Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,345,718	A→G	L51L (TTA→TTG)	unknown →	tRNA ho5U(34) carboxymethyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,345,718	0	A	G	100.0%	218.5 / NA	77	L51L (TTA→TTG)	unknown	tRNA ho5U(34) carboxymethyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (34/43);  total (34/43)

TCGTTGCTGTCGGCAAAACCGGATGCGGAAATTACCGACACCTTTGACAAAGCCCAAAAGGCGTACAAAGAAGCTTTATAAGCGAGATAGCAAAATGATCGATTTTCGTCCTTTTTATCAACAAATCGCCACCACCAACCTTTCGCCATGGCTTGAAACGCTGCCCCTGCAACTCAAACAATGGCAGCAACAAACCCATGGCGACTATGCCAAATGGGCAAAAGTCTTGGAATTTCTACCGCACTTAGCAGCATCCCGCATTGACTTGAAAAGTGCGGTCAAATCTGAAAGCGTTTCTCCTCTTTCCGAAGGCGAACGGCAACGTATTGTGCACCACCTCAAACAGCTCATGCCGTGGCGTAAAGGCCCCTATGATTTGCTTGGCATTCATGTGGATTGCGAATGGCGCTCCGATTTCAAATGGGATCGCGTGCTGCCCCATCTTGCACCGTTGCAGGATCGCATTATTTTAGATG  >  1/1345472‑1345947                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                      tCGTTGCTGTCGGCAAAACCGGATGCGGAAATTACCGACACCTTTGACAAAGCCCAAAAGGCGTACAAAGAAGCTTTATAAGCGAGATAGCAAAATGATCGATTTTCGTCCTTTTTATCAACAAATCGCCACCACCAACCTTTCGCCATGGCTTGAAACGCTGCCCCTGCAACTCAAACAATGGCAGCAACAAACCCATGGCGACTATGCCAAATGGGCAAAAGTCTTGGAATTTCTACCGCACTTggcag                                                                                                                                                                                                                                   <  1:804935/251‑1 (MQ=255)                  aCCGGATGCGGAAATTACCGACACCTTTGACAAAGCCCAAAAGGCGTACAAAGAAGCTTTATAAGCGAGATAGCAAAATGATCGATTTTCGTCCTTTTTATCAACAAATCGCCACCACCAACCTTTCGCCATGGCTTGAAACGCTGCCCCTGCAACTCAAACAATGGCAGCAACAAACCCATGGCGACTATGCCAAATGGGCAAAAGTCTTGGAATTTCTACCGCACTTGGCAGCATCCCGCATTGACTTg                                                                                                                                                                                                                  >  1:900804/1‑251 (MQ=255)                      gATGCGGAAATTACCGACACCTTTGACAAAGCCCAAAAGGCGTACAAAGAAGCTTTATAAGCGAGATAGCAAAATGATCGATTTTCGTCCTTTTTATCAACAAATCGCCACCACCAACCTTTCGCCATGGCTTGAAACGCTGCCCCTGCAACTCAAACAATGGCAGCAACAAACCCATGGCGACTATGCCAAATGGGCAAAAGTCTTGGAATTTCTACCGCACTTGGCAGCATCCCGCATTGACTTGaaa                                                                                                                                                                                                               <  1:896538/250‑1 (MQ=255)                      gATGCGGAAATTACCGACACCTTTGACAAAGCCCAAAAGGCGTACAAAGAAGCTTTATAAGCGAGATAGCAAAATGATCGATTTTCGTCCTTTTTATCAACAAATCGCCACCACCAACCTTTCGCCATGGCTTGAAACGCTGCCCCTGCAACTCAAACAATGGCAGCAACAAACCCATGGCGACTATGCCAAATGGGCAAAAGTCTTGGAATTTCTACCGCACTTGGCAGCATCCCGCATTGACTTGaaa                                                                                                                                                                                                               <  1:822055/250‑1 (MQ=255)                      gATGCGGAAATTACCGACACCTTTGACAAAGCCCAAAAGGCGTACAAAGAAGCTTTATAAGCGAGATAGCAAAATGATCGATTTTCGTCCTTTTTATCAACAAATCGCCACCACCAACCTTTCGCCATGGCTTGAAACGCTGCCCCTGCAACTCAAACAATGGCAGCAACAAACCCATGGCGACTATGCCAAATGGGCAAAAGTCTTGGAATTTCTACCGCACTTGGCAGCATCCCGCATTGACTTGaaa                                                                                                                                                                                                               <  1:361881/250‑1 (MQ=255)                      gATGCGGAAATTACCGACACCTTTGACAAAGCCCAAAAGGCGTACAAAGAAGCTTTATAAGCGAGATAGCAAAATGATCGATTTTCGTCCTTTTTATCAACAAATCGCCACCACCAACCTTTCGCCATGGCTTGAAACGCTGCCCCTGCAACTCAAACAATGGCAGCAACAAACCCATGGCGACTATGCCAAATGGGCAAAAGTCTTGGAATTTCTACCGCACTTGGCAGCATCCCGCATTGACTTGaaa                                                                                                                                                                                                               <  1:686019/250‑1 (MQ=255)                      gATGCGGAAATTACCGACACCTTTGACAAAGCCCAAAAGGCGTACAAAGAAGCTTTATAAGCGAGATAGCAAAATGATCGATTTTCGTCCTTTTTATCAACAAATCGCCACCACCAACCTTTCGCCATGGCTTGAAACGCTGCCCCTGCAACTCAAACAATGGCAGCAACAAACCCATGGCGACTATGCCAAATGGGCAAAAGTCTTGGAATTTCTACCGCACTTGGCAGCATCCCGCATTGACTTGaaa                                                                                                                                                                                                               <  1:653468/250‑1 (MQ=255)                                  aCCGACACCTTTGACAAAGCCCAAAAGGCGTACAAAGAAGCTTTATAAGCGAGATAGCAAAATGATCGATTTTCGTCCTTTTTATCAACAAATCGCCACCACCAACCTTTCGCCATGGCTTGAAACGCTGCCCCTGCAACTCAAACAATGGCAGCAACAAACCCATGGCGACTATGCCAAATGGGCAAAAGTCTTGGAATTTCTACCGCACTTGGCAGCATCCCGCATTGACTTGAAAAGTGCGGTCAAAt                                                                                                                                                                                                  >  1:564496/1‑251 (MQ=255)                                   ccGACACCTTTGACAAAGCCCAAAAGGCGTACAAAGAAGCTTTATAAGCGAGATAGCAAAATGATCGATTTTCGTCCTTTTTATCAACAAATCGCCACCACCAACCTTTCGCCATGGCTTGAAACGCTGCCCCTGCAACTCAAACAATGGCAGCAACAAACCCATGGCGACTATGCCAAATGGGCAAAAGTCTTGGAATTTCTACCGCACTTGGCAGCATCCCGCATTGACTTGAAAAGTGCGGTCAAATc                                                                                                                                                                                                 <  1:281299/251‑1 (MQ=255)                                        acCTTTGACAAAGCCCAAAAGGCGTACAAAGAAGCTTTATAAGCGAGATAGCAAAATGATCGATTTTCGTCCTTTTTATCAACAAATCGCCACCACCAACCTTTCGCCATGGCTTGAAACGCTGCCCCTGCAACTCAAACAATGGCAGCAACAAACCCATGGCGACTATGCCAAATGGGCAAAAGTCTTGGAATTTCTACCGCACTTGGCAGCATCCCGCATTGACTTGAAAAGTGCGGTCAAATCTGaaa                                                                                                                                                                                            >  1:669408/1‑251 (MQ=255)                                         ccTTTGACAAAGCCCAAAAGGCGTACAAAGAGGCTTTATAAGCGAGATAGCAAAATGATCGATTTTCGTCCTTTTTATCAACAAATCGCCACCACCAACCTTTCGCCATGGCTTGAAACGCTGCCCCTGCAACTCAAACAATGGCAGCAACAAACCCATGGCGACTATGCCAAATGGGCAAAAGTCTTGGAATTTCTACCGCACTTGGCAGCATCCCGCATTGACTTGaaa                                                                                                                                                                                                               <  1:316029/231‑1 (MQ=255)                                         ccTTTGACAAAGCCCAAAAGGCGTACAAAGAAGCTTTATAAGCGAGATAGCAAAATGATCGATTTTCGTCCTTTTTATCAACAAATCGCCACCACCAACCTTTCGCCATGGCTTGAAACGCTGCCCCTGCAACTCAAACAATGGCAGCAACAAACCCATGGCGACTATGCCAAATGGGCAAAAGTCTTGGAATTTCTACCGCACTTGGCAGCATCCCGCATTGACTTGAAAAGTGCGGTCAAATCTGAAAg                                                                                                                                                                                           >  1:198412/1‑251 (MQ=255)                                         ccTTTGACAAAGCCCAAAAGGCGTACAAAGAAGCTTTATAAGCGAGATAGCAAAATGATCGATTTTCGTCCTTTTTATCAACAAATCGCCACCACCAACCTTTCGCCATGGCTTGAAACGCTGCCCCTGCAACTCAAACAATGGCAGCAACAAACCCATGGCGACTATGCCAAATGGGCAAAAGTCTTGGAATTTCTACCGCACTTGGCAGCATCCCGCATTGACTTGAAAAGTGCGGTCAAATCTGAAAg                                                                                                                                                                                           >  1:540559/1‑251 (MQ=255)                                                    gCCCAAAAGGCGTACAAAGAAGCTTTATAAGCGAGATAGCAAAATGATCGATTTTCGTCCTTTTTATCAACAAATCGCCACCACCAACCTTTCGCCATGGCTTGAAACGCTGCCCCTGCAACTCAAACAATGGCAGCAACAAACCCATGGCGACTATGCCAAATGGGCAAAAGTCTTGGAATTTCTACCGCACTTGGCAGCATcc                                                                                                                                                                                                                              <  1:73379/205‑1 (MQ=255)                                                    gCCCAAAAGGCGTACAAAGAAGCTTTATAAGCGAGATAGCAAAATGATCGATTTTCGTCCTTTTTATCAACAAATCGCCACCACCAACCTTTCGCCATGGCTTGAAACGCTGCCCCTGCAACTCAAACAATGGCAGCAACAAACCCATGGCGACTATGCCAAATGGGCAAAAGTCTTGGAATTTCTACCGCACTTGGCAGCATCCCGCAt                                                                                                                                                                                                                         >  1:537151/1‑210 (MQ=255)                                                    gCCCAAAAGGCGTACAAAGAAGCTTTATAAGCGAGATAGCAAAATGATCGATTTTCGTCCTTTTTATCAACAAATCGCCACCACCAACCTTTCGCCATGGCTTGAAACGCTGCCCCTGCAACTCAAACAATGGCAGCAACAAACCCATGGCGACTATGCCAAATGGGCAAAAGTCTTGGAATTTCTACCGCACTTGGCAGCATCCCGCATTGACTTGAAAAGTGCGGTCAAATCTGAAAGCGTTTctcct                                                                                                                                                                                 >  1:187044/1‑250 (MQ=255)                                                    gCCCAAAAGGCGTACAAAGAAGCTTTATAAGCGAGATAGCAAAATGATCGATTTTCGTCCTTTTTATCAACAAATCGCCACCACCAACCTTTCGCCATGGCTTGAAACGCTGCCCCTGCAACTCAAACAATGGCAGCAACAAACCCATGGCGACTATGCCAAATGGGCAAAAGTCTTGGAATTTCTACCGCACTTGGCAGCATCCCGCATTGACTTGAAAAGTGCGGTCAAATCTGAAAGCGTTTctcct                                                                                                                                                                                 >  1:373850/1‑250 (MQ=255)                                                    gCCCAAAAGGCGTACAAAGAAGCTTTATAAGCGAGATAGCAAAATGATCGATTTTCGTCCTTTTTATCAACAAATCGCCACCACCAACCTTTCGCCATGGCTTGAAACGCTGCCCCTGCAACTCAAACAATGGCAGCAACAAACCCATGGCGACTATGCCAAATGGGCAAAAGTCTTGGAATTTCTACCGCACTTGGCAGCATCCCGCATTGACTTGAAAAGTGCGGTCAAATCTGAAAGCGTTTctcct                                                                                                                                                                                 >  1:273502/1‑250 (MQ=255)                                                     cccAAAAGGCGTACAAAGAAGCTTTATAAGCGAGATAGCAAAATGATCGATTTTCGTCCTTTTTATCAACAAATCGCCACCACCAACCTTTCGCCATGGCTTGAAACGCTGCCCCTGCAACTCAAACAATGGCAGCAACAAACCCATGGCGACTATGCCAAATGGGCAAAAGTCTTGGAATTTCTACCGCACTTGGCAGCATCCCGCATTGACTTGAAAAGTGCGGTCAAATCTGAAAGCGTTTctcctc                                                                                                                                                                                <  1:147656/250‑1 (MQ=255)                                                     cccAAAAGGCGTACAAAGAAGCTTTATAAGCGAGATAGCAAAATGATCGATTTTCGTCCTTTTTATCAACAAATCGCCACCACCAACCTTTCGCCATGGCTTGAAACGCTGCCCCTGCAACTCAAACAATGGCAGCAACAAACCCATGGCGACTATGCCAAATGGGCAAAAGTCTTGGAATTTCTACCGCACTTGGCAGCATCCCGCATTGACTTGAAAAGTGCGGTCAAATCTGAAAGCGTTTctcct                                                                                                                                                                                 >  1:301006/1‑249 (MQ=255)                                                             gCGTACAAAGAAGCTTTATAAGCGAGATAGCAAAATGATCGATTTTCGTCCTTTTTATCAACAAATCGCCACCACCAACCTTTCGCCATGGCTTGAAACGCTGCCCCTGCAACTCAAACAATGGCAGCAACAAACCCATGGCGACTATGCCAAATGGGCAAAAGTCTTGGAATTTCTACCGCACTTGGCAGCATCCCGCAt                                                                                                                                                                                                                         >  1:762026/1‑201 (MQ=255)                                                                  cAAAGAAGCTTTATAAGCGAGATAGCAAAATGATCGATTTTCGTCCTTTTTATCAACAAATCGCCACCACCAACCTTTCGCCATGGCTTGAAACGCTGCCCCTGCAACTCAAACAATGGCAGCAACAAACCCATGGCGACTATGCCAAATGGGCAAAAGTCTTGGAATTTCTACCGCACTTGGCAGCATCCCGCATTGACTTGaaa                                                                                                                                                                                                               >  1:638873/1‑206 (MQ=255)                                                                         gCTTTATAAGCGAGATAGCAAAATGATCGATTTTCGTCCTTTTTATCAACAAATCGCCACCACCAACCTTTCGCCATGGCTTGAAACGCTGCCCCTGCAACTCAAACAATGGCAGCAACAAACCCATGGCGACTATGCCAAATGGGCAAAAGTCTTGGAATTTCTACCGCACTTGGCAGCATCCCGCATTGACTTGAAAAGTGCGGTCAAATCTGAAAGCGTTTCTCCTCTTTCCGAAGGCGAACGGCaa                                                                                                                                                            >  1:745497/1‑250 (MQ=255)                                                                          cTTTATAAGCGAGATAGCAAAATGATCGATTTTCGTCCTTTTTATCAACAAATCGCCACCACCAACCTTTCGCCATGGCTTGAAACGCTGCCCCTGCAACTCAAACAATGGCAGCAACAAACCCATGGCGACTATGCCAAATGGGCAAAAGTCTTGGAATTTCTACCGCACTTGGCAGCATCCCGCATTGACTTGAAAAGTGCGGTCAAATCTGAAAGCGTTTCTCCTCTTTCCGAAGGCGAACGGCAACg                                                                                                                                                          >  1:541677/1‑251 (MQ=255)                                                                            ttATAAGCGAGATAGCAAAATGATCGATTTTCGTCCTTTTTATCAACAAATCGCCACCACCAACCTTTCGCCATGGCTTGAAACGCTGCCCCTGCAACTCAAACAATGGCAGCAACAAACCCATGGCGACTATGCCAAATGGGCAAAAGTCTTGGAATTTCTACCGCACTTGGCAGCATCCCGCATTGACTTGAAAAGTGCGGTCAAATCTGAAAGCGTTTctcctc                                                                                                                                                                                <  1:764385/227‑1 (MQ=255)                                                                            ttATAAGCGAGATAGCAAAATGATCGATTTTCGTCCTTTTTATCAACAAATCGCCACCACCAACCTTTCGCCATGGCTTGAAACGCTGCCCCTGCAACTCAAACAATGGCAGCAACAAACCCATGGCGACTATGCCAAATGGGCAAAAGTCTTGGAATTTCTACCGCACTTGGCAGCATCCCGCATTGACTTGAAAAGTGCGGTCAAATCTGAAAGCGTTTCTCCTCTTTCCGAAGGCGAACGGCAACg                                                                                                                                                          >  1:338400/1‑249 (MQ=255)                                                                                   cGAGATAGCAAAATGATCGATTTTCGTCCTTTTTATCAACAAATCGCCACCACCAACCTTTCGCCATGGCTTGAAACGCTGCCCCTGCAACTCAAACAATGGCAGCAACAAACCCATGGCGACTATGCCAAATGGGCAAAAGTCTTGGAATTTCTACCGCACTTGGCAGCATCCCGCATTGACTTGAAAAGTGCGGTCAAATCTGAAAGCGTTTCTCCTCTTTCCGAAGGCGAACGGCAACGTATTGTGc                                                                                                                                                  <  1:448265/250‑1 (MQ=255)                                                                                     agaTAGCAAAATGATCGATTTTCGTCCTTTTTATCAACAAATCGCCACCACCAACCTTTCGCCATGGCTTGAAACGCTGCTCCTGCAACTCAAACAATGGCAGCAACAAACCCATGGCGACTATGCCAAATGGGCAAAAGTCTTGGAATTTCTACCGCACTTGGCAGCATCCCGCATTGACTTGAAAAGTGCGGTCAAATCTGAAAGCGTTTCTCCTCTTTCCGAAGGCGAACGGCAACGTATTGTGcac                                                                                                                                                <  1:446111/250‑1 (MQ=255)                                                                                     agaTAGCAAAATGATCGATTTTCGTCCTTTTTATCAACAAATCGCCACCACCAACCTTTCGCCATGGCTTGAAACGCTGCCCCTGCAACTCAAACAATGGCAGCAACAAACCCATGGCGACTATGCCAAATGGGCAAAAGTCTTGGAATTTCTACCGCACTTGGCAGCATCCCGCATTGACTTGAAAAGTGCGGTCAAATCTGAAAGCGTTTCTCCTCTTTCCGAAGGCGAACGGCAACGTATTGTGcac                                                                                                                                                <  1:223412/250‑1 (MQ=255)                                                                                       aTAGCAAAATGATCGATTTTCGTCCTTTTTATCAACAAATCGCCACCACCAACCTTTCGCCATGGCTTGAAACGCTGCCCCTGCAACTCAAACAATGGCAGCAACAAACCCATGGCGACTATGCCAAATGGGCAAAAGTCTTGGAATTTCTACCGCACTTGGCAGCATCCCGCATTGACTTGAAAAGTGCGGTCAAATCTGAAAGCGTTTCTCCTCTTTCCGAAGGCGAACGGCAACGTATTGTGcaccac                                                                                                                                             <  1:149671/251‑1 (MQ=255)                                                                                       aTAGCAAAATGATCGATTTTCGTCCTTTTTATCAACAAATCGCCACCACCAACCTTTCGCCATGGCTTGAAACGCTGCCCCTGCAACTCAAACAATGGCAGCAACAAACCCATGGCGACTATGCCAAATGGGCAAAAGTCTTGGAATTTCTACCGCACTTGGCAGCATCCCGCATTGACTTGAAAAGTGCGGTCAAATCTGAAAGCGTTTCTCCTCTTTCCGAAGGCGAACGGCAACGTATTGTGcaccac                                                                                                                                             <  1:753683/251‑1 (MQ=255)                                                                                       aTAGCAAAATGATCGATTTTCGTCCTTTTTATCAACAAATCGCCACCACCAACCTTTCGCCATGGCTTGAAACGCTGCCCCTGCAACTCAAACAATGGCAGCAACAAACCCATGGCGACTATGCCAAATGGGCAAAAGTCTTGGAATTTCTACCGCACTTGGCAGCATCCCGCATTGACTTGAAAAGTGCGGTCAAATCTGAAAGCGTTTCTCCTCTTTCCGAAGGCGAACGGCAACGTATTGTGc                                                                                                                                                  <  1:588312/246‑1 (MQ=255)                                                                                              aaTGATCGATTTTCGTCCTTTTTATCAACAAATCGCCACCACCAACCTTTCGCCATGGCTTGAAACGCTGCCCCTGCAACTCAAACAATGGCAGCAACAAACCCATGGCGACTATGCCAAATGGGCAAAAGTCTTGGAATTTCTACCGCACTTGGCAGCATCCCGCATTGACTTGAAAAGTGCGGTCAAATCTGAAAGCGTTTCTCCTCTTTCCGAAGGCGAACGGCAACGTATTGTGCACCACCTCAAAc                                                                                                                                      <  1:70617/251‑1 (MQ=255)                                                                                               aTGATCGATTTTCGTCCTTTTTATCAACAAATCGCCACCACCAACCTTTCGCCATGGCTTGAAACGCTGCCCCTGCAACTCAAACAATGGCAGCAACAAACCCATGGCGACTATGCCAAATGGGCAAAAGTCTTGGAATTTCTACCGCACTTGGCAGCATcc                                                                                                                                                                                                                              <  1:434774/162‑1 (MQ=255)                                                                                                tGATCGATTTTCGTCCTTTTTATCAACAAATCGCCACCACCAACCTTTCGCCATGGCTTGAAACGCTGCCCCTGCAACTCAAACAATGGCAGCAACAAACCCATGGCGACTATGCCAAATGGGCAAAAGTCTTGGAATTTCTACCGCACTTGGCAGCATCCCGCATTGACTTGAAAAGTGCGGTCAAATCTGAAAGCGTTTCTCCTCTTTCCGAAGGCGAACGGCAACGTATTGTGCACCACCTCAAACAg                                                                                                                                    <  1:636064/251‑1 (MQ=255)                                                                                                      aTTTTCGTCCTTTTTATCAACAAATCGCCACCACCAACCTTTCGCCATGGCTTGAAACGCTGCCCCTGCAACTCAAACAATGGCAGCAACAAACCCATGGCGACTATGCCAAATGGGCAAAAGTCTTGGAATTTCTACCGCACTTGGCAGCATCCCGCATTGACTTGAAAAGTGCGGTCAAATCTGAAAGCGTTTCTCCTCTTTCCGAAGGCGAACGGCAACGTATTGTGcac                                                                                                                                                <  1:636593/233‑1 (MQ=255)                                                                                                      aTTTTCGTCCTTTTTATCAACAAATCGCCACCACCAACCTTTCGCCATGGCTTGAAACGCTGCCCCTGCAACTCAAACAATGGCAGCAACAAACCCATGGCGACTATGCCAAATGGGCAAAAGTCTTGGAATTTCTACCGCACTTGGCAGCATCCCGCATTGACTTGAAAAGTGCGGTCAAATCTGAAAGCGTTTCTCCTCTTTCCGAAGGCGAACGGCAACGTATTGTGCACCACCTCAAACAGCTCATg                                                                                                                              >  1:647333/1‑251 (MQ=255)                                                                                                               cTTTTTATCAACAAATCGCCACCACCAACCTTTCGCCATGGCTTGAAACGCTGCCCCTGCAACTCAAACAATGGCAGCAACAAACCCATGGCGACTATGCCAAATGGGCAAAAGTCTTGGAATTTCTACCGCACTTGGCAGCATCCCGCATTGACTTGAAAAGTGCGGTCAAATCTGAAAGCGTTTCTCCTCTTTCCGAAGGCGAACGGCAACGTATTGTGCACCACCTCAAACAGCTCATGcc                                                                                                                            <  1:620114/244‑1 (MQ=255)                                                                                                                   ttATCAACAAATCGCCACCACCAACCTTTCGCCATGGCTTGAAACGCTGCCCCTGCAACTCAAACAATGGCAGCAACAAACCCATGGCGACTATGCCAAATGGGCAAAAGTCTTGGAATTTCTACCGCACTTGGCAGCATCCCGCATTGACTTGAAAAGTGCGGTCAAATCTGAAAGCGTTTCTCCTCTTTCCGAAGGCGAACGGCAACGTATTGTGCACCACCTCAAACAGCTCATGCCGTGGCGTAAAg                                                                                                                 <  1:823805/251‑1 (MQ=255)                                                                                                                      tCAACAAATCGCCACCACCAACCTTTCGCCATGGCTTGAAACGCTGCCCCTGCAACTCAAACAATGGCAGCAACAAACCCATGGCGACTATGCCAAATGGGCAAAAGTCTTGGAATTTCTACCGCACTTGGCAGCATCCCGCATTGACTTGAAAAGTGCGGTCAAATCTGAAAGCGTTTCTCCTCTTTCCGAAGGCGAACGGCAACGTATTGTGCACCACCTCAAACAGCTCATGCCGTGGCGTAAAGGcc                                                                                                              <  1:618999/251‑1 (MQ=255)                                                                                                                             aTCGCCACCACCAACCTTTCGCCATGGCTTGAAACGCTGCCCCTGCAACTCAAACAATGGCAGCAACAAACCCATGGCGACTATGCCAAATGGGCAAAAGTCTTGGAATTTCTACCGCACTTGGCAGCATCCCGCATTGACTTGAAAAGTGCGGTCAAATCTGAAAGCGTTTCTCCTCTTTCCGAAGGCGAACGGCAACGTATTGTGCACCACCTCAAACAGCTCATGCCGTGGCGTAAAGGCCCCTATGa                                                                                                       <  1:841725/251‑1 (MQ=255)                                                                                                                                  caccaccaACCTTTCGCCATGGCTTGAAACGCTGCCCCTGCAACTCAAACAATGGCAGCAACAAACCCATGGCGACTATGCCAAATGGGCAAAAGTCTTGGAATTTCTACCGCACTTGGCAGCATCCCGCATTGACTTGAAAAGTGCGGTCAAATCTGAAAGCGTTTCTCCTCTTTCCGAAGGCGAACGGCAACGTATTGTGCACCACCTCAAACAGCTCATGCCGTGGCGTAAAGGCCCCTATGATTTGc                                                                                                  <  1:81304/251‑1 (MQ=255)                                                                                                                                           ccTTTCGCCATGGCTTGAAACGCTGCCCCTGCAACTCAAACAATGGCAGCAACAAACCCATGGCGACTATGCCAAATGGGCAAAAGTCTTGGAATTTCTACCGCACTTGGCAGCATCCCGCATTGACTTGAAAAGTGCGGTCAAATCTGAAAGCGTTTCTCCTCTTTCCGAAGGCGAACGGCAACGTATTGTGCACCACCTCAAACAGCTCATGCCGTGGCGTAAAGGCCCCTATGATTTGCTTGGCAtt                                                                                          >  1:189679/1‑250 (MQ=255)                                                                                                                                                   cATGGCTTGAAACGCTGCCCCTGCAACTCAAACAATGGCAGCAACAAACCCATGGCGACTATGCCAAATGGGCAAAAGTCTTGGAATTTCTACCGCACTTGGCAGCATCCCGCATTGACTTGAAAAGTGCGGTCAAATCTGAAAGCGTTTCTCCTCTTTCCGAAGGCGAACGGCAACGTATTGTGCACCACCTCAAACAGCTCATGCCGTGGCGTAAAGGCCCCTATGATTTGCTTGGCATTCATGTGGAt                                                                                 <  1:301195/251‑1 (MQ=255)                                                                                                                                                      ggCTTGAAACGCTGCCCCTGCAACTCAAACAATGGCAGCAACAAACCCATGGCGACTATGCCAAATGGGCAAAAGTCTTGGAATTTCTACCGCACTTGGCAGCATCCCGCATTGACTTGAAAAGTGCGGTCAAATCTGAAAGCGTTTCTCCTCTTTCCGAAGGCGAACGGCAACGTATTGTGCACCACCTCAAACAGCTc                                                                                                                                 <  1:274668/200‑1 (MQ=255)                                                                                                                                                      ggCTTGAAACGCTGCCCCTGCAACTCAAACAATGGCAGCAACAAACCCATGGCGACTATGCCAAATGGGCAAAAGTCTTGGAATTTCTACCGCACTTGGCAGCATCCCGCATTGACTTGAAAAGTGCGGTCAAATCTGAAAGCGTTTCTCCTCTTTCCGAAGGCGAACGGCAACGTATTGTGCACCACCTCAAACAGCTCATGCCGTGGCGTAAAGGCCCCTATGATTTGCTTGGCATTCATGTGGATTg                                                                               >  1:246248/1‑250 (MQ=255)                                                                                                                                                       gCTTGAAACGCTGCCCCTGCAACTCAAACAATGGCAGCAACAAACCCATGGCGACTATGCCAAATGGGCAAAAGTCTTGGAATTTCTACCGCACTTGGCAGCATCCCGCATTGACTTGAAAAGTGCGGTCAAATCTGAAAGCGTTTCTCCTCTTTCCGAAGGCGAACGGCAACGTATTGTGCACCACCTCAAACAGCTCATGCCGTGGCGTAAAGGCCCCTATGATTTGCTTGGCATTCATGTGGATTGCg                                                                             >  1:117559/1‑251 (MQ=255)                                                                                                                                                                               cAAACAATGGCAGCAACAAACCCATGGCGACTATGCCAAATGGGCAAAAGTCTTGGAATTTCTACCGCACTTGGCAGCATCCCGCATTGACTTGAAAAGTGCGGTCAAATCTGAAAGCGTTTCTCCTCTTTCCGAAGGCGAACGGCAACGTAt                                                                                                                                                       >  1:560594/1‑153 (MQ=255)                                                                                                                                                                                   cAATGGCAGCAACAAACCCATGGCGACTATGCCAAATGGGCAAAAGTCTTGGAATTTCTACCGCACTTGGCAGCATCCCGCATTGACTTGAAAAGTGCGGTCAAATCTGAAAGCGTTTCTCCTCTTTCCGAAGGCGAACGGCaa                                                                                                                                                            <  1:675400/144‑1 (MQ=255)                                                                                                                                                                                     aTGGCAGCAACAAACCCATGGCGACTATGCCAAATGGGCAAAAGTCTTGGAATTTCTACCGCACTTGGCAGCATCCCGCATTGACTTGAAAAGTGCGGTCAAATCTGAAAGCGTTTCTCCTCTTTCCGAAGGCGAACGGCAACGTATTGTGCACCACCTCAAACAGCTCATGCCGTGGCGTAAAGGCCCCTATGATTTGCTTGGCATTCATGTGGATTGCGAATGGCGCTCCGATTTCAAATGGGATcgcg                                               <  1:418970/251‑1 (MQ=255)                                                                                                                                                                                      tGGCAGCAACAAACCCATGGCGACTATGCCAAATGGGCAAAAGTCTTGGAATTTCTACCGCACTTGGCAGCATCCCGCATTGACTTGAAAAGTGCGGTCAAATCTGAAAGCGTTTCTCCTCTTTCCGAAGGCGAACGGCAACGTATTGTGCACCACCTCAAACAGCTCATGCCGTGGCGTAAAGGCCCCTATGATTTGCTTGGCATTCATGTGGATTGCGAATGGCGCTCCGATTTCAAATGGGATCGCGt                                              >  1:15997/1‑251 (MQ=255)                                                                                                                                                                                       ggCAGCAACAAACCCATGGCGACTATGCCAAATGGGCAAAAGTCTTGGAATTTCTACCGCACTTGGCAGCATCCCGCATTGACTTGAAAAGTGCGGTCAAATCTGAAAGCGTTTCTCCTCTTTCCGAAGGCGAACGGCAACGTATTGTGCACCACCTCAAAc                                                                                                                                      <  1:378799/162‑1 (MQ=255)                                                                                                                                                                                         cagcaACAAACCCATGGCGACTATGCCAAATGGGCAAAAGTCTTGGAATTTCTACCGCACTTGGCAGCATCCCGCATTGACTTGAAAAGTGCGGTCAAATCTGAAAGCGTTTCTCCTCTTTCCGAAGGCGAACGGCAACGTATTGTGCACCACCTCAAACAGCTCATGCCGTGGCGTAAAGGCCCCTATGATTTGCTTGGCATTCATGTGGATTGCGAATGGCGCTCCGATTTCAAATGGGATCGCGtgc                                            <  1:639968/250‑1 (MQ=255)                                                                                                                                                                                         cagcaACAAACCCATGGCGACTATGCCAAATGGGCAAAAGTCTTGGAATTTCTACCGCACTTGGCAGCATCCCGCATTGACTTGAAAAGTGCGGTCAAATCTGAAAGCGTTTCTCCTCTTTCCGAAGGCGAACGGCAACGTATTGTGCACCACCTCAAACAGCTCATGCCGTGGCGTAAAGGCCCCTATGATTTGCTTGGCATTCATGTGGATTGCGAATGGCGCTCCGATTTCAAATGGGATCGCGtgc                                            <  1:30355/250‑1 (MQ=255)                                                                                                                                                                                         cagcaACAAACCCATGGCGACTATGCCAAATGGGCAAAAGTCTTGGAATTTCTACCGCACTTGGCAGCATCCCGCATTGACTTGAAAAGTGCGGTCAAATCTGAAAGCGTTTCTCCTCTTTCCGAAGGCGAACGGCAACGTATTGTGCACCACCTCAAACAGCTCATGCCGTGGCGTAAAGGCCCCTATGATTTGCTTGGCATTCATGTGGATTGCGAATGGCGCTCCGATTTCAAATGGGATCGCGtgc                                            <  1:662770/250‑1 (MQ=255)                                                                                                                                                                                           gcaACAAACCCATGGCGACTATGCCAAATGGGCAAAAGTCTTGGAATTTCTACCGCACTTGGCAGCATCCCGCATTGACTTGAAAAGTGCGGTCAAATCTGAAGGCGTTTCTCCTCTTTCCGAAGGCGAACGGCAACGTATtgt                                                                                                                                                    <  1:273496/144‑1 (MQ=255)                                                                                                                                                                                            caacaaACCCATGGCGACTATGCCAAATGGGCAAAAGTCTTGGAATTTCTACCGCACTTGGCAGCATCCCGCATTGACTTGAAAAGTGCGGTCAAATCTGAAAGCGTTTCTCCTCTTTCCGAAgg                                                                                                                                                                      >  1:17077/1‑125 (MQ=255)                                                                                                                                                                                            caacaaACCCATGGCGACTATGCCAAATGGGCAAAAGTCTTGGAATTTCTACCGCACTTGGCAGCATCCCGCATTGACTTGAAAAGTGCGGTCAAATCTGAAAGCGTTTCTCCTCTTTCCGAAGGCGAACGGCAACGTATTGTGc                                                                                                                                                  >  1:895500/1‑145 (MQ=255)                                                                                                                                                                                            caacaaACCCATGGCGACTATGCCAAATGGGCAAAAGTCTTGGAATTTCTACCGCACTTGGCAGCATCCCGCATTGACTTGAAAAGTGCGGTCAAATCTGAAAGCGTTTCTCCTCTTTCCGAAGGCGAACGGCAACGTATTGTGCACCACCTCAAACAGCTCATGCCGTGGCGTAAAGGCCCCTATGATTTGCTTGGCATTCATGTGGATTGCGAATGGCGCTCCGATTTCAAATGGGATCGCGtgctgc                                         >  1:828691/1‑250 (MQ=255)                                                                                                                                                                                                 aaCCCATGGCGACTATGCCAAATGGGCAAAAGTCTTGGAATTTCTACCGCACTTGGCAGCATCCCGCATTGACTTGAAAAGTGCGGTCAAATCTGAAAGCGTTTCTCCTCTTTCCGAAGGCGAACGGCAACGTATTGTGCACCACCTCAAACAGCTCATGCCGTGGCGTAAAGGCCCCTATGATTTGCTTGGCATTCATGTGGATTGCGAATGGCGCTCCGATTTCAAATGGGATCGCGTGCTGCCCCAt                                    >  1:699423/1‑250 (MQ=255)                                                                                                                                                                                                   cccATGGCGACTATGCCAAATGGGCAAAAGTCTTGGAATTTCTACCGCACTTGGCAGCATCCCGCATTGACTTGAAAAGTGCGGTCAAATCTGAAAGCGTTTCTCCTCTTTCCGAAGGCGAACGGCAACGTATTGTGCACCACCTCAAACAGCTCATGCCGTGGCGTAAAGGCCCCTATGAt                                                                                                      >  1:251941/1‑182 (MQ=255)                                                                                                                                                                                                     cATGGCGACTATGCCAAATGGGCAAAAGTCTTGGAATTTCTACCGCACTTGGCAGCATCCCGCATTGACTTGAAAAGTGCGGTCAAATCTGAAAGCGTTTCTCCTCTTTCCGAAGGCGAACGGCAACGTATTGTGCACCACCTCAAACAGCTCATGCCGTGGCGTAAAGGCCCCTATGATTTGCTTGGCATTCATGTGGATTGCGAATGGCGCTCCGATTTCAAATGGGATCGCGTGCTGCCCCATCTTg                                >  1:495612/1‑250 (MQ=255)                                                                                                                                                                                                               aTGCCAAATGGGCAAAAGTCTTGGAATTTCTACCGCACTTGGCAGCATCCCGCATTGACTTGAAAAGTGCGGTCAAATCTGAAAGCGTTTCTCCTCTTTCCGAAGGCGAACGGCAACGTATTGTGCACCACCTCAAACAGCTCATGCCGTGGCGTAAAGGCCCCTATGATTTGCTTGGCATTCATGTGGATTGCGAATGGCGCTCCGATTTCAAATGGGATCGCGTGCTGCCCCATCTTGCAc                             <  1:679240/243‑1 (MQ=255)                                                                                                                                                                                                               aTGCCAAATGGGCAAAAGTCTTGGAATTTCTACCGCACTTGGCAGCATCCCGCATTGACTTGAAAAGTGCGGTCAAATCTGAAAGCGTTTCTCCTCTTTCCGAAGGCGAACGGCAACGTATTGTGCACCACCTCAAACAGCTCATGCCGTGGCGTAAAGGCCCCTATGATTTGCTTGGCATTCATGTGGATTGCGAATGGCGCTCCGATTTCAAATGGGATCGCGTGCTGCCCCATCTTGCACCGTTGCAg                     >  1:705495/1‑251 (MQ=255)                                                                                                                                                                                                                tGCCAAATGGGCAAAAGTCTTGGAATTTCTACCGCACTTGGCAGCATCCCGCATTGACTTGaaa                                                                                                                                                                                                               <  1:180426/64‑1 (MQ=255)                                                                                                                                                                                                                  ccAAATGGGCAAAAGTCTTGGAATTTCTACCGCACTTGGCAGCATCCCGCATTGACTTGAAAAGTGCGGTCAAATCTGAAAGCGTTTCTCCTCTTTCCGAAGGCGAACGGCAACGTATTGTGCACCACCTCAAACAGCTCATGCCGTGGCGTAAAGGCCCCTATGATTTGCTTGGCATTCATGTGGATTGCGAATGGCGCTCCGATTTCAAATGGGATCGCGTGCTGCCCCATCTTGCACCGTTGCAGGAt                  <  1:223139/251‑1 (MQ=255)                                                                                                                                                                                                                        gggCAAAAGTCTTGGAATTTCTACCGCACTTGGCAGCATCCCGCATTGACTTGAAAAGTGCGGTCAAATCTGAAAGCGTTTCTCCTCTTTCCGAAGGCGAACGGCAACGTATTGTGCACCACCTCAAACAGCTCATGCCGTGGCGTAAAGGCCCCTATGATTTGCTTGGCATTCATGTGGATTGCGAATGGCGCTCCGATTTCAAATGGGATCGCGTGCTGCCCCATCTTGCATCGTTGCAGGATCGCatt            >  1:345678/1‑251 (MQ=255)                                                                                                                                                                                                                            aaaaGTCTTGGAATTTCTACCGCACTTGGCAGCATCCCGCATTGACTTGAAAAGTGCGGTCAAATCTGAAAGCGTTTCTCCTCTTTCCGAAGGCGAACGGCAACGTATTGTGCACCACCTCAAACAGCTCATGCCGTGGCGTAAAGGCCCCTATGATTTGCTTGGCATTCATGTGGATTGCGAATGGCGCTCCGATTTCAAATGGGATCGCGTGCTGCCCCATCTTGCACCGTTGCAGGATCGCATTAttt        <  1:502714/251‑1 (MQ=255)                                                                                                                                                                                                                                gTCTTGGAATTTCTACCGCACTTGGCAGCATCCCGCATTGACTTGAAAAGTGCGGTCAAATCTGAAAGCGTTTCTCCTCTTTCCGAAGGCGAACGGCAACGTATTGTGCACCACCTCAAACAGCTCATGCCGTGGCGTAAAGGCCCCTATGATTTGCTTGGCATTCATGTGGATTGCGAATGGCGCTCCGATTTCAAATGGGATCGCGTGCTGccc                                       <  1:209982/216‑1 (MQ=255)                                                                                                                                                                                                                                gTCTTGGAATTTCTACCGCACTTGGCAGCATCCCGCATTGACTTGAAAAGTGCGGTCAAATCTGAAAGCGTTTCTCCTCTTTCCGAAGGCGAACGGCAACGTATTGTGCACCACCTCAAACAGCTCATGCCGTGGCGTAAAGGCCCCTATGATTTGCTTGGCATTCATGTGGATTGCGAATGGCGCTCCGATTTCAAATGGGATCGCGTGCTGCCCCATCTTGCAc                             >  1:301557/1‑226 (MQ=255)                                                                                                                                                                                                                                gTCTTGGAATTTCTACCGCACTTGGCAGCATCCCGCATTGACTTGAAAAGTGCGGTCAAATCTGAAAGCGTTTCTCCTCTTTCCGAAGGCGAACGGCAACGTATTGTGCACCACCTCAAACAGCTCATGCCGTGGCGTAAAGGCCCCTATGATTTGCTTGGCATTCATGTGGATTGCGAATGGCGCTCCGATTTCAAATGGGATCGCGTGCTGCCCCATCTTGCACCGTTGCAGGATCGCATTATTTTAGa    >  1:561104/1‑251 (MQ=255)                                                                                                                                                                                                                                gTCTTGGAATTTCTACCGCACTTGGCAGCATCCCGCATTGACTTGAAAAGTGCGGTCAAATCTGAAAGCGTTTCTCCTCTTTCCGAAGGCGAACGGCAACGTATTGTGCACCACCTCAAACAGCTCATGCCGTGGCGTAAAGGCCCCTATGATTTGCTTGGCATTCATGTGGATTGCGAATGGCGCTCCGATTTCAAATGGGATCGCGTGCTGCCCCATCTTGCACCGTTGCAGGATCGCATTATTTTAGa    >  1:798578/1‑251 (MQ=255)                                                                                                                                                                                                                                 tCTTGGAATTTCTACCGCACTTGGCAGCATCCCGCATTGACTTGAAAAGTGCGGTCAAATCTGAAAGCGTTTCTCCTCTTTCCGAAGGCGAACGGCAACGTATTGTGCACCACCTCAAACAGCTCATGCCGTGGCGTAAAGGCCCCTATGATTTGCTTGGCATTCATGTGGATTGCGAATGGCGCTCCGATTTCAAATGGGATCGCGTGCTGCCCCATCTTGCACCGTTGCAGGATCGCATTATTTTAGa    <  1:575254/250‑1 (MQ=255)                                                                                                                                                                                                                                  cTTGGAATTTCTACCGCACTTGGCAGCATCCCGCATTGACTTGAAAAGTGCGGTCAAATCTGAAAGCGTTTCTCCTCTTTCCGAAGGCGAACGGCAACGTATTGTGCACCACCTCAAACAGCTCATGCCGTGGCGTAAAGGCCCCTATGATTTGCTTGGCATTCATGTGGATTGCGAATGGCGCTCCGATTTCAAATGGGATCGCGTGCTGCCCCATCTTGCACCGTTGCAGGATCGCATTATTTTAGATg  <  1:372006/251‑1 (MQ=255)                                                                                                                                                                                                                                      gAATTTCTACCGCACTTGGCAGCATCCCGCATTGACTTGAAAAGTGCGGTCAAATCTGAAAGCGTTTCTCCTCTTTCCGAAGGCGAACGGCAACGTATTGTGCACCACCTCAAACAGCTCATGCCGTGGCGTAAAGGCCCCTATGATTTGCTTGGCATTCATGTGGATTGCGAATGGCGCTCCGATTTCAAATGGGATCGCGTGCTGCCCCATCTTGCACCGTTGCAGGATCGCat             <  1:257242/236‑1 (MQ=255)                                                                                                                                                                                                                                            cTACCGCACTTGGCAGCATCCCGCATTGACTTGAAAAGTGCGGCCAAATCTGAAAGCGTTTCTCCTCTTTCCGAAGGCGAACGGCAACGTATTGTGCACCACCTCAAACAGCTCATGCCGTGGCGTAAAGGCCCCTATGATTTGCTTGGCATTCATGTGGATTGCGAATGGCGCTCCGATTTCAAATGGGATCGCGTGCTGCCCCATCt                                  >  1:630835/1‑209 (MQ=255)                                                                                                                                                                                                                                                   aCTTGGCAGCATCCCGCATTGACTTGAAAAGTGCGGTCAAATCTGAAAGCGTTTCTCCTCTTTCCGAAGGCGAACGGCAACGTATTGTGCACCACCTCAAACAGCTCATGCCGTGGCGTAAAGGCCCCTATGATTTGCTTGGCATTCATGTGGATTGCGAAt                                                                          >  1:471951/1‑162 (MQ=255)                                                                                                                                                                                                                                                   aCTTGGCAGCATCCCGCATTGACTTGAAAAGTGCGGTCAAATCTGAAAGCGTTTCTCCTCTTTCCGAAGGCGAACGGCAACGTATTGTGCACCACCTCAAACAGCTCATGCCGTGGCGTAAAGGCCCCTATGATTTGCTTGGCATTCATGTGGATTGCGAATGGCGCTCCGATTTCAAATGGGATCGCGTGCTGCCCCATCTTGCAcc                            <  1:831463/208‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                      TCGTTGCTGTCGGCAAAACCGGATGCGGAAATTACCGACACCTTTGACAAAGCCCAAAAGGCGTACAAAGAAGCTTTATAAGCGAGATAGCAAAATGATCGATTTTCGTCCTTTTTATCAACAAATCGCCACCACCAACCTTTCGCCATGGCTTGAAACGCTGCCCCTGCAACTCAAACAATGGCAGCAACAAACCCATGGCGACTATGCCAAATGGGCAAAAGTCTTGGAATTTCTACCGCACTTAGCAGCATCCCGCATTGACTTGAAAAGTGCGGTCAAATCTGAAAGCGTTTCTCCTCTTTCCGAAGGCGAACGGCAACGTATTGTGCACCACCTCAAACAGCTCATGCCGTGGCGTAAAGGCCCCTATGATTTGCTTGGCATTCATGTGGATTGCGAATGGCGCTCCGATTTCAAATGGGATCGCGTGCTGCCCCATCTTGCACCGTTGCAGGATCGCATTATTTTAGATG  >  1/1345472‑1345947

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑