Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,357,874	A→G	I160M (ATA→ATG)	unknown →	ABC transporter, permease protein 1 (cluster 1, maltose/g3p/polyamine/iron) / ABC transporter, permease protein 2 (cluster 1, maltose/g3p/polyamine/iron)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,357,874	0	A	G	100.0%	213.5 / NA	74	I160M (ATA→ATG)	unknown	ABC transporter, permease protein 1 (cluster 1, maltose/g3p/polyamine/iron) / ABC transporter, permease protein 2 (cluster 1, maltose/g3p/polyamine/iron)
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (36/38);  total (36/38)

ATAGAGTTCATTTTATCCTGTATAATTTTTGGATTTGTGATGGTCTCAGCCACAATTGCAAAAATCAGTTTGGGCTATTCTATTTTTGTTCTCACCATAGCACTAATCGGAGTTTCTACGAATGCACTTGCCAAAATGAAAGTCATGCAAAGTGATAAATTCATCATTGGCTCTTTAATTAGCATTGTCCTATTAATATTCTTTTTTATCGTTTATCCAACATTGGCGATCTTTATATCAATGTTCTATAAAGGGACAGATTTTGTTCCCGGGCAAGTCATAGATATTATAAAGCAGCCTTATGTTGTCAGAATCATCCTAAACTCACTGTCTGTTGCCGGCACAATAGGCGTACTCGCTACAGCATTTGGTTTGATATTCGCGTTATACACAACAAGAATTGCCAAACGTAGTGCCGTAATAGGTAAAATATTCTCTATATTACCTATTGTCACCCCACCATTTGTAGTTGGATTAGGG  >  1/1357638‑1358117                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                    aTAGAGTTCATTTTATCCTGTATAATTTTTGGATTTGTGATGGTCTCAGCCACAATTGCAAAAATCAGTTTGGGCTATTCTATTTTTGTTCTCACCATAGCACTAATCGGAGTTTCTACGAATGCACTTGCCAAAATGAAAGTCATGCAAAGTGATAAATTCATCATTGGCTCTTTAATTAGCATTGTCCTATTAATATTCTTTTTTATCGTTTATCCAACATTGGCGATCTTTATGTCAATGTTCTATaa                                                                                                                                                                                                                                       <  1:493824/251‑1 (MQ=255) aTAGAGTTCATTTTATCCTGTATAATTTTTGGATTTGTGATGGTCTCAGCCACAATTGCAAAAATCAGTTTGGGCTATTCTATTTTTGTTCTCACCATAGCACTAATCGGAGTTTCTACGAATGCACTTGCCAAAATGAAAGTCATGCAAAGTGATAAATTCATCATTGGCTCTTTAATTAGCATTGTCCTATTAATATTCTTTTTTATCGTTTATCCAACATTGGCGATCTTTATGTCAATGTTCTATaa                                                                                                                                                                                                                                       <  1:640852/251‑1 (MQ=255)     agTTCATTTTATCCTGTATAATTTTTGGATTTGTGATGGTCTCAGCCACAATTGCAAAAATCAGTTTGGGCTATTCTATTTTTGTTCTCACCATAGCACTAATCGGAGTTTCTACGAATGCACTTGCCAAAATGAAAGTCATGCAAAGTGATAAATTCATCATTGGCTCTTTAATTAGCATTGTCCTATTAATATTCTTTTTTATCGTTTATCCAACATTGGCGATCTTTATGTCAATGTTCTATAAAgg                                                                                                                                                                                                                                    <  1:595738/250‑1 (MQ=255)       ttCATTTTATCCTGTATAATTTTTGGATTTGTGATGGTCTCAGCCACAATTGCAAAAATCAGTTTGGGCTATTCTATTTTTGTTCTCACCATAGCACTAATCGGAGTTTCTACGAATGCACTTGCCAAAATGAAAGTCATGCAAAGTGATAAATTCATCATTGGCTCTTTAATTAGCATTGTCCTATTAATATTCTTTTTTATCGTTTATCCAACATTGGCGATCTTTATGTCAATGTTCTATAAAGGGAc                                                                                                                                                                                                                                 <  1:844769/251‑1 (MQ=255)                    gTATAATTTTTGGATTTGTGATGGTCTCAGCCACAATTGCAAAAATCAGTTTGGGCTATTCTATTTTTGTTCTCACCATAGCACTAATCGGAGTTTCTACGAATGCACTTGCCAAAATGAAAGTCATGCAAAGTGATAAATTCATCATTGGCTCTTTAATTAGCATTGTCCTATTAATATTCTTTTTTATCGTTTATCCAACATTGGCGATCTTTATGTCAATGTTCTATAAAGGGACAGATTTTGTTcc                                                                                                                                                                                                                     >  1:815667/1‑250 (MQ=255)                    gTATAATTTTTGGATTTGTGATGGTCTCAGCCACAATTGCAAAAATCAGTTTGGGCTATTCTATTTTTGTTCTCACCATAGCACTAATCGGAGTTTCTACGAATGCACTTGCCAAAATGAAAGTCATGCAAAGTGATAAATTCATCATTGGCTCTTTAATTAGCATTGTCCTATTAATATTCTTTTTTATCGTTTATCCAACATTGGCGATCTTTATGTCAATGTTCTATAAAGGGACAGATTTTGTTcc                                                                                                                                                                                                                     >  1:24961/1‑250 (MQ=255)                                           gTCTCAGCCACAATTGCAAAAATCAGTTTGGGCTATTCTATTTTTGTTCTCACCATAGCACTAATCGGAGTTTCTACGAATGCACTTGCCAAAATGAAAGTCATGCAAAGTGATAAATTCATCATTGGCTCTTTAATTAGCATTGTCCTATTAATATTCTTTTTTATCGTTTATCCAACATTGGCGATCTTTATGTCAATGTTCTATAAAGGGACAGATTTTGTTCCCGGGCAAGTCATAGat                                                                                                                                                                                                     >  1:436845/1‑243 (MQ=255)                                             ctcAGCCACAATTGCAAAAATCAGTTTGGGCTATTCTATTTTTGTTCTCACCATAGCACTAATCGGAGTTTCTACGAATGCACTTGCCAAAATGAAAGTCATGCAAAGTGATAAATTCATCATTGGCTCTTTAATTAGCATTGTCCTATTAATATTCTTTTTTATCGTTTATCCAACATTGGCGATCTTTATGTCAATGTTCTATAAAGGGACAGATTTTGTTCCCGGGCAAGTCATAGATATTATAAagc                                                                                                                                                                                           <  1:547810/251‑1 (MQ=255)                                               cAGCCACAATTGCAAAAATCAGTTTGGGCTATTCTATTTTTGTTCTCACCATAGCACTAATCGGAGTTTCTACGAATGCACTTGCCAAAATGAAAGTCATGCAAAGTGATAAATTCATCATTGGCTCTTTAATTAGCATTGTCCTATTAATATTCTTTTTTATCGTTTATCCAACATTGGCGATCTTTATGTCAATGTTCTATAAAgg                                                                                                                                                                                                                                    <  1:166191/208‑1 (MQ=255)                                                       aTTGCAAAAATCAGTTTGGGCTATTCTATTTTTGTTCTCACCATAGCACTAATCGGAGTTTCTACGAATGCACTTGCCAAAATGAAAGTCATGCAAAGTGATAAATTCATCATTGGCTCTTTAATTAGCATTGTCCTATTAATATTCTTTTTTATCGTTTATCCAACATTGGCGATCTTTATGTCAATGTTCTATAAAGGGACAGATTTTGTTCCCGGGCAAGTCATAGATATTATAAAGCAGCCTTAtgt                                                                                                                                                                                 <  1:427902/251‑1 (MQ=255)                                                         tGCAAAAATCAGTTTGGGCTATTCTATTTTTGTTCTCACCATAGCACTAATCGGAGTTTCTACGAATGCACTTGCCAAAATGAAAGTCATGCAAAGTGATAAATTCATCATTGGCTCTTTAATTAGCATTGTCCTATTAATATTCTTTTTTATCGTTTATCCAACATTGGCGATCTTTATGTCAATGTTCTATAAAGGGACAGATTTTGTTCCCGGGCAAGTCATAGATATTATAAAGCAGCCTTAtgttg                                                                                                                                                                               <  1:470125/251‑1 (MQ=255)                                                                   aGTTTGGGCTATTCTATTTTTGTTCTCACCATAGCACTAATCGGAGTTTCTACGAATGCACTTGCCAAAATGAAAGTCATGCAAAGTGATAAATTCATCATTGGCTCTTTAATTAGCATTGTCCTATTAATATTCTTTTTTATCGTTTATCCAACATTGGCGATCTTTATGTCAATGTTCTATAAAGGGACAGATTTTGTTCCCGGGCAAGTCATAGATATTATAAAGCAGCCTTATGTTGTCAGAatcat                                                                                                                                                                     >  1:154764/1‑251 (MQ=255)                                                                             attctattTTTGTTCTCACCATAGCACTAATCGGAGTTTCTACGAATGCACTTGCCAAAATGAAAGTCATGCAAAGTGATAAATTCATCATTGGCTCTTTAATTAGCATTGTCCTATTAATATTCTTTTTTATCGTTTATCCAACATTGGCGATCTTTATGTCAATGTTCTATAAAGGGACAGATTTTGTTCCCGGGCAAGTCATAGATATTATAAagc                                                                                                                                                                                           >  1:745707/1‑219 (MQ=255)                                                                             attctattTTTGTTCTCACCATAGCACTAATCGGAGTTTCTACGAATGCACTTGCCAAAATGAAAGTCATGCAAAGTGATAAATTCATCATTGGCTCTTTAATTAGCATTGTCCTATTAATATTCTTTTTTATCGTTTATCCAACATTGGCGATCTTTATGTCAATGTTCTATAAAGGGACAGATTTTGTTCCCGGGCAAGTCATAGATATTATAAAGCAGCCTTATGTTGTCAGAATCATCCTAAACTCa                                                                                                                                                           >  1:401366/1‑251 (MQ=255)                                                                                  attTTTGTTCTCACCATAGCACTAATCGGAGTTTCTACGAATGCACTTGCCAAAATGAAAGTCATGCAAAGTGATAAATTCATCATTGGCTCTTTAATTAGCATTGTCCTATTAATATTCTTTTTTATCGTTTATCCAACATTGGCGATCTTTATGTCAATGTTCTATAAAGGGACAGATTTTGTTCCCGGGCAAGTCATAGat                                                                                                                                                                                                     <  1:802999/204‑1 (MQ=255)                                                                                  attTTTGTTCTCACCATAGCACTAATCGGAGTTTCTACGAATGCACTTGCCAAAATGAAAGTCATGCAAAGTGATAAATTCATCATTGGCTCTTTAATTAGCATTGTCCTATTAATATTCTTTTTTATCGTTTATCCAACATTGGCGATCTTTATGTCAATGTTCTATAAAGGGACAGATTTTGTTCCCGGGCAAGTCATAGATATTATAAAGCAGCCTTATGTTGTCAGAATCATCCTAAACTCa                                                                                                                                                           >  1:217681/1‑246 (MQ=255)                                                                                        gTTCTCACCATAGCACTAGTCGGAGTTTCTACGAATGCACTTGCCAAAATGAAAGTCATGCAAAGTGATAAATTCATCATTGGCTCTTTAATTAGCATTGTCCTATTAATATTCTTTTTTATCGTTTATCCAACATTGGCGATCTTTATGTCAATGTTCTATAAAGGGACAGATTTTGTTCCCGGGCAAGTCATAGa                                                                                                                                                                                                      <  1:811904/197‑1 (MQ=255)                                                                                               ccATAGCACTAATCGGAGTTTCTACGAATGCACTTGCCAAAATGAAAGTCATGCAAAGTGATAAATTCATCATTGGCTCTTTAATTAGCATTGTCCTATTAATATTCTTTTTTATCGTTTATCCAACATTGGCGATCTTTATGTCAATGTTCTATAAAGGGACAGATTTTGTTCCCGGGCAAGTCATAGATATTATaa                                                                                                                                                                                              >  1:682223/1‑198 (MQ=255)                                                                                               ccATAGCACTAATCGGAGTTTCTACGAATGCACTTGCCAAAATGAAAGTCATGCAAAGTGATAAATTCATCATTGGCTCTTTAATTAGCATTGTCCTATTAATATTCTTTTTTATCGTTTATCCAACATTGGCGATCTTTATGTCAATGTTCTATAAAGGGACAGATTTTGTTCCCGGGCAAGTCATAGATATTATAAAGCAGCCTTAtgt                                                                                                                                                                                 >  1:363236/1‑211 (MQ=255)                                                                                               ccATAGCACTAATCGGAGTTTCTACGAATGCACTTGCCAAAATGAAAGTCATGCAAAGTGATAAATTCATCATTGGCTCTTTAATTAGCATTGTCCTATTAATATTCTTTTTTATCGTTTATCCAACATTGGCGATCTTTATGTCAATGTTCTATAAAGGGACAGATTTTGTTCCCGGGCAAGTCATAGATATTATAAAGCAGCCTTAtgt                                                                                                                                                                                 >  1:680974/1‑211 (MQ=255)                                                                                               ccATAGCACTAATCGGAGTTTCTACGAATGCACTTGCCAAAATGAAAGTCATGCAAAGTGATAAATTCATCATTGGCTCTTTAATTAGCATTGTCCTATTAATATTCTTTTTTATCGTTTATCCAACATTGGCGATCTTTATGTCAATGTTCTATAAAGGGACAGATTTTGTTCCCGGGCAAGTCATAGATATTATAAAGCAGCCTTATGTTGTCAGAATCATCCTAAACTCACTGTCTGTTGCCGGcaca                                                                                                                                         >  1:203133/1‑251 (MQ=255)                                                                                               ccATAGCACTAATCGGAGTTTCTACGAATGCACTTGCCAAAATGAAAGTCATGCAAAGTGATAAATTCATCATTGGCTCTTTAATTAGCATTGTCCTATTAATATTCTTTTTTATCGTTTATCCAACATTGGCGATCTTTATGTCAATGTTCTATAAAGGGACAGATTTTGTTCCCGGGCAAGTCATAGATATTATAAAGCAGCCTTATGTTGTCAGAATCATCCTAAACTCACTGTCTGTTGCCGGcaca                                                                                                                                         >  1:156517/1‑251 (MQ=255)                                                                                                    gCACTAATCGGAGTTTCTACGAATGCACTTGCCAAAATGAAAGTCATGCAAAGTGATAAATTCATCATTGGCTCTTTAATTAGCATTGTCCTATTAATATTCTTTTTTATCGTTTATCCAACATTGGCGATCTTTATGTCAATGTTCTATAAAGGGACAGATTTTGTTCCCGGGCAAGTCATAGATATTATAAAGCAGCCTTATGTTGTCAGAATCATCCTAAACTCACTGTCTGTTGCCGGCACAATAgg                                                                                                                                    >  1:291345/1‑251 (MQ=255)                                                                                                     cACTAATCGGAGTTTCTACGAATGCACTTGCCAAAATGAAAGTCATGCAAAGTGATAAATTCATCATTGGCTCTTTAATTAGCATTGTCCTATTAATATTCTTTTTTATCGTTTATCCAACATTGGCGATCTTTATGTCAATGTTCTATAAAGGGACAGATTTTGTTCCCGGGCAAGTCATAGATATTATAAAGCAGCCTTATGTTGTCAGAATCATCCTAAACTCACTGTCTGTTGCCGGCACAATAgg                                                                                                                                    <  1:632593/250‑1 (MQ=255)                                                                                                     cACTAATCGGAGTTTCTACGAATGCACTTGCCAAAATGAAAGTCATGCAAAGTGATAAATTCATCATTGGCTCTTTAATTAGCATTGTCCTATTAATATTCTTTTTTATCGTTTATCCAACATTGGCGATCTTTATGTCAATGTTCTATAAAGGGACAGATTTTGTTCCCGGGCAAGTCATAGATATTATAAAGCAGCCTTATGTTGTCAGAATCATCCTAAACTCACTGTCTGTTGCCGGCACAATAgg                                                                                                                                    <  1:417602/250‑1 (MQ=255)                                                                                                      aCTAATCGGAGTTTCTACGAATGCACTTGCCAAAATGAAAGTCATGCAAAGTGATAAATTCATCATTGGCTCTTTAATTAGTATTGTCCTATTAATATTCTTTTTTATCGTTTATCCAACATTGGCGATCTTTATGTCAATGTTCTATAAAGGGACAGATTTTGTTCCCGGGCAAGTCATAGATATTATAAAGCAGCCTTATGTTGTCAGAATCATCCTAAACTCACTGTCTGTTGCCGGCACAATAGGCg                                                                                                                                  <  1:432489/251‑1 (MQ=255)                                                                                                         aaTCGGAGTTTCTACGAATGCACTTGCCAAAATGAAAGTCATGCAAAGTGATAAATTCATCATTGGCTCTTTAATTAGCATTGTCCTATTAATATTCTTTTTTATCGTTTATCCAACATTGGCGATCTTTATGTCAATGTTCTATAAAGGGACAGATTTTGTTCCCGGGCAAGTCATAGATATTATAAagcag                                                                                                                                                                                         <  1:106936/193‑1 (MQ=255)                                                                                                                                 tGCCAAAATGAAAGTCATGCAAAGTGATAAATTCATCATTGGCTCTTTAATTAGCATTGTCCTATTAATATTCTTTTTTATCGTTTATCCAACATTGGCGATCTTTATGTCAATGTTCTATAAAGGGACAGATTTTGTTCCCGGGCAAGTCATAGATATTATAAAGCAGCCTTATGTTGTCAGAATCATCCTAAACTCACTGTCTGTTGCCGGCACAATAGGCGTACTCGCTACAGCATTTGGTTTGatat                                                                                                       >  1:568024/1‑251 (MQ=255)                                                                                                                                       aaTGAAAGTCATGCAAAGTGATAAATTCATCATTGGCTCTTTAATTAGCATTGTCCTATTAATATTCTTTTTTATCGTTTATCCAACATTGGCGATCTTTATGTCAATGTTCTATAAAGGGAc                                                                                                                                                                                                                                 >  1:858506/1‑123 (MQ=255)                                                                                                                                       aaTGAAAGTCATGCAAAGTGATAAATTCATCATTGGCTCTTTAATTAGCATTGTCCTATTAATATTCTTTTTTATCGTTTATCCAACATTGGCGATCTTTATGTCAATGTTCTATAAAGGGACAGATTTTGTTCCCGGGCAAGTCATAGATATTATAAAGCAGCCTTATGTTGTCAGAATCATCCTAAACTCACTGTCTGTTGCCGGCACAATAGGCGTACTCGCTACAGCATTTGGTTTGATATTCGCGt                                                                                                 >  1:378077/1‑251 (MQ=255)                                                                                                                                              gTCATGCAAAGTGATAAATTCATCATTGGCTCTTTAATTAGCATTGTCCTATTAATATTCTTTTTTATCGTTTATCCAACATTGGCGATCTTTATGTCAATGTTCTATAAAGGGACAGATTTTGTTCCCGGGCAAGTCATAGATATTATAAAGCAGCCTTATGTTGTCAGAATCATCCTAAACTCACTGTCTGTTGCCGGCACAATAGGCGTACTCGCTACAGCAt                                                                                                                   >  1:401328/1‑226 (MQ=255)                                                                                                                                                 aTGCAAAGTGATAAATTCATCATTGGCTCTTTAATTAGCATTGTCCTATTAATATTCTTTTTTATCGTTTATCCAACATTGGCGATCTTTATGTCAATGTTCTATAAAGGGACAGATTTTGTTCCCGGGCAAGTCATAGATATTATAAAGCAGCCTTATGTTGTCAGAATCATCCTAAACTCACTGTCTGTTGCCGGCACAATAGGCGTACTCGCTACAGCATTTGGTTTGATATTCGCGTTATACacaac                                                                                       <  1:879370/251‑1 (MQ=255)                                                                                                                                                                 tcatcaTTGGCTCTTTAATTAGCATTGTCCTATTAATATTCTTTTTTATCGTTTATCCAACATTGGCGATCTTTATGTCAATGTTCTATAAAGGGACAGATTTTGTTCCCGGGCAAGTCATAGATATTATAAAGCAGCCTTATGTTGTCAGAATCATCCTAAACTCACTGTCTGTTGCCGGCACAATAGGCGTACTCGCTACAGCATTTGGTTTGATATTCGCGTTATACACAACAAGAATTGCCAAACGt                                                                       <  1:719910/251‑1 (MQ=255)                                                                                                                                                                             ctTTAATTAGCATTGTCCTATTAATATTCTTTTTTATCGTTTATCCAACATTGGCGATCTTTATGTCAATGTTCTATAAAGGGACAGATTTTGTTCCCGGGCAAGTCATAGATATTATAAAGCAGCCTTATGTTGTCAGAATCATCCTAAACTCACTGTCTGTTGCCGGCACAATAGGCGTACTCGCTACAGCATTTGGTTTGATATTCGCGTTATACACAACAAGAATTGCCAAACGTAGTGCCGTAATa                                                           <  1:529514/251‑1 (MQ=255)                                                                                                                                                                               ttAATTAGCATTGTCCTATTAATATTCTTTTTTATCGTTTATCCAACATTGGCGATCTTTATGTCAATGTTCTATAAAGGGACAGATTTTGTTCCCGGGCAAGTCATAGAtattat                                                                                                                                                                                                >  1:839227/1‑116 (MQ=255)                                                                                                                                                                               ttAATTAGCATTGTCCTATTAATATTCTTTTTTATCGTTTATCCAACATTGGCGATCTTTATGTCAATGTTCTATAAAGGGACAGATTTTGTTCCCGGGCAAGTCATAGATATTATAAAGCAGCCTTAtgt                                                                                                                                                                                 <  1:387737/131‑1 (MQ=255)                                                                                                                                                                                tAATTAGCATTGTCCTATTAATATTCTTTTTTATCGTTTATCCAACATTGGCGATCTTTATGTCAATGTTCTATAAAGGGACAGATTTTGTTCCCGGGCAAGTCATAGATATTATAAAGCAGCCTTATGTTGTCAGAATCATCCTAAACTCACTGTCTGTTGCCGGCACAATAGGCGTACTCGCTACAGCATTTGGTTTGATATTCGCGTTATACACAACAAGAATTGCCAAACGTAGTGCCGTAATAGGt                                                        <  1:204325/251‑1 (MQ=255)                                                                                                                                                                                 aaTTAGCATTGTCCTATTAATATTCTTTTTTATCGTTTATCCAACATTGGCGATCTTTATGTCAATGTTCTATAAAGGGACAGAttt                                                                                                                                                                                                                           <  1:102786/87‑1 (MQ=255)                                                                                                                                                                                 aaTTAGCATTGTCCTATTAATATTCTTTTTTATCGTTTATCCAACATTGGCGATCTTTATGTCAATGTTCTATAAAGGGACAGATTTTGTTCCCGGGCAAGTCATAGATATTATAAAGCAGCCTTATGTTGTCAGAATCATCCTAAACTc                                                                                                                                                            >  1:850007/1‑150 (MQ=255)                                                                                                                                                                                 aaTTAGCATTGTCCTATTAATATTCTTTTTTATCGTTTATCCAACATTGGCGATCTTTATGTCAATGTTCTATAAAGGGACAGATTTTGTTCCCGGGCAAGTCATAGATATTATAAAGCAGCCTTATGTTGTCAGAATCATCCTAAACTCACTGTCTGTTGCCGGCACAGTAGGCGTACTCGCTACAGCATTTGGt                                                                                                              >  1:456461/1‑196 (MQ=255)                                                                                                                                                                                      gCATTGTCCTATTAATATTCTTTTTTATCGTTTATCCAACATTGGCGATCTTTATGTCAATGTTCTATAAAGGGACAGATTTTGTTCCCGGGCAAGTCATAGat                                                                                                                                                                                                     >  1:672576/1‑104 (MQ=255)                                                                                                                                                                                      gCATTGTCCTATTAATATTCTTTTTTATCGTTTATCCAACATTGGCGATCTTTATGTCAATGTTCTATAAAGGGACAGATTTTGTTCCCGGGCAAGTCATAGATATTATAAAGCAGCCTTATGTTGTCAGAATCATCCTAAACTCACTGTCTGTTGCCGGCACAATAGGCGTACTCGCTACAGCATTTGGTTTGATATTCGCGTTATac                                                                                            >  1:39903/1‑209 (MQ=255)                                                                                                                                                                                      gCATTGTCCTATTAATATTCTTTTTTATCGTTTATCCAACATTGGCGATCTTTATGTCAATGTTCTATAAAGGGACAGATTTTGTTCCCGGGCAAGTCATAGATATTATAAAGCAGCCTTATGTTGTCAGAATCATCCTAAACCCACTGTCTGTTGCCGGCACAATAGGCGTACTCGCTACAGCATTTGGTTTGATATTCGCGTTATACACAACAAGAATTGCCAAACGTAGTGCCGTAATAGGTAAAata                                                  >  1:853727/1‑251 (MQ=255)                                                                                                                                                                                           gTCCTATTAATATTCTTTTTTATCGTTTATCCAACATTGGCGATCTTTATGTCAATGTTCTATaa                                                                                                                                                                                                                                       >  1:283034/1‑65 (MQ=255)                                                                                                                                                                                           gTCCTATTAATATTCTTTTTTATCGTTTATCCAACATTGGCGATCTTTATGTCAATGTTCTATAAAGGGACAGATTTTGTTCCCGGGCAAGTCATAGATATTATAAAGCAGCCTTATGTTGTCAGAATCATCCTAAACTCACTGTCTGTTGCCGGCACAATAGGCGTACTCGCTACAGCAt                                                                                                                   <  1:430582/181‑1 (MQ=255)                                                                                                                                                                                           gTCCTATTAATATTCTTTTTTATCGTTTATCCAACATTGGCGATCTTTATGTCAATGTTCTATAAAGGGACAGATTTTGTTCCCGGGCAAGTCATAGATATTATAAAGCAGCCTTATGTTGTCAGAATCATCCTAAACTCACTGTCTGTTGCCGGCACAATAGGCGTACTCGCTACAGCATTTGGTTTGATATTCGCGTTATACACAACAAGaa                                                                                  <  1:488609/214‑1 (MQ=255)                                                                                                                                                                                           gTCCTATTAATATTCTTTTTTATCGTTTATCCAACATTGGCGATCTTTATGTCAATGTTCTATAAAGGGACAGATTTTGTTCCCGGGCAAGTCATAGATATTATAAAGCAGCCTTATGTTGTCAGAATCATCCTAAACTCACTGTCTGTTGCCGGCACAATAGGCGTACTCGCTACAGCATTTGGTTTGATATTCGCGTTATACACAACAAGAATTGCCAAACGTAGTGCCGTAATAGGTAAAATATtct                                              >  1:155793/1‑250 (MQ=255)                                                                                                                                                                                             ccTATTAATATTCTTTTTTATCGTTTATCCAACATTGGCGATCTTTATGTCAATGTTCTATAAAGGGACAGATTTTGTTCCCGGGCAAGTCATAGATATTATAAAGCAGCCTTATGTTGTCAGAATCATCCTAAACTCACTGTCTGTTGCCGGCACAATAGGCGTACTCGCTACAGCATTTGGTTTGATATTCGCGTTATACACAACAAGAATTGCCAAACGTAGTGCCGTAATAGGTAAAat                                                   <  1:540342/243‑1 (MQ=255)                                                                                                                                                                                              cTATTAATATTCTTTTTTATCGTTTATCCAACATTGGCGATCTTTATGTCAATGTTCTATAAAGGGACAGATTTTGTTCCCGGGCAAGTCATAGATATTATAAAGCAGCCTTATGTTGTCAGAATCATCCTAAACTCACTGTCTGTTGCCGGCACAATAGGCGTACTCGCTACAGCATTTGGTTTGATATTCGCGTTATACACAACAAGAATTGCCAAACGTAGTGCCGTAATAGGTAAAatat                                                 <  1:602608/244‑1 (MQ=255)                                                                                                                                                                                              cTATTAATATTCTTTTTTATCGTTTATCCAACATTGGCGATCTTTATGTCAATGTTCTATAAAGGGACAGATTTTGTTCCCGGGCAAGTCATAGATATTATAAAGCAGCCTTATGTTGTCAGAATCATCCTAAACTCACTGTCTGTTGCCGGCACAATAGGCGTACTCGCTACAGCATTTGGTTTGATATTCGCGTTATACACAACAAGAATTGCCAAACGTAGTGCCGTAATAGGTAAAATATTCTCtat                                          <  1:59845/251‑1 (MQ=255)                                                                                                                                                                                                aTTAATATTCTTTTTTATCGTTTATCCAACATTGGCGATCTTTATGTCAATGTTCTATAAAGGGACAGATTTTGTTCCCGGGCAAGTCATAGat                                                                                                                                                                                                     >  1:614022/1‑94 (MQ=255)                                                                                                                                                                                                aTTAATATTCTTTTTTATCGTTTATCCAACATTGGCGATCTTTATGTCAATGTTCTATAAAGGGACAGATTTTGTTCCCGGGCAAGTCATAGATATTATAAAGCAGCCTTATGTTGTCAGAATCATCCTAAACTCTCTGTCTGTTGCCGGCACAATAGGCGTACTCGCTACAGCATTTGGTTTGATATTCGCGTTATTCACAACAAGAATTGCCAAACGTAGTGc                                                                  <  1:152972/225‑1 (MQ=255)                                                                                                                                                                                                aTTAATATTCTTTTTTATCGTTTATCCAACATTGGCGATCTTTATGTCAATGTTCTATAAAGGGACAGATTTTGTTCCCGGGCAAGTCATAGATATTATAAAGCAGCCTTATGTTGTCAGAATCATCCTAAACTCACTGTCTGTTGCCGGCACAATAGGCg                                                                                                                                  >  1:768511/1‑161 (MQ=255)                                                                                                                                                                                                aTTAATATTCTTTTTTATCGTTTATCCAACATTGGCGATCTTTATGTCAATGTTCTATAAAGGGACAGATTTTGTTCCCGGGCAAGTCATAGATATTATAAAGCAGCCTTATGTTGTCAGAATCATCCTAAACTCACTGTCTGTTGCCGGCACAATAGGCGTACTCGCTACAGCATTTGGTTTGATATTCGCGTTATACACAACAAGAATTGCCAAACGTAGTGCCGTAATAGGTAAAATATTCTCTatat                                        >  1:719593/1‑251 (MQ=255)                                                                                                                                                                                                 ttAATATTCTTTTTTATCGTTTATCCAACATTGGCGATCTTTATGTCAATGTTCTATAAAGGGACAGATTTTGTTCCCGGGCAAGTCATAGATATTATAAAGCAGCCTTATGTTGTCAGAATCATCCTAAACTCACTGTCTGTTGCCGGCACAATAGGCGTACTCGCTACAGCATTTGGTTTGATATTCGCGTTATACACAACAAGAATTGCCAAACGTAGTGCCGTAATAGGTAAAATATTCTCTATAtt                                       <  1:576431/251‑1 (MQ=255)                                                                                                                                                                                                    atatTCTTTTTTATCGTTTATCCAACATTGGCGATCTTTATGTCAATGTTCTATAAAGGGACAGATTTTGTTCCCGGGCAAGTCATAGATATTATAAAGCAGCCTTATGTTGTCAGAATCATCCTAAACTCACTGTCTGTTGCCGGCACAATAGGCGTACTCGCTACAGCATTTGGTTTGATATTCGCGTTATACACAACAAGAATTGCCAAACGTAGTGCCGTAATAGGTAAAATATTCTCTATATTAcc                                    <  1:770825/251‑1 (MQ=255)                                                                                                                                                                                                     tatTCTTTTTTATCGTTTATCCAACATTGGCGATCTTTATGTCAATGTTCTATAAAGGGACAGATTTTGTTCCCGGGCAAGTCATAGATATTATAAAGCAGCCTTATGTTGTCAGAATCATCCTAAACTCACTGTCTGTTGCCGGCACAATAGGCGTACTCGCTACAGCATTTGGTTTGATATTCGCGTTATACACAACAAGAATTGCCAAACGTAGTGCCGTAATAGGTAAAATATTCTCTATATTACCt                                   <  1:825505/251‑1 (MQ=255)                                                                                                                                                                                                     tatTCTTTTTTATCGTTTATCCAACATTGGCGATCTTTATGTCAATGTTCTATAAAGGGACAGATTTTGTTCCCGGGCAAGTCATAGATATTATAAAGCAGCCTTATGTTGTCAGAATCATCCTAAACTCACTGTCTGTTGCCGGCACAATAGGCGTACTCGCTACAGCATTTGGTTTGATATTCGCGTTATACACAACAAGAATTGCCAAACGTAGTGCCGTAATAGGTAAAATATTCTCTATATTACCt                                   <  1:5325/251‑1 (MQ=255)                                                                                                                                                                                                     tatTCTTTTTTATCGTTTATCCAACATTGGCGATCTTTATGTCAATGTTCTATAAAGGGACAGATTTTGTTCCCGGGCAAGTCATAGATATTATAAAGCAGCCTTATGTTGTCAGAATCATCCTAAACTCACTGTCTGTTGCCGGCACAATAGGCGTACTCGCTACAGCATTTGGTTTGATATTCGCGTTATACACAACAAGAATTGCCAAACGTAGTGCCGTAATAGGTAAAATATTCTCTATATTACCt                                   <  1:68600/251‑1 (MQ=255)                                                                                                                                                                                                     tatTCTTTTTTATCGTTTATCCAACATTGGCGATCTTTATGTCAATGTTCTATAAAGGGACAGATTTTGTTCCCGGGCAAGTCATAGATATTATAAAGCAGCCTTATGTTGTCAGAATCATCCTAAACTCACTGTCTGTTGCCGGCACAATAGGCGTACTCGCTACAGCATTTGGTTTGATATTCGCGTTATACACAACAAGAATTGCCAAACGTAGTGCCGTAATAGGTAAAATATTCTCTATATTACCt                                   <  1:414875/251‑1 (MQ=255)                                                                                                                                                                                                           tttttATCGTTTATCCAACATTGGCGATCTTTATGTCAATGTTCTATAACGGGACAGATTTTGTTCCCGGGCAAGTCATAGATATTATAAAGCAGCCTTATGTTGTCAGAATCATCCTAAACTCACTGTCTGTTGCCGGCACAATAGGCGTACTCGCTACAGCATTTGGTTTGATATTCGCGTTATACACAACAAGAATTGCCAAACGTAGTGCCGTAATAGGTAAAATATTCTCTATATTACCTATTGt                              >  1:295530/1‑250 (MQ=255)                                                                                                                                                                                                           tttttATCGTTTATCCAACATTGGCGATCTTTATGTCAATGTTCTATAAAGGGACAGATTTTGTTCCCGGGCAAGTCATAGATATTATAAAGCAGCCTTATGTTGTCAGAATCATCCTAAACTCACTGTCTGTTGCCGGCACAATAGGCg                                                                                                                                  <  1:322868/150‑1 (MQ=255)                                                                                                                                                                                                              ttATCGTTTATCCAACATTGGCGATCTTTATGTCAATGTTCTATAAAGGGACAGATTTTGTTCCCGGGCAAGTCATAGATATTATAAAGCAGCCTTATGTTGTCGGAATCATCCTAAACTCACTGTCTGTTGCCGGCACAATAGGCGTACTCGCTACAGCATTTGGTTTGATATTCGCGTTATACACAACAAGAATTGCCAAACGTAGTGCCGTAATAGGTAAAATATTCTCTATATTACCTATTGTCAcc                          <  1:610833/251‑1 (MQ=255)                                                                                                                                                                                                                   gTTTATCCAACATTGGCGATCTTTATGTCAATGTTCTATAAAGGGACAGATTTTGTTCCCGGGCAAGTCATAGATATTATAAAGCAGCCTTATGTTGTCAGAATCATCCTAAACTCACTGTCTGTTGCCGGCACAATAGGCGTACTCGCTACAGCATTTGGTTTGATATTCGCGTTATACACAACAAGAATTGCCAAACGTAGTGCCGTAATAGGTAAAatat                                                 <  1:657998/223‑1 (MQ=255)                                                                                                                                                                                                                       aTCCAACATTGGCGATCTTTATGTCAATGTTCTATAAAGGGACAGATTTTGTTCCCGGGCAAGTCATAGATATTATAAAGCAGCCTTATGTTGTCAGAATCATCCTAAACTCACTGTCTGTTGCCGGCACAATAGGCGTACTCGCTACAGCATTTGGTTTGATATTCGCGTTATACACAACAAGAATTGCCAAACGTAGTGCCGTAATAGGTAAAATATTCTCTATATTACCTATTGTCACCCCACCAttt                 >  1:832310/1‑251 (MQ=255)                                                                                                                                                                                                                           aaCATTGGCGATCTTTATGTCAATGTTCTATAAAGGGACAGATTTTGTTCCCGGGCAAGTCATAGATATTATAAAGCAGCCTTATGTTGTCAGAATCATCCTAAACTCACTGTCTGTTGCCGGCACAATAGGCGTACTCGCTACAGCATTTGGTTTGATATTCGCGTTATACACAACAAGAATTGCCAAACGTAGTGCCGTAATAGGTAAAATATTCTCTATATTACCTATTGTCACCCCACCATTTGTAg             <  1:308215/251‑1 (MQ=255)                                                                                                                                                                                                                                   cGATCTTTATGTCAATGTTCTATAAAGGGACAGATTTTGTTCCCGGGCAAGTCATAGATATTATAAAGCAGCCTTATGTTGTCAGAATCATCCTAAACTCACTGTCTGTTGCCGGCACAATAGGCGTACTCGCTACAGCATTTGGTTTGATATTCGCGTTATACACAACAAGAATTGCCAAACGTAGTGCCGTAATAGGTAAAATATTCTCTATATTACCTATTGTCACCCCACCATTTGTAGTTGGATTa     <  1:574063/251‑1 (MQ=255)                                                                                                                                                                                                                                     aTCTTTATGTCAATGTTCTATAAAGGGACAGATTTTGTTCCCGGGCAAGTCATAGATATTATAAagc                                                                                                                                                                                           >  1:564561/1‑67 (MQ=255)                                                                                                                                                                                                                                      tCTTTATGTCAATGTTCTATAAAGGGACAGATTTTGTTCCCGGGCAAGTCATAGATATTATAAAGCAGCCTTATGTTGTCAGAATCATCCTAAACTCACTGTCTGTTGCCGGCACAATAGGCGTACTCGCTACAGCATTTGGTTTGATATTCGCGTTATACACAACAAGCATTGCCAAACGTAGTGCCGTAAt                                                            >  1:429108/1‑193 (MQ=255)                                                                                                                                                                                                                                      tCTTTATGTCAATGTTCTATAAAGGGACAGATTTTGTTCCCGGGCAAGTCATAGATATTATAAAGCAGCCTTATGTTGTCAGAATCATCCTAAACTCACTGTCTGTTGCCGGCACAATAGGCGTACTCGCTACAGCATTTGGTTTGATATTCGCGTTATACACAACAAGAATTGCCAAACGTAGTGCCGTAATAGGTAAAATATTCTCTATATTACCTATTGTCACCCCACCATTTGTAGTTGGATTAggg  >  1:782528/1‑251 (MQ=255)                                                                                                                                                                                                                                      tCTTTATGTCAATGTTCTATAAAGGGACAGATTTTGTTCCCGGGCAAGTCATAGATATTATAAAGCAGCCTTATGTTGTCAGAATCATCCTAAACTCACTGTCTGTTGCCGGCACAATAGGCGTACTCGCTACAGCATTTGGTTTGATATTCGCGTTATACACAACAAGAATTGCCAAACGTAGTGCCGTAATAGGTAAAATATTCTCTATATTACCTATTGTCACCCCACCATTTGTAGTTGGATTAggg  >  1:646421/1‑251 (MQ=255)                                                                                                                                                                                                                                      tCTTTATGTCAATGTTCTATAAAGGGACAGATTTTGTTCCCGGGCAAGTCATAGATATTATAAAGCAGCCTTATGTTGTCAGAATCATCCTAAACTCACTGTCTGTTGCCGGCACAATAGGCGTACTCGCTACAGCATTTGGTTTGATATTCGCGTTATACACAACAAGAATTGCCAAACGTAGTGCCGTAATAGGTAAAATATTCTCTATATTACCTATTGTCACCCCACCATTTGTAGTTGGATTAggg  >  1:633032/1‑251 (MQ=255)                                                                                                                                                                                                                                       cTTTATGTCAATGTTCTATAAAGGGACAGATTTTGTTCCCGGGCAAGTCATAGATATTATAAAGCAGCCTTATGTTGTCAGAATCATCCTAAACTCACTGTCTGTTGCCGGCACAATAGGCGTACTCGCTACAGCATTTGGTTTGATATTCGCGTTATACACAACAAGAATTGCCAAACGt                                                                       <  1:577891/181‑1 (MQ=255)                                                                                                                                                                                                                                       cTTTATGTCAATGTTCTATAAAGGGACAGATTTTGTTCCCGGGCAAGTCATAGATATTATAAAGCAGCCTTATGTTGTCAGAATCATCCTAAACTCACTGTCTGTTGCCGGCACAATAGGCGTACTCGCTACAGCATTTGGTTTGATATTCGCGTTATACACAACAAGAATTGCCAAACGTAGTGCCGTAATAGGTAAAATATTCTCTATATTACCTATTGTCAcc                          >  1:80806/1‑226 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                    ATAGAGTTCATTTTATCCTGTATAATTTTTGGATTTGTGATGGTCTCAGCCACAATTGCAAAAATCAGTTTGGGCTATTCTATTTTTGTTCTCACCATAGCACTAATCGGAGTTTCTACGAATGCACTTGCCAAAATGAAAGTCATGCAAAGTGATAAATTCATCATTGGCTCTTTAATTAGCATTGTCCTATTAATATTCTTTTTTATCGTTTATCCAACATTGGCGATCTTTATATCAATGTTCTATAAAGGGACAGATTTTGTTCCCGGGCAAGTCATAGATATTATAAAGCAGCCTTATGTTGTCAGAATCATCCTAAACTCACTGTCTGTTGCCGGCACAATAGGCGTACTCGCTACAGCATTTGGTTTGATATTCGCGTTATACACAACAAGAATTGCCAAACGTAGTGCCGTAATAGGTAAAATATTCTCTATATTACCTATTGTCACCCCACCATTTGTAGTTGGATTAGGG  >  1/1357638‑1358117

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑