| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | 1 | 185,850 | G→A | H502H (CAC→CAT) | unknown ← | 2‑succinyl‑5‑enolpyruvyl‑6‑hydroxy‑3‑ cyclohexene‑1‑carboxylic‑acid synthase (EC 2.2.1.9) |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | 1 | 185,850 | 0 | G | A | 96.0% | 189.0 / NA | 77 | H502H (CAC→CAT) | unknown | 2‑succinyl‑5‑enolpyruvyl‑6‑hydroxy‑3‑ cyclohexene‑1‑carboxylic‑acid synthase (EC 2.2.1.9) |
| Reads supporting (aligned to +/- strand): ref base G (0/3); new base A (55/18); total (55/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.89e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CAAATTATCATGAAATAATTTGCTTTTGATGGTGTTATTTATTCGCCGACGACAGCATAGCTGATTTGTTCGATTAGGCGTTTATAAACGGTGCTGCCGTCATTCGGCGCCACTTTGATTTCGATGATGGTGGCTTCACGGCGTGAATAGGCTTGTTTCAGTACGGAACTCAAATCCGCCCA‑GGTGTAAGGGCGCGCATATTTT‑AAATCGAACATGGACGCCACTTGGG‑AAAATTCC‑GTGTGGTGCGGCATACGG‑TAGAATTTGTCTTTCACTTCCGCATCCACCGGCAACATATCGAAAATCGCCCCGCCGTTATTATTAATGATAAAAAGAATCGTCGGTTGGGTGATTTTTTTGAATAACGCCAGCGAGTTTAAATCATACAGCGCGGACGTGTCGCCCACTAACGCGACCACCGGCTGATTGGAACCGATACCGATGCCGGCGACGGTGGCAAGTAAACCGTCGATGCCACTGGCGCCGCG > 1/185611‑186095 | cAAATTATCATGAAATAATTTGCTTTTGATGGTGTTATTTATTCGCCGACGACAGCATAGCTGATTTGTTCGATTAGGCGTTTATAAACGGTGCTGCCGTCATTCGGCGCCACTTTGATTTCGATGATGGTGGCTTCAGGGCGTGAATAGGCTTGTTTCAGTACGGAACTCAAATCCGCCCA‑GGTGTAAGGGCGCGCATATTTT‑AAATCGAACATGGACGCCACTTGGG‑AAAATTCC‑GTATGGTGCGGCa < 1:48810/250‑1 (MQ=255)cAAATTATCATGAAATAATTTGCTTTTGATGGTGTTATTTATTCGCCGACGACAGCATAGCTGATTTGTTCGATTAGGCGTTTATAAACGGTGCTGCCGTCATTCGGCGCCACTTTGATTTCGATGATGGTGGCTTCACGGCGTGAATAGGCTTGTTTCAGTACGGAACTCAAATCCGCCCA‑GGTGTAAGGGCGCGCATATTTT‑AAATCGAACATGGACGCCACTTGGG‑AAAATTCC‑GTATGGTGCGGCAt < 1:39683/251‑1 (MQ=255)cAAATTATCATGAAATAATTTGCTTTTGATGGTGTTATTTATTCGCCGACGACAGCATAGCTGATTTGTTCGATTAGGCGTTTATAAACGGTGCTGCCGTCATTCGGCGCCACTTTGATTTCGATGATGGTGGCTTCACGGCGTGAATAGGCTTGTTTCAGTACGGAACTCAAATCCGCCCA‑GGTGTAAGGGCGCGCATATTTT‑AAATCGAACATGGACGCCACTTGGG‑AAAATTCC‑GTATGGTGCGGCAt < 1:241208/251‑1 (MQ=255)cAAATTATCATGAAATAATTTGCTTTTGATGGTGTTATTTATTCGCCGACGACAGCATAGCTGATTTGTTCGATTAGGCGTTTATAAACGGTGCTGCCGTCATTCGGCGCCACTTTGATTTCGATGATGGTGGCTTCACGGCGTGAATAGGCTTGTTTCAGTACGGAACTCAAATCCGCCCA‑GGTGTAAGGGCGCGCATATTTT‑AAATCGAACATGGACGCCACTTGGG‑AAAATTCC‑GTATGGTGCGGCAt < 1:789995/251‑1 (MQ=255) tATCATGAAATAATTTGCTTTTGATGGTGTTATTTATTCGCCGACGACAGCATAGCTGATTTGTTCGATTAGGCGTTTATAAACGGTGCTGCCGTCATTCGGCGCCACTTTGATTTCGATGATGGTGGCTTCACGGCGTGAATAGGCTTGTTTCAGTACGGAACTCAAATCCGCCCA‑GGTGTAAGGGCGCGCATATTTT‑AAATCGAACATGGACGCCACTTGGG‑AAAATTCC‑GTATGGTGCGGCATACGG‑t < 1:162865/251‑1 (MQ=255) tATCATGAAATAATTTGCTTTTGATGGTGTTATTTATTCGCCGACGACAGCATAGCTGATTTGTTCGATTAGGCGTTTATAAACGGTGCTGCCGTCATTCGGCGCCACTTTGATTTCGATGATGGTGGCTTCACGGCGTGAATAGGCTTGTTTCAGTACGGAACTCAAATCCGCCCA‑GGTGTAAGGGCGCGCATATTTT‑AAATCGAACATGGACGCCACTTGGG‑AAAATTCC‑GTATGGTGCGGCATACGG‑t < 1:614647/251‑1 (MQ=255) tATCATGAAATAATTTGCTTTTGATGGTGTTATTTATTCGCCGACGACAGCATAGCTGATTTGTTCGATTAGGCGTTTATAAACGGTGCTGCCGTCATTCGGCGCCACTTTGATTTCGATGATGGTGGCTTCACGGCGTGAATAGGCTTGTTTCAGTACGGAACTCAAATCCGCCCA‑GGTGTAAGGGCGCGCATATTTT‑AAATCGAACATGGACGCCACTTGGG‑AAAATTCC‑GTATGGTGCGGCATACGG‑t < 1:834055/251‑1 (MQ=255) aTCATGAAATAATTTGCTTTTGATGGTGTTATTTATTCGCCGACGACAGCATAGCTGATTTGTTCGATTAGGCGTTTATAAACGGTGCTGCCGTCATTCGGCGCCACTTTGATTTCGATGATGGTGGCTTCACGGCGTGAATAGGCTTGTTTCAGTACGGAACTCAAATCCGCCCA‑GGTGTAAGGGCGCGCATATTTT‑AAATCGAACATGGACGCCACTTGGG‑AAAATTCC‑GTATGGTGCGGCATACGG‑Ta > 1:45996/1‑251 (MQ=255) tGAAATAATTTGCTTTTGATGGTGTTATTTATTCGCCGACGACAGCATAGCTGATTTGTTCGATTAGGCGTTTATAAACGGTGCTGCCGTCATTCGGCGCCACTTTGATTTCGATGATGGTGGCTTCACGGCGTGAATAGGCTTGTTTCAGTACGGAACTCAAATCCGCCCA‑GGTGTAAGGGCGCGCATATTTT‑AAATCGAACATGGACGCCACTTGGG‑AAAATTCC‑GTATGGTGCGGCATACGG‑TAGAAt < 1:242862/251‑1 (MQ=255) tGAAATAATTTGCTTTTGATGGTGTTATTTATTCGCCGACGACAGCATAGCTGATTTGTTCGATTAGGCGTTTATAAACGGTGCTGCCGTCATTCGGCGCCACTTTGATTTCGATGATGGTGGCTTCACGGCGTGAATAGGCTTGTTTCAGTACGGAACTCAAATCCGCCCA‑GGTGTAAGGGCGCGCATATTTT‑AAATCGAACATGGACGCCACTTGGG‑AAAATTCC‑GTATGGTGCGGCATACGG‑TAGAAt < 1:777389/251‑1 (MQ=255) gAAATAATTTGCTTTTGATGGTGTTATTTATTCGCCGACGACAGCATAGCTGATTTGTTCGATTAGGCGTTTATAAACGGTGCTGCCGTCATTCGGCGCCACTTTGATTTCGATGATGGTGGCTTCACGGCGTGAATAGGCTTGTTTCAGTACGGAACTCAAATCCGCCCA‑GGTGTAAGGGCGCGCATATTTT‑AAATCGAACATGGACGCCACTTGGG‑AAAATTCC‑GTATGGTGCGGCAt > 1:692085/1‑240 (MQ=255) gCTTTTGATGGTGTTATTTATTCGCCGACGACAGCATAGCTGATTTGTTCGATTAGGCGTTTATAAACGGTGCTGCCGTCATTCGGCGCCACTTTGATTTCGATGATGGTGGCTTCACGGCGTGAATAGGCTTGTTTCAGTACGGAACTCAAATCCGCCCA‑GGTGTAAGGGCGCGCATATTTT‑AAATCGAACATGGACGCCACTTGGG‑AAAATTCC‑GTATGGTGCGGCATACGG‑TAGAATTTGTCTTTCa > 1:294764/1‑250 (MQ=255) gCTTTTGATGGTGTTATTTATTCGCCGACGACAGCATAGCTGATTTGTTCGATTAGGCGTTTATAAACGGTGCTGCCGTCATTCGGCGCCACTTTGATTTCGATGATGGTGGCTTCACGGCGTGAATAGGCTTGTTTCAGTACGGAACTCAAATCCGCCCA‑GGTGTAAGGGCGCGCATATTTT‑AAATCGAACATGGACGCCACTTGGG‑AAAATTCC‑GTATGGTGCGGCATACGG‑TAGAATTTGTCTTTCa > 1:175266/1‑250 (MQ=255) gtgtTATTTATTCGCCGACGACAGCATAGCTGATTTGTTCGATTAGGCGTTTATAAACGGTGCTGCCGTCATTCGGCGCCACTTTGATTTCGATGATGGTGGCTTCACGGCGTGAATAGGCTTGTTTCAGTACGGAACTCAAATCCGCCCA‑GGTGTAAGGGCGCGCATATTTT‑AAATCGAACATGGACGCCACTTGGG‑AAAATTCC‑GTATGGTGCGGCATACGG‑TAGAAttt > 1:420752/1‑232 (MQ=255) gtgtTATTTATTCGCCGACGACAGCATAGCTGATTTGTTCGATTAGGCGTTTATAAACGGTGCTGCCGTCATTCGGCGCCACTTTGATTTCGATGATGGTGGCTTCACGGCGTGAATAGGCTTGTTTCAGTACGGAACTCAAATCCGCCCA‑GGTGTAAGGGCGCGCATATTTT‑AAATCGAACATGGACGCCACTTGGG‑AAAATTCC‑GTATGGTGCGGCATACGG‑TAGAATTTGTCTTTCACTTCCGCATc > 1:699883/1‑250 (MQ=255) ttatttatTCGCCGACGACAGCATAGCTGATTTGTTCGATTAGGCGTTTATAAACGGTGCTGCCGTCATTCGGCGCCACTTTGATTTCGATGATGGTGGCTTCACGGCGTGAATAGGCTTGTTTCATTACGGAACTCAAATCCGCCCA‑GGTGTAAGGGCGCGCATATTTT‑AAATCGAACATGGACGCCACTTGGG‑AAAATTCC‑GTATGGTGCGGCATACGG‑TAGAATTTGTCTTTCACTTCCGCATCCAc < 1:543022/250‑1 (MQ=255) ccGACGACAGCATAGCTGATTTGTTCGATTAGGCGTTTATAAACGGTGCTGCCGTCATTCGGCGCCACTTTGATTTCGATGATGGTGGCTTCACGGCGTGAATAGGCTTGTTTCAGTACGGAACTCAAATCCGCCCA‑GGTGTAAGGGCGCGCATATTTT‑AAATCGAACATGGACGCCACTTGGG‑AAAATTCC‑GTATGGTGCGGCATACGG‑TAGAATTTGTCTTTCACTTCCGCATCCACCGGCAACatat < 1:887289/250‑1 (MQ=255) gacgacAGCATAGCTGATTTGTTCGATTAGGCGTTTATAAACGGTGCTGCCGTCATTCGGCGCCACTTTGATTTCGATGATGGTGGCTTCACGGCGTGAATAGGCTTGTTTCAGTACGGAACTCAAATCCGCCCA‑GGTGTAAGGGCGCGCATATTTT‑AAATCGAACATGGACGCCACTTGGG‑AAAATTCC‑GTATGGTGc < 1:288329/199‑1 (MQ=255) gacgacAGCATAGCTGATTTGTTCGATTAGGCGTTTATAAACGGTGCTGCCGTCATTCGGCGCCACTTTGATTTCGATGATGGTGGCTTCACGGCGTGAATAGGCTTGTTTCAGTACGGAACTCAAATCCGCCCA‑GGTGTAAGGGCGCGCATATTTT‑AAATCGAACATGGACGCCACTTGGG‑AAAATTCC‑GTATGGTGCGGCAt > 1:847581/1‑204 (MQ=255) cgacAGCATAGCTGATTTGTTCGATTAGGCGTTTATAAACGGTGCTGCCGTCATTCGGCGCCACTTTGATTTCGATGATGGTGGCTTCACGGCGTGAATAGGCTTGTTTCAGTACGGAACTCAAATCCGCCCA‑GGTGTAAGGGCGCGCATATTTT‑AAATCGAACATGGACGCCACTTGGG‑AAAATTCC‑GTATGGTGCGGCATACGG‑t < 1:539372/207‑1 (MQ=255) cgacAGCATAGCTGATTTGTTCGATTAGGCGTTTATAAACGGTGCTGCCGTCATTCGGCGCCACTTTGATTTCGATGATGGTGGCTTCACGGCGTGAATAGGCTTGTTTCAGTACGGAACTCAAATCCGCCCA‑GGTGTAAGGGCGCGCATATTTT‑AAATCGAACATGGACGCCACTTGGG‑AAAATTCC‑GTATGGTGCGGCATACGG‑TAGAATTTGTCTTTCACTTCCGCATCCACCGGCAACATATCGaaa < 1:688878/251‑1 (MQ=255) gCATAGCTGATTTGTTCGATTAGGCGTTTATAAACGGTGCTGCCGTCATTCGGCGCCACTTTGATTTCGATGATGGTGGCTTCACGGCGTGAATAGGCTTGTTTCAGTACGGAACTCAAATCCGCCCA‑GGTGTAAGGGCGCGCATATTTT‑AAATCGAACATGGACGCCACTTGGG‑AAAATTCC‑GTATGGTGCGGCATACGG‑TAGAATTTGTCTTTCACTTCCGCATCCACCGGCAACATATCGAAAATCg > 1:814910/1‑250 (MQ=255) tAGCTGATTTGTTCGATTAGGCGTTTATAAACGGTGCTGCCGTCATTCGGCGCCACTTTGATTTCGATGATGGTGGCTTCACGGCGTGAATAGGCTTGTTTCAGTACGGAACTCAAATCCGCCCA‑GGTGTAAGGGCGCGCATATTTT‑AAATCGAACATGGACGCCACTTGGG‑AAAATTCC‑GTATGGTGCGGCATACGG‑TAGAATTTGTCTTTCACTTCCGCATCCACCGGCAACATATCGAAAATCGccc > 1:191984/1‑250 (MQ=255) tGTTCGATTAGGCGTTTATAAACGGTGCTGCCGTCATTCGGCGCCACTTTGATTTCGATGATGGTGGCTTCACGGCGTGAATAGGCTTGTTTCAGTACGGAACTCAAATCCGCCCA‑GGTGTAAGGGCGCGCATATTTT‑AAATCGAACATGGACGCCACTTGGG‑AAAATTCC‑GTATGGTGCGGCATACGG‑TAGAATTTGTCTTTCACTTCCGCATCCACCGGCAACATATCGAAAATCGCCCCGCCGttatt > 1:158309/1‑251 (MQ=255) gTTCGATTAGGCGTTTATAAACGGTGCTGCCGTCATTCGGCGCCACTTTGATTTCGATGATGGTGGCTTCACGGCGTGAATAGGCTTGTTTCAGTACGGAACTCAAATCCGCCCA‑GGTGTAAGGGCGCGCATATTTT‑AAATCGAACATGGACGCCACTTGGG‑AAAATTCC‑GTATGGTGCGGCATACGG‑TAGAATTTGTCTTTCACTTCCGCATCCACCGGCAACATATCGAAAATCGCCCCGCCGttatta > 1:70851/1‑251 (MQ=255) aTTAGGCGTTTATAAACGGTGCTGCCGTCATTCGGCGCCACTTTGATTTCGATGATGGTGGCTTCACGGCGTGAATAGGCTTGTTTCAGTACGGAACTCAAATCCGCCCA‑GGTGTAAGGGCGCGCATATTTT‑AAATCGAACATGGACGCCACTTGGG‑AAAATTCC‑GTATGGTGCGGCATACGG‑TAGAATTTGTCTTTCACTTCCGCATCCACCGGCAACATATCGAAAATCGCCCCGCCGTTATTATTAAt > 1:896836/1‑251 (MQ=255) tttATAAACGGTGCTGCCGTCATTCGGCGCCACTTTGATTTCGATGATGGTGGCTTCACGGCGTGAATAGGCTTGTTTCAGTACGGAACTCAAATCCGCCCA‑GGTGTAAGGGCGCGCATATTTT‑AAATCGAACATGGACGCCACTTGGG‑AAAATTCC‑GTATGGTGCGGCATACGG‑t < 1:332210/176‑1 (MQ=255) tttATAAACGGTGCTGCCGTCATTCGGCGCCACTTTGATTTCGATGATGGTGGCTTCACGGCGTGAATAGGCTTGTTTCAGTACGGAACTCAAATCCGCCCA‑GGTGTAAGGGCGCGCATATTTT‑AAATCGAACATGGACGCCACTTGGG‑AAAATTCC‑GTATGGTGCGGCATACGG‑TAGAATTTGTCTTTCACTTCCGCATCCACCGGCAACATATCGAAAATCGCCccgccg > 1:617697/1‑232 (MQ=255) tttATAAACGGTGCTGCCGTCATTCGGCGCCACTTTGATTTCGATGATGGTGGCTTCACGGCGTGAATAGGCTTGTTTCAGTACGGAACTCAAATCCGCCCA‑GGTGTAAGGGCGCGCATATTTT‑AAATCGAACATGGACGCCACTTGGG‑AAAATTCC‑GTATGGTGCGGCATACGG‑TAGAATTTGTCTTTCACTTCCGCATCCACCGGCAACATATCGAAAATCGCCCCGCCGTTATTATTAATGATaaaaa > 1:208665/1‑251 (MQ=255) tttATAAACGGTGCTGCCGTCATTCGGCGCCACTTTGATTTCGATGATGGTGGCTTCACGGCGTGAATAGGCTTGTTTCAGTACGGAACTCAAATCCGCCCA‑GGTGTAAGGGCGCGCATATTTT‑AAATCGAACATGGACGCCACTTGGG‑AAAATTCC‑GTATGGTGCGGCATACGG‑TAGAATTTGTCTTTCACTTCCGCATCCACCGGCAACATATCGAAAATCGCCCCGCCGTTATTATTAATGATaaaa > 1:178448/1‑250 (MQ=255) gTGCTGCCGTCATTCGGCGCCACTTTGATTTCGATGATGGTGGCTTCACGGCGTGAATAGGCTTGTTTCAGTACGGAACTCAAATCCGCCCA‑GGTGTAAGGGCGCGCATATTTT‑AAATCGAACATGGACGCCACTTGGG‑AAAATTCC‑GTATGGTGCGGCAt > 1:187587/1‑161 (MQ=255) gTGCTGCCGTCATTCGGCGCCACTTTGATTTCGATGATGGTGGCTTCACGGCGTGAATAGGCTTGTTTCAGTACGGAACTCAAATCCGCCCA‑GGTGTAAGGGCGCGCATATTTT‑AAATCGAACATGGACGCCACTTGGG‑AAAATTCC‑GTATGGTGCGGCATACGG‑TAGAATTTGTCTTTCACTTCCGCATCCACCGGCAACATATCGAAAATCGCCCCGCCGttattattctgtctctttatacacatct > 1:381242/1‑230 (MQ=255) gTGCTGCCGTCATTCGGCGCCACTTTGATTTCGATGATGGTGGCTTCACGGCGTGAATAGGCTTGTTTCAGTACGGAACTCAAATCCGCCCA‑GGTGTAAGGGCGCGCATATTTT‑AAATCGAACATGGACGCCACTTGGG‑AAAATTCC‑GTATGGTGCGGCATACGG‑TAGAATTTGTCTTTCACTTCCGCATCCACCGGCAACATATCGAAAATCGCCCCGCCGTTATTATTAATGATAAAAAGAATCGTCgg > 1:352740/1‑251 (MQ=255) gTGCTGCCGTCATTCGGCGCCACTTTGATTTCGATGATGGTGGCTTCACGGCGTGAATAGGCTTGTTTCAGTACGGAACTCAAATCCGCCCA‑GGTGTAAGGGCGCGCATATTTT‑AAATCGAACATGGACGCCACTTGGG‑AAAATTCC‑GTATGGTGCGGCATACGG‑TAGAATTTGTCTTTCACTTCCGCATCCACCGGCAACATATCGAAAATCGCCCCGCCGTTATTATTAATGATAAAAAGAATCGTCgg > 1:91155/1‑251 (MQ=255) gTGCTGCCGTCATTCGGCGCCACTTTGATTTCGATGATGGTGGCTTCACGGCGTGAATAGGCTTGTTTCAGTACGGAACTCAAATCCGCCCA‑GGTGTAAGGGCGCGCATATTTT‑AAATCGAACATGGACGCCACTTGGG‑AAAATTCC‑GTATGGTGCGGCATACGG‑TAGAATTTGTCTTTCACTTCCGCATCCACCGGCAACATATCGAAAATCGCCCCGCCGTTATTATTAATGATAAAAAGAATCGTCgg > 1:654628/1‑251 (MQ=255) gTGCTGCCGTCATTCGGCGCCACTTTGATTTCGATGATGGTGGCTTCACGGCGTGAATAGGCTTGTTTCAGTACGGAACTCAAATCCGCCCA‑GGTGTAAGAGCGCGCATATTTT‑AAATCGAACATGGACGCCACTTGGG‑AAAATTCC‑GTATGGTGCGGCATACGG‑TAGAATTTGTCTTTCACTTCCGCATCCACCGGCAACATATCGAAAATCGCCCCGCCGTTATTATTAATGATAAAAAGAATCGTCgg > 1:890889/1‑251 (MQ=255) gctgcCGTCATTCGGCGCCACTTTGATTTCGATGATGGTGGCTTCACGGCGTGAATAGGCTTGTTTCAGTACGGAACTCAAATCCGCCCA‑GGTGTAAGGGCGCGCATATTTT‑AAATCGAACATGGACGCCACTTGGG‑AAAATTCC‑GTATGGTGCGGCATACGG‑TAGAATTTGTCTTTCACTTCCGCATCCACCGGCAACATATCGAAAATCGCCCCGCCGTTATTATTAATGATAAAAAGAATCGTCGGtt > 1:78498/1‑251 (MQ=255) cTTTGATTTCGATGATGGTGGCTTCACGGCGTGAATAGGCTTGTTTCAGTACGGAACTCAAATCCGCCCA‑GGTGTAAGGGCGCGCATATTTT‑AAATCGAACATGGACGCCACTTGGG‑AAAATTCC‑GTATGGTGCGGCATACGG‑TAGAATTTGTCTTTCACTTCCGCATCCACCGGCAACATATCGAAAATCGCCCCGCCGTTATTATTAATGATAAAAAGAATCGTCGGTTGGGTGATTTTTTTGAATaa > 1:801195/1‑250 (MQ=255) gATTTCGATGATGGTGGCTTCACGGCGTGAATAGGCTTGTTTCAGTACGGAACTCAAATCCGCCCA‑GGTGTAAGGGCGCGCATATTTT‑AAATCGAACATGGACGCCACTTGGG‑AAAATTCC‑GTATGGTGCGGCATACGG‑TAGAATTTGTCTTTCACTTCCGCATCCACCGGCAACATATCGAAAATCGCCCCGCCGTTATTATTaa > 1:576130/1‑206 (MQ=255) atgGTGGCTTCACGGCGTGAATAGGCTTGTTTCAGTACGGAACTCAAATCCGCCCA‑GGTGTAAGGGCGCGCATATTTT‑AAATCGAACATGGACGCCACTTGGG‑AAAATTCC‑GTATGGTGCGGCATACGG‑TAGAATTTGTCTTTCACTTCCGCATCCACCGGCAACATATCGAAAATCGCCCCGCCGTTATTATTAATGATAAAAAGAATCGTCGGTTGGGTGATTTTTTTGAATAACGCCAGCGAGTTTaa > 1:808029/1‑251 (MQ=255) gtggCTTCACGGCGTGAATAGGCTTGTTTCAGTACGGAACTCAAATCCGCCCA‑GGTGTAAGGGCGCGCATATTTT‑AAATCGAACATGGACGCCACTTGGG‑AAAATTCC‑GTATGGTGCGGCATACGG‑TAGAATTTGTCTTTCACTTCCGCATCCACCGGCAACATATCGAAAATCGCCCCGCCGTTATTATTAATGATAAAAAGAATCGTCGGTTGGGTGATTTTTTTGAAt > 1:824258/1‑231 (MQ=255) ctccggggggtaaatgggtttttttCAGTCCGAAACACAAA‑CCCCCCAGGGTGTAGGGGCCCGCATTTTTA‑AAACCAAACAGGGCCCCCACTTGGA‑AAAATCCC‑GTATGGGGCGGCATGCGG‑TAGATTTTTTTTTCCCCTCCCCCATCCCCCGGCAACATAACAAAAACCCCCCCGCCGTTATTATTAATGATAAAAAGAATCGTCGGTTGGGTGATTTTTTTGAATAACGCCAGCGAGTTTAAATCATAc < 1:346646/229‑1 (MQ=255) gCGTGAATAGGCTTGTTTCAGTACGGAACTCAAATCCGCCCA‑GGTGTAAGGGCGCGCATATTTT‑AAATCGAACATGGACGCCACTTGGG‑AAAATTCC‑GTATGGTGCGGCATACGG‑TAGAATTTGTCTTTCACTTCCGCATCCACCGGCAACATATCGAAAATCGCCCCGCCGTTATTATTAATGATAAAAAGAATCGTCGGTTGGGTGATTTTTTTGAATAACGCCAGCGAGTTTAAATCATACAGCGCgg > 1:418596/1‑251 (MQ=255) aaaagaggtttGTTTCAGTACGAACCAAAAACCCCCCCA‑GGTGAAGGGGCGCGCATTTTTA‑AAATCAAACAGGGCCGCCATTTGGA‑AAAATTCC‑TTAGGGTGGGGCATGGGG‑AAAAGTTTTTTTTTCCCTCCCCCACCCCCCGACAACATAGCAAAAACCGCCCCGCCGTTATTATTAATGATAAAAAGAATCGTCGGTTGGGTGATTTTTTTGAATAACGCCAGCGAGTTTAAATCATACAGCGCGGACg < 1:183630/242‑1 (MQ=255) gAATAGGCTTGTTTCAGTACGGAACTCAAATCCGCCCA‑GGTGTAAGGGCGCGCATATTTT‑AAATCGAACATGGACGCCACTTGGG‑AAAATTCC‑GTATGGTGCGGCATACGG‑TAGAATTTGTCTTTCACTTCCGCATCCACCGGCAACATATCGAAAATCGCCCCGCCGttatta > 1:879493/1‑174 (MQ=255) aaatagttttttttCAGTGCGGACCTCAAACCCCCCCG‑GGTGAAGGGGCGCG‑ATTTTTTAAAATAGAACTGGGCCCCCACTTGGA‑AAAATTCC‑GTAGGGTGCGGCAT‑CGGTTAGATTTTTTTTTCCCCTTCCCCACCCCCCGGAAAAATATCAAAAACCCCCCCGCCGTTATTATTAATGATAAAAAGAATCGTCGGTTGGGTGATTTTTTTGAATAACGCCAGCGAGTTTAAATCATACAGCGCGGACg < 1:476264/239‑1 (MQ=255) ggCTTGTTTCAGTACGGAACTCAAATCCGCCCA‑GGTGTAAGGGCGCGCATATTTT‑AAATCGAACATGGACGCCACTTGGG‑AAAATTCC‑GTATGGTGCGGCATACGG‑TAGAATTTGTCTTTCACTTCCGCATCCACCGGCAACATATCGAAAATCGCCCCGCCGTTATTATTAATGATAAAAAGAATCGTCGGTTGGGTGATTTTTTTGAATAACGCCAGCGAGTTTAAATCATACAGCGCGGACGTGTCg > 1:299464/1‑250 (MQ=255) gTTTCAGTACGGAACTCAAATCCGCCCA‑GGTGTAAGGGCGCGCATATTTT‑AAATCGAACATGGACGCCACTTGGG‑AAAATTCC‑GTATGGTGCGGCATACGG‑TAGAATTTGTCTTTCACTTCCGCATCCACCGGCAACATATCGAAAATCGCCCCGCCGTTATTATTAATGATAAAAAGAATCGTCGGTTGGGTGATTTTTTTGAATAACGCCAGCGAGTTTAAATCATACAGCGCGGACGTGTCGCCCa > 1:186798/1‑249 (MQ=255) tttCAGTACGGAACTCAAATCCGCCCA‑GGTGTAAGGGCGCGCATATTTT‑AAATCGAACATGGACGCCACTTGGG‑AAAATTCC‑GTATGGTGCGGCATACGG‑TAGAATTTGTCTTTCACTTCCGCATCCACCGGCAACATATCGAAAATCGCCCCGCCGTTATTATTAATGATAAAAAGAATCGTCGGTTGGGTGATTTTTTTGAATAACGCCAGCGAGTTTAAATCATACAGCGCGGACGTGTCGCCCACTa > 1:142745/1‑251 (MQ=255) gTACGGAACTCAAATCCGCCCA‑GGTGTAAGGGCGCGCATATTTT‑AAATCGAACATGGACGCCACTTGGG‑AAAATTCC‑GTATGGTGCGGCATACGG‑TAGAATTTGTCTTTCACTTCCGCATCCACCGGCAACATATCGAAAATCGCCCCGCCGTTATTATTAATGATAAAAAGAATCGTCGGTTGGGTGATTTTTTTGAATAACGCCAGCGAGTTTAAATCATACAGCGCGGACGTGTCGCCCACTaa > 1:778286/1‑247 (MQ=255) ctgggagttgtgtagaagagaagggTGTAGGGGCGCGAATTTTTA‑AAATCGAACAGGGCCGCCA‑TTGGG‑AAAATTCCGGTTGGGGGCGGCAGGGGG‑TAGATTTTGTTTTTCACTCCCGCACCCCCCGAAAAAATATAAAAAATCGCCCCGCCGTTATTATTAATGATAAAAAGAATCGTCGGTTGGGTGATTTTTTTGAATAACGCCAGCGAGTTTAAATCATACAGCGCGGACGTGTCGCCCACTAAcg < 1:681912/227‑1 (MQ=255) cgaacccaaaccccGCCAA‑GGTGAAGGGGCGCACATATTTA‑AAACCAAACATGGCCCCCACTGGGA‑AAAATTCC‑GTGGGGGGCGGCATACGG‑TAAATTTTTTCTTTCCCTCCCCCACCCCCCGCAAAAAAAACAAAAACCCCCCCGCCGTTATTATTAATGATAAAAAGAATCGTCGGTTGGGTGATTTTTTTGAATAACGCCAGCGAGTTTAAATCATACAGCGCGGACGTGTCGCCCACTAACGCGacc < 1:541746/239‑1 (MQ=255) ccAAACCCGCCGA‑GGTGGAGGGGCGCGATTATTTA‑AACTCGAACGGGGACGCCA‑TTGGGAAAAATTCC‑TTGGGGTGGGGCATACGG‑AAGTTTTTTTCTTTCCCTCCCGCACCCACCGGCAACATAAAAAAATTCGCCCCGCCGTTATTATTAATGATAAAAAGAATCGTCGGTTGGGTGATTTTTTTGAATAACGCCAGCGAGTTTAAATCATACAGCGCGGACGTGTCGCCCACTAACGCGACCACCGGc < 1:604443/250‑1 (MQ=255) cggggTGTAGGGGCGCGAATTTTTA‑AAATCGAACATGGCCGCCTTTTGGA‑AAAATTCC‑GTGTGGTGCGGCATACGG‑TAAATTTTTTTTTTCCCTCCCCCACCCCCCGAAAACATATCAAAAACCGCCCCGCCGTTATTATTAATGATAAAAAGAATCGTCGGTTGGGTGATTTTTTTGAATAACGCCAGCGAGTTTAAATCATACAGCGCGGACGTGTCGCCCACTAACGCGACCACCGGCTGATTGGAac < 1:20360/248‑1 (MQ=255) ggTGTAAGGGCGCGCATATTTT‑AAATCGAACATGGACGCCACTTGGG‑AAAATTCC‑GTATGGTGCGGCATACGG‑TAGAATTTGTCTTTCACTTCCGCATCCACCGGCAACATATCGAAAATCGCCCCGCCGTTATTATTAATGATAAAAAGAATCGTCGGTTGGGTGATTTTTTTGAATAACGCCAGCGAGTTTAAATCATACAg > 1:355599/1‑204 (MQ=255) ggTGTAAGGGCGCGCATATTTT‑AAATCGAACATGGACGCCACTTGGG‑AAAATTCC‑GTATGGTGCGGCATACGG‑TAGAATTTGTCTTTCACTTCCGCATCCACCGGCAACATATCGAAAATCGCCCCGCCGTTATTATTAATGATAAAAAGAATCGTCGGTTGGGTGATTTTTTTGAATAACGCCAGCGAGTTTAAATCATACAGCGCGGACGTGTCGCCCAc > 1:186272/1‑222 (MQ=255) ggTGTAAGGGCGCGCATATTTT‑AAATCGAACATGGACGCCACTTGGG‑AAAATTCC‑GTATGGTGCGGCATACGG‑TAGAATTTGTCTTTCACTTCCGCATCCACCGGCAACATATCGAAAATCGCCCCGCCGTTATTATTAATGATAAAAAGAATCGTCGGTTGGGTGATTTTTTTGAATAACGCCAGCGAGTTTAAATCATACAGCGCGGACGTGTCGCCCACTAACGCGACCACCGGCTGATTGGAaccga > 1:384326/1‑251 (MQ=255) ggTGTAAGGGCGCGCATATTTT‑AAATCGAACATGGACGCCACTTGGG‑AAAATTCC‑GTATGGTGCGGCATACGG‑TAGAATTTGTCTTTCACTTCCGCATCCACCGGCAACATATCGAAAATCGCCCCGCCGTTATTATTAATGATAAAAAGAATCGTCGGTTGGGTGATTTTTTTGAATAACGCCAGCGAGTTTAAATCATACAGCGCGGACGTGTCGCCCACTAACGCGACCACCGGCTGATTGGAaccga > 1:163176/1‑251 (MQ=255) ggTGTAAGGGCGCGCATATTTT‑AAATCGAACATGGACGCCACTTGGG‑AAAATTCC‑GTATGGTGCGGCATACGG‑TAGAATTTGTCTTTCACTTCCGCATCCACCGGCAACATATCGAAAATCGCCCCGCCGTTATTATTAATGATAAAAAGAATCGTCGGTTGGGTGATTTTTTTGAATAACGCCAGCGAGTTTAAATCATACAGCGCGGACGTGTCGCCCACTAACGCGACCACCGGCTGATTGGAaccga > 1:304088/1‑251 (MQ=255) ggTGTAAGGGCGCGCATATTTT‑AAATCGAACATGGACGCCACTTGGG‑AAAATTCC‑GTATGGTGCGGCATACGG‑TAGAATTTGTCTTTCACTTCCGCATCCACCGGCAACATATCGAAAATCGCCCCGCCGTTATTATTAATGATAAAAAGAATCGTCGGTTGGGTGATTTTTTTGAATAACGCCAGCGAGTTTAAATCATACAGCGCGGACGTGTCGCCCACTAACGCGACCACCGGCTGATTGGAaccga > 1:499438/1‑251 (MQ=255) ggTGTAAGGGCGCGCATATTTT‑AAATCGAACATGGACGCCACTTGGG‑AAAATTCC‑GTATGGTGCGGCATACGG‑TAGAATTTGTCTTTCACTTCCGCATCCACCGGCAACATATCGAAAATCGCCCCGCCGTTATTATTAATGATAAAAAGAATCGTCGGTTGGGTGATTTTTTTGAATAACGCCAGCGAGTTTAAATCATACAGCGCGGACGTGTCGCCCACTAACGCGACCACCGGCTGATTGGAaccga > 1:686085/1‑251 (MQ=255) ggTGTAAGCGCGCGCATATTTT‑AAATCGAACATGGACGCCACTTGGG‑AAAATTCC‑TTATGGTGCGGCACACGG‑TAGAATTTGTCTTTCACTTCCGCATCCACCGGCAACATATCGAAAATCGCCCCGCCGTTATTATTAATGATAAAAAGTATCGTCGGATGGGTGATTTTTTTGAAt > 1:795139/1‑178 (MQ=255) gtgtAAGGGCGCGCATATTTT‑AAATCGAACATGGACGCCACTTGGG‑AAAATTCC‑GTATGGTGCGGCATACGG‑t > 1:43457/1‑73 (MQ=255) gtgtAAGGGCGCGCATATTTT‑AAATCGAACATGGACGCCACTTGGG‑AAAATTCC‑GTATGGTGCGGCATACGG‑TAGAATTTGTCTTTCACTTCCGCATCCACCGGCAACATATCGAAAATCGCCCCGCCGttattatta > 1:28121/1‑138 (MQ=255) gtgtAAGGGCGCGCATATTTT‑AAATCGAACATGGACGCCACTTGGG‑AAAATTCC‑GTATGGTGCGGCATACGG‑TAGAATTTGTCTTTCACTTCCGCATCCACCGGCAACATATCGAAAATCGCCCCGCCGTTATTATTAATGATAAAAAGAAtcgtc > 1:827166/1‑156 (MQ=255) gtgtAAGGGCGCGCATATTTT‑AAATCGAACATGGACGCCACTTGGG‑AAAATTCC‑GTATGGTGCGGCATACGG‑TAGAATTTGTCTTTCACTTCCGCATCCACCGGCAACATATCGAAAATCGCCCCGCCGTTATTATTAATGATAAAAAGAATCGTCGGTTGGGTGATTTTTTTGAATAACGCCAGCGAGTTTAAATCATACAGCGCGGACGTGTCGCCCACTAACGCGACCACCGGCTGATTGGAaccgat > 1:750835/1‑251 (MQ=255) gtgtAAGGGCGCGCATATTTT‑AAATCGAACATGGACGCCACTTGGG‑AAAATTCC‑GTATGGTGCGGCATACGG‑TAGAATTTGTCTTTCACTTCCGCATCCACCGGCAACATATCGAAAATCGCCCCGCCGTTATTATTAATGATAAAAAGAATCGTCGGTTGGGTGATTTTTTTGAATAACGCCAGCGAGTTTAAATCATACAGCGCGGACGTGTCGCCCACTAACGCGACCACCGGCTGATTGGAaccgat > 1:445592/1‑251 (MQ=255) gtgtAAGGGCGCGCATATTTT‑AAATCGAACATGGACGCCACTTGGG‑AAAATTCC‑GTATGGTGCGGCATACGG‑TAGAATTTGTCTTTCACTTCCGCATCCACCGGCAACATATCGAAAATCGCCCCGCCGTTATTATTAATGATAAAAAGAATCGTCGGTTGGGTGATTTTTTTGAATAACGCCAGCGAGTTTAAATCATACAGCGCGGACGTGTCGCCCACTAACGCGACCACCGGCTGATTGGAaccgat > 1:618019/1‑251 (MQ=255) gtgtAAGGGCGCGCATATTTT‑AAATCGAACATGGACGCCACTTGGG‑AAAATTCC‑GTATGGTGCGGCATACGG‑TAGAATTTGTCTTTCACTTCCGCATCCACCGGCAACATATCGAAAATCGCCCCGCCGTTATTATTAATGATAAAAAGAATCGTCGGTTGGGTGATTTTTTTGAATAACGCCAGCGAGTTTAAATCATACAGCGCGGACGTGTCGCCCACTAACGCGACCACCGGCTGATTGGAaccgat > 1:190042/1‑251 (MQ=255) tatTTT‑AAATCGAACATGGACGCCACTTGGG‑AAAATTCC‑GTATGGTGCGGCATACGG‑TAGAATTTGTCTTTCACCTCCGCATCCACCGGCAACATATCGAAAATCGCCCCGCCGTTATTATTAATGATAAAAAGAATCGTCGGTTGGGTGATTTTTTTGAATAACGCCAGCGAGTTTAAATCATACAGCGCGGACGTGTCGCCCACTAACGCGACCACCGGCTGATTGGAACCGATACCGATGCCGGCGa > 1:896409/1‑250 (MQ=255) gAACATGGACGCCACTTGGG‑AAAATTCC‑GTATGGTGCGGCATACGG‑TAGAATTTGTCTTTCACTTCCGCATCCACCGGCAACATATCGAAAAGCGCCCCGCCGTTATTATTAATGATAAAAAGAATTGTCGGTTGGGTGATTTTTTTGAATAACGCCAGCGAGTTTAAATCATACAGCGCGGACGTGTCGTCCACTAACGCGACCACCGGCTGATTGGAac > 1:399568/1‑221 (MQ=255) aaCATGGACGCCACTTGGG‑AAAATTCC‑GTATGGTGCGGCATACGG‑TAGAATTTGTCTTTCACTTCCGCATCCACCGGCAACATATCGAAAATCGCCCCGCCGTTATTATTAATGATAAAAAGAATCGTCGGTTGGGTGATTTTTTTGAATAACGCCAGCg > 1:425807/1‑160 (MQ=255) ggACGCCACTTGGG‑AAAATTCC‑GTATGGTGCGGCATACGG‑TAGAATTTGTCTTTCACTTCCGCATCCACCGGCAACATATCGAAAATCGCCCCGCCGTTATTATTAATGATAAAAAGAATCGTCGGTTGGGTGATTTTTTTGAATAACGCCAGCGAGTTTAAATCATACAg > 1:879236/1‑171 (MQ=255) aCTTGGG‑AAAATTCC‑GTATGGTGCGGCATACGG‑TAGAATTTGTCTTTCACTTCCGCATCCACCGGCAACATATCGAAAATCGCCCCGCCGTTATTATTAATGATAAAAAGAATCGTCGGTTGGGTGATTTTTTTGAATAACGCCAGCGAGTTTAAATCATACAGCGCGGACGTGTCGCCCACTAACGCGACCACCGGCTGATTGGAACCGATACCGATGCCGGCGACGGTGGCAAGTAAACCGTCGATGc > 1:315409/1‑250 (MQ=255) gg‑AAAATTCC‑GTATGGTGCGGCATACGG‑TAGAATTTGTCTTTCACTTCCGCATCCACCGGCAACATATCGAAAATCGCCCCGCCGTTATTATTAATGATAAAAAGAATCGTCGGTTGGGTGATTTTTTTGAATAACGCCAGCGAGTTTAAATCATACAGCGCGGACGTGTCGCCCACTAACGCGACCACCGGCTGATTGGAACCGATACCGATGCCGGCGACGGTGGCAAGTAAACCGTCGATGCCACTgg > 1:75339/1‑251 (MQ=255) aTTCC‑GTATGGTGCGGCATACGG‑TAGAATTTGTCTTTCACTTCCGCATCCACCGGCAACATATCGAAAATCGCCCCGCCGTTATTATTAATGATAAAAAGAATCGTCGGTTGGGTGATTTTTTTGAATAACGCCAGCGAGTTTAAATCATACAGCGCGGACGTGTCGCCCACTAACGCGACCACCGGCTGATTGGAACCGATACCGATGCCGGCGACGGTGGCAAGTAAACCGTCGATGCCACTgctgtc > 1:235099/1‑245 (MQ=255) tCC‑GTATGGTGCGGCATACGG‑TAGAATTTGTCTTTCACTTCCGCATCCACCGGCAACATATCGAAAATCGCCCCGCCGTTATTATTAATGATAAAAAGAATCGTCGGTTGGGTGATTTTTTTGAATAACGCCAGCGAGTTTAAATCATACAGCGCGGACGTGTCGCCCACTAACGCGACCACCGGCTGATTGGAACCGATACCGATGCCGGCGACGGTGGCAAGTAAACCGTCGATGCCACTGGCGCcgcg > 1:883551/1‑251 (MQ=255) | CAAATTATCATGAAATAATTTGCTTTTGATGGTGTTATTTATTCGCCGACGACAGCATAGCTGATTTGTTCGATTAGGCGTTTATAAACGGTGCTGCCGTCATTCGGCGCCACTTTGATTTCGATGATGGTGGCTTCACGGCGTGAATAGGCTTGTTTCAGTACGGAACTCAAATCCGCCCA‑GGTGTAAGGGCGCGCATATTTT‑AAATCGAACATGGACGCCACTTGGG‑AAAATTCC‑GTGTGGTGCGGCATACGG‑TAGAATTTGTCTTTCACTTCCGCATCCACCGGCAACATATCGAAAATCGCCCCGCCGTTATTATTAATGATAAAAAGAATCGTCGGTTGGGTGATTTTTTTGAATAACGCCAGCGAGTTTAAATCATACAGCGCGGACGTGTCGCCCACTAACGCGACCACCGGCTGATTGGAACCGATACCGATGCCGGCGACGGTGGCAAGTAAACCGTCGATGCCACTGGCGCCGCG > 1/185611‑186095 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |