Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,347,559	T→C	V314A (GTG→GCG)	unknown →	Adenylosuccinate synthetase (EC 6.3.4.4)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,347,559	0	T	C	100.0%	188.9 / NA	68	V314A (GTG→GCG)	unknown	Adenylosuccinate synthetase (EC 6.3.4.4)
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (41/27);  total (41/27)

GCACCTATCCGTATGTAACCAGCTCCAACACCACCGCCGGCGGCGTAGCGACAGGCTCCGGTTTCGGTCCGCGCAATCTGGATTATGTGTTGGGAATCATCAAAGCCTACTGCACCCGCGTGGGCGGCGGTCCGTTCACTACGGAATTATTTGATGAAACAGGTGCTGAAATCGCCCGTAAAGGTAACGAATTCGGCGCGGTTACCGGTCGTCCGCGTCGTTGCGGTTGGTTTGACGCGGTGGCAATTCGTCGCGCTATTCAACTGAACTCCATTTCCGGTTTCTGCATGACCAAATTAGACGTGCTGGACGGTTTCGATGAAGTGAAAATCTGCGTCGGCTACAAAATGCCGAACGGTGACATCGTGGAATACGCCCCGCTCGCCGCGAAAGATTGGGAAGGCGTTGAACCGATTTACGAAACCTTGCCGGGCTGGAAAGAAAACACCTTCGGCATCACCGATGTTAACCAATTGCCGCAAACC  >  1/1347319‑1347803                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                     gCACCTATCCGTATGTAACCAGCTCCAACACCACCGCCGGCGGCGTAGCGACAGGCTCCGGTTTCGGTCCGCGCAATCTGGATTATGTGTTGGGAATCATCAAAGCCTACTGCACCCGCGTGGGCGGCGGTCCGTTCACTACGGAATTATTTGATGAAACAGGTGCTGAAATCGCCCGTAAAGGTAACGAATTCGGCGCGGTTACCGGTCGTCCGCGTCGTTGCGGTTGGTTTGACGCGGCGGCAATtcgt                                                                                                                                                                                                                                            >  1:23968/1‑251 (MQ=255)   aCCTATCCGTATGTAACCAGCTCCAACACCACCGCCGGCGGCGTAGCGACAGGCTCCGGTTTCGGTCCGCGCAATCTGGATTATGTGTTGGGAATCATCAAAGCCTACTGCACCCGCGTGGGCGGCGGTCCGTTCACTACGGAATTATTTGATGAAACAGGTGCTGAAATCGCCCGTAAAGGTAACGAATTCGGCGCGGTTACCGGTCGTCCGCGTCGTTGCGGTTGGTTTGACGCGGCGGCAATtcgtcg                                                                                                                                                                                                                                          >  1:650481/1‑251 (MQ=255)       aTCCGTATGTAACCAGCTCCAACACCACCGCCGGCGGCGTAGCGACAGGCTCCGGTTTCGGTCCGCGCAATCTGGATTATGTGTTGGGAATCATCAAAGCCTACTGCACCCGCGTGGGCGGCGGTCCGTTCACTACGGAATTATTTGATGAAACAGGTGCTGAAATCGCCCGTAAAGGTAACGAATTCGGCGCGGTTACCGGTCGTCCGCGTCGTTGCGGTTGGTTTGACGCGGCGGCAATTCGTcgcg                                                                                                                                                                                                                                        >  1:41423/1‑249 (MQ=255)        tCCGTATGTAACCAGCTCCAACACCACCGCCGGCGGCGTAGCGACAGGCTCCGGTTTCGGTCCGCGCAATCTGGATTATGTGTTGGGAATCATCAAAGCCTACTGCACCCGCGTGGGCGGCGGTCCGTTCACTACGGAATTATTTGATGAAACAGGTGCTGAAATCGCCCGTAAAGGTAACGAATTCGGCGCGGTTACCGGTCGTCCGCGTCGTTGCGGTTGGTTTGACGCGGCGGCAATTCGTCGCGCTa                                                                                                                                                                                                                                     >  1:491567/1‑251 (MQ=255)           gTATGTAACCAGCTCCAACACCACCGCCGGCGGCGTAGCGACAGGCTCCGGTTTCGGTCCGCGCAATCTGGATTATGTGTTGGGAATCATCAAAGCCTACTGCACCCGCGTGGGCGGCGGTCCGTTCACTACGGAATTATTTGATGAAACAGGTGCTGAAATCGCCCGTAAAGGTAACGAATTCGGCGCGGTTACCGGTCGTCCGCGTCGTTGCGGTTGGTTTGACGCGGCGGCAATTCGTCGCGCTAtt                                                                                                                                                                                                                                   >  1:22861/1‑250 (MQ=255)           gTATGTAACCAGCTCCAACACCACCGCCGGCGGCGTAGCGACAGGCTCCGGTTTCGGTCCGCGCAATCTGGATTATGTGTTGGGAATCATCAAAGCCTACTGCACCCGCGTGGGCGGCGGTCCGTTCACTACGGAATTATTTGATGAAACAGGTGCTGAAATCGCCCGTAAAGGTAACGAATTCGGCGCGGTTACCGGTCGTCCGCGTCGTTGCGGTTGGTTTGACGCGGCGGCAATTCGTCGCGCTAtt                                                                                                                                                                                                                                   >  1:356962/1‑250 (MQ=255)            tATGTAACCAGCTCCAACACCACCGCCGGCGGCGTAGCGACAGGCTCCGGTTTCGGTCCGCGCAATCTGGATTATGTGTTGGGAATCATCAAAGCCTACTGCACCCGCGTGGGCGGCGGTCCGTTCACTACGGAATTATTTGATGAAACAGGTGCTGAAATCGCCCGTAAAGGTAACGAATTCGGCGCGGTTACCGGTCGTCCGCGTCGTTGCGGTTGGTTTGACGCGGCGGCAATTCGTCGCGCTATTCa                                                                                                                                                                                                                                 >  1:231256/1‑251 (MQ=255)              tGTAACCAGCTCCAACACCACCGCCGGCGGCGTAGCGACAGGCTCCGGTTTCGGTCCGCGCAATCTGGATTATGTGTTGGGAATCATCAAAGCCTACTGCACCCGCGTGGGCGGCGGTCCGTTCACTACGGAATTATTTGATGAAACAGGTGCTGAAATCGCCCGTAAAGGTAACGAATTCGGCGCGGTTACCGGTCGTCCGCGTCGTTGCGGTTGGTTTGACGCGGCGGCAATTCGTCGCGCTATTCAAc                                                                                                                                                                                                                               <  1:555712/251‑1 (MQ=255)                      gCTCCAACACCACCGCCGGCGGCGTAGCGACAGGCTCCGGTTTCGGTCCGCGCAATCTGGATTATGTGTTGGGAATCATCAAAGCCTACTGCACCCGCGTGGGCGGCGGTCCGTTCACTACGGAATTATTTGATGAAACAGGTGCTGAAATCGCCCGTAAAGGTAACGAATTCGGCGCGGTTACCGGTCGTCCGCGTCGTTGCGGTTGGTTTGACGCGGCGGCAATTCGTCGCGCTATTCAACTGAACTc                                                                                                                                                                                                                        >  1:566447/1‑250 (MQ=255)                      gCTCCAACACCACCGCCGGCGGCGTAGCGACAGGCTCCGGTTTCGGTCCGCGCAATCTGGATTATGTGTTGGGAATCATCAAAGCCTACTGCACCCGCGTGGGCGGCGGTCCGTTCACTACGGAATTATTTGATGAAACAGGTGCTGAAATCGCCCGTAAAGGTAACGAATTCGGCGCGGTTACCGGTCGTCCGCGTCGTTGCGGTTGGTTTGACGCGGCGGCAATTCGTCGCGCTATTCAACTGAACTc                                                                                                                                                                                                                        >  1:780622/1‑250 (MQ=255)                                  ccgccgGCGGCGTAGCGACAGGCTCCGGTTTCGGTCCGCGCAATCTGGATTATGTGTTGGGAATCATCAAAGCCTACTGCACCCGCGTGGGCGGCGGTCCGTTCACTACGGAATTATTTGATGAAACAGGTGCTGAAATCGCCCGTAAAGGTAACGAATTCGGCGCGGTTACCGGTCGTCCGCGTCGTTGCGGTTGGTTTGACGCGGCGGCAATTCGTCGCGCTATTCAACTGAACTCCATTTCCGGttt                                                                                                                                                                                                            >  1:632078/1‑250 (MQ=255)                                                       gCTCCGGTTTCGGTCCGCGCAATCTGGATTATGTGTTGGGAATCATCAAAGCCTACTGCACCCGCGTGGGCGGCGGTCCGTTCACTACGGAATTATTTGATGAAACAGGTGCTGAAATCGCCCGTAAAGGTAACGAATTCGGCGCGGTTACCGGTCGTCCGCGTCGTTGCGGTTGGTTTGACGCGGCGGCAATTCGTCGCGCTATTCAACTGAACTCCATTTCCGGTTTCTGCATGACCAAATTAGACg                                                                                                                                                                                        <  1:877787/249‑1 (MQ=255)                                                       gCTCCGGTTTCGGTCCGCGCAATCTGGATTATGTGTTGGGAATCATCAAAGCCTACTGCACCCGCGTGGGCGGCGGTCCGTTCACTACGGAATTATTTGATGAAACAGGTGCTGAAATCGCCCGTAAAGGTAACGAATTCGGCGCGGTTACCGGTCGTCCGCGTCGTTGCGGTTGGTTTGACGCGGCGGCAATTCGTCGCGCTATTCAACTGAACTCCATTTCCGGTTTCTGCATGACCAAATTAGACGTg                                                                                                                                                                                      >  1:396834/1‑251 (MQ=255)                                                        cTCCGGTTTCGGTCCGCGCAATCTGGATTATGTGTTGGGAATCATCAAAGCCTACTGCACCCGCGTGGGCGGCGGTCCGTTCACTACGGAATTATTTGATGAAACAGGTGCTGAAATCGCCCGTAAAGGTAACGAATTCGGCGCGGTTACCGGTCGTCCGCGTCGTTGCGGTTGGTTTGACGCGGCGGCAATTCGTCGCGCTATTCAAc                                                                                                                                                                                                                               >  1:223046/1‑209 (MQ=255)                                                             gTTTCGGTCCGCGCAATCTGGATTATGTGTTGGGAATCATCAAAGCCTACTGCACCCGCGTGGGCGGCGGTCCGTTCACTACGGAATTATTTGATGAAACAGGTGCTGAAATCGCCCGTAAAGGTAACGAATTCGGCGCGGTTACCGGTCGTCCGCGTCGTTGCGGTTGGTTTGACGCGGCGGCAATTCGTCGCGCTATTCAACTGAACTCCATTTCCGGTTTCTGCATGACCAAATTAGACGTGCTGGa                                                                                                                                                                                 >  1:368205/1‑250 (MQ=255)                                                             gTTTCGGTCCGCGCAATCTGGATTATGTGTTGGGAATCATCAAAGCCTACTGCACCCGCGTGGGCGGCGGTCCGTTCACTACGGAATTATTTGATGAAACAGGTGCTGAAATCGCCCGTAAAGGTAACGAATTCGGCGCGGTTACCGGTCGTCCGCGTCGTTGCGGTTGGTTTGACGCGGCGGCAATTCGTCGCGCTATTCAACTGAACTCCATTTCCGGTTTCTGCATGACCAAATTAGACGTGCTGGa                                                                                                                                                                                 >  1:30021/1‑250 (MQ=255)                                                                        cgcAATCTGGATTATGTGTTGGGAATCATCAAAGCCTACTGCACCCGCGTGGGCGGCGGTCCGTTCACTACGGAATTATTTGATGAAACAGGTGCTGAAATCGCCCGTAAAGGTAACGAATTCGGCGCGGTTACCGGTCGTCCGCGTCGTTGCGGTTGGTTTGACGCGGCGGCAATTCGTCGCGCTATTCAACTGAACTCCATTTCCGGTTTCTGCATGACCAAATTAGACGTGCTGGACGGTTTCGATgc                                                                                                                                                                     <  1:481487/251‑2 (MQ=255)                                                                           aaTCTGGATTATGTGTTGGGAATCATCAAAGCCTACTGCACCCGCGTGGGCGGCGGTCCGTTCACTACGGAATTATTTGATGAAACAGGTGCTGAAATCGCCCGTAAAGGTAACGAATTCGGCGCGGTTACCGGTCGTCCGCGTCGTTGCGGTTGGTTTGACGCGGCGGCAATTCGTCGCGCTATTCAACTGAACTCCATTTCCGGTTTCTGCATGACCAAATTAGACGTGCTGGACGGTTTCGATGAAGt                                                                                                                                                                  >  1:649898/1‑251 (MQ=255)                                                                            aTCTGGATTATGTGTTGGGAATCATCAAAGCCTACTGCACCCGCGTGGGCGGCGGTCCGTTCACTACGGAATTATTTGATGAAACAGGTGCTGAAATCGCCCGTAAAGGTAACGAATTCGGCGCGGTTACCGGTCGTCCGCGTCGTTGCGGTTGGTTTGACGCGGCGGCAATTCGTCGCGCTATTCAAc                                                                                                                                                                                                                               >  1:296682/1‑189 (MQ=255)                                                                                ggATTATGTGTTGGGAATCATCAAAGCCTACTGCACCCGCGTGGGCGGCGGTCCGTTCACTACGGAATTATTTGATGAAACAGGTGCTGAAATCGCCCGTAAAGGTAACGAATTCGGCGCGGTTACCGGTCGTCCGCGTCGTTGCGGTTGGTTTGACGCGGCGGCAATTCGTCGCGCTATTCAACTGAACTCCATTTCCGGTTTCTGCATGACCAAATTAg                                                                                                                                                                                           >  1:100738/1‑221 (MQ=255)                                                                                ggATTATGTGTTGGGAATCATCAAAGCCTACTGCACCCGCGTGGGCGGCGGTCCGTTCACTACGGAATTATTTGATGAAACAGGTGCTGAAATCGCCCGTAAAGGTAACGAATTCGGCGCGGTTACCGGTCGTCCGCGTCGTTGCGGTTGGTTTGACGCGGCGGCAATTCGTCGCGCTATTCAACTGAACTCCATTTCCGGTTTCTGCATGACCAAATTAGACGTGCTGGACGGTTTCGATGAAGTGaaaa                                                                                                                                                             >  1:499722/1‑251 (MQ=255)                                                                                ggATTATGTGTTGGGAATCATCAAAGCCTACTGCACCCGCGTGGGCGGCGGTCCGTTCACTACGGAATTATTTGATGAAACAGGTGCTGAAATCGCCCGTAAAGGTAACGAATTCGGCGCGGTTACCGGTCGTCCGCGTCGTTGCGGTTGGTTTGACGCGGCGGCAATTCGTCGCGCTATTCAACTGAACTCCATTTCCGGTTTCTGCATGACCAAATTAGACGTGCTGGACGGTTTCGATGAAGTGaaaa                                                                                                                                                             >  1:9571/1‑251 (MQ=255)                                                                                ggATTATGTGTTGGGAATCATCAAAGCCTACTGCACCCGCGTGGGCGGCGGTCCGTTCACTACGGAATTATTTGATGAAACAGGTGCTGAAATCGCCCGTAAAGGTAACGAATTCGGCGCGGTTACCGGTCGTCCGCGTCGTTGCGGTTGGTTTGACGCGGCGGCAATTCGTCGCGCTATTCAACTGAACTCCATTTCCGGTTTCTGCATGACCAAATTAGACGTGCTGGACGGTTTCGATGAAGTGaaaa                                                                                                                                                             >  1:240061/1‑251 (MQ=255)                                                                                 gATTATGTGTTGGGAATCATCAAAGCCTACTGCACCCGCGTGGGCGGCGGTCCGTTCACTACGGAATTATTTGATGAAACAGGTGCTGAAATCGCCCGTAAAGGTAACGAATTCGGCGCGGTTACCGGTCGTCCGCGTCGTTGCGGTTGGTTTGACGCGGCGGCAATTCGTCGCGCTATTCAACTGAACTCCATTTCCGGTTTCTGCATGACCAAAtt                                                                                                                                                                                             >  1:194265/1‑218 (MQ=255)                                                                                       gtgtTGGGAATCATCAAAGCCTACTGCACCCGCGTGGGCGGCGGTCCGTTCACTACGGAATTATTTGATGAAACAGGTGCTGAAATCGCCCGTAAAGGTAACGAATTCGGCGCGGTTACCGGTCGTCCGCGTCGTTGCGGTTGGTTTGACGCGGCGGCAATTCGTCGCGCTATTCAACTGAACTCCATTTCCGGTTTc                                                                                                                                                                                                           <  1:194951/198‑1 (MQ=255)                                                                                       gtgtTGGGAATCATCAAAGCCTACTGCACCCGCGTGGGCGGCGGTCCGTTCACTACGGAATTATTTGATGAAACAGGTGCTGAAATCGCCCGTAAAGGTAACGAATTCGGCGCGGTTACCGGTCGTCCGCGTCGTTGCGGTTGGTTTGACGCGGCGGCAATTCGTCGCGCTATTCAACTGAACTCCATTTCCGGTTTCTGCATGACCAAATTAGACGTGCTGGACgg                                                                                                                                                                              >  1:557435/1‑227 (MQ=255)                                                                                                 tcatcaAAGCCTACTGCACCCGCGTGGGCGGCGGTCCGTTCACTACGGAATTATTTGATGAAACAGGTGCTGAAATCGCCCGTAAAGGTAACGAATTCGGCGCGGTTACCGGTCGTCCGCGTCGTTGCGGTTGGTTTGACGCGGCGGCAATTCGTCGCGCTATTCAACTGAACTCCATTTCCGGTTTCTGCATGACCAAATTAGACGTGCTGGACGGTTTCGATGAAGTGAAAATCTGCGTCGGCTACaaa                                                                                                                                            <  1:576103/251‑1 (MQ=255)                                                                                                  catcaAAGCCTACTGCACCCGCGTGGGCGGCGGTCCGTTCACTACGGAATTATTTGATGAAACAGGTGCTGAAATCGCCCGTAAAGGTAACGAATTCGGCGCGGTTACCGGTCGTCCGCGTCGTTGCGGTTGGTTTGACGCGGCGGCAATTCGTCGCGCTATTCAACTGAACTCCATTTCCGGTTTCTGCATGACCAAATTAGACGTGCTGGAc                                                                                                                                                                                <  1:408990/214‑1 (MQ=255)                                                                                                  catcaAAGCCTACTGCACCCGCGTGGGCGGCGGTCCGTTCACTACGGAATTATTTGATGAAACAGGTGCTGAAATCGCCCGTAAAGGTAACGAATTCGGCGCGGTTACCGGTCGTCCGCGTCGTTGCGGTTGGTTTGACGCGGCGGCAATTCGTCGCGCTATTCAACTGAACTCCATTTCCGGTTTCTGCATGACCAAATTAGACGTGCTGGACGGTTTCGATGAAGTGAAAATCTGCGTCGGCTACaaaa                                                                                                                                           >  1:574781/1‑251 (MQ=255)                                                                                                         gCCTACTGCACCCGCGTGGGCGGCGGTCCGTTCACTACGGAATTATTTGATGAAACAGGTGCTGAAATCGCCCGTAAAGGTAACGAATTCGGCGCGGTTACCGGTCGTCCGCGTCGTTGCGGTTGGTTTGACGCGGCGGCAATTCGTCGCGCTATTCAACTg                                                                                                                                                                                                                             <  1:563535/162‑1 (MQ=255)                                                                                                               tGCACCCGCGTGGGCGGCGGTCCGTTCACTACGGAATTATTTGATGAAACAGGTGCTGAAATCGCCCGTAAAGGTAACGAATTCGGCGCGGTTACCGGTCGTCCGCGTCGTTGCGGTTGGTTTGACGCGGCGGCAATTCGTCGCGCTATTCAACTGAACTCCATTTCCGGTTTCTGCATGACCAAATTAGACGTGCTGGACGGTTTCGATGAAGTGAAAATCTGCGTCGGCTACAAAATGCCGAACGGTGa                                                                                                                              <  1:472507/251‑1 (MQ=255)                                                                                                                     cgcgTGGGCGGCGGTCCGTTCACTACGGAATTATTTGATGAAACAGGTGCTGAAATCGCCCGTAAAGGTAACGAATTCGGCGCGGTTACCGGTCGTCCGCGTCGTTGCGGTTGGTTTGACGCGGCGGCAATTCGTCGCGCTATTCAAc                                                                                                                                                                                                                               >  1:864322/1‑148 (MQ=255)                                                                                                                     cgcgTGGGCGGCGGTCCGTTCACTACGGAATTATTTGATGAAACAGGTGCTGAAATCGCCCGTAAAGGTAACGAATTCGGCGCGGTTACCGGTCGTCCGCGTCGTTGCGGTTGGTTTGACGCGGCGGCAATTCGTCGCGCTATTCAACTGAACTCCATTTCCGGTTTCTGCATGACCAAAtt                                                                                                                                                                                             <  1:631115/182‑1 (MQ=255)                                                                                                                       cgTGGGCGGCGGTCCGTTCACTACGGAATTATTTGATGAAACAGGTGCTGAAATCGCCCGTAAAGGTAACGAATTCGGCGCGGTTACCGGTCGTCCGCGTCGTTGCGGTTGGTTTGACGCGGCGGCAATTCGTCGCGCTATTCAACTGAACTCCATTTCCGGTTTCTGCATGACCAAATTAGACGTGCTGGACGGTTTCGATGAAGTGAAAATCTGCGTCGGCTACAAAATGCCGAACGGTGACATCGTgg                                                                                                                      <  1:237259/251‑1 (MQ=255)                                                                                                                               gcggTCCGTTCACTACGGAATTATTTGATGAAACAGGTGCTGAAATCGCCCGTAAAGGTAACGAATTCGGCGCGGTTACCGGTCGTCCGCGTCGTTGCGGTTGGTTTGACGCGGCGGCAATTCGTCGCGCTATTCAACTGAACTCCATTTCCGGTTTCTGCATGACCAAATTAGACGTGCTGGACGGTTTCGATGAAGTGAAAATCTGCGTCGGCTACAAAATGCCGAACGGTGACATCGTGGAATACGc                                                                                                               >  1:872289/1‑250 (MQ=255)                                                                                                                                    ccGTTCACTACGGAATTATTTGATGAAACAGGTGCTGAAATCGCCCGTAAAGGTAACGAATTCGGCGCGGTTACCGGTCGTCCGCGTCGTTGCGGTTGGTTTGACGCGGCGGCAATTCGTCGCGCTATTCAACTGAACTCCATTTCCGGTTTCTGCATGACCAAATTAGACGTGCTGGACGGTTTCGATGAAGTGAAAATCTGCGTCGGCTACAAAATGCCGAACGGTGACATCGTGGAATACGcc                                                                                                              >  1:142396/1‑246 (MQ=255)                                                                                                                                      gTTCACTACGGAATTATTTGATGAAACAGGTGCTGAAATCGCCCGTAAAGGTAACGAATTCGGCGCGGTTACCGGTCGTCCGCGTCGTTGCGGTTGGTTTGACGCGGCGGCAATTCGTCGCGCTATTCAACTGAACTCCATTTCCGGTTTCTGCATGACCAAATTAGACGTGCTGGACGGTTTCGATGAAGTGAAAATCTGCGTCGGCTACAAAATGCCGAACGGTGACATCGTGGAATACGCCCCGCTcg                                                                                                       >  1:149813/1‑251 (MQ=255)                                                                                                                                      gTTCACTACGGAATTATTTGATGAAACAGGTGCTGAAATCGCCCGTAAAGGTAACGAATTCGGCGCGGTTACCGGTCGTCCGCGTCGTTGCGGTTGGTTTGACGCGGCGGCAATTCGTCGCGCTATTCAACTGAACTCCATTTCCGGTTTCTGCATGACCAAATTAGACGTGCTGGACGGTTTCGATGAAGTGAAAATCTGCGTCGGCTACAAAATGCCGAACGGTGACATCGTGGAATACGCCCCGCTcg                                                                                                       >  1:814763/1‑251 (MQ=255)                                                                                                                                      gTTCACTACGGAATTATTTGATGAAACAGGTGCTGAAATCGCCCGTAAAGGTAACGAATTCGGCGCGGTTACCGGTCGTCCGCGTCGTTGCGGTTGGTTTGACGCGGCGGCAATTCGTCGCGCTATTCAACTGAACTCCATTCCCGGTTTCTGCATGACCAAATTAGACGTGCTGGACGGTTTCGATGAAGTGAAAATCTGCGTCGGCTACAAAATGCCGAACGGTGACATCGTGGAATACGcc                                                                                                              >  1:176559/1‑244 (MQ=255)                                                                                                                                           cTACGGAATTATTTGATGAAACAGGTGCTGAAATCGCCCGTAAAGGTAACGAATTCGGCGCGGTTACCGGTCGTCCGCGTCGTTGCGGTTGGTTTGACGCGGCGGCAATTCGTCGCGCTATTCAACTGAACTCCATTTCCGGTTTCTGCATGACCAAATTAGACGTGCTGGACGGTTTCGATGAAGTGAAAATCTGCGTCGGCTACAAAATGCCGAACGGTGACATCGTGGAATACGCCCCGCTCGCcgcg                                                                                                  >  1:493823/1‑251 (MQ=255)                                                                                                                                                gAATTATTTGATGAAACAGGTGCTGAAATCGCCCGTAAAGGTAACGAATTCGGCGCGGTTACCGGTCGTCCGCGTCGTTGCGGTTGGTTTGACGCGGCGGCAATTCGTCGCGCTATTCAACTGAACTCCATTTCCGGTTTCTGCATGACCAAATTAGACGTGCTGGACGGTTTCGATGAAGTGAAAATCTGCGTCGGCTACAAAATGCCGAACGGTGACATCGTGGAATACGCCCCGCTCGCCGCGAAAGa                                                                                             >  1:628136/1‑251 (MQ=255)                                                                                                                                                gAATTATTTGATGAAACAGGTGCTGAAATCGCCCGTAAAGGTAACGAATTCGGCGCGGTTACCGGTCGTCCGCGTCGTTGCGGTTGGTTTGACGCGGCGGCAATTCGTCGCGCTATTCAACTGAACTCCATTTCCGGTTTCTGCATGACCAAATTAGACGTGCTGGACGATTTCGATGAAGTGAAAATCTGCGTCGGCTACAAAATGCCGAACGGTGACATCGTGGAATACGCCCCGCTCGCCGCGAAAg                                                                                              >  1:448794/1‑250 (MQ=255)                                                                                                                                                       ttGATGAAACAGGTGCTGAAATCGCCCGTAAAGGTAACGAATTCGGCGCGGTTACCGGTCGTCCGCGTCGTTGCGGTTGGTTTGACGCGGCGGCAATTCGTCGCGCTATTCAACTGAACTCCATTTCCGGTTTCTGCATGACCAAATTAGACGTGCTGGACGGTTTCGATGAAGTGAAAATCTGCGTCGGCTACAAAATGCCGAACGGTGACATCGTGGAATACGCCCCGCTCGCCGCGAAAGATTGGGaa                                                                                      <  1:725357/251‑1 (MQ=255)                                                                                                                                                        tgatgaAACAGGTGCTGAAATCGCCCGTAAAGGTAACGAATTCGGCGCGGTTACCGGTCGTCCGCGTCGTTGCGGTTGGTTTGACGCGGCGGCAATTCGTCGCGCTATTCAACTGAACTCCATTTCCGGTTTCTGCATGACCAAATTAGACGTGCTGGACGGTTTCGATGAAGTGAAAATCTGCGTCGGCTACAAAATGCCGAACGGCGACATCGTGGAATACGCCCCGCTCGCCGCGAAAGATTGGGAAg                                                                                     >  1:676215/1‑251 (MQ=255)                                                                                                                                                            gaAACAGGTGCTGAAATCGCCCGTAAAGGTAACGAATTCGGCGCGGTTACCGGTCGTCCGCGTCGTTGCGGTTGGTTTGACGCGGCGGCAATTCGTCGCGCTATTCAACTGAACTCCATTTCCGGTTTCTGCATGACCAAATTAGACGTGCTGGACGGTTTCGATGAAGTGAAAATCTGCGTCGGCTACAAAATGCCGAACGGTGACATCGTGGAATACGCCCCGCTCGCCGCGAAAGATTGGGAAGGCGt                                                                                 >  1:542607/1‑251 (MQ=255)                                                                                                                                                                   gTGCTGAAATCGCCCGTAAAGGTAACGAATTCGGCGCGGTTACCGGTCGTCCGCGTCGTTGCGGTTGGTTTGACGCGGCGGCAATTCGTCGCGCTATTCAACTGAACTCCATTTCCGGTTTCTGCATGACCAAATTAGACGTGCTGGACGGTTTCGATGAAGTGAAAATCTGCGTCGGCTACAAAATGCCGAACGGTGACATCGTGGAATACGCCCCGCTCGCCGCGAAAGATTGGGAAGGCGTTGAAc                                                                            <  1:603771/249‑1 (MQ=255)                                                                                                                                                                   gTGCTGAAATCGCCCGTAAAGGTAACGAATTCGGCGCGGTTACCGGTCGTCCGCGTCGTTGCGGTTGGTTTGACGCGGCGGCAATTCGTCGCGCTATTCAACTGAACTCCATTTCCGGTTTCTGCATGACCAAATTAGACGTGCTGGACGGTTTCGATGAAGTGAAAATCTGCGTCGGCTACAAAATGCCGAACGGTGACATCGTGGAATACGCCCCGCTCGCCGCGAAAGATTGGGAAGGCGTTGAAc                                                                            <  1:177296/249‑1 (MQ=255)                                                                                                                                                                   gTGCTGAAATCGCCCGTAAAGGTAACGAATTCGGCGCGGTTACCGGTCGTCCGCGTCGTTGCGGTTGGTTTGACGCGGCGGCAATTCGTCGCGCTATTCAACTGAACTCCATTTCCGGTTTCTGCATGACCAAATTAGACGTGCTGGACGGTTTCGATGAAGTGAAAATCTGCGTCGGCTACAAAATGCCGAACGGTGACATCGTGGAATACGCCCCGCTCGCCGCGAAAGATTGGGAAGGCGTTGAACCg                                                                          >  1:461752/1‑251 (MQ=255)                                                                                                                                                                        gAAATCGCCCGTAAAGGTAACGAATTCGGCGCGGTTACCGGTCGTCCGCGTCGTTGCGGTTGGTTTGACGCGGCGGCAATTCGTCGCGCTATTCAACTGAACTCCATTTCCGGTTTCTGCATGACCAAATTAGACGTGCTGGACGGTTTCGATGAAGTGAAAATCTGCGTCGGCTACAAAATGCCGAACGGTGACATCGTGGAATACGCCCCGCTCGCCGCGAAAGATTGGGAAGGCGTTGAACCGATTTa                                                                     >  1:252369/1‑251 (MQ=255)                                                                                                                                                                           aTCGCCCGTAAAGGTAACGAATTCGGCGCGGTTACCGGTCGTCCGCGTCGTTGCGGTTGGTTTGACGCGGCGGCAATTCGTCGCGCTATTCAACTGAACTCCATTTCCGGTTTCTGCATGACCAAATTAGACGTGCTGGACGGTTTCGATGAAGTGAAAATCTGCGTCGGCTACAAAATGCCGAACGGTGACATCGTGGAATACGCCCCGCTCGCCGCGAAAGATTGGGAAGGCGTTGAACCGATTTACGa                                                                  <  1:366622/251‑1 (MQ=255)                                                                                                                                                                            tCGCCCGTAAAGGTAACGAATTCGGCGCGGTTACCGGTCGTCCGCGTCGTTGCGGTTGGTTTGACGCGGCGGCAATTCGTCGCGCTATTCAACTGAACTCCATTTCCGGTTTCTGCATGACCAAATTAGACGTGCTGGACGGTTTCGATGAAGTGAAAATCTGCGTCGGCTACAAAATGCCGAACGGTGACATCGTGGAATACGCCCCGCTCGCCGCGAAAGATTGGGAAGGCGTTGAACCGATTTACGaa                                                                 <  1:414575/251‑1 (MQ=255)                                                                                                                                                                               cccGTAAAGGTAACGAATTCGGCGCGGTTACCGGTCGTCCGCGTCGTTGCGGTTGGTTTGACGCGGCGGCAATTCGTCGCGCTATTCAACTGAACTCCATTTCCGGTTTCTGCATGACCAAATTAGACGTGCTGGACGGTTTCGATGAAGTGAAAATCTGCGTCGGCTACAAAATGCCGAACGGTGACATCGTGGAATACGCCCCGCTCGCCGCGAAAGATTGGGAAGGCGTTGAACCGATTTACGAAAcc                                                              <  1:182909/251‑1 (MQ=255)                                                                                                                                                                                  gTAAAGGTAACGAATTCGGCGCGGTTACCGGTCGTCCGCGTCGTTGCGGTTGGTTTGACGCGGCGGCAATTCGCCGCGCTATTCaaat                                                                                                                                                                                                                              >  1:272414/1‑86 (MQ=255)                                                                                                                                                                                        gTAACGAATTCGGCGCGGTTACCGGTCGTCCGCGTTGTTGCGGTTGGTTTGACGCGGCGGCAATTCGTCGCGCTATTCAACTGAACTCCATTTCCGGTTTCTGCATGACCAAATTAGACGTGCTGGACGGTTTCGATGAAGTGAAAATc                                                                                                                                                           >  1:834325/1‑149 (MQ=255)                                                                                                                                                                                              aaTTCGGCGCGGTTACCGGTCGTCCGCGTCGTTGCGGTTGGTTTGACGCGGCGGCAATTCGTCGCGCTATTCAACTGAACTCCATTTCCGGTTTCTGCATGACCAAATTAGACGTGCTGGACGGTTTCGATGAAGTGAAAATCTGCGTCGGCTACAAAATGCCGAACGGTGACATCGTGGAATACGCCCCGCTCGCCGCGAAAGATTGGGAAGGCGTTGAACCGATTTACGAAACCTTGCCGGGCTGgaaa                                               <  1:898461/251‑1 (MQ=255)                                                                                                                                                                                               aTTCGGCGCGGTTACCGGTCGTCCGCGTCGTTGCGGTTGGTTTGACGCGGCGGCAATTCGTCGCGCTATTCAACTGAACTCCATTTCCGGTTTCTGCATGACCAAATTAGACGTGCTGGACGGTTTCGATGAAGTGAAAATCTGCGTCGGCTACAAAATGCCGAACGGTGACATCGTGGAATACGCCCCGCTCGCCGCGAAAGATTGGGAAGGCGTTGAACCGATTTACGAAACCTTGCCGGGCTGgaaag                                              <  1:204381/251‑1 (MQ=255)                                                                                                                                                                                                  cGGCGCGGTTACCGGTCGTCCGCGTCGTTGCGGTTGGTTTGACGCGGCGGCAATTCGTCGCGCTATTCAACTGAACTCCATTTCCGGTTTCTGCATGACCAAATTAGACGTGCTGGACGGTTTCGATGAAGTGAAAATCTGCGTCGGCTACAAAATGCCGAACGGTGACATCGTGGAATACGCCCCGCTCGCCGCGAAAGATTGGGAAGGCGTTGAACCGATTTACGAAACCTTGCCGGGCTGgaaagaaa                                           <  1:462/251‑1 (MQ=255)                                                                                                                                                                                                       cgGTTACCGGTCGTCCGCGTCGTTGCGGTTGGTTTGACGCGGCGGCAATTCGTCGCGCTATTCAACTGAACTCCATTTCCGGTTTCTGCATGACCAAATTAGACGTGCTGGACGGTTTCGATGAAGTGAAAATCTGCGTCGGCTACAAAATGCCGAACGGTGACATCGTGGAATACGCCCCGCTCGCCGCGAAAGATTGGGAAGGCGTTGAACCGATTTACGAAACCTTGCCGGGCTGGAAAGAAAACAcc                                      <  1:200047/251‑1 (MQ=255)                                                                                                                                                                                                         gTTACCGGTCGTCCGCGTCGTTGCGGTTGGTTTGACGCGGCGGCAATTCGTCGCGCTATTCAACTGAACTCCATTTCCGGTTTCTGCATGACCAAATTAGACGTGCTGGACGGTTTCGATGAAGTGAAAATCTGCGTCGGCTACAAAATGCCGAACGGTGACATCGTGGAATACGCCCCGCTCGCCGCGAAAGATTGGGAAGGCGTTGAACCGATTTACGAAACCTTGCCGGGCTGGAAAGAAAACACCtt                                    >  1:48276/1‑251 (MQ=255)                                                                                                                                                                                                         gTTACCGGTCGTCCGCGTCGTTGCGGTTGGTTTGACGCGGCGGCAATTCGTCGCGCTATTCAACTGAACTCCATTTCCGGTTTCTGCATGACCAAATTAGACGTGCTGGACGGTTTCGATGAAGTGAAAATCTGCGTCGGCTACAAAATGCCGAACGGTGACATCGTGGAATACGCCCCGCTCGCCGCGAAAGATTGGGAAGGCGTTGAACCGATTTACGAAACCTTGCCGGGCTGGAAAGAAAACACCtt                                    >  1:816946/1‑251 (MQ=255)                                                                                                                                                                                                                gtcgtcCGCGTCGTTGCGGTTGGTTTGACGCGGCGGCAATTCGTCGCGCTATTCAACTGAACTCCATTTCCGGTTTCTGCATGACCAAATTAGACGTGCTGGACGGTTTCGATGAAGTGAAAATCTGCGTCGGCTACAAAATGCCGAACGGTGACATCGTGGAATACGCCCCGCTCGCCGCGAAAGATTGGGAAGGCGTTGAACCGATTTACGAAACCTTGCCGGGCTGGAAAGAAAACACCTTCGGCAt                              <  1:419538/250‑1 (MQ=255)                                                                                                                                                                                                                    tcCGCGTCGTTGCGGTTGGTTTGACGCGGCGGCAATTCGTCGCGCTATTCAACTGAACTCCATTTCCGGTTTCTGCATGACCAAATTAGACGTGCTGGACGGTTTCGATGAAGTGAAAATCTGCGTCGGCTACAAAATGCCGAACGGTGACATCGTGGAATACGCCCCGCTCGCCGCGAAAGATTGGGAAGGCGTTGAACCGATTTACGAAACCTTGCCGGGCTGGAAAGAAAACACCTTCGGCATCACCg                         <  1:845570/251‑1 (MQ=255)                                                                                                                                                                                                                          tcgtTGCGGTTGGTTTGACGCGGCGGCAATTCGTCGCGCTATTCAACTGAACTCCATTTCCGGTTTCTGCATGACCAAATTAGACGTGCTGGACGGTTTCGATGAAGTGAAAATCTGCGTCGGCTACAAAATGCCGAACGGTGACATCGTGGAATACGCCCCGCTCGCCGCGAAAGATTGGGAAGGCGTTGAAc                                                                            <  1:82815/194‑1 (MQ=255)                                                                                                                                                                                                                          tcgtTGCGGTTGGTTTGACGCGGCGGCAATTCGTCGCGCTATTCAACTGAACTCCATTTCCGGTTTCTGCATGACCAAATTAGACGTGCTGGACGGTTTCGATGAAGTGAAAATCTGCGTCGGCTACAAAATGCCGAACGGTGACATCGTGGAATACGCCCCGCTCGCCGCGAAAGATTGGGAAGGCGTTGAACCGATTTACGAAACCTTGCCGGGCTGGAAAGAAAACACCTTCGGCATCACCGATGTTa                   >  1:633584/1‑251 (MQ=255)                                                                                                                                                                                                                             ttGCGGTTGGTTTGACGCGGCGGCAATTCGTCGCGCTATTCAACTGAACTCCATTTCCGGTTTCTGCATGACCAAATTAGACGTGCTGGACGGTTTCGATGAAGTGAAAATCTGCGTCGGCTACAAAATGCCGAACGGTGACATCGTGGAATACGCCCCGCTCGCCGCGAAAGATTGGGAAGGCGTTGAACCGATTTACGAAACCTTGCCGGGCTGGAAAGAAAACACCTTCGGCATCACCGATGTTAAcc                <  1:362913/251‑1 (MQ=255)                                                                                                                                                                                                                                cGGTTGGTTTGACGCGGCGGCAATTCGTCGCGCTATTCAACTGAACTCCATTTCCGGTTTCTGCATGACCAAATTAGACGTGCTGGACGGTTTCGATGAAGTGAAAATCTGCGTCGGCTACAAAATGCCGAACGGTGACATCGTGGAATACGCCCCGCTCGCCGCGAAAGATTGGGAAGGCGTTGAACCGATTTACGAAACCTTGCCGGGCTGGAAAGAAAACACCTTCGGCATCACCGATGTTAACCaa              <  1:826572/250‑1 (MQ=255)                                                                                                                                                                                                                                   ttggttTGACGCGGCGGCAATTCGTCGCGCTATTCAACTGAACTCCATTTCCGGTTTCTGCATGACCAAATTAGACGTGCTGGACGGTTTCGATGAAGTGAAAATCTGCGTCGGCTACAAAATGCCGAACGGTGACATCGTGGAATACGCCCCGCTCGCCGCGAAAGATTGGGAAGGCGTTGAACCGATTTACGAAACCTTGCCGGGCTGGAAAGAAAACACCTTCGGCATCACCGATGTTAACCAATTg           <  1:236070/250‑1 (MQ=255)                                                                                                                                                                                                                                        ttGACGCGGCGGCAATTCGTCGCGCTATTCAACTGAACTCCATTTCCGGTTTCTGCATGACCAAATTAGACGTGCTGGACGGTTTCGAT‑‑‑GTGAAAATCTGCGTCGGCTACAAAATGCCGAACGGTGACATCGTGGAATACGCCCCGCTCGCCGCGAAAGATTGGGAAGGCGTTGAACCGATTTACGAAACCTTGCCGGGCTGGAAAGAAAACACCTTCGGCATCACCGATGTTAACCAATTGCCGCAAAcc  <  1:644946/251‑1 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                     GCACCTATCCGTATGTAACCAGCTCCAACACCACCGCCGGCGGCGTAGCGACAGGCTCCGGTTTCGGTCCGCGCAATCTGGATTATGTGTTGGGAATCATCAAAGCCTACTGCACCCGCGTGGGCGGCGGTCCGTTCACTACGGAATTATTTGATGAAACAGGTGCTGAAATCGCCCGTAAAGGTAACGAATTCGGCGCGGTTACCGGTCGTCCGCGTCGTTGCGGTTGGTTTGACGCGGTGGCAATTCGTCGCGCTATTCAACTGAACTCCATTTCCGGTTTCTGCATGACCAAATTAGACGTGCTGGACGGTTTCGATGAAGTGAAAATCTGCGTCGGCTACAAAATGCCGAACGGTGACATCGTGGAATACGCCCCGCTCGCCGCGAAAGATTGGGAAGGCGTTGAACCGATTTACGAAACCTTGCCGGGCTGGAAAGAAAACACCTTCGGCATCACCGATGTTAACCAATTGCCGCAAACC  >  1/1347319‑1347803

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑