Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,353,377	G→A	I64I (ATC→ATT)	unknown ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,353,377	0	G	A	100.0%	191.0 / NA	71	I64I (ATC→ATT)	unknown	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (36/35);  total (36/35)

ATTAAACATTTTATTGTTGCCGTACAAGGGTTTTGCCAATTCCACCGTAACTGAATTATCGCCGCTAAAGACAATGCCATCGGAAGAAATATTATCAAGGGAGAAATCGGAAAAACGCATTTGAATATCG‑‑GCGCTATTCTGCGCGTTTTTAGACCATTTGGCGTTTTGGATTTCCAGCGCCTGTAATTTGACATTCCCAGAAAACAACGCGGATAACGGAAATTTACCGGTCAGTTTTTCAATGCTGATTTGGTTATGTTTAACGTTTTCAAAAGCAAGGTAAGGTTGTGGGAAAAACCCTAGACTAAATACTTGAAATTGGGTTTCATGTTGCGCCAGTTTCGCGGAAACCACCGTTTCCAGTTTCTGAATTTGGACATCAAAAAATGCAAAGATCGCAAGTATGACGACCACAGTAATAATTGCGATCTTTTTCATTTATTTGTCCTCATCAATGCGGCTGGCAACGGCATTTTGTTGGTTCTTGTATTT  >  1/1353131‑1353622                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                      aTTAAACATTTTATTGTTGCCGTACAAGGGTTTTGCCAATTCCACCGTAACTGAATTATCGCCGCTAAAGACAATGCCATCGGAAGAAATATTATCAAGGGAGAAATCGGAAAAACGCATTTGAATATCG‑‑GCGCTATTCTGCGCGTTTTTAGACCATTTGGCGTTTTGGATTTCCAGCGCCTGTAATTTGACATTCCCAGAAAACAACGCGGATAACGGAAATTTACCGGTCAGTTTTTCAATGCTAAttt                                                                                                                                                                                                                                                   >  1:533385/1‑251 (MQ=255)         ttttATTGTTGCCGTACAAGGGTTTTGCCAATTCCACCGTAACTGAATTATCGCCGCTAAAGACAATGCCATCGGAAGAAATATTATCAAGGGAGAAATCGGAAAAACGCATTTGAATATCG‑‑GCGCTATTCTGCGCGTTTTTAGACCATTTGGCGTTTTGGATTTCCAGCGCCTGTAATTTGACATTCCCAGAAAACAACGCGGATAACGGAAATTTACCGGTCAGTTTTTCAATGCTAATTTGGTTATGt                                                                                                                                                                                                                                           >  1:713903/1‑251 (MQ=255)           ttATTGTTGCCGTACAAGGGTTTTGCCAATTCCACCGTAACTGAATTATCGCCGCTAAAGACAATGCCATCGGAAGAAATAGTATCAAGGGAGAAATCGGAAAAACGCATTTGAATATCG‑‑GCGCTATTCTGCGCGTTTTTAGACCATTTGGCGTTTTGGATTTCCAGCGCCTGTAATTTGACATTCCCAGAAAACAACGCGGATAACGGAAATTTACCGGTCAGTTTTTCAATGCTAATTTGGTTATGttt                                                                                                                                                                                                                                         <  1:455116/251‑1 (MQ=255)                 ttgCCGTACAAGGGTTTTGCCAATTCCACCGTAACTGAATTATCGCCGCTAAAGACAATGCCATCGGAAGAAATATTATCAAGGGAGAAATCGGAAAAACGCATTTGAATATCGGCGCGCTATTCTGCGCGTTTTTAGACCATTTGGCGTTTTGGATTTCCAGCGCCTGTAATTTGACATTCCCAGAAAACAACGCGGATAACGGAAATTTACCGGTCAGTTTTTCAATGCTAATTTGGTTATGTTTAACg                                                                                                                                                                                                                                     <  1:468504/251‑1 (MQ=255)                 ttgCCGTACAAGGGTTTTGCCAATTCCACCGTAACTGAATTATCGCCGCTAAAGACAATGCCATCGGAAGAAATATTATCAAGGGAGAAATCGGAAAAACGCATTTGAATATCG‑‑GCGCTATTCTGCGCGTTTTTAGACCATTTGGCGTTTTGGATTTCCAGCGCCTGTAATTTGACATTCCCAGAAAACAACGCGGATAACGGAAATTTACCGGTCAGTTTTTCAATGCTAATTTGGTTATGTTTAACGt                                                                                                                                                                                                                                    <  1:669160/250‑1 (MQ=255)                   gCCGTACAAGGGTTTTGCCAATTCCACCGTAACTGAATTATCGCCGCTAAAGACAATGCCATCGGAAGAAATATTATCAAGGGAGAAATCGGAAAAACGCATTTGAATATCG‑‑GCGCTATTCTGCGCGTTTTTAGACCATTTGGCGTTTTGGATTTCCAGCGCCTGTAATTTGACATTCCCAGAAAACAACGCGGATAACGGAAATTTACCGGTCAGTTTTTCAATGCTAATTTGGTTATGTTTAACGtttt                                                                                                                                                                                                                                 >  1:667938/1‑251 (MQ=255)                      gTACAAGGGTTTTGCCAATTCCACCGTAACTGAATTATCGCCGCTAAAGACAATGCCATCGGAAGAAATATTATCAAGGGAGAAATCGGAAAAACGCATTTGAATATCG‑‑GCGCTATTCTGCGCGTTTTTAGACCATTTGGCGTTTTGGATTTCCAGCGCCTGTAATTTGACATTCCCAGAAAACAACGCGGATAACGGAAATTTACCGGTCAGTTTTTCAATGCTAATTTGGTTATGTTTAACGTTTTCaa                                                                                                                                                                                                                              >  1:891955/1‑251 (MQ=255)                                  tGCCAATTCCACCGTAACTGAATTATCGCCGCTAAAGACAATGCCATCGGAAGAAATATTATCAAGGGAGAAATCGGAAAAACGCATTTGAATATCG‑‑GCGCTATTCTGCGCGTTTTTAGACCATTTGGCGTTTTGGATTTCCAGCGCCTGTAATTTGACATTCCCAGAAAACAACGCGGATAACGGAAATTTACCGGTCAGTTTTTCAATGCTAATTTGGTTATGTTTAACGTTTTCAAAAGCaaggtaag                                                                                                                                                                                                                  >  1:793459/1‑251 (MQ=255)                                    ccAATTCCACCGTAACTGAATTATCGCCGCTAAAGACAATGCCATCGGAAGAAATATTATCAAGGGAGAAATCGGAAAAACGCATTTGAATATCG‑‑GCGCTATTCTGCGCGTCTTTAGACCATTTGGCGTTTTGGATTTCCAGCGCCTGTAATTTGACATTCCCAGAAAACAACGCGGATAACGGAAATTTACCGGTCAGTTTTTCAATGCTAATTTGGTTATGTTTAACGTTTTCAAAAGCaaggtaaggt                                                                                                                                                                                                                <  1:390781/251‑1 (MQ=255)                                     cAATTCCACCGTAACTGAATTATCGCCGCTAAAGACAATGCCATCGGAAGAAATATTATCAAGGGAGAAATCGGAAAAACGCATTTGAATATCG‑‑GCGCTATTCTGCGCGTTTTTAGACCATTTGGCGTTTTGGATTTCCAGCGCCTGTAATTTGACATTCCCAGAAAACAACGCGGATAACGGAAATTTACCGGTCAGTTTTTCAATGCTAATTTGGTTATGTTTAACGTTTTCAAAAGCAAGGTAAGGtt                                                                                                                                                                                                               >  1:411256/1‑251 (MQ=255)                                          ccACCGTAACTGAATTATCGCCGCTAAAGACAATGCCATCGGAAGAAATATTATCAAGGGAGAAATCGGAAAAACGCATTTGAATATCG‑‑GCGCTATTCTGCGCGTTTTTAGACCATTTGGCGTTTTGGATTTCCAGCGCCTGTAATTTGACATTCCCAGAAAACAACGCGGATAACGGAAATTTACCGGTCAGTTTTTCAATGCTAATTTGGTTATGTTTAACGTTTTCAAAAGCAAGGTAAGGTTGTggg                                                                                                                                                                                                          <  1:439994/251‑1 (MQ=255)                                           cACCGTAACTGAATTATCGCCGCTAAAGACAATGCCATCGGAAGAAATATTATCAAGGGAGAAATCGGAAAAACGCATTTGAATATCG‑‑GCGCTATTCTGCGCGTTTTTAGACCATTTGGCGTTTTGGATTTCCAGCGCCTGTAATTTGACATTCCCAGAAAACAACGCGGATAACGGAAATTTACCGGTCAGTTTTTCAATGCTAATTTGGTTATGTTTAACGTTTTCAAAAGCAAGGTAAGGTTGTGGGa                                                                                                                                                                                                         <  1:193144/251‑1 (MQ=255)                                                tAACTGAATTATCGCCGCTAAAGACAATGCCATCGGAAGAAATATTATCAAGGGAGAAATCGGAAAAACGCATTTGAATATCG‑‑GCGCTATTCTGCGCGTTTTTAGACCATTTGGCGTTTTGGATTTCCAGCGCCTGTAATTTGACATTCCCAGAAAACAACGCGGATAACGGAAATTTACCGGTCAGTTTTTCAATGCTAATTTGGTTATGTTTAACGTTTTCAAAAGCaaggtaaggt                                                                                                                                                                                                                <  1:528837/239‑1 (MQ=255)                                                tAACTGAATTATCGCCGCTAAAGACAATGCCATCGGAAGAAATATTATCAAGGGAGAAATCGGAAAAACGCATTTGAATATCG‑‑GCGCTATTCTGCGCGTTTTTAGACCATTTGGCGTTTTGGATTTCCAGCGCCTGTAATTTGACATTCCCAGAAAACAACGCGGATAACGGAAATTTACCGGTCAGTTTTTCAATGCTAATTTGGTTATGTTTAACGTTTTCAAAAGCAAGGTAAGGTTGTGGGAAAAAc                                                                                                                                                                                                    <  1:38833/251‑1 (MQ=255)                                                       aTTATCGCCGCTAAAGACAATGCCATCGGAAGAAATATTATCAAGGGAGAAATCGGAAAATCGCATTTGAATATCG‑‑GCGCTATTCTGCGCGTTTTTAGACCATTTGGCGTTTTGGATTTCCAGCGCCTGTAATTTGACATTCCCAGAAAACAACGCGGATAACGGAAATTTACCGGTCAGTTTTTCAATGCTAATTTGGTTATGTTTAACGTTTTCAAAAGCAAGGTAAGGTTGTGGGAAAAACCCTAGAc                                                                                                                                                                                             <  1:410929/251‑1 (MQ=255)                                                                gcTAAAGACAATGCCATCGGAAGAAATATTATTAAGGGAGAAATCGGAAAAACGCATTTGAATATCG‑‑GCGCTATTCTGCGCGTTTTTAGACCATTTGGCGTTTTGGATTTCCAGCGCCTGTAATTTGACATTCCCAGAAAACAACGCGGATAACGGAAATTTACCGGTCAGTTTTTCAATGCTAATTTGGTTATGTTTAACg                                                                                                                                                                                                                                     <  1:449595/202‑1 (MQ=255)                                                                gcTAAAGACAATGCCATCGGAAGAAATATTATCAAGGGAGAAATCGGAAAAACGCATTTGAATATCG‑‑GCGCTATTCTGCGCGTTTTTAGACCATTTGGCGTTTTGGATTTCCAGCGCCTGTAATTTGACATTCCCAGAAAACAACGCGGATAACGGAAATTTACCGGTCAGTTTTTCAATGCTAAttt                                                                                                                                                                                                                                                   >  1:674129/1‑188 (MQ=255)                                                                gcTAAAGACAATGCCATCGGAAGAAATATTATCAAGGGAGAAATCGGAAAAACGCATTTGAATATCG‑‑GCGCTATTCTGCGCGTTTTTAGACCATTTGGCGTTTTGGATTTCCAGCGCCTGTAATTTGACATTCCCAGAAAACAACGCGGATAACGGAAATTTACCGGTCAGTTTTTCAATGCTAATTTGGTTATGTTTAACGTTTTCAAAAGCAAGGTAAGGTTGTGGGAAAAACCCTAGACTAAATACtt                                                                                                                                                                                    >  1:746459/1‑251 (MQ=255)                                                                gcTAAAGACAATGCCATCGGAAGAAATATTATCAAGGGAGAAATCGGAAAAACGCATTTGAATATCG‑‑GCGCTATTCTGCGCGTTTTTAGACCATTTGGCGTTTTGGATTTCCAGCGCCTGTAATTTGACATTCCCAGAAAACAACGCGGATAACGGAAATTTACCGGTCAGTTTTTCAATGCTAATTTGGTTATGTTTAACGTTTTCAAAAGCAAGGTAAGGTTGTGGGAAAAACCCTAGACTAAATACtt                                                                                                                                                                                    >  1:325191/1‑251 (MQ=255)                                                                gcTAAAGACAATGCCATCGGAAGAAATATTATCAAGGGAGAAATCGGAAAAACGCATTTGAATATCG‑‑GCGCTATTCTGCGCGTTTTTAGACCATTTGGCGTTTTGGATTTCCAGCGCCTGTAATTTGACATTCCCAGAAAACAACGCGGATAACGGAAATTTACCGGTCAGTTTTTCAATGCTAATTTGGTTATGTTTAACGTTTTCAAAAGCAAGGTAAGGTTGTGGGAAAAACCCTAGACTAAATACtt                                                                                                                                                                                    >  1:140759/1‑251 (MQ=255)                                                                gcTAAAGACAATGCCATCGGAAGAAATATTATCAAGGGAGAAATCGGAAAAACGCATTTGAATATCG‑‑GCGCTATTCTGCGCGTTTTTAGACCATTTGGCGTTTTGGATTTCCAGCGCCTGTAATTTGACATTCCCAGAAAACAACGCGGATAACGGAAATTTACCGGTCAGTTTTTCAATGCTAATTTGGTTATGTTTAACGTTTTCAAAAGCAAGGTAAGGTTGTGGGAAAAACCCTAGACTAAATACtt                                                                                                                                                                                    >  1:408635/1‑251 (MQ=255)                                                                gcTAAAGACAATGCCATCGGAAGAAATATTATCAAGGGAGAAATCGGAAAAACGCATTTGAATATCG‑‑GCGCTATTCTGCGCGTTTTTAGACCATTTGGCGTTTTGGATTTCCAGCGCCTGTAATTTGACATTCCCAGAAAACAACGCGGATAACGGAAATTTACCGGTCAGTTTTTCAATGCTAATTTGGTTATGTTTAACGTTTTCAAAAGCAAGGTAAGGTTGTGGGAAAAACCCTAGACTAAATACtt                                                                                                                                                                                    >  1:797619/1‑251 (MQ=255)                                                                 cTAAAGACAATGCCATCGGAAGAAATATTATCAAGGGAGAAATCGGAAAAACGCATTTGAATATCG‑‑GCGCTATTCTGCGCGTTTTTAGACCATTTGGCGTTTTGGATTTCCAGCGCCTGTAATTTGACATTCCCAGAAAACAACGCGGATAACGGAAATTTACCGGTCAGTTTTTCAATGCTAATTTGGTTATGTTTAACGTTTTCAAAAGCAAGGTAAGGTTGTGGGAAAAACCCTAGACTAAATACTTg                                                                                                                                                                                   <  1:102439/251‑1 (MQ=255)                                                                     aGACAATGCCATCGGAAGAAATATTATCAAGGGAGAAATCGGAAAAACGCATTTGAATATCG‑‑GCGCTATTCTGCGCGTTTTTAGACCATTTGGCGTTTTGGATTTCCAGCGCCTGTAATTTGACATTCCCAGAAAACAACGCGGATAACGGAAATTTACCGGTCAGTTTTTCAATGCTAAtt                                                                                                                                                                                                                                                    >  1:104844/1‑182 (MQ=255)                                                                     aGACAATGCCATCGGAAGAAATATTATCAAGGGAGAAATCGGAAAAACGCATTTGAATATCG‑‑GCGCTATTCTGCGCGTTTTTAGACCATTTGGCGTTTTGGATTTCCAGCGCCTGTAATTTGACATTCCCAGAAAACAACGCGGATAACGGAAATTTACCGGTCAGTTTTTCAATGCTAATTTGGTTa                                                                                                                                                                                                                                              <  1:821873/188‑1 (MQ=255)                                                                     aGACAATGCCATCGGAAGAAATATTATCAAGGGAGAAATCGGAAAAACGCATTTGAATATCG‑‑GCGCTATTCTGCGCGTTTTTAGACCATTTGGCGTTTTGGATTTCCAGCGCCTGTAATTTGACATTCCCAGAAAACAACGCGGATAACGGAAATTTACCGGTCAGTTTTTCAATGCTAATTTGGTTATGTTTAACGTTTTCAAAAGCAAGGTAAGGTTGTGGGAAAAACCCTAGACTAAAt                                                                                                                                                                                        >  1:654752/1‑242 (MQ=255)                                                                     aGACAATGCCATCGGAAGAAATATTATCAAGGGAGAAATCGGAAAAACGCATTTGAATATCG‑‑GCGCTATTCTGCGCGTTTTTAGACCATTTGGCGTTTTGGATTTCCAGCGCCTGTAATTTGACATTCCCAGAAAACAACGCGGATAACGGAAATTTACCGGTCAGTTTTTCAATGCTAATTTGGTTATGTTTAACGTTTTCAAAAGCAAGGTAAGGTTGTGGGAAAAACCCTAGACTAAATACTTGAAAt                                                                                                                                                                               >  1:360320/1‑251 (MQ=255)                                                                        cAATGCCATCGGAAGAAATATTATCAAGGGAGAAATCGGAAAAACGCATTTGAATATCG‑‑GCGCTATTCTGCGCGTTTTTAGACCATTTGGCGTTTTGGATTTCCAGCGCCTGTAATTTGACATTCCCAGAAAACAACGCGGATAACGGAAATTTACCGGTCAGTTTTTCAATGCTAATTTGGTTATGTTTAACGTTTTCAAAAGCAAGGTAAGGTTGTGGGAAAAACCCTAGACTAAATACTTGAAATTgg                                                                                                                                                                            >  1:766068/1‑251 (MQ=255)                                                                               aTCGGAAGAAATATTATCAAGGGAGAAATCGGAAAAACGCATTTGAATATCG‑‑GCGCTATTCTGCGCGTTTTTAGACCATTTGGCGTTTTGGATTTCCAGCGCCTGTAATTTGACATTCCCAGAAAACAACGCGGATAACGGAAATTTACCGGTCAGTTTTTCAATGCTAATTTGGTTATGTTTAACGTTTTCAAAAGCAAGGTAAGGTTGTGGGAAAAACCCTAGACTAAATACTTGAAATTGGGTTTCAt                                                                                                                                                                     <  1:107041/251‑1 (MQ=255)                                                                                       aaaTATTATCAAGGGAGAAATCGGAAAAACGCATTTGAATATCG‑‑GCGCTATTCTGCGCGTTTTTAGACCATTTGGCGTTTTGGATTTCCAGCGCCTGTAATTTGACATTCCCAGAAAACAACGCGGATAACGGAAATTTACCGGTCAGTTTTTCAATGCTAATTTGGTTATGTTTAACGTTTTCAAAAGCaaggtaaggt                                                                                                                                                                                                                <  1:647694/200‑1 (MQ=255)                                                                                        aaTATTATCAAGGGAGAAATCGGAAAAACGCATTTGAATATCG‑‑GCGCTATTCTGCGCGTTTTTAGACCATTTGGCGTTTTGGATTTCCAGCGCCTGTAATTTGACATTCCCAGAAAACAACGCGGATAACGGAAATTTACCGGTCAGTTTTTCAATGCTAATTTGGTTATGTTTAACGTTTTCAAAAGCaaggtaaggt                                                                                                                                                                                                                <  1:714298/199‑1 (MQ=255)                                                                                         atatTATCAAGGGAGAAATCGGAAAAACGCATTTGAATATCG‑‑GCGCTATTCTGCGCGTTTTTAGACCATTTGGCGTTTTGGATTTCCAGCGCCTGTAATTTGACATTCCCAGAAAACAACGCGGATAACGGAAATTTACCGGTCAGTTTTTCAATGCTAATTTGGTTATGTTTAACGTTTTCAAAAGCaaggtaaggt                                                                                                                                                                                                                <  1:716618/198‑1 (MQ=255)                                                                                         atatTATCAAGGGAGAAATCGGAAAAACGCATTTGAATATCG‑‑GCGCTATTCTGCGCGTTTTTAGACCATTTGGCGTTTTGGATTTCCAGCGCCTGTAATTTGACATTCCCAGAAAACAACGCGGATAACGGAAATTTACCGGTCAGTTTTTCAATGCTAATTTGGTTATGTTTAACGTTTTCAAAAGCAAGGTAAGGTTGTGGGAAAAACCCTAGACTAAAt                                                                                                                                                                                        <  1:574730/222‑1 (MQ=255)                                                                                         atatTATCAAGGGAGAAATCGGAAAAACGCATTTGAATATCG‑‑GCGCTATTCTGCGCGTTTTTAGACCATTTGGCGTTTTGGATTTCCAGCGCCTGTAATTTGACATTCCCAGAAAACAACGCGGATAACGGAAATTTACCGGTCAGTTTTTCAATGCTAATTTGGTTATGTTTAACGTTTTCAAAAGCAAGGTAAGGTTGTGGGAAAAACCCTAGACTAAATACTTg                                                                                                                                                                                   >  1:892841/1‑227 (MQ=255)                                                                                         atatTATCAAGGGAGAAATCGGAAAAACGCATTTGAATATCG‑‑GCGCTATTCTGCGCGTTTTTAGACCATTTGGCGTTTTGGATTTCCAGCGCCTGTAATTTGACATTCCCAGAAAACAACGCGGATAACGGAAATTTACCGGTCAGTTTTTCAATGCTAATTTGGTTATGTTTAACGTTTTCAAAAGCAAGGTAAGGTTGTGGGAAAAACCCTAGACTAAATACTTGAAATTGGGTTTCATGTTGCGCCAg                                                                                                                                                           >  1:364819/1‑251 (MQ=255)                                                                                          tattatCAAGGGAGAAATCGGAAAAACGCATTTGAATATCG‑‑GCGCTATTCTGCGCGTTTTTAGACCATTTGGCGTTTTGGATTTCCAGCGCCTGTAATTTGACATTACCAGAAAACAACGCGGATAACGGAAATTTACCGGTCAGTTTTTCAATGCTAATTTGGTTATGTTTAACGTTTTCAAAAGCAAGGTAAGGTTGTGGGAAAAACCCTAGACTAAATACTTGAAATTGGGTTTCATGTTGCGCCAGt                                                                                                                                                          >  1:140397/1‑251 (MQ=255)                                                                                                    ggAGAAATCGGAAAAACGCATTTGAATATCG‑‑GCGCTATTCTGCGCGTTTTTAGACCATTTGGCGTTTTGGATTTCCAGCGCCTGTAATTTGACATTCCCAGAAAACAACGCGGATAACGGAAATTTACCGGTCAGTTTTTCAATGCTAATTTGGTTATGTTTAACGTTTTCAAAAGCaaggtaaggt                                                                                                                                                                                                                <  1:861985/187‑1 (MQ=255)                                                                                                           tCGGAAAAACGCATTTGAATATCG‑‑GCGCTATTCTGCGCGTTTTTAGACCATTTGGCGTTTTGGATTTCCAGCGCCTGTAATTTGACATTCCCAGAAAACAACGCGGATAACGGAAATTTACCGGTCAGTTTTTCAATGCTAATTTGGTTATGTTTAACGTTTTCAAAAGCAAGGTAAGGTTGTGGGAAAAACCCTAGACTAAATACTTGAAATTGGGTTTCATGTTGCGCCAGTTTCGCGGAAACCACCGt                                                                                                                                         <  1:807067/251‑1 (MQ=255)                                                                                                                      cATTTGAATATCG‑‑GCGCTATTCTGCGCGTTTTTAGACCATTTGGCGTTTTGGATTTCCAGCGCCTGTAATTTGACATTCCCAGAAAACAACGCGGATAACGGAAATTTACCGGTCAGTTTTTCAATGCTAATTTGGTTATGTTTAACGTTTTCAAAAGCAAGGTAAGGTTGTGGGAAAAACCCTAGACTAAATACTTGAAATTGGGTTTCATGTTGCGCCAGTTTCGCGGAAACCACCGTTTCCAGTTTc                                                                                                                               <  1:402540/250‑1 (MQ=255)                                                                                                                               atCG‑‑GCGCTATTCTGCGCGTTTTTAGACCATTTGGCGTTTTGGATTTCCAGCGCCTGTAATTTGACATTCCCAGAAAACAACGCGGATAACGGAAATTTACCGGTCAGTTTTTCAATGCTAATTTGGTTATGTTTAACGTTTTCAAAAGCAAGGTAAGGTTGTGGGAAAAACCCTAGACTAAATACTTGAAATTGGGTTTCATGTTGCGCCAGTTTCGCGGAAACCACCGTTTCCAGTTTCTGAATTTGGa                                                                                                                     <  1:498271/251‑1 (MQ=255)                                                                                                                                     gcgcTATTCTGCGCGTTTTTAGACCATTTGGCGTTTTGGATTTCCAGCGCCTGTAATTTGACATTCCCAGAAAACAACGCGGATAACGGAAATTTACCGGTCAGTTTTTCAATGCTAATTTGGTTATGTTTAACGTTTTCAAAAGCAAGGTAAGGTTGTGGGAAAAACCCTAGACt                                                                                                                                                                                            <  1:611122/176‑1 (MQ=255)                                                                                                                                     gcgcTATTCTGCGCGTTTTTAGACCATTTGGCGTTTTGGATTTCCAGCGCCTGTAATTTGACATTCCCAGAAAACAACGCGGATAACGGAAATTTACCGGTCAGTTTTTCAATGCTAATTTGGTTATGTTTAACGTTTTCAAAAGCAAGGTAAGGTTGTGGGAAAAACCCTAGACTAAATACTTGAAATTGGGTTTCATGTTGCGCCAGttt                                                                                                                                                        >  1:187014/1‑212 (MQ=255)                                                                                                                                     gcgcTATTCTGCGCGTTTTTAGACCATTTGGCGTTTTGGATTTCCAGCGCCTGTAATTTGACATTCCCAGAAAACAACGCGGATAACGGAAATTTACCGGTCAGTTTTTCAATGCTAATTTGGTTATGTTTAACGTTTTCAAAAGCAAGGTAAGGTTGTGGGAAAAACCCTAGACTAAATACTTGAAATTGGGTTTCATGTTGCGCCAGTTTCGCGGAAACCACCGTTTCCAGTTTCTGAATTTGGACAt                                                                                                                  >  1:289853/1‑250 (MQ=255)                                                                                                                                     gcgcTATTCTGCGCGTTTTTAGACCATTTGGCGTTTTGGATTTCCAGCGCCTGTAATTTGACATTCCCAGAAAACAACGCGGATAACGGAAATTTACCGGTCAGTTTTTCAATGCTAATTTGGTTATGTTTAACGTTTTCAAAAGCAAGGTAAGGTTGTGGGAAAAACCCTAGACTAAATACTTGAAATTGGGTTTCATGTTGCGCCAGTTTCGCGGAAACCACCGTTTCCAGTTTCTGAATTTGGACAt                                                                                                                  >  1:831304/1‑250 (MQ=255)                                                                                                                                          aTTCTGCGCGTTTTTAGACCATTTGGCGTTTTGGATTTCCAGCGCCTGTAATTTGACATTCCCAGAAAACAACGCGGATAACGGAAATTTACCGGTCAGTTTTTCAATGCTAATTTGGTTATGTTTAACGTTTTCAAAAGCAAGGTAAGGTTGTGGGAAAAACCCTAGACTAAATACTTGAAATTGGGTTTCATGTTGCGCCAGTTTCGCGGAAACCACCGTTTCCAGTTTCTGAATTTGGACATCaaaaa                                                                                                            >  1:898553/1‑251 (MQ=255)                                                                                                                                          aTTCTGCGCGTTTTTAGACCATTTGGCGTTTTGGATTTCCAGCGCCTGTAATTTGACATTCCCAGAAAACAACGCGGATAACGGAAATTTACCGGTCAGTTTTTCAATGCTAATTTGGTTATGTTTAACGTTTTCAAAAGCAAGGTAAGGTTGTGGGAAAAACCCTAGACTAAATACTTGAAATTGGGTTTCATGTTGCGCCAGTTTCGCGGAAACCACCGTTTCCAGTTTCTGAATTTGGACATCaaaaa                                                                                                            >  1:550527/1‑251 (MQ=255)                                                                                                                                          aTTCTGCGCGTTTTTAGACCATTTGGCGTTTTGGATTTCCAGCGCCTGTAATTTGACATTCCCAGAAAACAACGCGGATAACGGAAATTTACCGGTCAGTTTTTCAATGCTAATTTGGTTATGTTTAACGTTTTCAAAAGCAAGGTAAGGTTGTGGGAAAAACCCTAGACTAAATACTTGAAATTGGGTTTCATGTTGCGCCAGTTTCGCGGAAACCACCGTTTCCAGTTTCTGAATTTGGACATCaaaaa                                                                                                            <  1:672051/251‑1 (MQ=255)                                                                                                                                          aTTCTGCGCGTTTTTAGACCATTTGGCGTTTTGGATTTCCAGCGCCTGTAATTTGACATTCCCAGAAAACAACGCGGATAACGGAAATTTACCGGTCAGTTTTTCAATGCTAATTTGGTTATGTTTAACGTTTTCAAAAGCAAGGTAAGGTTGTGGGAAAAACCCTAGACTAAATACTTGAAATTGGGTTTCATGTTGCGCCAGTTTCGCGGAAACCACCGTTTCCAGTTTCTGAATTTGGACATCaaaaa                                                                                                            >  1:425581/1‑251 (MQ=255)                                                                                                                                                  cgTTTTTAGACCATTTGGCGTTTTGGATTTCCAGCGCCTGTAATTTGACATTCCCAGAAAACAACGCGGATAACGGAAATTTACCGGTCAGTTTTTCAATGCTAATTTGGTTATGTTTAACGTTTTCAAAAGCAAGGTAAGGTTGTGGGAAAAACCCTAGACTAAATACTTGAAATTGGGTTTCATGTTGCGCCAGTTTCGCGGAAACCACCGTTTCCAGTTTCTGAATTTGGACATCAAAAAATGCAAAg                                                                                                    <  1:815511/251‑1 (MQ=255)                                                                                                                                                    tttttAGACCATTTGGCGTTTTGGATTTCCAGCGCCTGTAATTTGACATTCCCAGAAAACAACGCGGATAACGGAAATTTACCGGTCAGTTTTTCAATGCTAATTTGGTTATGTTTAACGTTTTCAAAAGCAAGGTAAGGTTGTGGGAAAAACCCTAGACTAAATACTTg                                                                                                                                                                                   >  1:55249/1‑170 (MQ=255)                                                                                                                                                    tttttAGACCATTTGGCGTTTTGGATTTCCAGCGCCTGTAATTTGACATTCCCAGAAAACAACGCGGATAACGGAAATTTACCGGTCAGTTTTTCAATGCTAATTTGGTTATGTTTAACGTTTTCAAAAGCAAGGTAAGGTTGTGGGAAAAACCCTAGACTAAATACTTg                                                                                                                                                                                   >  1:115130/1‑170 (MQ=255)                                                                                                                                                    tttttAGACCATTTGGCGTTTTGGATTTCCAGCGCCTGTAATTTGACATTCCCAGAAAACAACGCGGATAACGGAAATTTACCGGTCAGTTTTTCAATGCTAATTTGGTTATGTTTAACGTTTTCAAAAGCAAGGTAAGGTTGTGGGAAAAACCCTAGACTAAATACTTGAAATTGGGTTTCATGTTGCGCCAGTTTCGCGGAAACCACCGTTTCCAGTTTCTGAATTTGGACATCAAAAAATGCAAAGAt                                                                                                  >  1:493506/1‑251 (MQ=255)                                                                                                                                                    tttttAGACCATTTGGCGTTTTGGATTTCCAGCGCCTGTAATTTGACATTCCCAGAAAACAACGCGGATAACGGAAATTTACCGGTCAGTTTTTCAATGCTAATTTGGTTATGTTTAACGTTTTCAAAAGCAAGGTAAGGTTGTGGGAAAAACCCTAGACTAAATACTTGAAATTGGGTTTCATGTTGCGCCAGTTTCGCGGAAACCACCGTTTCCAGTTTCTGAATTTGGACATCAAAAAATGCAAAGAt                                                                                                  >  1:786911/1‑251 (MQ=255)                                                                                                                                                            ccATTTGGCGTTTTGGATTTCCAGCGCCTGTAATTTGACATTCCCAGAAAACAACGCGGATAACGGAAATTTACCGGTCAGTTTTTCAATGCTAATTTGGTTATGttt                                                                                                                                                                                                                                         >  1:112153/1‑108 (MQ=255)                                                                                                                                                            ccATTTGGCGTTTTGGATTTCCAGCGCCTGTAATTTGACATTCCCAGAAAACAACGCGGATAACGGAAATTTACCGGTCAGTTTTTCAATGCTAATTTGGTTATGTTTAACGTTTTCAAAAGCAAGGTAAGGTTGTGGGAAAAACCCTAGGCTAAATTCTTGAAATTGGGTTTCATGTTGCGCCAGTTTCGCGGAAACCACCGTTTCCAGTTTCTGAATTTGGACATCAAAAAATGCAAAGATCGCAAGTa                                                                                          <  1:897565/251‑1 (MQ=255)                                                                                                                                                              aTTTGGCGTTTTGGATTTCCAGCGCCTGTAATTTGACATTCCCAGAAAACAACGCGGATAACGGAAATTTACCGGTCAGTTTTTCAATGCTAATTTGGTTATGTTTAACGTTTTCAAAAGCAAGGTAAGGTTGTGGGAAAAACCCTAGACTAAATACTTGAAATTGGGTTTCATGTTGCGCCAGTTTCGCGGAAACCACCGTTTCCAg                                                                                                                                   >  1:59404/1‑208 (MQ=255)                                                                                                                                                                          ggATTTCCAGCGCCTGTAATTTGACATTCCCAGAAAACAACGCGGATAACGGAAATTTACCGGTCAGTTTTTCAATGCTAATTTGGTTATGTTTAACGTTTTCAAAAGCAAGGTAAGGTTGTGGGAAAAACCCTAGACTAAATACTTGAAATTGGGTTTCATGTTGCGCCAGTTTCGCGGAAACCACCGTTTCCAGTTTCTGAATTTGGACATCAAAAAATGCAAAGATCGCAAGTATGACGACCACAGta                                                                            >  1:90128/1‑251 (MQ=255)                                                                                                                                                                              ttCCAGCGCCTGTAATTTGACATTCCCAGAAAACAACGCGGATAACGGAAATTTACCGGTCAGTTTTTCAATGCTAATTTGGTTGTGTTTAACGTTTTCAAAAGCAAGGTAAGGTTGTGGGAAAAACCCTAGACTAAATACTTGAAATTGGGTTTCATGTTGCGCCAGTTTCGCGGAAACCACCGTTTCCAGTTTCTGAATTTGGACATCAAAAAATGCAAAGATCGCAAGTATGACGACCACAGtaataa                                                                        <  1:49149/251‑1 (MQ=255)                                                                                                                                                                              ttCCAGCGCCTGTAATTTGACATTCCCAGAAAACAACGCGGATAACGGAAATTTACCGGTCAGTTTTTCAATGCTAATTTGGTTATGTTTAACGTTTTCAAAAGCAAGGTAAGGTTGTGGGAAAAACCCTAGACTAAATACTTGAAATTGGGTTTCATGTTGCGCCAGTTTCGCGGAAACCACCGTTTCCAGTTTCTGAATTTGGACATCAAAAAATGCAAAGATCGCAAGTATGACGACCACAGtaataa                                                                        <  1:79144/251‑1 (MQ=255)                                                                                                                                                                                   gcgcCTGTAATTTGACATTCCCAGAAAACAACGCGGATAACGGAAATTTACCGGTCAGTTTTTCAATGCTAATTTGGTTATGTTTAACGTTTTCAAAAGCAAGGTAAGGTTGTGGGAAAAACCCTAGACTAAATACTTGAAATTGGGTTTCATGTTGCGCCAGTTTCGCAGAAACCACCGTTTCCAGTTTCTGaa                                                                                                                           >  1:426301/1‑195 (MQ=255)                                                                                                                                                                                    cgcCTGTAATTTGACATTCCCAGAAAACAACGCGGATAACGGAAATTTACCGGTCAGTTTTTCAATGCTAATTTGGTTATGTTTAACGTTTTCAAAAGCAAGGTAAGGTTGTGGGAAAAACCCTAGACTAAATACTTGAAATTGGGTTTCATGTTGCGCCAGTTTCGCGGAAACCACCGTTTCCAGTTTCTGAATTTGGACATCAAAAAATGCAAAGATCGCAAGTATGACGACCACAGTAATAATTg                                                                     <  1:77988/248‑1 (MQ=255)                                                                                                                                                                                         gTAATTTGACATTCCCAGAAAACAACGCGGATAACGGAAATTTACCGGTCAGTTTTTCAATGCTAATTTGGTTATGTTTAACGTTTTCAAAAGCAAGGTAAGGTTGTGGGAAAAACCCTAGACTAAATACTTGAAATTGGGTTTCATGTTGCGCCAGTTTCGCGGAAACCACCGTTTcc                                                                                                                                     <  1:369259/179‑1 (MQ=255)                                                                                                                                                                                            aTTTGACATTCCCAGAAAACAACGCGGATAACGGAAATTTACCGGTCAGTTTTTCAATGCTAATTTGGTTATGTTTAACGTTTTCAAAAGCAAGGTAAGGTTGTGGGAAAAACCCTAGACTAAATACTTGAAATTGGGTTTCATGTTGCGCCAGTTTCGCGGAAACCACCGTTTCCAGTTTCTGAATTTGGACATCAAAAAATGCAAAGATCGCAAGTATGACGACCACAGTAATAATTGCGATCTTTTTc                                                          <  1:893615/251‑1 (MQ=255)                                                                                                                                                                                                       ccAGAAAACAACGCGGATAACGGAAATTTACCGGTCAGTTTTTCAATGCTAATTTGGTTATGTTTAACGTTTTCAAAAGCAAGGTAAGGTTGTGGGAAAAACCCTAGACTAAATACTTGAAATTGGGTTTCATGTTGCGCCAGTTTCGCGGAAACCACCGTTTCCAGTTTCTGAATTTGGACATCAAAAAATGCAAAGATCGCAAGTATGACGACCACAGTAATAATTGCGATCTTTTTCATTTATTTGt                                                >  1:424694/1‑250 (MQ=255)                                                                                                                                                                                                           aaaaCAACGCGGATAACGGAAATTTACCGGTCAGTTTTTCAATGCTAATTTGGTTATGTTTAACGTTTTCAAAAGCAAGGTAAGGTTGTGGGAAAAACCCTAGACTAAATACTTGAAATTGGGTTTCATGTTGCGCCAGTTTCGCGGAAACCACCGTTTCCAGTTTCTGAATTTGGACATCAAAAAATGCAAAGATCGCAAGTATGACGACCACAGTAATAATTGCGATCTTTTTCATTTATTTGTcc                                              <  1:430819/248‑1 (MQ=255)                                                                                                                                                                                                            aaaCAACGCGGATAACGGAAATTTACCGGTCAGTTTTTCAATGCTAATTTGGTTATGTTTAACGTTTTCAAAAGCAAGGTAAGGTTGTGGGAAAAACCCTAGACTAAATACTTGAAATTGGGTTTCATGTTGCGCCAGTTTCGCGGAAACCACCGTTTCCAGTTTCTGAATTTGGACATCAAAAAATGCAAAGATCGCAAGTATGACGACCACAGTAATAATTGCGATCTTTTTCATTTATTTGTCCtcat                                          >  1:747937/1‑251 (MQ=255)                                                                                                                                                                                                                     ggATAACGGAAATTTACCGGTCAGTTTTTCAATGCTAATTTGGTTATGTTTAACGTTTTCAAAAGCAAGGTAAGGTTGTGGGAAAAACCCTAGACTAAATACTTGAAATTGGGTTTCATGTTGCGCCAGTTTCGCGGAAACCACCGTTTCCAGTTTCTGAATTTGGACATCAAAAAATGCAAAGATCGCAAGTATGACGACCACAGTAATAATTGCGATCTTTTTCATTTATTTGTCCtcatc                                         <  1:643067/243‑1 (MQ=255)                                                                                                                                                                                                                             gAAATTTACCGGTCAGTTTTTCAATGCTAATTTGGTTATGTTTAACGTTTTCAAAAGCaaggt                                                                                                                                                                                                                     >  1:92100/1‑63 (MQ=255)                                                                                                                                                                                                                             gAAATTTACCGGTCAGTTTTTCAATGCTAATTTGGTTATGTTTAACGTTTTCAAAAGCAAGGTAAGGTTGTGGGAAAAACCCTAGACTAAATACTTGAAATTGGGTTTCATGTTGCGCCAGTTTCGCGGAAACCACCGTTTCCAGTTTCTGAATTTGGACATCAAAAAATGCAAAGATCGCAAGTATGACGACCACAGTAATAATTGCGATCTTTTTCat                                                        <  1:53966/220‑1 (MQ=255)                                                                                                                                                                                                                                aTTTACCGGTCAGTTTTTCAATGCTAATTTGGTTATGTTTAACGTTTTCAAAAGCAAGGTAAGGTTGTGGGAAAAACCCTAGACTAAATACTTGAAATTGGGTTTCATGTTGCGCCAGTTTCGCGGAAACCACCGTTTCCAGTTTCTGAATTTGGACATCAAAAAATGCAAAGATCGCAAGTATGACGACCACAGtaataa                                                                        <  1:564316/201‑1 (MQ=255)                                                                                                                                                                                                                                                    aTGCTAATTTGGTTATGTTTAACGTTTTCAAAAGCAAGGTAAGGTTGTGGGAAAAACCCTAGACTAAATACTTGAAATTGGGTTTCATGTTGCGCCAGTTTCGCGGAAACCACCGTTTCCAGTTTCTGAATTTGGACATCAAAAAATGCAAAGATCGCAAGTATGACGACCACAGTAATAATTGCGATCTTTTTCATTTATTTGTCCTCATCAATGCGGCTGGCAACGGCATTTTGTTGGTTCTTGTAttt  <  1:39613/251‑1 (MQ=255)                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                      ATTAAACATTTTATTGTTGCCGTACAAGGGTTTTGCCAATTCCACCGTAACTGAATTATCGCCGCTAAAGACAATGCCATCGGAAGAAATATTATCAAGGGAGAAATCGGAAAAACGCATTTGAATATCG‑‑GCGCTATTCTGCGCGTTTTTAGACCATTTGGCGTTTTGGATTTCCAGCGCCTGTAATTTGACATTCCCAGAAAACAACGCGGATAACGGAAATTTACCGGTCAGTTTTTCAATGCTGATTTGGTTATGTTTAACGTTTTCAAAAGCAAGGTAAGGTTGTGGGAAAAACCCTAGACTAAATACTTGAAATTGGGTTTCATGTTGCGCCAGTTTCGCGGAAACCACCGTTTCCAGTTTCTGAATTTGGACATCAAAAAATGCAAAGATCGCAAGTATGACGACCACAGTAATAATTGCGATCTTTTTCATTTATTTGTCCTCATCAATGCGGCTGGCAACGGCATTTTGTTGGTTCTTGTATTT  >  1/1353131‑1353622

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑