Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,395,448	A→G	*116W (TGA→TGG)	unknown →	Galactose/methyl galactoside ABC transporter, ATP‑binding protein MglA (EC 3.6.3.17)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,395,448	0	A	G	100.0%	202.2 / NA	73	*116W (TGA→TGG)	unknown	Galactose/methyl galactoside ABC transporter, ATP‑binding protein MglA (EC 3.6.3.17)
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (40/33);  total (40/33)

CGTCCGTTCACATTCTGTACACGCATTGATGGGGGAAAACGGTGCGGGTAAATCCACACTGCTAAAATGTTTATTTGGTATTTATGCTAAAGATGAAGGCGATATTTTATTTTTAGGCAAGCCCGTTAACTTTAAAACCTCAAAAGAAGCCCTGGAAAACGGCATTTCCATGGTGCACCAGGAATTGAATCTGGTGCGTCAGACCAGCGTAATGGATAACCTGTGACTCGGACGTTATCCGCTGAAAGGACCGTTTGTGGATCACGCCAAAATCTATCGTGATACCAAAGCCATTTTTGACGAGTTGGATATTGATATTGATCCGAAAGAAAAAGTGGCGAAACTTTCCGTTTCTCAAATGCAGATGATCGAAATTGCGAAAGCGTTTTCTTATAACGCCAAAATCGTGATTATGGATGAACCGACATCCTCCCTTTCCGAAAAAGAAGTGGAACATCTGTTTAAAATC  >  1/1395223‑1395691                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                    cgtccgtTCACATTCTGTACACGCATTGATGGGGGAAAACGGTGCGGGTAAATCCACACTGCTAAAATGTTTATTTGGTATTTATGCTAAAGATGAAGGCGATATTTTATTTTTAGGCAAGCCCGTTAACTTTAAAACCTCAAAATAAGCCCTGGAAAACGGCATTTCCATGGTGCACCAGGAATTGAATCTGGTGCGTCAGACCAGCGTAATGGATAACCTGTGGCTCGGACGTTATCCGCTGAAAGGAc                                                                                                                                                                                                                            <  1:10355/251‑1 (MQ=255)   tccgtTCACATTCTGTACACGCATTGATGGGGGAAAACGGTGCGGGTAAATCCACACTGCTAAAATGTTTATTTGGTATTTATGCTAAAGATGAAGGCGATATTTTATTTTTAGGCAAGCCCGTTAACTTTAAAACCTCAAAATAAGCCCTGGAAAACGGCATTTCCATGGTGCACCAGGAATTGAATCTGGTGCGTCAGACCAGCGTAATGGATAACCTGTGGCTCGGACGTTATCCGCTGAAAGGACCg                                                                                                                                                                                                                          >  1:247499/1‑251 (MQ=255)       ttCACATTCTGTACACGCATTGATGGGGGAAAACGGTGCGGGTAAATCCACACTGCTAAAATGTTTATTTGGTATTTATGCTAAAGATGAAGGCGATATTTTATTTTTAGGCAAGCCCGTTAACTTTAAAACCTCAAAATAAGCCCTGGAAAACGGCATTTCCATGGTGCACCAGGAATTGAATCTGGTGCGTCAGACCAGCGTAATGGATAACCTGTGGCTCGGACGTTATCCGCTGAAAgg                                                                                                                                                                                                                              <  1:130934/243‑1 (MQ=255)       ttCACATTCTGTACACGCATTGATGGGGGAAAACGGTGCGGGTAAATCCACACTGCTAAAATGTTTATTTGGTATTTATGCTAAAGATGAAGGCGATATTTTATTTTTAGGCAAGCCCGTTAACTTTAAAACCTCAAAATAAGCCCTGGAAAACGGCATTTCCATGGTGCACCAGGAATTGAATCTGGTGCGTCAGACCAGCGTAATGGATAACCTGTGGCTCGGACGTTATCCGCTGAAAGGACCGTTtg                                                                                                                                                                                                                      >  1:266126/1‑251 (MQ=255)           caTTCTGTACACGCATTGATGGGGGAAAACGGTGCGGGTAAATCCACACTGCTAAAATGTTTATTTGGTATTTATGCTAAAGATGAAGGCGATATTTTATTTTTAGGCAAGCCCGTTAACTTTAAAACCTCAAAATAAGCCCTGGAAAACGGCATTTCCATGGTGCACCAGGAATTGAATCTGGTGCGTCAGACCAGCGTAATGGATAACCTGTGGCTCGGACGTTATCCGCTGAAAGGACCGTTTGTGGa                                                                                                                                                                                                                  <  1:245711/251‑1 (MQ=255)            aTTCTGTACACGCATTGATGGGGGAAAACGGTGCGGGTAAATCCACACTGCTAAAATGTTTATTTGGTATTTATGCTAAAGATGAAGGCGATATTTTATTTTTAGGCAAGCCCGTTAACTTTAAAACCTCAAAATAAGCCCTGGAAAACGGCATTTCCATGGTGCACCAGGAATTGAATCTGGTGCGTCAGACCAGCGTAATGGATAACCTGTGGCTCGGACGTTATCCGCTGAAAGGACCGTTTGTGGAt                                                                                                                                                                                                                 <  1:166306/251‑1 (MQ=255)             ttCTGTACACGCATTGATGGGGGAAAACGGTGCGGGTAAATCCACACTGCTAAAATGTTTATTTGGTATTTATGCTAAAGATGAAGGCGATATTTTATTTTTAGGCAAGCCCGTTAACTTTAAAACCTCAAAATAAGCCCTGGAAAACGGCATTTCCATGGTGCACCAGGAATTGAATCTGGTGCGTCAGACCAGCGTAATGGATAACCTGTGGCTCGGACGTTATCCGCTGAAAGGACCGTTTGTGGAt                                                                                                                                                                                                                 >  1:604217/1‑250 (MQ=255)               cTGTACACGCATTGATGGGGGAAAACGGTGCGGGTAAATCCACACTGCTAAAATGTTTATTTGGTATTTATGCTAAAGATGAAGGCGATATTTTATTTTTAGGCAAGCCCGTTAACTTTAAAACCTCAAAATAAGCCCTGGAAAACGGCATTTCCATGGTGCACCAGGAATTGAATCTGGTGCGTCAGACCAGCGTAATGGATAACCTGTGGCTCGGACGTTATCCGCTGAAAGGACCGTTTGTGGATCa                                                                                                                                                                                                               >  1:665889/1‑250 (MQ=255)               cTGTACACGCATTGATGGGGGAAAACGGTGCGGGTAAATCCACACTGCTAAAATGTTTATTTGGTATTTATGCTAAAGATGAAGGCGATATTTTATTTTTAGGCAAGCCCGTTAACTTTAAAACCTCAAAATAAGCCCTGGAAAACGGCATTTCCATGGTGCACCAGGAATTGAATCTGGTGCGTCAGACCAGCGTAATGGATAACCTGTGGCTCGGACGGTATCCGCTGAAAGGACCGTTTGTGGATCa                                                                                                                                                                                                               >  1:707778/1‑250 (MQ=255)                 gTACACGCATTGATGGGGGAAAACGGTGCGGGTAAATCCACACTGCTAAAATGTTTATTTGGTATTTATGCTAAAGATGAAGGCGATATTTTATTTTTAGGCAAGCCCGTTAACTTTAAAACCTCAAAATAAGCCCTGGAAAACGGCATTTCCATGGTGCACCAGGAATTGAATCTGGTGCGTCAGACCAGCGTAATGGATAACCTGTGGCTCGGACGTTATCCGCTGAAAGGACCGTTTGTGGATCACg                                                                                                                                                                                                             >  1:320052/1‑250 (MQ=255)                 gTACACGCATTGATGGGGGAAAACGGTGCGGGTAAATCCACACTGCTAAAATGTTTATTTGGTATTTATGCTAAAGATGAAGGCGATATTTTATTTTTAGGCAAGCCCGTTAACTTTAAAACCTCAAAATAAGCCCTGGAAAACGGCATTTCCATGGTGCACCAGGAATTGAATCTGGTGCGTCAGACCAGCGTAATGGATAACCTGTGGCTCGGACGTTATCCGCTGAAAGGACCGTTTGTGGATCACg                                                                                                                                                                                                             >  1:91644/1‑250 (MQ=255)                      cGCATTGATGGGGGAAAACGGTGCGGGTAAATCCACACTGCTAAAATGTTTATTTGGTATTTATGCTAAAGATGAAGGCGATATTTTATTTTTAGGCAAGCCCGTTAACTTTAAAACCTCAAAATAAGCCCTGGAAAACGGCATTTCCATGGTGCACCAGGAATTGAATCTGGTGCGTCAGACCAGCGTAATGGATAACCTGTGGCTCGGACGTTATCCGCTGAAAGGACCGTTTGTGGATCACGCCaaaa                                                                                                                                                                                                       >  1:428224/1‑251 (MQ=255)                        cATTGATGGGGGAAAACGGTGCGGGTAAATCCACACTGCTAAAATGTTTATTTGGTATTTATGCTAAAGATGAAGGCGATATTTTATTTTTAGGCAAGCCCGTTAACTTTAAAACCTCAAAATAAGCCCTGGAAAACGGCATTTCCATGGTGCACCAGGAATTGAATCTGGTGCGTCAGACCAGCGTAATGGATAACCTGTGGCTCGGACGTTATCCGCTGAAAGGACCGTTTGTGGAt                                                                                                                                                                                                                 <  1:274963/239‑1 (MQ=255)                           tGATGGGGGAAAACGGTGCGGGTAAATCCACACTGCTAAAATGTTTATTTGGTATTTATGCTAAAGATGAAGGCGATATTTTATTTTTAGGCAAGCCCGTTAACTTTAAAACCTCAAAATAAGCCCTGGAAAACGGCATTTCCATGGTGCACCAGGAATTGAATCTGGTGCGTCAGACCAGCGTAATGGATAACCTGTGGCTCGGACGTTATCCGCTGAAAGGACCGTTTGTGGATCACGCCAAAATCTAt                                                                                                                                                                                                  <  1:640440/251‑1 (MQ=255)                              tGGGGGAAAACGGTGCGGGTAAATCCACACTGCTAAAATGTTTATTTGGTATTTATGCTAAAGATGAAGGCGATATTTTATTTTTAGGCAAGCCCGTTAACTTTAAAACCTCAAAATAAGCCCTGGAAAACGGCATTTCCATGGTGCACCAGGAATTGAATCTGGTGCGTCAGACCAGCGTAATGGATAACCTGTGGCTCGGACGTTATCCGCTGAAAGGACCGTTTGTGGAt                                                                                                                                                                                                                 >  1:418156/1‑233 (MQ=255)                                               ggTAAATCCACACTGCTAAAATGTTTATTTGGTATTTATGCTAAAGATGAAGGCGATATTTTATTTTTAGGCAAGCCCGTTAACTTTAAAACCTCAAAATAAGCCCTGGAAAACGGCATTTCCATGGTGCACCAGGAATTGAATCTGGTGCGTCAGACCAGCGTAATGGATAACCTGTGGCTCGGACGTTATCCGCTGAAAGGACCGTTTGTGGATCACGCCAAAATCTATCg                                                                                                                                                                                                >  1:316916/1‑233 (MQ=255)                                                           cTGCTAAAATGTTTATTTGGTATTTATGCTAAAGATGAAGGCGATATTTTATTTTTAGGCAAGCCCGTTAACTTTAAAACCTCAAAATAAGCCCTGGAAAACGGCATTTCCATGGTGCACCAGGAATTGAATCTGGTGCGTCAGACCAGCGTAATGGATAACCTGTGGCTCGGACGTTATCCGCTGAAAGGACCGTTTGTGGATCACGCCAAAATCTATCGTGATACCAAAGCCATTTTTGACGAGTTGga                                                                                                                                                                  >  1:696233/1‑251 (MQ=255)                                                           cTGCTAAAATGTTTATTTGGTATTTATGCTAAAGATGAAGGCGATATTTTATTTTTAGGCAAGCCCGTTAACTTTAAAACCTCAAAATAAGCCCTGGAAAACGGCATTTCCATGGTGCACCAGGAATTGAATCTGGTGCGTCAGACCAGCGTAATGGATAACCTGTGGCTCGGACGTTATCCGCTGAAAGGACCGTTTGTGGATCACGCCAAAATCTATCGTGATACCAAAGCCATTTTTGACGAGTTGga                                                                                                                                                                  >  1:439088/1‑251 (MQ=255)                                                           cTGCTAAAATGTTTATTTGGTATTTATGCTAAAGATGAAGGCGATATTTTATTTTTAGGCAAGCCCGTTAACTTTAAAACCTCAAAATAAGCCCTGGAAAACGGCATTTCCATGGTGCACCAGGAATTGAATCTGGTGCGTCAGACCAGCGTAATGGATAACCTGTGGCTCGGACGTTATCCGCTGAAAGGACCGTTTGTGGATCACGCCAAAATCTATCGTGATACCAAAGCCATTTTTGACGAGTTGga                                                                                                                                                                  >  1:673354/1‑251 (MQ=255)                                                           cTGCTAAAATGTTTATTTGGTATTTATGCTAAAGATGAAGGCGATATTTTATTTTTAGGCAAGCCCGTTAACTTTAAAACCTCAAAA‑TAGCCCTGGAAAACGGCATTTCCATGGTGCACCAGGAATTGAATCTGGTGCGTCAGACCAGCGTAATGGATAACCTGTGGCTCGGACGTTATCCGCTGAAAGGACCGTTTGTGGATCACGCCAAAATCTATCGTGATACCAAAGCCATTTTTGACGAGTTGgat                                                                                                                                                                 >  1:183564/1‑251 (MQ=255)                                                             gCTAAAATGTTTATTTGGTATTTATGCTAAAGATGAAGGCGATATTTTATTTTTAGGCAAGCCCGTTAACTTTAAAACCTCAAAATAAGCCCTGGAAAACGGCATTTCCATGGTGCACCAGGAATTGAATCTGGTGCGTCAGACCAGCGTAATGGATAACCTGTGGCTCGGACGTTATCCGCTGAAAGGACCGTTTGTGGATCACGCCAAAATCTATCGt                                                                                                                                                                                               >  1:487226/1‑220 (MQ=255)                                                             gCTAAAATGTTTATTTGGTATTTATGCTAAAGATGAAGGCGATATTTTATTTTTAGGCAAGCCCGTTAACTTTAAAACCTCAAAATAAGCCCTGGAAAACGGCATTTCCATGGTGCACCAGGAATTGAATCTGGTGCGTCAGACCAGCGTAATGGATAACCTGTGGCTCGGACGTTATCCGCTGAAAGGACCGTTTGTGGATCACGCCAAAATCTATCGTGATACCAAAGCCATTTTTGACGAGTTGgata                                                                                                                                                                >  1:687622/1‑251 (MQ=255)                                                              cTAAAATGTTTATTTGGTATTTATGCTAAAGATGAAGGCGATATTTTATTTTTAGGCAAGCCCGTTAACTTTAAAACCTCAAAATAAGCCCTGGAAAACGGCATTTCCATGGTGCACCAGGAATTGAATCTGGTGCGTCAGACCAGCGTAATGGATAACCTGTGGCTCGGACGTTATCCGCTGAAAGGACCGTTTGTGGATCACGCCAAAATCTATCGTGATACCAAAGCCATTTTTGACGAGTTGgatat                                                                                                                                                               <  1:356777/251‑1 (MQ=255)                                                                 aaaTGTTTATTTGGTATTTATGCTAAAGATGAAGGCGATATTTTATTTTTAGGCAAGCCCGTTAACTTTAAAACCTCAAAATAAGCCCTGGAAAACGGCATTTCCATGGTGCACCAGGAATTGAATCTGGTGCGTCAGACCAGCGTAATGGATAACCTGTGGCTCGGACGTTATCCGCTGAAAGGACCGTTTGTGGATCACGCCAAAATCTATCGTGATACCAAAGCCATTTTTGACGAGTTGgatattga                                                                                                                                                            <  1:89673/251‑1 (MQ=255)                                                                    tGTTTATTTGGTATTTATGCTAAAGATGAAGGCGATATTTTATTTTTAGGCAAGCCCGTTAACTTTAAAACCTCAAAATAAGCCCTGGAAAACGGCATTTCCATGGTGCACCAGGAATTGAATCTGGTGCGTCAGACCAGCGTAATGGATAACCTGTGGCTCGGACGTTATCCGCTGAAAGGACCGTTTGTGGATCACGCCAAAATCTATCGTGATACCAAAGCCATTTTTGACGAGTTGgatat                                                                                                                                                               >  1:306176/1‑245 (MQ=255)                                                                    tGTTTATTTGGTATTTATGCTAAAGATGAAGGCGATATTTTATTTTTAGGCAAGCCCGTTAACTTTAAAACCTCAAAATAAGCCCTGGAAAACGGCATTTCCATGGTGCACCAGGAATTGAATCTGGTGCGTCAGACCAGCGTAATGGATAACCTGTGGCTCGGACGTTATCCGCTGAAAGGACCGTTTGTGGATCACGCCAAAATCTATCGAGATACCAAAGCCATTTTTGACGAGTTGgatattgatat                                                                                                                                                         >  1:461412/1‑251 (MQ=255)                                                                         aTTTGGTATTTATGCTAAAGATGAAGGCGATATTTTATTTTTAGGCAAGCCCGTTAACTTTAAAACCTCAAAATAAGCCCTGGAAAACGGCATTTCCATGGTGCACCAGGAATTGAATCTGGTGCGTCAGACCAGCGTAATGGATAACCTGTGGCTCGGACGTTATCCGCTGAAAGGACCGTTTGTGGATCACGCCAAAATCTATCGTGATACCAAAGCCATTTTTGACGAGTTGGATATTGATATTGATc                                                                                                                                                    <  1:314412/251‑1 (MQ=255)                                                                              gTATTTATGCTAAAGATGAAGGCGATATTTTATTTTTAGGCAAGCCCGTTAACTTTAAAACCTCAAAATAAGCCCTGGAAAACGGCATTTCCATGGTGCACCAGGAATTGAATCTGGTGCGTCAGACCAGCGTAATGGATAACCTGTGGCTCGGACGTTATCCGCTGAAAGGACCGTTTGTGGATCACGCCAAAATCTATCGTGATACCAAAGcc                                                                                                                                                                                   >  1:20855/1‑215 (MQ=255)                                                                               tATTTATGCTAAAGATGAAGGCGATATTTTATTTTTAGGCAAGCCCGTTAACTTTAAAACCTCAAAATAAGCCCTGGAAAACGGCATTTCCATGGTGCACCAGGAATTGAATCTGGTGCGTCAGACCAGCGTAATGGATAACCTGTGGCTCGGACGTTATCCGCTGAAAGGACCGTTTGTGGATCACGCCAAAATCTATCGTGATACCAAAGCCATTTTTGACGAGTTGGATATTGATATTGATCCgaaag                                                                                                                                              <  1:832760/251‑1 (MQ=255)                                                                                         aaaGATGAAGGCGATATTTTATTTTTAGGCAAGCCCGTTAACTTTAAAACCTCAAAATAAGCCCTGGAAAACGGCATTTCCATGGTGCACCAGGAATTGAATCTGGTGCGTCAGACCAGCGTAATGGATAACCTGTGGCTCGGACGTTATCCGCTGAAAGGACCGTTTGTGGATCACGCCAAAATCTATCGTGATACCAAAGCCATTTTTGACGAGTTGGATATTGATATTGATc                                                                                                                                                    >  1:747702/1‑235 (MQ=255)                                                                                                  ggCGATATTTTATTTTTAGGCAAGCCCGTTAACTTTAAAACCTCAAAATAAGCCCTGGAAAACGGCATTTCCATGGTGCACCAGGAATTGAATCTGGTGCGTCAGACCAGCGTAATGGATAACCTGTGGCTCGGACGTTATCCGCTGAAAGGACCGTTTGTGGATCACGCCAAAATCTATCGTGATACCAAAGCCATTTTTGACGAGTTGgatat                                                                                                                                                               <  1:346444/215‑1 (MQ=255)                                                                                                      atatTTTATTTTTAGGCAAGCCCGTTAACTTTAAAACCTCAAAATAAGCCCTGGAAAACGGCATTTCCATGGTGCACCAGGAATTGAATCTGGTGCGTCAGACCAGCGTAATGGATAACCTGTGGCTCGGACGTTATCCGCTGAAAGGACCGTTTGTGGATCACGCCAAAATCTATCGTGATACCAAAGCCATTTTTGACGAGTTGGATATTGATATTGATc                                                                                                                                                    <  1:210922/222‑1 (MQ=255)                                                                                                                tttAGGCAAGCCCGTTAACTTTAAAACCTCAAAATAAGCCCTGGAAAACGGCATTTCCATGGTGCACCAGGAATTGAATCTGGTGCGTCAGACCAGCGTAATGGATAACCTGTGGCTCGGACGTTATCCGCTGAAAGGACCGTTTGTGGATCACGCCAAAATCTATCGTGATACCAAAGCCATTTTTGACGAGTTGGATATTGATATTGATCCGAAAGAAAAAGTGGCGAAACTTTCCGTTTCTCAAATGc                                                                                                             <  1:614891/251‑1 (MQ=255)                                                                                                                   aGGCAAGCCCGTTAACTTTAAAACCTCAAAATAAGCCCTGGAAAACGGCATTTCCATGGTGCACCAGGAATTGAATCTGGTGCGTCAGACCAGCGTAATGGATAACCTGTGGCTCGGACGTTATCCGCTGAAAGGACCGTTTGTGGATCACGCC‑AAATCTATCGTGATACCAAAGCCATTTTTGACGAGTTGGATATTGATATTGATCCGAAAGAAAAAGTGGCGAAACTTTCCGTTTCTCAAATGCAgat                                                                                                         >  1:374341/1‑251 (MQ=255)                                                                                                                         gCCCGTTAACTTTAAAACCTCAAAATAAGCCCTTGAAAACGGCATTTCCATGGTGCACCAGGAATTGAATCTGGTGCGTCAGACCAGCGTAATGGATAGCCTGTGGCTCGGACGTTAGCCGCTGAAAGGACCGTTTGTGGATCACGCCAAAATCTATCGTGATACCAAAGCCATTTTTGACGAGTTGgatattga                                                                                                                                                            <  1:95305/195‑1 (MQ=255)                                                                                                                          cccGTTAACTTTAAAACCTCAAAATAAGCCCTGGAAAACGGCATTTCCATGGTGCACCAGGAATTGAATCTGGTGCGTCAGACCAGCGTAATGGATAACCTGTGGCTCGGACGTTATCCGCTGAAAGGACCGTTTGTGGATCACGCCAAAATCTATCGTGATACCAAAGcc                                                                                                                                                                                   <  1:459439/171‑1 (MQ=255)                                                                                                                          cccGTTAACTTTAAAACCTCAAAATAAGCCCTGGAAAACGGCATTTCCATGGTGCACCAGGAATTGAATCTGGTGCGTCAGACCAGCGTAATGGATAACCTGTGGCTCGGACGTTATCCGCTGAAAGGACCGTTTGTGGATCACGCCAAAATCTATCGTGATACCAAAGCCATTTTTGACGAGTTGGATATTGATATTGATCCGAAAGAAAAAGTGGCGAAACTTTCCGTTTCTCAAATGCAgatg                                                                                                        >  1:57227/1‑246 (MQ=255)                                                                                                                          cccGTTAACTTTAAAACCTCAAAATAAGCCCTGGAAAACGGCATTTCCATGGTGCACCAGGAATTGAATCTGGTGCGTCAGACCAGCGTAATGGATAACCTGTGGCTCGGACGTTATCCGCTGAAAGGACCGTTTGTGGATCACGCCAAAATCTATCGTGATACCAAAGCCATTTTTGACGAATTGGATATTGATATTGATCCGAAAGAAAAAGTGGCGAAAATTTCCGTTTCTCAAATGCAGATGATCGa                                                                                                   >  1:468746/1‑251 (MQ=255)                                                                                                                              ttAACTTTAAAACCTCAAAATAAGCCCTGGAAAACGGCATTTCCATGGTGCACCAGGAATTGAATCTGGTGCGTCAGACCAGCGTAATGGATAACCTGTGGCTCGGACGTTATCCGCTGAAAGGACCGTTTGTGGATCACGCCAAAATCTATCGTGATACCAAAGCCATTTTTGACGAGTTGGATATTGATATTGATCCGAAAGAAAAAGTGGCGAAACTTTCCGTTTCTCAAATGCAGATGATCGAAAt                                                                                                <  1:764932/250‑1 (MQ=255)                                                                                                                                  cTTTAAAACCTCAAAATAAGCCCTGGAAAACGGCATTTCCATGGTGCACCAGGAATTGAATCTGGTGCGTCAGACCAGCGTAATGGATAACCTGTGGCTCGGACGTTATCCGCTGAAAGGACCGTTTGTGGATCACGCCAAAATCTATCGTGATACCAAAGCCATTTTTGACGAGTTGgatat                                                                                                                                                               <  1:592339/183‑1 (MQ=255)                                                                                                                                  cTTTAAAACCTCAAAATAAGCCCTGGAAAACGGCATTTCCATGGTGCACCAGGAATTGAATCTGGTGCGTCAGACCAGCGTAATGGATAACCTGTGGCTCGGACGTTATCCGCTGAAAGGACCGTTTGTGGATCACGCCAAAATCTATCGTGATACCAAAGCCATTTTTGACGAGTTGGATATTGATATTGATCCGAAAGAAAAAGTGGCGAAACTTTCCGTTTCTCAAATGCAGATGATCGAAATTGCGa                                                                                           <  1:739975/251‑1 (MQ=255)                                                                                                                                          ccTCAAAATAAGCCCTGGAAAACGGCATTTCCATGGTGCACCAGGAATTGAATCTGGTGCGTCAGACCAGCGTAATGGATAACCTGTGGCTCGGACGTTATCCGCTGAAAGGACCGTTTGTGGATCACGCCAAAATCTATCGTGATACCAAAGCCATTTTTGACGAGTTGGATATTGATATTGATCCGAAAGAAAAAGTGGCGAAACTTTCCGTTTCTCAAATGCAGATGATCGAAATTGCGAAAGCGttt                                                                                   >  1:102336/1‑251 (MQ=255)                                                                                                                                           cTCAAAATAAGCCCTGGAAAACGGCATTTCCATGGTGCACCAGGAATTGAATCTGGTGCGTCAGACCAGCGTAATGGATAACCTGTGGCTCGGACGTTATCCGCTGAAAGGACCGTTTGTGGATCACGCCAAAATCTATCGTGATACCAAAGCCATTTTTGACGAGTTGgat                                                                                                                                                                 <  1:516032/172‑1 (MQ=255)                                                                                                                                               aaataagCCCTGGAAAACGGCATTTCCATGGTGCACCAGGAATTGAATCTGGTGCGTCAGACCAGCGTAATGGATAACCTGTGGCTCGGACGTTATCCGCTGAAAGGACCGTTTGTGGATCACGCCAAAATCTATCGTGATACCAAAGCCATTTTTGACGAGTTGGATATTGATATTGATCCGAAAGAAAAAGTGGCGAAACTTTCCGTTTCTCAAATGCAGATGATCGAAATTGCGAAAGCGTTTTCTta                                                                              >  1:446378/5‑251 (MQ=255)                                                                                                                                                     gCCCTGGAAAACGGCATTTCCATGGTGCACCAGGAATTGAATCTGGTGCGTCAGACCAGCGTAATGGATAACCTGTGGCTCGGACGTTATCCGCTGAAAGGACCGTTTGTGGATCACGCCAAAATCTATCGTGATACCAAAGCCATTTTTGACGAGTTGGATATTGATATTGATCCGAAAGAAAAAGTGGCGAAACTTTCCGTTTCTCAAATGCAGATGATCGa                                                                                                   <  1:67481/224‑1 (MQ=255)                                                                                                                                                     gCCCTGGAAAACGGCATTTCCATGGTGCACCAGGAATTGAATCTGGTGCGTCAGACCAGCGTAATGGATAACCTGTGGCTCGGACGTTATCCGCTGAAAGGACCGTTTGTGGATCACGCCAAAATCTATCGTGATACCAAAGCCATTTTTGACGAGTTGGATATTGATATTGATCCGAAAGAAAAAGTGGCGAAACTTTCCGTTTCTCAAATGCAGATGATCGAAATTGCGAAAGCGTTTTCTTATAACg                                                                         >  1:676797/1‑250 (MQ=255)                                                                                                                                                     gCCCTGGAAAACGGCATTTCCATGGTGCACCAGGAATTGAATCTGGTGCGTCAGACCAGCGTAATGGATAACCTGTGGCTCGGACGTTATCCGCTGAAAGGACCGTTTGTGGATCACGCCAAAATCTATAGTGATACCAAAGCCATTTTTGACGAGTTGGATATTGATATTGATCCGAAAGAAAAAGTGGCGAAACTTTCCGGTTCTCAAATGCAGATGATCGAAATTGCGAAAGCGTTTTCTTATAACg                                                                         >  1:802731/1‑250 (MQ=255)                                                                                                                                                                  gCATTTCCATGGTGCACCAGGAATTGAATCTGGTGCGTCAGACCAGCGTAATGGATAACCTGTGGCTCGGACGTTATCCGCTGAAAGGACCGTTTGTGGATCACGCCAAAATCTATCGTGATACCAAAGCCATTTTTGACGAGTTGgatat                                                                                                                                                               <  1:30161/151‑1 (MQ=255)                                                                                                                                                                  gCATTTCCATGGTGCACCAGGAATTGAATCTGGTGCGTCAGACCAGCGTAATGGATAACCTGTGGCTCGGACGTTATCCGCTGAAAGGACCGTTTGTGGATCACGCCAAAATCTATCGTGATACCAAAGCCATTTTTGACGAGTTGGATATTGATATTGATCCGAAAGAAAAAGTGGCGAAACTTTCCGTTTCTCAAATGCAGATGATCGAAATTGCGAAAGCGTTTTCTTATAACGCCAAAATCGTGAt                                                            <  1:232622/250‑1 (MQ=255)                                                                                                                                                                       tCCATGGTGCACCAGGAATTGAATCTGGTGCGTCAGACCAGCGTAATGGATAACCTGTGGCTCGGACGTTATCCGCTGAAAGGACCGTTTGTGGATCACGCCAAAATCTATCGTGATACCAAAGCCATTTTTGACGAGTTGGATATTGATATTGATCCGAAAGAAAAAGTGGCGAAACTTTCCGTTTCTCAAATGCAGATGATCGAAATTGCGAAAGCGTTTTCTTATAACGCCAAAATCGTGATTATGGa                                                      <  1:708713/251‑1 (MQ=255)                                                                                                                                                                       tCCATGGTGCACCAGGAATTGAATCTGGTGCGTCAGACCAGCGTAATGGATAACCTGTGGCTCGGACGTTATCCGCTGAAAGGACCGTTTGTGGATCACGCCAAAATCTATCGTGATACCAAAGCCATTTTTGACGAGTTGGATATTGATATTGATCCGAAAGAAAAAGTGGCGAAACTTTCCGTTTCTCAAATGCAGATGATCGAAATTGCGAAAGCGTTTTCTTATAACGCCAAAATCGTGATTATGGa                                                      <  1:464111/251‑1 (MQ=255)                                                                                                                                                                             gTGCACCAGGAATTGAATCTGGTGCGTCAGACCAGCGTAATGGATAACCTGTGGCTCGGACGTTATCCGCTGAAAGGACCGTTTGTGGATCACGCCAAAATCTATTGTGATACCAAAGCCATTTTTGACGAGTTGGATATTGATATTGATCCGAAAGAAAAAGTGGCGAAAc                                                                                                                               <  1:39035/172‑1 (MQ=255)                                                                                                                                                                              tGCACCAGGAATTGAATCTGGTGCGTCAGACCAGCGTAATGGATAACCTGTGGCTCGGACGTTATCCGCTGAAAGGACCGTTTGTGGATCACGCCAAAATCTATCGTGATACCAAAGCCATTTTTGACGAGTTGGATATTGATATTGATCCGAAAGAAAAAGTGGCGAAACTTTCCGTTTCTCAAATGCAGATGATCGAAATTGCGAAAGCGTTTTCTTATAACGCCAAAATCGTGATTATGGATGAAcc                                                <  1:273792/250‑1 (MQ=255)                                                                                                                                                                               gCACCAGGAATTGAATCTGGTGCGTCAGACCAGCGTAATGGATAACCTGTGGCTCGGACGTTATCCGCTGAAAGGACCGTTTGTGGATCACGCCAAAATCTATCGTGATACCAAAGCCATTTTTGACGAGTTGGATATTGATATTGATCCGAAAGAAAAAGTGGCGAAACTTTCCGTTTCTCAAATGCAGATGATCGAAATTGCGAAAGCGTTTTCt                                                                                >  1:84024/1‑217 (MQ=255)                                                                                                                                                                                  ccAGGAATTGAATCTGGTGCGTCAGACCAGCGTAATGGATAACCTGTGGCTCGGACGTTATCCGCTGAAAGGACCGTTTGTGGATCACGCCAAAATCTATCGTGATACCAAAGCCATTTTTGACGAGTTGGATATTGATATTGATCCGAAAGAAAAAGTGGCGAAACTTTCCGTTTCTCAAATGCAGATGATCGAAATTGCGAAAGCGTTTTCTTATAACGCCAAAATCGTGATTATGGATGAACCGGCAt                                           >  1:846545/1‑251 (MQ=255)                                                                                                                                                                                       aaTTGAATCTGGTGCGTCAGACCAGCGTAATGGATAACCTGTGGCTCGGACGTTATCCGCTGAAAGGACCGTTTGTGGATCACGCCAAAATCTATCGTGATACCAAAGCCATTTTTGACGAGTTGGATATTGATATTGATCCGAAAGAAAAAGTGGCGAAACTTTCCGTTTCTCAAATGCAGATGATCGAAATTGCGAAAGCGTTTTCTTATAACGCCAAAATCGTGATTATGGATGAACCGGCAtcctcc                                      <  1:184227/251‑1 (MQ=255)                                                                                                                                                                                            aaTCTGGTGCGTCAGACCAGCGTAATGGATAACCTGTGGCTCGGACGTTATCCGCTGAAAGGACCGTTTGTGGATCACGCCAAAATCTATCGTGATACCAAAGCCATTTTTGACGAGTTGGATATTGATATTGATCCGAAAGAAAAAGTGGCGAAACTTTCCGTTTCTCAAATGCAGATGATCGAAATTGCGAAAGCGTTTTCTTATAACGCCAAAATCGTGATTATGGATGAACCGGCATCCTCCCTTTc                                 <  1:197154/251‑1 (MQ=255)                                                                                                                                                                                                     cGTCAGACCAGCGTAATGGATAACCTGTGGCTCGGACGTTATCCGCTGAAAGGACCGTTTGTGGATCACGCCAAAATCTATCGTGATACCAAAGCCATTTTTGACGAGTTGGATATTGATATTGATCCGAAAGAAAAAGTGGCGAAACTTTCCGTTTCTCAAATGc                                                                                                             <  1:859679/166‑1 (MQ=255)                                                                                                                                                                                                              aGCGTAATGGATAACCTGTGGCTCGGACGTTATCCGCTGAAAGGACCGTTTGTGGATCACGCCAAAATCTATCGTGATACCAAAGCCATTTTTGACGAGTTGGATATTGATATTGATCCGAAAGAAAAAGTGGCGAAACTTTCCGTTTCTCAAATGCAGATGATCGAAATTGCGAAAGCGTTTTCTTATAACGCCAAAATCGTGATTATGGATGAACCGGCATCCTCCCTTTCCGAAAAAGAAGTGGaa                 >  1:204504/1‑249 (MQ=255)                                                                                                                                                                                                               gCGTAATGGATAACCTGTGGCTCGGACGTTATCCGCTGAAAGGACCGTTTGTGGATCACGCCAAAATCTATCGTGATACCAAAGCCATTTTTGACGAGTTGGATATTGATATTGATCCGAAAGAAAAAGTGGCGAAACTTTCCGTTTCTCAAATGCAGATGATCGAAATTGCGAAAGCGTTTTCTTATAACGCCAAAAt                                                                  >  1:4568/1‑199 (MQ=255)                                                                                                                                                                                                               gCGTAATGGATAACCTGTGGCTCGGACGTTATCCGCTGAAAGGACCGTTTGTGGATCACGCCAAAATCTATCGTGATACCAAAGCCATTTTTGACGAGTTGGATATTGATATTGATCCGAAAGAAAAAGTGGCGAAACTTTCCGTTTCTCAAATGCAGATGATCGAAATTGCGAAAGCGTTTTCTTATAACGCCAAAATCGTGATTATGGATGAACCGGCATCCTCCCTTTCCGAAAAAGAAGTGGAACAt              >  1:842464/1‑251 (MQ=255)                                                                                                                                                                                                               gCGTAATGGATAACCTGTGGCTCGGACGTTATCCGCTGAAAGGACCGTTTGTGGATCACGCCAAAATCTATCGTGATACCAAAGCCATTTTTGACGAGTTGGATATTGATATTGATCCGAAAGAAAAAGTGGCGAAACTTTCCGTTTCTCAAATGCAGATGATCGAAATTGCGAAAGCGTTTTCTTATAACGCCAAAATCGTGATTATGGATGAACCGGCATCCTCCCTTTCCGAAAAAGAAGTGGAACAt              >  1:469031/1‑251 (MQ=255)                                                                                                                                                                                                                cGTAATGGATAACCTGTGGCTCGGACGTTATCCGCTGAAAGGACCGTTTGTGGATCACGCCAAAATCTATCGTGATACCAAAGCCATTTTTGACGAGTTGGATATTGATATTGATCCGAAAGAAAAAGTGGCGAAACTTTCCGTTTCTCAAATGCAGATGATCGAAATTGCGAAAGCGTTTTCTTATAACGCCAAAATCGTGATTATGGATGAACCGGCATCCTCCCTTTCCGAAAAAGAAGTGGAACAt              <  1:586864/250‑1 (MQ=255)                                                                                                                                                                                                                cGTAATGGATAACCTGTGGCTCGGACGTTATCCGCTGAAAGGACCGTTTGTGGATCACGCCAAAATCTATCGTGATACCAAAGCCATTTTTGACGAGTTGGATATTGATATTGATCCGAAAGAAAAAGTGGCGAAACTTTCCGTTTCTCAAATGCAGATGATCGAAATTGCGAAAGCGTTTTCTTATAACGCCAAAATCGTGATTATGGATGAACCGGCATCCTCCCTTTCCGAAAAAGAAGTGGAACATc             <  1:640293/251‑1 (MQ=255)                                                                                                                                                                                                                    aTGGATAACCTGTGGCTCGGACGTTATCCGCTGAAAGGACCGTTTGTGGATCACGCCAAAATCTATCGTGATACCAAAGCCATTTTTGACGAGTTGGATATTGATATTGATCCGAAAGAAAAAGTGGCGAAACTTTCCGTTTCTCAAATGc                                                                                                             >  1:828499/1‑151 (MQ=255)                                                                                                                                                                                                                     tGGATAACCTGTGGCTCGGACGTTATCCGCTGAAAGGACCGTTTGTGGATCACGCCAAAATCTATCGTGATACCAAAGCCATTTTTGACGAGTTGGATATTGATATTGATCCGAAAGAAAAAGTGGCGAAACTTTCCGTTTCTCAAATGCAGATGATCGAAATTGCGAAAGCGTTTTCTTATAACGCCAAAATCGTGATTATGGATGAACCGGCATCCTCCCTTTCCGAAAAAGAAGTGGAACATCTGttt        >  1:665959/1‑251 (MQ=255)                                                                                                                                                                                                                      ggATAACCTGTGGCTCGGACGTTATCCGCTGAAAGGACCGTTTTTGGATCACGCCAAAATCTATCGTGATACCAAAGCCATTTTTGACGAGTTGGATATTGATATTGATCCGAAAGAAAAAGTGGCGAAACTTTCCGTTTCTCAAATGCAGATGATTGAAATTGCGAAAGCGTTTTCTTATAACGCCAAAATCGTGATTATGGATGAAACGGCATCCTCACTTTCCGAAAAAGAAGTGGAACATCTGTTTa       >  1:779549/1‑251 (MQ=255)                                                                                                                                                                                                                      ggATAACCTGTGGCTCGGACGTTATCCGCTGAAAGGACCGTTTGTGGATCACGCCAAAATCTATCGTGATACCAAAGCCATTTTTGACGAGTTGGATATTGATATTGATCCGAAAGAAAAAGTGGCGAAACTTTCCGTTTCTCAAATGCAGATGATCGAAATTGCGAAAGCGTTTTCTTATAACGCCAAAATCGTGATTATGGATGAACCGGCATCCTCCCTTTCCGAAAAAGAAGTGGAACATCTGTTTa       >  1:415124/1‑251 (MQ=255)                                                                                                                                                                                                                      ggATAACCTGTGGCTCGGACGTTATCCGCTGAAAGGACCGTTTGTGGATCACGCCAAAATCTATCGTGATACCAAAGCCATTTTTGACGAGTTGGATATTGATATTGATCCGAAAGAAAAAGTGGCGAAACTTTCCGTTTCTCAAATGCAGATGATCGAAATTGCGAAAGCGTTTTCTTATAACGCCAAAATCGTGATTATGGATGAACCGGCATCCTCCCTTTCCGAAAAAGAAGTGGAACATCTGTTTa       >  1:579223/1‑251 (MQ=255)                                                                                                                                                                                                                          aaCCTGTGGCTCGGACGTTATCCGCTGAAAGGACCGTTTGTGGATCACGCCAAAATCTATCGTGATACCAAAGCCATTTTTGACGAGTTGGATATTGATATTGATCCGAAAGAAAAAGTGGCGAAACTTTCCGTTTCTCAAATGCAGATGATCGAAATTGCGAAAGCGTTTTCTTATAACGCCAAAATCGTGATTATGGATGAACCGGCATCCTCCCTTTCCGAAAAAGAAGTGGAACATCTGTTTAAAAt   <  1:656632/251‑1 (MQ=255)                                                                                                                                                                                                                           aCCTGTGGCTCGGACGTTATCCGCTGAAAGGACCGTTTGTGGATCACGCCAAAATCTATCGTGATACCAAAGCCATTTTTGACGAGTTGGATATTGATATTGATCCGAAAGAAAAAGTGGCGAAACTTTCCGTTTCTCAAATGCAGATGATCGAAATTGCGAAAGCGTTTTCTTATAACGCCAAAATCGTGATTATGGATGAACCGGCATCCTCCCTTTCCGAAAAAGAAGTGGAACATCTGTTTAAAAt   >  1:29694/1‑250 (MQ=255)                                                                                                                                                                                                                           aCCTGTGGCTCGGACGTTATCCGCTGAAAGGACCGTTTGTGGATCACGCCAAAATCTATCGTGATACCAAAGCCATTTTTGACGAGTTGGATATTGATATTGATCCGAAAGAAAAAGTGGCGAAACTTTCCGTTTCTCAAATGCAGATGATCGAAATTGCGAAAGCGTTTTCTTATAACGCCAAAATCGTGATTATGGATGAACCGGCATCCTCCCTTTCCGAAAAAGAAGTGGAACATCTGTTTAAAAt   >  1:851045/1‑250 (MQ=255)                                                                                                                                                                                                                           aCCTGTGGCTCGGACGTTATCCGCTGAAAGGACCGTTTGTGGATCACGCCAAAATCTATCGTGATACCAAAGCCATTTTTGACGAGTTGGATATTGATATTGATCCGAAAGAAAAAGTGGCGAAACTTTCCGTTTCTCAAATGCAGATGATCGAAATTGCGAAAGCGTTTTCTTATAACGCCAAAATCGTGATTATGGATGAACCGGCATCCTCCCTTTCCGAAAAAGAAGTGGAACATCTGTTTAAAATc  <  1:818820/251‑1 (MQ=255)                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                    CGTCCGTTCACATTCTGTACACGCATTGATGGGGGAAAACGGTGCGGGTAAATCCACACTGCTAAAATGTTTATTTGGTATTTATGCTAAAGATGAAGGCGATATTTTATTTTTAGGCAAGCCCGTTAACTTTAAAACCTCAAAAGAAGCCCTGGAAAACGGCATTTCCATGGTGCACCAGGAATTGAATCTGGTGCGTCAGACCAGCGTAATGGATAACCTGTGACTCGGACGTTATCCGCTGAAAGGACCGTTTGTGGATCACGCCAAAATCTATCGTGATACCAAAGCCATTTTTGACGAGTTGGATATTGATATTGATCCGAAAGAAAAAGTGGCGAAACTTTCCGTTTCTCAAATGCAGATGATCGAAATTGCGAAAGCGTTTTCTTATAACGCCAAAATCGTGATTATGGATGAACCGACATCCTCCCTTTCCGAAAAAGAAGTGGAACATCTGTTTAAAATC  >  1/1395223‑1395691

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑