Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,410,779	C→T	P45S (CCA→TCA)	unknown →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,410,779	0	C	T	100.0%	133.5 / NA	48	P45S (CCA→TCA)	unknown	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (27/21);  total (27/21)

ACCTAAGATAATTTTGTGCTTTTGCATAATGAACTCCTAAAAATTATTATAACGTTTATCCCTAATAAAAATGTGTTTATTAAGCGCGGTGAAATATACCACTTTATGAGTAATGCAATATGTGATCTCAATCAAATTTTTGGAAATTAGAGAAAATAATTTAGAGAATCTACATCATTTTTACATCATCCTGCGTGAGGAAAACATTGTGTTAAATCAAAATGGAGTTTTTTATGTTTTTATCCCAAAAGGACAGTATAATTAAGCACTTTATTTCACATCAAATTGAGTAGATGAAATGCCAATTAACTTTACCCAAATCATTAAAGATAGCTTCCATTTCATACAAAATGAAAAACAAAGTGTCATTATATTGTCGGCACTCTATTTCTTTGCCAACATCGCCATAGCATTGTTGCAATCGTCAATGTTGCCAAATGAAGTCATTATTTCCTTAACCGAAAAACAAATCTCT  >  1/1410535‑1411009                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                       aCCTAAGATAATTTTGTGCTTTTGCATAATGAACTCCTAAAAATTATTATAACGTTTATCCCTAATAAAAATGTGTTTATTAAGCGCGGTGAAATATACCACTTTATGAGTAATGCAATATGTGATCTCAATCAAATTTTTGGAAATTAGAGAAAATAATTTAGAGAATCTACATCATTTTTACATCATCCTGCGTGAGGAAAACATTGTGTTAAATCAAAATGGAGTTTTTTATGTTTTTATCTCAAAAg                                                                                                                                                                                                                                  >  1:342229/1‑251 (MQ=255)         tAATTTTGTGCTTTTGCATAATGAACTCCTAAAAATTATTATAACGTTTATCCCTAATAAAAATGTGTTTATTAAGCGCGGTGAAATATACCACTTTATGAGTAATGCAATATGTGATCTCAATCAAATTTTTGGAAATTAGAGAAAATAATTTAGAGAATCTACATCATTTTTACATCATCCTGCGTGAGGAAAACATTGTGTTAAATCAAAATGGAGTTTTTTATGTTTTTATCTCAAAAGGACAGtat                                                                                                                                                                                                                          <  1:387161/251‑1 (MQ=255)                        gCATAATGAACTCCTAAAAATTATTATAACGTTTATCCCTAATAAAAATGTGTTTATTAAGCGCGGTGAAATATACCACTTTATGAGTAATGCAATATGTGATCTCAATCAAATTTTTGGAAATTAGAGAAAATAATTTAGAGAATCTACATCATTTTTACATCATCCTGCGTGAGGAAAACATTGTGTTAAATCAAAATGGAGTTTTTTATGTTTTTATCTCAAAAGGAc                                                                                                                                                                                                                               >  1:321457/1‑231 (MQ=255)                                          aaTTATTATAACGTTTATCCCTAATAAAAATGTGTTTATTAAGCGCGGTGAAATATACCACTTTATGAGTAATGCAATATGTGATCTCAATCAAATTTTTGGAAATTAGAGAAAATAATTTAGAGAATCTACATCATTTTTACATCATCCTGCGTGAGGAAAACATTGTGTTAAATCAAAATGGAGTTTTTTATGTTTTTATCTCAAAAGGACAGTATAATTAAGCACTTTATTTCACATCAAAt                                                                                                                                                                                               >  1:583927/1‑245 (MQ=255)                                            ttattaTAACGTTTATCCCTAATAAAAATGTGTTTATTAAGCGCGGTGAAATATACCACTTTATGAGTAATGCAATATGTGATCTCAATCAAATTTTTGGAAATTAGAGAAAATAATTTAGAGAATCTACATCATTTTTACATCATCCTGCGTGAGGAAAACATTGTGTTAAATCAAAATGGAGTTTTTTATGTTTTTATCTCAAAAGGACAGTATAATTa                                                                                                                                                                                                                     >  1:120556/1‑221 (MQ=255)                                                                 ataaAAATGTGTTTATTAAGCGCGGTGAAATATACCACTTTATGAGTAATGCAATATGTGATCTCAATCAAATTTTTGGAAATTAGAGAAAATAATTTAGAGAATCTACATCATTTTTACATCATCCTGCGTGAGGAAAACATTGTGTTAAATCAAAATGGAGTTTTTTATGTTTTTATCTCAAAAgg                                                                                                                                                                                                                                 <  1:220976/188‑1 (MQ=255)                                                                         gtgtTTATTAAGCGCGGTGAAATATACCACTTTATGAGTAATGCAATATGTGATCTCAATCAAATTTTTGGAAATTAGAGAAAATAATTTAGAGAATCTACATCATTTTTACATCATCCTGCGTGAGGAAAACATTGTGTTAAATCAAAATGGAGTTTTTTATGTTTTTATCTCAAAAGGACAGTATAATTAAGCACTTTATTTCACATCAAATTGAGt                                                                                                                                                                                          >  1:68001/1‑219 (MQ=255)                                                                               attaAGCGCGGTGAAATATACCACTTTATGAGTAATGCAATATGTGATCTCAATCAAATTTTTGGAAATTAGAGAAAATAATTTAGAGAATCTACATCATTTTTACATCATCCTGCGTGAGGAAAACATTGTGTTAAATCAAAATGGAGTTTTTTATGTTTTTATCTCAAAAGGACAGTATAATTAAGCACTTTATTTCACATCAAATTGAGTAGATGAAATGCCAATTAACTTTACCCAAATCATTAAAg                                                                                                                                                    >  1:865550/1‑251 (MQ=255)                                                                                           gAAATATACCACTTTATGAGTAATGCAATATGTGATCTCAATCAAATTTTTGGAAATTAGAGAAAATAATTTAGAGAATCTACATCATTTTTACATCATCCTGCGTGAGGAAAACATTGTGTTAAATCAAAATGGAGTTTTTTATGTTTTTATCTCAAAAGGACAGTATAATTAAGCACTTTATTTCACATCAAATTGAGTAGATGAAATGCCAATTAACTTTACCCAAATCATTAAAGATAGCTTCCAtt                                                                                                                                        >  1:775107/1‑251 (MQ=255)                                                                                                      cTTTATGAGTAATGCAATATGTGATCTCAATCAAATTTTTGGAAATTAGAGAAAATAATTTAGAGAATCTACATCATTTTTACATCATCCTGCGTGAGGAAAACATTGTGTTAAATCAAAATGGAGTTTTTTATGTTTTTATCTCAAAAGGACAGTATAATTAAGCACTTTATTTCACATCAAATTGAGTAGATGAAATGCCAATTAACTTTACCCAAATCATTAAAGATAGCTTCCATTTCATACAAAAt                                                                                                                             >  1:315253/1‑251 (MQ=255)                                                                                                      cTTTATGAGTAATGCAATATGTGATCTCAATCAAATTTTTGGAAATTAGAGAAAATAATTTAGAGAATCTACATCATTTTTACATCATCCTGCGTGAGGAAAACATTGTGTTAAATCAAAATGGAGTTTTTTATGTTTTTATCTCAAAAGGACAGTATAATTAAGCACTTTATTTCACATCAAATTGAGTAGATGAAATGCCAATTAACTCTACCCAAATCATTAAAGATAGCTTCCATTTCATACAAAAt                                                                                                                             >  1:119281/1‑251 (MQ=255)                                                                                                              gTAATGCAATATGTGATCTCAATCAAATTTTTGGAAATTAGAGAAAATAATTTAGAGAATCTACATCATTTTTACATCATCCTGCGTGAGGAAAACATTGTGTTAAATCAAAATGGAGTTTTTTATGTTTTTATCTCAAAAGGACAGTATAATTAAGCACTTTATTTCACATCAAATTGAGTAGATGAAATGCCAATTAACTTTACCCAAATCATTAAAGATAGCTTCCATTTCATACAAAATGaa                                                                                                                          <  1:199239/246‑1 (MQ=255)                                                                                                                 aTGCAATATGTGATCTCAATCAAATTTTTGGAAATTAGAGAAAATAATTTAGAGAATCTACATCATTTTTACATCATCCTGCGTGAGGAAAACATTGTGTTAAATCAAAATGGAGTTTTTTATGTTTTTATCTCAAAAGGACAGTATAATTAAGCAc                                                                                                                                                                                                                <  1:783400/157‑1 (MQ=255)                                                                                                                  tGCAATATGTGATCTCAATCAAATTTTTGGAAATTAGAGAAAATAATTTAGAGAATCTACATCATTTTTACATCATCCTGCGTGAGGAAAACATTGTGTTAAATCAAAATGGAGTTTTTTATGTTTTTATCTCAAAAGGACAGTATAATTAAGCACTTTATTTCACATCAAATTGAGTAGATGAAATGCCAATTAACTTTACCCAAATCATTAAAGAt                                                                                                                                                  <  1:166531/218‑1 (MQ=255)                                                                                                                   gCAATATGTGATCTCAATCAAATTTTTGGAAATTAGAGAAAATAATTTAGAGAATCTACATCATTTTTACATCATTCTGCGTGAGGAAAACATTGTGTTAAATCAAAATGGAGTTTTTTATGTTTTTATCTCAAAAGGACAGTATAATTAAGCACTTTATTTCACATCAAATTGAGTAGATGAAATGCCAATTAACTTTACCCAAATCATTAAAGATAGCTTCCATTTCATACAAAATGAAAAACAAAgtg                                                                                                                >  1:740333/1‑251 (MQ=255)                                                                                                                      atatGTGATCTCAATCAAATTTTTGGAAATTAGAGAAAATAATTTAGAGAATCTACATCATTTTTACATCATCCTGCGTGAGGAAAACATTGTGTTAAATCAAAATGGAGTTTTTTATGTTTTTATCTCAAAAGGACAGTATAATTa                                                                                                                                                                                                                     <  1:117729/147‑1 (MQ=255)                                                                                                                      atatGTGATCTCAATCAAATTTTTGGAAATTAGAGAAAATAATTTAGAGAATCTACATCATTTTTACATCATCCTGCGTGAGGAAAACATTGTGTTAAATCAAAATGGAGTTTTTTATGTTTTTATCTCAAAAGGACAGTATAATTAAGCACTTTATTTc                                                                                                                                                                                                        <  1:778676/160‑1 (MQ=255)                                                                                                                       tatGTGATCTCAATCAAATTTTTGGAAATTAGAGAAAATAATTTAGAGAATCTACATCATTTTTACATCATCCTGCGTGAGGAAAACATTGTGTTAAATCAAAATGGAGTTTTTTATGTTTTTATCTCAAAAGGACAGTATAATTa                                                                                                                                                                                                                     >  1:638547/1‑146 (MQ=255)                                                                                                                       tatGTGATCTCAATCAAATTTTTGGAAATTAGAGAAAATAATTTAGAGAATCTACATCATTTTTACATCATCCTGCGTGAGGAAAACATTGTGTTAAATCAAAATGGAGTTTTTTATGTTTTTATCTCAAAAGGACAGTATAATTAAGCACTTTATTTCACATCAAATTGAGTAGATGAAATGCCaa                                                                                                                                                                            >  1:578356/1‑187 (MQ=255)                                                                                                                        atGTGATCTCAATCAAATTTTTGGAAATTAGAGAAAATAATTTAGAGAATCTACATCATTTTTACATCATCCTGCGTGAGGAAAACATTGTGTTAAATCAAAATGGAGTTTTTTATGTTTTTATCTCAAAAGGACAGTATAATTAAGCACTTTATTTcac                                                                                                                                                                                                      <  1:526431/160‑1 (MQ=255)                                                                                                                        atGTGATCTCAATCAAATTTTTGGAAATTAGAGAAAATAATTTAGAGAATCTACATCATTTTTACATCATCCTGCGTGAGGAAAACATTGTGTTAAATCAAAATGGAGTTTTTTATGTTTTTATCTCAAAAGGACAGTATAATTAAGCACTTTATTTCACATCAAATTGAGTAGATg                                                                                                                                                                                     <  1:653820/177‑1 (MQ=255)                                                                                                                        atGTGATCTCAATCAAATTTTTGGAAATTAGAGAAAATAATTTAGAGAATCTACATCATTTTTACATCATCCTGCGTGAGGAAAACATTGTGTTAAATCAAAATGGAGTTTTTTATGTTTTTATCTCAAAAGGACAGTATAATTAAGCACTTTATTTCACATCAAATTGAGTAGATGAAATGCCAATTAACTTTACCCAAATCATTAAAGATAGCTTCCATTTCATACAAAATGAAAAACAAAGTGTCAtt                                                                                                           >  1:712148/1‑251 (MQ=255)                                                                                                                          gtgATCTCAATCAAATTTTTGGAAATTAGAGAAAATAATTTAGAGAATCTACATCATTTTTACATCATCCTGCGTGAGGAAAACATTGTGTTAAATCAAAATGGAGTTTTTTATGTTTTTATCTCAAAAGGACAGTATAATTAAGCACTTTATTTCACATCAAATTGAGTAGATGAAATGCCAATTAACTTTACCCAAAt                                                                                                                                                            >  1:12426/1‑200 (MQ=255)                                                                                                                              tctcAATCAAATTTTTGGAAATTAGAGAAAATAATTTAGAGAATCTACATCATTTTTACATCATCCTGCGTGAGGAAAACATTGTGTTAAATCAAAATGGAGTTTTTTATGTTTTTATCTCAAAAGGACAGTATAATTAAGCACTTTATTTCACATCAAATTGAGTAGATGAAATGCCAATTAACTTTACCCAAATCATTAAAGATAGCTTCCATTTCATACAAAATGAAAAACAAAGTGTCATTATATTg                                                                                                     <  1:843570/251‑1 (MQ=255)                                                                                                                                 caatcaaATTTTTGGAAATTAGAGAAAATAATTTAGAGAATCTACATCATTTTTACATCATCCTGCGTGAGGAAAACATTGTGTTAAATCAAAATGGAGTTTTTTATGTTTTTATCTCAAAAGGACAGTATAATTAAGCACTTTATTTCACATCAAATTGAGTAGATGAAATGCCAATTAACTTTACCCAAATCATTAAAGATAGCTTCCATTTCATACAAAATGAAAAACAAAGTGTCATTATATTGTCg                                                                                                  >  1:679679/1‑251 (MQ=255)                                                                                                                                                aaaTTAGAGAAAATAATTTAGAGAATCTACATCATTTTTACATCATCCTGCGTGAGGAAAACATTGTGTTAAATCAAAATGGAGTTTTTTATGTTTTTATCTCAAAAGGACAGTATAATTAAGCACTTTATTTCACATCAAATTGAGTAGATGAAATGCCAATTAACTTTACCCAAATCATTAAAGATAGCTTCCATTTCATACAAAATGAAAAACAAAGTGTCATTATATTGTCGGCACTCTATTTCtt                                                                                    <  1:601100/250‑1 (MQ=255)                                                                                                                                                        gaAAATAATTTAGAGAATCTACATCATTTTTACATCATCCTGCGTGAGGAAAACATTGTGTTAAATCAAAATGGAGTTTTTTATGTTTTTATCTCAAAAGGACAGTATAATTAAGCACTTTATTTCACATCAAATTGAGTAGATGAAACGCCAATTAACTTTACCCAAATCATTAAAGATAGCTTCCATTTCATACAAAATGAAAAACAAAGTGTCATtata                                                                                                        <  1:23563/222‑1 (MQ=255)                                                                                                                                                               atTTAGAGAATCTACATCATTTTTACATCATCCTGCGTGAGGAAAACATTGTGTTAAATCAAAATGGAGTTTTTTATGTTTTTATCTCAAAAGGACAGTATAATTAAGCACttt                                                                                                                                                                                                             >  1:277465/1‑114 (MQ=255)                                                                                                                                                                   agagAATCTACATCATTTTTACATCATCCTGCGTGAGGAAAACATTGTGTTAAATCAAAATGGAGTTTTTTATGTTTTTATCTCAAAAGGACAGtat                                                                                                                                                                                                                          >  1:213653/1‑97 (MQ=255)                                                                                                                                                                                  tttttACATCATCCTGCGTGAGGAAAACATTGTGTTAAATCAAAATGGAGTTTTTTATGTTTTTATCTCAAAAGGACAGTATAATTa                                                                                                                                                                                                                     <  1:682552/87‑1 (MQ=255)                                                                                                                                                                                  tttttACATCATCCTGCGTGAGGAAAACATTGTGTTAAATCAAAATGGAGTTTTTTATGTTTTTATCTCAAAAGGACAGTATAATTAAGCACTTTATTTCACATCAAATTGAGTAGATGAAATGCCAATTAACTTTACCCAAATCATTAAAGATAGCTTCCATTTCATACAAAATGAAAAACAAAGTGTCATTATATTGTCGGCACTCTATTTc                                                                                      >  1:318885/1‑214 (MQ=255)                                                                                                                                                                                       aCATCATCCTGCGTGAGGAAAACATTGTGTTAAATCAAAATGGAGTTTTTTATGTTTTTATCTCAAAAGGACAGTATAAt                                                                                                                                                                                                                       <  1:66171/80‑1 (MQ=255)                                                                                                                                                                                       aCATCATCCTGCGTGAGGAAAACATTGTGTTAAATCAAAATGGAGTTTTTTATGTTTTTATCTCAAAAGGACAGTATAATTAAGCACTTTATTTc                                                                                                                                                                                                        <  1:764440/95‑1 (MQ=255)                                                                                                                                                                                       aCATCATCCTGCGTGAGGAAAACATTGTGTTAAATCAAAATGGAGTTTTTTATGTTTTTATCTCAAAAGGACAGTATAATTAAGCACTTTATTTCACATCAAAtt                                                                                                                                                                                              >  1:574882/1‑105 (MQ=255)                                                                                                                                                                                       aCATCATCCTGCGTGAGGAAAACATTGTGTTAAATCAAAATGGAGTTTTTTATGTTTTTATCTCAAAAGGACAGTATAATTAAGCACTTTATTTCACATCAAATTGAGTAGATGAAATGCCAATTAACTTTACCCAAATCATTAAAGAt                                                                                                                                                  >  1:211828/1‑149 (MQ=255)                                                                                                                                                                                       aCATCATCCTGCGTGAGGAAAACATTGTGTTAAATCAAAATGGAGTTTTTTATGTTTTTATCTCAAAAGGACAGTATAATTAAGCACTTTATTTCACATCAAATTGAGTAGATGAAATGCCAATTAACTTTACCCAAATCATTAAAGATAGCTTCCATTTCATACAAAATGAAAAACAAAGTGTCATTATATTGTCGGCACTCTATTTCTTTGCCAACATCGCCATAGCATTGTTACAATCGTCAATGTTg                                            >  1:246653/1‑251 (MQ=255)                                                                                                                                                                                       aCATCATCCTGCGTGAGGAAAACATTGTGTTAAATCAAAATGGAGTTTTTTATGTTTTTATCTCAAAAGGACAGTATAATTAAGCACTTTATTTCACATCAAATTGAGTAGATGAAATGCCAATTAACTTTACCCAAATCATTAAAGATAGCTTCCATTTCATACAAAATGAAAAACAAAGTGTCATTATATTGTCGGCACTCTATTTCTTTGCCAACATCGCCATAGCATTGTTACAATCGTCAATGTTg                                            >  1:130349/1‑251 (MQ=255)                                                                                                                                                                                           catcCTGCGTGAGGAAAACATTGTGTTAAATCAAAATGGAGTTTTTTATGTTTTTATCTCAAAAGGACAGTATAATTAAGCACTTTATTTCACATCAAATTGAGTAGATGAAATGCCAATTAACTTTACCCAAATCATTAAAGATAGCTTCCATTTCATACAAAATGAAAAACAAAGTGTCATTATATTGTCGGCACTCTATTTCtt                                                                                    <  1:355709/207‑1 (MQ=255)                                                                                                                                                                                            atcCTGCGTGAGGAAAACATTGTGTTAAATCAAAATGGAGTTTTTTATGTTTTTATCTCAAAAGGACAGTATAATTAAGCACTTTATTTCACATCAAATTGAGTAGATGAAATGCCAATTAACTTTACCCAAATCATTAAAGATAGCTTCCATTTCATACAAAATGAAAAACAAAGTGTCATTATATTGTCGGCACTCTATTTc                                                                                      >  1:237883/1‑204 (MQ=255)                                                                                                                                                                                            atcCTGCGTGAGGAAAACATTGTGTTAAATCAAAATGGAGTTTTTTATGTTTTTATCTCAAAAGGACAGTATAATTAAGCACTTTATTTCACATCAAATTGAGTAGATGAAATGCCAATTAACTTTACCCAAATCATTAAAGATAGCTTCCATTTCATACAAAATGAAAAACAAAGTGTCATTATATTGTCGGCACTCTATTTCTTTGCCAACATCGCCATAGCAt                                                                <  1:787796/226‑1 (MQ=255)                                                                                                                                                                                            atcCTGCGTGAGGAAAACATTGTGTTAAATCAAAATGGAGTTTTTTATGTTTTTATCTCAAAAGGACAGTATAATTAAGCACTTTATTTCACATCAAATTGAGTAGATGAAATGCCAATTAACTTTACCCAAATCATTAAAGATAGCTTCCATTTCATACAAAATGAAAAACAAAGTGTCATTATATTGTCGGCACTCTATTTCTTTGCCAACATCGCCATAGCATTGTTACAATCGTCAATGTTGCCaaa                                       >  1:559307/1‑251 (MQ=255)                                                                                                                                                                                                                 gtgtTAAATCAAAATGGAGTTTTTTATGTTTTTATCTCAAAAGGACAGTATAATTAAGCACTTTATTTCACATCAAATTGAGTAGATGAAATGCCAATTAACTTTACCCAAATCATTAAAGATAGCTTCCATTTCATACAAAATGAAAAACAAAGTGTCATTATATTGTCGGCACTCTATTTc                                                                                      <  1:11107/183‑1 (MQ=255)                                                                                                                                                                                                                 gtgtTAAATCAAAATGGAGTTTTTTATGTTTTTATCTCAAAAGGACAGTATAATTAAGCACTTTATTTCACATCAAATTGAGTAGATGAAATGCCAATTAACTTTACCCAAATCATTAAAGATAGCTTCCATTTCATACAAAATGAAAAACAAAGTGTCATTATATTGTCGGCACTCTATTTCTTTGCCAACATCGCCATAGCATTGTTACAATCGTCAATGTTGCCAAATGAAGTCATTATTTCCTTaa                   >  1:560458/1‑250 (MQ=255)                                                                                                                                                                                                                       aaTCAAAATGGAGTTTTTTATGTTTTTATCTCAAAAGGACAGTATAATTAAGCACTTTATTTCACATCAAATTGAGTAGATGAAATGCCAATTAACTTTACCCAAATCATTAAAGATAGCTTCCATTTCATACAAAATGAAAAACAAAGTGTCATTATATTGTCGGCACTCTATTTCTTTGCCAACATCGCCATAGCATTGTTACAATCGTCAATGTTGCCAAATGAAGTCATTATTTCCTTAACCGaaaa            >  1:187544/1‑251 (MQ=255)                                                                                                                                                                                                                         tCAAAATGGAGTTTTTTATGTTTTTATCTCAAAAGGACAGTATAATTAAGCACTTTATTTCACATCAAATTGAGTAGATGAAATGCCAATTAACTTTACCCAAATCATTAAAGATAGCTTCCATTTCATACAAAATGAAAAACAAAGTGTCATTATATTGTCGGCACTCTATTTCTTTg                                                                                  <  1:594442/179‑1 (MQ=255)                                                                                                                                                                                                                           aaaaTGGAGTTTTTTATGTTTTTATCTCAAAAGGACAGTATAATTAAGCACTTTATTTCACATCAAATTGAGTAGATGAAATGCCAATTAACTTTACCCAAATCATTAAAGATAGCTTCCATTTCATACAAAATGAAAAACAAAGTGTCATTATATTGTCGGCACTCTATTTCTTTGCCAACATCGCCATAGCATTGTTACAATCGTCAATGTTGCCAAATGAAGTCATTATTTCCTTAACCGAAAAACaa        <  1:757393/251‑1 (MQ=255)                                                                                                                                                                                                                            aaaTGGAGTTTTTTATGTTTTTATCTCAAAAGGACAGTATAATTAAGCACTTTATTTCACATCAAATTGAGTAGATGAAATGCCAATTAACTTTACCCAAATCATTAAAGATAGCTTCCATTTCATACAAAATGAAAAACAAAGTGTCATTATATTGTCGGCACTCTATTTCTTTGCCAACATCGCCATAGCATTGTTACAATCGTCAATGTTGCCAAATGAAGTCATTATTTCCTTAACCGAAAAACaaa       >  1:459793/1‑251 (MQ=255)                                                                                                                                                                                                                                  aGTTTTTTATGTTTTTATCTCAAAAGGACAGTATAATTAAGCACTTTATTTCACATCAAATTGAGTAGATGAAATGCCAATTAACTTTACCCAAATCATTAAAGATAGCTTCCATTTCATACAAAATGAAAAACAAAGTGTCATTATATTGTCGGCACTCTATTTCTTTGCCAACATCGCCATAGCATTGTTACAATCGTCAATGTTGCCAAATGAAGTCATTATTTCCTTAACCGAAAAACAAAtctct  <  1:417093/250‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                       ACCTAAGATAATTTTGTGCTTTTGCATAATGAACTCCTAAAAATTATTATAACGTTTATCCCTAATAAAAATGTGTTTATTAAGCGCGGTGAAATATACCACTTTATGAGTAATGCAATATGTGATCTCAATCAAATTTTTGGAAATTAGAGAAAATAATTTAGAGAATCTACATCATTTTTACATCATCCTGCGTGAGGAAAACATTGTGTTAAATCAAAATGGAGTTTTTTATGTTTTTATCCCAAAAGGACAGTATAATTAAGCACTTTATTTCACATCAAATTGAGTAGATGAAATGCCAATTAACTTTACCCAAATCATTAAAGATAGCTTCCATTTCATACAAAATGAAAAACAAAGTGTCATTATATTGTCGGCACTCTATTTCTTTGCCAACATCGCCATAGCATTGTTGCAATCGTCAATGTTGCCAAATGAAGTCATTATTTCCTTAACCGAAAAACAAATCTCT  >  1/1410535‑1411009

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑