTCCTTAACCGAAAAACAAATCTCTCCTGCGATGTCAGAGGGACAAGCTATAGATTTAGTAATCTTCTTTTTTCTGAAACAATTTATCTATATTTTTATCAGTGCATGGTGCTTGGTGAGt                                                                                            >  1:27836/1‑251 (MQ=255)                                                                                                                                           tCATTAAAGATAGCTTCCATTTCATACAAAATGAAAAACAAAGTGTCATTATATTGTCGGCACTCTATTTCTTTGCCAACATCGCCATAGCATTGTTACAATCGTCAATGTTGCCAAATGAAGTCATTATTTCCTTAACCGAAAAACAAATCTCTCCTGCGATGTCAGAGGGACAAGCTATAGATTTAGTAATCTTCTTTTTTCTGAAACAATTTATCTATATTTTTATCAGTGCATGGTGCTTGGTGAGt                                                                                            >  1:401419/1‑251 (MQ=255)                                                                                                                                             aTTAAAGATAGCTTCCATTTCATACAAAATGAAAAACAAAGTGTCATTATATTGTCGGCACTCTATTTCTTTGCCAACATCGCCATAGCATTGTTACAATCGTCAATGTTGCCAAATGAAGTCATTATTTCCTTAACCGAAAAACAAATCTCTCCTGCGATGTCAGAGGGACAAGCTATAGATTTAGTAATCTTCTTTTTTCTGAAACAATTTATCTATATTTTTATCAGTGCATGGTGCTTGGTGAGTAt                                                                                          <  1:785819/251‑1 (MQ=255)                                                                                                                                             aTTAAAGATAGCTTCCATTTCATACAAAATGAAAAACAAAGTGTCATTATATTGTCGGCACTCTATTTCTTTGCCAACATCGCCATAGCATTGTTACAATCGTCAATGTTGCCAAATGAAGTCATTATTTCCTTAACCGAAAAACAAATCTCTCCTGCGATGTCAGAGGGACAAGCTATAGATTTAGTAATCTTCTTTTTTCTGAAACAATTTATCTATATTTTTATCAGTGCATGGTGCTTGGTGAGTAt                                                                                          <  1:640698/251‑1 (MQ=255)                                                                                                                                             aTTAAAGATAGCTTCCATTTCATACAAAATGAAAAACAAAGTGTCATTATATTGTCGGCACTCTATTTCTTTGCCAACATCGCCATAGCATTGTTACAATCGTCAATGTTGCCAAATGAAGTCATTATTTCCTTAACCGAAAAACAAATCTCTCCTGCGATGTCAGAGGGACAAGCTATAGATTTAGTAATCTTCTTTTTTCTGAAACAATTTATCTATATTTTTATCAGTGCATGGTGCTTGGTGAGTAt                                                                                          >  1:756012/1‑251 (MQ=255)                                                                                                                                                aaaGATAGCTTCCATTTCATACAAAATGAAAAACAAAGTGTCATTATATTGTCGGCACTCTATTTCTTTGCCAACATCGCCATAGCATTGTTACAATCGTCAATGTTGCCAAATGAAg                                                                                                                                                                                                                            <  1:131779/118‑1 (MQ=255)                                                                                                                                                       gCTTCCATTTCATACAAAATGAAAAACAAAGTGTCATTATATTGTCGGCACTCTATTTCTTTGCCAACATCGCCATAGCATTGTTACAATCGTCAATGTTGCCAAATGAAGTCATTATTTCCTTAACCGAAAAACAAATCTCTCCTGCGATGTCAGAGGGACAAGCt                                                                                                                                                                    <  1:324844/167‑1 (MQ=255)                                                                                                                                                        cTTCCATTTCATACAAAATGAAAAACAAAGTGTCATTATATTGTCGGCACTCTATTTCTTTGCCAACATCGCCATAGCATTGTTACAATCGTCAATGTTGCCAAATGAAGTCATTATTTCCTTAACCGAAAAACAAATCTCTCCTGCGATGTCAGAGGGACAAGCTATAGATTTAGTAATCTTCTTTTTTCTGAAACAATTTATCTATATTTTTATCAGTGCATGGTGCTTGGTGAGTATTCACCAAATCa                                                                               >  1:607423/1‑251 (MQ=255)                                                                                                                                                             aTTTCATACAAAATGAAAAACAAAGTGTCATTATATTGTCGGCACTCTATTTCTTTGCCAACATCGCCATAGCATTGTTACAATCGTCAATGTTGCCAAATGAAGTCATTATTTCCTTAACCGAAAAACAAATCTCTCCTGCGATGTCAGAGGGACAAGCTATAGAt                                                                                                                                                              >  1:85817/1‑167 (MQ=255)                                                                                                                                                                         aTGAAAAACAAAGTGTCATTATATTGTCGGCACTCTATTTCTTTGCCAACATCGCCATAGCATTGTTACAATCGTCAATGTTGCCAAATGAAGTCATTATTTCCTTAACCGAAAAACAAATCTCTCCTGCGATGTCAGAGGGACAAGCTATAGATTTAGTAATCTTCTTTTTTCTGAAACAATTTATCTATATTTTTATCAGTGCATGGTGCTTGGTGAGTATTCACCAAATCAGTCTGCGTCACTTTAAt                                                              >  1:49044/1‑251 (MQ=255)                                                                                                                                                                              aaaCAAAGTGTCATTATATTGTCGGCACTCTATTTCTTTGCCAACATCGCCATAGCATTGTTACAATCGTCAATGTTGCCAAATGAAGTCATTATTTCCTTAACCGAAAAACAAATCTCTCCTGCGATGTCAGAGGGACAAGCTATAGATTTAGTAATCTTCTTTTTTc                                                                                                                                           >  1:521346/1‑169 (MQ=255)                                                                                                                                                                                     gtgtCATTATATTGTCGGCACTCTATTTCTTTGCCAACATCGCCATAGCATTGTTACAATCGTCAATGTTGCCAAATGAAGTCATTATTTCCTTAACCGAAAAACAAATCTCTCCTGCGATGTCagag                                                                                                                                                                             >  1:297909/1‑128 (MQ=255)                                                                                                                                                                                     gtgtCATTATATTGTCGGCACTCTATTTCTTTGCCAACATCGCCATAGCATTGTTACAATCGTCAATGTTGCCAAATGAAGTCATTATTTCCTTAACCGAAAAACAAATCTCTCCTGCGATGTCAGAGGGACAAGCTATAGAt                                                                                                                                                              <  1:421884/143‑1 (MQ=255)                                                                                                                                                                                     gtgtCATTATATTGTCGGCACTCTATTTCTTTGCCAACATCGCCATAGCATTGTTACAATCGTCAATGTTGCCAAATGAAGTCATTATTTCCTTAACCGAAAAACAAATCTCTCCTGCGATGTCAGAGGGACAAGCTATAGATTTAGTAATCTTCTTTTTTCTGAAACAATTTATCTATATTTTTATCAGTGCATGGTGCTTGGTGAGTATTCAcc                                                                                     <  1:115930/216‑1 (MQ=255)                                                                                                                                                                                      tgtCATTATATTGTCGGCACTCTATTTCTTTGCCAACATCGCCATAGCATTGTTACAATCGTCAATGTTGCCAGATGAAGTCATTATTTCCTTAACCGAAAAACAAATCTCTCCTGCGATGTCAGAGGGACAAGCTATAGATTTAGTAATCTTCTTTTTTCTGAAACAATTTATCTATATTTTTATCAGTGCATGGTGCTTGGTGAGTATTCACCAAATCAGTCTGCGTCACTTTAATACCTTCCAacac                                                  <  1:188365/250‑1 (MQ=255)                                                                                                                                                                                        tCATTATATTGTCGGCACTCTATTTCTTTGCCAACATCGCCATAGCATTGTTACAATCGTCAATGTTGCCAAATGAAGTCATTATTTCCTTAACCGAAAAACAAATCTCTCCTGCGATGTCag                                                                                                                                                                               >  1:788594/1‑123 (MQ=255)                                                                                                                                                                                        tCATTATATTGTCGGCACTCTATTTCTTTGCCAACATCGCCATAGCATTGTTACAATCGTCAATGTTGCCAAATGAAGTCATTATTTCCTTAACCGAAAAACAAATCTCTCCTGCGATGTCAGAGGGAc                                                                                                                                                                         >  1:772919/1‑129 (MQ=255)                                                                                                                                                                                          aTTATATTGTCGGCACTCTATTTCTTTGCCAACATCGCCATAGCATTGTTACAATCGTCAATGTTGCCAAATGAAGTCATTATTTCCTTAACCGAAAAACAAATCTCTCCTGCGATGTCAGAGGGACAAGCTATAGAt                                                                                                                                                              <  1:572248/138‑1 (MQ=255)                                                                                                                                                                                          aTTATATTGTCGGCACTCTATTTCTTTGCCAACATCGCCATAGCATTGTTACAATCGTCAATGTTGCCAAATGAAGTCATTATTTCCTTAACCGAAAAACAAATCTCTCCTGCGATGTCAGAGGGACAAGCTATAGATTTAGTAATCTTCTTTTTTCTGAAACAATTTATCTATATTTTTATCAGTGCATGGTGCTTGGTGAGTATTCACCAAATCAGTCTGCGTCAc                                                                    >  1:620912/1‑228 (MQ=255)                                                                                                                                                                                          aTTATATTGTCGGCACTCTATTTCTTTGCCAACATCGCCATAGCATTGTTACAATCGTCAATGTTGCCAAATGAAGTCATTATTTCCTTAACCGAAAAACAAATCTCTCCTGCGATGTCAGAGGGACAAGCTATAGATTTAGTAATCTTCTTTTTTCTGAAACAATTTATCTATATTTTTATCAGTGCATGGTGCTTGGTGAGTATTCACCAAATCAGTCTGCGTCACTTTAATACCTTCCAACACAGCtt                                             >  1:731396/1‑251 (MQ=255)                                                                                                                                                                                             atatTGTCGGCACTCTATTTCTTTGCCAACATCGCCATAGCATTGTTACAATCGTCAATGTTGCCAAATGAAGTCATTATTTCCTTAACCGAAAAACAAATCTCTCCTGCGATGTCAGAGGGACAAGCTATAGATTTAGTAATCTTCTTTTTTCTGAAACAATTTATCTATATTTTTATCAGTGCATGGTGCTTGGTGAGTATTCACCAAATCAGTCTGCGTCACTTTAATAc                                                            <  1:820043/233‑1 (MQ=255)                                                                                                                                                                                                 tGTCGGCACTCTATTTCTTTGCCAACATCGCCATAGCATTGTTACAATCGTCAATGTTGCCAAATGAAGTCATTATTTCCTTAACCGAAAAACAAATCTCTCCTGCGATGTCAGAGGGACAAGCTATAGAt                                                                                                                                                              >  1:623133/1‑131 (MQ=255)                                                                                                                                                                                                         ctctATTTCTTTGCCAACATCGCCATAGCATTGTTACAAt                                                                                                                                                                                                                                                 <  1:319184/40‑1 (MQ=255)                                                                                                                                                                                                         ctctATTTCTTTGCCAACATCGCCATAGCATTGTTACAATCGTCAATGTTGCCAAATGAAGTCATTATTTCCTTAACCGAAAAACAAATCTCTCCTGCGATGTCAGAGGGACAAGCTATAGATTTAGTAATCTTCTTTTTTCTGAAACAATTTATCTATATTTTTATCAGTGCATGGTGCTTGGTGAGTATTCACCAAATCAGTCTGCGTCACTTTAATACCTTCCAacac                                                  >  1:168579/1‑231 (MQ=255)                                                                                                                                                                                                         ctctATTTCTTTGCCAACATCGCCATAGCATTGTTACAATCGTCAATGTTGCCAAATGAAGTCATTATTTCCTTAACCGAAAAACAAATCTCTCCTGCGATGTCAGAGGGACAAGCTATAGATTTAGTAATCTTCTTTTTTCTGAAACAATTTATCTATATTTTTATCAGTGCATGGTGCTTGGTGAGTATTCACCAAATCAGTCTGCGTCACTTTAATACCTTCCAACACAGCTTCTCCATAACCTTaaa                              >  1:355565/1‑251 (MQ=255)                                                                                                                                                                                                           ctATTTCTTTGCCAACATCGCCATAGCATTGTTACAATCGTCAATGTTGCCAAATGAAGTCATTATTTCCTTAACCGAAAAACAAATCTCTCCTGCGATGTCAGAgg                                                                                                                                                                            >  1:84323/1‑107 (MQ=255)                                                                                                                                                                                                                 cTTTGCCAACATCGCCATAGCATTGTTACAATCGTCAATGTTGCCAAATGAAGTCATTATTTCCTTAACCGAAAAACAAATCTCTCCTGCGATGTCAGAGGGACAAGCTATAGATTTAGTAATCTTCTTTTTTCTGAAACAATTTATCTATATTTTTATCAGTGCATGGTGCTTGGTg                                                                                               <  1:877284/178‑1 (MQ=255)                                                                                                                                                                                                                             cGCCATAGCATTGTTACAATCGTCAATGTTGCCAAATGAAGTCATTATTTCCTTAACCGAAAAACAAATCTCTCCTGCGATGTCAGAGGGACAAGCTATAGAt                                                                                                                                                              <  1:727522/103‑1 (MQ=255)                                                                                                                                                                                                                               ccATAGCATTGTTACAATCGTCAATGTTGCCAAATGAAGTCATTATTTCCTTAACCGAAAAACAAATCTCTCCTGCGATGTCAGAGGGACAAGCTATAGAt                                                                                                                                                              >  1:190450/1‑101 (MQ=255)                                                                                                                                                                                                                               ccATAGCATTGTTACAATCGTCAATGTTGCCAAATGAAGTCATTATTTCCTTAACCGAAAAACAAATCTCTCCTGCGATGTCAGAGGGACAAGCTATAGATTTAGTAATCTTCTTTTTTCTGAAACAATTTATCTATATTTTTATCAGTGCATGGt                                                                                                       >  1:256384/1‑156 (MQ=255)                                                                                                                                                                                                                               ccATAGCATTGTTACAATCGTCAATGTTGCCAAATGAAGTCATTATTTCCTTAACCGAAAAACAAATCTCTCCTGCGATGTCAGAGGGACAAGCTATAGATTTAGTAATCTTCTTTTTTCTGAAACAATTTATCTATATTTTTATCAGTGCATGGTGCTTGGTGAGTATTCACCAAATCAGTCTGCGTCACTTTAATAc                                                            <  1:565669/199‑1 (MQ=255)                                                                                                                                                                                                                                    gCATTGTTACAATCGTCAATGTTGCCAAATGAAGTCATTATTTCCTTAACCGAAAAACAAATCTCTcc                                                                                                                                                                                          >  1:856835/1‑68 (MQ=255)                                                                                                                                                                                                                                     cATTGTTACAATCGTCAATGTTGCCAAATGAAGTCATTATTTCCTTAACCGAAAAACAAATCTCTCCTGCGATGTCAGAGGGACAAGCTATAGATTTAGTAATCTTCTTTTTTCTGAAACAATTTATCTATATTTTTATCAGTGCATGGTGCTTGGTGAGTATTCACCAAATCAGTCTGCGTCACTTTAATACCTTCCAACACAGCTTCTCCATAACCTTAAAACGTATTTGGGGAGCAATTTTAATTAGc  <  1:841701/251‑1 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                    ACATCATCCTGCGTGAGGAAAACATTGTGTTAAATCAAAATGGAGTTTTTTATGTTTTTATCCCAAAAGGACAGTATAATTAAGCACTTTATTTCACATCAAATTGAGTAGATGAAATGCCAATTAACTTTACCCAAATCATTAAAGATAGCTTCCATTTCATACAAAATGAAAAACAAAGTGTCATTATATTGTCGGCACTCTATTTCTTTGCCAACATCGCCATAGCATTGTTGCAATCGTCAATGTTGCCAAATGAAGTCATTATTTCCTTAACCGAAAAACAAATCTCTCCTGCGATGTCAGAGGGACAAGCTATAGATTTAGTAATCTTCTTTTTTCTGAAACAATTTATCTATATTTTTATCAGTGCATGGTGCTTGGTGAGTATTCACCAAATCAGTCTGCGTCACTTTAATACCTTCCAACACAGCTTCTCCATAACCTTAAAACGTATTTGGGGAGCAATTTTAATTAGC  >  1/1410717‑1411195

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑