Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,379,100	A→C	intergenic (‑161/+231)	unknown ← / ← unknown	Apolipoprotein N‑acyltransferase / Copper homeostasis protein CutE/Magnesium and cobalt efflux protein CorC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,379,100	0	A	C	100.0%	138.1 / NA	51	intergenic (‑161/+231)	unknown/unknown	Apolipoprotein N‑acyltransferase / Copper homeostasis protein CutE/Magnesium and cobalt efflux protein CorC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (23/28);  total (23/28)

CCCCAATAATAAAAGGGTGCAAAGGCAAACACGCCGGCAGCGCCGAAAATGAGTGCGATAAGATAAGTGAAATATTTGTTCATAAGCGGGATAGCGCGCGTTTTCTCACGCAATTCCTAAATTTTCGTTATTTAATGAAATAAACGGCACGCGTCGGGAAACCACAGCTGTTCGAAACATTGTAACTACTTATATAAAAAGAAAAAAAACTTGGCTCCCCTCGTTCACAGGGGGAGCTGGGCGAAGCCCTGA‑‑G‑GGGG‑GGGAAAATCTCAATTTTTAAGCATGATCTTGTGAAAAAATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACCTTGCCCGTAAACGGAGGAAGGCAAGATATTTAAAAATATCAATGAATTATAATGTTTCGAACGGCTGTGGTCGGGAGACGCGCGCCACCGTTATCGTTCAACTCTCCCCCCGCCCCTCTTTTCTAAAGAGGGGAAAGAAGAAACTACGCCA  >  1/1378857‑1379338                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                   cccaaataatAAAAGGGTGCAAAGGAAAACACGCCGGCAGCGCCGAAAATGAGTGCGATAAGATAAGTGAAATATTTGTTCATAAGCGGGATAGCGCGCGTTTTCTCACGCAATTCCTAAATTTTCGTTATTTAATGAAATAAACGGCACGCGTCGGGAAACCACAGCTGTTCGAAACATTGTAACTACTTATATAAAAAGAAAAAAAACTTGGCTCCCCTCGTTCACAGGGGGAGCTGGGCGCAGccc                                                                                                                                                                                                                                               <  1:324663/245‑1 (MQ=255)    cAATAATAAAAGGGTGCAAAGGCAAACACGCCGGCAGCGCCGAAAATGAGTGCGATAAGATAAGTGAAATATTTGTTCATAAGCGGGATAGCGCGCGTTTTCTCACGCAATTCCTAAATTTTCGTTATTTAATGAAATAAACGGCACGCGTCGGGAAACCACAGCTGTTCGAAACATTGTAACTACTTATATAAAAAGAAAAAAAACTTGGCTCCCCTCGTTCACAGGGGGAGCTGGGCGCAGCCCTGA‑‑g‑g                                                                                                                                                                                                                                       <  1:770048/251‑1 (MQ=255)       taataaAAGGGTGCAAAGGCAAACACGCCGGCAGCGCCGAAAATGAGTGCGATAAGATAAGTGAAATATTTGTTCATAAGCGGGATAGCGCGCGTTTTCTCACGCAATTCCTAAATTTTCGTTATTTAATGAAATAAACGGCACGCGTCGGGAAACCACAGCTGTTCGAAACATTGTAACTACTTATATAAAAAGAAAAAAAACTTGGCTCCCCTCGTTCACAGGGGGAGCTGGGCGCAGCCCTGA‑‑g‑g                                                                                                                                                                                                                                       >  1:250302/1‑248 (MQ=255)       taataaAAGGGTGCAAAGGCAAACACGCCGGCAGCGCCGAAAATGAGTGCGATAAGATAAGTGAAATATTTGTTCATAAGCGGGATAGCGCGCGTTTTCTCACGCAATTCCTAAATTTTCGTTATTTAATGAAATAAACGGCACGCGTCGGGAAACCACAGCTGTTCGAAACATTGTAACTACTTATATAAAAAGAAAAAAAACTTGGCTCCCCTCGTTCACAGGGGGAGCTGGGCGCAGCCCTGA‑‑g‑g                                                                                                                                                                                                                                       >  1:667157/1‑248 (MQ=255)                               acGCCGGCAGCGCCGAAAATGAGTGCGATAAGATAAGTGAAATATTTGTTCATAAGCGGGATAGCGCGCGTTTTCTCACGCAATTCCTAAATTTTCGTTATTTAATGAAATAAACGGCACGCGTCGGGAAACCACAGCTGTTCGAAACATTGTAACTACTTATATAAAAAGAAAAAAAACTTGGCTCCCCTCGTTCACAGGGGGAGCTGGGCGCAGCCCTGAG‑G‑GGGG‑GGGAAAATCTCAATTTTTAAGCa                                                                                                                                                                                                            <  1:626301/251‑1 (MQ=255)                                                            aagataagTGAAATATTTGTTCATAAGCGGGATAGCGCGCGTTTTCTCACGCAATTCCTAAATTTTCGTTATTTAATGAAATAAACGGCACGCGTCGGGAAACCACAGCTGTTCGAAACATTGTAACTACTTATATAAAAAGAAAAAAAACTTGGCTCCCCTCGTTCACAGGGGGAGCTGGGCGCAGCCCTGAG‑G‑GGGG‑GGGAAAAtctc                                                                                                                                                                                                                        <  1:219079/210‑1 (MQ=255)                                                             agataagTGAAATATTTGTTCATAAGCGGGATAGCGCGCGTTTTCTCACGCAATTCCTAAATTTTCGTTATTTAATGAAATAAACGGCACGCGTCGGGAAACCACAGCTGTTCGAAACATTGTAACTACTTATATAAAAAGAAAAAAAACTTGGCTCCCCTCGTTCACAGGGGGAGCTGGGCGCAGCCCTGAG‑G‑GGGG‑GGGAAAATCTCAATTTTTAAGCATGATCTTGTGAAAAAATCAAGATAAATcc                                                                                                                                                                               >  1:242776/1‑250 (MQ=255)                                                             agataagTGAAATATTTGTTCATAAGCGGGATAGCGCGCGTTTTCTCACGCAATTCCTAAATTTTCGTTATTTAATGAAATAAACGGCACGCGTCGGGAAACCACAGCTGTTCGAAACATTGTAACTACTTATATAAAAAGAAAAAAAACTTGGCTCCCCTCGTTCACAGGGGGAGCTGGGCGCAGCCCTGA‑‑g‑g                                                                                                                                                                                                                                       <  1:392493/194‑1 (MQ=255)                                                                         tatTTGTTCATAAGCGGGATAGCGCGCGTTTTCTCACGCAATTCCTAAATTTTCGTTATTTAATGAAATAAACGGCACGCGTCGGGAAACCACAGCTGTTCGAAACATTGTAACTACTTATATAAAAAGAAAAAAAACTTGGCTCCCCTCGTTCACAGGGGGAGCTGGGCGCAGCCCTGAGGG‑GGGG‑GGGAAAATCTCAATTTTTAAGCATGATCTTGTGAAAAAATCAAGATAAATc                                                                                                                                                                                <  1:207560/238‑1 (MQ=255)                                                                                      gCGGGATAGCGCGCGTTTTCTCACGCAATTCCTAAATTTTCGTTATTTAATGAAATAAACGGCACGCGTCGGGAAACCACAGCTGTTCGAAACATTGTAACTACTTATATAAAAAGAAAAAAAACTTGGCTCCCCTCGTTCACAGGGGGAGCTGGGCGCAGCCCTGA‑‑g‑g                                                                                                                                                                                                                                       >  1:401754/1‑169 (MQ=255)                                                                                              gcgcgcGTTTTCTCACGCAATTCCTAAATTTTCGTTATTTAATGAAATAAACGGCACGCGTCGGGAAACCACAGCTGTTCGAAACATTGTAACTACTTATATAAAAAGAAAAAAAACTTGGCTCCCCTCGTTCACAGGGGGAGCTGGGCGCAGCCCTGAG‑G‑GGGG‑GGGAAAATCTCAATTTTTAAGCATGATCTTGTGAAAAAATCAAGAt                                                                                                                                                                                     <  1:20837/211‑1 (MQ=255)                                                                                                        tctcACGCAATTCCTAAATTTTCGTTATTTAATGAAATAAACGGCACGCGTCGGGAAACCACAGCTGTTCGAAACATTGTAACTACTTATATAAAAAGAAAAAAAACTTGGCTCCCCTCGTTCACAGGGGGAGCTGGGCGCAGCCCTGAG‑G‑GGGG‑GGGAAAATCTCAATTTTTAAGCATGATCTTGTGAAAAAATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACCTTGCCCGTAAACGGAGGAAGGc                                                                                                                                   <  1:3273/251‑1 (MQ=255)                                                                                                                 aTTCCTAAATTTTCGTTATTTAATGAAATAAACGGCACGCGTCGGGAAACCACAGCTGTTCGAAACATTGTAACTACTTATATAAAAAGAAAAAAAACTTGGCTCCCCTCGTTCACAGGGGGAGCTGGGCGCAGCCCTGAG‑G‑GGGG‑GGGAAAATCTCAATTTTTAAGCATGATCTTGTGAAAAAATCAAGAt                                                                                                                                                                                     <  1:459356/192‑1 (MQ=255)                                                                                                                 aTTCCTAAATTTTCGTTATTTAATGAAATAAACGGCACGCGTCGGGAAACCACAGCTGTTCGAAACATTGTAACTACTTATATAAAAAGAAAAAAAACTTGGCTCCCCTCGTTCACAGGGGGAGCTGGGCGCAGCCCTGAG‑G‑GGGG‑GGGAAAATCTCAATTTTTAAGCATGATCTTGTGAAAAAATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCAc                                                                                                                                                          >  1:500474/1‑219 (MQ=255)                                                                                                                                         gAAATAAACGGCACGCGTCGGGAAACCACAGCTGTTCGAAACATTGTAACTACTTATATAAAAAGAAAAAAAACTTGGCTCCCCTCGTTCACAGGGGGAGCTGGGCGCAGCCCTGA‑‑g‑gg                                                                                                                                                                                                                                      >  1:128512/1‑119 (MQ=255)                                                                                                                                                   gCACGCGTCGGGAAACCACAGCTGTTCGAAACATTGTAACTACTTATATAAAAAGAAAAAAAACTTGGCTCCCCTCGTTCACAGGGGGAGCTGGGCGCAGCCCTGAG‑G‑GGGG‑GGGAAAATCTCAATTTTTAAGc                                                                                                                                                                                                             <  1:791368/134‑1 (MQ=255)                                                                                                                                                          tctcccgaCCACAGCCGTTCGAAACATTGTAACTACTTATATAAAAAGAAAAAAAACTTGGCTCCCCTCGTTCACAGGGGGAGCTGGGCGCAGCCCTGAG‑G‑GGGG‑GGGAAAATCTCAATTTTTAAGCATGATCTTGTGAAAAAATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACCTTGCCCGTAAACGGAGGAAGGCAAGATATTTAAAAATATCAATGAATTATAATg                                                                                                   <  1:894417/226‑1 (MQ=255)                                                                                                                                                           ctcccgaCCACAGCCGTTCGAAACATTGTAACTACTTATATAAAAAGAAAAAAAACTTGGCTCCCCTCGTTCACAGGGGGAGCTGGGCGCAGCCCTGAG‑G‑GGGG‑GGGAAAATCTCAATTTTTAAGCATGATCTTGTGAAAAAATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACCTTGCCCGTAAACGGAGGAAGGCAAGATATTTAAAAat                                                                                                                    <  1:805056/209‑1 (MQ=255)                                                                                                                                                           ctcccgaCCACAGCCGTTCGAAACATTGTAACTACTTATATAAAAAGAAAAAAAACTTGGCTCCCCTCGTTCACAGGGGGAGCTGGGCGCAGCCCTGAG‑G‑GGGG‑GGGAAAATCTCAATTTTTAAGCATGATCTTGTGAAAAAATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACCTTGCCCGTAAACGGAGGAAGGCAAGATATTTAAAAATATCAATGAATTATAAt                                                                                                    >  1:149800/7‑231 (MQ=255)                                                                                                                                                              gAAACCACAGCTGTTCGAAACATTGTAACTACTTATATAAAAAGAAAAAAAACTTGGCTCCCCTCGTTCACAGGGGGAGCTGGGCGCAGCCCTGAG‑G‑GGGG‑GGGAAAATCTCAATTTTTAAGCATGATCTTGTGAAAAAATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACCTTGCCCGTAAACGGAGGAAGGCAAGATATTTAAAAATATCAATGAATTATAATGTTTCGAACGGCTGTGGTCggg                                                                              <  1:351018/250‑1 (MQ=255)                                                                                                                                                                aaCCACAGCTGTTCGAAACATTGTAACTACTTATATAAAAAGAAAAAAAACTTGGCTCCCCTCGTTCACAGGGGGAGCTGGGCGCAGCCCTGAG‑G‑GGGG‑GGGAAAATCTCAATTTTTAAGCATGATCTTGTGAAAAAATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACCTTGCCCGTAAACGGAGGAAGGCAAGATATTTAAAAATATCAATGAAtt                                                                                                         <  1:140671/221‑1 (MQ=255)                                                                                                                                                                aaCCACAGCTGTTCGAAACATTGTAACTACTTATATAAAAAGAAAAAAAACTTGGCTCCCCTCGTTCACAGGGGGAGCTGGGCGCAGCCCTGAG‑G‑GGGG‑GGGAAAATCTCAATTTTTAAGCATGATCTTGTGAAAAAATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACCTTGCCCGTAAACGGAGGAAGGCAAGATATTTAAAAATATCAATGAATtat                                                                                                       <  1:497779/223‑1 (MQ=255)                                                                                                                                                                aaCCACAGCTGTTCGAAACATTGTAACTACTTATATAAAAAGAAAAAAAACTTGGCTCCCCTCGTTCACAGGGGGAGCTGGGCGCAGCCCTGAG‑G‑GGGG‑GGGAAAATCTCAATTTTTAAGCATGATCTTGTGAAAAAATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACCTTGCCCGTAAACGGAGGAAGGCAAGATATTTAAAAATATCAATGAATtat                                                                                                       <  1:624899/223‑1 (MQ=255)                                                                                                                                                                aaCCACAGCTGTTCGAAACATTGTAACTACTTATATAAAAAGAAAAAAAACTTGGCTCCCCTCGTTCACAGGGGGAGCTGGGCGCAGCCCTGAG‑G‑GGGG‑GGGAAAATCTCAATTTTTAAGCATGATCTTGTGAAAAAATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACCTTGCCCGTAAACGGAGGAAGGCAAGATATTTAAAAATATCAATGAATTATAATGTTTCGAACGGCTGTGGTCGGgaga                                                                           >  1:876185/1‑251 (MQ=255)                                                                                                                                                                aaCCACAGCTGTTCGAAACATTGTAACTACTTATATAAAAAGAAAAAAAACTTGGCTCCCCTCGTTCACAGGGGGAGCTGGGCGCAGCCCTGAG‑G‑GGGG‑GGGAAAATCTCAATTTTTAAGCATGATCTTGTGAAAAAATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACCTTGCCCGTAAACGGAGGAAGGCAAGATATTTAAAAATATCAATGAATTATAATGTTTCGAACGGCTGTGGTCGGgaga                                                                           <  1:314580/251‑1 (MQ=255)                                                                                                                                                                aaCCACAGCTGTTCGAAACATTGTAACTACTTATATAAAAAGAAAAAAAACTTGGCTCCCCTCGTTCACAGGGGGAGCTGGGCGCAGCCCTGA‑‑g‑g                                                                                                                                                                                                                                       <  1:627409/95‑1 (MQ=255)                                                                                                                                                                        cTGTTCGAAACATTGTAACTACTTATATAAAAAGAAAAAAAACTTGGCTCCCCTCGTTCACAGGGGGAGCTGGGCGCAGCCCTGAG‑G‑GGGG‑GGGAAAATCTCAATTTTTAAGCATGATCTTGTGAAAAAATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACCTTGCCCGTAAACGGAGGAAGGCAAGATATTTAAAAATATCAATGAATTATAATGttt                                                                                                <  1:570037/222‑1 (MQ=255)                                                                                                                                                                          gTTCGAAACATTGTAACTACTTATATAAAAAGAAAAAAAACTTGGCTCCCCTCGTTCACAGGGGGAGCTGGGCGCAGCCCTGA‑‑G‑GGGGAGGGAAAATCTCAATTTTTAAGCATGATCTTGTGAAAAAATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACCTTGCCCGCAAACGGAGGAAGGCAAGATATTTAAAAATATCAATGAATTATAATg                                                                                                   >  1:40340/1‑217 (MQ=255)                                                                                                                                                                                  cATTGTAACTACTTATATAAAAAGAAAAAAAACTTGGCTCCCCTCGTTCACAGGGGGAGCTGGGCGCAGCCCTGAG‑G‑GGGG‑GGGAAAATCTCAATTTTTAAGCATGATCTTGTGAAAAAATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACCTTGCCCGTAAACGGAGGAAGGCAAGATATTTAAAAATATCAATGAATTATAATGTTTCGAACGGCTGTGGTCGGGAGACGCGCGCCACCGTTATCg                                                         >  1:41699/1‑251 (MQ=255)                                                                                                                                                                                  cATTGTAACTACTTATATAAAAAGAAAAAAAACTTGGCTCCCCTCGTTCACAGGGGGAGCTGGGCGCAGCCCTGAG‑G‑GGGG‑GGGAAAATCTCAATTTTTAAGCATGATCTTGTGAAAAAATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACCTTGCCCGTAAACGGAGGAAGGCAAGATATTTAAAAATATCAATGAATTATAATGTTTCGAACGGCTGTGGTCGGGAGACGCGCGCCACCGTTATCg                                                         >  1:81856/1‑251 (MQ=255)                                                                                                                                                                                  cATTGTAACTACTTATATAAAAAGAAAAAAAACTTGGCTCCCCTCGTTCACAGGGGGAGCTGGGCGCAGCCCTGA‑‑GCGGGG‑GGGAAAATCTCAATTTTTAAGCATGATCTTGTGAAAAAATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCAc                                                                                                                                                          >  1:233502/1‑154 (MQ=255)                                                                                                                                                                                    ttGTAACTACTTATATAAAAAGAAAAAAAACTTGGCTCCCCTCGTTCACAGGGGGAGCTGGGCGCAGCCCTGAG‑G‑GGGG‑GGGAAAATCTCAATTTTTAAGCATGATCTTGTGAAAAAATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACCTTGCCCGTAAACGGAGGAAGGc                                                                                                                                   <  1:170041/175‑1 (MQ=255)                                                                                                                                                                                    ttGTAACTACTTATATAAAAAGAAAAAAAACTTGGCTCCCCTCGTTCACAGGGGGAGCTGGGCGCAGCCCTGAG‑G‑GGGG‑GGGAAAATCTCAATTTTTAAGCATGATCTTGTGAAAAAATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACCTTGCCCGTAAACGGAGGAAGGCAAGATATTTAAAAATATCAATGAATTATAATGTTTCGAACGGCTGTGGTCGGGAGACGCGCGCCACCGTTATCGtt                                                       >  1:553490/1‑251 (MQ=255)                                                                                                                                                                                    ttGTAACTACTTATATAAAAAGAAAAAAAACTTGGCTCCCCTCGTTCACAGGGGGAGCTGGGCGCAGCCCTGA‑‑g‑ggg                                                                                                                                                                                                                                     >  1:48814/1‑77 (MQ=255)                                                                                                                                                                                      gTAACTACTTATATAAAAAGAAAAAAAACTTGGCTCCCCTCGTTCACAGGGGGAGCTGGGCGCAGCCCTGAG‑G‑GGGG‑GGGAAAATCTCAATTTTTAAGCATGATCTTGTGAAAAAATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACCTTGCCCGTAAACGGAGGAAGGCAAGATATTTAAAAATATCAATGAATTATAATGTTTCGAACGGCTGTGGTCGGGAGACGCGCGCCACCGTTATCGTTc                                                      >  1:52326/1‑250 (MQ=255)                                                                                                                                                                                      gTAACTACTTATATAAAAAGAAAAAAAACTTGGCTCCCCTCGTTCACAGGGGGAGCTGGGCGCAGCCCTGAG‑G‑GGGG‑GGGAAAATCTCAATTTTTAAGCATGATCTTGTGAAAAAATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACCTTGCCCGTAAACGGAGGAAGGCAAGATATTTAAAAATATCAATGAATTATAATGTTTCGAACAGCtg                                                                                      >  1:729028/1‑218 (MQ=255)                                                                                                                                                                                           tactTATATAAAAAGAAAAAAAACTTGGCTCCCCTCGTTCACAGGGGGAGCTGGGCGCAGCCCTGAG‑G‑GGGG‑GGGAAAATCTCAATTTTTAAGCATGATCTTGTGAAAAAATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACCTTGCCCGTAAACGGAGGAAGGCAAGATATTTAAAAATATCAATGAATTATaa                                                                                                     <  1:309034/198‑1 (MQ=255)                                                                                                                                                                                            actTATATAAAAAGAAAAAAAACTTGGCTCCCCTCGTTCACAGGGGGAGCTGGGCGCAGCCCTGAG‑G‑GGGG‑GGGAAAATCTCAATTTTTAAGCATGATCTTGTGAAAAAATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACCTTGCCCGTAAACGGAGGAAGGCAAGATATTTAAAAATATCAATGAATTATAATg                                                                                                   >  1:803778/1‑199 (MQ=255)                                                                                                                                                                                            actTATATAAAAAGAAAAAAAACTTGGCTCCCCTCGTTCACAGGGGGAGCTGGGCGCAGCCCTGAG‑G‑GGGG‑GGGAAAATCTCAATTTTTAAGCATGATCTTGTGAAAAAATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACCTTGCCCGTAAACGGAGGAAGGCAAGATATTTAAAAATATCAATGAATTATAATGTTTCGAACGGCTGTGGTCGGGAGACGCGCGCCACCGTTATCGTTCAActc                                                 >  1:63233/1‑249 (MQ=255)                                                                                                                                                                                             ctTATATAAAAAGAAAAAAAACTTGGCTCCCCTCGTTCACAGGGGGAGCTGGGCGCAGCCCTGAG‑G‑GGGG‑GGGAAAATCTCAATTTTTAAGCATGATCTTGTGAAAAAATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACCTTGCCCGTAAACGGAGGAAGGCAAGATATTTAAAAATATCAATGAATTATAATGTTTCGAACGGCTGTGGTCGGGAGACGCGCGCCACCGTTATCGTTCAActc                                                 <  1:657752/248‑1 (MQ=255)                                                                                                                                                                                                         gAAAAAAAACTTGGCTCCCCTCGTTCACAGGGGGAGCTGGGCGCAGCCCTGAG‑G‑GGGG‑GGGAAAATCTCAATTTTTAAGCATGATCTTGTGAAAAAATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACCTTGCCCGTAAACGGAGGAAGGCAAGATATTTaaaa                                                                                                                      <  1:387442/167‑1 (MQ=255)                                                                                                                                                                                                               aaaCTTGGCTCCCCTCGTTCACAGGGGGAGCTGGGCGCAGCCCTGAG‑G‑GGGG‑GGGAAAATCTCAATTTTTAAGCATGATCTTGTGAAAAAATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACCTTGCCCGTAAACGGAGGAAGGCAAGATATTTAAAAATATCAATGAATTATAATGTTTCGAACGGCTGTGGTCGGGAGACGCGCGCCACCGTTATCGTTCAACTCTCCCCCCGCCCCTCTTTTc                              <  1:730824/249‑1 (MQ=255)                                                                                                                                                                                                                  cTTGGCTCCCCTCGTTCACAGGGGGAGCTGGGCGCAGCCCTGAG‑G‑GGGG‑GGGAAAATCTCAATTTTTAAGCATGATCTTGTGAAAAAATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACCTTGCCCGTAAACGGAGGAAGGCAAGATATTTAAAAATATCAATGAATTATAATGTTTCGAACGGCTGTGGTCGGGAGACGCGCGCCACCGTTATCGTTCAACTCTCCCCCCGCCCCTCTTTTCTAAag                         <  1:288663/251‑1 (MQ=255)                                                                                                                                                                                                                  cTTGGCCCCCCTCGTTCACAGGGGGAGCTGGGCGCAGCCTTGAG‑G‑GGGG‑GGGAAAATCTCAATTTTTAAGCATGATCTTGTGAAAAAATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACCTTGCCCGTAAACGGAGGAAGGCAAGATATTTAAAAATATCAATGAATTATAATGTTTCGAACGGCTGTGGTCGGGAGACGCGCGCCACCGTTATCGTTCAACTCTCCCCCCGCCCCTCTTTTCTAAag                         <  1:754041/251‑1 (MQ=255)                                                                                                                                                                                                                     ggCTCCCCTCGTTCACAGGGGGAGCTGGGCGCAGCCCTGAG‑G‑GGGG‑GGGAAAATCTCAATTTTTAAGCATGATCTTGTGAAAAAATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACCTTGCCCGTAAACGGAGGAAGGCAAGATATTTAAAAATATCAATGAATTATaa                                                                                                     <  1:802416/172‑1 (MQ=255)                                                                                                                                                                                                                       cTCCCCTCGTTCACAGGGGGAGCTGGGCGCAGCCCTGAG‑G‑GGGG‑GGGAAAATCTCAATTTTTAAGCATGATCTTGTGAAAAAATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACCTTGCCCGTAAACGGAGGAAGGCAAGATATTTAAAAATATCAATGAATTATAATGTTTCGAACGGCTGTGGTCGGGAGACGCGCGCCACCGTTATCGTTCAACTCTCCCCCCGCCCCTCTTTTCTAAAGAgggg                    <  1:746834/251‑1 (MQ=255)                                                                                                                                                                                                                          cccTCGTTCACAGGGGGAGCTGGGCGCAGCCCTGAG‑G‑GGGG‑GGGAAAATCTCAATTTTTAAGCATGATCTTGTGAAAAAATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACCTTGCCCGTAAACGGAGGAAGGCAAGATATTTAAAAATATCAATGAATTATAATGTTTCGAACGGCTGTGGTCGGGAGACGCGCGCCACCGTTATCGTTCAACTCTCCCCCCGCCCCTCTTTTCTAAag                         >  1:516605/1‑243 (MQ=255)                                                                                                                                                                                                                             tCGTTCACAGGGGGAGCTGGGCGCAGCCCTGAG‑G‑GGGG‑GGGAAAATCTCAATTTTTAAGCATGATCTTGTGAAAAAATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACCTTGCCCGTAAACGGAGGAAGGCAAGATATTTAAAAATATCAATGAATTATAATGTTTCGAACGGCTGTGGTCGGGAGACGCGCGCCACCGTTATCGTTCAACTCTCCCCCCGCCCCTCTTTTCTAAAGAGGGGAaagaa              >  1:590203/1‑251 (MQ=255)                                                                                                                                                                                                                               gTTCACAGGGGGAGCTGGGCGCAGCCCTGAG‑G‑GGGG‑GGGAAAATCTCAATTTTTAAGCATGATCTTGTGAAAAAATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACCTTGCCCGTAAACGGAGGAAGGCAAGATATTTAAAAATATCAATGAATTATAATGTTTCGGACGGCTGTGGTCGGGAGACGCGCGCCACCGTTATCg                                                         >  1:425517/1‑206 (MQ=255)                                                                                                                                                                                                                                      gggggAGCTGGGCGCAGCCCTGAG‑G‑GGGG‑GGGAAAATCTCAATTTTTAAGCATGATCTTGTGAAAAAATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACCTTGCCCGTAAACGGAGGAAGGCAAGATATTTAAAAATATCAATGAATTATAATGTTTCGAACGGCTGTGGTCGGGAGACGCGCGCCACCGTTATCGTTCAACTCTCCCCCCGCCCCTCTTTTCTAAAGAGGGGAAAGAAGAAACTACg     >  1:882293/1‑251 (MQ=255)                                                                                                                                                                                                                                          gAGCTGGGCGCAGCCCTGAG‑G‑GGGG‑GGGAAAATCTCAATTTTTAAGCATGATCTTGTGAAAAAATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACCTTGCCCGTAAACGGAGGAAGGCAAGATATTTAAAAATATCAATGAATTATAATGTTTCGAACGGCTGTGGTCGGGAGACGCGCGCCACCGTTATCGTTCAACTCTCCCCCCGCCCCTCTTTTCTAAAGAGGGGAAAGAAGAAACTACGCca  >  1:480671/1‑250 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                   CCCCAATAATAAAAGGGTGCAAAGGCAAACACGCCGGCAGCGCCGAAAATGAGTGCGATAAGATAAGTGAAATATTTGTTCATAAGCGGGATAGCGCGCGTTTTCTCACGCAATTCCTAAATTTTCGTTATTTAATGAAATAAACGGCACGCGTCGGGAAACCACAGCTGTTCGAAACATTGTAACTACTTATATAAAAAGAAAAAAAACTTGGCTCCCCTCGTTCACAGGGGGAGCTGGGCGAAGCCCTGA‑‑G‑GGGG‑GGGAAAATCTCAATTTTTAAGCATGATCTTGTGAAAAAATCAAGATAAATCCCCCCTTCCGCCCTTCGGGCACCTTGCCCGTAAACGGAGGAAGGCAAGATATTTAAAAATATCAATGAATTATAATGTTTCGAACGGCTGTGGTCGGGAGACGCGCGCCACCGTTATCGTTCAACTCTCCCCCCGCCCCTCTTTTCTAAAGAGGGGAAAGAAGAAACTACGCCA  >  1/1378857‑1379338

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑